PaperHeterologous overexpression and preliminary antimicrobial activity test of salmocin M, a novel colicin M-like bacteriocin against Salmonella sp.
Ewelina Łojewska; T. Sakowicz; M. Korycka-Machała et al. · 2022 · Archives of Microbiology · DOI
Full title: Heterologous overexpression and preliminary antimicrobial activity test of salmocin M, a novel colicin M-like bacteriocin against Salmonella sp.
Authors: Ewelina Łojewska; T. Sakowicz; M. Korycka-Machała; Tomasz Kowalczyk
PaperOnline monitoring of protein refolding in inclusion body processing using intrinsic fluorescence
Chika Linda Igwe; Don Fabian Müller; Florian Gisperg et al. · 2024 · Analytical and Bioanalytical Chemistry · DOI
Full title: Online monitoring of protein refolding in inclusion body processing using intrinsic fluorescence
Authors: Chika Linda Igwe; Don Fabian Müller; Florian Gisperg; J. N. Pauk; Matthias Kierein; Mohamed Elshazly; Robert Klausser; Julian Kopp; Oliver Spadiut; Eva Přáda Brichtová
PaperStrategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories: production of human alpha-galactosidase A
U. Unzueta; F. Vázquez; G. Accardi et al. · 2015 · Applied Microbiology and Biotechnology · DOI
Full title: Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories: production of human alpha-galactosidase A
Authors: U. Unzueta; F. Vázquez; G. Accardi; Rosa Mendoza; Verónica Toledo-Rubio; M. Giuliani; F. Sannino; E. Parrilli; I. Abasolo; S. Schwartz; M. Tutino; A. Villaverde; J. Corchero; N. Ferrer-Miralles
PaperIdentification of microRNAs specific for high producer CHO cell lines using steady-state cultivation
Andreas Maccani; M. Hackl; C. Leitner et al. · 2014 · Applied Microbiology and Biotechnology · DOI
Full title: Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation
Authors: Andreas Maccani; M. Hackl; C. Leitner; W. Steinfellner; A. Graf; N. Tatto; M. Karbiener; M. Scheideler; J. Grillari; D. Mattanovich; R. Kunert; N. Borth; R. Grabherr; W. Ernst
PaperDevelopment of a constitutive and an auto-inducible high-yield expression system for recombinant protein production in the microalga Nannochloropsis oceanica.
de Grahl, Imke; Rout, Sweta Suman; Maple-Grødem, Jodi et al. · 2020 · Applied microbiology and biotechnology · DOI
Full title: Development of a constitutive and an auto-inducible high-yield expression system for recombinant protein production in the microalga Nannochloropsis oceanica.
PaperTeaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli
D. Wurm; Julian Quehenberger; Julia Mildner et al. · 2017 · Applied Microbiology and Biotechnology · DOI
Full title: Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli
Authors: D. Wurm; Julian Quehenberger; Julia Mildner; B. Eggenreich; C. Slouka; A. Schwaighofer; K. Wieland; B. Lendl; Vignesh Rajamanickam; Christoph Herwig; O. Spadiut
PaperPredictive approaches to guide the expression of recombinant vaccine targets in Escherichia coli: a case study presentation utilising Absynth Biologics Ltd. proprietary Clostridium difficile vaccine antigens.
Hussain, Hirra; McKenzie, Edward A; Robinson, Andrew M et al. · 2021 · Applied microbiology and biotechnology · DOI
Full title: Predictive approaches to guide the expression of recombinant vaccine targets in Escherichia coli: a case study presentation utilising Absynth Biologics Ltd. proprietary Clostridium difficile vaccine antigens.
Authors: Hussain, Hirra; McKenzie, Edward A; Robinson, Andrew M; Gingles, Neill A; Marston, Fiona; Warwicker, Jim; Dickson, Alan J
PaperCharacterization of a novel + 70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry.
Sandberg, Magdalena Widgren; Bunkenborg, Jakob; Thyssen, Stine et al. · 2022 · Amino acids · DOI
Full title: Characterization of a novel + 70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry.
PaperPhosphate-inducible poly-hydroxy butyrate production dynamics in CO2 supplemented upscaled cultivation of engineered Phaeodactylum tricornutum
Matthias Windhagauer; Raffaela M. Abbriano; Dorothea A. Pittrich et al. · 2022 · Journal of Applied Phycology · DOI
Full title: Phosphate-inducible poly-hydroxy butyrate production dynamics in CO2 supplemented upscaled cultivation of engineered Phaeodactylum tricornutum
Authors: Matthias Windhagauer; Raffaela M. Abbriano; Dorothea A. Pittrich; M. Doblin
PaperCharacterization of Spent Yeast Streams from Precision Fermentations Driven by Genetically Engineered Yeasts: Valorization Potential for a Circular Bioeconomy
Deuchande, Teresa; Fundo, Joana F.; Rodrigues, Daniela et al. · 2024 · Waste and Biomass Valorization · DOI
Full title: Characterization of Spent Yeast Streams from Precision Fermentations Driven by Genetically Engineered Yeasts: Valorization Potential for a Circular Bioeconomy
Authors: Deuchande, Teresa; Fundo, Joana F.; Rodrigues, Daniela; Pintado, Manuela; Amaro, Ana L.
PaperMethanol addition after glucose depletion improves rPOXA 1B production under the pGap in P. pastoris X33: breaking the habit
Leidy D. Ardila-Leal; Raúl A. Poutou-Piñales; E. D. Morales-Álvarez et al. · 2021 · SN Applied Sciences · DOI
Full title: Methanol addition after glucose depletion improves rPOXA 1B production under the pGap in P. pastoris X33: breaking the habit
Authors: Leidy D. Ardila-Leal; Raúl A. Poutou-Piñales; E. D. Morales-Álvarez; Claudia M Rivera-Hoyos; A. M. Pedroza-Rodríguez; Balkys E. Quevedo-Hidalgo; Alejandro Pérez-Flórez
PaperSelection of CHO host and recombinant cell pools by inhibition of the proteasome results in enhanced product yields and cell specific productivity.
Knight, Tanya J; Turner, Sarah; Jaques, Colin M et al. · 2021 · Journal of biotechnology · DOI
Full title: Selection of CHO host and recombinant cell pools by inhibition of the proteasome results in enhanced product yields and cell specific productivity.
Authors: Knight, Tanya J; Turner, Sarah; Jaques, Colin M; Smales, C Mark
PaperDetection of antibodies against glucose 6-phosphate isomerase in synovial fluid of rheumatoid arthritis using surface plasmon resonance (BIAcore)
Ji-Yeon Kim; Mi-hong Lee; K. Jung et al. · 2003 · Experimental and Molecular Medicine · DOI
Full title: Detection of antibodies against glucose 6-phosphate isomerase in synovial fluid of rheumatoid arthritis using surface plasmon resonance (BIAcore)
Authors: Ji-Yeon Kim; Mi-hong Lee; K. Jung; H. Na; H. Cha; Eun-Mi Ko; Tae Jin Kim
PaperIndustrial brewing yeast engineered for the production of primary flavor determinants in hopped beer
Denby, Charles M.; Li, Rachel A.; Vu, Van Tien et al. · 2018 · Nature Communications · DOI
Full title: Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer
Authors: Denby, Charles M.; Li, Rachel A.; Vu, Van Tien; Costello, Zak; Lin, Weiyin; Chan, Leanne Jade G.; Williams, Joseph C.; Donaldson, Bryan; Bamforth, Charles W.; Petzold, Christopher J.; Scheller, Henrik Vibe; Martín, Héctor García; Keasling, Jay D.
PaperMultiplex secretome engineering enhances recombinant protein production and purity
S. Kol; Daniel Ley; T. Wulff et al. · 2019 · bioRxiv · DOI
Full title: Multiplex secretome engineering enhances recombinant protein production and purity
Authors: S. Kol; Daniel Ley; T. Wulff; Marianne Decker; Johnny Arnsdorf; J. Gutierrez; Austin W.T. Chiang; L. Pedersen; H. F. Kildegaard; G. Lee; N. Lewis
PaperCombinatorial optimization of gene expression through recombinase-mediated promoter and terminator shuffling in yeast
Charlotte Cautereels; Jolien Smets; Peter Bircham et al. · 2024 · Nature Communications · DOI
Full title: Combinatorial optimization of gene expression through recombinase-mediated promoter and terminator shuffling in yeast
Authors: Charlotte Cautereels; Jolien Smets; Peter Bircham; Dries De Ruysscher; Anna Zimmermann; Peter De Rijk; Jan Steensels; Anton Gorkovskiy; J. Masschelein; K. Verstrepen
PaperEdible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit
Rekdal, Vayu Maini; Luijt, Casper Robert Balten van der; Chen, Yan et al. · 2024 · Nature Communications · DOI
Full title: Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit
Authors: Rekdal, Vayu Maini; Luijt, Casper Robert Balten van der; Chen, Yan; Kakumanu, Ramu; Baidoo, Edward E. K.; Petzold, Christopher J.; Cruz‐Morales, Pablo; Keasling, Jay D.
PaperGenomic and functional analyses of fungal and bacterial consortia that enable lignocellulose breakdown in goat gut microbiomes
Peng, Xuefeng; Wilken, St. Elmo; Lankiewicz, Thomas S. et al. · 2021 · Nature Microbiology · DOI
Full title: Genomic and functional analyses of fungal and bacterial consortia that enable lignocellulose breakdown in goat gut microbiomes
Authors: Peng, Xuefeng; Wilken, St. Elmo; Lankiewicz, Thomas S.; Gilmore, Sean; Brown, Jennifer L.; Henske, John K.; Swift, Candice L.; Salamov, Asaf; Barry, Kerrie; Grigoriev, Igor V.; Theodorou, Michael K.; Valentine, David L.; O’Malley, Michelle
PaperHeterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans
R. Frandsen; Paiman Khorsand-Jamal; K. T. Kongstad et al. · 2018 · Scientific Reports · DOI
Full title: Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans
Authors: R. Frandsen; Paiman Khorsand-Jamal; K. T. Kongstad; Majse Nafisi; R. Kannangara; D. Staerk; F. Okkels; K. Binderup; B. Madsen; B. Møller; U. Thrane; U. Mortensen
PaperCrucial role of 4-deoxy-L-erythro-5-hexoseulose uronate reductase for alginate utilization revealed by adaptive evolution in engineered Saccharomyces cerevisiae
Fumiya Matsuoka; M. Hirayama; Takayuki Kashihara et al. · 2017 · Scientific Reports · DOI
Full title: Crucial role of 4-deoxy-L-erythro-5-hexoseulose uronate reductase for alginate utilization revealed by adaptive evolution in engineered Saccharomyces cerevisiae
Authors: Fumiya Matsuoka; M. Hirayama; Takayuki Kashihara; Hideki Tanaka; W. Hashimoto; K. Murata; S. Kawai
PaperA manually curated compendium of expression profiles for the microbial cell factory Corynebacterium glutamicum
Angela Kranz; T. Polen; Christian Kotulla et al. · 2022 · Scientific Data · DOI
Full title: A manually curated compendium of expression profiles for the microbial cell factory Corynebacterium glutamicum
Authors: Angela Kranz; T. Polen; Christian Kotulla; Annette Arndt; Graziella Bosco; Michael Bussmann; A. Chattopadhyay; A. Cramer; Cedric-Farhad Davoudi; Ursula Degner; Ramon Diesveld; Raphael Freiherr von Boeselager; Kim Gärtner; Cornelia Gätgens; T. Georgi; Christian Geraths; S. Haas; A. Heyer; Max Hünnefeld; T. Ishige; Armin Kabus; Nicolai Kallscheuer; Larissa Kever; Simon Klaffl; Britta Kleine; M. Kočan; A. Koch-Koerfges; K. Kraxner; Andreas Krug; Aileen Krüger; A. Küberl; Mohamed Labib; C. Lange; C. Mack; Tomoya Maeda; R. Mahr; Stephan Majda; Andrea Michel; Xenia Morosov; O. Müller; A. Nanda; J. Nickel; Jennifer Pahlke; E. Pfeifer; Laura Platzen; Paul Ramp; D. Rittmann; S. Schaffer; Sandra Scheele; Stephanie Spelberg; J. Schulte; J. Schweitzer; Georg Sindelar; Ulrike Sorger-Herrmann; Markus Spelberg; C. Stansen; Apilaasha Tharmasothirajan; J. V. Ooyen; Philana V van Summeren-Wesenhagen; M. Vogt; Sabrina Witthoff; Lingfeng Zhu; B. Eikmanns; M. Oldiges; Georg Schaumann; M. Baumgart; M. Brocker; L. Eggeling; R. Freudl; Julia Frunzke; Jan Marienhagen; V. Wendisch; M. Bott
PaperIdentification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Xiaowei Yu; Wei-Hong Sun; Ying-Zheng Wang et al. · 2017 · Scientific Reports · DOI
Full title: Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Authors: Xiaowei Yu; Wei-Hong Sun; Ying-Zheng Wang; Yan Xu
PaperStructural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells
C. R. Arbeitman; G. Auge; M. Blaustein et al. · 2020 · Scientific Reports · DOI
Full title: Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells
Authors: C. R. Arbeitman; G. Auge; M. Blaustein; L. Bredeston; E. Corapi; P. Craig; L. A. Cossio; L. Dain; C. D’Alessio; Fernanda Elias; Natalia B Fernández; J. Gasulla; Natalia Gorojovsky; Gustavo E. Gudesblat; Maria Georgina Herrera; L. I. Ibañez; Tommy Idrovo; Matías Iglesias Randon; L. Kamenetzky; A. Nadra; D. Noseda; C. Pavan; M. Pavan; M. F. Pignataro; Ernesto A. Román; L. Ruberto; N. Rubinstein; Javier Santos; F. Duarte; A. Zelada
PaperPredicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden
D. Wollborn; L. Munkler; Rebekka Horstmann et al. · 2022 · Scientific Reports · DOI
Full title: Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden
Authors: D. Wollborn; L. Munkler; Rebekka Horstmann; Andrea Germer; L. Blank; J. Büchs
PaperArtificial intelligence-based optimization for extracellular L-glutaminase free L-asparaginase production by Streptomyces violaceoruber under solid state fermentation conditions
Full title: Artificial intelligence-based optimization for extracellular L-glutaminase free L-asparaginase production by Streptomyces violaceoruber under solid state fermentation conditions
PaperA galactose-based auto-expression system improves T7-inducible protein production in Escherichia coli
James Bosco; Emily Gagliano; Kassandra L Boshae et al. · 2025 · Scientific Reports · DOI
Full title: A galactose-based auto-expression system improves T7-inducible protein production in Escherichia coli
Authors: James Bosco; Emily Gagliano; Kassandra L Boshae; John P. Statz; Timothy B Wheeler; DeAnna Cuello; Ashlyn Sliter; Christian Newby; Bernice Lin; Aysha Demeler; C. L. Pierpont; Cindee Yates-Hansen; M. Sydor; M. Ferrini; Kellie C Kuch; Brandon S. Cooper; Beverly J Piggott; Sarah J Certel; Kasper B. Hansen; Stephen R. Sprang; Bruce E. Bowler; Levi McClelland; M. Berkmen; Ekaterina Voronina
PaperA self-inducible heterologous protein expression system in Escherichia coli
L. Briand; Guillaume Marcion; Guillaume Marcion et al. · 2016 · Scientific Reports · DOI
Full title: A self-inducible heterologous protein expression system in Escherichia coli
Authors: L. Briand; Guillaume Marcion; Guillaume Marcion; Alexandre Kriznik; J. Heydel; Yves Artur; Carmen Garrido; Carmen Garrido; R. Seigneuric; R. Seigneuric; F. Neiers
PaperN-hypermannose glycosylation disruption enhances recombinant protein production by regulating secretory pathway and cell wall integrity in Saccharomyces cerevisiae
Hongting Tang; Shenghuan Wang; Jiajing Wang et al. · 2016 · Scientific Reports · DOI
Full title: N-hypermannose glycosylation disruption enhances recombinant protein production by regulating secretory pathway and cell wall integrity in Saccharomyces cerevisiae
Authors: Hongting Tang; Shenghuan Wang; Jiajing Wang; Meihui Song; Mengyang Xu; Mengying Zhang; Yu Shen; Jin Hou; X. Bao
PaperQuantitative analysis of carbohydrate residues in dextran 40 from various sources: a comparative study using high-performance liquid chromatography coupled with a charged aerosol detector
Xie, Siwei; Jin, Zhuyu; Huang, Yan et al. · 2025 · Analytical Methods · DOI
Full title: Quantitative analysis of carbohydrate residues in dextran 40 from various sources: a comparative study using high-performance liquid chromatography coupled with a charged aerosol detector
PaperGreen initiatives for the synthesis of polyamide monomers: precision fermentation using engineered Corynebacterium glutamicum and extraction of purified 5-aminovaleric acid (5AVA) and putrescine
Keerthi Sasikumar; Volker F. Wendisch; K. Nampoothiri · 2025 · RSC Sustainability · DOI
Full title: Green initiatives for the synthesis of polyamide monomers: precision fermentation using engineered Corynebacterium glutamicum and extraction of purified 5-aminovaleric acid (5AVA) and putrescine
Authors: Keerthi Sasikumar; Volker F. Wendisch; K. Nampoothiri
PaperA genome-scale metabolic network model synergizes with statistical learning to predict amino acid concentrations in Chinese Hamster Ovary cell cultures
Song-Min Schinn; Carly Morrison; Wei Wei et al. · 2020 · bioRxiv · DOI
Full title: A genome-scale metabolic network model synergizes with statistical learning to predict amino acid concentrations in Chinese Hamster Ovary cell cultures
Authors: Song-Min Schinn; Carly Morrison; Wei Wei; Lin Zhang; Nathan E. Lewis
PaperA High Throughput Assay for Measuring Secreted Protein Based on a de novo Fluorescent Reporter Reveals Regulatory and Structural Insights in Salmonella Type Three Secretion System.
Leach, Samuel Alexander; Summers, Jordan Scott; Wen, Edward et al. · 2025 · bioRxiv : the preprint server for biology · DOI
Full title: A High Throughput Assay for Measuring Secreted Protein Based on a de novo Fluorescent Reporter Reveals Regulatory and Structural Insights in Salmonella Type Three Secretion System.
Authors: Leach, Samuel Alexander; Summers, Jordan Scott; Wen, Edward; Tullman-Ercek, Danielle
PaperSynthetic ratio computation for programming population composition and multicellular morphology
B. An; Tzu-Chieh Tang; Qian Zhang et al. · 2025 · bioRxiv · DOI
Full title: Synthetic ratio computation for programming population composition and multicellular morphology
Authors: B. An; Tzu-Chieh Tang; Qian Zhang; Teng Wang; Yanyi Wang; Kesheng Gan; Kun Liu; Dan Zhang; Yuzhu Liu; Yukui Pan; Min Yu; William M. Shaw; Qianyi Liang; Yaomin Wang; Chunbo Lou; Timothy K. Lu; George M. Church; Chao Zhong
PaperNatural allelic variations of <i>Saccharomyces cerevisiae</i> impact stuck fermentation due to the combined effect of ethanol and temperature; a QTL-mapping study
Marullo, Philippe; Durrens, Pascal; Peltier, Émilien et al. · 2019 · DOI
Full title: Natural allelic variations of <i>Saccharomyces cerevisiae</i> impact stuck fermentation due to the combined effect of ethanol and temperature; a QTL-mapping study
PaperSustainable production of plastic-degrading enzymes in Chlamydomonas pacifica
C. Diaz; João Vitor Dutra Molino; Barbara Saucedo et al. · 2025 · bioRxiv · DOI
Full title: Sustainable production of plastic-degrading enzymes in Chlamydomonas pacifica
Authors: C. Diaz; João Vitor Dutra Molino; Barbara Saucedo; K. Kang; Évellin do Espirito Santo; Marissa Tessman; Abhishek Gupta; Michael D. Burkart; Ryan Simkovsky; S. Mayfield
PaperEnhanced recombinant protein production and differential expression of molecular chaperones in sf-caspase-1-repressed stable cells after baculovirus infection.
Lai, Yiu-Kay; Hsu, John T-A; Chu, Chih-Chieh et al. · 2012 · BMC biotechnology · DOI
Full title: Enhanced recombinant protein production and differential expression of molecular chaperones in sf-caspase-1-repressed stable cells after baculovirus infection.
PaperGenome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1
N. Ravin; M. Eldarov; V. Kadnikov et al. · 2013 · BMC Genomics · DOI
Full title: Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1
Authors: N. Ravin; M. Eldarov; V. Kadnikov; A. Beletsky; J. Schneider; E. Mardanova; E. Smekalova; Maria I Zvereva; O. Dontsova; A. Mardanov; K. Skryabin
PaperPre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli
V. D. Nguyen; Feras Hatahet; Kirsi E. H. Salo et al. · 2011 · Microbial Cell Factories · DOI
Full title: Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli
Authors: V. D. Nguyen; Feras Hatahet; Kirsi E. H. Salo; Eveliina Enlund; Chi Zhang; L. Ruddock
PaperEnhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch cultures.
Pilarek, Maciej; Glazyrina, Julia; Neubauer, Peter · 2011 · Microbial cell factories · DOI
Full title: Enhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch cultures.
Authors: Pilarek, Maciej; Glazyrina, Julia; Neubauer, Peter
PaperDevelopment of a cultivation process for the enhancement of human interferon alpha 2b production in the oleaginous yeast, Yarrowia lipolytica.
Gasmi, Najla; Ayed, Atef; Ammar, Billel Bel Hadj et al. · 2011 · Microbial cell factories · DOI
Full title: Development of a cultivation process for the enhancement of human interferon alpha 2b production in the oleaginous yeast, Yarrowia lipolytica.
Full title: Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette.
PaperDecrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures.
Vanz, Ana Letícia; Nimtz, Manfred; Rinas, Ursula · 2014 · Microbial cell factories · DOI
Full title: Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures.
Authors: Vanz, Ana Letícia; Nimtz, Manfred; Rinas, Ursula
PaperOperational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: a review.
Cos, Oriol; Ramón, Ramón; Montesinos, José Luis et al. · 2006 · Microbial cell factories · DOI
Full title: Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: a review.
Authors: Cos, Oriol; Ramón, Ramón; Montesinos, José Luis; Valero, Francisco
PaperCharacterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis
Frick, Oliver; Wittmann, Christoph · 2005 · Microbial Cell Factories · DOI
Full title: Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis
PaperThe small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies.
Lethanh, Ha; Neubauer, Peter; Hoffmann, Frank · 2005 · Microbial cell factories · DOI
Full title: The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies.
Authors: Lethanh, Ha; Neubauer, Peter; Hoffmann, Frank
PaperIdentification of anthranilate and benzoate metabolic operons of Pseudomonas fluorescens and functional characterization of their promoter regions.
Retallack, Diane M; Thomas, Tracey C; Shao, Ying et al. · 2006 · Microbial cell factories · DOI
Full title: Identification of anthranilate and benzoate metabolic operons of Pseudomonas fluorescens and functional characterization of their promoter regions.
Authors: Retallack, Diane M; Thomas, Tracey C; Shao, Ying; Haney, Keith L; Resnick, Sol M; Lee, Vincent D; Squires, Charles H
PaperApplication of a wide-range yeast vector (CoMed) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts.
Steinborn, Gerhard; Böer, Erik; Scholz, Anja et al. · 2006 · Microbial cell factories · DOI
Full title: Application of a wide-range yeast vector (CoMed) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts.
PaperSimple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen.
Gurramkonda, Chandrasekhar; Adnan, Ahmad; Gäbel, Thomas et al. · 2009 · Microbial cell factories · DOI
Full title: Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen.
PaperDeveloping a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime.
Holmes, William J; Darby, Richard Aj; Wilks, Martin Db et al. · 2009 · Microbial cell factories · DOI
Full title: Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime.
Authors: Holmes, William J; Darby, Richard Aj; Wilks, Martin Db; Smith, Rodney; Bill, Roslyn M
PaperA novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures.
Krause, Mirja; Ukkonen, Kaisa; Haataja, Tatu et al. · 2010 · Microbial cell factories · DOI
Full title: A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures.
PaperMacromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels.
Carnicer, Marc; Baumann, Kristin; Töplitz, Isabelle et al. · 2009 · Microbial cell factories · DOI
Full title: Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels.
PaperIncreasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory.
Ferndahl, Cecilia; Bonander, Nicklas; Logez, Christel et al. · 2010 · Microbial cell factories · DOI
Full title: Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory.
Authors: Ferndahl, Cecilia; Bonander, Nicklas; Logez, Christel; Wagner, Renaud; Gustafsson, Lena; Larsson, Christer; Hedfalk, Kristina; Darby, Richard A J; Bill, Roslyn M
PaperScale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations.
Full title: Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations.
PaperDifferential affects of low glucose on the macroheterogeneity and microheterogeneity of glycosylation in CHO-EG2 camelid monoclonal antibodies
Bo Liu; Carina Villacres-Barragan; E. Lattová et al. · 2013 · BMC Proceedings · DOI
Full title: Differential affects of low glucose on the macroheterogeneity and microheterogeneity of glycosylation in CHO-EG2 camelid monoclonal antibodies
Authors: Bo Liu; Carina Villacres-Barragan; E. Lattová; M. Spearman; M. Butler
PaperEukaryotic protein production in designed storage organelles
M. Torrent; B. Llompart; Sabine Lasserre-Ramassamy et al. · 2009 · BMC Biology · DOI
Full title: Eukaryotic protein production in designed storage organelles
Authors: M. Torrent; B. Llompart; Sabine Lasserre-Ramassamy; I. Llop-Tous; M. Bastida; P. Marzábal; A. Westerholm-Parvinen; M. Saloheimo; P. Heifetz; M. Ludevid
PaperMolecular characterization of cyclophilin A-like protein from Piriformospora indica for its potential role to abiotic stress tolerance in E. coli
D. Trivedi; M. W. Ansari; Tanima Dutta et al. · 2013 · BMC Research Notes · DOI
Full title: Molecular characterization of cyclophilin A-like protein from Piriformospora indica for its potential role to abiotic stress tolerance in E. coli
Authors: D. Trivedi; M. W. Ansari; Tanima Dutta; P. Singh; N. Tuteja
PaperFunctional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks.
Bawa, Zharain; Routledge, Sarah J; Jamshad, Mohammed et al. · 2014 · Microbial cell factories · DOI
Full title: Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks.
Authors: Bawa, Zharain; Routledge, Sarah J; Jamshad, Mohammed; Clare, Michelle; Sarkar, Debasmita; Dickerson, Ian; Ganzlin, Markus; Poyner, David R; Bill, Roslyn M
PaperCharacterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications.
Camattari, Andrea; Goh, Amelia; Yip, Lian Yee et al. · 2016 · Microbial cell factories · DOI
Full title: Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications.
PaperThe fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli.
Krause, Mirja; Neubauer, Antje; Neubauer, Peter · 2016 · Microbial cell factories · DOI
Full title: The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli.
Authors: Krause, Mirja; Neubauer, Antje; Neubauer, Peter
PaperIntegration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization.
Schwarzhans, Jan-Philipp; Wibberg, Daniel; Winkler, Anika et al. · 2016 · Microbial cell factories · DOI
Full title: Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization.
PaperConstitutively-stressed yeast strains are high-yielding for recombinant Fps1: implications for the translational regulation of an aquaporin.
Cartwright, Stephanie P; Darby, Richard A J; Sarkar, Debasmita et al. · 2017 · Microbial cell factories · DOI
Full title: Constitutively-stressed yeast strains are high-yielding for recombinant Fps1: implications for the translational regulation of an aquaporin.
Authors: Cartwright, Stephanie P; Darby, Richard A J; Sarkar, Debasmita; Bonander, Nicklas; Gross, Stephane R; Ashe, Mark P; Bill, Roslyn M
PaperMetabolic and proteomic analyses of product selectivity and redox regulation in Clostridium pasteurianum grown on glycerol under varied iron availability
C. Groeger; Wei Wang; W. Sabra et al. · 2017 · Microbial Cell Factories · DOI
Full title: Metabolic and proteomic analyses of product selectivity and redox regulation in Clostridium pasteurianum grown on glycerol under varied iron availability
Authors: C. Groeger; Wei Wang; W. Sabra; T. Utesch; A. Zeng
PaperMulticopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker.
Betancur, Maritza Ocampo; Reis, Viviane Castelo Branco; Nicola, André Moraes et al. · 2017 · Microbial cell factories · DOI
Full title: Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker.
Authors: Betancur, Maritza Ocampo; Reis, Viviane Castelo Branco; Nicola, André Moraes; De Marco, Janice Lisboa; de Moraes, Lídia Maria Pepe; Torres, Fernando Araripe Gonçalves
PaperImplications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.
Moser, Josef W; Prielhofer, Roland; Gerner, Samuel M et al. · 2017 · Microbial cell factories · DOI
Full title: Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.
Authors: Moser, Josef W; Prielhofer, Roland; Gerner, Samuel M; Graf, Alexandra B; Wilson, Iain B H; Mattanovich, Diethard; Dragosits, Martin
PaperDevelopment of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica.
Shrestha, Roshan P; Hildebrand, Mark · 2017 · Microbial cell factories · DOI
Full title: Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica.
PaperProduction of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks.
Valdez-Cruz, Norma A; Reynoso-Cereceda, Greta I; Pérez-Rodriguez, Saumel et al. · 2017 · Microbial cell factories · DOI
Full title: Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks.
PaperRNA-guided single/double gene repressions in Corynebacterium glutamicum using an efficient CRISPR interference and its application to industrial strain.
Park, Jaehyun; Shin, Hyojung; Lee, Sun-Mi et al. · 2018 · Microbial cell factories · DOI
Full title: RNA-guided single/double gene repressions in Corynebacterium glutamicum using an efficient CRISPR interference and its application to industrial strain.
Authors: Park, Jaehyun; Shin, Hyojung; Lee, Sun-Mi; Um, Youngsoon; Woo, Han Min
PaperCorrection to: Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae.
Wehrs, Maren; Prahl, Jan-Philip; Moon, Jadie et al. · 2019 · Microbial cell factories · DOI
Full title: Correction to: Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae.
PaperProduction of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison.
Pekarsky, Alexander; Veiter, Lukas; Rajamanickam, Vignesh et al. · 2018 · Microbial cell factories · DOI
Full title: Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison.
PaperFate of the UPR marker protein Kar2/Bip and autophagic processes in fed-batch cultures of secretory insulin precursor producing Pichia pastoris.
Roth, Gustavo; Vanz, Ana Letícia; Lünsdorf, Heinrich et al. · 2018 · Microbial cell factories · DOI
Full title: Fate of the UPR marker protein Kar2/Bip and autophagic processes in fed-batch cultures of secretory insulin precursor producing Pichia pastoris.
PaperConstruction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production.
Gawin, Agnieszka; Peebo, Karl; Hans, Sebastian et al. · 2019 · Microbial cell factories · DOI
Full title: Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production.
PaperDisruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris.
Marsalek, Lukas; Puxbaum, Verena; Buchetics, Markus et al. · 2019 · Microbial cell factories · DOI
Full title: Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris.
PaperEngineering transcription factor-based biosensors for repressive regulation through transcriptional deactivation design in Saccharomyces cerevisiae.
Qiu, Chenxi; Chen, Xiaoxu; Rexida, Reheman et al. · 2020 · Microbial cell factories · DOI
Full title: Engineering transcription factor-based biosensors for repressive regulation through transcriptional deactivation design in Saccharomyces cerevisiae.
PaperThe signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host's fluorescent intensity.
Gao, Jianhua; Qian, Hongmei; Guo, Xiaoqin et al. · 2020 · Microbial cell factories · DOI
Full title: The signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host's fluorescent intensity.
PaperGenome-scale metabolic modelling enables deciphering ethanol metabolism via the acrylate pathway in the propionate-producer Anaerotignum neopropionicum.
Benito-Vaquerizo, Sara; Parera Olm, Ivette; de Vroet, Thijs et al. · 2022 · Microbial cell factories · DOI
Full title: Genome-scale metabolic modelling enables deciphering ethanol metabolism via the acrylate pathway in the propionate-producer Anaerotignum neopropionicum.
Authors: Benito-Vaquerizo, Sara; Parera Olm, Ivette; de Vroet, Thijs; Schaap, Peter J; Sousa, Diana Z; Martins Dos Santos, Vitor A P; Suarez-Diez, Maria
PaperStrain specific properties of Escherichia coli can prevent non-canonical amino acid misincorporation caused by scale-related process heterogeneities.
Mayer, Florian; Cserjan-Puschmann, Monika; Haslinger, Benedikt et al. · 2022 · Microbial cell factories · DOI
Full title: Strain specific properties of Escherichia coli can prevent non-canonical amino acid misincorporation caused by scale-related process heterogeneities.
PaperEnhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography.
Carratalá, Jose Vicente; Atienza-Garriga, Jan; López-Laguna, Hèctor et al. · 2023 · Microbial cell factories · DOI
Full title: Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography.
Authors: Carratalá, Jose Vicente; Atienza-Garriga, Jan; López-Laguna, Hèctor; Vázquez, Esther; Villaverde, Antonio; Sánchez, Julieta M; Ferrer-Miralles, Neus
PaperSystematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity
Guangle Yu; Xiaochen Li; Qiuyue Duan et al. · 2023 · Microbial Cell Factories · DOI
Full title: Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity
Authors: Guangle Yu; Xiaochen Li; Qiuyue Duan; Jun Fu; Youming Zhang; Hailong Wang; Ji Luan
PaperA strategy to enhance and modify fatty acid synthesis in Corynebacterium glutamicum and Escherichia coli: overexpression of acyl-CoA thioesterases.
Liu, Jin; Mandlaa; Wang, Jia et al. · 2023 · Microbial cell factories · DOI
Full title: A strategy to enhance and modify fatty acid synthesis in Corynebacterium glutamicum and Escherichia coli: overexpression of acyl-CoA thioesterases.
PaperRNA-seq reveals multifaceted gene expression response to Fab production in Escherichia coli fed-batch processes with particular focus on ribosome stalling.
Vazulka, Sophie; Schiavinato, Matteo; Tauer, Christopher et al. · 2024 · Microbial cell factories · DOI
Full title: RNA-seq reveals multifaceted gene expression response to Fab production in Escherichia coli fed-batch processes with particular focus on ribosome stalling.
PaperCharacterization and optimization of 5´ untranslated region containing poly-adenine tracts in Kluyveromyces marxianus using machine-learning model.
Zeng, Junyuan; Song, Kunfeng; Wang, Jingqi et al. · 2024 · Microbial cell factories · DOI
Full title: Characterization and optimization of 5´ untranslated region containing poly-adenine tracts in Kluyveromyces marxianus using machine-learning model.
PaperInducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source.
Arai, Toshiharu; Wada, Mayumi; Nishiguchi, Hiroki et al. · 2023 · Microbial cell factories · DOI
Full title: Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source.
PaperYields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study.
Arauzo-Aguilera, Klaudia; Buscajoni, Luisa; Koch, Karin et al. · 2023 · Microbial cell factories · DOI
Full title: Yields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study.
PaperModifications of the 5' region of the CASPONTM tag's mRNA further enhance soluble recombinant protein production in Escherichia coli.
Köppl, Christoph; Buchinger, Wolfgang; Striedner, Gerald et al. · 2024 · Microbial cell factories · DOI
Full title: Modifications of the 5' region of the CASPONTM tag's mRNA further enhance soluble recombinant protein production in Escherichia coli.
PaperCorrection to: Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid.
Àvila-Cabré, Sílvia; Pérez-Trujillo, Míriam; Albiol, Joan et al. · 2024 · Microbial cell factories · DOI
Full title: Correction to: Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid.
PaperComparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacterium glutamicum and leaky Escherichia coli in batch and fed-batch processes.
Fritzsche, Stefanie; Hübner, Holger; Oldiges, Marco et al. · 2024 · Microbial cell factories · DOI
Full title: Comparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacterium glutamicum and leaky Escherichia coli in batch and fed-batch processes.
PaperNo two clones are alike: characterization of heterologous subpopulations in a transgenic cell line of the model diatom Phaeodactylum tricornutum.
Diaz-Garza, Aracely Maribel; Merindol, Natacha; Dos Santos, Karen Cristine Gonçalves et al. · 2024 · Microbial cell factories · DOI
Full title: No two clones are alike: characterization of heterologous subpopulations in a transgenic cell line of the model diatom Phaeodactylum tricornutum.
PaperMicrobial lipases: advances in production, purification, biochemical characterization, and multifaceted applications in industry and medicine.
Abdelaziz, Ahmed A; Abo-Kamar, Amal M; Elkotb, Esraa Sobhy et al. · 2025 · Microbial cell factories · DOI
Full title: Microbial lipases: advances in production, purification, biochemical characterization, and multifaceted applications in industry and medicine.
Authors: Abdelaziz, Ahmed A; Abo-Kamar, Amal M; Elkotb, Esraa Sobhy; Al-Madboly, Lamiaa A
PaperEnhanced L-tyrosine production in Bacillus amyloliquefaciens through alleviation of feedback Inhibition and coordinated pathway expression.
Zhao, Ziyue; Ji, Anying; Song, Can et al. · 2026 · Microbial cell factories · DOI
Full title: Enhanced L-tyrosine production in Bacillus amyloliquefaciens through alleviation of feedback Inhibition and coordinated pathway expression.
PaperModel-driven exploration of lactose and galactose metabolism via an oxidoreductive pathway in Sungouiella intermedia for cell factory applications.
Peri, Kameshwara V R; Domenzain, Iván; Alalam, Hanna D et al. · 2026 · Microbial cell factories · DOI
Full title: Model-driven exploration of lactose and galactose metabolism via an oxidoreductive pathway in Sungouiella intermedia for cell factory applications.
Authors: Peri, Kameshwara V R; Domenzain, Iván; Alalam, Hanna D; Pianale, Luca Torello; Rascón, Abril Valverde; Nielsen, Jens; Geijer, Cecilia
PaperDevelopment of an efficient heterologous protein expression platform in Aspergillus niger through genetic modification of a glucoamylase hyperproducing industrial strain.
Gou, Fufan; Liu, Dandan; Gong, Chaohui et al. · 2025 · Microbial cell factories · DOI
Full title: Development of an efficient heterologous protein expression platform in Aspergillus niger through genetic modification of a glucoamylase hyperproducing industrial strain.
PaperMetabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomer.
Sommer, Annalena; Pauli, Sarah; Kohlstedt, Michael et al. · 2026 · Microbial cell factories · DOI
Full title: Metabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomer.
PaperSystematic perturbation of cultures of K. phaffii by carbon co-feeding show gene signatures associated with production of recombinant proteins.
Acharya, Raghav; Hinckley, Joshua; Barry, Rachel et al. · 2026 · Microbial cell factories · DOI
Full title: Systematic perturbation of cultures of K. phaffii by carbon co-feeding show gene signatures associated with production of recombinant proteins.
PaperSignificantly enhancing recombinant alkaline amylase production in Bacillus subtilis by integration of a novel mutagenesis-screening strategy with systems-level fermentation optimization
Yingfang Ma; W. Shen; Xianzhong Chen et al. · 2016 · Journal of Biological Engineering · DOI
Full title: Significantly enhancing recombinant alkaline amylase production in Bacillus subtilis by integration of a novel mutagenesis-screening strategy with systems-level fermentation optimization
Authors: Yingfang Ma; W. Shen; Xianzhong Chen; Long Liu; Zhemin Zhou; Fei Xu; Haiquan Yang
PaperSimultaneous achievement of high ethanol yield and titer in Clostridium thermocellum
Tian, Liang; Papanek, Beth; Olson, Daniel G. et al. · 2016 · Biotechnology for Biofuels · DOI
Full title: Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum
Authors: Tian, Liang; Papanek, Beth; Olson, Daniel G.; Rydzak, Thomas; Holwerda, Evert K.; Zheng, Tianyong; Zhou, Jilai; Maloney, Marybeth; Jiang, Nannan; Giannone, Richard J.; Hettich, Robert L.; Guss, Adam M.; Lynd, Lee R.
PaperEffect of recombinant protein production and release on microalgal fitness and the impact of environmental conditions for localized therapeutic delivery.
Carvajal, Felipe; Vargas-Torres, Valentina; Becerra, Daniela et al. · 2025 · Journal of biological engineering · DOI
Full title: Effect of recombinant protein production and release on microalgal fitness and the impact of environmental conditions for localized therapeutic delivery.
PaperEngineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration.
Liu, Xin-Xin; Wang, Yao; Zhang, Jian-Hui et al. · 2024 · Biotechnology for biofuels and bioproducts · DOI
Full title: Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration.
PaperLactic acid bacteria as microbial cell factories for the in vivo delivery of therapeutic proteins as secretable TAT fusion products
Giorgio Medici; G. Candini; Nicola Mottolese et al. · 2025 · Journal of Biological Engineering · DOI
Full title: Lactic acid bacteria as microbial cell factories for the in vivo delivery of therapeutic proteins as secretable TAT fusion products
Authors: Giorgio Medici; G. Candini; Nicola Mottolese; B. Uguagliati; F. Trebbi; M. Loi; Angelica Marina Bove; S. Stojanov; Erika Esposito; Rosalba Vitagliano; Federica D'Amico; S. Turroni; Jessica Fiori; A. Berlec; S. Trazzi; Elisabetta Ciani
PaperEngineered Escherichia coli platforms for tyrosine-derivative production from phenylalanine using phenylalanine hydroxylase and tetrahydrobiopterin-regeneration system
Y. Satoh; K. Fukui; D. Koma et al. · 2023 · Biotechnology for Biofuels and Bioproducts · DOI
Full title: Engineered Escherichia coli platforms for tyrosine-derivative production from phenylalanine using phenylalanine hydroxylase and tetrahydrobiopterin-regeneration system
Authors: Y. Satoh; K. Fukui; D. Koma; Ning Shen; T. Lee
PaperCoupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast.
Peng, Bingyin; Plan, Manuel R; Carpenter, Alexander et al. · 2017 · Biotechnology for biofuels · DOI
Full title: Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast.
Authors: Peng, Bingyin; Plan, Manuel R; Carpenter, Alexander; Nielsen, Lars K; Vickers, Claudia E
PaperDecreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris.
Jiao, Jian; Wang, Shuai; Tian, Hui et al. · 2020 · AMB Express · DOI
Full title: Decreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris.
PaperHigh-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterization
Martin Kangwa; Vikas Yelemane; A. N. Polat et al. · 2015 · AMB Express · DOI
Full title: High-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterization
Authors: Martin Kangwa; Vikas Yelemane; A. N. Polat; K. D. D. Gorrepati; M. Grasselli; M. Fernández-Lahore
PaperProcess optimization for biosynthesis of pyruvate decarboxylase (PDC) and Neuberg’s ketol (PAC) from a novel Pichia cecembensis through response surface methodology
Mushtaq, Zareena; Mukhtar, Hamid · 2022 · Annals of Microbiology · DOI
Full title: Process optimization for biosynthesis of pyruvate decarboxylase (PDC) and Neuberg’s ketol (PAC) from a novel Pichia cecembensis through response surface methodology
PaperCorrection: Activity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein Production.
Ullah, Raheem; Shah, Majid Ali; Tufail, Soban et al. · 2016 · PloS one · DOI
Full title: Correction: Activity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein Production.
PaperAnalysis of 11,430 recombinant protein production experiments reveals that protein yield is tunable by synonymous codon changes of translation initiation sites.
Bhandari, Bikash K; Lim, Chun Shen; Remus, Daniela M et al. · 2021 · PLoS computational biology · DOI
Full title: Analysis of 11,430 recombinant protein production experiments reveals that protein yield is tunable by synonymous codon changes of translation initiation sites.
Authors: Bhandari, Bikash K; Lim, Chun Shen; Remus, Daniela M; Chen, Augustine; van Dolleweerd, Craig; Gardner, Paul P
PaperExploiting Nucleotide Composition to Engineer Promoters
M. Grabherr; Jens Pontiller; E. Mauceli et al. · 2011 · PLoS ONE · DOI
Full title: Exploiting Nucleotide Composition to Engineer Promoters
Authors: M. Grabherr; Jens Pontiller; E. Mauceli; W. Ernst; Martina Baumann; T. Biagi; Ross Swofford; Pamela Russell; M. Zody; F. Di Palma; K. Lindblad-Toh; R. Grabherr
PaperActivity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein Production.
Ullah, Raheem; Shah, Majid Ali; Tufail, Soban et al. · 2016 · PloS one · DOI
Full title: Activity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein Production.
PaperEvaluation of the recombinant protein Tp0965 of Treponema pallidum as candidate antigen for serological diagnosis of syphilis
A. Runina; Рунина Анастасия Владимировна; A. S. Starovoitova et al. · 2016 · DOI
Full title: Evaluation of the recombinant protein Tp0965 of Treponema pallidum as candidate antigen for serological diagnosis of syphilis
Authors: A. Runina; Рунина Анастасия Владимировна; A. S. Starovoitova; Старовойтова Анастасия Сергеевна; D. Deryabin; Дерябин Дмитрий Геннадьевич; A. Kubanov; Кубанов Алексей Алексеевич
PaperApplications of recombinant Pichia pastoris in the healthcare industry.
Weinacker, Daniel; Rabert, Claudia; Zepeda, Andrea B et al. · 2013 · Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · DOI
Full title: Applications of recombinant Pichia pastoris in the healthcare industry.
Authors: Weinacker, Daniel; Rabert, Claudia; Zepeda, Andrea B; Figueroa, Carolina A; Pessoa, Adalberto; Farías, Jorge G
Year: 2013
Venue: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
PaperComparative analysis of enzymatic activity of tannase in non-conventional yeasts to produce ellagic acid
Márquez-López, Anahí; Ramírez-Conejo, Juan David; Chávez-Parga, Ma. del Carmen et al. · 2019 · Food Science and Technology · DOI
Full title: Comparative analysis of enzymatic activity of tannase in non-conventional yeasts to produce ellagic acid
Authors: Márquez-López, Anahí; Ramírez-Conejo, Juan David; Chávez-Parga, Ma. del Carmen; Flores, Dora Cecilia Valencia; Jaramillo, Miguel Ángel Zamudio; Rodríguez, Horacio González; González‐Hernández, Juan Carlos
PaperPotential of lignocellulolytic biocatalysts of native and proposed genetically engineered microbial cell factories on jute fiber modification and jute waste recycling: A review
Somnath Das; Dipankar Ghosh · 2022 · Journal of Experimental Biology and Agricultural Sciences · DOI
Full title: Potential of lignocellulolytic biocatalysts of native and proposed genetically engineered microbial cell factories on jute fiber modification and jute waste recycling: A review
Authors: Somnath Das; Dipankar Ghosh
Year: 2022
Venue: Journal of Experimental Biology and Agricultural Sciences
PaperProcess Design, Simulation, and Techno-Economic Evaluation of Recombinant Human Lactoferrin Production via Precision Fermentation in Pichia pastoris with Chromatography-Free Downstream Processing
Kaipa, Vignesh Kumar; H, Vishal Yadav; S, Tejaswi et al. · 2026 · DOI
Full title: Process Design, Simulation, and Techno-Economic Evaluation of Recombinant Human Lactoferrin Production via Precision Fermentation in Pichia pastoris with Chromatography-Free Downstream Processing
Paper1P140 A novel expression system for high-level recombinant protein production in Escherichia coli(4. Protein engineering,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
Full title: 1P140 A novel expression system for high-level recombinant protein production in Escherichia coli(4. Protein engineering,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
Authors: E. Hojo; M. Kamiya; T. Aizawa; M. Miyazawa; Y. Kato; M. Demura; K. Kawano
PaperEnhanced Acetate Tolerance and Recombinant Protein Accumulation in Escherichia coli by Transgenic Expression of a Heat Shock Protein from Carrot (Daucus carota L.)
Minhye Kim; Eun-Mi Im; Yeh Jin Ahn · 2022 · Iranian Journal of Biotechnology · DOI
Full title: Enhanced Acetate Tolerance and Recombinant Protein Accumulation in Escherichia coli by Transgenic Expression of a Heat Shock Protein from Carrot (Daucus carota L.)
PaperThe Use of Transcription Terminators to Generate Transgenic Lines of Chinese Hamster Ovary Cells (CHO) with Stable and High Level of Reporter Gene Expression.
Gasanov, N B; Toshchakov, S V; Georgiev, P G et al. · 2015 · Acta naturae · DOI
Full title: The Use of Transcription Terminators to Generate Transgenic Lines of Chinese Hamster Ovary Cells (CHO) with Stable and High Level of Reporter Gene Expression.
Authors: Gasanov, N B; Toshchakov, S V; Georgiev, P G; Maksimenko, O G
PaperSelection of High Producers From Combinatorial Libraries for the Production of Recombinant Proteins in Escherichia coli and Vibrio natriegens
Joel Eichmann; M. Oberpaul; T. Weidner et al. · 2019 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Selection of High Producers From Combinatorial Libraries for the Production of Recombinant Proteins in Escherichia coli and Vibrio natriegens
Authors: Joel Eichmann; M. Oberpaul; T. Weidner; Doreen Gerlach; P. Czermak
Year: 2019
Venue: Frontiers in Bioengineering and Biotechnology
PaperMetabolomic Alterations Do Not Induce Metabolic Burden in the Industrial Yeast M2n[pBKD2-Pccbgl1]-C1 Engineered by Multiple δ-Integration of a Fungal β-Glucosidase Gene
L. Favaro; L. Cagnin; L. Corte et al. · 2019 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Metabolomic Alterations Do Not Induce Metabolic Burden in the Industrial Yeast M2n[pBKD2-Pccbgl1]-C1 Engineered by Multiple δ-Integration of a Fungal β-Glucosidase Gene
Authors: L. Favaro; L. Cagnin; L. Corte; L. Roscini; Fabio De Pascale; L. Treu; S. Campanaro; M. Basaglia; W. V. van Zyl; S. Casella; G. Cardinali
Year: 2019
Venue: Frontiers in Bioengineering and Biotechnology
PaperMembrane Modulation of Super-Secreting “midiBacillus” Expressing the Major Staphylococcus aureus Antigen – A Mass-Spectrometry-Based Absolute Quantification Approach
Minia Antelo-Varela; Rocío Aguilar Suárez; J. Bartel et al. · 2020 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Membrane Modulation of Super-Secreting “midiBacillus” Expressing the Major Staphylococcus aureus Antigen – A Mass-Spectrometry-Based Absolute Quantification Approach
Authors: Minia Antelo-Varela; Rocío Aguilar Suárez; J. Bartel; Margarita Bernal-Cabas; T. Stobernack; Thomas Sura; J. V. van Dijl; S. Maaß; D. Becher
Year: 2020
Venue: Frontiers in Bioengineering and Biotechnology
PaperGlobal Reprogramming of Gene Transcription in Trichoderma reesei by Overexpressing an Artificial Transcription Factor for Improved Cellulase Production and Identification of Ypr1 as an Associated Regulator
Fei Zhang; Jia-Xiang Li; V. Champreda et al. · 2020 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Global Reprogramming of Gene Transcription in Trichoderma reesei by Overexpressing an Artificial Transcription Factor for Improved Cellulase Production and Identification of Ypr1 as an Associated Regulator
Authors: Fei Zhang; Jia-Xiang Li; V. Champreda; Chen-Guang Liu; F. Bai; Xinqing Zhao
Year: 2020
Venue: Frontiers in Bioengineering and Biotechnology
PaperReverse Engineering Targets for Recombinant Protein Production in Corynebacterium glutamicum Inspired by a Fast-Growing Evolved Descendant.
Lee, Min Ju; Park, Jihoon; Park, Kyunghoon et al. · 2020 · Frontiers in bioengineering and biotechnology · DOI
Full title: Reverse Engineering Targets for Recombinant Protein Production in Corynebacterium glutamicum Inspired by a Fast-Growing Evolved Descendant.
Authors: Lee, Min Ju; Park, Jihoon; Park, Kyunghoon; Kim, Jihyun F; Kim, Pil
Year: 2020
Venue: Frontiers in bioengineering and biotechnology
PaperMicrobial Cell Factory for Efficiently Synthesizing Plant Natural Products via Optimizing the Location and Adaptation of Pathway on Genome Scale
Bo Yang; Xudong Feng; Chun Li · 2020 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Microbial Cell Factory for Efficiently Synthesizing Plant Natural Products via Optimizing the Location and Adaptation of Pathway on Genome Scale
Authors: Bo Yang; Xudong Feng; Chun Li
Year: 2020
Venue: Frontiers in Bioengineering and Biotechnology
PaperInnovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production.
Gasset, Arnau; Garcia-Ortega, Xavier; Garrigós-Martínez, Javier et al. · 2022 · Frontiers in bioengineering and biotechnology · DOI
Full title: Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production.
Authors: Gasset, Arnau; Garcia-Ortega, Xavier; Garrigós-Martínez, Javier; Valero, Francisco; Montesinos-Seguí, José Luis
Year: 2022
Venue: Frontiers in bioengineering and biotechnology
PaperContinuous Process Verification 4.0 application in upstream: adaptiveness implementation managed by AI in the hypoxic bioprocess of the Pichia pastoris cell factory.
Gasset, Arnau; Van Wijngaarden, Joeri; Mirabent, Ferran et al. · 2024 · Frontiers in bioengineering and biotechnology · DOI
Full title: Continuous Process Verification 4.0 application in upstream: adaptiveness implementation managed by AI in the hypoxic bioprocess of the Pichia pastoris cell factory.
Authors: Gasset, Arnau; Van Wijngaarden, Joeri; Mirabent, Ferran; Sales-Vallverdú, Albert; Garcia-Ortega, Xavier; Montesinos-Seguí, José Luis; Manzano, Toni; Valero, Francisco
Year: 2024
Venue: Frontiers in bioengineering and biotechnology
PaperEditorial: Protein Aggregation and Solubility in Microorganisms (Archaea, Bacteria and Unicellular Eukaryotes): Implications and Applications.
Skretas, Georgios; Ventura, Salvador · 2020 · Frontiers in microbiology · DOI
Full title: Editorial: Protein Aggregation and Solubility in Microorganisms (Archaea, Bacteria and Unicellular Eukaryotes): Implications and Applications.
PaperWhole Genome Sequencing Reveals Major Deletions in the Genome of M7, a Gamma Ray-Induced Mutant of Trichoderma virens That Is Repressed in Conidiation, Secondary Metabolism, and Mycoparasitism
S. Pachauri; P. D. Sherkhane; Vinay Kumar et al. · 2020 · Frontiers in Microbiology · DOI
Full title: Whole Genome Sequencing Reveals Major Deletions in the Genome of M7, a Gamma Ray-Induced Mutant of Trichoderma virens That Is Repressed in Conidiation, Secondary Metabolism, and Mycoparasitism
Authors: S. Pachauri; P. D. Sherkhane; Vinay Kumar; P. Mukherjee
PaperTranscriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a High-Cell-Density Fed-Batch Cultivation Strategy
Chengbo Zhang; Yu Ma; Huabiao Miao et al. · 2020 · Frontiers in Microbiology · DOI
Full title: Transcriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a High-Cell-Density Fed-Batch Cultivation Strategy
PaperSimultaneous determination of pentostatin and 2'-amino-2'-deoxyadenosine in fermentation broth by high performance liquid chromatography-tandem mass spectrometry
Zhao, Minmin; Zhang, Hongyu; Lou, Tingting et al. · 2021 · Chinese Journal of Chromatography · DOI
Full title: Simultaneous determination of pentostatin and 2'-amino-2'-deoxyadenosine in fermentation broth by high performance liquid chromatography-tandem mass spectrometry
PaperGene expression, purification, and functional characterization of recombinant conotoxin μ-TIIIA and TIIIAlaMut in Escherichia coli with clinical evaluation of antiwrinkle efficacy.
Mikiewicz, Diana; Mazurkiewicz-Pisarek, Anna; Janczewska, Magdalena et al. · 2025 · Biotechnologia · DOI
Full title: Gene expression, purification, and functional characterization of recombinant conotoxin μ-TIIIA and TIIIAlaMut in Escherichia coli with clinical evaluation of antiwrinkle efficacy.
PaperScale-Up Challenges and Optimization Strategies for Green Fluorescent Protein Production in E. coli JM109: A Shake Flask and Batch Fermentation Study
G. Miteu · 2026 · IPS Journal of Applied Microbiology and Biotechnology · DOI
Full title: Scale-Up Challenges and Optimization Strategies for Green Fluorescent Protein Production in E. coli JM109: A Shake Flask and Batch Fermentation Study
Authors: G. Miteu
Year: 2026
Venue: IPS Journal of Applied Microbiology and Biotechnology
ThesisSimulation and techno-economic analysis of production processes of novel generation L-Asparaginases using recombinant Escherichia coli and Pichia pastoris
Azzoni, Adriano Rodrigues · 2022 · Universidade de São Paulo – USP Digital Library · DOI
Full title: Simulation and techno-economic analysis of production processes of novel generation L-Asparaginases using recombinant Escherichia coli and Pichia pastoris
Authors: Azzoni, Adriano Rodrigues
Year: 2022
Venue: Universidade de São Paulo – USP Digital Library
ThesisEstratégias de indução, alimentação pós-indução e permeabilização de membrana em cultivo descontínuo alimentado de Escherichia coli BL21 (DE3) na produção de L-asparaginase II EcA (P40S/S206C)
Pessoa Junior, Adalberto · 2024 · Universidade de São Paulo – USP Digital Library · DOI
Full title: Estratégias de indução, alimentação pós-indução e permeabilização de membrana em cultivo descontínuo alimentado de Escherichia coli BL21 (DE3) na produção de L-asparaginase II EcA (P40S/S206C)
Authors: Pessoa Junior, Adalberto
Year: 2024
Venue: Universidade de São Paulo – USP Digital Library
ThesisHigh-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
Blanchard, Véronique; Gadkari, R.P; George, A.V.E. et al. · 2008 · Utrecht University · DOI
Full title: High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
ThesisCRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Dalvie, Neil C.; Lorgeree, Timothy R.; Yang, Yuchen et al. · 2024 · Massachusetts Institute of Technology (MIT) · DOI
Full title: CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Authors: Dalvie, Neil C.; Lorgeree, Timothy R.; Yang, Yuchen; Rodriguez-Aponte, Sergio A.; Whittaker, Charles A.; Hinckley, Joshua A.; Clark, John J.; Del Rosario, Amanda M.; Love, Kerry R.; Love, J. C.
Year: 2024
Venue: Massachusetts Institute of Technology (MIT)
ThesisComparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain
Brady, Joseph R; Whittaker, Charles A; Tan, Melody C et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI
Full title: Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain
Authors: Brady, Joseph R; Whittaker, Charles A; Tan, Melody C; Kristensen, D Lee; Ma, Duanduan; Dalvie, Neil C; Love, Kerry Routenberg; Love, J Christopher
Year: 2021
Venue: Massachusetts Institute of Technology (MIT)
ThesisAdaptation of Aglycosylated Monoclonal Antibodies for Improved Production in Komagataella phaffii
Yang, Yuchen; Dalvie, Neil C; Brady, Joseph R et al. · 2025 · Massachusetts Institute of Technology (MIT) · DOI
Full title: Adaptation of Aglycosylated Monoclonal Antibodies for Improved Production in Komagataella phaffii
Authors: Yang, Yuchen; Dalvie, Neil C; Brady, Joseph R; Naranjo, Christopher A; Lorgeree, Timothy; Rodriguez‐Aponte, Sergio A; Johnston, Ryan S; Tracey, Mary K; Elenberger, Carmen M; Lee, Eric; Tié, Mark; Love, Kerry R; Love, J Christopher
Year: 2025
Venue: Massachusetts Institute of Technology (MIT)
ThesisOptimizing recombinant protein production in Chlamydomonas reinhardtii: turning a model organism into a commercially relevant biotechnological host
Torres-Tiji, Yasin · 2021 · University of California, San Diego (UCSD) · DOI
Full title: Optimizing recombinant protein production in Chlamydomonas reinhardtii: turning a model organism into a commercially relevant biotechnological host
ThesisUnderstanding the bug that makes our drugs: a multi-OMICs approach to engineer Escherichia coli for improved production of recombinant periplasmic proteins in the biopharmaceutical industry.
Seleiro, Alice Saville · 2022 · White Rose eTheses (Leeds, Sheffield, York) · DOI
Full title: Understanding the bug that makes our drugs: a multi-OMICs approach to engineer Escherichia coli for improved production of recombinant periplasmic proteins in the biopharmaceutical industry.
Authors: Seleiro, Alice Saville
Year: 2022
Venue: White Rose eTheses (Leeds, Sheffield, York)
ThesisEplt4 Proteinaceous Elicitor Produced in Pichia pastoris Has a Protective Effect Against Cercosporidium sofinum Infections of Soybean Leaves
Wang, Y; Song, J; Wu, Y et al. · 2021 · University of Melbourne · DOI
Full title: Eplt4 Proteinaceous Elicitor Produced in Pichia pastoris Has a Protective Effect Against Cercosporidium sofinum Infections of Soybean Leaves
ThesisPERISCOPE-Opt: Machine learning-based prediction of optimal fermentation conditions and yields of recombinant periplasmic protein expressed in Escherichia coli
Packiam, KAR; Ooi, CW; Li, F et al. · 2022 · University of Melbourne · DOI
Full title: PERISCOPE-Opt: Machine learning-based prediction of optimal fermentation conditions and yields of recombinant periplasmic protein expressed in Escherichia coli
ThesisToward the production of milk-identical proteins using precision fermentation on agricultural waste substrates : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biological Sciences at Massey University, Albany, New Zealand
McIsaac, Emily Claire · 2023 · Massey University · DOI
Full title: Toward the production of milk-identical proteins using precision fermentation on agricultural waste substrates : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biological Sciences at Massey University, Albany, New Zealand
ThesisDevelopment of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
Manderson, Daniel · 2017 · Massey University · DOI
Full title: Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
ThesisSimple surface plasmon resonance bio-sensor for the quantification of recombinant human epidermal growth factor (rhEGF) in expanded bed recovery
Faizal Wong, Fadzlie Wong · 2010 · Universiti Putra Malaysia (PSAS IR) · DOI
Full title: Simple surface plasmon resonance bio-sensor for the quantification of recombinant human epidermal growth factor (rhEGF) in expanded bed recovery
ThesisFermentation strategies for production of hyaluronic acid through biosynthesis by streptococcus zooepidemicus and recombinant escherichia coli using stirred tank bioreactor
Lai, Zee Wei · 2013 · Universiti Putra Malaysia (PSAS IR) · DOI
Full title: Fermentation strategies for production of hyaluronic acid through biosynthesis by streptococcus zooepidemicus and recombinant escherichia coli using stirred tank bioreactor
ThesisAumento de escala para a produção de Proteína verde fluorescente melhorada (Enhanced Green Fluorescent Protein - EGFP) a partir de Escherichia coli recombinante em biorreator convencional
Sousa, Ana Paula Abuchain [UNESP] · 2019 · Universidade Estadual Paulista (UNESP) · DOI
Full title: Aumento de escala para a produção de Proteína verde fluorescente melhorada (Enhanced Green Fluorescent Protein - EGFP) a partir de Escherichia coli recombinante em biorreator convencional
ThesisEscalamiento descendente del proceso de producción de proteína heteróloga por termo-inducción de cultivos de alta densidad celular de Escherichia coli : estudio de la respuesta transcripcional al choque térmico
Caspeta, Luis · 2021 · Universidad Nacional Autónoma de México (UNAM) · DOI
Full title: Escalamiento descendente del proceso de producción de proteína heteróloga por termo-inducción de cultivos de alta densidad celular de Escherichia coli : estudio de la respuesta transcripcional al choque térmico
Authors: Caspeta, Luis
Year: 2021
Venue: Universidad Nacional Autónoma de México (UNAM)
ThesisEngineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein
Green, Charlotte A; Kamble, Nitin S.; Court, Elizabeth K. et al. · 2019 · University of Cambridge · DOI
Full title: Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein
Authors: Green, Charlotte A; Kamble, Nitin S.; Court, Elizabeth K.; Bryant, Owain J; Hicks, Matthew G.; Lennon, Christopher; Fraser, Gillian M; Wright, Phillip C; Stafford, Graham P
ThesisRecombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications.
Mital, Suraj; Christie, Graham; Dikicioglu, Duygu · 2022 · University of Cambridge · DOI
Full title: Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications.
ThesisL’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic
Cámara Rey, Elena · 2017 · Universitat Autònoma de Barcelona (TDX) · DOI
Full title: L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic
ThesisPhysiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess
Gasset Franch, Arnau · 2024 · Universitat Autònoma de Barcelona (TDX) · DOI
Full title: Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess
ThesisCombining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris
Nieto Taype, Miguel Angel · 2020 · Universitat Autònoma de Barcelona (TDX) · DOI
Full title: Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris
ThesisModel-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter
Barrigón de San Marcos, José Manuel · 2015 · Universitat Autònoma de Barcelona (TDX) · DOI
Full title: Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter
ThesisA step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches
Garcia Ortega, Xavier · 2016 · Universitat Autònoma de Barcelona (TDX) · DOI
Full title: A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches
ThesisMultidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris
Garrigós Martínez, Javier · 2021 · Universitat Autònoma de Barcelona (TDX) · DOI
Full title: Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris
PaperRecombinant protein production driven by the tryptophan promoter is tightly controlled in ICONE 200, a new genetically engineered E. coli mutant.
Laurent Chevalet; Alain Robert; F. Gueneau et al. · 2000 · Biotechnology and Bioengineering · DOI
Full title: Recombinant protein production driven by the tryptophan promoter is tightly controlled in ICONE 200, a new genetically engineered E. coli mutant.
Authors: Laurent Chevalet; Alain Robert; F. Gueneau; Jean-Yves Bonnefoy; T. Nguyen
PaperFrom industrial by‐products to value‐added compounds: the design of efficient microbial cell factories by coupling systems metabolic engineering and bioprocesses
A. T. Rangel; Jorge Mario Gómez Ramírez; A. G. González Barrios · 2020 · Biofuels, Bioproducts and Biorefining · DOI
Full title: From industrial by‐products to value‐added compounds: the design of efficient microbial cell factories by coupling systems metabolic engineering and bioprocesses
Authors: A. T. Rangel; Jorge Mario Gómez Ramírez; A. G. González Barrios
PaperDisruption of genes involved in CORVET complex leads to enhanced secretion of heterologous carboxylesterase only in protease deficient Pichia pastoris.
Marsalek, Lukas; Gruber, Clemens; Altmann, Friedrich et al. · 2017 · Biotechnology journal · DOI
Full title: Disruption of genes involved in CORVET complex leads to enhanced secretion of heterologous carboxylesterase only in protease deficient Pichia pastoris.
PaperPichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins.
Maccani, Andreas; Landes, Nils; Stadlmayr, Gerhard et al. · 2014 · Biotechnology journal · DOI
Full title: Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins.
PaperThe potential of random forest and neural networks for biomass and recombinant protein modeling in Escherichia coli fed-batch fermentations.
Melcher, Michael; Scharl, Theresa; Spangl, Bernhard et al. · 2015 · Biotechnology journal · DOI
Full title: The potential of random forest and neural networks for biomass and recombinant protein modeling in Escherichia coli fed-batch fermentations.
PaperHyperosmotic stimulus study discloses benefits in ATP supply and reveals miRNA/mRNA targets to improve recombinant protein production of CHO cells.
Pfizenmaier, Jennifer; Junghans, Lisa; Teleki, Attila et al. · 2016 · Biotechnology journal · DOI
Full title: Hyperosmotic stimulus study discloses benefits in ATP supply and reveals miRNA/mRNA targets to improve recombinant protein production of CHO cells.
PaperHigh Cell Density Perfusion Culture has a Maintained Exoproteome and Metabolome.
L. Zamani; Magnus Lundqvist; Ye Zhang et al. · 2018 · Biotechnology Journal · DOI
Full title: High Cell Density Perfusion Culture has a Maintained Exoproteome and Metabolome.
Authors: L. Zamani; Magnus Lundqvist; Ye Zhang; Magnus Åberg; F. Edfors; G. Bidkhori; Anna Lindahl; A. Mie; Adil Mardinoglu; R. Field; Richard Turner; Johan Rockberg; V. Chotteau
PaperMicroscale Perfusion-Based Cultivation for Pichia pastoris Clone Screening Enables Accelerated and Optimized Recombinant Protein Production Processes.
Totaro, Damiano; Radoman, Bojana; Schmelzer, Bernhard et al. · 2021 · Biotechnology journal · DOI
Full title: Microscale Perfusion-Based Cultivation for Pichia pastoris Clone Screening Enables Accelerated and Optimized Recombinant Protein Production Processes.
PaperInhibition of E. coli Host RNA Polymerase Allows Efficient Extracellular Recombinant Protein Production by Enhancing Outer Membrane Leakiness.
Kastenhofer, Jens; Rettenbacher, Lukas; Feuchtenhofer, Lukas et al. · 2021 · Biotechnology journal · DOI
Full title: Inhibition of E. coli Host RNA Polymerase Allows Efficient Extracellular Recombinant Protein Production by Enhancing Outer Membrane Leakiness.
PaperSupplementation of critical amino acids improves glycerol and lactose uptake and enhances recombinant protein production in Escherichia coli.
Kumar, Jashwant; Chauhan, Ashish S; Gupta, Jaya A et al. · 2021 · Biotechnology journal · DOI
Full title: Supplementation of critical amino acids improves glycerol and lactose uptake and enhances recombinant protein production in Escherichia coli.
PaperMonitoring of complex industrial bioprocesses for metabolite concentrations using modern spectroscopies and machine learning: Application to gibberellic acid production
McGovern, Aoife C.; Broadhurst, David; Taylor, Janet A. et al. · 2002 · Biotechnology and Bioengineering · DOI
Full title: Monitoring of complex industrial bioprocesses for metabolite concentrations using modern spectroscopies and machine learning: Application to gibberellic acid production
Authors: McGovern, Aoife C.; Broadhurst, David; Taylor, Janet A.; Kaderbhai, Naheed; Winson, Michael K.; Small, David A.P.; Rowland, Jem J.; Kell, Douglas B.; Goodacre, Royston
PaperQuantitative physiology of Pichia pastoris during glucose-limited high-cell density fed-batch cultivation for recombinant protein production.
Heyland, Jan; Fu, Jianan; Blank, Lars M et al. · 2010 · Biotechnology and bioengineering · DOI
Full title: Quantitative physiology of Pichia pastoris during glucose-limited high-cell density fed-batch cultivation for recombinant protein production.
Authors: Heyland, Jan; Fu, Jianan; Blank, Lars M; Schmid, Andreas
PaperDebottlenecking recombinant protein production in Bacillus megaterium under large-scale conditions--targeted precursor feeding designed from metabolomics.
Korneli, Claudia; Bolten, Christoph Josef; Godard, Thibault et al. · 2012 · Biotechnology and bioengineering · DOI
Full title: Debottlenecking recombinant protein production in Bacillus megaterium under large-scale conditions--targeted precursor feeding designed from metabolomics.
Authors: Korneli, Claudia; Bolten, Christoph Josef; Godard, Thibault; Franco-Lara, Ezequiel; Wittmann, Christoph
PaperIn vitro and in vivo RNA interference mediated suppression of Tn-caspase-1 for improved recombinant protein production in High Five cell culture with the baculovirus expression vector system.
Hebert, Colin G; Valdes, James J; Bentley, William E · 2009 · Biotechnology and bioengineering · DOI
Full title: In vitro and in vivo RNA interference mediated suppression of Tn-caspase-1 for improved recombinant protein production in High Five cell culture with the baculovirus expression vector system.
Authors: Hebert, Colin G; Valdes, James J; Bentley, William E
PaperEffect of modified glucose uptake using genetic engineering techniques on high-level recombinant protein production in escherichia coli dense cultures.
Chou, C H; Bennett, G N; San, K Y · 1994 · Biotechnology and bioengineering · DOI
Full title: Effect of modified glucose uptake using genetic engineering techniques on high-level recombinant protein production in escherichia coli dense cultures.
PaperMetabolic modeling to identify engineering targets for Komagataella phaffii: The effect of biomass composition on gene target identification.
Cankorur-Cetinkaya, Ayca; Dikicioglu, Duygu; Oliver, Stephen G · 2017 · Biotechnology and bioengineering · DOI
Full title: Metabolic modeling to identify engineering targets for Komagataella phaffii: The effect of biomass composition on gene target identification.
Authors: Cankorur-Cetinkaya, Ayca; Dikicioglu, Duygu; Oliver, Stephen G
PaperDecoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases.
Lemmerer, Martin; Mairhofer, Juergen; Lepak, Alexander et al. · 2019 · Biotechnology and bioengineering · DOI
Full title: Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases.
PaperDeregulation of methanol metabolism reverts transcriptional limitations of recombinant Pichia pastoris (Komagataella spp) with multiple expression cassettes under control of the AOX1 promoter.
Cámara, Elena; Monforte, Sergi; Albiol, Joan et al. · 2019 · Biotechnology and bioengineering · DOI
Full title: Deregulation of methanol metabolism reverts transcriptional limitations of recombinant Pichia pastoris (Komagataella spp) with multiple expression cassettes under control of the AOX1 promoter.
PaperApplication of green analytical chemistry to a green chemistry process: Magnetic resonance and Raman spectroscopic process monitoring of continuous ethanolic fermentation
Legner, Robin; Wirtz, Alexander; Koza, Tim et al. · 2019 · Biotechnology and Bioengineering · DOI
Full title: Application of green analytical chemistry to a green chemistry process: Magnetic resonance and Raman spectroscopic process monitoring of continuous ethanolic fermentation
Authors: Legner, Robin; Wirtz, Alexander; Koza, Tim; Tetzlaff, Till K.; Nickisch‐Hartfiel, Anna; Jaeger, Martin
PaperRecombinant protein production-associated metabolic burden reflects anabolic constraints and reveals similarities to a carbon overfeeding response.
Li, Zhaopeng; Rinas, Ursula · 2021 · Biotechnology and bioengineering · DOI
Full title: Recombinant protein production-associated metabolic burden reflects anabolic constraints and reveals similarities to a carbon overfeeding response.
PaperSuperior protein titers in half the fermentation time: Promoter and process engineering for the glucose-regulated GTH1 promoter of Pichia pastoris.
Prielhofer, Roland; Reichinger, Michaela; Wagner, Nina et al. · 2018 · Biotechnology and bioengineering · DOI
Full title: Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose-regulated GTH1 promoter of Pichia pastoris.
PaperEngineering of alcohol dehydrogenase 2 hybrid‐promoter architectures in Pichia pastoris to enhance recombinant protein expression on ethanol
Burcu Gündüz Ergün; Brigitte Gasser; D. Mattanovich et al. · 2019 · Biotechnology and Bioengineering · DOI
Full title: Engineering of alcohol dehydrogenase 2 hybrid‐promoter architectures in Pichia pastoris to enhance recombinant protein expression on ethanol
Authors: Burcu Gündüz Ergün; Brigitte Gasser; D. Mattanovich; P. Çalık
PaperDownregulation of T7 RNA polymerase transcription enhances pET-based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis.
Sun, Xiao-Man; Zhang, Zi-Xu; Wang, Ling-Ru et al. · 2021 · Biotechnology and bioengineering · DOI
Full title: Downregulation of T7 RNA polymerase transcription enhances pET-based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis.
PaperOverexpression of the mitochondrial pyruvate carrier reduces lactate production and increases recombinant protein productivity in CHO cells.
Bulté, Dubhe B; Palomares, Laura A; Parra, Carolina Gómez et al. · 2020 · Biotechnology and bioengineering · DOI
Full title: Overexpression of the mitochondrial pyruvate carrier reduces lactate production and increases recombinant protein productivity in CHO cells.
Authors: Bulté, Dubhe B; Palomares, Laura A; Parra, Carolina Gómez; Martínez, Juan Andrés; Contreras, Martha A; Noriega, Lilia G; Ramírez, Octavio T
PaperAll You Can Eat Yeast: Substituting Hexose Transporters With AtSWEET7 Alleviates Glucose Repression, Enabling Simultaneous Utilization of Sugars in Renewable Feedstocks.
Nurzhan Kuanyshev; Degaulle Dai; Jungyeon Kim et al. · 2026 · Biotechnology and Bioengineering · DOI
Full title: All You Can Eat Yeast: Substituting Hexose Transporters With AtSWEET7 Alleviates Glucose Repression, Enabling Simultaneous Utilization of Sugars in Renewable Feedstocks.
Authors: Nurzhan Kuanyshev; Degaulle Dai; Jungyeon Kim; Nam Kyu Kang; Ming-Hsun Cheng; Vijay Singh; Yong-Su Jin
PaperAerosol infectivity of a Baculovirus to Trichoplusia ni larvae: An alternative larval inoculation strategy for recombinant protein production.
Jinn, Tzyy-Rong; Kao, Suey-Sheng; Tseng, Yin-Chin et al. · 2009 · Biotechnology progress · DOI
Full title: Aerosol infectivity of a Baculovirus to Trichoplusia ni larvae: An alternative larval inoculation strategy for recombinant protein production.
PaperCharacterization and designing of an SAM riboswitch to establish a high‐throughput screening platform for SAM overproduction in Saccharomyces cerevisiae
Xiaomeng Fu; Xiaoru Zuo; Xiaomeng Zhao et al. · 2023 · Biotechnology and Bioengineering · DOI
Full title: Characterization and designing of an SAM riboswitch to establish a high‐throughput screening platform for SAM overproduction in Saccharomyces cerevisiae
PaperProcess Intensification for Recombinant Protein Production in E. coli via Identification of Active Nodes in Cellular Metabolism and Dynamic Flux Balance Analysis.
Dodia, Hardik; Muddana, Charandatta; Mishra, Vivek et al. · 2025 · Biotechnology and bioengineering · DOI
Full title: Process Intensification for Recombinant Protein Production in E. coli via Identification of Active Nodes in Cellular Metabolism and Dynamic Flux Balance Analysis.
PaperDynamic flux balance analysis of high cell density fed‐batch culture of Escherichia coli BL21 (DE3) with mass spectrometry‐based spent media analysis
Hardik Dodia; Vivek Mishra; Prajval Nakrani et al. · 2024 · Biotechnology and Bioengineering · DOI
Full title: Dynamic flux balance analysis of high cell density fed‐batch culture of Escherichia coli BL21 (DE3) with mass spectrometry‐based spent media analysis
Authors: Hardik Dodia; Vivek Mishra; Prajval Nakrani; Charandatta Muddana; A. Kedia; Sneha S. Rana; Deepti Sahasrabuddhe; P. Wangikar
PaperParallel steady state studies on a milliliter scale accelerate fed-batch bioprocess design for recombinant protein production with Escherichia coli.
Schmideder, Andreas; Cremer, Johannes H; Weuster-Botz, Dirk · 2016 · Biotechnology progress · DOI
Full title: Parallel steady state studies on a milliliter scale accelerate fed-batch bioprocess design for recombinant protein production with Escherichia coli.
Authors: Schmideder, Andreas; Cremer, Johannes H; Weuster-Botz, Dirk
PaperOnline measurement of the respiratory activity in shake flasks enables the identification of cultivation phases and patterns indicating recombinant protein production in various Escherichia coli host strains.
Ihling, Nina; Bittner, Natalie; Diederichs, Sylvia et al. · 2018 · Biotechnology progress · DOI
Full title: Online measurement of the respiratory activity in shake flasks enables the identification of cultivation phases and patterns indicating recombinant protein production in various Escherichia coli host strains.
PaperEffect of Photocaged Isopropyl β-d-1-thiogalactopyranoside Solubility on the Light Responsiveness of LacI-controlled Expression Systems in Different Bacteria.
Hogenkamp, Fabian; Hilgers, Fabienne; Knapp, Andreas et al. · 2021 · Chembiochem : a European journal of chemical biology · DOI
Full title: Effect of Photocaged Isopropyl β-d-1-thiogalactopyranoside Solubility on the Light Responsiveness of LacI-controlled Expression Systems in Different Bacteria.
PaperEffect of Glycosylation on the Biocatalytic Properties of Hydroxynitrile Lyase from the Passion Fruit, Passiflora edulis: A Comparison of Natural and Recombinant Enzymes.
Nuylert, Aem; Ishida, Yuko; Asano, Yasuhisa · 2017 · Chembiochem : a European journal of chemical biology · DOI
Full title: Effect of Glycosylation on the Biocatalytic Properties of Hydroxynitrile Lyase from the Passion Fruit, Passiflora edulis: A Comparison of Natural and Recombinant Enzymes.
PaperBioactive Compounds Derived From Natural Foods Against Metabolic Syndrome and the Advances of Resveratrol and Caffeic Acid in Microbial Cell Factory Production: A Review
Tao Li; Jingjing Shi; Tao Liu et al. · 2025 · Food Frontiers · DOI
Full title: Bioactive Compounds Derived From Natural Foods Against Metabolic Syndrome and the Advances of Resveratrol and Caffeic Acid in Microbial Cell Factory Production: A Review
Authors: Tao Li; Jingjing Shi; Tao Liu; Chongxiang Xue; Lin Han; Mingzhe Zhang; Linhua Zhao; Xiaolin Tong
PaperTransfer of disulfide bond formation modules via yeast artificial chromosomes promotes the expression of heterologous proteins in Kluyveromyces marxianus
Pingping Wu; Wenjuan Mo; Tian Tian et al. · 2023 · bioRxiv · DOI
Full title: Transfer of disulfide bond formation modules via yeast artificial chromosomes promotes the expression of heterologous proteins in Kluyveromyces marxianus
Authors: Pingping Wu; Wenjuan Mo; Tian Tian; Kunfeng Song; Yilin Lyu; Haiyan Ren; Jungang Zhou; Yao Yu; Hong Lu
PaperBiological Control of Wheat Powdery Mildew Disease by the Termite-associated Fungus Aspergillus chevalieri BYST01 and Potential Role of Secondary Metabolites.
Shuxiang Zhang; Zhongdi Huang; Huanhuan Xu et al. · 2023 · Pest Management Science · DOI
Full title: Biological Control of Wheat Powdery Mildew Disease by the Termite-associated Fungus Aspergillus chevalieri BYST01 and Potential Role of Secondary Metabolites.
PaperEffect of glucose supply strategy on acetate accumulation, growth, and recombinant protein production by Escherichia coli BL21 (λDE3) and Escherichia coli JM109
Joseph Shiloach; J. Kaufman; A. S. Guillard et al. · 2000 · Biotechnology and Bioengineering · DOI
Full title: Effect of glucose supply strategy on acetate accumulation, growth, and recombinant protein production by Escherichia coli BL21 (λDE3) and Escherichia coli JM109
Authors: Joseph Shiloach; J. Kaufman; A. S. Guillard; R. Fass
PaperA High Throughput Assay for Measuring Secreted Protein Based on a de novo Fluorescent Reporter Reveals Regulatory and Structural Insights in Salmonella Type Three Secretion System.
Leach, Samuel Alexander; Summers, Jordan Scott; Wen, Edward et al. · 2025 · bioRxiv : the preprint server for biology · DOI
Full title: A High Throughput Assay for Measuring Secreted Protein Based on a de novo Fluorescent Reporter Reveals Regulatory and Structural Insights in Salmonella Type Three Secretion System.
Authors: Leach, Samuel Alexander; Summers, Jordan Scott; Wen, Edward; Tullman-Ercek, Danielle
PaperIntracellular expression of Vitreoscilla hemoglobin modifies microaerobic Escherichia coli metabolism through elevated concentration and specific activity of cytochrome o.
Tsai, P S; Nägeli, M; Bailey, J E · 1996 · Biotechnology and bioengineering · DOI
Full title: Intracellular expression of Vitreoscilla hemoglobin modifies microaerobic Escherichia coli metabolism through elevated concentration and specific activity of cytochrome o.
PaperEscherichia coli strains with precise domain deletions in the ribonuclease RNase E can achieve greatly enhanced levels of membrane protein production.
Vasilopoulou, Eleni; Chroumpi, Tania; Skretas, Georgios · 2024 · Protein science : a publication of the Protein Society · DOI
Full title: Escherichia coli strains with precise domain deletions in the ribonuclease RNase E can achieve greatly enhanced levels of membrane protein production.
PaperProcess simulation for recombinant protein production: cost estimation and sensitivity analysis for heparinase I expressed in Escherichia coli.
Ernst, S; Garro, O A; Winkler, S et al. · 1997 · Biotechnology and bioengineering · DOI
Full title: Process simulation for recombinant protein production: cost estimation and sensitivity analysis for heparinase I expressed in Escherichia coli.
Authors: Ernst, S; Garro, O A; Winkler, S; Venkataraman, G; Langer, R; Cooney, C L; Sasisekharan, R
PaperInducing single-cell suspension of BTI-TN5B1-4 insect cells: I. The use of sulfated polyanions to prevent cell aggregation and enhance recombinant protein production.
Dee, K U; Shuler, M L; Wood, H A · 1997 · Biotechnology and bioengineering · DOI
Full title: Inducing single-cell suspension of BTI-TN5B1-4 insect cells: I. The use of sulfated polyanions to prevent cell aggregation and enhance recombinant protein production.
PaperA mathematical model of the trafficking of acid-dependent enveloped viruses: application to the binding, uptake, and nuclear accumulation of baculovirus.
Dee, K U; Shuler, M L · 1997 · Biotechnology and bioengineering · DOI
Full title: A mathematical model of the trafficking of acid-dependent enveloped viruses: application to the binding, uptake, and nuclear accumulation of baculovirus.
PaperUnderstanding the metabolic burden of recombinant antibody production in Saccharomyces cerevisiae using a quantitative metabolomics approach.
de Ruijter, Jorg C; Koskela, Essi V; Nandania, Jatin et al. · 2018 · Yeast (Chichester, England) · DOI
Full title: Understanding the metabolic burden of recombinant antibody production in Saccharomyces cerevisiae using a quantitative metabolomics approach.
Authors: de Ruijter, Jorg C; Koskela, Essi V; Nandania, Jatin; Frey, Alexander D; Velagapudi, Vidya
PaperMembrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures.
Kawamoto, Jun; Kurihara, Tatsuo · 2022 · Methods in molecular biology (Clifton, N.J.) · DOI
Full title: Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures.
Authors: Kawamoto, Jun; Kurihara, Tatsuo
Year: 2022
Venue: Methods in molecular biology (Clifton, N.J.)
PaperImproved yields of the extracellular domain of the epidermal growth factor receptor produced using the baculovirus expression system by medium replacement following infection.
Tom, R L; Debanne, M T; Bédard, C et al. · 1995 · Applied microbiology and biotechnology · DOI
Full title: Improved yields of the extracellular domain of the epidermal growth factor receptor produced using the baculovirus expression system by medium replacement following infection.
Authors: Tom, R L; Debanne, M T; Bédard, C; Caron, A W; Massie, B; Kamen, A A
PaperNovel helper factors influencing recombinant protein production in Pichia pastoris based on proteomic analysis under simulated microgravity.
Huangfu, Jie; Qi, Feng; Liu, Hongwei et al. · 2015 · Applied microbiology and biotechnology · DOI
Full title: Novel helper factors influencing recombinant protein production in Pichia pastoris based on proteomic analysis under simulated microgravity.
Authors: Huangfu, Jie; Qi, Feng; Liu, Hongwei; Zou, Hanfa; Ahmed, Muhammad Saad; Li, Chun
PaperA novel synthetic medium and expression system for subzero growth and recombinant protein production in Pseudoalteromonas haloplanktis TAC125.
Sannino, F; Giuliani, M; Salvatore, U et al. · 2017 · Applied microbiology and biotechnology · DOI
Full title: A novel synthetic medium and expression system for subzero growth and recombinant protein production in Pseudoalteromonas haloplanktis TAC125.
Authors: Sannino, F; Giuliani, M; Salvatore, U; Apuzzo, G A; de Pascale, D; Fani, R; Fondi, M; Marino, G; Tutino, M L; Parrilli, E
PaperIntegrating metabolic modeling and population heterogeneity analysis into optimizing recombinant protein production by Komagataella (Pichia) pastoris.
Theron, Chrispian W; Berrios, Julio; Delvigne, Frank et al. · 2018 · Applied microbiology and biotechnology · DOI
Full title: Integrating metabolic modeling and population heterogeneity analysis into optimizing recombinant protein production by Komagataella (Pichia) pastoris.
Authors: Theron, Chrispian W; Berrios, Julio; Delvigne, Frank; Fickers, Patrick
PaperDeciphering bacterial xylose metabolism and metabolic engineering of industrial microorganisms for use as efficient microbial cell factories.
Kim, Donghyuk; Woo, Han Min · 2018 · Applied microbiology and biotechnology · DOI
Full title: Deciphering bacterial xylose metabolism and metabolic engineering of industrial microorganisms for use as efficient microbial cell factories.
PaperIdentification of HUGT1 as a potential BiP activator and a cellular target for improvement of recombinant protein production using a cDNA screening system.
Ku, Sebastian Chih Yuan; Lwa, Teng Rhui; Giam, Maybelline et al. · 2009 · Molecules and cells · DOI
Full title: Identification of HUGT1 as a potential BiP activator and a cellular target for improvement of recombinant protein production using a cDNA screening system.
Authors: Ku, Sebastian Chih Yuan; Lwa, Teng Rhui; Giam, Maybelline; Yap, Miranda Gek Sim; Chao, Sheng-Hao
PaperOxygen transfer as a tool for fine-tuning recombinant protein production by Pichia pastoris under glyceraldehyde-3-phosphate dehydrogenase promoter.
Güneş, Hande; Çalık, Pınar · 2016 · Bioprocess and biosystems engineering · DOI
Full title: Oxygen transfer as a tool for fine-tuning recombinant protein production by Pichia pastoris under glyceraldehyde-3-phosphate dehydrogenase promoter.
PaperUse of a stress-minimisation paradigm in high cell density fed-batch Escherichia coli fermentations to optimise recombinant protein production.
Wyre, Chris; Overton, Tim W · 2014 · Journal of industrial microbiology & biotechnology · DOI
Full title: Use of a stress-minimisation paradigm in high cell density fed-batch Escherichia coli fermentations to optimise recombinant protein production.
Authors: Wyre, Chris; Overton, Tim W
Year: 2014
Venue: Journal of industrial microbiology & biotechnology
PaperA constitutive expression system for Pichia pastoris based on the PGK1 promoter.
Arruda, Andrelisse; Reis, Viviane Castelo Branco; Batista, Vinícius Daniel Ferreira et al. · 2016 · Biotechnology letters · DOI
Full title: A constitutive expression system for Pichia pastoris based on the PGK1 promoter.
Authors: Arruda, Andrelisse; Reis, Viviane Castelo Branco; Batista, Vinícius Daniel Ferreira; Daher, Bruno Sahim; Piva, Luiza Cesca; De Marco, Janice Lisboa; de Moraes, Lidia Maria Pepe; Torres, Fernando Araripe Gonçalves
PaperCorrelation Between Expression of Recombinant Proteins and Abundance of H3K4Me3 on the Enhancer of Human Cytomegalovirus Major Immediate-Early Promoter.
Soo, Benjamin P C; Tay, Julian; Ng, Shirelle et al. · 2017 · Molecular biotechnology · DOI
Full title: Correlation Between Expression of Recombinant Proteins and Abundance of H3K4Me3 on the Enhancer of Human Cytomegalovirus Major Immediate-Early Promoter.
Authors: Soo, Benjamin P C; Tay, Julian; Ng, Shirelle; Ho, Steven C L; Yang, Yuansheng; Chao, Sheng-Hao
PaperConstruction of a Kluyveromyces lactis ku80⁻ host strain for recombinant protein production: extracellular secretion of pectin lyase and a streptavidin-pectin lyase chimera.
Colombo, Lívia T; Rosa, Júlio César C; Bragança, Caio R S et al. · 2014 · Molecular biotechnology · DOI
Full title: Construction of a Kluyveromyces lactis ku80⁻ host strain for recombinant protein production: extracellular secretion of pectin lyase and a streptavidin-pectin lyase chimera.
Authors: Colombo, Lívia T; Rosa, Júlio César C; Bragança, Caio R S; Ignacchiti, Raphael P; Alvim, Mariana C T; Silveira, Wendel B; de Queiroz, Marisa V; Bazzolli, Denise M S; Passos, Flávia M L
PaperNovel chemometric strategy based on the application of artificial neural networks to crossed mixture design for the improvement of recombinant protein production in continuous culture.
Didier, Caroline; Forno, Guillermina; Etcheverrigaray, Marina et al. · 2009 · Analytica chimica acta · DOI
Full title: Novel chemometric strategy based on the application of artificial neural networks to crossed mixture design for the improvement of recombinant protein production in continuous culture.
PaperLight and heat-shock mediated TDA1 overexpression as a tool for controlled high-yield recombinant protein production in Chlamydomonas reinhardtii chloroplasts
Saskya E. Carrera-Pacheco; B. Hankamer; M. Oey · 2020 · DOI
Full title: Light and heat-shock mediated TDA1 overexpression as a tool for controlled high-yield recombinant protein production in Chlamydomonas reinhardtii chloroplasts
Authors: Saskya E. Carrera-Pacheco; B. Hankamer; M. Oey
PaperEfficient 2-O-α-D-glucopyranosyl-sn-glycerol production by single whole-cell biotransformation through combined engineering and expression regulation with novel sucrose phosphorylase from Leuconostoc mesenteroides ATCC 8293.
Peifeng Duan; Mengfei Long; Xian Zhang et al. · 2023 · Bioresource Technology · DOI
Full title: Efficient 2-O-α-D-glucopyranosyl-sn-glycerol production by single whole-cell biotransformation through combined engineering and expression regulation with novel sucrose phosphorylase from Leuconostoc mesenteroides ATCC 8293.
PaperEngineering of Corynebacterium glutamicum for para-coumaric acid biosynthesis from lignocellulosic biomass.
Do-Wook Kim; Sang Min Kim; Imam Hidayat Nurwahid et al. · 2025 · Bioresource Technology · DOI
Full title: Engineering of Corynebacterium glutamicum for para-coumaric acid biosynthesis from lignocellulosic biomass.
Authors: Do-Wook Kim; Sang Min Kim; Imam Hidayat Nurwahid; Chang Soo Kim; Ji-Sun Lee; Sung Wook Eun; Changmin Sung; Gyeongtaek Gong; J. Ko; Y. Um; Sung Ok Han; Jung Ho Ahn
PaperHeterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt.
den Haan, Riaan; Rose, Shaunita H; Cripwell, Rosemary A et al. · 2021 · Biotechnology advances · DOI
Full title: Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt.
Authors: den Haan, Riaan; Rose, Shaunita H; Cripwell, Rosemary A; Trollope, Kim M; Myburgh, Marthinus W; Viljoen-Bloom, Marinda; van Zyl, Willem H
PaperBiosynthesis of functional carbohydrates from glycolytic node precursors glucose-6-phosphate and fructose-6-phosphate: Advances and prospects.
Ran You; Xueqin Lv; Yan Zhang et al. · 2026 · Biotechnology Advances · DOI
Full title: Biosynthesis of functional carbohydrates from glycolytic node precursors glucose-6-phosphate and fructose-6-phosphate: Advances and prospects.
Authors: Ran You; Xueqin Lv; Yan Zhang; Jian Chen; Long Liu
PaperMolecular background of HAC1-driven improvement in the secretion of recombinant protein in Yarrowia lipolytica based on comparative transcriptomics.
Full title: Molecular background of HAC1-driven improvement in the secretion of recombinant protein in Yarrowia lipolytica based on comparative transcriptomics.
PaperExploring the potential of the glycerol-3-phosphate dehydrogenase 2 (GPD2) promoter for recombinant gene expression in Saccharomyces cerevisiae.
Knudsen, Jan Dines; Johanson, Ted; Eliasson Lantz, Anna et al. · 2015 · Biotechnology reports (Amsterdam, Netherlands) · DOI
Full title: Exploring the potential of the glycerol-3-phosphate dehydrogenase 2 (GPD2) promoter for recombinant gene expression in Saccharomyces cerevisiae.
Authors: Knudsen, Jan Dines; Johanson, Ted; Eliasson Lantz, Anna; Carlquist, Magnus
PaperPERISCOPE-Opt: Machine learning-based prediction of optimal fermentation conditions and yields of recombinant periplasmic protein expressed in Escherichia coli.
Packiam, Kulandai Arockia Rajesh; Ooi, Chien Wei; Li, Fuyi et al. · 2022 · Computational and structural biotechnology journal · DOI
Full title: PERISCOPE-Opt: Machine learning-based prediction of optimal fermentation conditions and yields of recombinant periplasmic protein expressed in Escherichia coli.
PaperApplication of new approach methods (NAMs) in food safety with considerations for new proteins.
Hanlon, Paul R; Hepburn, Paul A; Scimeca, Joseph A et al. · 2026 · Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · DOI
Full title: Application of new approach methods (NAMs) in food safety with considerations for new proteins.
Authors: Hanlon, Paul R; Hepburn, Paul A; Scimeca, Joseph A; Schaneberg, Brian T
Year: 2026
Venue: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
PaperSafety evaluation of honey truffle sweet protein produced from Komagataellaphaffii.
Potter, Susan M; Zhao, Dan; Roberts, Ashley et al. · 2026 · Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · DOI
Full title: Safety evaluation of honey truffle sweet protein produced from Komagataellaphaffii.
Authors: Potter, Susan M; Zhao, Dan; Roberts, Ashley; Peterson, Robert; Connors, Daniel E; Mayfield, David B; Lloyd, Mel; Martin, Thomas; Papineni, Sabitha; Tosi, Giulia
Year: 2026
Venue: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
PaperUnveiling the mastery of bacterial navigation: The role of methyl-accepting chemotactic proteins in sensing and mobility through environments and their promising applications in food sustainability.
Li, Jiaxun; Bekhit, Salma A; Zhou, Guicheng et al. · 2025 · Food research international (Ottawa, Ont.) · DOI
Full title: Unveiling the mastery of bacterial navigation: The role of methyl-accepting chemotactic proteins in sensing and mobility through environments and their promising applications in food sustainability.
PaperProduction of bovine beta-lactoglobulin and hen egg ovalbumin by Trichoderma reesei using precision fermentation technology and testing of their techno-functional properties.
Aro, Nina; Ercili-Cura, Dilek; Andberg, Martina et al. · 2023 · Food research international (Ottawa, Ont.) · DOI
Full title: Production of bovine beta-lactoglobulin and hen egg ovalbumin by Trichoderma reesei using precision fermentation technology and testing of their techno-functional properties.
Authors: Aro, Nina; Ercili-Cura, Dilek; Andberg, Martina; Silventoinen, Pia; Lille, Martina; Hosia, Waltteri; Nordlund, Emilia; Landowski, Christopher P
PaperBlue food proteins-derived immunomodulatory peptides: sources, preparation, identification techniques, mechanisms, and critical challenges of commercial application.
Wu, Yu-Han; Chen, Yan; Zhang, Ke-Lin et al. · 2026 · Food research international (Ottawa, Ont.) · DOI
Full title: Blue food proteins-derived immunomodulatory peptides: sources, preparation, identification techniques, mechanisms, and critical challenges of commercial application.
PaperIsolation of an invertebrate-type lysozyme from the nephridia of the echiura, Urechis unicinctus, and its recombinant production and activities.
Oh, Hye Young; Kim, Chan-Hee; Go, Hye-Jin et al. · 2018 · Fish & shellfish immunology · DOI
Full title: Isolation of an invertebrate-type lysozyme from the nephridia of the echiura, Urechis unicinctus, and its recombinant production and activities.
Authors: Oh, Hye Young; Kim, Chan-Hee; Go, Hye-Jin; Park, Nam Gyu
PaperCasein micelle formation as a calcium phosphate phase separation process: Preparation of artificial casein micelles through vacuum evaporation and membrane processes
Antuma, Laurens J.; Stadler, Maybritt; Garamus, Vasil M. et al. · 2024 · Innovative Food Science & Emerging Technologies · DOI
Full title: Casein micelle formation as a calcium phosphate phase separation process: Preparation of artificial casein micelles through vacuum evaporation and membrane processes
Authors: Antuma, Laurens J.; Stadler, Maybritt; Garamus, Vasil M.; Boom, Remko M.; Keppler, Julia K.
PaperEnhancement in the production of recombinant human paraoxonase 1 in Escherichia coli: A comprehensive approach of cellular engineering and optimization of protein folding process in vitro.
Pal, Ankit; Chaudhuri, Tapan K · 2022 · International journal of biological macromolecules · DOI
Full title: Enhancement in the production of recombinant human paraoxonase 1 in Escherichia coli: A comprehensive approach of cellular engineering and optimization of protein folding process in vitro.
Authors: Pal, Ankit; Chaudhuri, Tapan K
Year: 2022
Venue: International journal of biological macromolecules
PaperBoosting extracellular FastPETase production in E. coli: A combined approach of cognate chaperones co-expression and vesicle nucleating peptide tag fusion.
Wu, Ting; Sun, Huashan; Wang, Wenyao et al. · 2024 · International journal of biological macromolecules · DOI
Full title: Boosting extracellular FastPETase production in E. coli: A combined approach of cognate chaperones co-expression and vesicle nucleating peptide tag fusion.
PaperControlling the carbon flux between glycolysis and the pentose phosphate pathway via targeted protein degradation in Corynebacterium glutamicum.
Norihiko Takemoto; Wenhui Hao; Hiroki Matsuzawa et al. · 2025 · Journal of Bioscience and Bioengineering · DOI
Full title: Controlling the carbon flux between glycolysis and the pentose phosphate pathway via targeted protein degradation in Corynebacterium glutamicum.
PaperEnhancing intracellular NADPH bioavailability through improving pentose phosphate pathway flux and its application in biocatalysis asymmetric reduction reaction.
Lin Yuan; Yanting Qin; Z. Zou et al. · 2022 · Journal of Bioscience and Bioengineering · DOI
Full title: Enhancing intracellular NADPH bioavailability through improving pentose phosphate pathway flux and its application in biocatalysis asymmetric reduction reaction.
Authors: Lin Yuan; Yanting Qin; Z. Zou; Bright Appiah; Hao Huang; Zhong-Hua Yang; Can Qun
PaperMamestra brassicae NIAS-Mb-32 cell strain 2g2 enables high-yield recombinant protein production in baculovirus-free and baculovirus-based insect cell expression.
Yamamoto, Yotaro; Kajiura, Hiroyuki; Nishibu, Takahiro et al. · 2022 · Journal of bioscience and bioengineering · DOI
Full title: Mamestra brassicae NIAS-Mb-32 cell strain 2g2 enables high-yield recombinant protein production in baculovirus-free and baculovirus-based insect cell expression.
PaperCombined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures.
Cos, Oriol; Serrano, Alicia; Montesinos, José Luis et al. · 2005 · Journal of biotechnology · DOI
Full title: Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures.
Authors: Cos, Oriol; Serrano, Alicia; Montesinos, José Luis; Ferrer, Pau; Cregg, James M; Valero, Francisco
PaperImproving the batch-to-batch reproducibility of microbial cultures during recombinant protein production by regulation of the total carbon dioxide production.
Jenzsch, Marco; Gnoth, Stefan; Kleinschmidt, Martin et al. · 2007 · Journal of biotechnology · DOI
Full title: Improving the batch-to-batch reproducibility of microbial cultures during recombinant protein production by regulation of the total carbon dioxide production.
PaperImproved recombinant protein production in Aspergillus oryzae lacking both α-1,3-glucan and galactosaminogalactan in batch culture with a lab-scale bioreactor.
Ichikawa, Hikaru; Miyazawa, Ken; Komeiji, Keisuke et al. · 2022 · Journal of bioscience and bioengineering · DOI
Full title: Improved recombinant protein production in Aspergillus oryzae lacking both α-1,3-glucan and galactosaminogalactan in batch culture with a lab-scale bioreactor.
PaperUse of a mixture of glucose and methanol as substrates for the production of recombinant trypsinogen in continuous cultures with Pichia pastoris Mut+.
L. Paulova; P. Hyka; B. Branská et al. · 2012 · Journal of Biotechnology · DOI
Full title: Use of a mixture of glucose and methanol as substrates for the production of recombinant trypsinogen in continuous cultures with Pichia pastoris Mut+.
Authors: L. Paulova; P. Hyka; B. Branská; K. Melzoch; K. Kovar
PaperEnhancing thermo-induced recombinant protein production in Escherichia coli by temperature oscillations and post-induction nutrient feeding strategies.
Caspeta, Luis; Lara, Alvaro R; Pérez, Néstor O et al. · 2013 · Journal of biotechnology · DOI
Full title: Enhancing thermo-induced recombinant protein production in Escherichia coli by temperature oscillations and post-induction nutrient feeding strategies.
PaperHigh yield production of the latex clearing protein from Gordonia polyisoprenivorans VH2 in fed batch fermentations using a recombinant strain of Escherichia coli.
Altenhoff, Anna-Lena; Thierbach, Sven; Steinbüchel, Alexander · 2020 · Journal of biotechnology · DOI
Full title: High yield production of the latex clearing protein from Gordonia polyisoprenivorans VH2 in fed batch fermentations using a recombinant strain of Escherichia coli.
Authors: Altenhoff, Anna-Lena; Thierbach, Sven; Steinbüchel, Alexander
PaperSubstitution of the 5'-untranslated region in the alcohol oxidase 1 promoter of Komagataella phaffii alleviated the repression in the presence of glycerol.
Ziwei Zhou; Aibo Feng; Wenjie Cong et al. · 2025 · Journal of Biotechnology · DOI
Full title: Substitution of the 5'-untranslated region in the alcohol oxidase 1 promoter of Komagataella phaffii alleviated the repression in the presence of glycerol.
PaperTransfection of miR-31* boosts oxidative phosphorylation metabolism in the mitochondria and enhances recombinant protein production in Chinese hamster ovary cells.
Martinez-Lopez, Jesus E; Coleman, Orla; Meleady, Paula et al. · 2021 · Journal of biotechnology · DOI
Full title: Transfection of miR-31* boosts oxidative phosphorylation metabolism in the mitochondria and enhances recombinant protein production in Chinese hamster ovary cells.
Authors: Martinez-Lopez, Jesus E; Coleman, Orla; Meleady, Paula; Clynes, Martin
PaperFungal fermentation inducing improved nutritional qualities associated with altered secondary protein structure of soybean meal determined by FTIR spectroscopy
Full title: Fungal fermentation inducing improved nutritional qualities associated with altered secondary protein structure of soybean meal determined by FTIR spectroscopy
PaperRice GABA®: Valorization of rice germ through lactic acid bacterial precision fermentation for enhanced GABA production and functional properties
Hyun, Jimin; Rheu, Kyoung-Min; Lee, Seokmin et al. · 2025 · LWT · DOI
Full title: Rice GABA®: Valorization of rice germ through lactic acid bacterial precision fermentation for enhanced GABA production and functional properties
PaperRecent trends in the production of proteins by Precision Fermentation for improving the Quality and Attributes of Food: Role of Genetic engineering towards next generation of food production
Srutee Rout; P. Srivastav · 2025 · The Microbe · DOI
Full title: Recent trends in the production of proteins by Precision Fermentation for improving the Quality and Attributes of Food: Role of Genetic engineering towards next generation of food production
PaperA stress-tolerant, chaperone-like peptidylprolyl isomerase (PPIase) from Chromohalobacter salexigens as a fusion partner for soluble, correctly folded expression in Escherichia coli.
Azumi Nagaoka; F. Yamagami; Yuto Otsuka et al. · 2026 · Journal of Biotechnology · DOI
Full title: A stress-tolerant, chaperone-like peptidylprolyl isomerase (PPIase) from Chromohalobacter salexigens as a fusion partner for soluble, correctly folded expression in Escherichia coli.
Authors: Azumi Nagaoka; F. Yamagami; Yuto Otsuka; Madoka Kashiki; Ayaka Kikuchi; Yako Osaki; Hirohito Tsurumaru; M. Tokunaga; M. Ishibashi
PaperDevelopment of a dedicated Golden Gate Assembly Platform (RtGGA) for Rhodotorula toruloides
Nemailla Bonturi; M. J. Pinheiro; Paola Monteiro de Oliveira et al. · 2022 · bioRxiv · DOI
Full title: Development of a dedicated Golden Gate Assembly Platform (RtGGA) for Rhodotorula toruloides
Authors: Nemailla Bonturi; M. J. Pinheiro; Paola Monteiro de Oliveira; Eka Rusadze; T. Eichinger; Gintarė Liudžiūtė; Juliano Sabedotti De Biaggi; Age Brauer; M. Remm; E. A. Miranda; R. Ledesma‐Amaro; Petri-Jaan Lahtvee
PaperRational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagataella phaffii) using the methanol-free GAP promoter. Where do we stand?
García-Ortega, Xavier; Cámara, Elena; Ferrer, Pau et al. · 2019 · New biotechnology · DOI
Full title: Rational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagataella phaffii) using the methanol-free GAP promoter. Where do we stand?
Authors: García-Ortega, Xavier; Cámara, Elena; Ferrer, Pau; Albiol, Joan; Montesinos-Seguí, José Luis; Valero, Francisco
PaperAssessing the feasibility of CRISPRa approaches to enhance protein-based biomaterial expression in bacterial systems for more efficient production.
Rodríguez-Alonso, Pablo; Chaskovska, Viktoriya; Venegas-Bustos, Desiré et al. · 2025 · Materials today. Bio · DOI
Full title: Assessing the feasibility of CRISPRa approaches to enhance protein-based biomaterial expression in bacterial systems for more efficient production.
Authors: Rodríguez-Alonso, Pablo; Chaskovska, Viktoriya; Venegas-Bustos, Desiré; Herraiz, Alba; Alonso, Matilde; Rodríguez-Cabello, Jose Carlos
PaperIncreased accumulation of fatty acids in engineered Saccharomyces cerevisiae by co-overexpression of interorganelle tethering protein and lipases.
Guoli Wang; Mingkai Li; Mengyu Ma et al. · 2024 · New Biotechnology · DOI
Full title: Increased accumulation of fatty acids in engineered Saccharomyces cerevisiae by co-overexpression of interorganelle tethering protein and lipases.
PaperKnock-out of the major regulator Flo8 in Komagataella phaffii results in unique host strain performance for methanol-free recombinant protein production.
Rebnegger, Corinna; Flores-Villegas, Mirelle; Kowarz, Viktoria et al. · 2024 · New biotechnology · DOI
Full title: Knock-out of the major regulator Flo8 in Komagataella phaffii results in unique host strain performance for methanol-free recombinant protein production.
PaperStudies of multifunctional DNA polymerase I from the extremely radiation resistant Deinococcus radiodurans: Recombinant expression, purification and characterization of the full-length protein and its large fragment.
Fernandes, A; Piotrowski, Y; Williamson, A et al. · 2021 · Protein expression and purification · DOI
Full title: Studies of multifunctional DNA polymerase I from the extremely radiation resistant Deinococcus radiodurans: Recombinant expression, purification and characterization of the full-length protein and its large fragment.
PaperDetection and optimization of microbial expression systems for extracellular production and purification of Ca2+-responsive phase-changing annexin fusions.
Li, Jinjing; Wu, Baokang; Ji, Yiting et al. · 2025 · Protein expression and purification · DOI
Full title: Detection and optimization of microbial expression systems for extracellular production and purification of Ca2+-responsive phase-changing annexin fusions.
Authors: Li, Jinjing; Wu, Baokang; Ji, Yiting; Zhang, Shuncheng; Ge, Yuanyuan; Fan, Jun
PaperComparison of the Efficacy of Cell Wall Disruption Methods for Saccharomyces cerevisiae INVSc1 cells Expressing Toxoplasma gondii Recombinant ROP6 Protein.
Karakavuk, Tuğba; Karakavuk, Muhammet; Gül, Ceren et al. · 2026 · Protein expression and purification · DOI
Full title: Comparison of the Efficacy of Cell Wall Disruption Methods for Saccharomyces cerevisiae INVSc1 cells Expressing Toxoplasma gondii Recombinant ROP6 Protein.
PaperComparative analysis of the Squamosa Promoter Binding-Like (SPL) gene family in Nicotiana benthamiana and Nicotiana tabacum.
Carmine de Paola; Victor Garcia-Carpintero; M. Vázquez-Vilar et al. · 2023 · Plant Science · DOI
Full title: Comparative analysis of the Squamosa Promoter Binding-Like (SPL) gene family in Nicotiana benthamiana and Nicotiana tabacum.
Authors: Carmine de Paola; Victor Garcia-Carpintero; M. Vázquez-Vilar; K. Kaminski; Asun Fernández-del-Carmen; N. Sierro; N. Ivanov; G. Giuliano; Peter Waterhouse; D. Orzáez
PaperThe radish defensins RsAFP1 and RsAFP2 act synergistically with caspofungin against Candida albicans biofilms.
Vriens, Kim; Cools, Tanne L; Harvey, Peta J et al. · 2016 · Peptides · DOI
Full title: The radish defensins RsAFP1 and RsAFP2 act synergistically with caspofungin against Candida albicans biofilms.
Authors: Vriens, Kim; Cools, Tanne L; Harvey, Peta J; Craik, David J; Braem, Annabel; Vleugels, Jozef; De Coninck, Barbara; Cammue, Bruno P A; Thevissen, Karin
PaperHigh-volume shake flask cultures as an alternative to cellbag technology for recombinant protein production in the baculovirus expression vector system (BEVS).
Cronin, Ciarán N · 2020 · Protein expression and purification · DOI
Full title: High-volume shake flask cultures as an alternative to cellbag technology for recombinant protein production in the baculovirus expression vector system (BEVS).
PaperEngineering an optimized expression operating unit for improved recombinant protein production in Escherichia coli.
Santos, Sara P O; Garcés, Luis Fabian S; Silva, Filipe S R et al. · 2022 · Protein expression and purification · DOI
Full title: Engineering an optimized expression operating unit for improved recombinant protein production in Escherichia coli.
Authors: Santos, Sara P O; Garcés, Luis Fabian S; Silva, Filipe S R; Santiago, Leonardo F; Pinheiro, Carina S; Alcantara-Neves, Neuza M; Pacheco, Luis G C
PaperDigital innovation integration into biotechnology for development of sustainable protein frontiers for poultry nutrition in a circular bioeconomy.
Full title: Digital innovation integration into biotechnology for development of sustainable protein frontiers for poultry nutrition in a circular bioeconomy.
PaperA new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactis
Reyhaneh Sadat Moosavi-Kohnehsari; Mahnaz Jafari-Sohi; Tohid Piri-Gharaghie et al. · 2025 · Poultry Science · DOI
Full title: A new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactis
PaperA CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli
Ming Zhao; Yuting Li; Fengqing Wang et al. · 2022 · Synthetic and Systems Biotechnology · DOI
Full title: A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli
PaperOptimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies.
Basiony, Mostafa; Ouyang, Liming; Wang, Danni et al. · 2022 · Synthetic and systems biotechnology · DOI
Full title: Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies.
PaperConstruction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories.
Wu, Yalun; Yuan, Qianqian; Yang, Yongfu et al. · 2023 · Synthetic and systems biotechnology · DOI
Full title: Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories.
PaperEnhancing delta-tocotrienol production in Saccharomyces cerevisiae via metabolic engineering strategies in conjunction with the mutagenesis of tocopherol cyclase.
Liu, Ziming; Tang, Min; Zhang, Wanze et al. · 2026 · Synthetic and systems biotechnology · DOI
Full title: Enhancing delta-tocotrienol production in Saccharomyces cerevisiae via metabolic engineering strategies in conjunction with the mutagenesis of tocopherol cyclase.
PaperEngineering ethanologenic bacterium Zymomonas mobilis for economic cellulosic bioethanol production using agro-industrial waste corncob residue
Yongfu Yang; Chenyue Zhang; Binan Geng et al. · 2026 · Synthetic and Systems Biotechnology · DOI
Full title: Engineering ethanologenic bacterium Zymomonas mobilis for economic cellulosic bioethanol production using agro-industrial waste corncob residue
Authors: Yongfu Yang; Chenyue Zhang; Binan Geng; Yi Tang; Haixia Yi; Jun Du; Qiang Fei; Shihui Yang
PaperStructural deviations between native and precision fermentation-derived food proteins: Techno-functionality and structure formation strategies
Keppler, J.K.; Werten, M.W.T.; Boom, R.M. · 2025 · Trends in Food Science & Technology · DOI
Full title: Structural deviations between native and precision fermentation-derived food proteins: Techno-functionality and structure formation strategies
PaperReplacement of the glucose phosphotransferase transport system by galactose permease reduces acetate accumulation and improves process performance of Escherichia coli for recombinant protein production without impairment of growth rate.
De Anda, Ramón; Lara, Alvaro R; Hernández, Vanessa et al. · 2006 · Metabolic engineering · DOI
Full title: Replacement of the glucose phosphotransferase transport system by galactose permease reduces acetate accumulation and improves process performance of Escherichia coli for recombinant protein production without impairment of growth rate.
Authors: De Anda, Ramón; Lara, Alvaro R; Hernández, Vanessa; Hernández-Montalvo, Verónica; Gosset, Guillermo; Bolívar, Francisco; Ramírez, Octavio T
PaperHigh-throughput screening for high-efficiency small-molecule biosynthesis
Rienzo, Matthew; Jackson, Shaina J.; Chao, Lawrence K. et al. · 2020 · Metabolic Engineering · DOI
Full title: High-throughput screening for high-efficiency small-molecule biosynthesis
Authors: Rienzo, Matthew; Jackson, Shaina J.; Chao, Lawrence K.; Leaf, Timothy; Schmidt, Thomas J.; Navidi, Adam H.; Nadler, Dana C.; Ohler, Maud; Leavell, Michael D.
PaperGoing beyond the limit: Increasing global translation activity leads to increased productivity of recombinant secreted proteins in Pichia pastoris.
Staudacher, Jennifer; Rebnegger, Corinna; Dohnal, Thomas et al. · 2022 · Metabolic engineering · DOI
Full title: Going beyond the limit: Increasing global translation activity leads to increased productivity of recombinant secreted proteins in Pichia pastoris.
PaperIdentification of novel metabolic engineering targets for S-adenosyl-L-Methionine production in Saccharomyces cerevisiae via genome-scale engineering.
Chang Dong; Carl Schultz; Wei Liu et al. · 2021 · Metabolic Engineering · DOI
Full title: Identification of novel metabolic engineering targets for S-adenosyl-L-Methionine production in Saccharomyces cerevisiae via genome-scale engineering.
Authors: Chang Dong; Carl Schultz; Wei Liu; J. Lian; Lei Huang; Zhinan Xu; Huimin Zhao
PaperSuppressors of amyloid-β toxicity improve recombinant protein production in yeast by reducing oxidative stress and tuning cellular metabolism.
Chen, Xin; Li, Xiaowei; Ji, Boyang et al. · 2022 · Metabolic engineering · DOI
Full title: Suppressors of amyloid-β toxicity improve recombinant protein production in yeast by reducing oxidative stress and tuning cellular metabolism.
Authors: Chen, Xin; Li, Xiaowei; Ji, Boyang; Wang, Yanyan; Ishchuk, Olena P; Vorontsov, Egor; Petranovic, Dina; Siewers, Verena; Engqvist, Martin K M
PaperBeyond CEN.PK - parallel engineering of selected S. cerevisiae strains reveals that superior chassis strains require different engineering approaches for limonene production.
Zhu, Yanmei; Yogiswara, Sasha; Willekens, Anke et al. · 2025 · Metabolic engineering · DOI
Full title: Beyond CEN.PK - parallel engineering of selected S. cerevisiae strains reveals that superior chassis strains require different engineering approaches for limonene production.
PaperHuman secretory signal peptide description by hidden Markov model and generation of a strong artificial signal peptide for secreted protein expression.
Barash, Steve; Wang, Wei; Shi, Yanggu · 2002 · Biochemical and biophysical research communications · DOI
Full title: Human secretory signal peptide description by hidden Markov model and generation of a strong artificial signal peptide for secreted protein expression.
Authors: Barash, Steve; Wang, Wei; Shi, Yanggu
Year: 2002
Venue: Biochemical and biophysical research communications
PaperDevelopment and Validation of a Liquid Chromatography-Mass Spectrometry Assay for the Quantitation of IPTG in <i>E. Coli</i> Fed-Batch Cultures
Fernández‐Castané, Alfred; Ruiz, Jordi; Caminal, Glòria et al. · 2010 · Analytical Chemistry · DOI
Full title: Development and Validation of a Liquid Chromatography-Mass Spectrometry Assay for the Quantitation of IPTG in <i>E. Coli</i> Fed-Batch Cultures
PaperMonitoring Phenotype Heterogeneity at the Single-Cell Level within Bacillus Populations Producing Poly-3-hydroxybutyrate by Label-Free Super-resolution Infrared Imaging
Cassio Lima; H. Muhamadali; R. Goodacre · 2023 · Analytical Chemistry · DOI
Full title: Monitoring Phenotype Heterogeneity at the Single-Cell Level within Bacillus Populations Producing Poly-3-hydroxybutyrate by Label-Free Super-resolution Infrared Imaging
PaperEngineering the Phenylpropanoid Pathway in <i>Rhodosporidium toruloides</i> for Naringenin Production from Tyrosine by Leveraging on its Native <i>PAL</i> Gene
Lee, Jaslyn Jie Lin; Ng, Kuan Rei; Liang, Jiaqi et al. · 2022 · ACS Food Science & Technology · DOI
Full title: Engineering the Phenylpropanoid Pathway in <i>Rhodosporidium toruloides</i> for Naringenin Production from Tyrosine by Leveraging on its Native <i>PAL</i> Gene
Authors: Lee, Jaslyn Jie Lin; Ng, Kuan Rei; Liang, Jiaqi; Cui, Xi; Li, Aaron Zongwei; Chen, Wei Ning
PaperLiquid Chromatography/Tandem Mass Spectrometry of Glycolytic Intermediates: Deconvolution of Coeluting Structural Isomers Based on Unique Product Ion Ratios
Ross, Keri Lyn; Dalluge, Joseph J. · 2009 · Analytical Chemistry · DOI
Full title: Liquid Chromatography/Tandem Mass Spectrometry of Glycolytic Intermediates: Deconvolution of Coeluting Structural Isomers Based on Unique Product Ion Ratios
PaperConstruction of 5-Aminolevulinic Acid Microbial Cell Factories through Identification of Novel Synthases and Metabolic Pathway Screens and Transporters.
Wang, Wenqiu; Xiang, Yulong; Yin, Guobin et al. · 2024 · Journal of agricultural and food chemistry · DOI
Full title: Construction of 5-Aminolevulinic Acid Microbial Cell Factories through Identification of Novel Synthases and Metabolic Pathway Screens and Transporters.
Authors: Wang, Wenqiu; Xiang, Yulong; Yin, Guobin; Hu, Shan; Cheng, Jian; Chen, Jian; Du, Guocheng; Kang, Zhen; Wang, Yang
PaperQuantitative Isotope-Dilution High-Resolution-Mass-Spectrometry Analysis of Multiple Intracellular Metabolites in <i>Clostridium autoethanogenum</i> with Uniformly <sup>13</sup>C-Labeled Standards Derived from Spirulina
Schatschneider, Sarah; Abdelrazig, Salah; Safo, Laudina et al. · 2018 · Analytical Chemistry · DOI
Full title: Quantitative Isotope-Dilution High-Resolution-Mass-Spectrometry Analysis of Multiple Intracellular Metabolites in <i>Clostridium autoethanogenum</i> with Uniformly <sup>13</sup>C-Labeled Standards Derived from Spirulina
Authors: Schatschneider, Sarah; Abdelrazig, Salah; Safo, Laudina; Henstra, Anne M.; Millat, Thomas; Kim, Dong‐Hyun; Winzer, Klaus; Minton, Nigel P.; Barrett, David A.
PaperDiscovery, Expression, and In Silico Safety Evaluation of Honey Truffle Sweetener, a Sweet Protein Derived from Mattirolomyces terfezioides and Produced by Heterologous Expression in Komagataella phaffii.
McFarland, Chase; Alkotaini, Bassam; Cowen, Chloe P et al. · 2024 · Journal of agricultural and food chemistry · DOI
Full title: Discovery, Expression, and In Silico Safety Evaluation of Honey Truffle Sweetener, a Sweet Protein Derived from Mattirolomyces terfezioides and Produced by Heterologous Expression in Komagataella phaffii.
Authors: McFarland, Chase; Alkotaini, Bassam; Cowen, Chloe P; Edwards, Michael G; Grein, Elizabeth; Hahn, Alan D; Jennings, Justine C; Patnaik, Ranjan; Potter, Susan M; Rael, Leonard T; Sharkey, Brendan P; Taylor, Steve L; Totman, Ryan; Van Simaeys, Karli; Vo, Phillip; Zhao, Dan; Connors, Daniel E
PaperBioconversion of Mushroom Chitin-Rich Waste into Valuable Chitin Oligosaccharides Using a Combined Approach of Biocatalysis and Precision Fermentation.
Windels, Alex; Declerck, Luna; Snoeck, Sofie et al. · 2025 · Journal of agricultural and food chemistry · DOI
Full title: Bioconversion of Mushroom Chitin-Rich Waste into Valuable Chitin Oligosaccharides Using a Combined Approach of Biocatalysis and Precision Fermentation.
Authors: Windels, Alex; Declerck, Luna; Snoeck, Sofie; Demeester, Wouter; Guidi, Chiara; Desmet, Tom; De Mey, Marjan
PaperMultisite Mutation of the Escherichia coli cAMP Receptor Protein: Enhancing Xylitol Biosynthesis by Activating Xylose Catabolism and Improving Strain Tolerance.
Yuan, Dongxu; Liu, Bingbing; Yuan, Xinsong et al. · 2023 · Journal of agricultural and food chemistry · DOI
Full title: Multisite Mutation of the Escherichia coli cAMP Receptor Protein: Enhancing Xylitol Biosynthesis by Activating Xylose Catabolism and Improving Strain Tolerance.
PaperBioconversion of Mushroom Chitin-Rich Waste into Valuable Chitin Oligosaccharides Using a Combined Approach of Biocatalysis and Precision Fermentation.
Windels, Alex; Declerck, Luna; Snoeck, Sofie et al. · 2025 · Journal of agricultural and food chemistry · DOI
Full title: Bioconversion of Mushroom Chitin-Rich Waste into Valuable Chitin Oligosaccharides Using a Combined Approach of Biocatalysis and Precision Fermentation.
Authors: Windels, Alex; Declerck, Luna; Snoeck, Sofie; Demeester, Wouter; Guidi, Chiara; Desmet, Tom; De Mey, Marjan
PaperDetermination of Nucleotide Sequences within Promoter Regions Affecting Promoter Compatibility between Zymomonas mobilis and Escherichia coli.
Haoyue Song; Yongfu Yang; Han Li et al. · 2022 · ACS Synthetic Biology · DOI
Full title: Determination of Nucleotide Sequences within Promoter Regions Affecting Promoter Compatibility between Zymomonas mobilis and Escherichia coli.
Authors: Haoyue Song; Yongfu Yang; Han Li; Jun Du; Zhousheng Hu; Yun-feng Chen; Ning Yang; Meng Mei; Zhiqiang Xiong; Ke Tang; Li Yi; Yu Zhang; Shihui Yang
PaperHistory-Driven Genetic Modification Design Technique Using a Domain-Specific Lexical Model for the Acceleration of DBTL Cycles for Microbial Cell Factories.
Nakazawa, Shiori; Imaichi, Osamu; Kogure, Takahisa et al. · 2021 · ACS synthetic biology · DOI
Full title: History-Driven Genetic Modification Design Technique Using a Domain-Specific Lexical Model for the Acceleration of DBTL Cycles for Microbial Cell Factories.
PaperHeterologous Production of Squalene from Glucose in Engineered Corynebacterium glutamicum Using Multiplex CRISPR Interference and High-Throughput Fermentation.
Jaehyun Park; B. Yu; Jong-il Choi et al. · 2018 · Journal of Agricultural and Food Chemistry · DOI
Full title: Heterologous Production of Squalene from Glucose in Engineered Corynebacterium glutamicum Using Multiplex CRISPR Interference and High-Throughput Fermentation.
Authors: Jaehyun Park; B. Yu; Jong-il Choi; H. Woo
PaperQuantitative Bioreactor Monitoring of Intracellular Bacterial Metabolites in <i>Clostridium</i> <i>autoethanogenum</i> Using Liquid Chromatography–Isotope Dilution Mass Spectrometry
Safo, Laudina; Abdelrazig, Salah; Grosse-Honebrink, Alexander et al. · 2021 · ACS Omega · DOI
Full title: Quantitative Bioreactor Monitoring of Intracellular Bacterial Metabolites in <i>Clostridium</i> <i>autoethanogenum</i> Using Liquid Chromatography–Isotope Dilution Mass Spectrometry
Authors: Safo, Laudina; Abdelrazig, Salah; Grosse-Honebrink, Alexander; Millat, Thomas; Henstra, Anne M.; Norman, Rupert; Thomas, Neil R.; Winzer, Klaus; Minton, Nigel P.; Kim, Dong‐Hyun; Barrett, David A.
PaperNon-native Pathway Engineering with CRISPRi for Carbon Dioxide Assimilation and Valued 5-Aminolevulinic Acid Synthesis in Escherichia coli Nissle
S. S. W. Effendi; I-Son Ng · 2024 · ACS Synthetic Biology · DOI
Full title: Non-native Pathway Engineering with CRISPRi for Carbon Dioxide Assimilation and Valued 5-Aminolevulinic Acid Synthesis in Escherichia coli Nissle
PaperMetabolic Profile of the Genome-Reduced Bacillus subtilis Strain IIG-Bs-27-39: An Attractive Chassis for Recombinant Protein Production
Rocío Aguilar Suárez; Michael Kohlstedt; Ayşegül Öktem et al. · 2024 · ACS Synthetic Biology · DOI
Full title: Metabolic Profile of the Genome-Reduced Bacillus subtilis Strain IIG-Bs-27-39: An Attractive Chassis for Recombinant Protein Production
Authors: Rocío Aguilar Suárez; Michael Kohlstedt; Ayşegül Öktem; Jolanda Neef; Yuzheng Wu; Kaiya Ikeda; Ken-Ichi Yoshida; Josef Altenbuchner; Christoph Wittmann; J. V. van Dijl
PaperEngineering Pheromone-Mediated Quorum Sensing with Enhanced Response Output Increases Fucosyllactose Production in Saccharomyces cerevisiae.
Mingyuan Xu; Mengtong Sun; Xiangfeng Meng et al. · 2022 · ACS Synthetic Biology · DOI
Full title: Engineering Pheromone-Mediated Quorum Sensing with Enhanced Response Output Increases Fucosyllactose Production in Saccharomyces cerevisiae.
PaperEstablishing a High-Yield Bacillus subtilis-Based Cell-Free Protein Synthesis System for In Vitro Prototyping and Natural Product Biosynthesis.
Xiangyang Ji; Wanqiu Liu; Z. Cao et al. · 2025 · ACS Synthetic Biology · DOI
Full title: Establishing a High-Yield Bacillus subtilis-Based Cell-Free Protein Synthesis System for In Vitro Prototyping and Natural Product Biosynthesis.
Authors: Xiangyang Ji; Wanqiu Liu; Z. Cao; Shuhui Huang; Jian Li
PaperRapid determination of underivatized pyroglutamic acid, glutamic acid, glutamine and other relevant amino acids in fermentation media by LC-MS-MS
Qu, Jun; Chen, Wei; Luo, Guoan et al. · 2001 · The Analyst · DOI
Full title: Rapid determination of underivatized pyroglutamic acid, glutamic acid, glutamine and other relevant amino acids in fermentation media by LC-MS-MS
PaperEnhancement of essential cofactors for in vivo biocatalysis.
Pattarawan Intasian; Chalermroj Sutthaphirom; Oliver Bodeit et al. · 2024 · Faraday discussions · DOI
Full title: Enhancement of essential cofactors for in vivo biocatalysis.
Authors: Pattarawan Intasian; Chalermroj Sutthaphirom; Oliver Bodeit; Duangthip Trisrivirat; Ninlapan Kimprasoot; Juthamas Jaroensuk; B. Bakker; E. Klipp; P. Chaiyen
PaperAn artificial gene for human porphobilinogen synthase allows comparison of an allelic variation implicated in susceptibility to lead poisoning.
Jaffe, E K; Volin, M; Bronson-Mullins, C R et al. · 2000 · The Journal of biological chemistry · DOI
Full title: An artificial gene for human porphobilinogen synthase allows comparison of an allelic variation implicated in susceptibility to lead poisoning.
Authors: Jaffe, E K; Volin, M; Bronson-Mullins, C R; Dunbrack, R L; Kervinen, J; Martins, J; Quinlan, J F; Sazinsky, M H; Steinhouse, E M; Yeung, A T
PaperDevelopment of a regulatable low-temperature protein expression system using the psychrotrophic bacterium, Shewanella livingstonensis Ac10, as the host.
Kawai, Soichiro; Kawamoto, Jun; Ogawa, Takuya et al. · 2019 · Bioscience, biotechnology, and biochemistry · DOI
Full title: Development of a regulatable low-temperature protein expression system using the psychrotrophic bacterium, Shewanella livingstonensis Ac10, as the host.
PaperA comprehensive study of glucose and oxygen gradients in a scaled-down model of recombinant HuGM-CSF production in thermoinduced Escherichia coli fed-batch cultures
Greta I. Reynoso-Cereceda; N. A. Valdez-Cruz; N. O. Pérez et al. · 2024 · Preparative Biochemistry & Biotechnology · DOI
Full title: A comprehensive study of glucose and oxygen gradients in a scaled-down model of recombinant HuGM-CSF production in thermoinduced Escherichia coli fed-batch cultures
Authors: Greta I. Reynoso-Cereceda; N. A. Valdez-Cruz; N. O. Pérez; M. Trujillo-Roldán
PaperGenome-Scale Metabolic Model of a Microbial Cell Factory (Brevibacillus thermoruber 423) with Multi-Industry Potentials for Exopolysaccharide Production
Songul Yasar Yildiz; E. Nikerel; Ebru Toksoy Öner · 2019 · Omics · DOI
Full title: Genome-Scale Metabolic Model of a Microbial Cell Factory (Brevibacillus thermoruber 423) with Multi-Industry Potentials for Exopolysaccharide Production
Authors: Songul Yasar Yildiz; E. Nikerel; Ebru Toksoy Öner
PaperHPLC Methods for Determination of Dihydroxyacetone and Glycerol in Fermentation Broth and Comparison with a Visible Spectrophotometric Method to Determine Dihydroxyacetone
Chen, Jie; Chen, Jie; Zhou, Chen · 2008 · Journal of Chromatographic Science · DOI
Full title: HPLC Methods for Determination of Dihydroxyacetone and Glycerol in Fermentation Broth and Comparison with a Visible Spectrophotometric Method to Determine Dihydroxyacetone
PaperProcess intensification at the expression system level for the production of 1-phosphate aldolase in antibiotic-free E. coli fed-batch cultures.
Pasini, Martina; Fernández-Castané, Alfred; Caminal, Gloria et al. · 2022 · Journal of industrial microbiology & biotechnology · DOI
Full title: Process intensification at the expression system level for the production of 1-phosphate aldolase in antibiotic-free E. coli fed-batch cultures.
PaperPerturbation of the peptidoglycan network and utilization of the signal recognition particle-dependent pathway enhances the extracellular production of a truncational mutant of CelA in Escherichia coli
Tae-Gu Kang; Seok-Hyun Hong; Gi-Beom Jeon et al. · 2021 · Journal of Industrial Microbiology & Biotechnology · DOI
Full title: Perturbation of the peptidoglycan network and utilization of the signal recognition particle-dependent pathway enhances the extracellular production of a truncational mutant of CelA in Escherichia coli
PaperEngineered multiple translation initiation sites: a novel tool to enhance protein production in Bacillus licheniformis and other industrially relevant bacteria
Manyu Zhang; Jing Song; Jun Xiao et al. · 2022 · Nucleic Acids Research · DOI
Full title: Engineered multiple translation initiation sites: a novel tool to enhance protein production in Bacillus licheniformis and other industrially relevant bacteria
Authors: Manyu Zhang; Jing Song; Jun Xiao; Jingjie Jin; C. Nomura; Shouwen Chen; Qin Wang
PaperUpdating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks
Paul Schaepe; M. Kwon; Birgit Baumann et al. · 2018 · bioRxiv · DOI
Full title: Updating genome annotation for the microbial cell factory Aspergillus niger using gene co-expression networks
Authors: Paul Schaepe; M. Kwon; Birgit Baumann; B. Gutschmann; Sascha Jung; Swantje Lenz; Benjamin M Nitsche; Norman Paege; T. Schuetze; T. Cairns; V. Meyer
PaperHigh-throughput developability assays enable library-scale identification of producible protein scaffold variants
Alexander W Golinski; Katelynn M Mischler; Sidharth Laxminarayan et al. · 2020 · Proceedings of the National Academy of Sciences of the United States of America · DOI
Full title: High-throughput developability assays enable library-scale identification of producible protein scaffold variants
Authors: Alexander W Golinski; Katelynn M Mischler; Sidharth Laxminarayan; Nicole Neurock; Matthew Fossing; Hannah Pichman; Stefano Martiniani; B. Hackel
Year: 2020
Venue: Proceedings of the National Academy of Sciences of the United States of America
PaperFood-Grade Microbial-Origin Antimicrobial Peptides: Structure-Activity Relationships, Mechanisms of Action, and Applications in Food Preservation.
Liang, Jiajian; Shi, Ruiyuan; Cheng, Lingyan et al. · 2026 · Comprehensive reviews in food science and food safety · DOI
Full title: Food-Grade Microbial-Origin Antimicrobial Peptides: Structure-Activity Relationships, Mechanisms of Action, and Applications in Food Preservation.
PaperIntegrated genomics and phenotype microarray analysis of Saccharomyces cerevisiae industrial strains for rice wine fermentation and recombinant protein production.
Son, Ye Ji; Jeon, Min-Seung; Moon, Hye Yun et al. · 2023 · Microbial biotechnology · DOI
Full title: Integrated genomics and phenotype microarray analysis of Saccharomyces cerevisiae industrial strains for rice wine fermentation and recombinant protein production.
Authors: Son, Ye Ji; Jeon, Min-Seung; Moon, Hye Yun; Kang, Jiwon; Jeong, Da Min; Lee, Dong Wook; Kim, Jae Ho; Lim, Jae Yun; Seo, Jeong-Ah; Jin, Jae-Hyung; Bahn, Yong-Sun; Eyun, Seong-Il; Kang, Hyun Ah
PaperThe XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering.
Full title: The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering.
PaperA Novel Chitin‐Based Purification System Using GAL1 Fusion Tags: Enhancing Recombinant Protein Production While Retaining Biological Activity
Yao-Kuang Tseng; Yun-Heng Lu; Yun Liu et al. · 2025 · Microbial Biotechnology · DOI
Full title: A Novel Chitin‐Based Purification System Using GAL1 Fusion Tags: Enhancing Recombinant Protein Production While Retaining Biological Activity
PaperRationale-based selection of optimal operating strategies and gene dosage impact on recombinant protein production in Komagataella phaffii (Pichia pastoris).
Nieto-Taype, Miguel Angel; Garrigós-Martínez, Javier; Sánchez-Farrando, Marc et al. · 2020 · Microbial biotechnology · DOI
Full title: Rationale-based selection of optimal operating strategies and gene dosage impact on recombinant protein production in Komagataella phaffii (Pichia pastoris).
Authors: Nieto-Taype, Miguel Angel; Garrigós-Martínez, Javier; Sánchez-Farrando, Marc; Valero, Francisco; Garcia-Ortega, Xavier; Montesinos-Seguí, José Luis
PaperDeveloping a metabolic model‐based fed‐batch feeding strategy for Pichia pastoris fermentation through fine‐tuning of the methanol utilization pathway
Mohammad Amin Boojari; Fatemeh Rajabi Ghaledari; E. Motamedian et al. · 2023 · Microbial Biotechnology · DOI
Full title: Developing a metabolic model‐based fed‐batch feeding strategy for Pichia pastoris fermentation through fine‐tuning of the methanol utilization pathway
Authors: Mohammad Amin Boojari; Fatemeh Rajabi Ghaledari; E. Motamedian; M. Soleimani; S. Shojaosadati
PaperThe Design of Fermentation and Biological Assay Procedures for Assessment of Penicillin Production in Populations of <i>Aspergillus nidulans</i>
Merrick, M. J.; Caten, C. E. · 1975 · Journal of Applied Bacteriology · DOI
Full title: The Design of Fermentation and Biological Assay Procedures for Assessment of Penicillin Production in Populations of <i>Aspergillus nidulans</i>
PaperAlternative binding proteins: anticalins - harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities.
Full title: Alternative binding proteins: anticalins - harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities.
PaperCRISPR/Cas9‐mediated gene knockout for DNA methyltransferase Dnmt3a in CHO cells displays enhanced transgenic expression and long‐term stability
Yan-Long Jia; Xiao Guo; Jiang-Tao Lu et al. · 2018 · Journal of Cellular and Molecular Medicine · DOI
Full title: CRISPR/Cas9‐mediated gene knockout for DNA methyltransferase Dnmt3a in CHO cells displays enhanced transgenic expression and long‐term stability
Authors: Yan-Long Jia; Xiao Guo; Jiang-Tao Lu; Xiao-Yin Wang; L. Qiu; Tian-yun Wang
PaperGenome‐based discovery of pachysiphine synthases in Tabernaemontana elegans
Enzo Lezin; Mickael Durand; Caroline Birer Williams et al. · 2024 · bioRxiv · DOI
Full title: Genome‐based discovery of pachysiphine synthases in Tabernaemontana elegans
Authors: Enzo Lezin; Mickael Durand; Caroline Birer Williams; Ana Luisa Lopez Vazquez; Thomas Perrot; Nicolas Gautron; Julien Pétrignet; Clément Cuello; Hans J. Jansen; Florent Magot; Sarah Szwarc; P. Le Pogam; M. Beniddir; K. Koudounas; A. Oudin; Benoit St-Pierre; N. Giglioli-Guivarc’h; Chao Sun; Nicolas Papon; Michael K. Jensen; Ron P. Dirks; S. O'connor; Sébastien Besseau; V. Courdavault
PaperComparison of VHH-Fc antibody production in Arabidopsis thaliana, Nicotiana benthamiana and Pichia pastoris.
De Meyer, Thomas; Laukens, Bram; Nolf, Jonah et al. · 2015 · Plant biotechnology journal · DOI
Full title: Comparison of VHH-Fc antibody production in Arabidopsis thaliana, Nicotiana benthamiana and Pichia pastoris.
Authors: De Meyer, Thomas; Laukens, Bram; Nolf, Jonah; Van Lerberge, Els; De Rycke, Riet; De Beuckelaer, Ans; De Buck, Sylvie; Callewaert, Nico; Depicker, Ann
PaperThe Yeast eIF2 Kinase Gcn2 Facilitates H2O2-Mediated Feedback Inhibition of Both Protein Synthesis and Endoplasmic Reticulum Oxidative Folding during Recombinant Protein Production.
Gast, Veronica; Campbell, Kate; Picazo, Cecilia et al. · 2021 · Applied and environmental microbiology · DOI
Full title: The Yeast eIF2 Kinase Gcn2 Facilitates H2O2-Mediated Feedback Inhibition of Both Protein Synthesis and Endoplasmic Reticulum Oxidative Folding during Recombinant Protein Production.
PaperPotassium and Sodium Salt Stress Characterization in the Yeasts Saccharomyces cerevisiae, Kluyveromyces marxianus, and Rhodotorula toruloides.
Illarionov, Aleksandr; Lahtvee, Petri-Jaan; Kumar, Rahul · 2021 · Applied and environmental microbiology · DOI
Full title: Potassium and Sodium Salt Stress Characterization in the Yeasts Saccharomyces cerevisiae, Kluyveromyces marxianus, and Rhodotorula toruloides.
PaperDraft Genome Sequence of Bacillus megaterium BHG1.1, a Strain Isolated from Bar-Headed Goose (Anser indicus) Feces on the Qinghai-Tibet Plateau.
Wang, Wen; Zheng, Si-Si; Sun, Hao et al. · 2016 · Genome announcements · DOI
Full title: Draft Genome Sequence of Bacillus megaterium BHG1.1, a Strain Isolated from Bar-Headed Goose (Anser indicus) Feces on the Qinghai-Tibet Plateau.
PaperModelling the Growth Limits (Growth/No Growth Interface) of <i>Escherichia coli</i> as a Function of Temperature, pH, Lactic Acid Concentration, and Water Activity
Presser, Kirsty Ann; Ross, T; Ratkowsky, David A. · 1998 · Applied and Environmental Microbiology · DOI
Full title: Modelling the Growth Limits (Growth/No Growth Interface) of <i>Escherichia coli</i> as a Function of Temperature, pH, Lactic Acid Concentration, and Water Activity
Authors: Presser, Kirsty Ann; Ross, T; Ratkowsky, David A.
PaperPortable CRISPR-Cas9 <sup>N</sup> System for Flexible Genome Engineering in Lactobacillus acidophilus, Lactobacillus gasseri, and Lactobacillus paracasei
Goh, Yong Jun; Barrangou, Rodolphe · 2021 · Applied and Environmental Microbiology · DOI
Full title: Portable CRISPR-Cas9 <sup>N</sup> System for Flexible Genome Engineering in Lactobacillus acidophilus, Lactobacillus gasseri, and Lactobacillus paracasei
PaperSafety evaluation of oubli fruit sweet protein (brazzein) derived from <i>K</i><i>omagataella phaffii,</i> intended for use as a sweetener in food and beverages
Lynch, Barry; Wang, Tina; Vo, Trung D. et al. · 2023 · Toxicology Research and Application · DOI
Full title: Safety evaluation of oubli fruit sweet protein (brazzein) derived from <i>K</i><i>omagataella phaffii,</i> intended for use as a sweetener in food and beverages
Authors: Lynch, Barry; Wang, Tina; Vo, Trung D.; Tafazoli, Shahrzad; Ryder, Jason A.
PaperSystematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems.
Della Cristina, Pietro; Castagna, Monica; Lombardi, Alessio et al. · 2015 · Microbial cell factories · DOI
Full title: Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems.
Authors: Della Cristina, Pietro; Castagna, Monica; Lombardi, Alessio; Barison, Erika; Tagliabue, Giovanni; Ceriotti, Aldo; Koutris, Ilias; Di Leandro, Luana; Giansanti, Francesco; Vago, Riccardo; Ippoliti, Rodolfo; Flavell, Sopsamorn U; Flavell, David J; Colombatti, Marco; Fabbrini, Maria Serena
PaperOptimization, characterization, comparison of self-assembly VLP of capsid protein L1 in yeast and reverse vaccinology design against human papillomavirus type 52.
Firdaus, Moh Egy Rahman; Mustopa, Apon Zaenal; Ekawati, Nurlaili et al. · 2023 · Journal, genetic engineering & biotechnology · DOI
Full title: Optimization, characterization, comparison of self-assembly VLP of capsid protein L1 in yeast and reverse vaccinology design against human papillomavirus type 52.
PaperConstruction of protease-deficient Candida boidinii strains useful for recombinant protein production: cloning and disruption of proteinase A gene (PEP4) and proteinase B gene (PRBI).
Komeda, Toshihiro; Sakai, Yasuyoshi; Kato, Nobuo et al. · 2002 · Bioscience, biotechnology, and biochemistry · DOI
Full title: Construction of protease-deficient Candida boidinii strains useful for recombinant protein production: cloning and disruption of proteinase A gene (PEP4) and proteinase B gene (PRBI).
PaperNoninvasive, On-Line Monitoring of the Biotransformation by Yeast of Glucose to Ethanol Using Dispersive Raman Spectroscopy and Chemometrics
Shaw, Adrian D.; Kaderbhai, Naheed; Jones, A. R. et al. · 1999 · Applied Spectroscopy · DOI
Full title: Noninvasive, On-Line Monitoring of the Biotransformation by Yeast of Glucose to Ethanol Using Dispersive Raman Spectroscopy and Chemometrics
Authors: Shaw, Adrian D.; Kaderbhai, Naheed; Jones, A. R.; Woodward, Andrew M.; Goodacre, Royston; Rowland, Jem J.; Kell, Douglas B.
PaperTowards animal-free dairy : investigating the impact of assembly conditions, composition adjustments, and casein modifications on casein micelles using reassembled sasein micelles
Full title: Towards animal-free dairy : investigating the impact of assembly conditions, composition adjustments, and casein modifications on casein micelles using reassembled sasein micelles
PaperSynthetic ratio computation for programming population composition and multicellular morphology
B. An; Tzu-Chieh Tang; Qian Zhang et al. · 2025 · bioRxiv · DOI
Full title: Synthetic ratio computation for programming population composition and multicellular morphology
Authors: B. An; Tzu-Chieh Tang; Qian Zhang; Teng Wang; Yanyi Wang; Kesheng Gan; Kun Liu; Dan Zhang; Yuzhu Liu; Yukui Pan; Min Yu; William M. Shaw; Qianyi Liang; Yaomin Wang; Chunbo Lou; Timothy K. Lu; George M. Church; Chao Zhong
PaperCXCR4(+)-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis.
O. Cano-Garrido; M. V. Céspedes; U. Unzueta et al. · 2016 · Nanomedicine · DOI
Full title: CXCR4(+)-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis.
Authors: O. Cano-Garrido; M. V. Céspedes; U. Unzueta; Paolo Saccardo; M. Roldán; A. Sánchez-Chardi; R. Cubarsi; E. Vázquez; R. Mangues; E. Garcia-Fruitós; A. Villaverde
PaperAn improved open-source software platform for high-throughput cultivation of phototrophic microorganisms and its application for salt tolerance experiments
Karolin Keil; Jonas Dierenbach; Ulrich M. Tillich et al. · 2019 · Journal of Cellular Biotechnology · DOI
Full title: An improved open-source software platform for high-throughput cultivation of phototrophic microorganisms and its application for salt tolerance experiments
Authors: Karolin Keil; Jonas Dierenbach; Ulrich M. Tillich; R. Steuer; M. Frohme
PaperFine-tuning of protein extraction from wall-deficient Chlamydomonas reinhardtii using liquid nitrogen and sonication-assisted cell disruption
A. Yıldırım · 2022 · Marine Science and Technology Bulletin · DOI
Full title: Fine-tuning of protein extraction from wall-deficient Chlamydomonas reinhardtii using liquid nitrogen and sonication-assisted cell disruption
PaperExpression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi.
Birchfield, Aaron S; McIntosh, Cecilia A · 2024 · Biotech (Basel (Switzerland)) · DOI
Full title: Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi.
PaperEngineering Pathways in Central Carbon Metabolism Help to Increase Glycan Production and Improve N-Type Glycosylation of Recombinant Proteins in E. coli.
Strutton, Benjamin; Jaffe, Stephen Rp; Evans, Caroline A et al. · 2019 · Bioengineering (Basel, Switzerland) · DOI
Full title: Engineering Pathways in Central Carbon Metabolism Help to Increase Glycan Production and Improve N-Type Glycosylation of Recombinant Proteins in E. coli.
Authors: Strutton, Benjamin; Jaffe, Stephen Rp; Evans, Caroline A; Fowler, Gregory Js; Dobson, Paul D; Pandhal, Jagroop; Wright, Phillip C
PaperComprehensive Review of Strategies for Lactic Acid Bacteria Production and Metabolite Enhancement in Probiotic Cultures: Multifunctional Applications in Functional Foods
Loo, J. van de; Oslan, Siti Nur Hazwani; Mokshin, Nur Anis Safiah et al. · 2025 · Fermentation · DOI
Full title: Comprehensive Review of Strategies for Lactic Acid Bacteria Production and Metabolite Enhancement in Probiotic Cultures: Multifunctional Applications in Functional Foods
Authors: Loo, J. van de; Oslan, Siti Nur Hazwani; Mokshin, Nur Anis Safiah; Othman, Rafidah; Amin, Zarina; Dejtisakdi, Wipawee; Prihanto, Asep Awaludin; Tan, Joo Shun
PaperPrecision Fermentation as a Frontier in Biofuel Production: Advances, Challenges, and Integration into Biorefineries
D. B. Pereira; Giovanna Lima-Silva; L. Soares et al. · 2026 · Fermentation · DOI
Full title: Precision Fermentation as a Frontier in Biofuel Production: Advances, Challenges, and Integration into Biorefineries
Authors: D. B. Pereira; Giovanna Lima-Silva; L. Soares; L. Aguiar; A. S. Vasconcelos; V. Pessoa; Roberta Pozzan; J. L. Serra; C. Sales-Campos; L. Chevreuil; W. J. Martínez-Burgos
PaperCheese Whey Valorization via Microbial Fermentation (Lactic Acid Bacteria, Yeasts/Fungi, and Microalgae), Postbiotic Production, and Whey-Based Encapsulation Strategies
Tlalli Uribe-Velázquez; Cesar E. Najar-Almanzor; Francisco R. Osuna-Orozco et al. · 2026 · Fermentation · DOI
Full title: Cheese Whey Valorization via Microbial Fermentation (Lactic Acid Bacteria, Yeasts/Fungi, and Microalgae), Postbiotic Production, and Whey-Based Encapsulation Strategies
Authors: Tlalli Uribe-Velázquez; Cesar E. Najar-Almanzor; Francisco R. Osuna-Orozco; Félix Arto-Paz; C. Valdés; L. Garcia-Amezquita; Danay Carrillo-Nieves; Tomás García-Cayuela
PaperAutomatic Control of Chemolithotrophic Cultivation of Cupriavidus necator: Optimization of Oxygen Supply for Enhanced Bioplastic Production
Lambauer, Vera; Permann, Alexander; Petrášek, Zdeněk et al. · 2023 · Fermentation · DOI
Full title: Automatic Control of Chemolithotrophic Cultivation of Cupriavidus necator: Optimization of Oxygen Supply for Enhanced Bioplastic Production
PaperPrecision Biomanufacturing with Lactic Acid Bacteria: From Ancestral Fermentations to Technological Innovation and Future Prospects for Next-Generation Functional Foods
Bustos, Ana Yanina; Gerez, Carla Luciana · 2026 · Fermentation · DOI
Full title: Precision Biomanufacturing with Lactic Acid Bacteria: From Ancestral Fermentations to Technological Innovation and Future Prospects for Next-Generation Functional Foods
PaperDevelopment of a Molasses-Based Medium for Agrobacterium tumefaciens Fermentation for Application in Plant-Based Recombinant Protein Production
Nisit Watthanasakphuban; L. Nguyen; Yu-Shen Cheng et al. · 2023 · Fermentation · DOI
Full title: Development of a Molasses-Based Medium for Agrobacterium tumefaciens Fermentation for Application in Plant-Based Recombinant Protein Production
Authors: Nisit Watthanasakphuban; L. Nguyen; Yu-Shen Cheng; P. Show; M. Sriariyanun; M. Koffas; K. Rattanaporn
PaperEnhancing Sustainability and Productivity in Komagataella phaffii Fermentation: A Techno-Economic Comparison of Fed-Batch and Continuous Cultivation with Mixed Induction Strategies
Almir Yamanie; Salomé de Sá Magalhães; A. R. Wijayadikusumah et al. · 2026 · Fermentation · DOI
Full title: Enhancing Sustainability and Productivity in Komagataella phaffii Fermentation: A Techno-Economic Comparison of Fed-Batch and Continuous Cultivation with Mixed Induction Strategies
Authors: Almir Yamanie; Salomé de Sá Magalhães; A. R. Wijayadikusumah; Neni Nurainy; Eli Keshavarz-Moore
PaperImprovement of L-asparaginase, an Anticancer Agent of Aspergillus arenarioides EAN603 in Submerged Fermentation Using a Radial Basis Function Neural Network with a Specific Genetic Algorithm (RBFNN-GA)
Alzaeemi, Shehab Abdulhabib; Noman, Efaq Ali; Al-shaibani, Muhanna Mohammed et al. · 2023 · Fermentation · DOI
Full title: Improvement of L-asparaginase, an Anticancer Agent of Aspergillus arenarioides EAN603 in Submerged Fermentation Using a Radial Basis Function Neural Network with a Specific Genetic Algorithm (RBFNN-GA)
PaperA Deep Learning Approach to Optimize Recombinant Protein Production in Escherichia coli Fermentations
Domenico Bonanni; Mattia Litrico; Waqar Ahmed et al. · 2023 · Fermentation · DOI
Full title: A Deep Learning Approach to Optimize Recombinant Protein Production in Escherichia coli Fermentations
Authors: Domenico Bonanni; Mattia Litrico; Waqar Ahmed; Pietro Morerio; Tiziano Cazzorla; E. Spaccapaniccia; F. Cattani; M. Allegretti; A. Beccari; Alessio Del Bue; F. Martin
PaperProduction of Active Recombinant Hyaluronidase Inclusion Bodies from Apis mellifera in E. coli Bl21(DE3) and characterization by FT-IR Spectroscopy.
Schwaighofer, Andreas; Ablasser, Sarah; Lux, Laurin et al. · 2020 · International journal of molecular sciences · DOI
Full title: Production of Active Recombinant Hyaluronidase Inclusion Bodies from Apis mellifera in E. coli Bl21(DE3) and characterization by FT-IR Spectroscopy.
PaperUntargeted Lipidomics Analysis of the Cyanobacterium Synechocystis sp. PCC 6803: Lipid Composition Variation in Response to Alternative Cultivation Setups and to Gene Deletion
Weronika Hewelt-Belka; Á. Kot-Wasik; P. Tamagnini et al. · 2020 · International Journal of Molecular Sciences · DOI
Full title: Untargeted Lipidomics Analysis of the Cyanobacterium Synechocystis sp. PCC 6803: Lipid Composition Variation in Response to Alternative Cultivation Setups and to Gene Deletion
Authors: Weronika Hewelt-Belka; Á. Kot-Wasik; P. Tamagnini; Paulo Oliveira
Year: 2020
Venue: International Journal of Molecular Sciences
PaperA New Expression System Based on Psychrotolerant Debaryomyces macquariensis Yeast and Its Application to the Production of Cold-Active β-d-Galactosidase from Paracoccus sp. 32d.
Wanarska, Marta; Krajewska-Przybyszewska, Ewelina; Wicka-Grochocka, Monika et al. · 2022 · International journal of molecular sciences · DOI
Full title: A New Expression System Based on Psychrotolerant Debaryomyces macquariensis Yeast and Its Application to the Production of Cold-Active β-d-Galactosidase from Paracoccus sp. 32d.
PaperReconstruction of a Genome-Scale Metabolic Model for Aspergillus oryzae Engineered Strain: A Potent Computational Tool for Enhancing Cordycepin Production.
Raethong, Nachon; Jeennor, Sukanya; Anantayanon, Jutamas et al. · 2025 · International journal of molecular sciences · DOI
Full title: Reconstruction of a Genome-Scale Metabolic Model for Aspergillus oryzae Engineered Strain: A Potent Computational Tool for Enhancing Cordycepin Production.
PaperThe Sugar Metabolic Model of Aspergillus niger Can Only Be Reliably Transferred to Fungi of Its Phylum
Jiajia Li; Tania Chroumpi; S. Garrigues et al. · 2022 · Journal of Fungi · DOI
Full title: The Sugar Metabolic Model of Aspergillus niger Can Only Be Reliably Transferred to Fungi of Its Phylum
Authors: Jiajia Li; Tania Chroumpi; S. Garrigues; R. S. Kun; Jiali Meng; Sonia Salazar-Cerezo; M. V. Aguilar-Pontes; Yu Zhang; S. Tejomurthula; A. Lipzen; Vivian Ng; Chaevien S. Clendinen; N. Tolić; I. Grigoriev; A. Tsang; M. Mäkelä; B. Snel; Mao Peng; R. D. de Vries
PaperProduction of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unveiling Antimicrobial and Anticancer Functionalities.
Yen, Chih-Ching; Wu, Pei-Ying; Ou-Yang, Huan et al. · 2024 · International journal of molecular sciences · DOI
Full title: Production of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unveiling Antimicrobial and Anticancer Functionalities.
PaperQuantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris.
Jordà, Joel; Rojas, Hugo Cueto; Carnicer, Marc et al. · 2014 · Metabolites · DOI
Full title: Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris.
Authors: Jordà, Joel; Rojas, Hugo Cueto; Carnicer, Marc; Wahl, Aljoscha; Ferrer, Pau; Albiol, Joan
PaperRecombinant Inga Laurina Trypsin Inhibitor (ILTI) Production in Komagataella Phaffii Confirms Its Potential Anti-Biofilm Effect and Reveals an Anti-Tumoral Activity
F. C. Carneiro; S. Weber; O. N. Silva et al. · 2018 · Microorganisms · DOI
Full title: Recombinant Inga Laurina Trypsin Inhibitor (ILTI) Production in Komagataella Phaffii Confirms Its Potential Anti-Biofilm Effect and Reveals an Anti-Tumoral Activity
Authors: F. C. Carneiro; S. Weber; O. N. Silva; A. Jacobowski; Marcelo H. S. Ramada; M. L. Macedo; O. Franco; N. Parachin
PaperDeionococcus proteotlycius Genomic Library Exploration Enhances Oxidative Stress Resistance and Poly-3-hydroxybutyrate Production in Recombinant Escherichia coli
Seul-Ki Yang; Soyoung Jeong; Inwoo Baek et al. · 2023 · Microorganisms · DOI
Full title: Deionococcus proteotlycius Genomic Library Exploration Enhances Oxidative Stress Resistance and Poly-3-hydroxybutyrate Production in Recombinant Escherichia coli
PaperProduction of Raw Starch-Digesting Amylolytic Preparation in Yarrowia lipolytica and Its Application in Biotechnological Synthesis of Lactic Acid and Ethanol
Aleksandra Gęsicka; M. Borkowska; W. Białas et al. · 2020 · Microorganisms · DOI
Full title: Production of Raw Starch-Digesting Amylolytic Preparation in Yarrowia lipolytica and Its Application in Biotechnological Synthesis of Lactic Acid and Ethanol
Authors: Aleksandra Gęsicka; M. Borkowska; W. Białas; Paulina Kaczmarek; E. Celińska
PaperA Non-linear Model Predictive Control Based on Grey-Wolf Optimization Using Least-Square Support Vector Machine for Product Concentration Control in l-Lysine Fermentation
Wang, Bo; Shahzad, Muhammad; Zhu, Xianglin et al. · 2020 · Sensors · DOI
Full title: A Non-linear Model Predictive Control Based on Grey-Wolf Optimization Using Least-Square Support Vector Machine for Product Concentration Control in l-Lysine Fermentation
PaperContinuous Manufacturing of Recombinant Drugs: Comprehensive Analysis of Cost Reduction Strategies, Regulatory Pathways, and Global Implementation
Full title: Continuous Manufacturing of Recombinant Drugs: Comprehensive Analysis of Cost Reduction Strategies, Regulatory Pathways, and Global Implementation
PaperDielectric Spectroscopy and Optical Density Measurement for the Online Monitoring and Control of Recombinant Protein Production in Stably Transformed Drosophila melanogaster S2 Cells.
Zitzmann, Jan; Weidner, Tobias; Eichner, Gerrit et al. · 2018 · Sensors (Basel, Switzerland) · DOI
Full title: Dielectric Spectroscopy and Optical Density Measurement for the Online Monitoring and Control of Recombinant Protein Production in Stably Transformed Drosophila melanogaster S2 Cells.
PaperCRISPR/Cas9-Driven Engineering of AcMNPV Using Dual gRNA for Optimized Recombinant Protein Production
Rocco Valente; Joaquín Poodts; J. M. Birenbaum et al. · 2025 · Viruses · DOI
Full title: CRISPR/Cas9-Driven Engineering of AcMNPV Using Dual gRNA for Optimized Recombinant Protein Production
Authors: Rocco Valente; Joaquín Poodts; J. M. Birenbaum; M. S. Rodriguez; I. Smith; J. A. Simonin; Franco Uriel Cuccovia Warlet; A. Trabucchi; S. Herrero; M. V. Miranda; M. Belaich; A. Targovnik
PaperProduction and Optimization of LTE, a Leishmania tarentolae Derived Cell-Free Protein Expression System for Recombinant Protein Production.
Johnston, Wayne A; Palma, Juan Alfaro; Alexandrov, Kirill · 2024 · Journal of visualized experiments : JoVE · DOI
Full title: Production and Optimization of LTE, a Leishmania tarentolae Derived Cell-Free Protein Expression System for Recombinant Protein Production.
Authors: Johnston, Wayne A; Palma, Juan Alfaro; Alexandrov, Kirill
PaperGeneration of Active Bovine Terminal Deoxynucleotidyl Transferase (TdT) in E.coli
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Full title: Generation of Active Bovine Terminal Deoxynucleotidyl Transferase (TdT) in E.coli
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PaperRecombinant Protein Purification using Composite Polyacrylamide-Nanocrystalline Cryogel Monolith Column and Carbohydrate-Binding Module Family 64 as Affinity Tag.
Danaeifar, Mohsen; Veisi Malekshahi, Ziba; Kazemi-Lomedasht, Fatemeh et al. · 2022 · Reports of biochemistry & molecular biology · DOI
Full title: Recombinant Protein Purification using Composite Polyacrylamide-Nanocrystalline Cryogel Monolith Column and Carbohydrate-Binding Module Family 64 as Affinity Tag.
Authors: Danaeifar, Mohsen; Veisi Malekshahi, Ziba; Kazemi-Lomedasht, Fatemeh; Mazlomi, Mohammad Ali
Year: 2022
Venue: Reports of biochemistry & molecular biology
PaperRecombinant Expression of Taq DNA Polymerase Using pBluescript SK(+) and Chromatography-free Purification: A Cost-effective Strategy for Low-resource Laboratories
Nolan Ramanjulu; Bibi Rafeiza Khan · 2025 · Journal of Advances in Biology & Biotechnology · DOI
Full title: Recombinant Expression of Taq DNA Polymerase Using pBluescript SK(+) and Chromatography-free Purification: A Cost-effective Strategy for Low-resource Laboratories
Authors: Nolan Ramanjulu; Bibi Rafeiza Khan
Year: 2025
Venue: Journal of Advances in Biology & Biotechnology
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PaperMetabolic engineering of Escherichia coli: construction and characterization of a gltA (citrate synthase) knockout mutant.
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Full title: Metabolic engineering of Escherichia coli: construction and characterization of a gltA (citrate synthase) knockout mutant.
Authors: Vandedrinck, S; Deschamps, G; Sablon, E; Vandamme, E J
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Venue: Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)
PaperMultin stage Identification Model,Algorithm And Appication of Nonlinear Dynaic System
Xiu, Zhilong · 2006 · Yunchou yu guanli
Full title: Multin stage Identification Model,Algorithm And Appication of Nonlinear Dynaic System
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PaperMolecular Cloning, Expression and Characterization of Plasmid Encoding Rhomboid 4 (ROM4) of Tachyzoite of Toxoplasma gondii RH Strain
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Full title: Molecular Cloning, Expression and Characterization of Plasmid Encoding Rhomboid 4 (ROM4) of Tachyzoite of Toxoplasma gondii RH Strain
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Year: 2017
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PaperPeptidases affecting recombinant protein production by Streptomyces lividans.
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Full title: Peptidases affecting recombinant protein production by Streptomyces lividans.
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Ciarkowska, Anna; Jakubowska, Anna · 2013 · Postepy biochemii
Full title: [Pichia pastoris as an expression system for recombinant protein production].
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Paper[Recombinant protein production in Escherichia coli].
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PaperSoft-sensing Modeling Based on MLS-SVM Inversion for L-lysine Fermentation Processes
Wang, Bo; Ji, Xiaofu · 2015 · SHILAP Revista de lepidopterología
Full title: Soft-sensing Modeling Based on MLS-SVM Inversion for L-lysine Fermentation Processes
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Year: 2015
Venue: SHILAP Revista de lepidopterología
PaperSoft sensing modeling based on dynamic fuzzy neural network for penicillin fermentation
Huang, Yonghong; Cheng, Hao; Huang, Li et al. · 2012 · Chinese Control Conference
Full title: Soft sensing modeling based on dynamic fuzzy neural network for penicillin fermentation
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PaperSoft measurement of lysine fermentation based on FCM and integrated Gaussian process regression
Xiaof, JI · 2015 · Caai Transactions on Intelligent Systems
Full title: Soft measurement of lysine fermentation based on FCM and integrated Gaussian process regression
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Year: 2015
Venue: Caai Transactions on Intelligent Systems
PaperSoft Sensor of Lysien Fermentation Biological Parameters Based on Relevance Vector Machine
Li, Huang · 2013 · Journal of Huaqiao University
Full title: Soft Sensor of Lysien Fermentation Biological Parameters Based on Relevance Vector Machine
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Year: 2013
Venue: Journal of Huaqiao University
PaperSoft Sensor Modeling Based on Biological Variables of Marine Protease Fermentation Process
Yong-hon, Huang · 2013 · Information and Computation
Full title: Soft Sensor Modeling Based on Biological Variables of Marine Protease Fermentation Process
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Year: 2013
Venue: Information and Computation
PaperSimultaneous Determination of Ethanol,Sugar and Organic Acids in Kluveromyces marxious Broth by High Performance Liquid Chromatography-Ultraviolet/Refractive Index Detector
Wen, Yuan · 2009
Full title: Simultaneous Determination of Ethanol,Sugar and Organic Acids in Kluveromyces marxious Broth by High Performance Liquid Chromatography-Ultraviolet/Refractive Index Detector
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Year: 2009
PaperSoft Sensing Modeling Based on Generalized Dynamic Fuzzy Neural Network for Microbial Fermentation Process
Full title: Soft Sensing Modeling Based on Generalized Dynamic Fuzzy Neural Network for Microbial Fermentation Process
Authors: Yong-hon, Huang
Year: 2013
Venue: Yibiao jishu yu chuanganqi
PaperStudy on efficient determination method of poly-γ-glutamic acid in fermentation broth
Li, Hui‐Jing · 2012 · Science and Technology of Food Industry
Full title: Study on efficient determination method of poly-γ-glutamic acid in fermentation broth
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Year: 2012
Venue: Science and Technology of Food Industry
PaperSoft Sensor Model of Fermentation Process Based on NN-MIV Variable Selection
Shuan, YU · 2015 · Control Engineering of China
Full title: Soft Sensor Model of Fermentation Process Based on NN-MIV Variable Selection
Authors: Shuan, YU
Year: 2015
Venue: Control Engineering of China
PaperSoft-sensor modeling method in a fermentation process based on the samples of a sparse Gaussian process
Ku, He · 2014 · Journal of Beijing University of Chemical Technology
Full title: Soft-sensor modeling method in a fermentation process based on the samples of a sparse Gaussian process
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Venue: Journal of Beijing University of Chemical Technology
PaperThe use of gene fusions to protein A and protein G in immunology and biotechnology.
Ståhl, S; Nygren, P A · 1997 · Pathologie-biologie
Full title: The use of gene fusions to protein A and protein G in immunology and biotechnology.
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Venue: Pathologie-biologie
PaperThe Use of Transcription Terminators to Generate Transgenic Lines of Chinese Hamster Ovary Cells (CHO) with Stable and High Level of Reporter Gene Expression.
Gasanov, N B; Toshchakov, S V; Georgiev, P G et al. · 2015 · Acta naturae
Full title: The Use of Transcription Terminators to Generate Transgenic Lines of Chinese Hamster Ovary Cells (CHO) with Stable and High Level of Reporter Gene Expression.
Authors: Gasanov, N B; Toshchakov, S V; Georgiev, P G; Maksimenko, O G
Year: 2015
Venue: Acta naturae
PaperStudies on mycelia content measuring of Penicillium chrysogenum in fermentation
Wang, Shiying · 2007 · Hebei Nongye Daxue xuebao
Full title: Studies on mycelia content measuring of Penicillium chrysogenum in fermentation
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Year: 2007
Venue: Hebei Nongye Daxue xuebao
ThesisMetabolic modeling and engineering of Escherichia coli to reduce acetate formation in recombinant DNA-fermentations
De Mey, Marjan · 2007 · Ghent University (Universiteit Gent) · DOI
Full title: Metabolic modeling and engineering of Escherichia coli to reduce acetate formation in recombinant DNA-fermentations
ThesisExpression and production of the Saccharomyces cerevisiae haze protective factor 2 for sensory studies and further investigation into the role of glycosylation.
Macintyre, Oenone Jean · 2008 · University of Adelaide · DOI
Full title: Expression and production of the Saccharomyces cerevisiae haze protective factor 2 for sensory studies and further investigation into the role of glycosylation.
ThesisSpoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially therapeutic recombinant glycoprotein factor H
Devlin, John Patrick · 2018 · The University of Edinburgh · DOI
Full title: Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially therapeutic recombinant glycoprotein factor H
ThesisEngineering Escherichia coli to improve its N-linked glycosylation capabilities and the development of a new method of quantifying production
Strutton, Benjamin · 2016 · White Rose eTheses (Leeds, Sheffield, York) · DOI
Full title: Engineering Escherichia coli to improve its N-linked glycosylation capabilities and the development of a new method of quantifying production
Authors: Strutton, Benjamin
Year: 2016
Venue: White Rose eTheses (Leeds, Sheffield, York)
ThesisEngineering precursor supply for the high-level production of ergothioneine in Saccharomyces cerevisiae.
van der Hoek, Steven A; Rusnák, Matej; Wang, Guokun et al. · 2022 · University of Liverpool · DOI
Full title: Engineering precursor supply for the high-level production of ergothioneine in Saccharomyces cerevisiae.
Authors: van der Hoek, Steven A; Rusnák, Matej; Wang, Guokun; Stanchev, Lyubomir Dimitrov; de Fátima Alves, Luana; Jessop-Fabre, Mathew M; Paramasivan, Kalaivani; Jacobsen, Irene Hjorth; Sonnenschein, Nikolaus; Martínez, José L; Darbani, Behrooz; Kell, Douglas B; Borodina, Irina
ThesisIdentification and Engineering of Transporters for Efficient Melatonin Production in Escherichia coli
Yang, Lei; Malla, Sailesh; Oezdemir, Emre et al. · 2022 · University of Liverpool · DOI
Full title: Identification and Engineering of Transporters for Efficient Melatonin Production in Escherichia coli
Authors: Yang, Lei; Malla, Sailesh; Oezdemir, Emre; Kim, Se Hyeuk; Lennen, Rebecca; Christensen, Hanne B; Christensen, Ulla; Munro, Lachlan J; Herrgard, Markus J; Kell, Douglas B; Palsson, Bernhard O
ThesisSelective release of overexpressed recombinant proteins from E. coli cells facilitates one-step chromatographic purification of peptide-tagged green fluorescent protein variants
Kaveh-Baghbaderani Y, Blank-Shim SA, Koch T, Berensmeier S: · 2018 · Technical University of Munich (TUM) · DOI
Full title: Selective release of overexpressed recombinant proteins from E. coli cells facilitates one-step chromatographic purification of peptide-tagged green fluorescent protein variants
Authors: Kaveh-Baghbaderani Y, Blank-Shim SA, Koch T, Berensmeier S:
ThesisImpact of Elevated Levels of Dissolved CO 2 on Performance and Proteome Response of an Industrial 2'-Fucosyllactose Producing Escherichia coli Strain
Gecse, Greta; Vente, André; Kilstrup, Mogens et al. · 2022 · Technical University of Denmark (DTU) · DOI
Full title: Impact of Elevated Levels of Dissolved CO 2 on Performance and Proteome Response of an Industrial 2'-Fucosyllactose Producing Escherichia coli Strain
ThesisOptimising the biosynthesis of oxygenated and acetylated Taxol precursors in Saccharomyces cerevisiae using advanced bioprocessing strategies
Walls, Laura E.; Malci, Koray; Nowrouzi, Behnaz et al. · 2021 · Technical University of Denmark (DTU) · DOI
Full title: Optimising the biosynthesis of oxygenated and acetylated Taxol precursors in Saccharomyces cerevisiae using advanced bioprocessing strategies
Authors: Walls, Laura E.; Malci, Koray; Nowrouzi, Behnaz; Li, Rachel; d'Espaux, Leo; Wong, Jeff; Dennis, Jonathan A.; Semiao, Andrea J C; Wallace, Stephen; Martinez, José L.; id_orcid 0000-0002-4490-8534; Keasling, Jay D.; Rios-Solis, Leonardo
ThesisBurden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan et al. · 2022 · Technical University of Denmark (DTU) · DOI
Full title: Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Authors: Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan; Jensen, Michael Krogh; id_orcid 0000-0001-7574-4707; Workman, Christopher T.; id_orcid 0000-0002-2210-3743
ThesisMinimizing acetate formation from overflow metabolism in Escherichia coli : comparison of genetic engineering strategies to improve robustness toward sugar gradients in large-scale fermentation processes
Gecse, Greta; Labunskaite, Rugile; id_orcid 0009-0007-8147-9023 et al. · 2024 · Technical University of Denmark (DTU) · DOI
Full title: Minimizing acetate formation from overflow metabolism in Escherichia coli : comparison of genetic engineering strategies to improve robustness toward sugar gradients in large-scale fermentation processes
ThesisA Novel Bio-Purification Process Employing an Engineered E. coli Strain for Downstream Processing of Lactic Acid Solutions from the Fermentation of Agro-Industrial by-Products
Nastouli, Alexandra; Moschona, Alexandra; Bizirtsakis, Panagiotis A. et al. · 2024 · Technical University of Denmark (DTU) · DOI
Full title: A Novel Bio-Purification Process Employing an Engineered E. coli Strain for Downstream Processing of Lactic Acid Solutions from the Fermentation of Agro-Industrial by-Products
ThesisSynthetic expression system enhances recombinant protein production in Aspergillus oryzae
van der Luijt, Casper Robert Balten; Rekdal, Vayu Maini; Chen, Yan et al. · 2024 · Technical University of Denmark (DTU) · DOI
Full title: Synthetic expression system enhances recombinant protein production in Aspergillus oryzae
Authors: van der Luijt, Casper Robert Balten; Rekdal, Vayu Maini; Chen, Yan; J. Petzold, Christopher; Keasling, Jay; Jahn, Leonie; Sommer, Morten Otto Alexander; id_orcid 0000-0003-4005-5674
ThesisAutomated Electrochemical Glucose Biosensor Platform as an Efficient Tool Toward On-Line Fermentation Monitoring:Novel Application Approaches and Insights
Pontius, Katrin; Semenova, Daria; Silina, Yuliya E. et al. · 2020 · Technical University of Denmark (DTU) · DOI
Full title: Automated Electrochemical Glucose Biosensor Platform as an Efficient Tool Toward On-Line Fermentation Monitoring:Novel Application Approaches and Insights
ThesisEstablishing Debaryomyces hansenii as a superior cell factory for the green transition: optimizing the use of industrial side-streams and complex feedstock for biotech applications
Duran, Mònica Estrada; id_orcid 0000-0002-8377-249X · 2024 · Technical University of Denmark (DTU) · DOI
Full title: Establishing Debaryomyces hansenii as a superior cell factory for the green transition: optimizing the use of industrial side-streams and complex feedstock for biotech applications
ThesisOpen (non-sterile) cultivations of Debaryomyces hansenii for recombinant protein production combining industrial side-streams with high salt content
Estrada, Mònica; id_orcid 0000-0002-8377-249X; Navarrete, Clara et al. · 2023 · Technical University of Denmark (DTU) · DOI
Full title: Open (non-sterile) cultivations of Debaryomyces hansenii for recombinant protein production combining industrial side-streams with high salt content
ThesisIntegrated Optimization of Hemoprotein Biomanufacturing in Yeast:From Upstream Engineering of Heme Synthesis to Downstream Purification of Human Hemoglobin
Frost, August Thinggaard · 2023 · Technical University of Denmark (DTU) · DOI
Full title: Integrated Optimization of Hemoprotein Biomanufacturing in Yeast:From Upstream Engineering of Heme Synthesis to Downstream Purification of Human Hemoglobin
ThesisA strategy for successful dual-species protein expression of genes with non-optimal codon usage destined for bacterial and yeast cell factories
Wäneskog, Marcus; Rasmussen, Trine Bertram; Jensen, Emil D. et al. · 2024 · Technical University of Denmark (DTU) · DOI
Full title: A strategy for successful dual-species protein expression of genes with non-optimal codon usage destined for bacterial and yeast cell factories
ThesisSynthetic expression system enhances recombinant protein production in Aspergillus oryzae
van der Luijt, Casper Robert Balten; Rekdal, Vayu Maini; Chen, Yan et al. · 2024 · Technical University of Denmark (DTU) · DOI
Full title: Synthetic expression system enhances recombinant protein production in Aspergillus oryzae
Authors: van der Luijt, Casper Robert Balten; Rekdal, Vayu Maini; Chen, Yan; J. Petzold, Christopher; Keasling, Jay; Jahn, Leonie; Sommer, Morten Otto Alexander; id_orcid 0000-0003-4005-5674
ThesisBio-succinic acid production, up to pilot scale, by fermentation of industrial candy waste with Actinobacillus succinogenes 130Z and its downstream purification process
Lithourgidis, Antonios A.; Kotsopoulos, Thomas A.; Kalamaras, Sotirios D. et al. · 2023 · Technical University of Denmark (DTU) · DOI
Full title: Bio-succinic acid production, up to pilot scale, by fermentation of industrial candy waste with Actinobacillus succinogenes 130Z and its downstream purification process
ThesisDynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica
Kerssemakers, Abraham Antonius Johannes; id_orcid 0000-0002-3462-4642; Øzmerih, Süleyman et al. · 2023 · Technical University of Denmark (DTU) · DOI
Full title: Dynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica
Authors: Kerssemakers, Abraham Antonius Johannes; id_orcid 0000-0002-3462-4642; Øzmerih, Süleyman; Sin, Gürkan; id_orcid 0000-0003-0513-4502; Sudarsan, Suresh
ThesisExploration of the potential of nonconventional yeasts as cell factories by physiological characterisation and development of new expression tools
Hansen, Sebastian Ro Toft · 2020 · Technical University of Denmark (DTU) · DOI
Full title: Exploration of the potential of nonconventional yeasts as cell factories by physiological characterisation and development of new expression tools
ThesisProduction of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA: ethanol O-acyltransferase Eht1 or Eeb1
Zhuang, Shiwen; Fu, Junshu; Powell, Chris et al. · 2015 · Technical University of Denmark (DTU) · DOI
Full title: Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA: ethanol O-acyltransferase Eht1 or Eeb1
ThesisRecombinant β-Carotene Production by Yarrowia lipolytica - Assessing the Potential of Micro-Scale Fermentation Analysis in Cell Factory Design and Bioreaction Optimization
Jacobsen, Irene Hjorth; Ledesma-Amaro, Rodrigo; Martinez, José Luis et al. · 2020 · Technical University of Denmark (DTU) · DOI
Full title: Recombinant β-Carotene Production by Yarrowia lipolytica - Assessing the Potential of Micro-Scale Fermentation Analysis in Cell Factory Design and Bioreaction Optimization
ThesisImpact of Elevated Levels of Dissolved CO 2 on Performance and Proteome Response of an Industrial 2'-Fucosyllactose Producing Escherichia coli Strain
Gecse, Greta; Vente, André; Kilstrup, Mogens et al. · 2022 · Technical University of Denmark (DTU) · DOI
Full title: Impact of Elevated Levels of Dissolved CO 2 on Performance and Proteome Response of an Industrial 2'-Fucosyllactose Producing Escherichia coli Strain
ThesisConstruct design, production, and characterization of Plasmodium falciparum 48/45 R0.6C subunit protein produced in Lactococcus lactis as candidate vaccine
Singh, Susheel K; Roeffen, Will; Mistarz, Ulrik H et al. · 2017 · University of Copenhagen · DOI
Full title: Construct design, production, and characterization of Plasmodium falciparum 48/45 R0.6C subunit protein produced in Lactococcus lactis as candidate vaccine
ThesisOptimising the biosynthesis of oxygenated and acetylated Taxol precursors in Saccharomyces cerevisiae using advanced bioprocessing strategies
Walls, Laura E.; Malci, Koray; Nowrouzi, Behnaz et al. · 2021 · Technical University of Denmark (DTU) · DOI
Full title: Optimising the biosynthesis of oxygenated and acetylated Taxol precursors in Saccharomyces cerevisiae using advanced bioprocessing strategies
Authors: Walls, Laura E.; Malci, Koray; Nowrouzi, Behnaz; Li, Rachel; d'Espaux, Leo; Wong, Jeff; Dennis, Jonathan A.; Semiao, Andrea J C; Wallace, Stephen; Martinez, José L.; id_orcid 0000-0002-4490-8534; Keasling, Jay D.; Rios-Solis, Leonardo
ThesisBurden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan et al. · 2022 · Technical University of Denmark (DTU) · DOI
Full title: Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Authors: Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan; Jensen, Michael Krogh; id_orcid 0000-0001-7574-4707; Workman, Christopher T.; id_orcid 0000-0002-2210-3743
ThesisMinimizing acetate formation from overflow metabolism in Escherichia coli : comparison of genetic engineering strategies to improve robustness toward sugar gradients in large-scale fermentation processes
Gecse, Greta; Labunskaite, Rugile; id_orcid 0009-0007-8147-9023 et al. · 2024 · Technical University of Denmark (DTU) · DOI
Full title: Minimizing acetate formation from overflow metabolism in Escherichia coli : comparison of genetic engineering strategies to improve robustness toward sugar gradients in large-scale fermentation processes
ThesisA Novel Bio-Purification Process Employing an Engineered E. coli Strain for Downstream Processing of Lactic Acid Solutions from the Fermentation of Agro-Industrial by-Products
Nastouli, Alexandra; Moschona, Alexandra; Bizirtsakis, Panagiotis A. et al. · 2024 · Technical University of Denmark (DTU) · DOI
Full title: A Novel Bio-Purification Process Employing an Engineered E. coli Strain for Downstream Processing of Lactic Acid Solutions from the Fermentation of Agro-Industrial by-Products
ThesisSynthetic expression system enhances recombinant protein production in Aspergillus oryzae
van der Luijt, Casper Robert Balten; Rekdal, Vayu Maini; Chen, Yan et al. · 2024 · Technical University of Denmark (DTU) · DOI
Full title: Synthetic expression system enhances recombinant protein production in Aspergillus oryzae
Authors: van der Luijt, Casper Robert Balten; Rekdal, Vayu Maini; Chen, Yan; J. Petzold, Christopher; Keasling, Jay; Jahn, Leonie; Sommer, Morten Otto Alexander; id_orcid 0000-0003-4005-5674
ThesisAutomated Electrochemical Glucose Biosensor Platform as an Efficient Tool Toward On-Line Fermentation Monitoring:Novel Application Approaches and Insights
Pontius, Katrin; Semenova, Daria; Silina, Yuliya E. et al. · 2020 · Technical University of Denmark (DTU) · DOI
Full title: Automated Electrochemical Glucose Biosensor Platform as an Efficient Tool Toward On-Line Fermentation Monitoring:Novel Application Approaches and Insights
ThesisProduction of enzymatically active recombinant full-length barley high pI alpha-glucosidase of glycoside family 31 by high cell-density fermentation of Pichia pastoris and affinity purification
Næsted, Henrik; Kramhøft, Birte; Lok, F. et al. · 2006 · Technical University of Denmark (DTU) · DOI
Full title: Production of enzymatically active recombinant full-length barley high pI alpha-glucosidase of glycoside family 31 by high cell-density fermentation of Pichia pastoris and affinity purification
ThesisEstablishing Debaryomyces hansenii as a superior cell factory for the green transition: optimizing the use of industrial side-streams and complex feedstock for biotech applications
Duran, Mònica Estrada; id_orcid 0000-0002-8377-249X · 2024 · Technical University of Denmark (DTU) · DOI
Full title: Establishing Debaryomyces hansenii as a superior cell factory for the green transition: optimizing the use of industrial side-streams and complex feedstock for biotech applications
ThesisOpen (non-sterile) cultivations of Debaryomyces hansenii for recombinant protein production combining industrial side-streams with high salt content
Estrada, Mònica; id_orcid 0000-0002-8377-249X; Navarrete, Clara et al. · 2023 · Technical University of Denmark (DTU) · DOI
Full title: Open (non-sterile) cultivations of Debaryomyces hansenii for recombinant protein production combining industrial side-streams with high salt content
ThesisIntegrated Optimization of Hemoprotein Biomanufacturing in Yeast:From Upstream Engineering of Heme Synthesis to Downstream Purification of Human Hemoglobin
Frost, August Thinggaard · 2023 · Technical University of Denmark (DTU) · DOI
Full title: Integrated Optimization of Hemoprotein Biomanufacturing in Yeast:From Upstream Engineering of Heme Synthesis to Downstream Purification of Human Hemoglobin
ThesisBio-succinic acid production, up to pilot scale, by fermentation of industrial candy waste with Actinobacillus succinogenes 130Z and its downstream purification process
Lithourgidis, Antonios A.; Kotsopoulos, Thomas A.; Kalamaras, Sotirios D. et al. · 2023 · Technical University of Denmark (DTU) · DOI
Full title: Bio-succinic acid production, up to pilot scale, by fermentation of industrial candy waste with Actinobacillus succinogenes 130Z and its downstream purification process
ThesisDynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica
Kerssemakers, Abraham Antonius Johannes; id_orcid 0000-0002-3462-4642; Øzmerih, Süleyman et al. · 2023 · Technical University of Denmark (DTU) · DOI
Full title: Dynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica
Authors: Kerssemakers, Abraham Antonius Johannes; id_orcid 0000-0002-3462-4642; Øzmerih, Süleyman; Sin, Gürkan; id_orcid 0000-0003-0513-4502; Sudarsan, Suresh
ThesisA strategy for successful dual-species protein expression of genes with non-optimal codon usage destined for bacterial and yeast cell factories
Wäneskog, Marcus; Rasmussen, Trine Bertram; Jensen, Emil D. et al. · 2024 · Technical University of Denmark (DTU) · DOI
Full title: A strategy for successful dual-species protein expression of genes with non-optimal codon usage destined for bacterial and yeast cell factories
ThesisSynthetic expression system enhances recombinant protein production in Aspergillus oryzae
van der Luijt, Casper Robert Balten; Rekdal, Vayu Maini; Chen, Yan et al. · 2024 · Technical University of Denmark (DTU) · DOI
Full title: Synthetic expression system enhances recombinant protein production in Aspergillus oryzae
Authors: van der Luijt, Casper Robert Balten; Rekdal, Vayu Maini; Chen, Yan; J. Petzold, Christopher; Keasling, Jay; Jahn, Leonie; Sommer, Morten Otto Alexander; id_orcid 0000-0003-4005-5674
ThesisUntargeted GC-MS Metabolomics Reveals Changes in the Metabolite Dynamics of Industrial Scale Batch Fermentations of Streptoccoccus thermophilus Broth
Khakimov, Bekzod; Christiansen, Lene D.; Heins, Anna-Lena et al. · 2017 · Technical University of Denmark (DTU) · DOI
Full title: Untargeted GC-MS Metabolomics Reveals Changes in the Metabolite Dynamics of Industrial Scale Batch Fermentations of Streptoccoccus thermophilus Broth
Authors: Khakimov, Bekzod; Christiansen, Lene D.; Heins, Anna-Lena; Sørensen, Klavs M.; Schöller, Charlotte Elisabeth Grüner; Clausen, Anders; Skov, Thomas; Gernaey, Krist V.; id_orcid 0000-0002-0364-1773; Engelsen, Søren B.
ThesisRecombinant β-Carotene Production by Yarrowia lipolytica - Assessing the Potential of Micro-Scale Fermentation Analysis in Cell Factory Design and Bioreaction Optimization
Jacobsen, Irene Hjorth; Ledesma-Amaro, Rodrigo; Martinez, José Luis et al. · 2020 · Technical University of Denmark (DTU) · DOI
Full title: Recombinant β-Carotene Production by Yarrowia lipolytica - Assessing the Potential of Micro-Scale Fermentation Analysis in Cell Factory Design and Bioreaction Optimization
ThesisFunctional and structural evaluation of the antileukaemic enzyme l-asparaginase II expressed at low temperature by different Escherichia coli strains
de Moura, Werner Alfinito Feio [UNESP]; Schultz, Leonardo [UNESP]; Breyer, Carlos Alexandre [UNESP] et al. · 2020 · Universidade Estadual Paulista (UNESP) · DOI
Full title: Functional and structural evaluation of the antileukaemic enzyme l-asparaginase II expressed at low temperature by different Escherichia coli strains
Authors: de Moura, Werner Alfinito Feio [UNESP]; Schultz, Leonardo [UNESP]; Breyer, Carlos Alexandre [UNESP]; de Oliveira, Ana Laura Pires [UNESP]; Tairum, Carlos Abrunhosa [UNESP]; Fernandes, Gabriella Costa [UNESP]; Toyama, Marcos Hikari [UNESP]; Pessoa-Jr, Adalberto; Monteiro, Gisele; de Oliveira, Marcos Antonio [UNESP]
ThesisA Collagenolytic Aspartic Protease from Thermomucor indicae-seudaticae Expressed in Escherichia coli and Pichia pastoris
Pereira, Waldir Eduardo Simioni [UNESP]; da Silva, Ronivaldo Rodrigues [UNESP]; de Amo, Gabriela Salvador et al. · 2020 · Universidade Estadual Paulista (UNESP) · DOI
Full title: A Collagenolytic Aspartic Protease from Thermomucor indicae-seudaticae Expressed in Escherichia coli and Pichia pastoris
Authors: Pereira, Waldir Eduardo Simioni [UNESP]; da Silva, Ronivaldo Rodrigues [UNESP]; de Amo, Gabriela Salvador; Ruller, Roberto; Kishi, Luciano Takeshi; Boscolo, Maurício [UNESP]; Gomes, Eleni [UNESP]; da Silva, Roberto [UNESP]
ThesisHeterologous expression of GH11 xylanase from Myceliophthora heterothallica F.2.1.4 in Pichia pastoris
de Amo, Gabriela Salvador; Bezerra-Bussoli, Carolina [UNESP]; Rodrigues da Silva, Ronivaldo [UNESP] et al. · 2025 · Universidade Estadual Paulista (UNESP) · DOI
Full title: Heterologous expression of GH11 xylanase from Myceliophthora heterothallica F.2.1.4 in Pichia pastoris
Authors: de Amo, Gabriela Salvador; Bezerra-Bussoli, Carolina [UNESP]; Rodrigues da Silva, Ronivaldo [UNESP]; Kishi, Luciano Takeshi; Ferreira, Henrique [UNESP]; Gomes, Eleni [UNESP]; Bonilla-Rodriguez, Gustavo Orlando [UNESP]
ThesisMetabolic engineering of E. coli for pyocyanin production
Silva, Adilson Jose da; Cunha, Josivan de Souza; Hreha, Teri et al. · 2021 · Universidade Estadual Paulista (UNESP) · DOI
Full title: Metabolic engineering of E. coli for pyocyanin production
Authors: Silva, Adilson Jose da; Cunha, Josivan de Souza; Hreha, Teri; Micocci, Kelli Cristina [UNESP]; Selistre-de-Araujo, Heloisa Sobreiro; Barquera, Blanca; Koffas, Mattheos A. G.
ThesismiRNA heterologous production in bacteria: A systematic review focusing on the choice of plasmid features and bacterial/prokaryotic microfactory
da Silva Nonato, Nyelson; Nunes, Leandro Silva; da Silveira Martins, Amanda Weege et al. · 2025 · Universidade Estadual Paulista (UNESP) · DOI
Full title: miRNA heterologous production in bacteria: A systematic review focusing on the choice of plasmid features and bacterial/prokaryotic microfactory
Authors: da Silva Nonato, Nyelson; Nunes, Leandro Silva; da Silveira Martins, Amanda Weege; Pinhal, Danillo [UNESP]; Domingues, William Borges; Bellido-Quispe, Dionet Keny; Remião, Mariana Härter; Campos, Vinicius Farias
ThesisRecombinant xylanase production by Escherichia coli using a non-induced expression system with different nutrient sources
Mendonca, Elenira H. M.; Avanci, Nilton Cesar; Romano, Luis Henrique et al. · 2020 · Universidade Estadual Paulista (UNESP) · DOI
Full title: Recombinant xylanase production by Escherichia coli using a non-induced expression system with different nutrient sources
Authors: Mendonca, Elenira H. M.; Avanci, Nilton Cesar; Romano, Luis Henrique; Branco, Daniel Lopes; Padua, Alessandra Xavier de; Ward, Richard John; Baptista Neto, Alvaro de [UNESP]; Lourenzoni, Marcos Roberto
ThesisApplication of methylotrophic yeast Pichia pastoris in the field of food industry - A review
Silva, Viviane C. [UNESP]; Peres, Maristela F. S. [UNESP]; Gattas, Edwil Aparecida de Lucca [UNESP] · 2014 · Universidade Estadual Paulista (UNESP) · DOI
Full title: Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review
Authors: Silva, Viviane C. [UNESP]; Peres, Maristela F. S. [UNESP]; Gattas, Edwil Aparecida de Lucca [UNESP]
ThesisTrends in Enzyme Production from Citrus By-Products
Lima, Caio A. [UNESP]; Contato, Alex G.; de Oliveira, Fernanda et al. · 2025 · Universidade Estadual Paulista (UNESP) · DOI
Full title: Trends in Enzyme Production from Citrus By-Products
Authors: Lima, Caio A. [UNESP]; Contato, Alex G.; de Oliveira, Fernanda; da Silva, Silvio S.; Hidalgo, Vitor B.; Irfan, Muhammad; Gambarato, Bruno C.; Carvalho, Ana K. F.; Bento, Heitor B. S. [UNESP]
ThesisHyper-production of porcine contagious pleuropneumonia subunit vaccine proteins in Escherichia coli by developing a bicistronic T7 expression system
Sun, Manman; Gao, Alex Xiong; Li, An et al. · 2024 · Hong Kong University of Science and Technology (HKUST) · DOI
Full title: Hyper-production of porcine contagious pleuropneumonia subunit vaccine proteins in Escherichia coli by developing a bicistronic T7 expression system
ThesisA ubiquitous amino acid source for prokaryotic and eukaryotic cell-free transcription-translation systems
Nagappa, Lakshmeesha K; Sato, Wakana; Alam, Farzana et al. · 2022 · Imperial College London · DOI
Full title: A ubiquitous amino acid source for prokaryotic and eukaryotic cell-free transcription-translation systems
Authors: Nagappa, Lakshmeesha K; Sato, Wakana; Alam, Farzana; Chengan, Kameshwari; Smales, Christopher M; Von Der Haar, Tobias; Polizzi, Karen M; Adamala, Katarzyna P; Moore, Simon J
ThesisProduction and purification of an active CRM197 in Pichia pastoris and its immunological characterization using a Vi-typhoid antigen vaccine
Aw, Rochelle; Ashik, Muktadir R; Islam, Ariful AZM et al. · 2022 · Imperial College London · DOI
Full title: Production and purification of an active CRM197 in Pichia pastoris and its immunological characterization using a Vi-typhoid antigen vaccine
Authors: Aw, Rochelle; Ashik, Muktadir R; Islam, Ariful AZM; Khan, Imran; Mainuddin, Mohammad; Islam, Md Ashraful; Ahasan, Mohammad M; Polizzi, Karen M
ThesisRecombinant β-carotene production by yarrowia lipolytica - assessing the potential of micro-scale fermentation analysis in cell factory design and bioreaction optimization
Jacobsen, Irene Hjorth; Ledesma-Amaro, Rodrigo; Martinez, José Luis · 2020 · Imperial College London · DOI
Full title: Recombinant β-carotene production by yarrowia lipolytica - assessing the potential of micro-scale fermentation analysis in cell factory design and bioreaction optimization
Authors: Jacobsen, Irene Hjorth; Ledesma-Amaro, Rodrigo; Martinez, José Luis
ThesisAccelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform
Sawatzki, Annina; Hans, Sebastian; Narayanan, Harini et al. · 2018 · ETH Zurich (Swiss Federal Institute of Technology in Zurich) · DOI
Full title: Accelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform
ThesisLate Microaerobic Growth for Efficient Production of Human Cytochrome P450 3A4 in E. coli
Marchetti, Luca; Planchestainer, Matteo; id_orcid0000-0002-9938-5451 et al. · 2023 · ETH Zurich (Swiss Federal Institute of Technology in Zurich) · DOI
Full title: Late Microaerobic Growth for Efficient Production of Human Cytochrome P450 3A4 in E. coli
ThesisIn vivo production of a novel glycoconjugate vaccine against Shigella flexneri 2a in recombinant Escherichia coli: identification of stimulating factors for in vivo glycosylation
Kämpf, Michael M.; Braun, Martin; Sirena, Dominique et al. · 2015 · ETH Zurich (Swiss Federal Institute of Technology in Zurich) · DOI
Full title: In vivo production of a novel glycoconjugate vaccine against Shigella flexneri 2a in recombinant Escherichia coli: identification of stimulating factors for in vivo glycosylation
Authors: Kämpf, Michael M.; Braun, Martin; Sirena, Dominique; Ihssen, Julian; Thöny-Meyer, Linda; Ren, Qun
Year: 2015
Venue: ETH Zurich (Swiss Federal Institute of Technology in Zurich)
ThesisEstratègies d'operació en el procés de producció de proteïnes heteròlogues en Pichia pastoris: aplicació de tècniques de monitorització i control
Ramon Real, Ramon · 2011 · Universitat Autònoma de Barcelona (TDX) · DOI
Full title: Estratègies d'operació en el procés de producció de proteïnes heteròlogues en Pichia pastoris: aplicació de tècniques de monitorització i control
ThesisProviding growth-decoupled strategies for recombinant protein production in Komagataella phaffii: from promoter identification to bioprocess scale-up
Bernat Camps, Núria · 2024 · Universitat Autònoma de Barcelona (TDX) · DOI
Full title: Providing growth-decoupled strategies for recombinant protein production in Komagataella phaffii: from promoter identification to bioprocess scale-up
Heterologous overexpression and preliminary antimicrobial activity test of salmocin M, a novel colicin M-like bacteriocin against Salmonella sp.
Ewelina Łojewska; T. Sakowicz; M. Korycka-Machała et al. · 2022
Full title: Heterologous overexpression and preliminary antimicrobial activity test of salmocin M, a novel colicin M-like bacteriocin against Salmonella sp.
Authors: Ewelina Łojewska; T. Sakowicz; M. Korycka-Machała; Tomasz Kowalczyk
Online monitoring of protein refolding in inclusion body processing using intrinsic fluorescence
Chika Linda Igwe; Don Fabian Müller; Florian Gisperg et al. · 2024
Full title: Online monitoring of protein refolding in inclusion body processing using intrinsic fluorescence
Authors: Chika Linda Igwe; Don Fabian Müller; Florian Gisperg; J. N. Pauk; Matthias Kierein; Mohamed Elshazly; Robert Klausser; Julian Kopp; Oliver Spadiut; Eva Přáda Brichtová
Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories: production of human alpha-galactosidase A
U. Unzueta; F. Vázquez; G. Accardi et al. · 2015
Full title: Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories: production of human alpha-galactosidase A
Authors: U. Unzueta; F. Vázquez; G. Accardi; Rosa Mendoza; Verónica Toledo-Rubio; M. Giuliani; F. Sannino; E. Parrilli; I. Abasolo; S. Schwartz; M. Tutino; A. Villaverde; J. Corchero; N. Ferrer-Miralles
Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation
Andreas Maccani; M. Hackl; C. Leitner et al. · 2014
Full title: Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation
Authors: Andreas Maccani; M. Hackl; C. Leitner; W. Steinfellner; A. Graf; N. Tatto; M. Karbiener; M. Scheideler; J. Grillari; D. Mattanovich; R. Kunert; N. Borth; R. Grabherr; W. Ernst
Development of a constitutive and an auto-inducible high-yield expression system for recombinant protein production in the microalga Nannochloropsis oceanica.
de Grahl, Imke; Rout, Sweta Suman; Maple-Grødem, Jodi et al. · 2020
Full title: Development of a constitutive and an auto-inducible high-yield expression system for recombinant protein production in the microalga Nannochloropsis oceanica.
Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli
D. Wurm; Julian Quehenberger; Julia Mildner et al. · 2017
Full title: Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli
Authors: D. Wurm; Julian Quehenberger; Julia Mildner; B. Eggenreich; C. Slouka; A. Schwaighofer; K. Wieland; B. Lendl; Vignesh Rajamanickam; Christoph Herwig; O. Spadiut
Predictive approaches to guide the expression of recombinant vaccine targets in Escherichia coli: a case study presentation utilising Absynth Biologics Ltd. proprietary Clostridium difficile vaccine antigens.
Hussain, Hirra; McKenzie, Edward A; Robinson, Andrew M et al. · 2021
Full title: Predictive approaches to guide the expression of recombinant vaccine targets in Escherichia coli: a case study presentation utilising Absynth Biologics Ltd. proprietary Clostridium difficile vaccine antigens.
Authors: Hussain, Hirra; McKenzie, Edward A; Robinson, Andrew M; Gingles, Neill A; Marston, Fiona; Warwicker, Jim; Dickson, Alan J
Full title: Characterization of a novel + 70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry.
Phosphate-inducible poly-hydroxy butyrate production dynamics in CO2 supplemented upscaled cultivation of engineered Phaeodactylum tricornutum
Matthias Windhagauer; Raffaela M. Abbriano; Dorothea A. Pittrich et al. · 2022
Full title: Phosphate-inducible poly-hydroxy butyrate production dynamics in CO2 supplemented upscaled cultivation of engineered Phaeodactylum tricornutum
Authors: Matthias Windhagauer; Raffaela M. Abbriano; Dorothea A. Pittrich; M. Doblin
Characterization of Spent Yeast Streams from Precision Fermentations Driven by Genetically Engineered Yeasts: Valorization Potential for a Circular Bioeconomy
Full title: Characterization of Spent Yeast Streams from Precision Fermentations Driven by Genetically Engineered Yeasts: Valorization Potential for a Circular Bioeconomy
Authors: Deuchande, Teresa; Fundo, Joana F.; Rodrigues, Daniela; Pintado, Manuela; Amaro, Ana L.
Methanol addition after glucose depletion improves rPOXA 1B production under the pGap in P. pastoris X33: breaking the habit
Leidy D. Ardila-Leal; Raúl A. Poutou-Piñales; E. D. Morales-Álvarez et al. · 2021
Full title: Methanol addition after glucose depletion improves rPOXA 1B production under the pGap in P. pastoris X33: breaking the habit
Authors: Leidy D. Ardila-Leal; Raúl A. Poutou-Piñales; E. D. Morales-Álvarez; Claudia M Rivera-Hoyos; A. M. Pedroza-Rodríguez; Balkys E. Quevedo-Hidalgo; Alejandro Pérez-Flórez
Selection of CHO host and recombinant cell pools by inhibition of the proteasome results in enhanced product yields and cell specific productivity.
Knight, Tanya J; Turner, Sarah; Jaques, Colin M et al. · 2021
Full title: Selection of CHO host and recombinant cell pools by inhibition of the proteasome results in enhanced product yields and cell specific productivity.
Authors: Knight, Tanya J; Turner, Sarah; Jaques, Colin M; Smales, C Mark
Detection of antibodies against glucose 6-phosphate isomerase in synovial fluid of rheumatoid arthritis using surface plasmon resonance (BIAcore)
Ji-Yeon Kim; Mi-hong Lee; K. Jung et al. · 2003
Full title: Detection of antibodies against glucose 6-phosphate isomerase in synovial fluid of rheumatoid arthritis using surface plasmon resonance (BIAcore)
Authors: Ji-Yeon Kim; Mi-hong Lee; K. Jung; H. Na; H. Cha; Eun-Mi Ko; Tae Jin Kim
Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer
Denby, Charles M.; Li, Rachel A.; Vu, Van Tien et al. · 2018
Full title: Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer
Authors: Denby, Charles M.; Li, Rachel A.; Vu, Van Tien; Costello, Zak; Lin, Weiyin; Chan, Leanne Jade G.; Williams, Joseph C.; Donaldson, Bryan; Bamforth, Charles W.; Petzold, Christopher J.; Scheller, Henrik Vibe; Martín, Héctor García; Keasling, Jay D.
Multiplex secretome engineering enhances recombinant protein production and purity
S. Kol; Daniel Ley; T. Wulff et al. · 2019
Full title: Multiplex secretome engineering enhances recombinant protein production and purity
Authors: S. Kol; Daniel Ley; T. Wulff; Marianne Decker; Johnny Arnsdorf; J. Gutierrez; Austin W.T. Chiang; L. Pedersen; H. F. Kildegaard; G. Lee; N. Lewis
Combinatorial optimization of gene expression through recombinase-mediated promoter and terminator shuffling in yeast
Charlotte Cautereels; Jolien Smets; Peter Bircham et al. · 2024
Full title: Combinatorial optimization of gene expression through recombinase-mediated promoter and terminator shuffling in yeast
Authors: Charlotte Cautereels; Jolien Smets; Peter Bircham; Dries De Ruysscher; Anna Zimmermann; Peter De Rijk; Jan Steensels; Anton Gorkovskiy; J. Masschelein; K. Verstrepen
Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit
Rekdal, Vayu Maini; Luijt, Casper Robert Balten van der; Chen, Yan et al. · 2024
Full title: Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit
Authors: Rekdal, Vayu Maini; Luijt, Casper Robert Balten van der; Chen, Yan; Kakumanu, Ramu; Baidoo, Edward E. K.; Petzold, Christopher J.; Cruz‐Morales, Pablo; Keasling, Jay D.
Genomic and functional analyses of fungal and bacterial consortia that enable lignocellulose breakdown in goat gut microbiomes
Peng, Xuefeng; Wilken, St. Elmo; Lankiewicz, Thomas S. et al. · 2021
Full title: Genomic and functional analyses of fungal and bacterial consortia that enable lignocellulose breakdown in goat gut microbiomes
Authors: Peng, Xuefeng; Wilken, St. Elmo; Lankiewicz, Thomas S.; Gilmore, Sean; Brown, Jennifer L.; Henske, John K.; Swift, Candice L.; Salamov, Asaf; Barry, Kerrie; Grigoriev, Igor V.; Theodorou, Michael K.; Valentine, David L.; O’Malley, Michelle
Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans
R. Frandsen; Paiman Khorsand-Jamal; K. T. Kongstad et al. · 2018
Full title: Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans
Authors: R. Frandsen; Paiman Khorsand-Jamal; K. T. Kongstad; Majse Nafisi; R. Kannangara; D. Staerk; F. Okkels; K. Binderup; B. Madsen; B. Møller; U. Thrane; U. Mortensen
Crucial role of 4-deoxy-L-erythro-5-hexoseulose uronate reductase for alginate utilization revealed by adaptive evolution in engineered Saccharomyces cerevisiae
Fumiya Matsuoka; M. Hirayama; Takayuki Kashihara et al. · 2017
Full title: Crucial role of 4-deoxy-L-erythro-5-hexoseulose uronate reductase for alginate utilization revealed by adaptive evolution in engineered Saccharomyces cerevisiae
Authors: Fumiya Matsuoka; M. Hirayama; Takayuki Kashihara; Hideki Tanaka; W. Hashimoto; K. Murata; S. Kawai
A manually curated compendium of expression profiles for the microbial cell factory Corynebacterium glutamicum
Angela Kranz; T. Polen; Christian Kotulla et al. · 2022
Full title: A manually curated compendium of expression profiles for the microbial cell factory Corynebacterium glutamicum
Authors: Angela Kranz; T. Polen; Christian Kotulla; Annette Arndt; Graziella Bosco; Michael Bussmann; A. Chattopadhyay; A. Cramer; Cedric-Farhad Davoudi; Ursula Degner; Ramon Diesveld; Raphael Freiherr von Boeselager; Kim Gärtner; Cornelia Gätgens; T. Georgi; Christian Geraths; S. Haas; A. Heyer; Max Hünnefeld; T. Ishige; Armin Kabus; Nicolai Kallscheuer; Larissa Kever; Simon Klaffl; Britta Kleine; M. Kočan; A. Koch-Koerfges; K. Kraxner; Andreas Krug; Aileen Krüger; A. Küberl; Mohamed Labib; C. Lange; C. Mack; Tomoya Maeda; R. Mahr; Stephan Majda; Andrea Michel; Xenia Morosov; O. Müller; A. Nanda; J. Nickel; Jennifer Pahlke; E. Pfeifer; Laura Platzen; Paul Ramp; D. Rittmann; S. Schaffer; Sandra Scheele; Stephanie Spelberg; J. Schulte; J. Schweitzer; Georg Sindelar; Ulrike Sorger-Herrmann; Markus Spelberg; C. Stansen; Apilaasha Tharmasothirajan; J. V. Ooyen; Philana V van Summeren-Wesenhagen; M. Vogt; Sabrina Witthoff; Lingfeng Zhu; B. Eikmanns; M. Oldiges; Georg Schaumann; M. Baumgart; M. Brocker; L. Eggeling; R. Freudl; Julia Frunzke; Jan Marienhagen; V. Wendisch; M. Bott
Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Xiaowei Yu; Wei-Hong Sun; Ying-Zheng Wang et al. · 2017
Full title: Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Authors: Xiaowei Yu; Wei-Hong Sun; Ying-Zheng Wang; Yan Xu
Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells
C. R. Arbeitman; G. Auge; M. Blaustein et al. · 2020
Full title: Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells
Authors: C. R. Arbeitman; G. Auge; M. Blaustein; L. Bredeston; E. Corapi; P. Craig; L. A. Cossio; L. Dain; C. D’Alessio; Fernanda Elias; Natalia B Fernández; J. Gasulla; Natalia Gorojovsky; Gustavo E. Gudesblat; Maria Georgina Herrera; L. I. Ibañez; Tommy Idrovo; Matías Iglesias Randon; L. Kamenetzky; A. Nadra; D. Noseda; C. Pavan; M. Pavan; M. F. Pignataro; Ernesto A. Román; L. Ruberto; N. Rubinstein; Javier Santos; F. Duarte; A. Zelada
Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden
D. Wollborn; L. Munkler; Rebekka Horstmann et al. · 2022
Full title: Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden
Authors: D. Wollborn; L. Munkler; Rebekka Horstmann; Andrea Germer; L. Blank; J. Büchs
Artificial intelligence-based optimization for extracellular L-glutaminase free L-asparaginase production by Streptomyces violaceoruber under solid state fermentation conditions
Full title: Artificial intelligence-based optimization for extracellular L-glutaminase free L-asparaginase production by Streptomyces violaceoruber under solid state fermentation conditions
A galactose-based auto-expression system improves T7-inducible protein production in Escherichia coli
James Bosco; Emily Gagliano; Kassandra L Boshae et al. · 2025
Full title: A galactose-based auto-expression system improves T7-inducible protein production in Escherichia coli
Authors: James Bosco; Emily Gagliano; Kassandra L Boshae; John P. Statz; Timothy B Wheeler; DeAnna Cuello; Ashlyn Sliter; Christian Newby; Bernice Lin; Aysha Demeler; C. L. Pierpont; Cindee Yates-Hansen; M. Sydor; M. Ferrini; Kellie C Kuch; Brandon S. Cooper; Beverly J Piggott; Sarah J Certel; Kasper B. Hansen; Stephen R. Sprang; Bruce E. Bowler; Levi McClelland; M. Berkmen; Ekaterina Voronina
A self-inducible heterologous protein expression system in Escherichia coli
L. Briand; Guillaume Marcion; Guillaume Marcion et al. · 2016
Full title: A self-inducible heterologous protein expression system in Escherichia coli
Authors: L. Briand; Guillaume Marcion; Guillaume Marcion; Alexandre Kriznik; J. Heydel; Yves Artur; Carmen Garrido; Carmen Garrido; R. Seigneuric; R. Seigneuric; F. Neiers
N-hypermannose glycosylation disruption enhances recombinant protein production by regulating secretory pathway and cell wall integrity in Saccharomyces cerevisiae
Hongting Tang; Shenghuan Wang; Jiajing Wang et al. · 2016
Full title: N-hypermannose glycosylation disruption enhances recombinant protein production by regulating secretory pathway and cell wall integrity in Saccharomyces cerevisiae
Authors: Hongting Tang; Shenghuan Wang; Jiajing Wang; Meihui Song; Mengyang Xu; Mengying Zhang; Yu Shen; Jin Hou; X. Bao
Quantitative analysis of carbohydrate residues in dextran 40 from various sources: a comparative study using high-performance liquid chromatography coupled with a charged aerosol detector
Xie, Siwei; Jin, Zhuyu; Huang, Yan et al. · 2025
Full title: Quantitative analysis of carbohydrate residues in dextran 40 from various sources: a comparative study using high-performance liquid chromatography coupled with a charged aerosol detector
Green initiatives for the synthesis of polyamide monomers: precision fermentation using engineered Corynebacterium glutamicum and extraction of purified 5-aminovaleric acid (5AVA) and putrescine
Keerthi Sasikumar; Volker F. Wendisch; K. Nampoothiri · 2025
Full title: Green initiatives for the synthesis of polyamide monomers: precision fermentation using engineered Corynebacterium glutamicum and extraction of purified 5-aminovaleric acid (5AVA) and putrescine
Authors: Keerthi Sasikumar; Volker F. Wendisch; K. Nampoothiri
A genome-scale metabolic network model synergizes with statistical learning to predict amino acid concentrations in Chinese Hamster Ovary cell cultures
Song-Min Schinn; Carly Morrison; Wei Wei et al. · 2020
Full title: A genome-scale metabolic network model synergizes with statistical learning to predict amino acid concentrations in Chinese Hamster Ovary cell cultures
Authors: Song-Min Schinn; Carly Morrison; Wei Wei; Lin Zhang; Nathan E. Lewis
A High Throughput Assay for Measuring Secreted Protein Based on a de novo Fluorescent Reporter Reveals Regulatory and Structural Insights in Salmonella Type Three Secretion System.
Leach, Samuel Alexander; Summers, Jordan Scott; Wen, Edward et al. · 2025
Full title: A High Throughput Assay for Measuring Secreted Protein Based on a de novo Fluorescent Reporter Reveals Regulatory and Structural Insights in Salmonella Type Three Secretion System.
Authors: Leach, Samuel Alexander; Summers, Jordan Scott; Wen, Edward; Tullman-Ercek, Danielle
Synthetic ratio computation for programming population composition and multicellular morphology
B. An; Tzu-Chieh Tang; Qian Zhang et al. · 2025
Full title: Synthetic ratio computation for programming population composition and multicellular morphology
Authors: B. An; Tzu-Chieh Tang; Qian Zhang; Teng Wang; Yanyi Wang; Kesheng Gan; Kun Liu; Dan Zhang; Yuzhu Liu; Yukui Pan; Min Yu; William M. Shaw; Qianyi Liang; Yaomin Wang; Chunbo Lou; Timothy K. Lu; George M. Church; Chao Zhong
Natural allelic variations of <i>Saccharomyces cerevisiae</i> impact stuck fermentation due to the combined effect of ethanol and temperature; a QTL-mapping study
Marullo, Philippe; Durrens, Pascal; Peltier, Émilien et al. · 2019
Full title: Natural allelic variations of <i>Saccharomyces cerevisiae</i> impact stuck fermentation due to the combined effect of ethanol and temperature; a QTL-mapping study
Sustainable production of plastic-degrading enzymes in Chlamydomonas pacifica
C. Diaz; João Vitor Dutra Molino; Barbara Saucedo et al. · 2025
Full title: Sustainable production of plastic-degrading enzymes in Chlamydomonas pacifica
Authors: C. Diaz; João Vitor Dutra Molino; Barbara Saucedo; K. Kang; Évellin do Espirito Santo; Marissa Tessman; Abhishek Gupta; Michael D. Burkart; Ryan Simkovsky; S. Mayfield
Enhanced recombinant protein production and differential expression of molecular chaperones in sf-caspase-1-repressed stable cells after baculovirus infection.
Lai, Yiu-Kay; Hsu, John T-A; Chu, Chih-Chieh et al. · 2012
Full title: Enhanced recombinant protein production and differential expression of molecular chaperones in sf-caspase-1-repressed stable cells after baculovirus infection.
Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1
N. Ravin; M. Eldarov; V. Kadnikov et al. · 2013
Full title: Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1
Authors: N. Ravin; M. Eldarov; V. Kadnikov; A. Beletsky; J. Schneider; E. Mardanova; E. Smekalova; Maria I Zvereva; O. Dontsova; A. Mardanov; K. Skryabin
Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli
V. D. Nguyen; Feras Hatahet; Kirsi E. H. Salo et al. · 2011
Full title: Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli
Authors: V. D. Nguyen; Feras Hatahet; Kirsi E. H. Salo; Eveliina Enlund; Chi Zhang; L. Ruddock
Enhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch cultures.
Pilarek, Maciej; Glazyrina, Julia; Neubauer, Peter · 2011
Full title: Enhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch cultures.
Authors: Pilarek, Maciej; Glazyrina, Julia; Neubauer, Peter
Full title: Development of a cultivation process for the enhancement of human interferon alpha 2b production in the oleaginous yeast, Yarrowia lipolytica.
Full title: Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette.
Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures.
Vanz, Ana Letícia; Nimtz, Manfred; Rinas, Ursula · 2014
Full title: Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures.
Authors: Vanz, Ana Letícia; Nimtz, Manfred; Rinas, Ursula
Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: a review.
Cos, Oriol; Ramón, Ramón; Montesinos, José Luis et al. · 2006
Full title: Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: a review.
Authors: Cos, Oriol; Ramón, Ramón; Montesinos, José Luis; Valero, Francisco
Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis
Frick, Oliver; Wittmann, Christoph · 2005
Full title: Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis
The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies.
Lethanh, Ha; Neubauer, Peter; Hoffmann, Frank · 2005
Full title: The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant Escherichia coli and delay degradation of inclusion bodies.
Authors: Lethanh, Ha; Neubauer, Peter; Hoffmann, Frank
Identification of anthranilate and benzoate metabolic operons of Pseudomonas fluorescens and functional characterization of their promoter regions.
Retallack, Diane M; Thomas, Tracey C; Shao, Ying et al. · 2006
Full title: Identification of anthranilate and benzoate metabolic operons of Pseudomonas fluorescens and functional characterization of their promoter regions.
Authors: Retallack, Diane M; Thomas, Tracey C; Shao, Ying; Haney, Keith L; Resnick, Sol M; Lee, Vincent D; Squires, Charles H
Application of a wide-range yeast vector (CoMed) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts.
Steinborn, Gerhard; Böer, Erik; Scholz, Anja et al. · 2006
Full title: Application of a wide-range yeast vector (CoMed) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts.
Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen.
Gurramkonda, Chandrasekhar; Adnan, Ahmad; Gäbel, Thomas et al. · 2009
Full title: Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen.
Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime.
Holmes, William J; Darby, Richard Aj; Wilks, Martin Db et al. · 2009
Full title: Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime.
Authors: Holmes, William J; Darby, Richard Aj; Wilks, Martin Db; Smith, Rodney; Bill, Roslyn M
A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures.
Krause, Mirja; Ukkonen, Kaisa; Haataja, Tatu et al. · 2010
Full title: A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures.
Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels.
Carnicer, Marc; Baumann, Kristin; Töplitz, Isabelle et al. · 2009
Full title: Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels.
Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory.
Ferndahl, Cecilia; Bonander, Nicklas; Logez, Christel et al. · 2010
Full title: Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory.
Authors: Ferndahl, Cecilia; Bonander, Nicklas; Logez, Christel; Wagner, Renaud; Gustafsson, Lena; Larsson, Christer; Hedfalk, Kristina; Darby, Richard A J; Bill, Roslyn M
Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations.
Full title: Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations.
Differential affects of low glucose on the macroheterogeneity and microheterogeneity of glycosylation in CHO-EG2 camelid monoclonal antibodies
Bo Liu; Carina Villacres-Barragan; E. Lattová et al. · 2013
Full title: Differential affects of low glucose on the macroheterogeneity and microheterogeneity of glycosylation in CHO-EG2 camelid monoclonal antibodies
Authors: Bo Liu; Carina Villacres-Barragan; E. Lattová; M. Spearman; M. Butler
Eukaryotic protein production in designed storage organelles
M. Torrent; B. Llompart; Sabine Lasserre-Ramassamy et al. · 2009
Full title: Eukaryotic protein production in designed storage organelles
Authors: M. Torrent; B. Llompart; Sabine Lasserre-Ramassamy; I. Llop-Tous; M. Bastida; P. Marzábal; A. Westerholm-Parvinen; M. Saloheimo; P. Heifetz; M. Ludevid
Molecular characterization of cyclophilin A-like protein from Piriformospora indica for its potential role to abiotic stress tolerance in E. coli
D. Trivedi; M. W. Ansari; Tanima Dutta et al. · 2013
Full title: Molecular characterization of cyclophilin A-like protein from Piriformospora indica for its potential role to abiotic stress tolerance in E. coli
Authors: D. Trivedi; M. W. Ansari; Tanima Dutta; P. Singh; N. Tuteja
Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks.
Bawa, Zharain; Routledge, Sarah J; Jamshad, Mohammed et al. · 2014
Full title: Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks.
Authors: Bawa, Zharain; Routledge, Sarah J; Jamshad, Mohammed; Clare, Michelle; Sarkar, Debasmita; Dickerson, Ian; Ganzlin, Markus; Poyner, David R; Bill, Roslyn M
Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications.
Camattari, Andrea; Goh, Amelia; Yip, Lian Yee et al. · 2016
Full title: Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications.
The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli.
Krause, Mirja; Neubauer, Antje; Neubauer, Peter · 2016
Full title: The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli.
Authors: Krause, Mirja; Neubauer, Antje; Neubauer, Peter
Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization.
Schwarzhans, Jan-Philipp; Wibberg, Daniel; Winkler, Anika et al. · 2016
Full title: Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization.
Constitutively-stressed yeast strains are high-yielding for recombinant Fps1: implications for the translational regulation of an aquaporin.
Cartwright, Stephanie P; Darby, Richard A J; Sarkar, Debasmita et al. · 2017
Full title: Constitutively-stressed yeast strains are high-yielding for recombinant Fps1: implications for the translational regulation of an aquaporin.
Authors: Cartwright, Stephanie P; Darby, Richard A J; Sarkar, Debasmita; Bonander, Nicklas; Gross, Stephane R; Ashe, Mark P; Bill, Roslyn M
Metabolic and proteomic analyses of product selectivity and redox regulation in Clostridium pasteurianum grown on glycerol under varied iron availability
C. Groeger; Wei Wang; W. Sabra et al. · 2017
Full title: Metabolic and proteomic analyses of product selectivity and redox regulation in Clostridium pasteurianum grown on glycerol under varied iron availability
Authors: C. Groeger; Wei Wang; W. Sabra; T. Utesch; A. Zeng
Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker.
Betancur, Maritza Ocampo; Reis, Viviane Castelo Branco; Nicola, André Moraes et al. · 2017
Full title: Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker.
Authors: Betancur, Maritza Ocampo; Reis, Viviane Castelo Branco; Nicola, André Moraes; De Marco, Janice Lisboa; de Moraes, Lídia Maria Pepe; Torres, Fernando Araripe Gonçalves
Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.
Moser, Josef W; Prielhofer, Roland; Gerner, Samuel M et al. · 2017
Full title: Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.
Authors: Moser, Josef W; Prielhofer, Roland; Gerner, Samuel M; Graf, Alexandra B; Wilson, Iain B H; Mattanovich, Diethard; Dragosits, Martin
Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica.
Shrestha, Roshan P; Hildebrand, Mark · 2017
Full title: Development of a silicon limitation inducible expression system for recombinant protein production in the centric diatoms Thalassiosira pseudonana and Cyclotella cryptica.
Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks.
Valdez-Cruz, Norma A; Reynoso-Cereceda, Greta I; Pérez-Rodriguez, Saumel et al. · 2017
Full title: Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks.
RNA-guided single/double gene repressions in Corynebacterium glutamicum using an efficient CRISPR interference and its application to industrial strain.
Park, Jaehyun; Shin, Hyojung; Lee, Sun-Mi et al. · 2018
Full title: RNA-guided single/double gene repressions in Corynebacterium glutamicum using an efficient CRISPR interference and its application to industrial strain.
Authors: Park, Jaehyun; Shin, Hyojung; Lee, Sun-Mi; Um, Youngsoon; Woo, Han Min
Correction to: Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae.
Wehrs, Maren; Prahl, Jan-Philip; Moon, Jadie et al. · 2019
Full title: Correction to: Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae.
Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison.
Pekarsky, Alexander; Veiter, Lukas; Rajamanickam, Vignesh et al. · 2018
Full title: Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison.
Fate of the UPR marker protein Kar2/Bip and autophagic processes in fed-batch cultures of secretory insulin precursor producing Pichia pastoris.
Roth, Gustavo; Vanz, Ana Letícia; Lünsdorf, Heinrich et al. · 2018
Full title: Fate of the UPR marker protein Kar2/Bip and autophagic processes in fed-batch cultures of secretory insulin precursor producing Pichia pastoris.
Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production.
Gawin, Agnieszka; Peebo, Karl; Hans, Sebastian et al. · 2019
Full title: Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production.
Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris.
Marsalek, Lukas; Puxbaum, Verena; Buchetics, Markus et al. · 2019
Full title: Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris.
Engineering transcription factor-based biosensors for repressive regulation through transcriptional deactivation design in Saccharomyces cerevisiae.
Qiu, Chenxi; Chen, Xiaoxu; Rexida, Reheman et al. · 2020
Full title: Engineering transcription factor-based biosensors for repressive regulation through transcriptional deactivation design in Saccharomyces cerevisiae.
The signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host's fluorescent intensity.
Gao, Jianhua; Qian, Hongmei; Guo, Xiaoqin et al. · 2020
Full title: The signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host's fluorescent intensity.
Genome-scale metabolic modelling enables deciphering ethanol metabolism via the acrylate pathway in the propionate-producer Anaerotignum neopropionicum.
Benito-Vaquerizo, Sara; Parera Olm, Ivette; de Vroet, Thijs et al. · 2022
Full title: Genome-scale metabolic modelling enables deciphering ethanol metabolism via the acrylate pathway in the propionate-producer Anaerotignum neopropionicum.
Authors: Benito-Vaquerizo, Sara; Parera Olm, Ivette; de Vroet, Thijs; Schaap, Peter J; Sousa, Diana Z; Martins Dos Santos, Vitor A P; Suarez-Diez, Maria
Strain specific properties of Escherichia coli can prevent non-canonical amino acid misincorporation caused by scale-related process heterogeneities.
Mayer, Florian; Cserjan-Puschmann, Monika; Haslinger, Benedikt et al. · 2022
Full title: Strain specific properties of Escherichia coli can prevent non-canonical amino acid misincorporation caused by scale-related process heterogeneities.
Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography.
Carratalá, Jose Vicente; Atienza-Garriga, Jan; López-Laguna, Hèctor et al. · 2023
Full title: Enhanced recombinant protein capture, purity and yield from crude bacterial cell extracts by N-Lauroylsarcosine-assisted affinity chromatography.
Authors: Carratalá, Jose Vicente; Atienza-Garriga, Jan; López-Laguna, Hèctor; Vázquez, Esther; Villaverde, Antonio; Sánchez, Julieta M; Ferrer-Miralles, Neus
Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity
Guangle Yu; Xiaochen Li; Qiuyue Duan et al. · 2023
Full title: Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity
Authors: Guangle Yu; Xiaochen Li; Qiuyue Duan; Jun Fu; Youming Zhang; Hailong Wang; Ji Luan
A strategy to enhance and modify fatty acid synthesis in Corynebacterium glutamicum and Escherichia coli: overexpression of acyl-CoA thioesterases.
Liu, Jin; Mandlaa; Wang, Jia et al. · 2023
Full title: A strategy to enhance and modify fatty acid synthesis in Corynebacterium glutamicum and Escherichia coli: overexpression of acyl-CoA thioesterases.
RNA-seq reveals multifaceted gene expression response to Fab production in Escherichia coli fed-batch processes with particular focus on ribosome stalling.
Vazulka, Sophie; Schiavinato, Matteo; Tauer, Christopher et al. · 2024
Full title: RNA-seq reveals multifaceted gene expression response to Fab production in Escherichia coli fed-batch processes with particular focus on ribosome stalling.
Characterization and optimization of 5´ untranslated region containing poly-adenine tracts in Kluyveromyces marxianus using machine-learning model.
Zeng, Junyuan; Song, Kunfeng; Wang, Jingqi et al. · 2024
Full title: Characterization and optimization of 5´ untranslated region containing poly-adenine tracts in Kluyveromyces marxianus using machine-learning model.
Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source.
Arai, Toshiharu; Wada, Mayumi; Nishiguchi, Hiroki et al. · 2023
Full title: Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source.
Yields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study.
Arauzo-Aguilera, Klaudia; Buscajoni, Luisa; Koch, Karin et al. · 2023
Full title: Yields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study.
Modifications of the 5' region of the CASPONTM tag's mRNA further enhance soluble recombinant protein production in Escherichia coli.
Köppl, Christoph; Buchinger, Wolfgang; Striedner, Gerald et al. · 2024
Full title: Modifications of the 5' region of the CASPONTM tag's mRNA further enhance soluble recombinant protein production in Escherichia coli.
Correction to: Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid.
Àvila-Cabré, Sílvia; Pérez-Trujillo, Míriam; Albiol, Joan et al. · 2024
Full title: Correction to: Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid.
Comparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacterium glutamicum and leaky Escherichia coli in batch and fed-batch processes.
Fritzsche, Stefanie; Hübner, Holger; Oldiges, Marco et al. · 2024
Full title: Comparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacterium glutamicum and leaky Escherichia coli in batch and fed-batch processes.
No two clones are alike: characterization of heterologous subpopulations in a transgenic cell line of the model diatom Phaeodactylum tricornutum.
Diaz-Garza, Aracely Maribel; Merindol, Natacha; Dos Santos, Karen Cristine Gonçalves et al. · 2024
Full title: No two clones are alike: characterization of heterologous subpopulations in a transgenic cell line of the model diatom Phaeodactylum tricornutum.
Microbial lipases: advances in production, purification, biochemical characterization, and multifaceted applications in industry and medicine.
Abdelaziz, Ahmed A; Abo-Kamar, Amal M; Elkotb, Esraa Sobhy et al. · 2025
Full title: Microbial lipases: advances in production, purification, biochemical characterization, and multifaceted applications in industry and medicine.
Authors: Abdelaziz, Ahmed A; Abo-Kamar, Amal M; Elkotb, Esraa Sobhy; Al-Madboly, Lamiaa A
Enhanced L-tyrosine production in Bacillus amyloliquefaciens through alleviation of feedback Inhibition and coordinated pathway expression.
Zhao, Ziyue; Ji, Anying; Song, Can et al. · 2026
Full title: Enhanced L-tyrosine production in Bacillus amyloliquefaciens through alleviation of feedback Inhibition and coordinated pathway expression.
Model-driven exploration of lactose and galactose metabolism via an oxidoreductive pathway in Sungouiella intermedia for cell factory applications.
Peri, Kameshwara V R; Domenzain, Iván; Alalam, Hanna D et al. · 2026
Full title: Model-driven exploration of lactose and galactose metabolism via an oxidoreductive pathway in Sungouiella intermedia for cell factory applications.
Authors: Peri, Kameshwara V R; Domenzain, Iván; Alalam, Hanna D; Pianale, Luca Torello; Rascón, Abril Valverde; Nielsen, Jens; Geijer, Cecilia
Development of an efficient heterologous protein expression platform in Aspergillus niger through genetic modification of a glucoamylase hyperproducing industrial strain.
Gou, Fufan; Liu, Dandan; Gong, Chaohui et al. · 2025
Full title: Development of an efficient heterologous protein expression platform in Aspergillus niger through genetic modification of a glucoamylase hyperproducing industrial strain.
Metabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomer.
Sommer, Annalena; Pauli, Sarah; Kohlstedt, Michael et al. · 2026
Full title: Metabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomer.
Systematic perturbation of cultures of K. phaffii by carbon co-feeding show gene signatures associated with production of recombinant proteins.
Acharya, Raghav; Hinckley, Joshua; Barry, Rachel et al. · 2026
Full title: Systematic perturbation of cultures of K. phaffii by carbon co-feeding show gene signatures associated with production of recombinant proteins.
Significantly enhancing recombinant alkaline amylase production in Bacillus subtilis by integration of a novel mutagenesis-screening strategy with systems-level fermentation optimization
Yingfang Ma; W. Shen; Xianzhong Chen et al. · 2016
Full title: Significantly enhancing recombinant alkaline amylase production in Bacillus subtilis by integration of a novel mutagenesis-screening strategy with systems-level fermentation optimization
Authors: Yingfang Ma; W. Shen; Xianzhong Chen; Long Liu; Zhemin Zhou; Fei Xu; Haiquan Yang
Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum
Tian, Liang; Papanek, Beth; Olson, Daniel G. et al. · 2016
Full title: Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum
Authors: Tian, Liang; Papanek, Beth; Olson, Daniel G.; Rydzak, Thomas; Holwerda, Evert K.; Zheng, Tianyong; Zhou, Jilai; Maloney, Marybeth; Jiang, Nannan; Giannone, Richard J.; Hettich, Robert L.; Guss, Adam M.; Lynd, Lee R.
Effect of recombinant protein production and release on microalgal fitness and the impact of environmental conditions for localized therapeutic delivery.
Carvajal, Felipe; Vargas-Torres, Valentina; Becerra, Daniela et al. · 2025
Full title: Effect of recombinant protein production and release on microalgal fitness and the impact of environmental conditions for localized therapeutic delivery.
Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration.
Liu, Xin-Xin; Wang, Yao; Zhang, Jian-Hui et al. · 2024
Full title: Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration.
Lactic acid bacteria as microbial cell factories for the in vivo delivery of therapeutic proteins as secretable TAT fusion products
Giorgio Medici; G. Candini; Nicola Mottolese et al. · 2025
Full title: Lactic acid bacteria as microbial cell factories for the in vivo delivery of therapeutic proteins as secretable TAT fusion products
Authors: Giorgio Medici; G. Candini; Nicola Mottolese; B. Uguagliati; F. Trebbi; M. Loi; Angelica Marina Bove; S. Stojanov; Erika Esposito; Rosalba Vitagliano; Federica D'Amico; S. Turroni; Jessica Fiori; A. Berlec; S. Trazzi; Elisabetta Ciani
Engineered Escherichia coli platforms for tyrosine-derivative production from phenylalanine using phenylalanine hydroxylase and tetrahydrobiopterin-regeneration system
Y. Satoh; K. Fukui; D. Koma et al. · 2023
Full title: Engineered Escherichia coli platforms for tyrosine-derivative production from phenylalanine using phenylalanine hydroxylase and tetrahydrobiopterin-regeneration system
Authors: Y. Satoh; K. Fukui; D. Koma; Ning Shen; T. Lee
Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast.
Peng, Bingyin; Plan, Manuel R; Carpenter, Alexander et al. · 2017
Full title: Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast.
Authors: Peng, Bingyin; Plan, Manuel R; Carpenter, Alexander; Nielsen, Lars K; Vickers, Claudia E
Decreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris.
Jiao, Jian; Wang, Shuai; Tian, Hui et al. · 2020
Full title: Decreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris.
High-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterization
Martin Kangwa; Vikas Yelemane; A. N. Polat et al. · 2015
Full title: High-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterization
Authors: Martin Kangwa; Vikas Yelemane; A. N. Polat; K. D. D. Gorrepati; M. Grasselli; M. Fernández-Lahore
Process optimization for biosynthesis of pyruvate decarboxylase (PDC) and Neuberg’s ketol (PAC) from a novel Pichia cecembensis through response surface methodology
Mushtaq, Zareena; Mukhtar, Hamid · 2022
Full title: Process optimization for biosynthesis of pyruvate decarboxylase (PDC) and Neuberg’s ketol (PAC) from a novel Pichia cecembensis through response surface methodology
Correction: Activity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein Production.
Full title: Correction: Activity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein Production.
Analysis of 11,430 recombinant protein production experiments reveals that protein yield is tunable by synonymous codon changes of translation initiation sites.
Bhandari, Bikash K; Lim, Chun Shen; Remus, Daniela M et al. · 2021
Full title: Analysis of 11,430 recombinant protein production experiments reveals that protein yield is tunable by synonymous codon changes of translation initiation sites.
Authors: Bhandari, Bikash K; Lim, Chun Shen; Remus, Daniela M; Chen, Augustine; van Dolleweerd, Craig; Gardner, Paul P
Exploiting Nucleotide Composition to Engineer Promoters
M. Grabherr; Jens Pontiller; E. Mauceli et al. · 2011
Full title: Exploiting Nucleotide Composition to Engineer Promoters
Authors: M. Grabherr; Jens Pontiller; E. Mauceli; W. Ernst; Martina Baumann; T. Biagi; Ross Swofford; Pamela Russell; M. Zody; F. Di Palma; K. Lindblad-Toh; R. Grabherr
Full title: Activity of the Human Rhinovirus 3C Protease Studied in Various Buffers, Additives and Detergents Solutions for Recombinant Protein Production.
Evaluation of the recombinant protein Tp0965 of Treponema pallidum as candidate antigen for serological diagnosis of syphilis
A. Runina; Рунина Анастасия Владимировна; A. S. Starovoitova et al. · 2016
Full title: Evaluation of the recombinant protein Tp0965 of Treponema pallidum as candidate antigen for serological diagnosis of syphilis
Authors: A. Runina; Рунина Анастасия Владимировна; A. S. Starovoitova; Старовойтова Анастасия Сергеевна; D. Deryabin; Дерябин Дмитрий Геннадьевич; A. Kubanov; Кубанов Алексей Алексеевич
Comparative analysis of enzymatic activity of tannase in non-conventional yeasts to produce ellagic acid
Márquez-López, Anahí; Ramírez-Conejo, Juan David; Chávez-Parga, Ma. del Carmen et al. · 2019
Full title: Comparative analysis of enzymatic activity of tannase in non-conventional yeasts to produce ellagic acid
Authors: Márquez-López, Anahí; Ramírez-Conejo, Juan David; Chávez-Parga, Ma. del Carmen; Flores, Dora Cecilia Valencia; Jaramillo, Miguel Ángel Zamudio; Rodríguez, Horacio González; González‐Hernández, Juan Carlos
Potential of lignocellulolytic biocatalysts of native and proposed genetically engineered microbial cell factories on jute fiber modification and jute waste recycling: A review
Somnath Das; Dipankar Ghosh · 2022
Full title: Potential of lignocellulolytic biocatalysts of native and proposed genetically engineered microbial cell factories on jute fiber modification and jute waste recycling: A review
Process Design, Simulation, and Techno-Economic Evaluation of Recombinant Human Lactoferrin Production via Precision Fermentation in Pichia pastoris with Chromatography-Free Downstream Processing
Full title: Process Design, Simulation, and Techno-Economic Evaluation of Recombinant Human Lactoferrin Production via Precision Fermentation in Pichia pastoris with Chromatography-Free Downstream Processing
1P140 A novel expression system for high-level recombinant protein production in Escherichia coli(4. Protein engineering,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
E. Hojo; M. Kamiya; T. Aizawa et al. · 2006
Full title: 1P140 A novel expression system for high-level recombinant protein production in Escherichia coli(4. Protein engineering,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
Authors: E. Hojo; M. Kamiya; T. Aizawa; M. Miyazawa; Y. Kato; M. Demura; K. Kawano
Enhanced Acetate Tolerance and Recombinant Protein Accumulation in Escherichia coli by Transgenic Expression of a Heat Shock Protein from Carrot (Daucus carota L.)
Minhye Kim; Eun-Mi Im; Yeh Jin Ahn · 2022
Full title: Enhanced Acetate Tolerance and Recombinant Protein Accumulation in Escherichia coli by Transgenic Expression of a Heat Shock Protein from Carrot (Daucus carota L.)
The Use of Transcription Terminators to Generate Transgenic Lines of Chinese Hamster Ovary Cells (CHO) with Stable and High Level of Reporter Gene Expression.
Gasanov, N B; Toshchakov, S V; Georgiev, P G et al. · 2015
Full title: The Use of Transcription Terminators to Generate Transgenic Lines of Chinese Hamster Ovary Cells (CHO) with Stable and High Level of Reporter Gene Expression.
Authors: Gasanov, N B; Toshchakov, S V; Georgiev, P G; Maksimenko, O G
Selection of High Producers From Combinatorial Libraries for the Production of Recombinant Proteins in Escherichia coli and Vibrio natriegens
Joel Eichmann; M. Oberpaul; T. Weidner et al. · 2019
Full title: Selection of High Producers From Combinatorial Libraries for the Production of Recombinant Proteins in Escherichia coli and Vibrio natriegens
Authors: Joel Eichmann; M. Oberpaul; T. Weidner; Doreen Gerlach; P. Czermak
Metabolomic Alterations Do Not Induce Metabolic Burden in the Industrial Yeast M2n[pBKD2-Pccbgl1]-C1 Engineered by Multiple δ-Integration of a Fungal β-Glucosidase Gene
L. Favaro; L. Cagnin; L. Corte et al. · 2019
Full title: Metabolomic Alterations Do Not Induce Metabolic Burden in the Industrial Yeast M2n[pBKD2-Pccbgl1]-C1 Engineered by Multiple δ-Integration of a Fungal β-Glucosidase Gene
Authors: L. Favaro; L. Cagnin; L. Corte; L. Roscini; Fabio De Pascale; L. Treu; S. Campanaro; M. Basaglia; W. V. van Zyl; S. Casella; G. Cardinali
Membrane Modulation of Super-Secreting “midiBacillus” Expressing the Major Staphylococcus aureus Antigen – A Mass-Spectrometry-Based Absolute Quantification Approach
Minia Antelo-Varela; Rocío Aguilar Suárez; J. Bartel et al. · 2020
Full title: Membrane Modulation of Super-Secreting “midiBacillus” Expressing the Major Staphylococcus aureus Antigen – A Mass-Spectrometry-Based Absolute Quantification Approach
Authors: Minia Antelo-Varela; Rocío Aguilar Suárez; J. Bartel; Margarita Bernal-Cabas; T. Stobernack; Thomas Sura; J. V. van Dijl; S. Maaß; D. Becher
Global Reprogramming of Gene Transcription in Trichoderma reesei by Overexpressing an Artificial Transcription Factor for Improved Cellulase Production and Identification of Ypr1 as an Associated Regulator
Fei Zhang; Jia-Xiang Li; V. Champreda et al. · 2020
Full title: Global Reprogramming of Gene Transcription in Trichoderma reesei by Overexpressing an Artificial Transcription Factor for Improved Cellulase Production and Identification of Ypr1 as an Associated Regulator
Authors: Fei Zhang; Jia-Xiang Li; V. Champreda; Chen-Guang Liu; F. Bai; Xinqing Zhao
Reverse Engineering Targets for Recombinant Protein Production in Corynebacterium glutamicum Inspired by a Fast-Growing Evolved Descendant.
Lee, Min Ju; Park, Jihoon; Park, Kyunghoon et al. · 2020
Full title: Reverse Engineering Targets for Recombinant Protein Production in Corynebacterium glutamicum Inspired by a Fast-Growing Evolved Descendant.
Authors: Lee, Min Ju; Park, Jihoon; Park, Kyunghoon; Kim, Jihyun F; Kim, Pil
Microbial Cell Factory for Efficiently Synthesizing Plant Natural Products via Optimizing the Location and Adaptation of Pathway on Genome Scale
Bo Yang; Xudong Feng; Chun Li · 2020
Full title: Microbial Cell Factory for Efficiently Synthesizing Plant Natural Products via Optimizing the Location and Adaptation of Pathway on Genome Scale
Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production.
Gasset, Arnau; Garcia-Ortega, Xavier; Garrigós-Martínez, Javier et al. · 2022
Full title: Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production.
Authors: Gasset, Arnau; Garcia-Ortega, Xavier; Garrigós-Martínez, Javier; Valero, Francisco; Montesinos-Seguí, José Luis
Continuous Process Verification 4.0 application in upstream: adaptiveness implementation managed by AI in the hypoxic bioprocess of the Pichia pastoris cell factory.
Gasset, Arnau; Van Wijngaarden, Joeri; Mirabent, Ferran et al. · 2024
Full title: Continuous Process Verification 4.0 application in upstream: adaptiveness implementation managed by AI in the hypoxic bioprocess of the Pichia pastoris cell factory.
Authors: Gasset, Arnau; Van Wijngaarden, Joeri; Mirabent, Ferran; Sales-Vallverdú, Albert; Garcia-Ortega, Xavier; Montesinos-Seguí, José Luis; Manzano, Toni; Valero, Francisco
Editorial: Protein Aggregation and Solubility in Microorganisms (Archaea, Bacteria and Unicellular Eukaryotes): Implications and Applications.
Skretas, Georgios; Ventura, Salvador · 2020
Full title: Editorial: Protein Aggregation and Solubility in Microorganisms (Archaea, Bacteria and Unicellular Eukaryotes): Implications and Applications.
Whole Genome Sequencing Reveals Major Deletions in the Genome of M7, a Gamma Ray-Induced Mutant of Trichoderma virens That Is Repressed in Conidiation, Secondary Metabolism, and Mycoparasitism
S. Pachauri; P. D. Sherkhane; Vinay Kumar et al. · 2020
Full title: Whole Genome Sequencing Reveals Major Deletions in the Genome of M7, a Gamma Ray-Induced Mutant of Trichoderma virens That Is Repressed in Conidiation, Secondary Metabolism, and Mycoparasitism
Authors: S. Pachauri; P. D. Sherkhane; Vinay Kumar; P. Mukherjee
Transcriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a High-Cell-Density Fed-Batch Cultivation Strategy
Chengbo Zhang; Yu Ma; Huabiao Miao et al. · 2020
Full title: Transcriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a High-Cell-Density Fed-Batch Cultivation Strategy
Simultaneous determination of pentostatin and 2'-amino-2'-deoxyadenosine in fermentation broth by high performance liquid chromatography-tandem mass spectrometry
Zhao, Minmin; Zhang, Hongyu; Lou, Tingting et al. · 2021
Full title: Simultaneous determination of pentostatin and 2'-amino-2'-deoxyadenosine in fermentation broth by high performance liquid chromatography-tandem mass spectrometry
Gene expression, purification, and functional characterization of recombinant conotoxin μ-TIIIA and TIIIAlaMut in Escherichia coli with clinical evaluation of antiwrinkle efficacy.
Mikiewicz, Diana; Mazurkiewicz-Pisarek, Anna; Janczewska, Magdalena et al. · 2025
Full title: Gene expression, purification, and functional characterization of recombinant conotoxin μ-TIIIA and TIIIAlaMut in Escherichia coli with clinical evaluation of antiwrinkle efficacy.
Scale-Up Challenges and Optimization Strategies for Green Fluorescent Protein Production in E. coli JM109: A Shake Flask and Batch Fermentation Study
G. Miteu · 2026
Full title: Scale-Up Challenges and Optimization Strategies for Green Fluorescent Protein Production in E. coli JM109: A Shake Flask and Batch Fermentation Study
Simulation and techno-economic analysis of production processes of novel generation L-Asparaginases using recombinant Escherichia coli and Pichia pastoris
Azzoni, Adriano Rodrigues · 2022
Full title: Simulation and techno-economic analysis of production processes of novel generation L-Asparaginases using recombinant Escherichia coli and Pichia pastoris
Estratégias de indução, alimentação pós-indução e permeabilização de membrana em cultivo descontínuo alimentado de Escherichia coli BL21 (DE3) na produção de L-asparaginase II EcA (P40S/S206C)
Pessoa Junior, Adalberto · 2024
Full title: Estratégias de indução, alimentação pós-indução e permeabilização de membrana em cultivo descontínuo alimentado de Escherichia coli BL21 (DE3) na produção de L-asparaginase II EcA (P40S/S206C)
High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
Blanchard, Véronique; Gadkari, R.P; George, A.V.E. et al. · 2008
Full title: High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Dalvie, Neil C.; Lorgeree, Timothy R.; Yang, Yuchen et al. · 2024
Full title: CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Authors: Dalvie, Neil C.; Lorgeree, Timothy R.; Yang, Yuchen; Rodriguez-Aponte, Sergio A.; Whittaker, Charles A.; Hinckley, Joshua A.; Clark, John J.; Del Rosario, Amanda M.; Love, Kerry R.; Love, J. C.
Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain
Brady, Joseph R; Whittaker, Charles A; Tan, Melody C et al. · 2021
Full title: Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain
Authors: Brady, Joseph R; Whittaker, Charles A; Tan, Melody C; Kristensen, D Lee; Ma, Duanduan; Dalvie, Neil C; Love, Kerry Routenberg; Love, J Christopher
Adaptation of Aglycosylated Monoclonal Antibodies for Improved Production in Komagataella phaffii
Yang, Yuchen; Dalvie, Neil C; Brady, Joseph R et al. · 2025
Full title: Adaptation of Aglycosylated Monoclonal Antibodies for Improved Production in Komagataella phaffii
Authors: Yang, Yuchen; Dalvie, Neil C; Brady, Joseph R; Naranjo, Christopher A; Lorgeree, Timothy; Rodriguez‐Aponte, Sergio A; Johnston, Ryan S; Tracey, Mary K; Elenberger, Carmen M; Lee, Eric; Tié, Mark; Love, Kerry R; Love, J Christopher
Optimizing recombinant protein production in Chlamydomonas reinhardtii: turning a model organism into a commercially relevant biotechnological host
Torres-Tiji, Yasin · 2021
Full title: Optimizing recombinant protein production in Chlamydomonas reinhardtii: turning a model organism into a commercially relevant biotechnological host
Understanding the bug that makes our drugs: a multi-OMICs approach to engineer Escherichia coli for improved production of recombinant periplasmic proteins in the biopharmaceutical industry.
Seleiro, Alice Saville · 2022
Full title: Understanding the bug that makes our drugs: a multi-OMICs approach to engineer Escherichia coli for improved production of recombinant periplasmic proteins in the biopharmaceutical industry.
Eplt4 Proteinaceous Elicitor Produced in Pichia pastoris Has a Protective Effect Against Cercosporidium sofinum Infections of Soybean Leaves
Wang, Y; Song, J; Wu, Y et al. · 2021
Full title: Eplt4 Proteinaceous Elicitor Produced in Pichia pastoris Has a Protective Effect Against Cercosporidium sofinum Infections of Soybean Leaves
PERISCOPE-Opt: Machine learning-based prediction of optimal fermentation conditions and yields of recombinant periplasmic protein expressed in Escherichia coli
Packiam, KAR; Ooi, CW; Li, F et al. · 2022
Full title: PERISCOPE-Opt: Machine learning-based prediction of optimal fermentation conditions and yields of recombinant periplasmic protein expressed in Escherichia coli
Toward the production of milk-identical proteins using precision fermentation on agricultural waste substrates : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biological Sciences at Massey University, Albany, New Zealand
McIsaac, Emily Claire · 2023
Full title: Toward the production of milk-identical proteins using precision fermentation on agricultural waste substrates : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biological Sciences at Massey University, Albany, New Zealand
Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
Manderson, Daniel · 2017
Full title: Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
Simple surface plasmon resonance bio-sensor for the quantification of recombinant human epidermal growth factor (rhEGF) in expanded bed recovery
Faizal Wong, Fadzlie Wong · 2010
Full title: Simple surface plasmon resonance bio-sensor for the quantification of recombinant human epidermal growth factor (rhEGF) in expanded bed recovery
Fermentation strategies for production of hyaluronic acid through biosynthesis by streptococcus zooepidemicus and recombinant escherichia coli using stirred tank bioreactor
Lai, Zee Wei · 2013
Full title: Fermentation strategies for production of hyaluronic acid through biosynthesis by streptococcus zooepidemicus and recombinant escherichia coli using stirred tank bioreactor
Aumento de escala para a produção de Proteína verde fluorescente melhorada (Enhanced Green Fluorescent Protein - EGFP) a partir de Escherichia coli recombinante em biorreator convencional
Sousa, Ana Paula Abuchain [UNESP] · 2019
Full title: Aumento de escala para a produção de Proteína verde fluorescente melhorada (Enhanced Green Fluorescent Protein - EGFP) a partir de Escherichia coli recombinante em biorreator convencional
Escalamiento descendente del proceso de producción de proteína heteróloga por termo-inducción de cultivos de alta densidad celular de Escherichia coli : estudio de la respuesta transcripcional al choque térmico
Caspeta, Luis · 2021
Full title: Escalamiento descendente del proceso de producción de proteína heteróloga por termo-inducción de cultivos de alta densidad celular de Escherichia coli : estudio de la respuesta transcripcional al choque térmico
Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein
Green, Charlotte A; Kamble, Nitin S.; Court, Elizabeth K. et al. · 2019
Full title: Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein
Authors: Green, Charlotte A; Kamble, Nitin S.; Court, Elizabeth K.; Bryant, Owain J; Hicks, Matthew G.; Lennon, Christopher; Fraser, Gillian M; Wright, Phillip C; Stafford, Graham P
Full title: Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications.
L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic
Cámara Rey, Elena · 2017
Full title: L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic
Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess
Gasset Franch, Arnau · 2024
Full title: Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess
Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris
Nieto Taype, Miguel Angel · 2020
Full title: Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris
Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter
Barrigón de San Marcos, José Manuel · 2015
Full title: Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter
A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches
Garcia Ortega, Xavier · 2016
Full title: A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches
Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris
Garrigós Martínez, Javier · 2021
Full title: Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris