Host Cell Protein Clearance Across Multi Step Puri

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Paper Polysorbates degrading enzymes in biotherapeutics - a current status and future perspectives.

Felix, Marius Nicolaus; Waerner, Thomas; Lakatos, Daniel et al. · 2024 · Frontiers in bioengineering and biotechnology · DOI

Full title: Polysorbates degrading enzymes in biotherapeutics - a current status and future perspectives.

Authors: Felix, Marius Nicolaus; Waerner, Thomas; Lakatos, Daniel; Reisinger, Bernd; Fischer, Simon; Garidel, Patrick

Year: 2024

Venue: Frontiers in bioengineering and biotechnology

DOI: https://doi.org/10.3389/fbioe.2024.1490276

Paper Label-free quantification of host cell protein impurity in recombinant hemoglobin materials

Henrion, André; Arsene, Cristian; Liebl, Maik et al. · 2023 · Analytical and Bioanalytical Chemistry · DOI

Full title: Label-free quantification of host cell protein impurity in recombinant hemoglobin materials

Authors: Henrion, André; Arsene, Cristian; Liebl, Maik; O’Connor, Gavin

Year: 2023

Venue: Analytical and Bioanalytical Chemistry

DOI: https://doi.org/10.1007/s00216-023-05024-8

Paper Exploring sample preparation and data evaluation strategies for enhanced identification of host cell proteins in drug products of therapeutic antibodies and Fc-fusion proteins.

Esser-Skala, Wolfgang; Segl, Marius; Wohlschlager, Therese et al. · 2020 · Analytical and bioanalytical chemistry · DOI

Full title: Exploring sample preparation and data evaluation strategies for enhanced identification of host cell proteins in drug products of therapeutic antibodies and Fc-fusion proteins.

Authors: Esser-Skala, Wolfgang; Segl, Marius; Wohlschlager, Therese; Reisinger, Veronika; Holzmann, Johann; Huber, Christian G

Year: 2020

Venue: Analytical and bioanalytical chemistry

DOI: https://doi.org/10.1007/s00216-020-02796-1

Paper Monitoring process-related impurities in biologics-host cell protein analysis.

Pilely, Katrine; Johansen, Martin Rask; Lund, Rikke Raaen et al. · 2022 · Analytical and bioanalytical chemistry · DOI

Full title: Monitoring process-related impurities in biologics-host cell protein analysis.

Authors: Pilely, Katrine; Johansen, Martin Rask; Lund, Rikke Raaen; Kofoed, Thomas; Jørgensen, Thomas Kjærsgaard; Skriver, Lars; Mørtz, Ejvind

Year: 2022

Venue: Analytical and bioanalytical chemistry

DOI: https://doi.org/10.1007/s00216-021-03648-2

Paper Multiplex secretome engineering enhances recombinant protein production and purity.

Kol, Stefan; Ley, Daniel; Wulff, Tune et al. · 2020 · Nature communications · DOI

Full title: Multiplex secretome engineering enhances recombinant protein production and purity.

Authors: Kol, Stefan; Ley, Daniel; Wulff, Tune; Decker, Marianne; Arnsdorf, Johnny; Schoffelen, Sanne; Hansen, Anders Holmgaard; Jensen, Tanja Lyholm; Gutierrez, Jahir M; Chiang, Austin W T; Masson, Helen O; Palsson, Bernhard O; Voldborg, Bjørn G; Pedersen, Lasse Ebdrup; Kildegaard, Helene Faustrup; Lee, Gyun Min; Lewis, Nathan E

Year: 2020

Venue: Nature communications

DOI: https://doi.org/10.1038/s41467-020-15866-w

Paper Proteomic Analysis of Host Cell Protein Dynamics in the Culture Supernatants of Antibody-Producing CHO Cells.

Park, Jin Hyoung; Jin, Jong Hwa; Lim, Myung Sin et al. · 2017 · Scientific reports · DOI

Full title: Proteomic Analysis of Host Cell Protein Dynamics in the Culture Supernatants of Antibody-Producing CHO Cells.

Authors: Park, Jin Hyoung; Jin, Jong Hwa; Lim, Myung Sin; An, Hyun Joo; Kim, Jong Won; Lee, Gyun Min

Year: 2017

Venue: Scientific reports

DOI: https://doi.org/10.1038/srep44246

Paper Identification and characterization of a residual host cell protein hexosaminidase B associated with N-glycan degradation during the stability study of a therapeutic recombinant monoclonal antibody product.

Li, Xuanwen; An, Yan; Liao, Jing et al. · 2021 · Biotechnology progress · DOI

Full title: Identification and characterization of a residual host cell protein hexosaminidase B associated with N-glycan degradation during the stability study of a therapeutic recombinant monoclonal antibody product.

Authors: Li, Xuanwen; An, Yan; Liao, Jing; Xiao, Li; Swanson, Michael; Martinez-Fonts, Kirby; Pavon, Jorge Alexander; Sherer, Edward C; Jawa, Vibha; Wang, Fengqiang; Gao, Xinliu; Letarte, Simon; Richardson, Douglas D

Year: 2021

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.3128

Paper Definitive benchmarking of DDA and DIA for host cell protein analysis on the Orbitrap Astral in a regulatory-aligned framework

Khalil, Somar; Plisnier, Michel; Plisnier, Michel · 2025 · DOI

Full title: Definitive benchmarking of DDA and DIA for host cell protein analysis on the Orbitrap Astral in a regulatory-aligned framework

Authors: Khalil, Somar; Plisnier, Michel; Plisnier, Michel

Year: 2025

DOI: https://doi.org/10.1101/2025.07.31.667876

Paper Mass spectrometry-based investigation of measles and mumps virus proteome.

Sviben, Dora; Forcic, Dubravko; Halassy, Beata et al. · 2018 · Virology journal · DOI

Full title: Mass spectrometry-based investigation of measles and mumps virus proteome.

Authors: Sviben, Dora; Forcic, Dubravko; Halassy, Beata; Allmaier, Günter; Marchetti-Deschmann, Martina; Brgles, Marija

Year: 2018

Venue: Virology journal

DOI: https://doi.org/10.1186/s12985-018-1073-9

Paper Identification and Monitoring of Host Cell Proteins by Mass Spectrometry Combined with High Performance Immunochemistry Testing

Bomans, Katrin; Lang, Antje; Roedl, Veronika et al. · 2013 · PLoS ONE · DOI

Full title: Identification and Monitoring of Host Cell Proteins by Mass Spectrometry Combined with High Performance Immunochemistry Testing

Authors: Bomans, Katrin; Lang, Antje; Roedl, Veronika; Adolf, Lisa; Kyriosoglou, Kyrillos; Diepold, Katharina; Eberl, Gabriele; Mølhøj, Michael; Strauss, Ulrike; Schmalz, Christian; Vogel, Rudolf; Reusch, Dietmar; Wegele, Harald; Wiedmann, Michael; Bulau, Patrick

Year: 2013

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0081639

Paper Effective flow-through polishing strategies for knob-into-hole bispecific antibodies.

Chen, Serene W; Hoi, Kong Meng; Mahfut, Farouq Bin et al. · 2022 · Bioresources and bioprocessing · DOI

Full title: Effective flow-through polishing strategies for knob-into-hole bispecific antibodies.

Authors: Chen, Serene W; Hoi, Kong Meng; Mahfut, Farouq Bin; Yang, Yuansheng; Zhang, Wei

Year: 2022

Venue: Bioresources and bioprocessing

DOI: https://doi.org/10.1186/s40643-022-00590-8

Paper Immunoinformatic Risk Assessment of Host Cell Proteins During Process Development for Biologic Therapeutics.

Haltaufderhyde, Kirk; Roberts, Brian J; Khan, Sundos et al. · 2023 · The AAPS journal · DOI

Full title: Immunoinformatic Risk Assessment of Host Cell Proteins During Process Development for Biologic Therapeutics.

Authors: Haltaufderhyde, Kirk; Roberts, Brian J; Khan, Sundos; Terry, Frances; Boyle, Christine M; McAllister, Mitchell; Martin, William; Rosenberg, Amy; De Groot, Anne S

Year: 2023

Venue: The AAPS journal

DOI: https://doi.org/10.1208/s12248-023-00852-z

Paper Population balance modelling captures host cell protein dynamics in CHO cell cultures.

Alhuthali, Sakhr; Kontoravdi, Cleo · 2022 · PloS one · DOI

Full title: Population balance modelling captures host cell protein dynamics in CHO cell cultures.

Authors: Alhuthali, Sakhr; Kontoravdi, Cleo

Year: 2022

Venue: PloS one

DOI: https://doi.org/10.1371/journal.pone.0265886

Paper Assessment of the immunogenicity of residual host cell protein impurities of OsrHSA.

Abiri, Naghmeh; Pang, Jianlei; Ou, Jiquan et al. · 2018 · PloS one · DOI

Full title: Assessment of the immunogenicity of residual host cell protein impurities of OsrHSA.

Authors: Abiri, Naghmeh; Pang, Jianlei; Ou, Jiquan; Shi, Bo; Wang, Xianghong; Zhang, Sucai; Sun, Yunxia; Yang, Daichang

Year: 2018

Venue: PloS one

DOI: https://doi.org/10.1371/journal.pone.0193339

Paper Leveraging an advanced simulated moving bed approach to achieve 3-component separation for enhanced impurity removal in a non-affinity cation exchange capture step.

Chen, Serene W; Zheng, Zi Ying; Mahfut, Farouq Bin et al. · 2023 · PloS one · DOI

Full title: Leveraging an advanced simulated moving bed approach to achieve 3-component separation for enhanced impurity removal in a non-affinity cation exchange capture step.

Authors: Chen, Serene W; Zheng, Zi Ying; Mahfut, Farouq Bin; Yang, Yuansheng; Ogino, Masahiro; Okada, Kazuo; Sato, Kohei; Zhang, Wei

Year: 2023

Venue: PloS one

DOI: https://doi.org/10.1371/journal.pone.0280760

Paper Thermostable chaperone-based polypeptide biosynthesis: Enfuvirtide model product quality and protocol-related impurities.

Zenin, Vladimir; Tsedilin, Andrey; Yurkova, Maria et al. · 2023 · PloS one · DOI

Full title: Thermostable chaperone-based polypeptide biosynthesis: Enfuvirtide model product quality and protocol-related impurities.

Authors: Zenin, Vladimir; Tsedilin, Andrey; Yurkova, Maria; Siniavin, Andrey; Fedorov, Alexey

Year: 2023

Venue: PloS one

DOI: https://doi.org/10.1371/journal.pone.0286752

Paper Towards a cleaner CHO chassis: systematic knockout of host cell proteins for efficient biopharmaceutical manufacturing.

Marzluf, Jannis Peter; Klein, Jennifer; Kirchmeier, Daniela et al. · 2026 · Frontiers in bioengineering and biotechnology · DOI

Full title: Towards a cleaner CHO chassis: systematic knockout of host cell proteins for efficient biopharmaceutical manufacturing.

Authors: Marzluf, Jannis Peter; Klein, Jennifer; Kirchmeier, Daniela; Stärk, Alexander; Ziegner, Daniel; Gehrung, Markus; Rauh, Franziska; Rattay, Merle; Zehe, Christoph; Leroux, Ann-Cathrin

Year: 2026

Venue: Frontiers in bioengineering and biotechnology

DOI: https://doi.org/10.3389/fbioe.2026.1750646

Paper A comparison of SWATH-MS methods for measurement of residual host cell proteins in adeno-associated virus preparations.

Leibiger, Thomas M; Min, Lie; Lee, Kelvin H · 2025 · Frontiers in bioengineering and biotechnology · DOI

Full title: A comparison of SWATH-MS methods for measurement of residual host cell proteins in adeno-associated virus preparations.

Authors: Leibiger, Thomas M; Min, Lie; Lee, Kelvin H

Year: 2025

Venue: Frontiers in bioengineering and biotechnology

DOI: https://doi.org/10.3389/fbioe.2025.1579098

Paper Host Cell Proteins in Biologics Manufacturing: The Good, the Bad, and the Ugly.

Kornecki, Martin; Mestmäcker, Fabian; Zobel-Roos, Steffen et al. · 2017 · Antibodies (Basel, Switzerland) · DOI

Full title: Host Cell Proteins in Biologics Manufacturing: The Good, the Bad, and the Ugly.

Authors: Kornecki, Martin; Mestmäcker, Fabian; Zobel-Roos, Steffen; Heikaus de Figueiredo, Laura; Schlüter, Hartmut; Strube, Jochen

Year: 2017

Venue: Antibodies (Basel, Switzerland)

DOI: https://doi.org/10.3390/antib6030013

Paper A Combined Ultrafiltration/Diafiltration Step Facilitates the Purification of Cyanovirin-N From Transgenic Tobacco Extracts.

Opdensteinen, Patrick; Clodt, Juliana I; Müschen, Catherine R et al. · 2018 · Frontiers in bioengineering and biotechnology · DOI

Full title: A Combined Ultrafiltration/Diafiltration Step Facilitates the Purification of Cyanovirin-N From Transgenic Tobacco Extracts.

Authors: Opdensteinen, Patrick; Clodt, Juliana I; Müschen, Catherine R; Filiz, Volkan; Buyel, Johannes F

Year: 2018

Venue: Frontiers in bioengineering and biotechnology

DOI: https://doi.org/10.3389/fbioe.2018.00206

Paper Residual host cell proteins: sources, properties, detection methods and data acquisition modes.

Yao, Yifan; Wen, Xuemei; Pan, Hongjuan et al. · 2025 · Frontiers in microbiology · DOI

Full title: Residual host cell proteins: sources, properties, detection methods and data acquisition modes.

Authors: Yao, Yifan; Wen, Xuemei; Pan, Hongjuan; Chen, Ziwei

Year: 2025

Venue: Frontiers in microbiology

DOI: https://doi.org/10.3389/fmicb.2025.1658366

Paper Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines

S. Michalik; F. Siegerist; R. Palankar et al. · 2021 · Haematologica · DOI

Full title: Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines

Authors: S. Michalik; F. Siegerist; R. Palankar; K. Franzke; Maximilian Schindler; Alexander Reder; U. Seifert; Clemens Cammann; J. Wesche; L. Steil; Christian Hentschker; M. Gesell-Salazar; E. Reisinger; M. Beer; N. Endlich; A. Greinacher; U. Völker

Year: 2021

Venue: Haematologica

DOI: https://doi.org/10.3324/haematol.2021.280154

Paper Optimized Blanching Reduces the Host Cell Protein Content and Substantially Enhances the Recovery and Stability of Two Plant-Derived Malaria Vaccine Candidates.

Menzel, Stephan; Holland, Tanja; Boes, Alexander et al. · 2016 · Frontiers in plant science · DOI

Full title: Optimized Blanching Reduces the Host Cell Protein Content and Substantially Enhances the Recovery and Stability of Two Plant-Derived Malaria Vaccine Candidates.

Authors: Menzel, Stephan; Holland, Tanja; Boes, Alexander; Spiegel, Holger; Bolzenius, Johanna; Fischer, Rainer; Buyel, Johannes F

Year: 2016

Venue: Frontiers in plant science

DOI: https://doi.org/10.3389/fpls.2016.00159

Paper Optimization of Sequence, Display, and Mode of Operation of IgG-Binding Peptide Ligands to Develop Robust, High-Capacity Affinity Adsorbents That Afford High IgG Product Quality.

Islam, Tuhidul; Naik, Amith D; Hashimoto, Yasuhiro et al. · 2019 · International journal of molecular sciences · DOI

Full title: Optimization of Sequence, Display, and Mode of Operation of IgG-Binding Peptide Ligands to Develop Robust, High-Capacity Affinity Adsorbents That Afford High IgG Product Quality.

Authors: Islam, Tuhidul; Naik, Amith D; Hashimoto, Yasuhiro; Menegatti, Stefano; Carbonell, Ruben G

Year: 2019

Venue: International journal of molecular sciences

DOI: https://doi.org/10.3390/ijms20010161

Paper Site-Specific N-Glycosylation on the AAV8 Capsid Protein.

Aloor, Arya; Zhang, Junping; Gashash, Ebtesam A et al. · 2018 · Viruses · DOI

Full title: Site-Specific N-Glycosylation on the AAV8 Capsid Protein.

Authors: Aloor, Arya; Zhang, Junping; Gashash, Ebtesam A; Parameswaran, Aishwarya; Chrzanowski, Matthew; Ma, Cheng; Diao, Yong; Wang, Peng George; Xiao, Weidong

Year: 2018

Venue: Viruses

DOI: https://doi.org/10.3390/v10110644

Paper In-Line Monitoring of Downstream Purification Processes for VSV Based SARS-CoV-2 Vaccine Using a Novel Technique.

Makovitzki, Arik; Jayson, Avital; Oren, Ziv et al. · 2021 · Biotech (Basel (Switzerland)) · DOI

Full title: In-Line Monitoring of Downstream Purification Processes for VSV Based SARS-CoV-2 Vaccine Using a Novel Technique.

Authors: Makovitzki, Arik; Jayson, Avital; Oren, Ziv; Lerer, Elad; Kafri, Yaron; Dor, Eyal; Cherry, Lilach; Tzadok, Hanan; Levin, Lilach; Hazan, Ophir; Simon, Irit; Tal, Arnon; Girshengorn, Meni; Rosen, Osnat

Year: 2021

Venue: Biotech (Basel (Switzerland))

DOI: https://doi.org/10.3390/biotech10040025

Paper Nonwoven Ion-Exchange Membranes with High Protein Binding Capacity for Bioseparations.

Lemma, Solomon Mengistu; Boi, Cristiana; Carbonell, Ruben G · 2021 · Membranes · DOI

Full title: Nonwoven Ion-Exchange Membranes with High Protein Binding Capacity for Bioseparations.

Authors: Lemma, Solomon Mengistu; Boi, Cristiana; Carbonell, Ruben G

Year: 2021

Venue: Membranes

DOI: https://doi.org/10.3390/membranes11030181

Paper Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery.

Lavoie, R Ashton; di Fazio, Alice; Blackburn, R Kevin et al. · 2019 · International journal of molecular sciences · DOI

Full title: Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery.

Authors: Lavoie, R Ashton; di Fazio, Alice; Blackburn, R Kevin; Goshe, Michael B; Carbonell, Ruben G; Menegatti, Stefano

Year: 2019

Venue: International journal of molecular sciences

DOI: https://doi.org/10.3390/ijms20071729

Paper Multiplexed Competitive Screening of One-Bead-One-Component Combinatorial Libraries Using a ClonePix 2 Colony Sorter.

Lavoie, R Ashton; di Fazio, Alice; Carbonell, Ruben G et al. · 2019 · International journal of molecular sciences · DOI

Full title: Multiplexed Competitive Screening of One-Bead-One-Component Combinatorial Libraries Using a ClonePix 2 Colony Sorter.

Authors: Lavoie, R Ashton; di Fazio, Alice; Carbonell, Ruben G; Menegatti, Stefano

Year: 2019

Venue: International journal of molecular sciences

DOI: https://doi.org/10.3390/ijms20205119

Paper Applying UHPLC-HRAM MS/MS Method to Assess Host Cell Protein Clearance during the Purification Process Development of Therapeutic mAbs.

Kiyonami, Reiko; Melani, Rafael; Chen, Ying et al. · 2024 · International journal of molecular sciences · DOI

Full title: Applying UHPLC-HRAM MS/MS Method to Assess Host Cell Protein Clearance during the Purification Process Development of Therapeutic mAbs.

Authors: Kiyonami, Reiko; Melani, Rafael; Chen, Ying; Leon, A I De; Du, Min

Year: 2024

Venue: International journal of molecular sciences

DOI: https://doi.org/10.3390/ijms25179687

Paper A Single-Step Method for Harvesting Influenza Viral Particles from MDCK Cell Culture Supernatant with High Yield and Effective Impurity Removal.

Liu, Sixu; Li, Jingqi; Cheng, Qingtian et al. · 2024 · Viruses · DOI

Full title: A Single-Step Method for Harvesting Influenza Viral Particles from MDCK Cell Culture Supernatant with High Yield and Effective Impurity Removal.

Authors: Liu, Sixu; Li, Jingqi; Cheng, Qingtian; Duan, Kangyi; Wang, Zhan; Yan, Shuang; Tian, Shuaishuai; Wang, Hairui; Wu, Shaobin; Lei, Xinkui; Yang, Yu; Ma, Ningning

Year: 2024

Venue: Viruses

DOI: https://doi.org/10.3390/v16050768

Paper Scout-Triggered Multiple Reaction Monitoring Enables Robust Quantification of Host Cell Proteins Across Bioprocess Matrices.

Flecheux, Julie; Bardet, Chloé; Herment, Laura et al. · 2026 · Proteomes · DOI

Full title: Scout-Triggered Multiple Reaction Monitoring Enables Robust Quantification of Host Cell Proteins Across Bioprocess Matrices.

Authors: Flecheux, Julie; Bardet, Chloé; Herment, Laura; Fortin, Tanguy; Lemoine, Jérôme

Year: 2026

Venue: Proteomes

DOI: https://doi.org/10.3390/proteomes14010009

Paper Automated, Quantitative Capillary Western Blots to Analyze Host Cell Proteins in COVID-19 Vaccine Produced in Vero Cell Line.

Gillespie, Paul F; Wang, Yanjie; Yin, Kuo et al. · 2024 · Vaccines · DOI

Full title: Automated, Quantitative Capillary Western Blots to Analyze Host Cell Proteins in COVID-19 Vaccine Produced in Vero Cell Line.

Authors: Gillespie, Paul F; Wang, Yanjie; Yin, Kuo; Groegler, Emily; Cunningham, Nicholas; Stiving, Alyssa Q; Raffaele, Jessica; Marusa, Natalia; Tubbs, Christopher M; Loughney, John W; Winters, Michael A; Rustandi, Richard R

Year: 2024

Venue: Vaccines

DOI: https://doi.org/10.3390/vaccines12121373

Paper A Four-Step Purification Process for Gag VLPs: From Culture Supernatant to High-Purity Lyophilized Particles.

González-Domínguez, Irene; Lorenzo, Elianet; Bernier, Alice et al. · 2021 · Vaccines · DOI

Full title: A Four-Step Purification Process for Gag VLPs: From Culture Supernatant to High-Purity Lyophilized Particles.

Authors: González-Domínguez, Irene; Lorenzo, Elianet; Bernier, Alice; Cervera, Laura; Gòdia, Francesc; Kamen, Amine

Year: 2021

Venue: Vaccines

DOI: https://doi.org/10.3390/vaccines9101154

Paper Label-Free Quantitative Proteomics Analysis of COVID-19 Vaccines by Nano LC-HRMS.

Zhao, Hengzhi; Li, Wendong; Liu, Jingjing et al. · 2024 · Vaccines · DOI

Full title: Label-Free Quantitative Proteomics Analysis of COVID-19 Vaccines by Nano LC-HRMS.

Authors: Zhao, Hengzhi; Li, Wendong; Liu, Jingjing; Li, Xiao; Ji, Hong; Hu, Mo; Li, Min

Year: 2024

Venue: Vaccines

DOI: https://doi.org/10.3390/vaccines12091055

Paper Novel Standard Substance from Primary Hamster Kidney Cells for Quality Control of Human Rabies Vaccines in China.

Shi, Leitai; Li, Jia; Wu, Xiaohong et al. · 2025 · Vaccines · DOI

Full title: Novel Standard Substance from Primary Hamster Kidney Cells for Quality Control of Human Rabies Vaccines in China.

Authors: Shi, Leitai; Li, Jia; Wu, Xiaohong; Cao, Shouchun; Wang, Yunpeng; Zhao, Danhua; Li, Yuhua

Year: 2025

Venue: Vaccines

DOI: https://doi.org/10.3390/vaccines13020180

Paper Quantitation of Host Cell Proteins by Capillary LC/IMS/MS/MS in Combination with Rapid Digestion on Immobilized Trypsin Column Under Native Conditions.

Tomioka, Ryota; Ogata, Kosuke; Ishihama, Yasushi · 2024 · Mass spectrometry (Tokyo, Japan) · DOI

Full title: Quantitation of Host Cell Proteins by Capillary LC/IMS/MS/MS in Combination with Rapid Digestion on Immobilized Trypsin Column Under Native Conditions.

Authors: Tomioka, Ryota; Ogata, Kosuke; Ishihama, Yasushi

Year: 2024

Venue: Mass spectrometry (Tokyo, Japan)

DOI: https://doi.org/10.5702/massspectrometry.A0152

Thesis Prevention is better than cure : non-targeted and sensitive screening of impurities in plasma-derived immunoglobulin with mass spectrometry-based proteomics

Limonier, Franck · 2019 · Ghent University (Universiteit Gent) · DOI

Full title: Prevention is better than cure : non-targeted and sensitive screening of impurities in plasma-derived immunoglobulin with mass spectrometry-based proteomics

Authors: Limonier, Franck

Year: 2019

Venue: Ghent University (Universiteit Gent)

DOI: https://biblio.ugent.be/publication/8593810

Thesis Complex binding and elution behavior of therapeutic proteins under column overloading conditions

Seelinger, Felix · 2023 · Heidelberg University · DOI

Full title: Complex binding and elution behavior of therapeutic proteins under column overloading conditions

Authors: Seelinger, Felix

Year: 2023

Venue: Heidelberg University

DOI: https://archiv.ub.uni-heidelberg.de/volltextserver/32857/1/SeelingerDissertation.pdf

Thesis Mechanistic modeling and scale-up investigation of a preparative two-column purification step for a biopharmaceutical polypeptide

Koch, Jonas Martin Maria · 2024 · Heidelberg University · DOI

Full title: Mechanistic modeling and scale-up investigation of a preparative two-column purification step for a biopharmaceutical polypeptide

Authors: Koch, Jonas Martin Maria

Year: 2024

Venue: Heidelberg University

DOI: https://archiv.ub.uni-heidelberg.de/volltextserver/34272/1/Dissertation_Koch_Jonas.pdf

Thesis Untersuchung systematischer Zusammenhänge zwischen Chromatographie-Gel Eigenschaften und Antikörper-spezifischen Verunreinigungen in der Säulenchromatographie

Wittkopp, Felix · 2018 · Heidelberg University · DOI

Full title: Untersuchung systematischer Zusammenhänge zwischen Chromatographie-Gel Eigenschaften und Antikörper-spezifischen Verunreinigungen in der Säulenchromatographie

Authors: Wittkopp, Felix

Year: 2018

Venue: Heidelberg University

DOI: https://archiv.ub.uni-heidelberg.de/volltextserver/25163/1/Dissertation%20Felix%20Wittkopp%20heiDOC.pdf

Thesis Separation and characterization of antibody-based protein variants in column chromatography for the production of biopharmaceuticals

Sanchez Reyes, Gabriela · 2022 · Heidelberg University · DOI

Full title: Separation and characterization of antibody-based protein variants in column chromatography for the production of biopharmaceuticals

Authors: Sanchez Reyes, Gabriela

Year: 2022

Venue: Heidelberg University

DOI: https://archiv.ub.uni-heidelberg.de/volltextserver/30762/1/Dissertation_Sanchez%20Reyes%20Gabriela_FINAL.pdf

Thesis Production of biosimilar trastuzumab in plants: Expression, purification, and analysis of function

Grohs, Brittany Marie · 2020 · University of Guelph · DOI

Full title: Production of biosimilar trastuzumab in plants: Expression, purification, and analysis of function

Authors: Grohs, Brittany Marie

Year: 2020

Venue: University of Guelph

DOI: https://hdl.handle.net/10214/21780

Thesis Magnetic bead-based isolation of biological therapeutic modalities

Brechmann, Nils A. · 2022 · KTH Royal Institute of Technology · DOI

Full title: Magnetic bead-based isolation of biological therapeutic modalities

Authors: Brechmann, Nils A.

Year: 2022

Venue: KTH Royal Institute of Technology

DOI: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-321336

Thesis Strategies for facilitated protein recovery after recombinant production in Escherichia coli

Hedhammar, My · 2005 · KTH Royal Institute of Technology · DOI

Full title: Strategies for facilitated protein recovery after recombinant production in Escherichia coli

Authors: Hedhammar, My

Year: 2005

Venue: KTH Royal Institute of Technology

DOI: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-471

Thesis Calcium-dependent Affinity Domains for the Purification of Antibodies and Antibody Fragments

Scheffel, Julia · 2022 · KTH Royal Institute of Technology · DOI

Full title: Calcium-dependent Affinity Domains for the Purification of Antibodies and Antibody Fragments

Authors: Scheffel, Julia

Year: 2022

Venue: KTH Royal Institute of Technology

DOI: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-309572

Thesis Engineering Proteinaceous Ligands for Improved Performance in Affinity Chromatography Applications

Gülich, Susanne · 2002 · KTH Royal Institute of Technology · DOI

Full title: Engineering Proteinaceous Ligands for Improved Performance in Affinity Chromatography Applications

Authors: Gülich, Susanne

Year: 2002

Venue: KTH Royal Institute of Technology

DOI: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3327

Thesis Analysis of noncovalent and covalent protein-ligand complexes by electrospray ionisation mass spectrometry

Sundqvist, Gustav · 2008 · KTH Royal Institute of Technology · DOI

Full title: Analysis of noncovalent and covalent protein-ligand complexes by electrospray ionisation mass spectrometry

Authors: Sundqvist, Gustav

Year: 2008

Venue: KTH Royal Institute of Technology

DOI: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4728

Thesis Solid Substrate for High Throughput Protein Chromatography

Alvarez, Adrian · 2018 · University of California, San Diego (UCSD) · DOI

Full title: Solid Substrate for High Throughput Protein Chromatography

Authors: Alvarez, Adrian

Year: 2018

Venue: University of California, San Diego (UCSD)

DOI: https://escholarship.org/uc/item/7hd470qf

Thesis Process development for downstream processing of human growth hormone and its antagonist

Zheng, Yizhou · 1994 · OhioLINK Electronic Theses and Dissertations · DOI

Full title: Process development for downstream processing of human growth hormone and its antagonist

Authors: Zheng, Yizhou

Year: 1994

Venue: OhioLINK Electronic Theses and Dissertations

DOI: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1174330360

Thesis Příprava a charakterizace průtokových reaktoru pro validaci biologických léčiv

Kordík, Jakub · 2025 · Charles University Prague (Univerzita Karlova) · DOI

Full title: Příprava a charakterizace průtokových reaktoru pro validaci biologických léčiv

Authors: Kordík, Jakub

Year: 2025

Venue: Charles University Prague (Univerzita Karlova)

DOI: http://hdl.handle.net/20.500.11956/198905

Thesis Development of an integrated platform for the production of recombinant biopharmaceuticals

Segarra, Camille · 2013 · White Rose eTheses (Leeds, Sheffield, York) · DOI

Full title: Development of an integrated platform for the production of recombinant biopharmaceuticals

Authors: Segarra, Camille

Year: 2013

Venue: White Rose eTheses (Leeds, Sheffield, York)

DOI: https://etheses.whiterose.ac.uk/id/eprint/4147/1/Segarra_Thesis_PostCorrections.pdf

Thesis Evaluating Improvements and Challenges in Affinity Chromatography for AAV Purification

Dachenko, Alexandra · 2023 · Johns Hopkins University · DOI

Full title: Evaluating Improvements and Challenges in Affinity Chromatography for AAV Purification

Authors: Dachenko, Alexandra

Year: 2023

Venue: Johns Hopkins University

DOI: https://jscholarship.library.jhu.edu/handle/1774.2/69011

Thesis HARNESSING THE HIGH-AFFINITY INTERACTIONS BETWEEN SUPRAMOLECULAR POLYMERS AND PROTEINS FOR ANTIBODY SEPARATION AND LONG-ACTING RELEASE

Stern, David · 2022 · Johns Hopkins University · DOI

Full title: HARNESSING THE HIGH-AFFINITY INTERACTIONS BETWEEN SUPRAMOLECULAR POLYMERS AND PROTEINS FOR ANTIBODY SEPARATION AND LONG-ACTING RELEASE

Authors: Stern, David

Year: 2022

Venue: Johns Hopkins University

DOI: http://jhir.library.jhu.edu/handle/1774.2/67519

Thesis Bioprocess development: extraction and purification of human serum albumin from transgenic rice

Sheshukova, Kseniya Alekseyevna · 2019 · Kansas State University - K-REx · DOI

Full title: Bioprocess development: extraction and purification of human serum albumin from transgenic rice

Authors: Sheshukova, Kseniya Alekseyevna

Year: 2019

Venue: Kansas State University - K-REx

DOI: http://hdl.handle.net/2097/39681

Thesis Design and Control of Integrated Continuous Processes for the Purification of Biopharmaceuticals

Gomis Fons, Joaquin · 2022 · Lund University (LUP) · DOI

Full title: Design and Control of Integrated Continuous Processes for the Purification of Biopharmaceuticals

Authors: Gomis Fons, Joaquin

Year: 2022

Venue: Lund University (LUP)

DOI: https://lup.lub.lu.se/record/7de201f7-6a1b-4772-b3d1-e5d221e9515c

Thesis Modeling and Optimization of Reversed-Phase Chromatography : Effects of Modulators and Temperature

Arkell, Karolina · 2017 · Lund University (LUP) · DOI

Full title: Modeling and Optimization of Reversed-Phase Chromatography : Effects of Modulators and Temperature

Authors: Arkell, Karolina

Year: 2017

Venue: Lund University (LUP)

DOI: https://lup.lub.lu.se/record/145c9644-a818-4f5e-ab07-9a79be1655bc

Thesis Developing integrated downstream processes for next-generation biologics

Löfgren, Anton · 2020 · Lund University (LUP) · DOI

Full title: Developing integrated downstream processes for next-generation biologics

Authors: Löfgren, Anton

Year: 2020

Venue: Lund University (LUP)

DOI: https://lup.lub.lu.se/record/31772706-1f08-4ef5-b61e-db17291cd22b

Thesis Antibody-Fragments: Protein Engineering, Process Development, and their Application as Affinity Ligands

Sakhnini, Laila · 2019 · Lund University (LUP) · DOI

Full title: Antibody-Fragments: Protein Engineering, Process Development, and their Application as Affinity Ligands

Authors: Sakhnini, Laila

Year: 2019

Venue: Lund University (LUP)

DOI: https://lup.lub.lu.se/record/3156874d-3d8d-41ad-bd7a-6be6ffb8e037

Thesis Development and application of two-dimensional separation systems coupled to mass spectrometry incorporating capillary electrophoresis

Jooß, Kevin · 2019 · Technical University of Munich (TUM) · DOI

Full title: Development and application of two-dimensional separation systems coupled to mass spectrometry incorporating capillary electrophoresis

Authors: Jooß, Kevin

Year: 2019

Venue: Technical University of Munich (TUM)

DOI: https://mediatum.ub.tum.de/1469960

Thesis Monoclonal Antibody Production and Purification

McMahon, Hope E; Schwartz, Jessica W; Ray, Shritama · 2023 · University of Pennsylvania · DOI

Full title: Monoclonal Antibody Production and Purification

Authors: McMahon, Hope E; Schwartz, Jessica W; Ray, Shritama

Year: 2023

Venue: University of Pennsylvania

DOI: https://repository.upenn.edu/handle/20.500.14332/5925

Thesis Downstream purification and analysis of the recombinant human myelin basic protein produced in the milk of transgenic cows : a thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University (Palmerston North) New Zealand.

Al-Ghobashy, Medhat Ahmed Abdel-Hamid · 2010 · Massey University · DOI

Full title: Downstream purification and analysis of the recombinant human myelin basic protein produced in the milk of transgenic cows : a thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University (Palmerston North) New Zealand.

Authors: Al-Ghobashy, Medhat Ahmed Abdel-Hamid

Year: 2010

Venue: Massey University

DOI: http://hdl.handle.net/10179/1186

Thesis Statistical issues on mass spectrometry-based protein identification and quantitation

Liu, Shangbin · 2024 · University of Georgia - UGA Open Scholar · DOI

Full title: Statistical issues on mass spectrometry-based protein identification and quantitation

Authors: Liu, Shangbin

Year: 2024

Venue: University of Georgia - UGA Open Scholar

DOI: https://openscholar.uga.edu/record/19545/files/liu_shangbin_200812_phd.pdf

Thesis Membrane adsorbers and novel affinity peptides for recombinant protein purification

Weaver, Justin, author; Wickramasinghe, S. Ranil, advisor; Qian, Xianghong, committee member et al. · 2015 · Colorado State University · DOI

Full title: Membrane adsorbers and novel affinity peptides for recombinant protein purification

Authors: Weaver, Justin, author; Wickramasinghe, S. Ranil, advisor; Qian, Xianghong, committee member; Carlson, Jon O., committee member; Bailey, Travis S., committee member

Year: 2015

Venue: Colorado State University

DOI: http://hdl.handle.net/10217/166862

Thesis Multidimensional liquid chromatographic separations for proteomics

Burgess, Karl · 2008 · University of Glasgow · DOI

Full title: Multidimensional liquid chromatographic separations for proteomics

Authors: Burgess, Karl

Year: 2008

Venue: University of Glasgow

DOI: https://theses.gla.ac.uk/322/1/2008burgessphd.pdf

Thesis Purification of recombinant biopharmaceuticals by preparative electrophoresis

Catzel, Dallia · 2006 · UNSW Sydney · DOI

Full title: Purification of recombinant biopharmaceuticals by preparative electrophoresis

Authors: Catzel, Dallia

Year: 2006

Venue: UNSW Sydney

DOI: http://hdl.handle.net/1959.4/68595

Thesis Downstream processing of recombinant and endogenous proteins from livestock milk

Degener, Arthur W. Jr. · 2014 · Virginia Tech - VTechWorks · DOI

Full title: Downstream processing of recombinant and endogenous proteins from livestock milk

Authors: Degener, Arthur W. Jr.

Year: 2014

Venue: Virginia Tech - VTechWorks

DOI: http://hdl.handle.net/10919/27183

Thesis Identification of the factors impacting immunosorbent performance

Subramanian, Anuradha · 2014 · Virginia Tech - VTechWorks · DOI

Full title: Identification of the factors impacting immunosorbent performance

Authors: Subramanian, Anuradha

Year: 2014

Venue: Virginia Tech - VTechWorks

DOI: http://hdl.handle.net/10919/38450

Thesis Purification of the major envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions

Matanin, Brad Matthew · 2014 · Virginia Tech - VTechWorks · DOI

Full title: Purification of the major envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions

Authors: Matanin, Brad Matthew

Year: 2014

Venue: Virginia Tech - VTechWorks

DOI: http://hdl.handle.net/10919/33194

Thesis Purification of an acidic recombinant protein from transgenic tobacco

Holler, Christopher J. · 2014 · Virginia Tech - VTechWorks · DOI

Full title: Purification of an acidic recombinant protein from transgenic tobacco

Authors: Holler, Christopher J.

Year: 2014

Venue: Virginia Tech - VTechWorks

DOI: http://hdl.handle.net/10919/32379

Thesis Separation of Recombinant β-Glucuronidase from Transgenic Tobacco by Aqueous Two-Phase Extraction

Ross, Kristin Coby · 2014 · Virginia Tech - VTechWorks · DOI

Full title: Separation of Recombinant β-Glucuronidase from Transgenic Tobacco by Aqueous Two-Phase Extraction

Authors: Ross, Kristin Coby

Year: 2014

Venue: Virginia Tech - VTechWorks

DOI: http://hdl.handle.net/10919/43471

Thesis Evaluation of Phenomena that Determine the Performance of Immunoaffinity, Peptide-Based and Ion Exchange Affinity Sorbents

Sines, Brian James · 2014 · Virginia Tech - VTechWorks · DOI

Full title: Evaluation of Phenomena that Determine the Performance of Immunoaffinity, Peptide-Based and Ion Exchange Affinity Sorbents

Authors: Sines, Brian James

Year: 2014

Venue: Virginia Tech - VTechWorks

DOI: http://hdl.handle.net/10919/29841

Thesis Design of anion exchange cellulose hydrogel for large proteins

Kumar, Guneet · 2014 · Virginia Tech - VTechWorks · DOI

Full title: Design of anion exchange cellulose hydrogel for large proteins

Authors: Kumar, Guneet

Year: 2014

Venue: Virginia Tech - VTechWorks

DOI: http://hdl.handle.net/10919/38250

Thesis Antibody Purification from Tobacco by Protein A Affinity Chromatography

Hey, Carolyn McKenzie · 2014 · Virginia Tech - VTechWorks · DOI

Full title: Antibody Purification from Tobacco by Protein A Affinity Chromatography

Authors: Hey, Carolyn McKenzie

Year: 2014

Venue: Virginia Tech - VTechWorks

DOI: http://hdl.handle.net/10919/42645

Thesis Purification, formulation, and functionalization of HIV-1-based virus-like particles for vaccine and therapeutic applications

Lorenzo Romero, Elianet · 2024 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Purification, formulation, and functionalization of HIV-1-based virus-like particles for vaccine and therapeutic applications

Authors: Lorenzo Romero, Elianet

Year: 2024

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/692884

Thesis Developing and Establishing a Platform for the Automation of Monoclonal Antibody Drug Analysis

Federico, Joseph Vincent · 2019 · Johns Hopkins University · DOI

Full title: Developing and Establishing a Platform for the Automation of Monoclonal Antibody Drug Analysis

Authors: Federico, Joseph Vincent

Year: 2019

Venue: Johns Hopkins University

DOI: http://jhir.library.jhu.edu/handle/1774.2/60162

Thesis Improving Protein Detection Confidence Using SWATH-Mass Spectrometry with Large Peptide Reference Libraries

Wu, JX; Pascovici, D; Ignjatovic, V et al. · 2021 · University of Melbourne · DOI

Full title: Improving Protein Detection Confidence Using SWATH-Mass Spectrometry with Large Peptide Reference Libraries

Authors: Wu, JX; Pascovici, D; Ignjatovic, V; Song, X; Krisp, C; Molloy, MP

Year: 2021

Venue: University of Melbourne

DOI: https://hdl.handle.net/11343/293523

Thesis Measuring Hordein (Gluten) in Beer - A Comparison of ELISA and Mass Spectrometry

Tanner, GJ; Colgrave, ML; Blundell, MJ et al. · 2019 · University of Melbourne · DOI

Full title: Measuring Hordein (Gluten) in Beer - A Comparison of ELISA and Mass Spectrometry

Authors: Tanner, GJ; Colgrave, ML; Blundell, MJ; Goswami, HP; Howitt, CA

Year: 2019

Venue: University of Melbourne

DOI: https://hdl.handle.net/11343/224112

Thesis Application of mechanistic modelling in membrane and fiber chromatography for purification of biotherapeutics — A review

Qu, Y; Baker, I; Black, J et al. · 2024 · University of Melbourne · DOI

Full title: Application of mechanistic modelling in membrane and fiber chromatography for purification of biotherapeutics — A review

Authors: Qu, Y; Baker, I; Black, J; Fabri, L; Gras, SL; Lenhoff, AM; Kentish, SE

Year: 2024

Venue: University of Melbourne

DOI: https://hdl.handle.net/11343/340258

Thesis Protein A chromatography in monoclonal antibody purification

Curtis, Michael · 2018 · Kansas State University - K-REx · DOI

Full title: Protein A chromatography in monoclonal antibody purification

Authors: Curtis, Michael

Year: 2018

Venue: Kansas State University - K-REx

DOI: http://hdl.handle.net/2097/39284

Thesis Primary Recovery of Biologically Active Compounds Using Macroporous Monolithic Hydrogels

Hanora, Amro · 2005 · Lund University (LUP) · DOI

Full title: Primary Recovery of Biologically Active Compounds Using Macroporous Monolithic Hydrogels

Authors: Hanora, Amro

Year: 2005

Venue: Lund University (LUP)

DOI: https://lup.lub.lu.se/record/545390

Thesis Model Calibration and Optimization of a Protein Purification Process

Al-Kaisy, Sadiq · 2015 · Lund University Student Papers · DOI

Full title: Model Calibration and Optimization of a Protein Purification Process

Authors: Al-Kaisy, Sadiq

Year: 2015

Venue: Lund University Student Papers

DOI: http://lup.lub.lu.se/student-papers/record/5470040

Thesis Tools for digital twins in continuous downstream processing

Tallvod, Simon · 2023 · Lund University (LUP) · DOI

Full title: Tools for digital twins in continuous downstream processing

Authors: Tallvod, Simon

Year: 2023

Venue: Lund University (LUP)

DOI: https://lup.lub.lu.se/record/191aa4b8-990d-47f1-a248-68bfb57ff330

Thesis Modeling & Simulation of Affinity Chromatography & Investigation of CaptureSMB

Bogren, Dennis · 2018 · Lund University Student Papers · DOI

Full title: Modeling & Simulation of Affinity Chromatography & Investigation of CaptureSMB

Authors: Bogren, Dennis

Year: 2018

Venue: Lund University Student Papers

DOI: http://lup.lub.lu.se/student-papers/record/8951165

Thesis Iterative Learning Control for Preparative Chromatography

Espinoza, Daniel · 2020 · Lund University Student Papers · DOI

Full title: Iterative Learning Control for Preparative Chromatography

Authors: Espinoza, Daniel

Year: 2020

Venue: Lund University Student Papers

DOI: http://lup.lub.lu.se/student-papers/record/9007394

Thesis High throughput optimization of protein-A affinity chromatography for capture of humanized monoclonal antibody

Pakiman, Norazrina · 2012 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: High throughput optimization of protein-A affinity chromatography for capture of humanized monoclonal antibody

Authors: Pakiman, Norazrina

Year: 2012

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/48480/1/FK%202012%20123R.pdf

Thesis Development of dye affinity adsorbents for recovery of polyclonal anti-hepatitis B core antigen immunoglobulin G

Wongchuphan, Rattana · 2010 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Development of dye affinity adsorbents for recovery of polyclonal anti-hepatitis B core antigen immunoglobulin G

Authors: Wongchuphan, Rattana

Year: 2010

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/41141/1/FK%202010%2076R.pdf

Thesis CHARACTERIZATION AND QUANTITATION TECHNIQUES FOR IMMUNOGLOBULINS AND BIOTHERAPEUTIC DRUGS WITH MASS SPECTROMETRY

Fletcher, Tyler · 2024 · University of Georgia - UGA Open Scholar · DOI

Full title: CHARACTERIZATION AND QUANTITATION TECHNIQUES FOR IMMUNOGLOBULINS AND BIOTHERAPEUTIC DRUGS WITH MASS SPECTROMETRY

Authors: Fletcher, Tyler

Year: 2024

Venue: University of Georgia - UGA Open Scholar

DOI: https://openscholar.uga.edu/record/4525/files/FletcherTylerPHD.pdf

Thesis Purification and characterization of polyclonal anti-hepatitis B core antigen immunoglobulin G from rabbit serum

Sayed Hitam, Sharifah Mariam · 2016 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Purification and characterization of polyclonal anti-hepatitis B core antigen immunoglobulin G from rabbit serum

Authors: Sayed Hitam, Sharifah Mariam

Year: 2016

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/69717/1/IB%202016%207%20IR.pdf

Thesis Production and purification of nipah virus glycoprotein in Spodoptera frugiperda 9 (J.E. Smith) insect cell

Sunhare, Raksha · 2013 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Production and purification of nipah virus glycoprotein in Spodoptera frugiperda 9 (J.E. Smith) insect cell

Authors: Sunhare, Raksha

Year: 2013

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/40015/1/IB%202013%209.pdf

Thesis Direct Recovery of Recombinant Hepatitis B Core Antigen from Unclarified Escherichia Coli Feedstock Using Expanded Bed Adsorption Chromatography

Ng, Michelle Yeen Tan · 2008 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Direct Recovery of Recombinant Hepatitis B Core Antigen from Unclarified Escherichia Coli Feedstock Using Expanded Bed Adsorption Chromatography

Authors: Ng, Michelle Yeen Tan

Year: 2008

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/5392/1/IB_2008_5.pdf

Thesis Chromatographic Purification of Recombinant Nucleocapsid Protein of Nipah Virus from Escherichia Coli Homogenate

Chong, Fui Chin · 2010 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Chromatographic Purification of Recombinant Nucleocapsid Protein of Nipah Virus from Escherichia Coli Homogenate

Authors: Chong, Fui Chin

Year: 2010

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/7849/1/ABS_%3D%3D%3D%3D__FK_2010_3.pdf

Thesis Development Of An Integrated Recovery Process For Recombinant Hepatitis B Core Antigen

Ho, Chin Woi · 2007 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Development Of An Integrated Recovery Process For Recombinant Hepatitis B Core Antigen

Authors: Ho, Chin Woi

Year: 2007

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/5210/1/FK_2007_27.pdf

Thesis Partitioning behaviours and selective recovery of therapeutic protein in aqueous two-phase system

Chow, Yin Hui · 2016 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Partitioning behaviours and selective recovery of therapeutic protein in aqueous two-phase system

Authors: Chow, Yin Hui

Year: 2016

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/70219/1/FK%202016%2021%20T%20IR.pdf

Thesis The Influence Of M13 Bacteriophage On Protein Adsorption Using Anion Exchange Expanded Bed Chromatography

Mohamed @ Mahmood, Masniza · 2006 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: The Influence Of M13 Bacteriophage On Protein Adsorption Using Anion Exchange Expanded Bed Chromatography

Authors: Mohamed @ Mahmood, Masniza

Year: 2006

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/5162/1/FK_2006_108a.pdf

Thesis Data concerning the chromatographic isolation of bovine IgG from milk- and colostral whey

Heidebrecht, Hans-Jürgen; Kainz, Bernadette; Schopf, Roland et al. · 2018 · Utrecht University · DOI

Full title: Data concerning the chromatographic isolation of bovine IgG from milk- and colostral whey

Authors: Heidebrecht, Hans-Jürgen; Kainz, Bernadette; Schopf, Roland; Godl, Klaus; Karcier, Züleyha; Kulozik, Ulrich; Förster, Beatrix

Year: 2018

Venue: Utrecht University

DOI: https://dspace.library.uu.nl/handle/1874/384260

Thesis Isolation of biofunctional bovine immunoglobulin G from milk- and colostral whey with mixed-mode chromatography at lab and pilot scale

Heidebrecht, Hans-Jürgen; Kainz, Bernadette; Schopf, Roland et al. · 2018 · Utrecht University · DOI

Full title: Isolation of biofunctional bovine immunoglobulin G from milk- and colostral whey with mixed-mode chromatography at lab and pilot scale

Authors: Heidebrecht, Hans-Jürgen; Kainz, Bernadette; Schopf, Roland; Godl, Klaus; Karcier, Züleyha; Kulozik, Ulrich; Förster, Beatrix

Year: 2018

Venue: Utrecht University

DOI: https://dspace.library.uu.nl/handle/1874/384261

Thesis On-demand manufacturing of clinical-quality biopharmaceuticals

Crowell, Laura E; Lu, Amos E; Love, Kerry R et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI

Full title: On-demand manufacturing of clinical-quality biopharmaceuticals

Authors: Crowell, Laura E; Lu, Amos E; Love, Kerry R; Stockdale, Alan; Timmick, Steven M; Wu, Di; Wang, Yu Annie; Doherty, William; Bonnyman, Alexandra; Vecchiarello, Nicholas; Goodwine, Chaz; Bradbury, Lisa; Brady, Joseph R; Clark, John J; Colant, Noelle A; Cvetkovic, Aleksandar; Dalvie, Neil C; Liu, Diana; Liu, Yanjun; Mascarenhas, Craig A; Matthews, Catherine B; Mozdzierz, Nicholas J; Shah, Kartik A; Wu, Shiaw-Lin; Hancock, William S; Braatz, Richard D; Cramer, Steven M; Love, J Christopher

Year: 2021

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/134719

Thesis Rapid optimization of processes for the integrated purification of biopharmaceuticals

Crowell, Laura E; Rodriguez, Sergio A; Love, Kerry R et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Rapid optimization of processes for the integrated purification of biopharmaceuticals

Authors: Crowell, Laura E; Rodriguez, Sergio A; Love, Kerry R; Cramer, Steven M; Love, J Christopher

Year: 2021

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/135519

Thesis Minimal purification method enables developability assessment of recombinant proteins

Rodriguez‐Aponte, Sergio A; Naranjo, Christopher A; Johnston, Ryan S et al. · 2025 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Minimal purification method enables developability assessment of recombinant proteins

Authors: Rodriguez‐Aponte, Sergio A; Naranjo, Christopher A; Johnston, Ryan S; Dalvie, Neil C; Crowell, Laura E; Bajoria, Sakshi; Kumru, Ozan S; Joshi, Sangeeta B; Volkin, David B; Love, J Christopher

Year: 2025

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/163511

Thesis Understanding electrostatic interaction on strong cation-exchanger via co-ion valency effects

Essert, GM; de Souza, JP; Schwaminger, SP et al. · 2024 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Understanding electrostatic interaction on strong cation-exchanger via co-ion valency effects

Authors: Essert, GM; de Souza, JP; Schwaminger, SP; Bazant, MZ; Berensmeier, S

Year: 2024

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/157408

Thesis Large-Scale Purification and Characterization of Recombinant Receptor-Binding Domain (RBD) of SARS-CoV-2 Spike Protein Expressed in Yeast

Nagar, Gaurav; Jain, Siddharth; Rajurkar, Meghraj et al. · 2023 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Large-Scale Purification and Characterization of Recombinant Receptor-Binding Domain (RBD) of SARS-CoV-2 Spike Protein Expressed in Yeast

Authors: Nagar, Gaurav; Jain, Siddharth; Rajurkar, Meghraj; Lothe, Rakesh; Rao, Harish; Majumdar, Sourav; Gautam, Manish; Rodriguez-Aponte, Sergio A.; Crowell, Laura E.; Love, J. Christopher; Dandekar, Prajakta; Puranik, Amita; Gairola, Sunil; Shaligram, Umesh; Jain, Ratnesh

Year: 2023

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/152537

Thesis Development of a platform process for the production and purification of single‐domain antibodies

Crowell, Laura E; Goodwine, Chaz; Holt, Carla S et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Development of a platform process for the production and purification of single‐domain antibodies

Authors: Crowell, Laura E; Goodwine, Chaz; Holt, Carla S; Rocha, Lucia; Vega, Celina; Rodriguez, Sergio A; Dalvie, Neil C; Tracey, Mary K; Puntel, Mariana; Wigdorovitz, Andrés; Parreño, Viviana; Love, Kerry R; Cramer, Steven M; Love, J Christopher

Year: 2021

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/135524

Thesis Purification of recombinant proteins

Rutt, George Clifford · 2008 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Purification of recombinant proteins

Authors: Rutt, George Clifford

Year: 2008

Venue: Massachusetts Institute of Technology (MIT)

DOI: http://hdl.handle.net/1721.1/42614

Thesis Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Zhao, Lei; Whiteaker, Jeffrey R.; Pope, Matthew E. et al. · 2014 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Authors: Zhao, Lei; Whiteaker, Jeffrey R.; Pope, Matthew E.; Kuhn, Eric; Jackson, Angela; Anderson, N. Leigh; Pearson, Terry W.; Paulovich, Amanda G.; Carr, Steven A

Year: 2014

Venue: Massachusetts Institute of Technology (MIT)

DOI: http://hdl.handle.net/1721.1/89657

Thesis HIGH EFFICIENCY AFFINITY CAPTURE AND PURIFICATION OF IMMUNOGLOBULIN G USING MAGNETIC PARTICLES WITH BIOINSPIRIED PEPTIDE LIGANDS

Ou, Shih-Hao · 2019 · Johns Hopkins University · DOI

Full title: HIGH EFFICIENCY AFFINITY CAPTURE AND PURIFICATION OF IMMUNOGLOBULIN G USING MAGNETIC PARTICLES WITH BIOINSPIRIED PEPTIDE LIGANDS

Authors: Ou, Shih-Hao

Year: 2019

Venue: Johns Hopkins University

DOI: http://jhir.library.jhu.edu/handle/1774.2/61606

Thesis Resin Lifetime and Aging Behavior in Protein A Chromatography

Gooley, Sarah · 2024 · Johns Hopkins University · DOI

Full title: Resin Lifetime and Aging Behavior in Protein A Chromatography

Authors: Gooley, Sarah

Year: 2024

Venue: Johns Hopkins University

DOI: https://jscholarship.library.jhu.edu/handle/1774.2/69585

Thesis Insight into purification of monoclonal antibodies in industrial columns via studies of Protein A binding capacity by in situ ATR-FTIR spectroscopy

Beattie, james; Rowland-Jones, Ruth; Farys, Monika et al. · 2021 · Imperial College London · DOI

Full title: Insight into purification of monoclonal antibodies in industrial columns via studies of Protein A binding capacity by in situ ATR-FTIR spectroscopy

Authors: Beattie, james; Rowland-Jones, Ruth; Farys, Monika; Kucia-Tran, Richard; Kazarian, Sergei; Byrne, Bernadette

Year: 2021

Venue: Imperial College London

DOI: https://pubs.rsc.org/en/content/articlelanding/2021/AN/D1AN00985K

Thesis Characterization of the diffusive properties of the affinity chromatography system of Human Immunoglobulin-G and Protein A Sepharose

Dutta, Rañjita · 1991 · UNSW Sydney · DOI

Full title: Characterization of the diffusive properties of the affinity chromatography system of Human Immunoglobulin-G and Protein A Sepharose

Authors: Dutta, Rañjita

Year: 1991

Venue: UNSW Sydney

DOI: http://hdl.handle.net/1959.4/59597

Thesis Monoclonal Antibody Expression and Novel Purification in Nicotiana benthamiana

Fulton, Andrew Dale · 2014 · Virginia Tech - VTechWorks · DOI

Full title: Monoclonal Antibody Expression and Novel Purification in Nicotiana benthamiana

Authors: Fulton, Andrew Dale

Year: 2014

Venue: Virginia Tech - VTechWorks

DOI: http://hdl.handle.net/10919/43361

Thesis The development of a weak anion micro-capillary film for protein chromatography.

Kouyoumdjian, AJM; Lazar, RA; Slater, NKH · 2016 · University of Cambridge · DOI

Full title: The development of a weak anion micro-capillary film for protein chromatography.

Authors: Kouyoumdjian, AJM; Lazar, RA; Slater, NKH

Year: 2016

Venue: University of Cambridge

DOI: https://www.repository.cam.ac.uk/handle/1810/260967

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Metadata only

Paper Investigating the combination of single‐pass tangential flow filtration and anion exchange chromatography for intensified mAb polishing

Thomas Elich; E. Goodrich; H. Lutz et al. · 2019 · Biotechnology progress (Print) · DOI

Full title: Investigating the combination of single‐pass tangential flow filtration and anion exchange chromatography for intensified mAb polishing

Authors: Thomas Elich; E. Goodrich; H. Lutz; Ushma Mehta

Year: 2019

Venue: Biotechnology progress (Print)

DOI: https://doi.org/10.1002/btpr.2862

Paper Enhancing Protein A performance in mAb processing: A method to reduce and rapidly evaluate host cell DNA levels during primary clarification

Koehler, Kenneth Christopher; Jokondo, Zona; Narayan, Janani et al. · 2019 · Biotechnology Progress · DOI

Full title: Enhancing Protein A performance in mAb processing: A method to reduce and rapidly evaluate host cell DNA levels during primary clarification

Authors: Koehler, Kenneth Christopher; Jokondo, Zona; Narayan, Janani; Voloshin, Alexei; Castro‐Forero, Angelines A.

Year: 2019

Venue: Biotechnology Progress

DOI: https://doi.org/10.1002/btpr.2882

Paper The effect of feed quality due to clarification strategy on the design and performance of protein A periodic counter-current chromatography.

El-Sabbahy, Hani; Ward, David; Ogonah, Olotu et al. · 2018 · Biotechnology progress · DOI

Full title: The effect of feed quality due to clarification strategy on the design and performance of protein A periodic counter-current chromatography.

Authors: El-Sabbahy, Hani; Ward, David; Ogonah, Olotu; Deakin, Lynne; Jellum, Gregory M; Bracewell, Daniel G

Year: 2018

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.2709

Paper Effectiveness of host cell protein removal using depth filtration with a filter containing diatomaceous earth.

Nejatishahidein, Negin; Borujeni, Ehsan E; Roush, David J et al. · 2020 · Biotechnology progress · DOI

Full title: Effectiveness of host cell protein removal using depth filtration with a filter containing diatomaceous earth.

Authors: Nejatishahidein, Negin; Borujeni, Ehsan E; Roush, David J; Zydney, Andrew L

Year: 2020

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.3028

Paper Anxa2- and Ctsd-knockout CHO cell lines to diminish the risk of contamination with host cell proteins.

Fukuda, Nobuo; Senga, Yukako; Honda, Shinya · 2019 · Biotechnology progress · DOI

Full title: Anxa2- and Ctsd-knockout CHO cell lines to diminish the risk of contamination with host cell proteins.

Authors: Fukuda, Nobuo; Senga, Yukako; Honda, Shinya

Year: 2019

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.2820

Paper An innovative strategy to recycle permeate in biologics continuous manufacturing process to improve material efficiency and sustainability.

Madabhushi, Sri Ranganayaki; Huang, Chung-Jr; Wang, Xiaowen et al. · 2022 · Biotechnology progress · DOI

Full title: An innovative strategy to recycle permeate in biologics continuous manufacturing process to improve material efficiency and sustainability.

Authors: Madabhushi, Sri Ranganayaki; Huang, Chung-Jr; Wang, Xiaowen; Bui, Ashley; Atieh, Tariq Bassam; Rayfield, William J; Jayapal, Karthik P; Lin, Henry

Year: 2022

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.3262

Paper Identification of upstream culture conditions and harvest time parameters that affect host cell protein clearance.

Wilson, Louisa J; Lewis, Will; Kucia-Tran, Richard et al. · 2019 · Biotechnology progress · DOI

Full title: Identification of upstream culture conditions and harvest time parameters that affect host cell protein clearance.

Authors: Wilson, Louisa J; Lewis, Will; Kucia-Tran, Richard; Bracewell, Daniel G

Year: 2019

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.2805

Paper Development of an activated carbon filtration step and high throughput screening method to remove host cell proteins from a recombinant enzyme process.

Slocum, Ashley; Santora, Steven; Ly, Mellisa et al. · 2021 · Biotechnology progress · DOI

Full title: Development of an activated carbon filtration step and high throughput screening method to remove host cell proteins from a recombinant enzyme process.

Authors: Slocum, Ashley; Santora, Steven; Ly, Mellisa; Zhang, Junyan; Castano, Juan; Becerra-Arteaga, Alejandro

Year: 2021

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.3151

Paper Toward optimal clearance: A universal affinity-based mass spectrometry approach for comprehensive ELISA reagent coverage evaluation and HCP hitchhiker analysis.

Seisenberger, Christina; Graf, Tobias; Haindl, Markus et al. · 2022 · Biotechnology progress · DOI

Full title: Toward optimal clearance: A universal affinity-based mass spectrometry approach for comprehensive ELISA reagent coverage evaluation and HCP hitchhiker analysis.

Authors: Seisenberger, Christina; Graf, Tobias; Haindl, Markus; Wegele, Harald; Wiedmann, Michael; Wohlrab, Stefanie

Year: 2022

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.3244

Li, Xuanwen; An, Yan; Liao, Jing et al. · 2021 · Biotechnology progress · DOI

Year: 2021

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.3128

Paper Host cell proteins in monoclonal antibody processing: Control, detection, and removal.

Ito, Takao; Lutz, Herb; Tan, Lihan et al. · 2024 · Biotechnology progress · DOI

Full title: Host cell proteins in monoclonal antibody processing: Control, detection, and removal.

Authors: Ito, Takao; Lutz, Herb; Tan, Lihan; Wang, Bin; Tan, Janice; Patel, Masum; Chen, Lance; Tsunakawa, Yuki; Park, Byunghyun; Banerjee, Subhasis

Year: 2024

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.3448

Paper Depth filter material process interaction in the harvest of mammalian cells

Maria Parau; J. Pullen; D. Bracewell · 2023 · Biotechnology progress (Print) · DOI

Full title: Depth filter material process interaction in the harvest of mammalian cells

Authors: Maria Parau; J. Pullen; D. Bracewell

Year: 2023

Venue: Biotechnology progress (Print)

DOI: https://doi.org/10.1002/btpr.3329

Paper Proteomic analysis of host cell protein fouling during bioreactor harvesting.

Zhang, Da; Wickramasinghe, S Ranil; Zydney, Andrew L et al. · 2024 · Biotechnology progress · DOI

Full title: Proteomic analysis of host cell protein fouling during bioreactor harvesting.

Authors: Zhang, Da; Wickramasinghe, S Ranil; Zydney, Andrew L; Smelko, John P; Loman, Abdullah; Wheeler, April; Qian, Xianghong

Year: 2024

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.3453

Paper Proteomics‐based method to comprehensively model the removal of host cell protein impurities

Disela, Roxana; Keulen, Daphne; Fotou, Eleni et al. · 2024 · Biotechnology Progress · DOI

Full title: Proteomics‐based method to comprehensively model the removal of host cell protein impurities

Authors: Disela, Roxana; Keulen, Daphne; Fotou, Eleni; Neijenhuis, Tim; Bussy, Olivier Le; Geldhof, Geoffroy; Pabst, Martin; Ottens, Marcel

Year: 2024

Venue: Biotechnology Progress

DOI: https://doi.org/10.1002/btpr.3494

Paper Design and optimization of membrane chromatography for monoclonal antibody charge variant separation.

Nadar, Sathish; Somasundaram, Balaji; Charry, Marcela et al. · 2022 · Biotechnology progress · DOI

Full title: Design and optimization of membrane chromatography for monoclonal antibody charge variant separation.

Authors: Nadar, Sathish; Somasundaram, Balaji; Charry, Marcela; Billakanti, Jagan; Shave, Evan; Baker, Kym; Lua, Linda H L

Year: 2022

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.3288

Paper Methods comparison of two-dimensional gel electrophoresis for host cell protein characterization.

King, Abigail; Zhao, Yiwei; Lazar, Alexandru et al. · 2024 · Biotechnology progress · DOI

Full title: Methods comparison of two-dimensional gel electrophoresis for host cell protein characterization.

Authors: King, Abigail; Zhao, Yiwei; Lazar, Alexandru; Capron, Margeaux; Thiruvur, Niranjan; Liu, Xinrong

Year: 2024

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.3452

Paper Use of LC-MS to characterize host cell protein removal during depth filtration.

Chu, Liang-Kai; Borujeni, Ehsan Espah; Xu, Xuankuo et al. · 2025 · Biotechnology progress · DOI

Full title: Use of LC-MS to characterize host cell protein removal during depth filtration.

Authors: Chu, Liang-Kai; Borujeni, Ehsan Espah; Xu, Xuankuo; Zydney, Andrew L

Year: 2025

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.70044

Paper Monoclonal antibody purification using cationic polyelectrolytes: an alternative to column chromatography.

Peram, Thanmaya; McDonald, Paul; Carter-Franklin, Jayme et al. · 2010 · Biotechnology progress · DOI

Full title: Monoclonal antibody purification using cationic polyelectrolytes: an alternative to column chromatography.

Authors: Peram, Thanmaya; McDonald, Paul; Carter-Franklin, Jayme; Fahrner, Robert

Year: 2010

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.437

Paper Host cell protein clearance during protein A chromatography: development of an improved column wash step.

Shukla, Abhinav A; Hinckley, Peter · 2008 · Biotechnology progress · DOI

Full title: Host cell protein clearance during protein A chromatography: development of an improved column wash step.

Authors: Shukla, Abhinav A; Hinckley, Peter

Year: 2008

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.50

Paper Establishment and evaluation of detection methods for process-specific residual host cell protein and residual host cell DNA in biological preparation.

Tian, Yixiao; Wang, Xinyue; Shao, Dongyan et al. · 2024 · Cell biochemistry and function · DOI

Full title: Establishment and evaluation of detection methods for process-specific residual host cell protein and residual host cell DNA in biological preparation.

Authors: Tian, Yixiao; Wang, Xinyue; Shao, Dongyan; Zhao, Wen; Chen, Renan; Huang, Qingsheng

Year: 2024

Venue: Cell biochemistry and function

DOI: https://doi.org/10.1002/cbf.3986

Paper In silico mediated development of orthogonally selective mAb downstream processes for the removal of process-related impurities.

Jang, Dongyoun; Gutierrez-Diaz, Mario A; Altern, Scott H et al. · 2025 · Biotechnology progress · DOI

Full title: In silico mediated development of orthogonally selective mAb downstream processes for the removal of process-related impurities.

Authors: Jang, Dongyoun; Gutierrez-Diaz, Mario A; Altern, Scott H; Tjandra, Hendri; Cramer, Steven M

Year: 2025

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.70097

Paper Analytical characterization of host-cell-protein-rich aggregates in monoclonal antibody solutions.

Herman, Chase E; Min, Lie; Choe, Leila H et al. · 2023 · Biotechnology progress · DOI

Full title: Analytical characterization of host-cell-protein-rich aggregates in monoclonal antibody solutions.

Authors: Herman, Chase E; Min, Lie; Choe, Leila H; Maurer, Ronald W; Xu, Xuankuo; Ghose, Sanchayita; Lee, Kelvin H; Lenhoff, Abraham M

Year: 2023

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.3343

Paper A novel approach for protein identification from complex cell proteome using modified peptide mass fingerprinting algorithm.

Singh, Sumit Kumar; Goel, Garvit; Rathore, Anurag S · 2019 · Electrophoresis · DOI

Full title: A novel approach for protein identification from complex cell proteome using modified peptide mass fingerprinting algorithm.

Authors: Singh, Sumit Kumar; Goel, Garvit; Rathore, Anurag S

Year: 2019

Venue: Electrophoresis

DOI: https://doi.org/10.1002/elps.201900128

Paper Harnessing the power of electrophoresis and chromatography: Offline coupling of reverse phase liquid chromatography-capillary zone electrophoresis-tandem mass spectrometry for analysis of host cell proteins in monoclonal antibody producing CHO cell line.

Kumar, Ramesh; Shah, Rohan L; Ahmad, Shadab et al. · 2021 · Electrophoresis · DOI

Full title: Harnessing the power of electrophoresis and chromatography: Offline coupling of reverse phase liquid chromatography-capillary zone electrophoresis-tandem mass spectrometry for analysis of host cell proteins in monoclonal antibody producing CHO cell line.

Authors: Kumar, Ramesh; Shah, Rohan L; Ahmad, Shadab; Rathore, Anurag S

Year: 2021

Venue: Electrophoresis

DOI: https://doi.org/10.1002/elps.202000252

Paper Risk control of host cell proteins in one therapeutic antibody produced by concentrated fed-batch (CFB) mode.

Lu, Yiling; Lin, Jun; Bian, Tianze et al. · 2023 · Engineering in life sciences · DOI

Full title: Risk control of host cell proteins in one therapeutic antibody produced by concentrated fed-batch (CFB) mode.

Authors: Lu, Yiling; Lin, Jun; Bian, Tianze; Chen, Jin; Liu, Dan; Ma, Mingjun; Gao, Zhen; Chen, Jiemin; Ju, Dianwen; Wang, Xing

Year: 2023

Venue: Engineering in life sciences

DOI: https://doi.org/10.1002/elsc.202200060

Paper Comprehensive analysis of host cell impurities in monoclonal antibodies with improved sensitivity by capillary zone electrophoresis mass spectrometry.

Zhang, Zhenbin; Albanetti, Thomas; Linkous, Travis et al. · 2017 · Electrophoresis · DOI

Full title: Comprehensive analysis of host cell impurities in monoclonal antibodies with improved sensitivity by capillary zone electrophoresis mass spectrometry.

Authors: Zhang, Zhenbin; Albanetti, Thomas; Linkous, Travis; Larkin, Christopher J; Schoner, Ronald; McGivney, James B; Dovichi, Norman J

Year: 2017

Venue: Electrophoresis

DOI: https://doi.org/10.1002/elps.201600390

Paper Improving Downstream Process Related Manufacturability Based on Protein Engineering-A Feasibility Study.

Capito, Florian; Wong, Ting Hin; Faust, Christine et al. · 2024 · Engineering in life sciences · DOI

Full title: Improving Downstream Process Related Manufacturability Based on Protein Engineering-A Feasibility Study.

Authors: Capito, Florian; Wong, Ting Hin; Faust, Christine; Brand, Kilian; Dittrich, Werner; Sommerfeld, Mark; Tiwari, Garima; Langer, Thomas

Year: 2024

Venue: Engineering in life sciences

DOI: https://doi.org/10.1002/elsc.202400019

Paper Protein Equalizer™ Technology : The quest for a “democratic proteome”

Righetti, Pier Giorgio; Boschetti, Egisto; Lomas, Lee et al. · 2006 · PROTEOMICS · DOI

Full title: Protein Equalizer™ Technology : The quest for a “democratic proteome”

Authors: Righetti, Pier Giorgio; Boschetti, Egisto; Lomas, Lee; Citterio, Attilio

Year: 2006

Venue: PROTEOMICS

DOI: https://doi.org/10.1002/pmic.200500904

Paper Absolute quantitation of host cell proteins in recombinant human monoclonal antibodies with an automated CZE-ESI-MS/MS system.

Zhu, Guijie; Sun, Liangliang; Linkous, Travis et al. · 2014 · Electrophoresis · DOI

Full title: Absolute quantitation of host cell proteins in recombinant human monoclonal antibodies with an automated CZE-ESI-MS/MS system.

Authors: Zhu, Guijie; Sun, Liangliang; Linkous, Travis; Kernaghan, Dawn; McGivney, James B; Dovichi, Norman J

Year: 2014

Venue: Electrophoresis

DOI: https://doi.org/10.1002/elps.201300545

Paper Optimizing selectivity of anion hydrophobic multimodal chromatography for purification of a single-chain variable fragment.

Sakhnini, Laila I; Pedersen, Anja K; León, Ileana Rodríguez et al. · 2019 · Engineering in life sciences · DOI

Full title: Optimizing selectivity of anion hydrophobic multimodal chromatography for purification of a single-chain variable fragment.

Authors: Sakhnini, Laila I; Pedersen, Anja K; León, Ileana Rodríguez; Greisen, Per J; Hansen, Jens Jacob; Vester-Christensen, Malene B; Bülow, Leif; Dainiak, Maria B

Year: 2019

Venue: Engineering in life sciences

DOI: https://doi.org/10.1002/elsc.201800207

Paper Aqueous Two-Phase-Assisted Precipitation of Proteins: A Platform for Isolation of Process-Related Impurities from Therapeutic Proteins.

Rathore, Anurag S; Bhambure, R · 2021 · Methods in molecular biology (Clifton, N.J.) · DOI

Full title: Aqueous Two-Phase-Assisted Precipitation of Proteins: A Platform for Isolation of Process-Related Impurities from Therapeutic Proteins.

Authors: Rathore, Anurag S; Bhambure, R

Year: 2021

Venue: Methods in molecular biology (Clifton, N.J.)

DOI: https://doi.org/10.1007/978-1-0716-0775-6_8

Paper Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests.

Lavoie, R Ashton; Williams, Taufika Islam; Blackburn, R Kevin et al. · 2021 · Methods in molecular biology (Clifton, N.J.) · DOI

Full title: Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests.

Authors: Lavoie, R Ashton; Williams, Taufika Islam; Blackburn, R Kevin; Carbonell, Ruben G; Menegatti, Stefano

Year: 2021

Venue: Methods in molecular biology (Clifton, N.J.)

DOI: https://doi.org/10.1007/978-1-0716-1186-9_31

Paper Advanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: Benefits of FAIMS separation and gas-phase fractionation DIA.

Beaumal, Corentin; Beck, Alain; Hernandez-Alba, Oscar et al. · 2023 · Proteomics · DOI

Full title: Advanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: Benefits of FAIMS separation and gas-phase fractionation DIA.

Authors: Beaumal, Corentin; Beck, Alain; Hernandez-Alba, Oscar; Carapito, Christine

Year: 2023

Venue: Proteomics

DOI: https://doi.org/10.1002/pmic.202300172

Paper Improved detection of host cell proteins (HCPs) in a mammalian cell-derived antibody drug using liquid chromatography/mass spectrometry in conjunction with an HCP-enrichment strategy.

Thompson, Jenny Heidbrink; Chung, Wai Keen; Zhu, Min et al. · 2014 · Rapid communications in mass spectrometry : RCM · DOI

Full title: Improved detection of host cell proteins (HCPs) in a mammalian cell-derived antibody drug using liquid chromatography/mass spectrometry in conjunction with an HCP-enrichment strategy.

Authors: Thompson, Jenny Heidbrink; Chung, Wai Keen; Zhu, Min; Tie, Liu; Lu, Yali; Aboulaich, Nabila; Strouse, Robert; Mo, Wenjun David

Year: 2014

Venue: Rapid communications in mass spectrometry : RCM

DOI: https://doi.org/10.1002/rcm.6854

Paper Proteomic studies support the use of multi‐product immunoassays to monitor host cell protein impurities

Krawitz, Denise C.; Forrest, William F.; Moreno, G. Tony et al. · 2005 · PROTEOMICS · DOI

Full title: Proteomic studies support the use of multi‐product immunoassays to monitor host cell protein impurities

Authors: Krawitz, Denise C.; Forrest, William F.; Moreno, G. Tony; Kittleson, Joshua T.; Champion, Kathleen M.

Year: 2005

Venue: PROTEOMICS

DOI: https://doi.org/10.1002/pmic.200500225

Paper An innovative standard for LC-MS-based HCP profiling and accurate quantity assessment: Application to batch consistency in viral vaccine samples.

Trauchessec, Mathieu; Hesse, Anne Marie; Kraut, Alexandra et al. · 2021 · Proteomics · DOI

Full title: An innovative standard for LC-MS-based HCP profiling and accurate quantity assessment: Application to batch consistency in viral vaccine samples.

Authors: Trauchessec, Mathieu; Hesse, Anne Marie; Kraut, Alexandra; Berard, Yves; Herment, Laura; Fortin, Tanguy; Bruley, Christophe; Ferro, Myriam; Manin, Catherine

Year: 2021

Venue: Proteomics

DOI: https://doi.org/10.1002/pmic.202000152

Paper Removal of Residual DNA and Host Cell Proteins for the Purification of Recombinant Staphylokinase Expressed in <i>Escherichia coli</i>

Li, Guang; Li, Jing; Zhenxing, Zou et al. · 2024 · Separation Science Plus · DOI

Full title: Removal of Residual DNA and Host Cell Proteins for the Purification of Recombinant Staphylokinase Expressed in <i>Escherichia coli</i>

Authors: Li, Guang; Li, Jing; Zhenxing, Zou; Liu, Bo; Li, Weiping; Yang, Ding; Xuri, Song; Xiaolan, Fang; Dao-qi, HU

Year: 2024

Venue: Separation Science Plus

DOI: https://doi.org/10.1002/sscp.202300214

Paper Sample Preparation of Secreted Mammalian Host Cell Proteins and Their Characterization by Two-Dimensional Electrophoresis and Western Blotting.

Posch, Anton; Kollmann, Franziska; Berkelman, Thomas et al. · 2021 · Methods in molecular biology (Clifton, N.J.) · DOI

Full title: Sample Preparation of Secreted Mammalian Host Cell Proteins and Their Characterization by Two-Dimensional Electrophoresis and Western Blotting.

Authors: Posch, Anton; Kollmann, Franziska; Berkelman, Thomas; Dreskin, Elizabeth

Year: 2021

Venue: Methods in molecular biology (Clifton, N.J.)

DOI: https://doi.org/10.1007/978-1-0716-1186-9_32

Paper Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses.

Hogwood, Catherine E M; Chiverton, Lesley M; Mark Smales, C · 2017 · Methods in molecular biology (Clifton, N.J.) · DOI

Full title: Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses.

Authors: Hogwood, Catherine E M; Chiverton, Lesley M; Mark Smales, C

Year: 2017

Venue: Methods in molecular biology (Clifton, N.J.)

DOI: https://doi.org/10.1007/978-1-4939-6972-2_16

Paper Purification of Therapeutic Antibodies by Protein A Affinity Chromatography.

Rathore, Anurag S; Narnaware, Sharad · 2022 · Methods in molecular biology (Clifton, N.J.) · DOI

Full title: Purification of Therapeutic Antibodies by Protein A Affinity Chromatography.

Authors: Rathore, Anurag S; Narnaware, Sharad

Year: 2022

Venue: Methods in molecular biology (Clifton, N.J.)

DOI: https://doi.org/10.1007/978-1-0716-1450-1_9

Paper Analytical Tools for HCP Detection, Identification, and Quantitation.

Kanwal, Faiza; Henry, Michael; Meleady, Paula · 2025 · Methods in molecular biology (Clifton, N.J.) · DOI

Full title: Analytical Tools for HCP Detection, Identification, and Quantitation.

Authors: Kanwal, Faiza; Henry, Michael; Meleady, Paula

Year: 2025

Venue: Methods in molecular biology (Clifton, N.J.)

DOI: https://doi.org/10.1007/978-1-0716-4104-0_15

Paper Detection of Residual Host Cell Proteins in Biotherapeutic Drugs by Concatenated 2D LC-MS/MS.

Yang, Feng; Li, Delia; Walker, Donald E · 2022 · Methods in molecular biology (Clifton, N.J.) · DOI

Full title: Detection of Residual Host Cell Proteins in Biotherapeutic Drugs by Concatenated 2D LC-MS/MS.

Authors: Yang, Feng; Li, Delia; Walker, Donald E

Year: 2022

Venue: Methods in molecular biology (Clifton, N.J.)

DOI: https://doi.org/10.1007/978-1-0716-1450-1_16

Paper Analysis of host-cell proteins in biotherapeutic proteins by LC/MS approaches.

Doneanu, Catalin E; Chen, Weibin · 2014 · Methods in molecular biology (Clifton, N.J.) · DOI

Full title: Analysis of host-cell proteins in biotherapeutic proteins by LC/MS approaches.

Authors: Doneanu, Catalin E; Chen, Weibin

Year: 2014

Venue: Methods in molecular biology (Clifton, N.J.)

DOI: https://doi.org/10.1007/978-1-62703-977-2_25

Paper 2-D Western blotting for evaluation of antibodies developed for detection of host cell protein.

Berkelman, Tom; Harbers, Adriana; Bandhakavi, Sricharan · 2015 · Methods in molecular biology (Clifton, N.J.) · DOI

Full title: 2-D Western blotting for evaluation of antibodies developed for detection of host cell protein.

Authors: Berkelman, Tom; Harbers, Adriana; Bandhakavi, Sricharan

Year: 2015

Venue: Methods in molecular biology (Clifton, N.J.)

DOI: https://doi.org/10.1007/978-1-4939-2550-6_28

Paper Studying host cell protein based on 2D nano-LC-MS/MS: comparison between the removal and the reservation of the main antibody components.

Cui, Xinling; Li, Lei; Li, Ming et al. · 2025 · Analytical and bioanalytical chemistry · DOI

Full title: Studying host cell protein based on 2D nano-LC-MS/MS: comparison between the removal and the reservation of the main antibody components.

Authors: Cui, Xinling; Li, Lei; Li, Ming; Ying, Wantao; Cheng, Jinlan; Lin, Ling; Mi, Wei; Hu, Zhishang

Year: 2025

Venue: Analytical and bioanalytical chemistry

DOI: https://doi.org/10.1007/s00216-025-05786-3

Paper Analysis of Host Cell Proteins in Monoclonal Antibody Therapeutics Through Size Exclusion Chromatography.

Zhao, Bo; Abdubek, Polat; Zhang, Sisi et al. · 2022 · Pharmaceutical research · DOI

Full title: Analysis of Host Cell Proteins in Monoclonal Antibody Therapeutics Through Size Exclusion Chromatography.

Authors: Zhao, Bo; Abdubek, Polat; Zhang, Sisi; Xiao, Hui; Li, Ning

Year: 2022

Venue: Pharmaceutical research

DOI: https://doi.org/10.1007/s11095-022-03381-0

Paper Host cell contaminant protein assay development for recombinant biopharmaceuticals.

Eaton, L C · 1995 · Journal of chromatography. A · DOI

Full title: Host cell contaminant protein assay development for recombinant biopharmaceuticals.

Authors: Eaton, L C

Year: 1995

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/0021-9673(94)01249-e

Paper Measuring Lipolytic Activity to Support Process Improvements to Manage Lipase-Mediated Polysorbate Degradation.

Jahn, Michael; Zerr, Andreas; Fedorowicz, Filip Maciej et al. · 2020 · Pharmaceutical research · DOI

Full title: Measuring Lipolytic Activity to Support Process Improvements to Manage Lipase-Mediated Polysorbate Degradation.

Authors: Jahn, Michael; Zerr, Andreas; Fedorowicz, Filip Maciej; Brigger, Finn; Koulov, Atanas; Mahler, Hanns-Christian

Year: 2020

Venue: Pharmaceutical research

DOI: https://doi.org/10.1007/s11095-020-02812-0

Paper Ultrasensitive method for profiling host cell proteins by coupling limited digestion to ProteoMiner technology.

Zhang, Sisi; Xiao, Hui; Li, Ning · 2022 · Analytical biochemistry · DOI

Full title: Ultrasensitive method for profiling host cell proteins by coupling limited digestion to ProteoMiner technology.

Authors: Zhang, Sisi; Xiao, Hui; Li, Ning

Year: 2022

Venue: Analytical biochemistry

DOI: https://doi.org/10.1016/j.ab.2022.114901

Paper Development of a multiplexing method for the quantification of "high-risk" host cell lipases in biotherapeutics by Luminex.

Osko, Jeremy D; Rivera, Shannon; Wang, Fengqiang et al. · 2024 · Analytica chimica acta · DOI

Full title: Development of a multiplexing method for the quantification of "high-risk" host cell lipases in biotherapeutics by Luminex.

Authors: Osko, Jeremy D; Rivera, Shannon; Wang, Fengqiang; Niedringhaus, Thomas

Year: 2024

Venue: Analytica chimica acta

DOI: https://doi.org/10.1016/j.aca.2024.343349

Paper Separation of product associating E. coli host cell proteins OppA and DppA from recombinant apolipoprotein A-I(Milano) in an industrial HIC unit operation.

Hunter, Alan K; Wang, Xing; Suda, Eric J et al. · 2009 · Biotechnology progress · DOI

Full title: Separation of product associating E. coli host cell proteins OppA and DppA from recombinant apolipoprotein A-I(Milano) in an industrial HIC unit operation.

Authors: Hunter, Alan K; Wang, Xing; Suda, Eric J; Herberg, John T; Shell, Robert E; Thomas, Kristin E; Dufield, Robert L; Gustafson, Mark E; Mozier, Ned M; Ho, Sa V

Year: 2009

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.106

Paper Purification of proteins from solutions containing residual host cell proteins via preparative crystallization.

Hekmat, Dariusch; Breitschwerdt, Peter; Weuster-Botz, Dirk · 2015 · Biotechnology letters · DOI

Full title: Purification of proteins from solutions containing residual host cell proteins via preparative crystallization.

Authors: Hekmat, Dariusch; Breitschwerdt, Peter; Weuster-Botz, Dirk

Year: 2015

Venue: Biotechnology letters

DOI: https://doi.org/10.1007/s10529-015-1866-5

Paper Universal protocol and standard-spiking strategy for profiling of host cell proteins in therapeutic growth hormone.

Liu, Feng; Ren, Weicheng; Liu, Hailong et al. · 2023 · Analytical biochemistry · DOI

Full title: Universal protocol and standard-spiking strategy for profiling of host cell proteins in therapeutic growth hormone.

Authors: Liu, Feng; Ren, Weicheng; Liu, Hailong; Liu, Hao; Wang, Shuyue; Zhang, Di; Sun, Dandan; Zhang, Feifei; Shao, Zhengkang; Feng, Jia; Yan, Menghan; Yang, Lan; Wang, Zhiwei; Zhang, Jinliang; Wang, Yingwu

Year: 2023

Venue: Analytical biochemistry

DOI: https://doi.org/10.1016/j.ab.2023.115136

Paper A mass spectrometry-based approach to host cell protein identification and its application in a comparability exercise.

Reisinger, Veronika; Toll, Hansjoerg; Mayer, Robert Ernst et al. · 2014 · Analytical biochemistry · DOI

Full title: A mass spectrometry-based approach to host cell protein identification and its application in a comparability exercise.

Authors: Reisinger, Veronika; Toll, Hansjoerg; Mayer, Robert Ernst; Visser, Jan; Wolschin, Florian

Year: 2014

Venue: Analytical biochemistry

DOI: https://doi.org/10.1016/j.ab.2014.06.005

Paper Improved host cell protein analysis in monoclonal antibody products through ProteoMiner.

Chen, I-Hsuan; Xiao, Hui; Li, Ning · 2020 · Analytical biochemistry · DOI

Full title: Improved host cell protein analysis in monoclonal antibody products through ProteoMiner.

Authors: Chen, I-Hsuan; Xiao, Hui; Li, Ning

Year: 2020

Venue: Analytical biochemistry

DOI: https://doi.org/10.1016/j.ab.2020.113972

Paper Comprehensive host cell proteins profiling in biopharmaceuticals by a sensitivity enhanced mass spectrometry strategy using TMT-labeling and signal boosting.

Xue, Andong; Kong, Linlin; Li, Jialin et al. · 2025 · Analytica chimica acta · DOI

Full title: Comprehensive host cell proteins profiling in biopharmaceuticals by a sensitivity enhanced mass spectrometry strategy using TMT-labeling and signal boosting.

Authors: Xue, Andong; Kong, Linlin; Li, Jialin; Jiao, Yuxin; Hu, Zhishang; Fu, Bin; Wang, Guibin; Zhang, Wanjun; Li, Jianheng; Qin, Weijie

Year: 2025

Venue: Analytica chimica acta

DOI: https://doi.org/10.1016/j.aca.2024.343445

Paper Host cell protein testing strategy for hepatitis B antigen in Hexavalent vaccine - Towards a general testing strategy for recombinant vaccines.

Toinon, Audrey; Fontaine, Christelle; Thion, Laurent et al. · 2018 · Biologicals : journal of the International Association of Biological Standardization · DOI

Full title: Host cell protein testing strategy for hepatitis B antigen in Hexavalent vaccine - Towards a general testing strategy for recombinant vaccines.

Authors: Toinon, Audrey; Fontaine, Christelle; Thion, Laurent; Gajewska, Beata; Carpick, Bruce; Nougarede, Nolwenn; Uhlrich, Sylvie

Year: 2018

Venue: Biologicals : journal of the International Association of Biological Standardization

DOI: https://doi.org/10.1016/j.biologicals.2018.05.006

Paper A highly sensitive and robust LC-MS platform for host cell protein characterization in biotherapeutics.

Ji, Qinqin; Sokolowska, Izabela; Cao, Rui et al. · 2023 · Biologicals : journal of the International Association of Biological Standardization · DOI

Full title: A highly sensitive and robust LC-MS platform for host cell protein characterization in biotherapeutics.

Authors: Ji, Qinqin; Sokolowska, Izabela; Cao, Rui; Jiang, Yulei; Mo, Jingjie; Hu, Ping

Year: 2023

Venue: Biologicals : journal of the International Association of Biological Standardization

DOI: https://doi.org/10.1016/j.biologicals.2023.101675

Paper Characterization of host cell proteins in the downstream process of plant-Based biologics using LC-MS profiling.

Panapitakkul, Chalisa; Bulaon, Christine Joy I; Pisuttinusart, Nuttapat et al. · 2024 · Biotechnology reports (Amsterdam, Netherlands) · DOI

Full title: Characterization of host cell proteins in the downstream process of plant-Based biologics using LC-MS profiling.

Authors: Panapitakkul, Chalisa; Bulaon, Christine Joy I; Pisuttinusart, Nuttapat; Phoolcharoen, Waranyoo

Year: 2024

Venue: Biotechnology reports (Amsterdam, Netherlands)

DOI: https://doi.org/10.1016/j.btre.2024.e00856

Paper Hydrophobic interaction chromatography as polishing step enables obtaining ultra-pure recombinant antibodies.

Pereira Bresolin, Iara Rocha Antunes; Lingg, Nico; Bresolin, Igor Tadeu Lazzarotto et al. · 2020 · Journal of biotechnology · DOI

Full title: Hydrophobic interaction chromatography as polishing step enables obtaining ultra-pure recombinant antibodies.

Authors: Pereira Bresolin, Iara Rocha Antunes; Lingg, Nico; Bresolin, Igor Tadeu Lazzarotto; Jungbauer, Alois

Year: 2020

Venue: Journal of biotechnology

DOI: https://doi.org/10.1016/j.btecx.2020.100020

Paper Single step plasmid DNA purification using methacrylate monolith bearing combination of ion-exchange and hydrophobic groups.

Smrekar, Vida; Smrekar, Franc; Strancar, Aleš et al. · 2013 · Journal of chromatography. A · DOI

Full title: Single step plasmid DNA purification using methacrylate monolith bearing combination of ion-exchange and hydrophobic groups.

Authors: Smrekar, Vida; Smrekar, Franc; Strancar, Aleš; Podgornik, Aleš

Year: 2013

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2012.12.029

Paper Proteomics-based, multivariate random forest method for prediction of protein separation behavior during cation-exchange chromatography.

Swanson, Ryan K; Xu, Ruo; Nettleton, Dan et al. · 2012 · Journal of chromatography. A · DOI

Full title: Proteomics-based, multivariate random forest method for prediction of protein separation behavior during cation-exchange chromatography.

Authors: Swanson, Ryan K; Xu, Ruo; Nettleton, Dan; Glatz, Charles E

Year: 2012

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2012.06.009

Paper Accelerated purification process development of monoclonal antibodies for shortening time to clinic. Design and case study of chromatography processes.

Ishihara, Takashi; Kadoya, Toshihiko · 2007 · Journal of chromatography. A · DOI

Full title: Accelerated purification process development of monoclonal antibodies for shortening time to clinic. Design and case study of chromatography processes.

Authors: Ishihara, Takashi; Kadoya, Toshihiko

Year: 2007

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2007.10.104

Paper Antibody capture by mixed-mode chromatography: a comprehensive study from determination of optimal purification conditions to identification of contaminating host cell proteins.

Pezzini, Jerome; Joucla, Gilles; Gantier, René et al. · 2011 · Journal of chromatography. A · DOI

Full title: Antibody capture by mixed-mode chromatography: a comprehensive study from determination of optimal purification conditions to identification of contaminating host cell proteins.

Authors: Pezzini, Jerome; Joucla, Gilles; Gantier, René; Toueille, Magali; Lomenech, Anne-Marie; Le Sénéchal, Caroline; Garbay, Bertrand; Santarelli, Xavier; Cabanne, Charlotte

Year: 2011

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2011.09.036

Paper Purification process of recombinant monoclonal antibodies with mixed mode chromatography.

Maria, Sophie; Joucla, Gilles; Garbay, Bertrand et al. · 2015 · Journal of chromatography. A · DOI

Full title: Purification process of recombinant monoclonal antibodies with mixed mode chromatography.

Authors: Maria, Sophie; Joucla, Gilles; Garbay, Bertrand; Dieryck, Wilfrid; Lomenech, Anne-Marie; Santarelli, Xavier; Cabanne, Charlotte

Year: 2015

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2015.03.018

Paper Characterization of membrane adsorbers used for impurity removal during the continuous purification of monoclonal antibodies.

Trnovec, Helena; Doles, Tibor; Hribar, Gorazd et al. · 2020 · Journal of chromatography. A · DOI

Full title: Characterization of membrane adsorbers used for impurity removal during the continuous purification of monoclonal antibodies.

Authors: Trnovec, Helena; Doles, Tibor; Hribar, Gorazd; Furlan, Nebojša; Podgornik, Aleš

Year: 2020

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2019.460518

Paper A two-step heat treatment of cell disruption supernatant enables efficient removal of host cell proteins before chromatographic purification of HBc particles.

Li, Zhengjun; Wei, Jiangxue; Yang, Yanli et al. · 2018 · Journal of chromatography. A · DOI

Full title: A two-step heat treatment of cell disruption supernatant enables efficient removal of host cell proteins before chromatographic purification of HBc particles.

Authors: Li, Zhengjun; Wei, Jiangxue; Yang, Yanli; Liu, Lili; Ma, Guanghui; Zhang, Songping; Su, Zhiguo

Year: 2018

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2018.10.050

Paper Residual on column host cell protein analysis during lifetime studies of protein A chromatography.

Lintern, Katherine; Pathak, Mili; Smales, C Mark et al. · 2016 · Journal of chromatography. A · DOI

Full title: Residual on column host cell protein analysis during lifetime studies of protein A chromatography.

Authors: Lintern, Katherine; Pathak, Mili; Smales, C Mark; Howland, Kevin; Rathore, Anurag; Bracewell, Daniel G

Year: 2016

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2016.07.055

Paper Evaluation of chromatofocusing as a capture method for monoclonal antibody products.

Liu, Yang; Deldari, Sevda; Guo, Hui et al. · 2018 · Journal of chromatography. A · DOI

Full title: Evaluation of chromatofocusing as a capture method for monoclonal antibody products.

Authors: Liu, Yang; Deldari, Sevda; Guo, Hui; Narahari, Chittoor R; Bates, Ronald C; Swanson, Ryan; Ghose, Sanchayita; Li, Zheng Jian; Frey, Douglas D

Year: 2018

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2018.07.028

Paper Continuous precipitation of process related impurities from clarified cell culture supernatant using a novel coiled flow inversion reactor (CFIR).

Kateja, Nikhil; Agarwal, Harshit; Saraswat, Aditya et al. · 2016 · Biotechnology journal · DOI

Full title: Continuous precipitation of process related impurities from clarified cell culture supernatant using a novel coiled flow inversion reactor (CFIR).

Authors: Kateja, Nikhil; Agarwal, Harshit; Saraswat, Aditya; Bhat, Manish; Rathore, Anurag S

Year: 2016

Venue: Biotechnology journal

DOI: https://doi.org/10.1002/biot.201600271

Paper Studying host cell protein interactions with monoclonal antibodies using high throughput protein A chromatography.

Sisodiya, Vikram N; Lequieu, Joshua; Rodriguez, Maricel et al. · 2012 · Biotechnology journal · DOI

Full title: Studying host cell protein interactions with monoclonal antibodies using high throughput protein A chromatography.

Authors: Sisodiya, Vikram N; Lequieu, Joshua; Rodriguez, Maricel; McDonald, Paul; Lazzareschi, Kathlyn P

Year: 2012

Venue: Biotechnology journal

DOI: https://doi.org/10.1002/biot.201100479

Paper Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins.

Arfi, Zulfaquar Ahmad; Hellwig, Stephan; Drossard, Jürgen et al. · 2016 · Biotechnology journal · DOI

Full title: Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisCO depletion and the removal of endotoxins.

Authors: Arfi, Zulfaquar Ahmad; Hellwig, Stephan; Drossard, Jürgen; Fischer, Rainer; Buyel, Johannes Felix

Year: 2016

Venue: Biotechnology journal

DOI: https://doi.org/10.1002/biot.201500271

Paper Characterization of Residual Host Cell Protein Impurities in Biotherapeutics

Krawitz, Denise C.; Rouse, Jason C.; Sperry, Justin B. et al. · 2017 · DOI

Full title: Characterization of Residual Host Cell Protein Impurities in Biotherapeutics

Authors: Krawitz, Denise C.; Rouse, Jason C.; Sperry, Justin B.; Sandoval, Wendy; Vanderlaan, Martin

Year: 2017

DOI: https://doi.org/10.1002/9781119384434.ch10

Paper Host cell protein analysis in therapeutic protein bioprocessing - methods and applications.

Tscheliessnig, Anne Luise; Konrath, Julita; Bates, Ron et al. · 2013 · Biotechnology journal · DOI

Full title: Host cell protein analysis in therapeutic protein bioprocessing - methods and applications.

Authors: Tscheliessnig, Anne Luise; Konrath, Julita; Bates, Ron; Jungbauer, Alois

Year: 2013

Venue: Biotechnology journal

DOI: https://doi.org/10.1002/biot.201200018

Paper Bioinformatic analysis of Chinese hamster ovary host cell protein lipases.

MacDonald, Madolyn L; Hamaker, Nathaniel; Lee, Kelvin H · 2018 · AIChE journal. American Institute of Chemical Engineers · DOI

Full title: Bioinformatic analysis of Chinese hamster ovary host cell protein lipases.

Authors: MacDonald, Madolyn L; Hamaker, Nathaniel; Lee, Kelvin H

Year: 2018

Venue: AIChE journal. American Institute of Chemical Engineers

DOI: https://doi.org/10.1002/aic.16378

Paper Generic chromatography-based purification strategies accelerate the development of downstream processes for biopharmaceutical proteins produced in plants.

Buyel, Johannes F; Fischer, Rainer · 2014 · Biotechnology journal · DOI

Full title: Generic chromatography-based purification strategies accelerate the development of downstream processes for biopharmaceutical proteins produced in plants.

Authors: Buyel, Johannes F; Fischer, Rainer

Year: 2014

Venue: Biotechnology journal

DOI: https://doi.org/10.1002/biot.201300548

Paper A combined pH and temperature precipitation step facilitates the purification of tobacco-derived recombinant proteins that are sensitive to extremes of either parameter.

Opdensteinen, Patrick; Lobanov, Aleksandr; Buyel, Johannes Felix · 2021 · Biotechnology journal · DOI

Full title: A combined pH and temperature precipitation step facilitates the purification of tobacco-derived recombinant proteins that are sensitive to extremes of either parameter.

Authors: Opdensteinen, Patrick; Lobanov, Aleksandr; Buyel, Johannes Felix

Year: 2021

Venue: Biotechnology journal

DOI: https://doi.org/10.1002/biot.202000340

Paper Quantitative definition and monitoring of the host cell protein proteome using iTRAQ - a study of an industrial mAb producing CHO-S cell line.

Chiverton, Lesley M; Evans, Caroline; Pandhal, Jagroop et al. · 2016 · Biotechnology journal · DOI

Full title: Quantitative definition and monitoring of the host cell protein proteome using iTRAQ - a study of an industrial mAb producing CHO-S cell line.

Authors: Chiverton, Lesley M; Evans, Caroline; Pandhal, Jagroop; Landels, Andrew R; Rees, Byron J; Levison, Peter R; Wright, Phillip C; Smales, C Mark

Year: 2016

Venue: Biotechnology journal

DOI: https://doi.org/10.1002/biot.201500550

Paper An ultra scale-down approach identifies host cell protein differences across a panel of mAb producing CHO cell line variants.

Hogwood, Catherine E M; Ahmad, Shahina S; Tarrant, Richard D et al. · 2016 · Biotechnology journal · DOI

Full title: An ultra scale-down approach identifies host cell protein differences across a panel of mAb producing CHO cell line variants.

Authors: Hogwood, Catherine E M; Ahmad, Shahina S; Tarrant, Richard D; Bracewell, Daniel G; Smales, C Mark

Year: 2016

Venue: Biotechnology journal

DOI: https://doi.org/10.1002/biot.201500010

Paper Toward improved host cell protein impurity assessment

Baik, Jong Youn; Lee, Kelvin H. · 2016 · Biotechnology Journal · DOI

Full title: Toward improved host cell protein impurity assessment

Authors: Baik, Jong Youn; Lee, Kelvin H.

Year: 2016

Venue: Biotechnology Journal

DOI: https://doi.org/10.1002/biot.201600223

Paper Continuous purification of antibodies from cell culture supernatant with aqueous two‐phase systems: From concept to process

Rosa, Paula A.J.; Azevedo, Ana M.; Sommerfeld, Sven D. et al. · 2012 · Biotechnology Journal · DOI

Full title: Continuous purification of antibodies from cell culture supernatant with aqueous two‐phase systems: From concept to process

Authors: Rosa, Paula A.J.; Azevedo, Ana M.; Sommerfeld, Sven D.; Mutter, M.; Bäcker, W.; Aires‐Barros, M. Raquel

Year: 2012

Venue: Biotechnology Journal

DOI: https://doi.org/10.1002/biot.201200031

Paper High‐throughput screening of packed‐bed chromatography coupled with SELDI‐TOF MS analysis: monoclonal antibodies versus host cell protein

Wierling, P. Schulze; Bogumil, Ralf; Knieps‐Grünhagen, Esther et al. · 2007 · Biotechnology and Bioengineering · DOI

Full title: High‐throughput screening of packed‐bed chromatography coupled with SELDI‐TOF MS analysis: monoclonal antibodies versus host cell protein

Authors: Wierling, P. Schulze; Bogumil, Ralf; Knieps‐Grünhagen, Esther; Hubbuch, Jürgen

Year: 2007

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.21399

Paper Host cell proteins in biologics development: Identification, quantitation and risk assessment.

Wang, Xing; Hunter, Alan K; Mozier, Ned M · 2009 · Biotechnology and bioengineering · DOI

Full title: Host cell proteins in biologics development: Identification, quantitation and risk assessment.

Authors: Wang, Xing; Hunter, Alan K; Mozier, Ned M

Year: 2009

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.22304

Paper Application of high-performance tangential flow filtration (HPTFF) to the purification of a human pharmaceutical antibody fragment expressed in Escherichia coli.

Lebreton, Bénédicte; Brown, Arick; van Reis, Robert · 2008 · Biotechnology and bioengineering · DOI

Full title: Application of high-performance tangential flow filtration (HPTFF) to the purification of a human pharmaceutical antibody fragment expressed in Escherichia coli.

Authors: Lebreton, Bénédicte; Brown, Arick; van Reis, Robert

Year: 2008

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.21842

Paper Integrated Chromatographic Platform for Simultaneous Separation of Charge Variants and Aggregates from Monoclonal Antibody Therapeutic Products.

Kateja, Nikhil; Kumar, Devashish; Godara, Avinash et al. · 2017 · Biotechnology journal · DOI

Full title: Integrated Chromatographic Platform for Simultaneous Separation of Charge Variants and Aggregates from Monoclonal Antibody Therapeutic Products.

Authors: Kateja, Nikhil; Kumar, Devashish; Godara, Avinash; Kumar, Vijesh; Rathore, Anurag S

Year: 2017

Venue: Biotechnology journal

DOI: https://doi.org/10.1002/biot.201700133

Paper Integrated solution to purification challenges in the manufacture of a soluble recombinant protein in E. coli.

Caparon, Maire H; Rust, Kevin J; Hunter, Alan K et al. · 2010 · Biotechnology and bioengineering · DOI

Full title: Integrated solution to purification challenges in the manufacture of a soluble recombinant protein in E. coli.

Authors: Caparon, Maire H; Rust, Kevin J; Hunter, Alan K; McLaughlin, Joseph K; Thomas, Kristen E; Herberg, John T; Shell, Robert E; Lanter, Paul B; Bishop, Bruce F; Dufield, Robert L; Wang, Xing; Ho, Sa V

Year: 2010

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.22542

Paper Recovery of Chinese hamster ovary host cell proteins for proteomic analysis.

Valente, Kristin N; Schaefer, Amy K; Kempton, Hannah R et al. · 2014 · Biotechnology journal · DOI

Full title: Recovery of Chinese hamster ovary host cell proteins for proteomic analysis.

Authors: Valente, Kristin N; Schaefer, Amy K; Kempton, Hannah R; Lenhoff, Abraham M; Lee, Kelvin H

Year: 2014

Venue: Biotechnology journal

DOI: https://doi.org/10.1002/biot.201300190

Paper The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody purification process.

Hogwood, Catherine E M; Tait, Andrew S; Koloteva-Levine, Nadejda et al. · 2013 · Biotechnology and bioengineering · DOI

Full title: The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody purification process.

Authors: Hogwood, Catherine E M; Tait, Andrew S; Koloteva-Levine, Nadejda; Bracewell, Daniel G; Smales, C Mark

Year: 2013

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.24607

Paper Proteomics Reveals Distinctive Host Cell Protein Expression Patterns in Fed-Batch and Perfusion Cell Culture Processes.

Sahoo, Ansuman; Tsukiadate, Taku; Lin, Bor-Ruei et al. · 2025 · Biotechnology journal · DOI

Full title: Proteomics Reveals Distinctive Host Cell Protein Expression Patterns in Fed-Batch and Perfusion Cell Culture Processes.

Authors: Sahoo, Ansuman; Tsukiadate, Taku; Lin, Bor-Ruei; Kotzbauer, Erin; Houser, Jason; Patel, Misaal; Li, Xuanwen; Madabhushi, Sri Ranganayaki

Year: 2025

Venue: Biotechnology journal

DOI: https://doi.org/10.1002/biot.202400567

Paper Anion exchange membrane adsorbers for flow‐through polishing steps: Part II. Virus, host cell protein, DNA clearance, and antibody recovery

Weaver, Justin; Husson, Scott M.; Murphy, Louise et al. · 2012 · Biotechnology and Bioengineering · DOI

Full title: Anion exchange membrane adsorbers for flow‐through polishing steps: Part II. Virus, host cell protein, DNA clearance, and antibody recovery

Authors: Weaver, Justin; Husson, Scott M.; Murphy, Louise; Wickramasinghe, S. Ranil

Year: 2012

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.24724

Paper Profiling of host cell proteins by two-dimensional difference gel electrophoresis (2D-DIGE): Implications for downstream process development.

Jin, Mi; Szapiel, Nicolas; Zhang, Jennifer et al. · 2010 · Biotechnology and bioengineering · DOI

Full title: Profiling of host cell proteins by two-dimensional difference gel electrophoresis (2D-DIGE): Implications for downstream process development.

Authors: Jin, Mi; Szapiel, Nicolas; Zhang, Jennifer; Hickey, John; Ghose, Sanchayita

Year: 2010

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.22532

Paper Evaluation of high-capacity cation exchange chromatography for direct capture of monoclonal antibodies from high-titer cell culture processes.

Tao, Yinying; Ibraheem, Aladein; Conley, Lynn et al. · 2014 · Biotechnology and bioengineering · DOI

Full title: Evaluation of high-capacity cation exchange chromatography for direct capture of monoclonal antibodies from high-titer cell culture processes.

Authors: Tao, Yinying; Ibraheem, Aladein; Conley, Lynn; Cecchini, Douglas; Ghose, Sanchayita

Year: 2014

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.25192

Paper Identification and characterization of host cell protein product‐associated impurities in monoclonal antibody bioprocessing

Levy, Nicholas E.; Valente, Kristin N.; Choe, Leila H. et al. · 2013 · Biotechnology and Bioengineering · DOI

Full title: Identification and characterization of host cell protein product‐associated impurities in monoclonal antibody bioprocessing

Authors: Levy, Nicholas E.; Valente, Kristin N.; Choe, Leila H.; Lee, Kelvin H.; Lenhoff, Abraham M.

Year: 2013

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.25158

Paper CHOPPI: a web tool for the analysis of immunogenicity risk from host cell proteins in CHO-based protein production.

Bailey-Kellogg, Chris; Gutiérrez, Andres H; Moise, Leonard et al. · 2014 · Biotechnology and bioengineering · DOI

Full title: CHOPPI: a web tool for the analysis of immunogenicity risk from host cell proteins in CHO-based protein production.

Authors: Bailey-Kellogg, Chris; Gutiérrez, Andres H; Moise, Leonard; Terry, Frances; Martin, William D; De Groot, Anne S

Year: 2014

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.25286

Paper Degradation of an Fc‐fusion recombinant protein by host cell proteases: Identification of a CHO cathepsin D protease

Robert, Flavie; Bierau, Horst; Rossi, Mara et al. · 2009 · Biotechnology and Bioengineering · DOI

Full title: Degradation of an Fc‐fusion recombinant protein by host cell proteases: Identification of a CHO cathepsin D protease

Authors: Robert, Flavie; Bierau, Horst; Rossi, Mara; Agugiaro, David; Soranzo, Thomas; Broly, Hervé; Mitchell-Logean, Christine M.

Year: 2009

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.22494

Paper Host cell protein dynamics in the supernatant of a mAb producing CHO cell line.

Tait, A S; Hogwood, C E M; Smales, C M et al. · 2012 · Biotechnology and bioengineering · DOI

Full title: Host cell protein dynamics in the supernatant of a mAb producing CHO cell line.

Authors: Tait, A S; Hogwood, C E M; Smales, C M; Bracewell, D G

Year: 2012

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.24383

Paper Host cell protein testing by ELISAs and the use of orthogonal methods.

Zhu-Shimoni, Judith; Yu, Christopher; Nishihara, Julie et al. · 2014 · Biotechnology and bioengineering · DOI

Full title: Host cell protein testing by ELISAs and the use of orthogonal methods.

Authors: Zhu-Shimoni, Judith; Yu, Christopher; Nishihara, Julie; Wong, Robert M; Gunawan, Feny; Lin, Margaret; Krawitz, Denise; Liu, Peter; Sandoval, Wendy; Vanderlaan, Martin

Year: 2014

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.25327

Paper Immunoglobulin G elution in protein A chromatography employing the method of chromatofocusing for reducing the co-elution of impurities.

Pinto, Nuno D S; Uplekar, Shaunak D; Moreira, Antonio R et al. · 2017 · Biotechnology and bioengineering · DOI

Full title: Immunoglobulin G elution in protein A chromatography employing the method of chromatofocusing for reducing the co-elution of impurities.

Authors: Pinto, Nuno D S; Uplekar, Shaunak D; Moreira, Antonio R; Rao, Govind; Frey, Douglas D

Year: 2017

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.26053

Paper Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification

Levy, Nicholas E.; Valente, Kristin N.; Lee, Kelvin H. et al. · 2015 · Biotechnology and Bioengineering · DOI

Full title: Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification

Authors: Levy, Nicholas E.; Valente, Kristin N.; Lee, Kelvin H.; Lenhoff, Abraham M.

Year: 2015

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.25882

Paper Host cell protein quantification by Fourier transform mid infrared spectroscopy (FT-MIR).

Capito, Florian; Skudas, Romas; Kolmar, Harald et al. · 2013 · Biotechnology and bioengineering · DOI

Full title: Host cell protein quantification by Fourier transform mid infrared spectroscopy (FT-MIR).

Authors: Capito, Florian; Skudas, Romas; Kolmar, Harald; Stanislawski, Bernd

Year: 2013

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.24611

Paper More similar than different: Host cell protein production using three null CHO cell lines.

Yuk, Inn H; Nishihara, Julie; Walker, Donald et al. · 2015 · Biotechnology and bioengineering · DOI

Full title: More similar than different: Host cell protein production using three null CHO cell lines.

Authors: Yuk, Inn H; Nishihara, Julie; Walker, Donald; Huang, Eric; Gunawan, Feny; Subramanian, Jayashree; Pynn, Abigail F J; Yu, X Christopher; Zhu-Shimoni, Judith; Vanderlaan, Martin; Krawitz, Denise C

Year: 2015

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.25615

Paper Development of a novel and efficient cell culture flocculation process using a stimulus responsive polymer to streamline antibody purification processes.

Kang, Yun Kenneth; Hamzik, James; Felo, Michael et al. · 2013 · Biotechnology and bioengineering · DOI

Full title: Development of a novel and efficient cell culture flocculation process using a stimulus responsive polymer to streamline antibody purification processes.

Authors: Kang, Yun Kenneth; Hamzik, James; Felo, Michael; Qi, Bo; Lee, Julia; Ng, Stanley; Liebisch, Gregory; Shanehsaz, Behnam; Singh, Nripen; Persaud, Kris; Ludwig, Dale L; Balderes, Paul

Year: 2013

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.24969

Paper Expression of difficult-to-remove host cell protein impurities during extended Chinese hamster ovary cell culture and their impact on continuous bioprocessing.

Valente, Kristin N; Lenhoff, Abraham M; Lee, Kelvin H · 2015 · Biotechnology and bioengineering · DOI

Full title: Expression of difficult-to-remove host cell protein impurities during extended Chinese hamster ovary cell culture and their impact on continuous bioprocessing.

Authors: Valente, Kristin N; Lenhoff, Abraham M; Lee, Kelvin H

Year: 2015

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.25515

Paper Comparison of purification strategies for antibodies used in a broad spectrum host cell protein immunoassay.

Baldus, Phoebe A; Brown, Matthew; Wright, Richard S et al. · 2018 · Biotechnology and bioengineering · DOI

Full title: Comparison of purification strategies for antibodies used in a broad spectrum host cell protein immunoassay.

Authors: Baldus, Phoebe A; Brown, Matthew; Wright, Richard S; Campbell, Jessica A; Lute, Scott; Chavez, Brittany; Brorson, Kurt; Mozier, Ned

Year: 2018

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.26482

Paper Cascading effect in bioprocessing-The impact of mild hypothermia on CHO cell behavior and host cell protein composition.

Goey, Cher H; Tsang, Joshua M H; Bell, David et al. · 2017 · Biotechnology and bioengineering · DOI

Full title: Cascading effect in bioprocessing-The impact of mild hypothermia on CHO cell behavior and host cell protein composition.

Authors: Goey, Cher H; Tsang, Joshua M H; Bell, David; Kontoravdi, Cleo

Year: 2017

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.26437

Paper The future of host cell protein (HCP) identification during process development and manufacturing linked to a risk-based management for their control.

Bracewell, Daniel G; Francis, Richard; Smales, C Mark · 2015 · Biotechnology and bioengineering · DOI

Full title: The future of host cell protein (HCP) identification during process development and manufacturing linked to a risk-based management for their control.

Authors: Bracewell, Daniel G; Francis, Richard; Smales, C Mark

Year: 2015

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.25628

Paper Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations.

Chiu, Josephine; Valente, Kristin N; Levy, Nicholas E et al. · 2017 · Biotechnology and bioengineering · DOI

Full title: Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations.

Authors: Chiu, Josephine; Valente, Kristin N; Levy, Nicholas E; Min, Lie; Lenhoff, Abraham M; Lee, Kelvin H

Year: 2017

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.26237

Paper A novel approach to characterize host cell proteins associated with therapeutic monoclonal antibodies.

Thomson, Andrew S; Mai, Shing; Byrne, Michael P · 2017 · Biotechnology and bioengineering · DOI

Full title: A novel approach to characterize host cell proteins associated with therapeutic monoclonal antibodies.

Authors: Thomson, Andrew S; Mai, Shing; Byrne, Michael P

Year: 2017

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.26256

Paper Development of robust antibody purification by optimizing protein-A chromatography in combination with precipitation methodologies.

Chollangi, Srinivas; Parker, Ray; Singh, Nripen et al. · 2015 · Biotechnology and bioengineering · DOI

Full title: Development of robust antibody purification by optimizing protein-A chromatography in combination with precipitation methodologies.

Authors: Chollangi, Srinivas; Parker, Ray; Singh, Nripen; Li, Yi; Borys, Michael; Li, Zhengjian

Year: 2015

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.25639

Paper Targeted capture of Chinese hamster ovary host cell proteins: Peptide ligand binding by proteomic analysis.

Lavoie, R Ashton; di Fazio, Alice; Williams, Taufika Islam et al. · 2020 · Biotechnology and bioengineering · DOI

Full title: Targeted capture of Chinese hamster ovary host cell proteins: Peptide ligand binding by proteomic analysis.

Authors: Lavoie, R Ashton; di Fazio, Alice; Williams, Taufika Islam; Carbonell, Ruben; Menegatti, Stefano

Year: 2020

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.27213

Paper The agony of choice: Impact of the host animal species on the enzyme-linked immunosorbent assay performance for host cell protein quantification.

Seisenberger, Christina; Graf, Tobias; Sticht, Sarah et al. · 2023 · Biotechnology and bioengineering · DOI

Full title: The agony of choice: Impact of the host animal species on the enzyme-linked immunosorbent assay performance for host cell protein quantification.

Authors: Seisenberger, Christina; Graf, Tobias; Sticht, Sarah; Haindl, Markus; Mohn, Ulrich; Wegele, Harald; Wiedmann, Michael; Wohlrab, Stefanie

Year: 2023

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28265

Paper High-performance countercurrent membrane purification for host cell protein removal from monoclonal antibody products.

Mohammadzadehmarandi, Aylin; Determan, Amy; Krumm, Christian et al. · 2023 · Biotechnology and bioengineering · DOI

Full title: High-performance countercurrent membrane purification for host cell protein removal from monoclonal antibody products.

Authors: Mohammadzadehmarandi, Aylin; Determan, Amy; Krumm, Christian; McIntosh, Lucas D; Zydney, Andrew L

Year: 2023

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28531

Paper Targeted CHO cell engineering approaches can reduce HCP-related enzymatic degradation and improve mAb product quality.

Dovgan, Tatiana; Golghalyani, Vahid; Zurlo, Fabio et al. · 2021 · Biotechnology and bioengineering · DOI

Full title: Targeted CHO cell engineering approaches can reduce HCP-related enzymatic degradation and improve mAb product quality.

Authors: Dovgan, Tatiana; Golghalyani, Vahid; Zurlo, Fabio; Hatton, Diane; Lindo, Viv; Turner, Richard; Harris, Claire; Cui, Tingting

Year: 2021

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.27857

Paper Comparison of platform host cell protein ELISA to process-specific host cell protein ELISA.

Gunawan, Feny; Nishihara, Julie; Liu, Peter et al. · 2018 · Biotechnology and bioengineering · DOI

Full title: Comparison of platform host cell protein ELISA to process-specific host cell protein ELISA.

Authors: Gunawan, Feny; Nishihara, Julie; Liu, Peter; Sandoval, Wendy; Vanderlaan, Marty; Zhang, Heidi; Krawitz, Denise

Year: 2018

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.26466

Paper Best practices on critical reagent characterization, qualification, and life cycle management for HCP immunoassays.

Graf, Tobias; Seisenberger, Christina; Wiedmann, Michael et al. · 2021 · Biotechnology and bioengineering · DOI

Full title: Best practices on critical reagent characterization, qualification, and life cycle management for HCP immunoassays.

Authors: Graf, Tobias; Seisenberger, Christina; Wiedmann, Michael; Wohlrab, Stefanie; Anderka, Oliver

Year: 2021

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.27881

Paper Impact of freeze-thaw processes on monoclonal antibody platform process development.

Weber, Dennis; Sittig, Christian; Hubbuch, Jürgen · 2021 · Biotechnology and bioengineering · DOI

Full title: Impact of freeze-thaw processes on monoclonal antibody platform process development.

Authors: Weber, Dennis; Sittig, Christian; Hubbuch, Jürgen

Year: 2021

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.27867

Paper Questioning coverage values determined by 2D western blots: A critical study on the characterization of anti-HCP ELISA reagents.

Seisenberger, Christina; Graf, Tobias; Haindl, Markus et al. · 2021 · Biotechnology and bioengineering · DOI

Full title: Questioning coverage values determined by 2D western blots: A critical study on the characterization of anti-HCP ELISA reagents.

Authors: Seisenberger, Christina; Graf, Tobias; Haindl, Markus; Wegele, Harald; Wiedmann, Michael; Wohlrab, Stefanie

Year: 2021

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.27635

Paper Improving the analytical toolbox to investigate copurifying host cell proteins presence: N-(4)-(β-acetylglucosaminyl)- l-asparaginase case study.

Clavier, Séverine; Fougeron, Delphine; Petrovic, Suzana et al. · 2020 · Biotechnology and bioengineering · DOI

Full title: Improving the analytical toolbox to investigate copurifying host cell proteins presence: N-(4)-(β-acetylglucosaminyl)- l-asparaginase case study.

Authors: Clavier, Séverine; Fougeron, Delphine; Petrovic, Suzana; Elmaleh, Hagit; Fourneaux, Céline; Bugnazet, Dawid; Duffieux, Francis; Masiero, Alessandro; Mitra-Kaushik, Shibani; Genet, Bruno; Fromentin, Yann; Kreiss, Patrick; Laborderie, Bénédicte; Brault, Dominique; Menet, Jean-Michel

Year: 2020

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.27514

Paper Purification processes of live virus vaccine candidates expressed in adherent Vero cell lines via multimodal chromatography in flowthrough mode.

Konstantinidis, Spyridon; Poplyk, Murphy R; Ma, Wanli Justin et al. · 2024 · Biotechnology and bioengineering · DOI

Full title: Purification processes of live virus vaccine candidates expressed in adherent Vero cell lines via multimodal chromatography in flowthrough mode.

Authors: Konstantinidis, Spyridon; Poplyk, Murphy R; Ma, Wanli Justin; Reilly, Devan; Zhang, Young; Wang, Jamin; Thompson, Rachel; Stiving, Alyssa; Winters, Michael A; Wang, Sheng-Ching; Kristopeit, Adam

Year: 2024

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28430

Paper Comprehensive assessment of host cell protein expression after extended culture and bioreactor production of CHO cell lines.

Hamaker, Nathaniel K; Min, Lie; Lee, Kelvin H · 2022 · Biotechnology and bioengineering · DOI

Full title: Comprehensive assessment of host cell protein expression after extended culture and bioreactor production of CHO cell lines.

Authors: Hamaker, Nathaniel K; Min, Lie; Lee, Kelvin H

Year: 2022

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28128

Paper Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via "flow-through affinity chromatography" using peptide-based adsorbents.

Sripada, Sobhana Alekhya; Chu, Wenning; Williams, Taufika Islam et al. · 2022 · Biotechnology and bioengineering · DOI

Full title: Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via "flow-through affinity chromatography" using peptide-based adsorbents.

Authors: Sripada, Sobhana Alekhya; Chu, Wenning; Williams, Taufika Islam; Teten, Matthew A; Mosley, Brian J; Carbonell, Ruben G; Lenhoff, Abraham M; Cramer, Steven M; Bill, Jerome; Yigzaw, Yinges; Roush, David J; Menegatti, Stefano

Year: 2022

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28096

Paper Displacement to separate host-cell proteins and aggregates in cation-exchange chromatography of monoclonal antibodies.

Khanal, Ohnmar; Kumar, Vijesh; Lenhoff, Abraham M · 2021 · Biotechnology and bioengineering · DOI

Full title: Displacement to separate host-cell proteins and aggregates in cation-exchange chromatography of monoclonal antibodies.

Authors: Khanal, Ohnmar; Kumar, Vijesh; Lenhoff, Abraham M

Year: 2021

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.27559

Paper Characterization and implications of host-cell protein aggregates in biopharmaceutical processing.

Oh, Young Hoon; Becker, Matthew L; Mendola, Kerri M et al. · 2023 · Biotechnology and bioengineering · DOI

Full title: Characterization and implications of host-cell protein aggregates in biopharmaceutical processing.

Authors: Oh, Young Hoon; Becker, Matthew L; Mendola, Kerri M; Choe, Leila H; Min, Lie; Lee, Kelvin H; Yigzaw, Yinges; Seay, Alexander; Bill, Jerome; Li, Xuanwen; Roush, David J; Cramer, Steven M; Menegatti, Stefano; Lenhoff, Abraham M

Year: 2023

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28325

Paper "High-risk" host cell proteins (HCPs): A multi-company collaborative view.

Jones, Marisa; Palackal, Nisha; Wang, Fengqiang et al. · 2021 · Biotechnology and bioengineering · DOI

Full title: "High-risk" host cell proteins (HCPs): A multi-company collaborative view.

Authors: Jones, Marisa; Palackal, Nisha; Wang, Fengqiang; Gaza-Bulseco, Georgeen; Hurkmans, Karen; Zhao, Yiwei; Chitikila, Carmelata; Clavier, Severine; Liu, Suli; Menesale, Emily; Schonenbach, Nicole S; Sharma, Satish; Valax, Pascal; Waerner, Thomas; Zhang, Lei; Connolly, Trish

Year: 2021

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.27808

Paper Comparison of host cell protein removal by depth filters with diatomaceous earth and synthetic silica filter aids using model proteins.

Chu, Liang-Kai; Borujeni, Ehsan Espah; Xu, Xuankuo et al. · 2023 · Biotechnology and bioengineering · DOI

Full title: Comparison of host cell protein removal by depth filters with diatomaceous earth and synthetic silica filter aids using model proteins.

Authors: Chu, Liang-Kai; Borujeni, Ehsan Espah; Xu, Xuankuo; Ghose, Sanchayita; Zydney, Andrew L

Year: 2023

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28386

Paper High throughput screening for rapid and reliable prediction of monovalent antibody binding behavior in flowthrough mode

Julius Klemens Lorek; H. S. Karkov; F. Matthiesen et al. · 2023 · Biotechnology and Bioengineering · DOI

Full title: High throughput screening for rapid and reliable prediction of monovalent antibody binding behavior in flowthrough mode

Authors: Julius Klemens Lorek; H. S. Karkov; F. Matthiesen; Maria Dainiak

Year: 2023

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.28572

Paper Factors affecting product association as a mechanism of host-cell protein persistence in bioprocessing.

Oh, Young Hoon; Becker, Matthew L; Mendola, Kerri M et al. · 2024 · Biotechnology and bioengineering · DOI

Full title: Factors affecting product association as a mechanism of host-cell protein persistence in bioprocessing.

Year: 2024

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28658

Paper Identification and characterization of CHO host-cell proteins in monoclonal antibody bioprocessing.

Oh, Young Hoon; Mendola, Kerri M; Choe, Leila H et al. · 2024 · Biotechnology and bioengineering · DOI

Full title: Identification and characterization of CHO host-cell proteins in monoclonal antibody bioprocessing.

Authors: Oh, Young Hoon; Mendola, Kerri M; Choe, Leila H; Min, Lie; Lavoie, Ashton R; Sripada, Sobhana A; Williams, Taufika Islam; Lee, Kelvin H; Yigzaw, Yinges; Seay, Alexander; Bill, Jerome; Li, Xuanwen; Roush, David J; Cramer, Steven M; Menegatti, Stefano; Lenhoff, Abraham M

Year: 2024

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28568

Paper Holistic analytical characterization and risk assessment of residual host cell protein impurities in an active pharmaceutical ingredient synthesized by biocatalysts.

Wang, Fengqiang; Li, Xuanwen; Swanson, Michael et al. · 2022 · Biotechnology and bioengineering · DOI

Full title: Holistic analytical characterization and risk assessment of residual host cell protein impurities in an active pharmaceutical ingredient synthesized by biocatalysts.

Authors: Wang, Fengqiang; Li, Xuanwen; Swanson, Michael; Guetschow, Erik; Winston, Matthew; Smith, Joseph P; Hoyt, Erik; Liu, Zhijian; Richardson, Douglas; Bu, Xiaodong; Jawa, Vibha; Variankaval, Narayan

Year: 2022

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28112

Paper Host cell protein networks as a novel co-elution mechanism during protein A chromatography.

Panikulam, Sherin; Hanke, Alexander; Kroener, Frieder et al. · 2024 · Biotechnology and bioengineering · DOI

Full title: Host cell protein networks as a novel co-elution mechanism during protein A chromatography.

Authors: Panikulam, Sherin; Hanke, Alexander; Kroener, Frieder; Karle, Anette; Anderka, Oliver; Villiger, Thomas K; Lebesgue, Nicolas

Year: 2024

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28678

Paper Assessing the Adjuvant Potential of Chinese Hamster Ovary Host Cell Proteins Using an In Vitro Dendritic Cell Assay.

Panikulam, Sherin; Morgan, Hannah; Gutknecht, Michael et al. · 2025 · Biotechnology and bioengineering · DOI

Full title: Assessing the Adjuvant Potential of Chinese Hamster Ovary Host Cell Proteins Using an In Vitro Dendritic Cell Assay.

Authors: Panikulam, Sherin; Morgan, Hannah; Gutknecht, Michael; Karle, Anette; Rajaratnam, Atchaya; Muntwyler, Jennifer; Anderka, Oliver; Lebesgue, Nicolas; Villiger, Thomas K

Year: 2025

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28950

Paper Quantitative Analysis Reveals Hitchhiking Drives Polysorbate Hydrolase Persistence Via Host Cell Protein-Antibody Interactions.

Maier, Melanie; Griesinger, Lukas; Franzreb, Matthias et al. · 2026 · Biotechnology and bioengineering · DOI

Full title: Quantitative Analysis Reveals Hitchhiking Drives Polysorbate Hydrolase Persistence Via Host Cell Protein-Antibody Interactions.

Authors: Maier, Melanie; Griesinger, Lukas; Franzreb, Matthias; Kluters, Simon

Year: 2026

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.70170

Paper Integration of Single Pass Tangential Flow Filtration and High Performance Countercurrent Membrane Purification for Intensification of Monoclonal Antibody Processing.

Minervini, Mirko; Mohammadzadehmarandi, Aylin; Behboudi, Ali et al. · 2025 · Biotechnology and bioengineering · DOI

Full title: Integration of Single Pass Tangential Flow Filtration and High Performance Countercurrent Membrane Purification for Intensification of Monoclonal Antibody Processing.

Authors: Minervini, Mirko; Mohammadzadehmarandi, Aylin; Behboudi, Ali; Oppenheim, Sheldon F; Zydney, Andrew L

Year: 2025

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.29038

Paper Elucidation of Proteoforms of Chinese Hamster Ovary (CHO) Phospholipase B-Like 2 (PLBL2) Captured From a Monoclonal Antibody.

Dolan, Michael E; Wang, Lei Leo; Tedeschi, Alexander et al. · 2026 · Biotechnology and bioengineering · DOI

Full title: Elucidation of Proteoforms of Chinese Hamster Ovary (CHO) Phospholipase B-Like 2 (PLBL2) Captured From a Monoclonal Antibody.

Authors: Dolan, Michael E; Wang, Lei Leo; Tedeschi, Alexander; Wang, Yan; Barton, Christopher; Oppenheim, Sheldon F; Zhou, Zhaohui Sunny

Year: 2026

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.70104

Paper Tailoring polishing steps for effective removal of polysorbate-degrading host cell proteins in antibody purification.

Maier, Melanie; Schneider, Stefan; Weiss, Linus et al. · 2024 · Biotechnology and bioengineering · DOI

Full title: Tailoring polishing steps for effective removal of polysorbate-degrading host cell proteins in antibody purification.

Authors: Maier, Melanie; Schneider, Stefan; Weiss, Linus; Fischer, Simon; Lakatos, Daniel; Studts, Joey; Franzreb, Matthias

Year: 2024

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28767

Paper Profiling the effects of process changes on residual host cell proteins in biotherapeutics by mass spectrometry.

Schenauer, Matthew R; Flynn, Gregory C; Goetze, Andrew M · 2013 · Biotechnology progress · DOI

Full title: Profiling the effects of process changes on residual host cell proteins in biotherapeutics by mass spectrometry.

Authors: Schenauer, Matthew R; Flynn, Gregory C; Goetze, Andrew M

Year: 2013

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.1748

Paper Integrating Affinity Chromatography in the Platform Process for Adenovirus Purification.

Wu, Yuxuan; Barbieri, Eduardo; Smith, William K et al. · 2025 · Biotechnology and bioengineering · DOI

Full title: Integrating Affinity Chromatography in the Platform Process for Adenovirus Purification.

Authors: Wu, Yuxuan; Barbieri, Eduardo; Smith, William K; Minzoni, Arianna; Kilgore, Ryan E; Chu, Wenning; Daniele, Michael A; Menegatti, Stefano

Year: 2025

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.29006

Paper Buffer system improves the removal of host cell protein impurities in monoclonal antibody purification.

Lakatos, Dániel; Idler, Martina; Stibitzky, Selina et al. · 2024 · Biotechnology and bioengineering · DOI

Full title: Buffer system improves the removal of host cell protein impurities in monoclonal antibody purification.

Authors: Lakatos, Dániel; Idler, Martina; Stibitzky, Selina; Amann, Jennifer; Schuschkewitz, Jakob; Krayl, Dominik; Liebau, Judith; Grosch, Jan-Hendrik; Arango Gutierrez, Erik; Kluters, Simon

Year: 2024

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28844

Paper Multi-lipase gene knockdown in Chinese hamster ovary cells using artificial microRNAs to reduce host cell protein mediated polysorbate degradation.

Weiß, Linus; Schmieder-Todtenhaupt, Valerie; Haemmerling, Frank et al. · 2024 · Biotechnology and bioengineering · DOI

Full title: Multi-lipase gene knockdown in Chinese hamster ovary cells using artificial microRNAs to reduce host cell protein mediated polysorbate degradation.

Authors: Weiß, Linus; Schmieder-Todtenhaupt, Valerie; Haemmerling, Frank; Lakatos, Daniel; Schulz, Patrick; Fischer, Simon

Year: 2024

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28563

Paper Multiple Use of Regenerated Depth Filters in Antibody Purification Processes.

Spensberger, Bernhard; Stueckler, Ferdinand; Pompiati, Marc et al. · 2025 · Biotechnology and bioengineering · DOI

Full title: Multiple Use of Regenerated Depth Filters in Antibody Purification Processes.

Authors: Spensberger, Bernhard; Stueckler, Ferdinand; Pompiati, Marc; Feistl, Christoph; Lemm, Thorsten; Falkenstein, Roberto

Year: 2025

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28957

Paper Host cell protein adsorption characteristics during protein A chromatography.

Tarrant, Richard D R; Velez-Suberbie, M Lourdes; Tait, Andrew S et al. · 2012 · Biotechnology progress · DOI

Full title: Host cell protein adsorption characteristics during protein A chromatography.

Authors: Tarrant, Richard D R; Velez-Suberbie, M Lourdes; Tait, Andrew S; Smales, C Mark; Bracewell, Daniel G

Year: 2012

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.1581

Paper Differential Response in Downstream Processing of CHO Cells Grown Under Mild Hypothermic Conditions

A. Tait; R. Tarrant; M. Velez‐Suberbie et al. · 2013 · Biotechnology progress (Print) · DOI

Full title: Differential Response in Downstream Processing of CHO Cells Grown Under Mild Hypothermic Conditions

Authors: A. Tait; R. Tarrant; M. Velez‐Suberbie; D. Spencer; D. Bracewell

Year: 2013

Venue: Biotechnology progress (Print)

DOI: https://doi.org/10.1002/btpr.1726

Paper Matrix effects during monitoring of antibody and host cell proteins using attenuated total reflection spectroscopy.

Capito, Florian; Skudas, Romas; Stanislawski, Bernd et al. · 2013 · Biotechnology progress · DOI

Full title: Matrix effects during monitoring of antibody and host cell proteins using attenuated total reflection spectroscopy.

Authors: Capito, Florian; Skudas, Romas; Stanislawski, Bernd; Kolmar, Harald

Year: 2013

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.1643

Paper A novel approach to monitor clearance of host cell proteins associated with monoclonal antibodies.

Aboulaich, Nabila; Chung, Wai Keen; Thompson, Jenny Heidbrink et al. · 2014 · Biotechnology progress · DOI

Full title: A novel approach to monitor clearance of host cell proteins associated with monoclonal antibodies.

Authors: Aboulaich, Nabila; Chung, Wai Keen; Thompson, Jenny Heidbrink; Larkin, Christopher; Robbins, David; Zhu, Min

Year: 2014

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.1948

Paper Characterization of the co-elution of host cell proteins with monoclonal antibodies during protein A purification.

Zhang, Qingchun; Goetze, Andrew M; Cui, Huanchun et al. · 2016 · Biotechnology progress · DOI

Full title: Characterization of the co-elution of host cell proteins with monoclonal antibodies during protein A purification.

Authors: Zhang, Qingchun; Goetze, Andrew M; Cui, Huanchun; Wylie, Jenna; Tillotson, Ben; Hewig, Art; Hall, Michael P; Flynn, Gregory C

Year: 2016

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.2272

Paper Select host cell proteins coelute with monoclonal antibodies in protein A chromatography.

Nogal, Bartek; Chhiba, Krishan; Emery, Jefferson C · 2012 · Biotechnology progress · DOI

Full title: Select host cell proteins coelute with monoclonal antibodies in protein A chromatography.

Authors: Nogal, Bartek; Chhiba, Krishan; Emery, Jefferson C

Year: 2012

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.1514

Paper Trace levels of the CHO host cell protease cathepsin D caused particle formation in a monoclonal antibody product.

Bee, Jared S; Tie, Liu; Johnson, Douglas et al. · 2015 · Biotechnology progress · DOI

Full title: Trace levels of the CHO host cell protease cathepsin D caused particle formation in a monoclonal antibody product.

Authors: Bee, Jared S; Tie, Liu; Johnson, Douglas; Dimitrova, Mariana N; Jusino, Kristin C; Afdahl, Christopher D

Year: 2015

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.2150

Paper Development of advanced host cell protein enrichment and detection strategies to enable process relevant spike challenge studies

Soderquist, Ryan G.; Trumbo, Mihaela; Hart, Roger A. et al. · 2015 · Biotechnology Progress · DOI

Full title: Development of advanced host cell protein enrichment and detection strategies to enable process relevant spike challenge studies

Authors: Soderquist, Ryan G.; Trumbo, Mihaela; Hart, Roger A.; Zhang, Qingchun; Flynn, Gregory C.

Year: 2015

Venue: Biotechnology Progress

DOI: https://doi.org/10.1002/btpr.2114

Paper Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein release in C hinese hamster ovary cells

Migani, Damiano; Smales, C. Mark; Bracewell, Daniel G. · 2017 · Biotechnology Progress · DOI

Full title: Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein release in C hinese hamster ovary cells

Authors: Migani, Damiano; Smales, C. Mark; Bracewell, Daniel G.

Year: 2017

Venue: Biotechnology Progress

DOI: https://doi.org/10.1002/btpr.2455

Paper Accounting for host cell protein behavior in anion-exchange chromatography.

Swanson, Ryan K; Xu, Ruo; Nettleton, Daniel S et al. · 2016 · Biotechnology progress · DOI

Full title: Accounting for host cell protein behavior in anion-exchange chromatography.

Authors: Swanson, Ryan K; Xu, Ruo; Nettleton, Daniel S; Glatz, Charles E

Year: 2016

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.2342

Paper Caprylic acid-induced impurity precipitation from protein A capture column elution pool to enable a two-chromatography-step process for monoclonal antibody purification.

Zheng, Ji; Wang, Lu; Twarowska, Barbara et al. · 2015 · Biotechnology progress · DOI

Full title: Caprylic acid-induced impurity precipitation from protein A capture column elution pool to enable a two-chromatography-step process for monoclonal antibody purification.

Authors: Zheng, Ji; Wang, Lu; Twarowska, Barbara; Laino, Sarah; Sparks, Colleen; Smith, Timothy; Russell, Reb; Wang, Michelle

Year: 2015

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.2154

Paper Experience with host cell protein impurities in biopharmaceuticals.

Vanderlaan, Martin; Zhu-Shimoni, Judith; Lin, Sansan et al. · 2018 · Biotechnology progress · DOI

Full title: Experience with host cell protein impurities in biopharmaceuticals.

Authors: Vanderlaan, Martin; Zhu-Shimoni, Judith; Lin, Sansan; Gunawan, Feny; Waerner, Thomas; Van Cott, Kevin E

Year: 2018

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.2640

Paper Process characterization strategy for a precipitation step for host cell protein reduction.

Prentice, Jessica; Vu, Diemchi; Robbins, David et al. · 2020 · Biotechnology progress · DOI

Full title: Process characterization strategy for a precipitation step for host cell protein reduction.

Authors: Prentice, Jessica; Vu, Diemchi; Robbins, David; Ferreira, Gisela

Year: 2020

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.2908

Paper Understanding the mechanism of copurification of "difficult to remove" host cell proteins in rituximab biosimilar products.

Singh, Sumit K; Mishra, Avinash; Yadav, Divyanshi et al. · 2020 · Biotechnology progress · DOI

Full title: Understanding the mechanism of copurification of "difficult to remove" host cell proteins in rituximab biosimilar products.

Authors: Singh, Sumit K; Mishra, Avinash; Yadav, Divyanshi; Budholiya, Niharika; Rathore, Anurag S

Year: 2020

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.2936

Paper Effects of pH, conductivity, host cell protein, and DNA size distribution on DNA clearance in anion exchange chromatography media

Stone, Melani C.; Borman, Jon; Ferreira, Gisela et al. · 2017 · Biotechnology Progress · DOI

Full title: Effects of pH, conductivity, host cell protein, and DNA size distribution on DNA clearance in anion exchange chromatography media

Authors: Stone, Melani C.; Borman, Jon; Ferreira, Gisela; Robbins, Paul D.

Year: 2017

Venue: Biotechnology Progress

DOI: https://doi.org/10.1002/btpr.2556

Paper Mass spectrometric evaluation of upstream and downstream process influences on host cell protein patterns in biopharmaceutical products.

Falkenberg, Heiner; Waldera-Lupa, Daniel Michael; Vanderlaan, Martin et al. · 2019 · Biotechnology progress · DOI

Full title: Mass spectrometric evaluation of upstream and downstream process influences on host cell protein patterns in biopharmaceutical products.

Authors: Falkenberg, Heiner; Waldera-Lupa, Daniel Michael; Vanderlaan, Martin; Schwab, Thomas; Krapfenbauer, Kurt; Studts, Joey Michael; Flad, Thomas; Waerner, Thomas

Year: 2019

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.2788

Paper Identification and tracking of problematic host cell proteins removed by a synthetic, highly functionalized nonwoven media in downstream bioprocessing of monoclonal antibodies.

Gilgunn, S; El-Sabbahy, H; Albrecht, S et al. · 2019 · Journal of chromatography. A · DOI

Full title: Identification and tracking of problematic host cell proteins removed by a synthetic, highly functionalized nonwoven media in downstream bioprocessing of monoclonal antibodies.

Authors: Gilgunn, S; El-Sabbahy, H; Albrecht, S; Gaikwad, M; Corrigan, K; Deakin, L; Jellum, G; Bones, J

Year: 2019

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2019.02.056

Paper Precision analysis for the determination of steric mass action parameters using eight tobacco host cell proteins.

Bernau, C R; Jäpel, R C; Hübbers, J W et al. · 2021 · Journal of chromatography. A · DOI

Full title: Precision analysis for the determination of steric mass action parameters using eight tobacco host cell proteins.

Authors: Bernau, C R; Jäpel, R C; Hübbers, J W; Nölting, S; Opdensteinen, P; Buyel, J F

Year: 2021

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2021.462379

Paper Formation of transient highly-charged mAb clusters strengthens interactions with host cell proteins and results in poor clearance of host cell proteins by protein A chromatography.

Luo, Haibin; Du, Qun; Qian, Chen et al. · 2022 · Journal of chromatography. A · DOI

Full title: Formation of transient highly-charged mAb clusters strengthens interactions with host cell proteins and results in poor clearance of host cell proteins by protein A chromatography.

Authors: Luo, Haibin; Du, Qun; Qian, Chen; Mlynarczyk, Michelle; Pabst, Timothy M; Damschroder, Melissa; Hunter, Alan K; Wang, William K

Year: 2022

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2022.463385

Paper A novel aqueous two phase assisted platform for efficient removal of process related impurities associated with E. coli based biotherapeutic protein products.

Bhambure, Rahul; Sharma, Rohit; Gupta, Darpan et al. · 2013 · Journal of chromatography. A · DOI

Full title: A novel aqueous two phase assisted platform for efficient removal of process related impurities associated with E. coli based biotherapeutic protein products.

Authors: Bhambure, Rahul; Sharma, Rohit; Gupta, Darpan; Rathore, Anurag S

Year: 2013

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2013.07.085

Paper Immunoreactivity profiling of Anti-Chinese hamster ovarian host cell protein antibodies by isobaric labeled affinity purification-mass spectrometry reveals low-recovery proteins.

Takagi, Shunsuke; Shibata, Masayoshi; Suzuki, Nobuyuki et al. · 2022 · Journal of chromatography. A · DOI

Full title: Immunoreactivity profiling of Anti-Chinese hamster ovarian host cell protein antibodies by isobaric labeled affinity purification-mass spectrometry reveals low-recovery proteins.

Authors: Takagi, Shunsuke; Shibata, Masayoshi; Suzuki, Nobuyuki; Ishihama, Yasushi

Year: 2022

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2022.463645

Paper Exploring features in chromatographic profiles as a tool for monitoring column performance.

Ravi, Nivetita; Malmquist, Gunnar; Stanev, Valentin et al. · 2023 · Journal of chromatography. A · DOI

Full title: Exploring features in chromatographic profiles as a tool for monitoring column performance.

Authors: Ravi, Nivetita; Malmquist, Gunnar; Stanev, Valentin; Ferreira, Gisela

Year: 2023

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2023.463982

Paper Proteomics analysis of host cell proteins after immobilized metal affinity chromatography: Influence of ligand and metal ions.

Lingg, Nico; Öhlknecht, Christoph; Fischer, Andreas et al. · 2020 · Journal of chromatography. A · DOI

Full title: Proteomics analysis of host cell proteins after immobilized metal affinity chromatography: Influence of ligand and metal ions.

Authors: Lingg, Nico; Öhlknecht, Christoph; Fischer, Andreas; Mozgovicz, Markus; Scharl, Theresa; Oostenbrink, Chris; Jungbauer, Alois

Year: 2020

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2020.461649

Paper Behavior of host-cell-protein-rich aggregates in antibody capture and polishing chromatography.

Herman, Chase E; Min, Lie; Choe, Leila H et al. · 2023 · Journal of chromatography. A · DOI

Full title: Behavior of host-cell-protein-rich aggregates in antibody capture and polishing chromatography.

Authors: Herman, Chase E; Min, Lie; Choe, Leila H; Maurer, Ronald W; Xu, Xuankuo; Ghose, Sanchayita; Lee, Kelvin H; Lenhoff, Abraham M

Year: 2023

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2023.464081

Paper Biomimetic affinity chromatography for antibody purification: Host cell protein binding and impurity removal.

Huang, Haotian; Dong, Xiaoyan; Sun, Yan et al. · 2023 · Journal of chromatography. A · DOI

Full title: Biomimetic affinity chromatography for antibody purification: Host cell protein binding and impurity removal.

Authors: Huang, Haotian; Dong, Xiaoyan; Sun, Yan; Shi, Qinghong

Year: 2023

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2023.464305

Paper Leveraging prior knowledge for process parameter classification in mAb Protein A chromatography.

Tao, Yinying; Rauk, Adam; Gao, Jinxin et al. · 2025 · Journal of chromatography. A · DOI

Full title: Leveraging prior knowledge for process parameter classification in mAb Protein A chromatography.

Authors: Tao, Yinying; Rauk, Adam; Gao, Jinxin; De Felippis, Michael R

Year: 2025

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2024.465647

Paper Engineering protein A ligands to mitigate antibody loss during high-pH washes in protein A chromatography.

Bhoyar, Soumitra; Foster, Max; Oh, Young Hoon et al. · 2023 · Journal of chromatography. A · DOI

Full title: Engineering protein A ligands to mitigate antibody loss during high-pH washes in protein A chromatography.

Authors: Bhoyar, Soumitra; Foster, Max; Oh, Young Hoon; Xu, Xuankuo; Traylor, Steven J; Guo, Jing; Ghose, Sanchayita; Lenhoff, Abraham M

Year: 2023

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2023.463962

Paper Purification of a non-enveloped virus using sequential aqueous two-phase extraction.

Natalie M. Nold; Seth Kriz; Sheridan Waldack et al. · 2025 · Journal of Chromatography A · DOI

Full title: Purification of a non-enveloped virus using sequential aqueous two-phase extraction.

Authors: Natalie M. Nold; Seth Kriz; Sheridan Waldack; Grace James; Trisha Colling; Taravat Sarvari; Vaishali Sharma; Alexis Pohkrel; Ethan Burghardt; Pratik U. Joshi; C. Heldt

Year: 2025

Venue: Journal of Chromatography A

DOI: https://doi.org/10.1016/j.chroma.2025.465866

Paper Hydrophobicity analysis of Chinese Hamster Ovary host cell proteins using analytical hydrophobic interaction chromatography and LC-MS.

Chu, Liang-Kai; Laremore, Tatiana; Borujeni, Ehsan Espah et al. · 2025 · Journal of chromatography. A · DOI

Full title: Hydrophobicity analysis of Chinese Hamster Ovary host cell proteins using analytical hydrophobic interaction chromatography and LC-MS.

Authors: Chu, Liang-Kai; Laremore, Tatiana; Borujeni, Ehsan Espah; Xu, Xuankuo; Zydney, Andrew L

Year: 2025

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2025.466440

Paper Impact of ligand structure and base bead pore size on host cell protein removal during monoclonal antibody purification using multimodal chromatography resin.

Mori, Chigusa; Iwamoto, Eri; Kadoi, Kenji et al. · 2024 · Journal of chromatography. A · DOI

Full title: Impact of ligand structure and base bead pore size on host cell protein removal during monoclonal antibody purification using multimodal chromatography resin.

Authors: Mori, Chigusa; Iwamoto, Eri; Kadoi, Kenji; Pluskal, Malcolm G; Matsumoto, Yoshihiro

Year: 2024

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2024.465202

Paper Improved clearance of host cell protein impurities at the polishing purification step using multimodal chromatography.

Hutchinson, Jack; Lu, Yali; Grew, Lara et al. · 2024 · Journal of chromatography. A · DOI

Full title: Improved clearance of host cell protein impurities at the polishing purification step using multimodal chromatography.

Authors: Hutchinson, Jack; Lu, Yali; Grew, Lara; Cui, Tingting

Year: 2024

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2024.465229

Paper Applications of proteomic methods for CHO host cell protein characterization in biopharmaceutical manufacturing.

Valente, Kristin N; Levy, Nicholas E; Lee, Kelvin H et al. · 2018 · Current opinion in biotechnology · DOI

Full title: Applications of proteomic methods for CHO host cell protein characterization in biopharmaceutical manufacturing.

Authors: Valente, Kristin N; Levy, Nicholas E; Lee, Kelvin H; Lenhoff, Abraham M

Year: 2018

Venue: Current opinion in biotechnology

DOI: https://doi.org/10.1016/j.copbio.2018.01.004

Paper Multi-component behavior of host-cell protein- and antibody-containing heteroaggregates in protein A chromatography.

Bhoyar, Soumitra; Foster, Max; Lenhoff, Abraham M · 2025 · Journal of chromatography. A · DOI

Full title: Multi-component behavior of host-cell protein- and antibody-containing heteroaggregates in protein A chromatography.

Authors: Bhoyar, Soumitra; Foster, Max; Lenhoff, Abraham M

Year: 2025

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2025.465954

Paper Measurement and control of host cell proteins (HCPs) in CHO cell bioprocesses.

Hogwood, Catherine E M; Bracewell, Daniel G; Smales, C Mark · 2014 · Current opinion in biotechnology · DOI

Full title: Measurement and control of host cell proteins (HCPs) in CHO cell bioprocesses.

Authors: Hogwood, Catherine E M; Bracewell, Daniel G; Smales, C Mark

Year: 2014

Venue: Current opinion in biotechnology

DOI: https://doi.org/10.1016/j.copbio.2014.06.017

Paper In-house CHO HCPs platform: A promising approach for HCPs ELISA monitoring.

Giordano, Elisa; Liori, Barbara; Cecchini, Irene et al. · 2024 · European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences · DOI

Full title: In-house CHO HCPs platform: A promising approach for HCPs ELISA monitoring.

Authors: Giordano, Elisa; Liori, Barbara; Cecchini, Irene; Verani, Roberta; Leone, Lidia

Year: 2024

Venue: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

DOI: https://doi.org/10.1016/j.ejps.2023.106656

Paper Mitigating polysorbate degradation-induced visible particle formation in an antibody formulation: From root cause identification to strategic solution.

Hu, Ting; Yi, Meiqi; Zhang, Zheng et al. · 2025 · International journal of pharmaceutics · DOI

Full title: Mitigating polysorbate degradation-induced visible particle formation in an antibody formulation: From root cause identification to strategic solution.

Authors: Hu, Ting; Yi, Meiqi; Zhang, Zheng; Guo, Xuefeng; Fan, Yamin; Duan, Tingting; Li, Xinchao; Zhang, Yuqi; Kang, Yanshen; Jiang, Wang; Chen, Zeyu; Chen, Shi; Song, Jing

Year: 2025

Venue: International journal of pharmaceutics

DOI: https://doi.org/10.1016/j.ijpharm.2025.126311

Paper Application of the Protein Maker as a platform purification system for therapeutic antibody research and development.

Hélie, Geneviève; Parat, Marie; Massé, Frédéric et al. · 2016 · Computational and structural biotechnology journal · DOI

Full title: Application of the Protein Maker as a platform purification system for therapeutic antibody research and development.

Authors: Hélie, Geneviève; Parat, Marie; Massé, Frédéric; Gerdts, Cory J; Loisel, Thomas P; Matte, Allan

Year: 2016

Venue: Computational and structural biotechnology journal

DOI: https://doi.org/10.1016/j.csbj.2016.06.001

Paper Assessing the polysorbate degradation fingerprints and kinetics of lipases - how the activity of polysorbate degrading hydrolases is influenced by the assay and assay conditions.

Glücklich, Nils; Carle, Stefan; Buske, Julia et al. · 2021 · European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences · DOI

Full title: Assessing the polysorbate degradation fingerprints and kinetics of lipases - how the activity of polysorbate degrading hydrolases is influenced by the assay and assay conditions.

Authors: Glücklich, Nils; Carle, Stefan; Buske, Julia; Mäder, Karsten; Garidel, Patrick

Year: 2021

Venue: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

DOI: https://doi.org/10.1016/j.ejps.2021.105980

Paper Sorbitol/methanol mixed induction reduces process impurities and improves centrifugal dewatering in Pichia pastoris culture.

Wang, Baolong; Nesbeth, Darren; Keshavarz-Moore, Eli · 2019 · Enzyme and microbial technology · DOI

Full title: Sorbitol/methanol mixed induction reduces process impurities and improves centrifugal dewatering in Pichia pastoris culture.

Authors: Wang, Baolong; Nesbeth, Darren; Keshavarz-Moore, Eli

Year: 2019

Venue: Enzyme and microbial technology

DOI: https://doi.org/10.1016/j.enzmictec.2019.109366

Paper SP3-based host cell protein monitoring in AAV-based gene therapy products using LC-MS/MS.

Smith, Josh; Strasser, Lisa; Guapo, Felipe et al. · 2023 · European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V · DOI

Full title: SP3-based host cell protein monitoring in AAV-based gene therapy products using LC-MS/MS.

Authors: Smith, Josh; Strasser, Lisa; Guapo, Felipe; Milian, Steven G; Snyder, Richard O; Bones, Jonathan

Year: 2023

Venue: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

DOI: https://doi.org/10.1016/j.ejpb.2023.06.019

Paper Integrated strategy for deep profiling of host cell proteins in downstream processing of therapeutic monoclonal antibodies: Novel approach to isolate and digest host cell proteins.

Zarei, Mostafa; Jonveaux, Jérôme; Razvi, Abbas et al. · 2024 · European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V · DOI

Full title: Integrated strategy for deep profiling of host cell proteins in downstream processing of therapeutic monoclonal antibodies: Novel approach to isolate and digest host cell proteins.

Authors: Zarei, Mostafa; Jonveaux, Jérôme; Razvi, Abbas; Jahn, Michael

Year: 2024

Venue: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

DOI: https://doi.org/10.1016/j.ejpb.2024.114369

Paper Cation exchange versus multimodal cation exchange resins for antibody capture from CHO supernatants: identification of contaminating host cell proteins by mass spectrometry.

Joucla, G; Le Sénéchal, C; Bégorre, M et al. · 2013 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · DOI

Full title: Cation exchange versus multimodal cation exchange resins for antibody capture from CHO supernatants: identification of contaminating host cell proteins by mass spectrometry.

Authors: Joucla, G; Le Sénéchal, C; Bégorre, M; Garbay, B; Santarelli, X; Cabanne, C

Year: 2013

Venue: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI: https://doi.org/10.1016/j.jchromb.2013.10.033

Paper Cathepsin D: Removal strategy on protein A chromatography, near real time monitoring and characterisation during monoclonal antibody production.

Cui, Tingting; Chi, Bertie; Heidbrink Thompson, Jenny et al. · 2019 · Journal of biotechnology · DOI

Full title: Cathepsin D: Removal strategy on protein A chromatography, near real time monitoring and characterisation during monoclonal antibody production.

Authors: Cui, Tingting; Chi, Bertie; Heidbrink Thompson, Jenny; Kasali, Toyin; Sellick, Christopher; Turner, Richard

Year: 2019

Venue: Journal of biotechnology

DOI: https://doi.org/10.1016/j.jbiotec.2019.08.013

Paper Cation exchange chromatography in antibody purification: pH screening for optimised binding and HCP removal.

Stein, Andreas; Kiesewetter, André · 2007 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · DOI

Full title: Cation exchange chromatography in antibody purification: pH screening for optimised binding and HCP removal.

Authors: Stein, Andreas; Kiesewetter, André

Year: 2007

Venue: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI: https://doi.org/10.1016/j.jchromb.2006.10.010

Paper SWATH-MS as a strategy for CHO host cell protein identification and quantification supporting the characterization of mAb purification platforms.

Carvalho, Sofia B; Profit, Ludivine; Krishnan, Sushmitha et al. · 2024 · Journal of biotechnology · DOI

Full title: SWATH-MS as a strategy for CHO host cell protein identification and quantification supporting the characterization of mAb purification platforms.

Authors: Carvalho, Sofia B; Profit, Ludivine; Krishnan, Sushmitha; Gomes, Ricardo A; Alexandre, Bruno M; Clavier, Severine; Hoffman, Michael; Brower, Kevin; Gomes-Alves, Patrícia

Year: 2024

Venue: Journal of biotechnology

DOI: https://doi.org/10.1016/j.jbiotec.2024.02.001

Paper Host cell protein identification in monoclonal antibody high molecular weight species.

Hu, Mengqi; Molden, Rosalynn; Hu, Yunli et al. · 2022 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · DOI

Full title: Host cell protein identification in monoclonal antibody high molecular weight species.

Authors: Hu, Mengqi; Molden, Rosalynn; Hu, Yunli; Huang, Yu; Qiu, Haibo; Li, Ning

Year: 2022

Venue: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI: https://doi.org/10.1016/j.jchromb.2022.123448

Paper Factors affecting robustness of anion exchange chromatography: Selective retention of minute virus of mice using membrane media.

Chen, Shu-Ting; Xu, Wenbo; Cai, Kang et al. · 2022 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · DOI

Full title: Factors affecting robustness of anion exchange chromatography: Selective retention of minute virus of mice using membrane media.

Authors: Chen, Shu-Ting; Xu, Wenbo; Cai, Kang; Ferreira, Gisela; Ranil Wickramasinghe, S; Qian, Xianghong

Year: 2022

Venue: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI: https://doi.org/10.1016/j.jchromb.2022.123449

Paper Monoclonal antibody purification using activated carbon as a replacement for Protein A affinity chromatography.

Ishihara, Takashi; Miyahara, Maki; Yamamoto, Koichi · 2018 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · DOI

Full title: Monoclonal antibody purification using activated carbon as a replacement for Protein A affinity chromatography.

Authors: Ishihara, Takashi; Miyahara, Maki; Yamamoto, Koichi

Year: 2018

Venue: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI: https://doi.org/10.1016/j.jchromb.2018.10.004

Paper Purification of antibodies by precipitating impurities using Polyethylene Glycol to enable a two chromatography step process.

Giese, Glen; Myrold, Adam; Gorrell, Jeffrey et al. · 2013 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · DOI

Full title: Purification of antibodies by precipitating impurities using Polyethylene Glycol to enable a two chromatography step process.

Authors: Giese, Glen; Myrold, Adam; Gorrell, Jeffrey; Persson, Josefine

Year: 2013

Venue: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI: https://doi.org/10.1016/j.jchromb.2013.08.029

Paper Combined Protein A and size exclusion high performance liquid chromatography for the single-step measurement of mAb, aggregates and host cell proteins.

Gjoka, Xhorxhi; Schofield, Mark; Cvetkovic, Aleksandar et al. · 2014 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · DOI

Full title: Combined Protein A and size exclusion high performance liquid chromatography for the single-step measurement of mAb, aggregates and host cell proteins.

Authors: Gjoka, Xhorxhi; Schofield, Mark; Cvetkovic, Aleksandar; Gantier, Rene

Year: 2014

Venue: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI: https://doi.org/10.1016/j.jchromb.2014.09.017

Paper Improving impurities clearance by amino acids addition to buffer solutions for chromatographic purifications of monoclonal antibodies.

Ishihara, Takashi; Hosono, Mareto · 2015 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · DOI

Full title: Improving impurities clearance by amino acids addition to buffer solutions for chromatographic purifications of monoclonal antibodies.

Authors: Ishihara, Takashi; Hosono, Mareto

Year: 2015

Venue: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI: https://doi.org/10.1016/j.jchromb.2015.05.018

Paper A combination of multiple LC-MS approaches for the comprehensive characterization of recombinant herpes zoster vaccine.

Long, Zhen; Wei, Chen; Zhu, Xiang et al. · 2025 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · DOI

Full title: A combination of multiple LC-MS approaches for the comprehensive characterization of recombinant herpes zoster vaccine.

Authors: Long, Zhen; Wei, Chen; Zhu, Xiang; Luo, Xi; Li, Xiangjun; Zhao, Suli

Year: 2025

Venue: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI: https://doi.org/10.1016/j.jchromb.2025.124712

Paper Application of protein A-modified capillary-channeled polymer polypropylene fibers to the quantitation of IgG in complex matrices.

Trang, Hung K; Marcus, R Kenneth · 2017 · Journal of pharmaceutical and biomedical analysis · DOI

Full title: Application of protein A-modified capillary-channeled polymer polypropylene fibers to the quantitation of IgG in complex matrices.

Authors: Trang, Hung K; Marcus, R Kenneth

Year: 2017

Venue: Journal of pharmaceutical and biomedical analysis

DOI: https://doi.org/10.1016/j.jpba.2017.04.028

Paper Identification and characterization of co-purifying CHO host cell proteins in monoclonal antibody purification process.

Liu, Xinrong; Chen, Ying; Zhao, Yiwei et al. · 2019 · Journal of pharmaceutical and biomedical analysis · DOI

Full title: Identification and characterization of co-purifying CHO host cell proteins in monoclonal antibody purification process.

Authors: Liu, Xinrong; Chen, Ying; Zhao, Yiwei; Liu-Compton, Virginia; Chen, Wesley; Payne, Gillian; Lazar, Alexandru C

Year: 2019

Venue: Journal of pharmaceutical and biomedical analysis

DOI: https://doi.org/10.1016/j.jpba.2019.06.021

Paper Toward unbiased identification and comparative quantification of host cell protein impurities by automated iterative LC-MS/MS (HCP-AIMS) for therapeutic protein development.

Huang, Yu; Molden, Rosalynn; Hu, Mengqi et al. · 2021 · Journal of pharmaceutical and biomedical analysis · DOI

Full title: Toward unbiased identification and comparative quantification of host cell protein impurities by automated iterative LC-MS/MS (HCP-AIMS) for therapeutic protein development.

Authors: Huang, Yu; Molden, Rosalynn; Hu, Mengqi; Qiu, Haibo; Li, Ning

Year: 2021

Venue: Journal of pharmaceutical and biomedical analysis

DOI: https://doi.org/10.1016/j.jpba.2021.114069

Paper Identification of a host cell protein impurity in therapeutic protein, P1.

Ahluwalia, Deepti; Dhillon, Harbhajan; Slaney, Thomas et al. · 2017 · Journal of pharmaceutical and biomedical analysis · DOI

Full title: Identification of a host cell protein impurity in therapeutic protein, P1.

Authors: Ahluwalia, Deepti; Dhillon, Harbhajan; Slaney, Thomas; Song, Hangtian; Boux, Heather; Mehta, Sheetal; Zhang, Lei; Valdez, Anulfo; Krishnamurthy, Girija

Year: 2017

Venue: Journal of pharmaceutical and biomedical analysis

DOI: https://doi.org/10.1016/j.jpba.2017.03.065

Paper Host cell protein quantitation by LC-MS. Experimental demonstration, qualification, and comparison of methods in USP 1132.1.

Chrone, Victor G; Blaszczyk, Anthony J; Zhang, Derrick H et al. · 2025 · Journal of pharmaceutical and biomedical analysis · DOI

Full title: Host cell protein quantitation by LC-MS. Experimental demonstration, qualification, and comparison of methods in USP 1132.1.

Authors: Chrone, Victor G; Blaszczyk, Anthony J; Zhang, Derrick H; Nielsen, Solveig B; Crawford, Janne; Schwämmle, Veit; Højrup, Peter; Kofoed, Thomas; Peckham, Niomi; Mørtz, Ejvind

Year: 2025

Venue: Journal of pharmaceutical and biomedical analysis

DOI: https://doi.org/10.1016/j.jpba.2025.117051

Paper Host cell protein quantification of an optimized purification method by mass spectrometry.

Reiter, Karine; Suzuki, Motoshi; Olano, Lisa Renee et al. · 2019 · Journal of pharmaceutical and biomedical analysis · DOI

Full title: Host cell protein quantification of an optimized purification method by mass spectrometry.

Authors: Reiter, Karine; Suzuki, Motoshi; Olano, Lisa Renee; Narum, David L

Year: 2019

Venue: Journal of pharmaceutical and biomedical analysis

DOI: https://doi.org/10.1016/j.jpba.2019.06.038

Paper l-Asparaginase and HCP quantification by SWATH LC-MS/MS for new and improved purification step in Erwinia chrysanthemil-asparaginase manufacture.

Modi, Tapasvi; Regufe da Mota, Sergio; Gervais, David · 2022 · Journal of pharmaceutical and biomedical analysis · DOI

Full title: l-Asparaginase and HCP quantification by SWATH LC-MS/MS for new and improved purification step in Erwinia chrysanthemil-asparaginase manufacture.

Authors: Modi, Tapasvi; Regufe da Mota, Sergio; Gervais, David

Year: 2022

Venue: Journal of pharmaceutical and biomedical analysis

DOI: https://doi.org/10.1016/j.jpba.2021.114537

Paper Quantitation of host cell proteins in biopharmaceuticals from chinese hamster ovarian and vero cell lines using capillary electrophoresis western blots.

Pearson, Cara; Wang, Yi; Alkurdi, Eman et al. · 2023 · Journal of pharmaceutical and biomedical analysis · DOI

Full title: Quantitation of host cell proteins in biopharmaceuticals from chinese hamster ovarian and vero cell lines using capillary electrophoresis western blots.

Authors: Pearson, Cara; Wang, Yi; Alkurdi, Eman; Zhang, Young; Yin, Kuo; Rustandi, Richard R; Loughney, John W

Year: 2023

Venue: Journal of pharmaceutical and biomedical analysis

DOI: https://doi.org/10.1016/j.jpba.2023.115420

Paper Comparison of analytical-flow, micro-flow and nano-flow LC-MS/MS for sub-proteome analysis.

Zhen Long; Zixi Zhao; Xingliang Fan et al. · 2024 · Journal of Pharmaceutical and Biomedical Analysis · DOI

Full title: Comparison of analytical-flow, micro-flow and nano-flow LC-MS/MS for sub-proteome analysis.

Authors: Zhen Long; Zixi Zhao; Xingliang Fan; Xi Luo

Year: 2024

Venue: Journal of Pharmaceutical and Biomedical Analysis

DOI: https://doi.org/10.1016/j.jpba.2024.116484

Paper Host cell protein quantification workflow using optimized standards combined with data-independent acquisition mass spectrometry.

Hessmann, Steve; Chery, Cyrille; Sikora, Anne-Sophie et al. · 2023 · Journal of pharmaceutical analysis · DOI

Full title: Host cell protein quantification workflow using optimized standards combined with data-independent acquisition mass spectrometry.

Authors: Hessmann, Steve; Chery, Cyrille; Sikora, Anne-Sophie; Gervais, Annick; Carapito, Christine

Year: 2023

Venue: Journal of pharmaceutical analysis

DOI: https://doi.org/10.1016/j.jpha.2023.03.009

Paper A custom-made time-resolved fluoroimmunoassay for the quantitation of the host cell protein of Vero in rabies vaccine.

Yang, Yiqi; Li, Zhaoyue; Zhang, Zhigao et al. · 2023 · Journal of virological methods · DOI

Full title: A custom-made time-resolved fluoroimmunoassay for the quantitation of the host cell protein of Vero in rabies vaccine.

Authors: Yang, Yiqi; Li, Zhaoyue; Zhang, Zhigao; Zhai, Xiangming; Li, Xijiu; Cao, Yue; Fang, Haolin; He, Chunhui; Wu, Yingsong; Lin, Guanfeng

Year: 2023

Venue: Journal of virological methods

DOI: https://doi.org/10.1016/j.jviromet.2023.114752

Paper Improved identification of host cell proteins in a protein biopharmaceutical by LC-MS/MS using the ProteoMiner™ Enrichment Kit.

Mörtstedt, Harriet; Makower, Åsa; Edlund, Per-Olof et al. · 2020 · Journal of pharmaceutical and biomedical analysis · DOI

Full title: Improved identification of host cell proteins in a protein biopharmaceutical by LC-MS/MS using the ProteoMiner™ Enrichment Kit.

Authors: Mörtstedt, Harriet; Makower, Åsa; Edlund, Per-Olof; Sjöberg, Katarina; Tjernberg, Agneta

Year: 2020

Venue: Journal of pharmaceutical and biomedical analysis

DOI: https://doi.org/10.1016/j.jpba.2020.113256

Paper Detection and quantitation of host cell proteins in monoclonal antibody drug products using automated sample preparation and data-independent acquisition LC-MS/MS.

Strasser, Lisa; Oliviero, Giorgio; Jakes, Craig et al. · 2021 · Journal of pharmaceutical analysis · DOI

Full title: Detection and quantitation of host cell proteins in monoclonal antibody drug products using automated sample preparation and data-independent acquisition LC-MS/MS.

Authors: Strasser, Lisa; Oliviero, Giorgio; Jakes, Craig; Zaborowska, Izabela; Floris, Patrick; Ribeiro da Silva, Meire; Füssl, Florian; Carillo, Sara; Bones, Jonathan

Year: 2021

Venue: Journal of pharmaceutical analysis

DOI: https://doi.org/10.1016/j.jpha.2021.05.002

Paper Label-free shotgun proteomics: Exploiting a reliable and sensitive method to monitor residual host-cell proteins in monoclonal antibody products

Khalil, Somar; Wychowski, Adeline; Chéry, Cyrille C. et al. · 2023 · Journal of Pharmaceutical and Biomedical Analysis Open · DOI

Full title: Label-free shotgun proteomics: Exploiting a reliable and sensitive method to monitor residual host-cell proteins in monoclonal antibody products

Authors: Khalil, Somar; Wychowski, Adeline; Chéry, Cyrille C.; Gervais, Annick

Year: 2023

Venue: Journal of Pharmaceutical and Biomedical Analysis Open

DOI: https://doi.org/10.1016/j.jpbao.2023.100012

Paper Automated optimization of resin selection, wash parameters, and elution parameters for protein A-based purifications.

Anderson, Gracie K; Hicks, Mark; Hamlyn, Penny et al. · 2026 · New biotechnology · DOI

Full title: Automated optimization of resin selection, wash parameters, and elution parameters for protein A-based purifications.

Authors: Anderson, Gracie K; Hicks, Mark; Hamlyn, Penny; Lee, L Andrew; Kates, Patrick A

Year: 2026

Venue: New biotechnology

DOI: https://doi.org/10.1016/j.nbt.2025.11.004

Paper Characterization of difficult-to-remove host cell proteins in adeno-associated virus downstream processing.

Leibiger, Thomas M; Min, Lie; Lee, Kelvin H · 2025 · Molecular therapy. Methods & clinical development · DOI

Full title: Characterization of difficult-to-remove host cell proteins in adeno-associated virus downstream processing.

Authors: Leibiger, Thomas M; Min, Lie; Lee, Kelvin H

Year: 2025

Venue: Molecular therapy. Methods & clinical development

DOI: https://doi.org/10.1016/j.omtm.2025.101623

Paper Effective strategies for host cell protein clearance in downstream processing of monoclonal antibodies and Fc-fusion proteins.

Li, Yifeng · 2017 · Protein expression and purification · DOI

Full title: Effective strategies for host cell protein clearance in downstream processing of monoclonal antibodies and Fc-fusion proteins.

Authors: Li, Yifeng

Year: 2017

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2017.04.006

Paper Quantitative proteomic analysis of residual host cell protein retention across adeno-associated virus affinity chromatography.

Leibiger, Thomas M; Min, Lie; Lee, Kelvin H · 2024 · Molecular therapy. Methods & clinical development · DOI

Full title: Quantitative proteomic analysis of residual host cell protein retention across adeno-associated virus affinity chromatography.

Authors: Leibiger, Thomas M; Min, Lie; Lee, Kelvin H

Year: 2024

Venue: Molecular therapy. Methods & clinical development

DOI: https://doi.org/10.1016/j.omtm.2024.101383

Paper Beyond viability: Advancing CHO cell culture process strategies to modulate host cell protein levels.

Sahoo, Ansuman; Devenney, Kyle; Nicolas, Marcus-Allen et al. · 2024 · New biotechnology · DOI

Full title: Beyond viability: Advancing CHO cell culture process strategies to modulate host cell protein levels.

Authors: Sahoo, Ansuman; Devenney, Kyle; Nicolas, Marcus-Allen; Zill, Nicholas; Gupta, Balrina; Madabhushi, Sri Ranganayaki

Year: 2024

Venue: New biotechnology

DOI: https://doi.org/10.1016/j.nbt.2024.10.003

Paper Development of a novel engineered E. coli host cell line platform with improved column capacity performance for ion-exchange chromatography.

Mukherjee, Rudra Palash; Fruchtl, McKinzie S; Beitle, Robert R et al. · 2018 · Protein expression and purification · DOI

Full title: Development of a novel engineered E. coli host cell line platform with improved column capacity performance for ion-exchange chromatography.

Authors: Mukherjee, Rudra Palash; Fruchtl, McKinzie S; Beitle, Robert R; Brune, Ellen M

Year: 2018

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2017.09.012

Paper Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins: A case study of a bacterially expressed recombinant biopharmaceutical protein.

Heissel, Søren; Bunkenborg, Jakob; Kristiansen, Max Per et al. · 2018 · Protein expression and purification · DOI

Full title: Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins: A case study of a bacterially expressed recombinant biopharmaceutical protein.

Authors: Heissel, Søren; Bunkenborg, Jakob; Kristiansen, Max Per; Holmbjerg, Anne Fich; Grimstrup, Marie; Mørtz, Ejvind; Kofoed, Thomas; Højrup, Peter

Year: 2018

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2018.03.002

Paper Sodium caprylate induced precipitation post Protein A chromatography as an effective means for host cell protein clearance.

Wan, Yan; Zhang, Ting; Chen, Tao et al. · 2019 · Protein expression and purification · DOI

Full title: Sodium caprylate induced precipitation post Protein A chromatography as an effective means for host cell protein clearance.

Authors: Wan, Yan; Zhang, Ting; Chen, Tao; Wang, Ying; Li, Yifeng

Year: 2019

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2019.105460

Paper pH conditioning is a crucial step in primary recovery - A case study for a recombinant Fab from E. coli.

Gundinger, Thomas; Spadiut, Oliver · 2020 · Protein expression and purification · DOI

Full title: pH conditioning is a crucial step in primary recovery - A case study for a recombinant Fab from E. coli.

Authors: Gundinger, Thomas; Spadiut, Oliver

Year: 2020

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2019.105504

Paper A two-step process for capture and purification of human basic fibroblast growth factor from E. coli homogenate: Yield versus endotoxin clearance.

Sauer, Dominik Georg; Mosor, Magdalena; Frank, Anna-Carina et al. · 2019 · Protein expression and purification · DOI

Full title: A two-step process for capture and purification of human basic fibroblast growth factor from E. coli homogenate: Yield versus endotoxin clearance.

Authors: Sauer, Dominik Georg; Mosor, Magdalena; Frank, Anna-Carina; Weiß, Florian; Christler, Anna; Walch, Nicole; Jungbauer, Alois; Dürauer, Astrid

Year: 2019

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2018.08.009

Paper Promiscuity of host cell proteins in the purification of histidine tagged recombinant xylanase A by IMAC procedures: A case study with a Ni2+-tacn-based IMAC system.

Yang, Yuanzhong; Mitri, Khosse; Zhang, Chunfang et al. · 2019 · Protein expression and purification · DOI

Full title: Promiscuity of host cell proteins in the purification of histidine tagged recombinant xylanase A by IMAC procedures: A case study with a Ni2+-tacn-based IMAC system.

Authors: Yang, Yuanzhong; Mitri, Khosse; Zhang, Chunfang; Boysen, Reinhard I; Hearn, Milton T W

Year: 2019

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2019.05.009

Paper Evaluating the efficacy of immobilized metal affinity chromatography (IMAC) for host cell protein (HCP) removal from anti-HER2 scFv expressed in Escherichia coli.

Soltaninasab, Saba; Ahmadzadeh, Maryam; Shahhosseini, Soraya et al. · 2022 · Protein expression and purification · DOI

Full title: Evaluating the efficacy of immobilized metal affinity chromatography (IMAC) for host cell protein (HCP) removal from anti-HER2 scFv expressed in Escherichia coli.

Authors: Soltaninasab, Saba; Ahmadzadeh, Maryam; Shahhosseini, Soraya; Mohit, Elham

Year: 2022

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2021.106004

Paper Removal of polysorbates degradation-related impurities by depth filter screening: A case study.

Xuan, Chunxiao; Xu, Yangguang; Cui, Xinang et al. · 2026 · Protein expression and purification · DOI

Full title: Removal of polysorbates degradation-related impurities by depth filter screening: A case study.

Authors: Xuan, Chunxiao; Xu, Yangguang; Cui, Xinang; Ma, Tengfei; Lv, Fengjuan

Year: 2026

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2026.106893

Paper The advantage of high pH eluting Protein A resins over their regular counterparts in aggregate and host cell protein clearance.

Li, Ziyang; Hu, Lixia; Li, Yifeng · 2026 · Protein expression and purification · DOI

Full title: The advantage of high pH eluting Protein A resins over their regular counterparts in aggregate and host cell protein clearance.

Authors: Li, Ziyang; Hu, Lixia; Li, Yifeng

Year: 2026

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2025.106859

Paper The adequate amount of sodium chloride in Protein A wash buffer for effective host cell protein clearance.

Han, Jinyu; Yang, Ju; Wang, Ying et al. · 2019 · Protein expression and purification · DOI

Full title: The adequate amount of sodium chloride in Protein A wash buffer for effective host cell protein clearance.

Authors: Han, Jinyu; Yang, Ju; Wang, Ying; Li, Yifeng

Year: 2019

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2019.02.016

Paper Two major mechanisms contributing to copurification of CHO host cell proteins and strategies to minimize their negative impact.

Li, Yifeng · 2022 · Protein expression and purification · DOI

Full title: Two major mechanisms contributing to copurification of CHO host cell proteins and strategies to minimize their negative impact.

Authors: Li, Yifeng

Year: 2022

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2022.106113

Paper Deciphering the inconsistent performance of Protein A membrane on host cell protein clearance.

Yang, Yifan; Zhang, Xudong; Li, Yifeng · 2026 · Protein expression and purification · DOI

Full title: Deciphering the inconsistent performance of Protein A membrane on host cell protein clearance.

Authors: Yang, Yifan; Zhang, Xudong; Li, Yifeng

Year: 2026

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2026.106891

Paper Integrated native digestion and molecular weight cutoff enrichment with DIA-MS: a sensitive and selective approach for quantitative analysis of HCP.

Gong, Yachuan; Zhao, Yanjia; Peng, Rui et al. · 2026 · Talanta · DOI

Full title: Integrated native digestion and molecular weight cutoff enrichment with DIA-MS: a sensitive and selective approach for quantitative analysis of HCP.

Authors: Gong, Yachuan; Zhao, Yanjia; Peng, Rui; Qi, Wen; Zhang, Yi; Peng, Ying; Zheng, Jiang

Year: 2026

Venue: Talanta

DOI: https://doi.org/10.1016/j.talanta.2025.129052

Paper Sensitive methods for evaluation of antibodies for host cell protein analysis and screening of impurities in a vaccine process.

Lundström, Christine Sund; Mattsson, Anna; Lövgren, Karin et al. · 2014 · Vaccine · DOI

Full title: Sensitive methods for evaluation of antibodies for host cell protein analysis and screening of impurities in a vaccine process.

Authors: Lundström, Christine Sund; Mattsson, Anna; Lövgren, Karin; Eriksson, Åsa; Sundberg, Åsa Liljegren; Lundgren, Mats; Nilsson, Camilla Estmer

Year: 2014

Venue: Vaccine

DOI: https://doi.org/10.1016/j.vaccine.2014.02.032

Paper Degradation of Polysorbate 20 by Sialate O-Acetylesterase in Monoclonal Antibody Formulations.

Zhang, Sisi; Xiao, Hui; Li, Ning · 2021 · Journal of pharmaceutical sciences · DOI

Full title: Degradation of Polysorbate 20 by Sialate O-Acetylesterase in Monoclonal Antibody Formulations.

Authors: Zhang, Sisi; Xiao, Hui; Li, Ning

Year: 2021

Venue: Journal of pharmaceutical sciences

DOI: https://doi.org/10.1016/j.xphs.2021.09.001

Paper Identification and Quantification of a Problematic Host Cell Protein to Support Therapeutic Protein Development.

E, Sook Yen; Hu, Yunli; Molden, Rosalynn et al. · 2023 · Journal of pharmaceutical sciences · DOI

Full title: Identification and Quantification of a Problematic Host Cell Protein to Support Therapeutic Protein Development.

Authors: E, Sook Yen; Hu, Yunli; Molden, Rosalynn; Qiu, Haibo; Li, Ning

Year: 2023

Venue: Journal of pharmaceutical sciences

DOI: https://doi.org/10.1016/j.xphs.2022.10.008

Paper Polysorbate Degradation and Particle Formation in a High Concentration mAb: Formulation Strategies to Minimize Effect of Enzymatic Polysorbate Degradation.

Roy, Ian; Patel, Ashaben; Kumar, Vineet et al. · 2021 · Journal of pharmaceutical sciences · DOI

Full title: Polysorbate Degradation and Particle Formation in a High Concentration mAb: Formulation Strategies to Minimize Effect of Enzymatic Polysorbate Degradation.

Authors: Roy, Ian; Patel, Ashaben; Kumar, Vineet; Nanda, Tatiana; Assenberg, Rene; Wuchner, Klaus; Amin, Ketan

Year: 2021

Venue: Journal of pharmaceutical sciences

DOI: https://doi.org/10.1016/j.xphs.2021.05.012

Paper Identification and Characterization of Polysorbate-Degrading Enzymes in a Monoclonal Antibody Formulation.

Graf, Tobias; Tomlinson, Anthony; Yuk, Inn H et al. · 2021 · Journal of pharmaceutical sciences · DOI

Full title: Identification and Characterization of Polysorbate-Degrading Enzymes in a Monoclonal Antibody Formulation.

Authors: Graf, Tobias; Tomlinson, Anthony; Yuk, Inn H; Kufer, Regina; Spensberger, Bernhard; Falkenstein, Roberto; Shen, Amy; Li, Hong; Duan, Dana; Liu, Wenqiang; Wohlrab, Stefanie; Edelmann, Franziska; Leiss, Michael

Year: 2021

Venue: Journal of pharmaceutical sciences

DOI: https://doi.org/10.1016/j.xphs.2021.06.033

Paper Improved identification of host cell proteins in monoclonal antibodies by combining filter-aided sample preparation and native digestion.

Yang, Yang; Wu, Jing; Wang, Fang et al. · 2025 · Journal of pharmaceutical sciences · DOI

Full title: Improved identification of host cell proteins in monoclonal antibodies by combining filter-aided sample preparation and native digestion.

Authors: Yang, Yang; Wu, Jing; Wang, Fang; Lefers, Mark

Year: 2025

Venue: Journal of pharmaceutical sciences

DOI: https://doi.org/10.1016/j.xphs.2025.103875

Paper A workflow for accurate and consistent quantitation of host cell proteins by SWATH LC-MS/MS analysis to support process development.

Guo, Jia; Kufer, Regina; Greenwood-Goodwin, Midori et al. · 2025 · Journal of pharmaceutical sciences · DOI

Full title: A workflow for accurate and consistent quantitation of host cell proteins by SWATH LC-MS/MS analysis to support process development.

Authors: Guo, Jia; Kufer, Regina; Greenwood-Goodwin, Midori; Wohlrab, Stefanie; Woodruff, Fem; Li, Delia; Reckermann, Katharina; Youn, Jonghae; Kandula, Dilip Kumar Reddy; Xiong, Lei; Yang, Feng

Year: 2025

Venue: Journal of pharmaceutical sciences

DOI: https://doi.org/10.1016/j.xphs.2024.11.007

Paper Characterization of Recombinantly-Expressed Hydrolytic Enzymes from Chinese Hamster Ovary Cells: Identification of Host Cell Proteins that Degrade Polysorbate.

Kovner, Daniel; Yuk, Inn H; Shen, Amy et al. · 2023 · Journal of pharmaceutical sciences · DOI

Full title: Characterization of Recombinantly-Expressed Hydrolytic Enzymes from Chinese Hamster Ovary Cells: Identification of Host Cell Proteins that Degrade Polysorbate.

Authors: Kovner, Daniel; Yuk, Inn H; Shen, Amy; Li, Hong; Graf, Tobias; Gupta, Sanjay; Liu, Wenqiang; Tomlinson, Anthony

Year: 2023

Venue: Journal of pharmaceutical sciences

DOI: https://doi.org/10.1016/j.xphs.2023.01.003

Paper A Highly Sensitive LC-MS/MS Method for Targeted Quantitation of Lipase Host Cell Proteins in Biotherapeutics.

Chen, Yunqiu; Xu, Chong-Feng; Stanley, Bradley et al. · 2021 · Journal of pharmaceutical sciences · DOI

Full title: A Highly Sensitive LC-MS/MS Method for Targeted Quantitation of Lipase Host Cell Proteins in Biotherapeutics.

Authors: Chen, Yunqiu; Xu, Chong-Feng; Stanley, Bradley; Evangelist, Greg; Brinkmann, Alex; Liu, Suli; McCarthy, Sean; Xiong, Lei; Jones, Elliott; Sosic, Zoran; Yeung, Bernice

Year: 2021

Venue: Journal of pharmaceutical sciences

DOI: https://doi.org/10.1016/j.xphs.2021.08.024

Paper Host cell protein detection via surfactant-assisted acid precipitation.

Tomono, Haruna; Su, Yue; Adaniya, Stephanie et al. · 2025 · Journal of pharmaceutical sciences · DOI

Full title: Host cell protein detection via surfactant-assisted acid precipitation.

Authors: Tomono, Haruna; Su, Yue; Adaniya, Stephanie; Zhao, Bo; Xiao, Hui; Li, Ning

Year: 2025

Venue: Journal of pharmaceutical sciences

DOI: https://doi.org/10.1016/j.xphs.2025.104008

Paper Investigating pH Effects on Enzymes Catalyzing Polysorbate Degradation by Activity-Based Protein Profiling.

Dehghani, Alireza; Binder, Florian; Zorn, Michael et al. · 2024 · Journal of pharmaceutical sciences · DOI

Full title: Investigating pH Effects on Enzymes Catalyzing Polysorbate Degradation by Activity-Based Protein Profiling.

Authors: Dehghani, Alireza; Binder, Florian; Zorn, Michael; Feigler, Andreas; Fischer, Kathrin Inge; Felix, Marius Nicolaus; Happersberger, Peter; Reisinger, Bernd

Year: 2024

Venue: Journal of pharmaceutical sciences

DOI: https://doi.org/10.1016/j.xphs.2023.09.013

Paper Identification of Acyl-Protein Thioesterase-1 as a Polysorbate-Degrading Host Cell Protein in a Monoclonal Antibody Formulation Using Activity-Based Protein Profiling.

Šprager, Ernest; Möller, Jens; Lin, Yuhsien et al. · 2024 · Journal of pharmaceutical sciences · DOI

Full title: Identification of Acyl-Protein Thioesterase-1 as a Polysorbate-Degrading Host Cell Protein in a Monoclonal Antibody Formulation Using Activity-Based Protein Profiling.

Authors: Šprager, Ernest; Möller, Jens; Lin, Yuhsien; Reisinger, Veronika; Bratkovič, Tomaž; Lunder, Mojca; Vašl, Jožica; Krajnc, Aleksander

Year: 2024

Venue: Journal of pharmaceutical sciences

DOI: https://doi.org/10.1016/j.xphs.2024.05.013

Paper Elucidating uptake and metabolic fate of dipeptides in CHO cell cultures using 13C labeling experiments and kinetic modeling.

Harnish Mukesh Naik; Xiangchen Cai; Pranay Ladiwala et al. · 2024 · Metabolic Engineering · DOI

Full title: Elucidating uptake and metabolic fate of dipeptides in CHO cell cultures using 13C labeling experiments and kinetic modeling.

Authors: Harnish Mukesh Naik; Xiangchen Cai; Pranay Ladiwala; J. Reddy; M. Betenbaugh; M. Antoniewicz

Year: 2024

Venue: Metabolic Engineering

DOI: https://doi.org/10.1016/j.ymben.2024.03.002

Paper Development of a quantitative assay for residual host cell proteins in a recombinant subunit vaccine against human respiratory syncytial virus.

Dagouassat, N; Haeuw, J F; Robillard, V et al. · 2001 · Journal of immunological methods · DOI

Full title: Development of a quantitative assay for residual host cell proteins in a recombinant subunit vaccine against human respiratory syncytial virus.

Authors: Dagouassat, N; Haeuw, J F; Robillard, V; Damien, F; Libon, C; Corvaïa, N; Lawny, F; Nguyen, T N; Bonnefoy, J Y; Beck, A

Year: 2001

Venue: Journal of immunological methods

DOI: https://doi.org/10.1016/s0022-1759(01)00321-0

Paper Fast, Robust, and Sensitive Identification of Residual Host Cell Proteins in Recombinant Monoclonal Antibodies Using Sodium Deoxycholate Assisted Digestion.

Li, Delia; Farchone, Adam; Zhu, Qing et al. · 2020 · Analytical chemistry · DOI

Full title: Fast, Robust, and Sensitive Identification of Residual Host Cell Proteins in Recombinant Monoclonal Antibodies Using Sodium Deoxycholate Assisted Digestion.

Authors: Li, Delia; Farchone, Adam; Zhu, Qing; Macchi, Frank; Walker, Donald E; Michels, David A; Yang, Feng

Year: 2020

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.0c02258

Paper Ultrasensitive Quantification Method for Understanding Biologically Relevant Concentrations of Host Cell Proteins in Therapeutics.

Zhang, Sisi; Zhao, Bo; Adaniya, Stephanie et al. · 2023 · Analytical chemistry · DOI

Full title: Ultrasensitive Quantification Method for Understanding Biologically Relevant Concentrations of Host Cell Proteins in Therapeutics.

Authors: Zhang, Sisi; Zhao, Bo; Adaniya, Stephanie; Xiao, Hui; Li, Ning

Year: 2023

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.3c00020

Paper Detection of contaminating enzymatic activity in plant-derived recombinant biotechnology products.

Brinson, Robert G; Giulian, Gary G; Kelman, Zvi et al. · 2014 · Analytical chemistry · DOI

Full title: Detection of contaminating enzymatic activity in plant-derived recombinant biotechnology products.

Authors: Brinson, Robert G; Giulian, Gary G; Kelman, Zvi; Marino, John P

Year: 2014

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/ac503864m

Paper A Novel Sample Preparation for Shotgun Proteomics Characterization of HCPs in Antibodies.

Huang, Lihua; Wang, Ning; Mitchell, Charles E et al. · 2017 · Analytical chemistry · DOI

Full title: A Novel Sample Preparation for Shotgun Proteomics Characterization of HCPs in Antibodies.

Authors: Huang, Lihua; Wang, Ning; Mitchell, Charles E; Brownlee, Tammy; Maple, Steven R; De Felippis, Michael R

Year: 2017

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.7b00304

Paper Enhancing Host-Cell Protein Detection in Protein Therapeutics Using HILIC Enrichment and Proteomic Analysis.

Wang, Qingyi; Slaney, Thomas R; Wu, Wei et al. · 2020 · Analytical chemistry · DOI

Full title: Enhancing Host-Cell Protein Detection in Protein Therapeutics Using HILIC Enrichment and Proteomic Analysis.

Authors: Wang, Qingyi; Slaney, Thomas R; Wu, Wei; Ludwig, Richard; Tao, Li; Leone, Anthony

Year: 2020

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.0c00360

Paper Enhanced Detection of Low-Abundance Host Cell Protein Impurities in High-Purity Monoclonal Antibodies Down to 1 ppm Using Ion Mobility Mass Spectrometry Coupled with Multidimensional Liquid Chromatography.

Doneanu, Catalin E; Anderson, Malcolm; Williams, Brad J et al. · 2015 · Analytical chemistry · DOI

Full title: Enhanced Detection of Low-Abundance Host Cell Protein Impurities in High-Purity Monoclonal Antibodies Down to 1 ppm Using Ion Mobility Mass Spectrometry Coupled with Multidimensional Liquid Chromatography.

Authors: Doneanu, Catalin E; Anderson, Malcolm; Williams, Brad J; Lauber, Matthew A; Chakraborty, Asish; Chen, Weibin

Year: 2015

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.5b02103

Paper Host Cell Protein Profiling by Targeted and Untargeted Analysis of Data Independent Acquisition Mass Spectrometry Data with Parallel Reaction Monitoring Verification.

Kreimer, Simion; Gao, Yuanwei; Ray, Somak et al. · 2017 · Analytical chemistry · DOI

Full title: Host Cell Protein Profiling by Targeted and Untargeted Analysis of Data Independent Acquisition Mass Spectrometry Data with Parallel Reaction Monitoring Verification.

Authors: Kreimer, Simion; Gao, Yuanwei; Ray, Somak; Jin, Mi; Tan, Zhijun; Mussa, Nesredin A; Tao, Li; Li, Zhengjian; Ivanov, Alexander R; Karger, Barry L

Year: 2017

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.6b04892

Paper Simple and Sensitive Method for Deep Profiling of Host Cell Proteins in Therapeutic Antibodies by Combining Ultra-Low Trypsin Concentration Digestion, Long Chromatographic Gradients, and BoxCar Mass Spectrometry Acquisition.

Nie, Song; Greer, Tyler; O'Brien Johnson, Reid et al. · 2021 · Analytical chemistry · DOI

Full title: Simple and Sensitive Method for Deep Profiling of Host Cell Proteins in Therapeutic Antibodies by Combining Ultra-Low Trypsin Concentration Digestion, Long Chromatographic Gradients, and BoxCar Mass Spectrometry Acquisition.

Authors: Nie, Song; Greer, Tyler; O'Brien Johnson, Reid; Zheng, Xiaojing; Torri, Albert; Li, Ning

Year: 2021

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.0c03931

Paper Combination of FAIMS, Protein A Depletion, and Native Digest Conditions Enables Deep Proteomic Profiling of Host Cell Proteins in Monoclonal Antibodies.

Johnson, Reid O'Brien; Greer, Tyler; Cejkov, Milos et al. · 2020 · Analytical chemistry · DOI

Full title: Combination of FAIMS, Protein A Depletion, and Native Digest Conditions Enables Deep Proteomic Profiling of Host Cell Proteins in Monoclonal Antibodies.

Authors: Johnson, Reid O'Brien; Greer, Tyler; Cejkov, Milos; Zheng, Xiaojing; Li, Ning

Year: 2020

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.0c01175

Paper Standard-Free Absolute Quantitation of Antibody Deamidation Degradation and Host Cell Proteins by Coulometric Mass Spectrometry

Ai, Yongling; Gunawardena, Harsha P.; Li, Xuanwen et al. · 2022 · Analytical Chemistry · DOI

Full title: Standard-Free Absolute Quantitation of Antibody Deamidation Degradation and Host Cell Proteins by Coulometric Mass Spectrometry

Authors: Ai, Yongling; Gunawardena, Harsha P.; Li, Xuanwen; Kim, Yong-Ick; Dewald, Howard D.; Chen, Hao

Year: 2022

Venue: Analytical Chemistry

DOI: https://doi.org/10.1021/acs.analchem.2c02709

Paper Versatile LC-MS-Based Workflow with Robust 0.1 ppm Sensitivity for Identifying Residual HCPs in Biotherapeutic Products.

Yang, Feng; Li, Delia; Kufer, Regina et al. · 2022 · Analytical chemistry · DOI

Full title: Versatile LC-MS-Based Workflow with Robust 0.1 ppm Sensitivity for Identifying Residual HCPs in Biotherapeutic Products.

Authors: Yang, Feng; Li, Delia; Kufer, Regina; Cadang, Lance; Zhang, Jennifer; Dai, Lu; Guo, Jia; Wohlrab, Stefanie; Greenwood-Goodwin, Midori; Shen, Amy; Duan, Dana; Li, Hong; Yuk, Inn H

Year: 2022

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.1c03095

Paper Native Digestion and Shotgun Proteomics for Host Cell Protein Profiling of Adeno-Associated Viruses.

Lodge, Jean M; Huang, Lihua; Lian, Zhirui et al. · 2024 · Analytical chemistry · DOI

Full title: Native Digestion and Shotgun Proteomics for Host Cell Protein Profiling of Adeno-Associated Viruses.

Authors: Lodge, Jean M; Huang, Lihua; Lian, Zhirui; Qian, Jun; Tian, Yuwei

Year: 2024

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.4c00893

Paper A 2D LC-MS/MS Strategy for Reliable Detection of 10-ppm Level Residual Host Cell Proteins in Therapeutic Antibodies.

Yang, Feng; Walker, Donald E; Schoenfelder, Jeannine et al. · 2018 · Analytical chemistry · DOI

Full title: A 2D LC-MS/MS Strategy for Reliable Detection of 10-ppm Level Residual Host Cell Proteins in Therapeutic Antibodies.

Authors: Yang, Feng; Walker, Donald E; Schoenfelder, Jeannine; Carver, Joseph; Zhang, Alice; Li, Delia; Harris, Reed; Stults, John T; Yu, X Christopher; Michels, David A

Year: 2018

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.8b03044

Paper Quantitative host cell protein analysis using two dimensional data independent LC-MS(E).

Farrell, Amy; Mittermayr, Stefan; Morrissey, Brian et al. · 2015 · Analytical chemistry · DOI

Full title: Quantitative host cell protein analysis using two dimensional data independent LC-MS(E).

Authors: Farrell, Amy; Mittermayr, Stefan; Morrissey, Brian; Mc Loughlin, Niaobh; Navas Iglesias, Natalia; Marison, Ian W; Bones, Jonathan

Year: 2015

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.5b01377

Paper diaPASEF Enables High-Throughput Proteomic Analysis of Host Cell Proteins for Biopharmaceutical Process Development.

Tsukidate, Taku; Stiving, Alyssa Q; Rivera, Shannon et al. · 2024 · Analytical chemistry · DOI

Full title: diaPASEF Enables High-Throughput Proteomic Analysis of Host Cell Proteins for Biopharmaceutical Process Development.

Authors: Tsukidate, Taku; Stiving, Alyssa Q; Rivera, Shannon; Sahoo, Ansuman; Madabhushi, Sri; Li, Xuanwen

Year: 2024

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.4c00977

Paper Host Cell Protein Profiling in Biopharmaceutical Harvests

Darja, Obrstar; Kröner, Frieder; Japelj, Boštjan et al. · 2018 · Analytical Chemistry · DOI

Full title: Host Cell Protein Profiling in Biopharmaceutical Harvests

Authors: Darja, Obrstar; Kröner, Frieder; Japelj, Boštjan; Bojic, Lea; Anderka, Oliver

Year: 2018

Venue: Analytical Chemistry

DOI: https://doi.org/10.1021/acs.analchem.8b01236

Paper Targeted Host Cell Protein Quantification by LC-MRM Enables Biologics Processing and Product Characterization.

Gao, Xinliu; Rawal, Baibhav; Wang, Yi et al. · 2020 · Analytical chemistry · DOI

Full title: Targeted Host Cell Protein Quantification by LC-MRM Enables Biologics Processing and Product Characterization.

Authors: Gao, Xinliu; Rawal, Baibhav; Wang, Yi; Li, Xuanwen; Wylie, David; Liu, Yan-Hui; Breunig, Lloyd; Driscoll, Dennis; Wang, Fengqiang; Richardson, Douglas D

Year: 2020

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.9b03952

Paper Strategy for Generating Sequence-Defined Aptamer Reagent Sets for Detecting Protein Contaminants in Biotherapeutics.

McGivney, James B; Csordas, Andrew T; Walker, Faye M et al. · 2018 · Analytical chemistry · DOI

Full title: Strategy for Generating Sequence-Defined Aptamer Reagent Sets for Detecting Protein Contaminants in Biotherapeutics.

Authors: McGivney, James B; Csordas, Andrew T; Walker, Faye M; Bagley, Elizabeth R; Gruber, Emily M; Mage, Peter L; Casas-Finet, Jose; Nakamoto, Margaret A; Eisenstein, Michael; Larkin, Christopher J; Strouse, Robert J; Soh, H Tom

Year: 2018

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.7b04775

Paper Dual Data-Independent Acquisition Approach Combining Global HCP Profiling and Absolute Quantification of Key Impurities during Bioprocess Development.

Husson, Gauthier; Delangle, Aurélie; O'Hara, John et al. · 2018 · Analytical chemistry · DOI

Full title: Dual Data-Independent Acquisition Approach Combining Global HCP Profiling and Absolute Quantification of Key Impurities during Bioprocess Development.

Authors: Husson, Gauthier; Delangle, Aurélie; O'Hara, John; Cianferani, Sarah; Gervais, Annick; Van Dorsselaer, Alain; Bracewell, Dan; Carapito, Christine

Year: 2018

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.7b03965

Paper Sensitive, Rapid, Robust, and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Development.

Ma, Jiao; Kilby, Greg W · 2020 · Journal of proteome research · DOI

Full title: Sensitive, Rapid, Robust, and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Development.

Authors: Ma, Jiao; Kilby, Greg W

Year: 2020

Venue: Journal of proteome research

DOI: https://doi.org/10.1021/acs.jproteome.0c00252

Paper Evaluation of Peptide Fractionation and Native Digestion as Two Novel Sample Preparation Workflows to Improve HCP Characterization by LC-MS/MS.

Kufer, Regina; Haindl, Markus; Wegele, Harald et al. · 2019 · Analytical chemistry · DOI

Full title: Evaluation of Peptide Fractionation and Native Digestion as Two Novel Sample Preparation Workflows to Improve HCP Characterization by LC-MS/MS.

Authors: Kufer, Regina; Haindl, Markus; Wegele, Harald; Wohlrab, Stefanie

Year: 2019

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.9b01259

Paper Optimized Sample Preparation and Data Processing of Data-Independent Acquisition Methods for the Robust Quantification of Trace-Level Host Cell Protein Impurities in Antibody Drug Products.

Pythoud, Nicolas; Bons, Joanna; Mijola, Geoffroy et al. · 2021 · Journal of proteome research · DOI

Full title: Optimized Sample Preparation and Data Processing of Data-Independent Acquisition Methods for the Robust Quantification of Trace-Level Host Cell Protein Impurities in Antibody Drug Products.

Authors: Pythoud, Nicolas; Bons, Joanna; Mijola, Geoffroy; Beck, Alain; Cianférani, Sarah; Carapito, Christine

Year: 2021

Venue: Journal of proteome research

DOI: https://doi.org/10.1021/acs.jproteome.0c00664

Paper Proteomics Strategies for Mass Spectrometry Analysis of Host Cell Proteins in Monoclonal Antibody Bioprocessing.

Cao, Huiting; Xiong, Jingyuan; Su, Yang et al. · 2026 · Journal of proteome research · DOI

Full title: Proteomics Strategies for Mass Spectrometry Analysis of Host Cell Proteins in Monoclonal Antibody Bioprocessing.

Authors: Cao, Huiting; Xiong, Jingyuan; Su, Yang; Liu, Xiuxiu; Tang, Tao; Tang, Yao; Yu, Wen; Li, Yan; Li, Dapeng

Year: 2026

Venue: Journal of proteome research

DOI: https://doi.org/10.1021/acs.jproteome.5c01041

Paper Development of an Optimized LC-MS Workflow for Host Cell Protein Characterization to Support Upstream Process Development.

Seidel, Janik D; Condina, Mark R; Klingler-Hoffmann, Manuela et al. · 2025 · Journal of proteome research · DOI

Full title: Development of an Optimized LC-MS Workflow for Host Cell Protein Characterization to Support Upstream Process Development.

Authors: Seidel, Janik D; Condina, Mark R; Klingler-Hoffmann, Manuela; Young, Clifford; Donnellan, Leigh; Kyngdon, Craig; Hoffmann, Peter

Year: 2025

Venue: Journal of proteome research

DOI: https://doi.org/10.1021/acs.jproteome.4c00637

Paper Elucidation of the CHO Super-Ome (CHO-SO) by Proteoinformatics.

Kumar, Amit; Baycin-Hizal, Deniz; Wolozny, Daniel et al. · 2015 · Journal of proteome research · DOI

Full title: Elucidation of the CHO Super-Ome (CHO-SO) by Proteoinformatics.

Authors: Kumar, Amit; Baycin-Hizal, Deniz; Wolozny, Daniel; Pedersen, Lasse Ebdrup; Lewis, Nathan E; Heffner, Kelley; Chaerkady, Raghothama; Cole, Robert N; Shiloach, Joseph; Zhang, Hui; Bowen, Michael A; Betenbaugh, Michael J

Year: 2015

Venue: Journal of proteome research

DOI: https://doi.org/10.1021/acs.jproteome.5b00588

Paper Carrier-Guided Proteome Analysis in a High Protein Background: An Improved Approach to Host Cell Protein Identification.

Karmani, Divyanshi; Seifihesar, Niloofar; Sultonova, Mukhayyo et al. · 2024 · Journal of proteome research · DOI

Full title: Carrier-Guided Proteome Analysis in a High Protein Background: An Improved Approach to Host Cell Protein Identification.

Authors: Karmani, Divyanshi; Seifihesar, Niloofar; Sultonova, Mukhayyo; Blackmore, Beau; Paulo, Joao A; Harty, Matthew; Murphy, J Patrick

Year: 2024

Venue: Journal of proteome research

DOI: https://doi.org/10.1021/acs.jproteome.4c00158

Paper Chromatographic Analysis of Host-Cell Protein Impurities in Pharmaceuticals Derived from Recombinant DNA

O'Keefe, Donald O.; Will, Mark L. · 1992 · ACS symposium series · DOI

Full title: Chromatographic Analysis of Host-Cell Protein Impurities in Pharmaceuticals Derived from Recombinant DNA

Authors: O'Keefe, Donald O.; Will, Mark L.

Year: 1992

Venue: ACS symposium series

DOI: https://doi.org/10.1021/bk-1992-0512.ch010

Paper Improved Identification and Quantification of Host Cell Proteins (HCPs) in Biotherapeutics Using Liquid Chromatography-Mass Spectrometry

Chen, Weibin; Doneanu, Catalin E.; Lauber, Matthew et al. · 2015 · ACS symposium series · DOI

Full title: Improved Identification and Quantification of Host Cell Proteins (HCPs) in Biotherapeutics Using Liquid Chromatography-Mass Spectrometry

Authors: Chen, Weibin; Doneanu, Catalin E.; Lauber, Matthew; Koza, Stephan M.; Prakash, Kesh; Stapels, Martha; Fountain, Kenneth J.

Year: 2015

Venue: ACS symposium series

DOI: https://doi.org/10.1021/bk-2015-1202.ch013

Paper A Novel Strategy to Rapidly Profile and Quantify High-Risk Host Cell Proteins Using Integrated DDA-PRM-dMRM Mode.

Xu, Xuan; Wang, Lan; Wu, Gang et al. · 2025 · Journal of proteome research · DOI

Full title: A Novel Strategy to Rapidly Profile and Quantify High-Risk Host Cell Proteins Using Integrated DDA-PRM-dMRM Mode.

Authors: Xu, Xuan; Wang, Lan; Wu, Gang; Xu, Shengyuan; Li, Yang; Sheng, Ning; Zhang, Jinlan

Year: 2025

Venue: Journal of proteome research

DOI: https://doi.org/10.1021/acs.jproteome.5c00369

Paper High-Risk Host Cell Protein Profiling with Sub-ppm Sensitivity by iRT-Assisted Targeted Mass Spectrometry.

Wang, Qi; Dong, Ting; Li, Muchen et al. · 2026 · Journal of proteome research · DOI

Full title: High-Risk Host Cell Protein Profiling with Sub-ppm Sensitivity by iRT-Assisted Targeted Mass Spectrometry.

Authors: Wang, Qi; Dong, Ting; Li, Muchen; Wang, Xueliang; Xu, Min; Cui, Yiqing; Zhu, He; Zhang, Tianli; Gao, Xingli; Zhu, Lin; Wang, Lili; Yu, Le; Xiao, Yongsheng; Tian, Jun

Year: 2026

Venue: Journal of proteome research

DOI: https://doi.org/10.1021/acs.jproteome.5c00680

Paper Optimized removal of soluble host cell proteins for the recovery of met-human growth hormone inclusion bodies from Escherichia coli cell lysate using crossflow microfiltration.

Venkiteshwaran, Adith; Heider, Patrick; Matosevic, Sandro et al. · 2007 · Biotechnology progress · DOI

Full title: Optimized removal of soluble host cell proteins for the recovery of met-human growth hormone inclusion bodies from Escherichia coli cell lysate using crossflow microfiltration.

Authors: Venkiteshwaran, Adith; Heider, Patrick; Matosevic, Sandro; Bogsnes, Are; Staby, Arne; Sharfstein, Susan; Belfort, Georges

Year: 2007

Venue: Biotechnology progress

DOI: https://doi.org/10.1021/bp0700253

Paper The Dual-Integrated Multi-Attribute Method: Simultaneous Evaluation of Post-Translational Modifications and Host Cell Proteins for Unpurified Bulk Harvest of Antibody Therapeutics.

Kawase, Naoki; Urasawa, Takaya; Hirakura, Yutaka et al. · 2026 · ACS omega · DOI

Full title: The Dual-Integrated Multi-Attribute Method: Simultaneous Evaluation of Post-Translational Modifications and Host Cell Proteins for Unpurified Bulk Harvest of Antibody Therapeutics.

Authors: Kawase, Naoki; Urasawa, Takaya; Hirakura, Yutaka; Kawasaki, Nana

Year: 2026

Venue: ACS omega

DOI: https://doi.org/10.1021/acsomega.5c11877

Paper Selective Leakage of Host-Cell Proteins during High-Cell-Density Cultivation of Recombinant and Non-recombinant Escherichia coli

Rinas, Ursula; Hoffmann, F. Michael · 2004 · Biotechnology Progress · DOI

Full title: Selective Leakage of Host-Cell Proteins during High-Cell-Density Cultivation of Recombinant and Non-recombinant Escherichia coli

Authors: Rinas, Ursula; Hoffmann, F. Michael

Year: 2004

Venue: Biotechnology Progress

DOI: https://doi.org/10.1021/bp034348k

Paper Demonstration of robust host cell protein clearance in biopharmaceutical downstream processes.

Shukla, Abhinav A; Jiang, Canping; Ma, Junfen et al. · 2008 · Biotechnology progress · DOI

Full title: Demonstration of robust host cell protein clearance in biopharmaceutical downstream processes.

Authors: Shukla, Abhinav A; Jiang, Canping; Ma, Junfen; Rubacha, Michael; Flansburg, Lisa; Lee, Steven S

Year: 2008

Venue: Biotechnology progress

DOI: https://doi.org/10.1021/bp070396j

Paper Systematic Optimization of Activity-Based Protein Profiling for Identification of Polysorbate-Degradative Enzymes in Biotherapeutic Drug Substance down to 10 ppb.

Tsukidate, Taku; Liu, Anita P; Rivera, Shannon et al. · 2024 · Journal of the American Society for Mass Spectrometry · DOI

Full title: Systematic Optimization of Activity-Based Protein Profiling for Identification of Polysorbate-Degradative Enzymes in Biotherapeutic Drug Substance down to 10 ppb.

Authors: Tsukidate, Taku; Liu, Anita P; Rivera, Shannon; Stiving, Alyssa Q; Welch, Jonathan; Li, Xuanwen

Year: 2024

Venue: Journal of the American Society for Mass Spectrometry

DOI: https://doi.org/10.1021/jasms.4c00387

Paper Tracking the Behavior of Monoclonal Antibody Product Quality Attributes Using a Multi-Attribute Method Workflow.

Jakes, Craig; Millán-Martín, Silvia; Carillo, Sara et al. · 2021 · Journal of the American Society for Mass Spectrometry · DOI

Full title: Tracking the Behavior of Monoclonal Antibody Product Quality Attributes Using a Multi-Attribute Method Workflow.

Authors: Jakes, Craig; Millán-Martín, Silvia; Carillo, Sara; Scheffler, Kai; Zaborowska, Izabela; Bones, Jonathan

Year: 2021

Venue: Journal of the American Society for Mass Spectrometry

DOI: https://doi.org/10.1021/jasms.0c00432

Paper Exploitation of the Adsorptive Properties of Depth Filters for Host Cell Protein Removal during Monoclonal Antibody Purification

Yigzaw, Yinges; Piper, Robert C.; Tran, Mai H. et al. · 2006 · Biotechnology Progress · DOI

Full title: Exploitation of the Adsorptive Properties of Depth Filters for Host Cell Protein Removal during Monoclonal Antibody Purification

Authors: Yigzaw, Yinges; Piper, Robert C.; Tran, Mai H.; Shukla, Abhinav A.

Year: 2006

Venue: Biotechnology Progress

DOI: https://doi.org/10.1021/bp050274w

Paper Identifying the CHO secretome using mucin-type O-linked glycosylation and click-chemistry.

Slade, Peter G; Hajivandi, Mahbod; Bartel, Cheryl Moody et al. · 2012 · Journal of proteome research · DOI

Full title: Identifying the CHO secretome using mucin-type O-linked glycosylation and click-chemistry.

Authors: Slade, Peter G; Hajivandi, Mahbod; Bartel, Cheryl Moody; Gorfien, Stephen F

Year: 2012

Venue: Journal of proteome research

DOI: https://doi.org/10.1021/pr300810f

Paper Expanded bed adsorption in the purification of monoclonal antibodies: a comparison of process alternatives.

Blank, G S; Zapata, G; Fahrner, R et al. · 2001 · Bioseparation · DOI

Full title: Expanded bed adsorption in the purification of monoclonal antibodies: a comparison of process alternatives.

Authors: Blank, G S; Zapata, G; Fahrner, R; Milton, M; Yedinak, C; Knudsen, H; Schmelzer, C

Year: 2001

Venue: Bioseparation

DOI: https://doi.org/10.1023/a:1012278022349

Paper A modular and adaptive mass spectrometry-based platform for support of bioprocess development toward optimal host cell protein clearance.

Walker, Donald E; Yang, Feng; Carver, Joseph et al. · 2017 · mAbs · DOI

Full title: A modular and adaptive mass spectrometry-based platform for support of bioprocess development toward optimal host cell protein clearance.

Authors: Walker, Donald E; Yang, Feng; Carver, Joseph; Joe, Koman; Michels, David A; Yu, X Christopher

Year: 2017

Venue: mAbs

DOI: https://doi.org/10.1080/19420862.2017.1303023

Paper Host cell protein profiling of commercial therapeutic protein drugs as a benchmark for monoclonal antibody-based therapeutic protein development.

Molden, Rosalynn; Hu, Mengqi; Yen E, Sook et al. · 2021 · mAbs · DOI

Full title: Host cell protein profiling of commercial therapeutic protein drugs as a benchmark for monoclonal antibody-based therapeutic protein development.

Authors: Molden, Rosalynn; Hu, Mengqi; Yen E, Sook; Saggese, Diana; Reilly, James; Mattila, John; Qiu, Haibo; Chen, Gang; Bak, Hanne; Li, Ning

Year: 2021

Venue: mAbs

DOI: https://doi.org/10.1080/19420862.2021.1955811

Paper Technical advancement and practical considerations of LC-MS/MS-based methods for host cell protein identification and quantitation to support process development.

Guo, Jia; Kufer, Regina; Li, Delia et al. · 2023 · mAbs · DOI

Full title: Technical advancement and practical considerations of LC-MS/MS-based methods for host cell protein identification and quantitation to support process development.

Authors: Guo, Jia; Kufer, Regina; Li, Delia; Wohlrab, Stefanie; Greenwood-Goodwin, Midori; Yang, Feng

Year: 2023

Venue: mAbs

DOI: https://doi.org/10.1080/19420862.2023.2213365

Paper ELISA reagent coverage evaluation by affinity purification tandem mass spectrometry.

Henry, Scott M; Sutlief, Elissa; Salas-Solano, Oscar et al. · 2017 · mAbs · DOI

Full title: ELISA reagent coverage evaluation by affinity purification tandem mass spectrometry.

Authors: Henry, Scott M; Sutlief, Elissa; Salas-Solano, Oscar; Valliere-Douglass, John

Year: 2017

Venue: mAbs

DOI: https://doi.org/10.1080/19420862.2017.1349586

Paper Insights into ultra-low affinity lipase-antibody noncovalent complex binding mechanisms.

Hecht, Elizabeth Sara; Mehta, Shrenik; Wecksler, Aaron T et al. · 2022 · mAbs · DOI

Full title: Insights into ultra-low affinity lipase-antibody noncovalent complex binding mechanisms.

Authors: Hecht, Elizabeth Sara; Mehta, Shrenik; Wecksler, Aaron T; Aguilar, Ben; Swanson, Nathaniel; Phung, Wilson; Dubey Kelsoe, Ananya; Benner, W Henry; Tesar, Devin; Kelley, Robert F; Sandoval, Wendy; Sreedhara, Alavattam

Year: 2022

Venue: mAbs

DOI: https://doi.org/10.1080/19420862.2022.2135183

Paper Host Cell Proteins Can Be Eliminated More Strategically

G. Macdonald · 2022 · Genetic Engineering & Biotechnology News · DOI

Full title: Host Cell Proteins Can Be Eliminated More Strategically

Authors: G. Macdonald

Year: 2022

Venue: Genetic Engineering & Biotechnology News

DOI: https://doi.org/10.1089/gen.42.09.13

Paper Illuminating a biologics development challenge: systematic characterization of CHO cell-derived hydrolases identified in monoclonal antibody formulations.

Maier, Melanie; Weiß, Linus; Zeh, Nikolas et al. · 2024 · mAbs · DOI

Full title: Illuminating a biologics development challenge: systematic characterization of CHO cell-derived hydrolases identified in monoclonal antibody formulations.

Authors: Maier, Melanie; Weiß, Linus; Zeh, Nikolas; Schmieder-Todtenhaupt, Valerie; Dehghani, Alireza; Felix, Marius Nicolaus; Heinzelmann, Daniel; Lindner, Benjamin; Schmidt, Moritz; Studts, Joey; Schulz, Patrick; Reisinger, Bernd; Otte, Kerstin; Franzreb, Matthias; Lakatos, Daniel; Fischer, Simon

Year: 2024

Venue: mAbs

DOI: https://doi.org/10.1080/19420862.2024.2375798

Paper Hamster neogenin, a host-cell protein contained in a respiratory syncytial virus candidate vaccine, induces antibody responses in rabbits but not in clinical trial participants

Steff, Ann‐Muriel; Cadieux-Dion, Chanel; Lannoy, Gaël de et al. · 2020 · Human Vaccines & Immunotherapeutics · DOI

Full title: Hamster neogenin, a host-cell protein contained in a respiratory syncytial virus candidate vaccine, induces antibody responses in rabbits but not in clinical trial participants

Authors: Steff, Ann‐Muriel; Cadieux-Dion, Chanel; Lannoy, Gaël de; Prato, Maria Key; Czeszak, Xavier; André, Bruno; Ingels, Dominique; Louckx, Marc; Dewé, Walthère; Picciolato, Marta; Maleux, Koen; Fissette, Laurence; Dieussaert, Ilse

Year: 2020

Venue: Human Vaccines & Immunotherapeutics

DOI: https://doi.org/10.1080/21645515.2019.1693749

Paper Mild hypothermic culture conditions affect residual host cell protein composition post-Protein A chromatography.

Goey, Cher Hui; Bell, David; Kontoravdi, Cleo · 2018 · mAbs · DOI

Full title: Mild hypothermic culture conditions affect residual host cell protein composition post-Protein A chromatography.

Authors: Goey, Cher Hui; Bell, David; Kontoravdi, Cleo

Year: 2018

Venue: mAbs

DOI: https://doi.org/10.1080/19420862.2018.1433977

Paper Host cell protein detection gap risk mitigation: quantitative IAC-MS for ELISA antibody reagent coverage determination.

Waldera-Lupa, Daniel M; Jasper, Yvonne; Köhne, Pia et al. · 2021 · mAbs · DOI

Full title: Host cell protein detection gap risk mitigation: quantitative IAC-MS for ELISA antibody reagent coverage determination.

Authors: Waldera-Lupa, Daniel M; Jasper, Yvonne; Köhne, Pia; Schwichtenhövel, Ronja; Falkenberg, Heiner; Flad, Thomas; Happersberger, Peter; Reisinger, Bernd; Dehghani, Alireza; Moussa, Roland; Waerner, Thomas

Year: 2021

Venue: mAbs

DOI: https://doi.org/10.1080/19420862.2021.1955432

Paper Performance comparison of Protein A affinity‐chromatography sorbents for purifying recombinant monoclonal antibodies

Fahrner, Robert; Whitney, Duncan; Vanderlaan, Martin et al. · 1999 · Biotechnology and Applied Biochemistry · DOI

Full title: Performance comparison of Protein A affinity‐chromatography sorbents for purifying recombinant monoclonal antibodies

Authors: Fahrner, Robert; Whitney, Duncan; Vanderlaan, Martin; Blank, Gregory S.

Year: 1999

Venue: Biotechnology and Applied Biochemistry

DOI: https://doi.org/10.1111/j.1470-8744.1999.tb00902.x

Paper Ceramic hydroxyapatite chromatography plays a critical role in bispecific antibody purification process for impurity removal.

Waller, Jessica A; Zheng, Ji; Dyer, Rachel et al. · 2023 · Antibody therapeutics · DOI

Full title: Ceramic hydroxyapatite chromatography plays a critical role in bispecific antibody purification process for impurity removal.

Authors: Waller, Jessica A; Zheng, Ji; Dyer, Rachel; Slaney, Thomas; Wu, Wei; Tao, Li; Ghose, Sanchayita

Year: 2023

Venue: Antibody therapeutics

DOI: https://doi.org/10.1093/abt/tbac030

Paper Evaluating Immunogenicity Risk Due to Host Cell Protein Impurities in Antibody-Based Biotherapeutics.

Jawa, Vibha; Joubert, Marisa K; Zhang, Qingchun et al. · 2016 · The AAPS journal · DOI

Full title: Evaluating Immunogenicity Risk Due to Host Cell Protein Impurities in Antibody-Based Biotherapeutics.

Authors: Jawa, Vibha; Joubert, Marisa K; Zhang, Qingchun; Deshpande, Meghana; Hapuarachchi, Suminda; Hall, Michael P; Flynn, Gregory C

Year: 2016

Venue: The AAPS journal

DOI: https://doi.org/10.1208/s12248-016-9948-4

Paper Improving Chinese hamster ovary host cell protein ELISA using Ella®: an automated microfluidic platform.

Van Manen-Brush, Kathleen; Zeitler, Jacob; White, John R et al. · 2020 · BioTechniques · DOI

Full title: Improving Chinese hamster ovary host cell protein ELISA using Ella®: an automated microfluidic platform.

Authors: Van Manen-Brush, Kathleen; Zeitler, Jacob; White, John R; Younge, Paul; Willis, Samantha; Jones, Marisa

Year: 2020

Venue: BioTechniques

DOI: https://doi.org/10.2144/btn-2020-0074

Paper Effect of Host Cell Protein on Chinese Hamster Ovary RecombinantProtein Production and its Removal Strategies: A Mini Review

Zhao, Yaru; He, Li; Fan, Zhenlin et al. · 2023 · Current Pharmaceutical Biotechnology · DOI

Full title: Effect of Host Cell Protein on Chinese Hamster Ovary RecombinantProtein Production and its Removal Strategies: A Mini Review

Authors: Zhao, Yaru; He, Li; Fan, Zhenlin; Wang, Tianyun

Year: 2023

Venue: Current Pharmaceutical Biotechnology

DOI: https://doi.org/10.2174/1389201024666230818112633

Paper Impact of next-generation high productivity perfusion cell culture process on host cell protein profile and a comparison with fed-batch cultures.

Hong, Jiyoung; Aswath, Minni; Gao, Yuan et al. · 2020 · DOI

Full title: Impact of next-generation high productivity perfusion cell culture process on host cell protein profile and a comparison with fed-batch cultures.

Authors: Hong, Jiyoung; Aswath, Minni; Gao, Yuan; Ogawa, Daisie; Sharma, Vaishali; Zhu, Min

Year: 2020

DOI: https://doi.org/10.22541/au.160552096.67354049/v1

Paper Development of a One-step Magnetic Separation-based Fluorescent Sandwich Assay for Residual Protein A in Antibody Drugs

Jie Xiong; Xuejun Yang; Jingxin Mao · 2025 · Current Analytical Chemistry · DOI

Full title: Development of a One-step Magnetic Separation-based Fluorescent Sandwich Assay for Residual Protein A in Antibody Drugs

Authors: Jie Xiong; Xuejun Yang; Jingxin Mao

Year: 2025

Venue: Current Analytical Chemistry

DOI: https://doi.org/10.2174/0115734110402682250820071640

Paper INCREASED THE SPECIFICITY AND SENSITIVITY OF MONOSPECIFIC ANTIBODY AGAINST HOST CELL PROTEIN (HCP) IN QUALITY CONTROL OF HEPATITIS B RECOMBINANT VACCINE

Marzieh, Hosseini Seyedeh; Bahman, Tabaraei; Shahin, Hadadian et al. · 2011 · Journal of paramedical sciences. · DOI

Full title: INCREASED THE SPECIFICITY AND SENSITIVITY OF MONOSPECIFIC ANTIBODY AGAINST HOST CELL PROTEIN (HCP) IN QUALITY CONTROL OF HEPATITIS B RECOMBINANT VACCINE

Authors: Marzieh, Hosseini Seyedeh; Bahman, Tabaraei; Shahin, Hadadian; Yavari, Fereshteh; Kamali-Jamil, Razieh; Shayestehpour, Mohammad; Mahdi, Nejati; Vaez, Javad

Year: 2011

Venue: Journal of paramedical sciences.

DOI: https://doi.org/10.22037/jps.v2i3.2539

Paper Cascading effects in bioprocessing: the impact of cell culture environment on CHO cell behaviour and host cell protein species

Goey, Cher Hui · 2016 · Spiral (Imperial College London) · DOI

Full title: Cascading effects in bioprocessing: the impact of cell culture environment on CHO cell behaviour and host cell protein species

Authors: Goey, Cher Hui

Year: 2016

Venue: Spiral (Imperial College London)

DOI: https://doi.org/10.25560/52703

Paper Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

Dickerson, Matthew T.; Leong, Kristen; Sheldon, Kate et al. · 2011 · Journal of Visualized Experiments · DOI

Full title: Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

Authors: Dickerson, Matthew T.; Leong, Kristen; Sheldon, Kate; Madison, Lara

Year: 2011

Venue: Journal of Visualized Experiments

DOI: https://doi.org/10.3791/2493

Paper Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts.

Buyel, Johannes F; Hubbuch, Jürgen; Fischer, Rainer · 2016 · Journal of visualized experiments : JoVE · DOI

Full title: Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts.

Authors: Buyel, Johannes F; Hubbuch, Jürgen; Fischer, Rainer

Year: 2016

Venue: Journal of visualized experiments : JoVE

DOI: https://doi.org/10.3791/54343

Paper Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

Dickerson, Matthew T.; Leong, Kristen; Sheldon, Kate et al. · 2011 · Journal of Visualized Experiments · DOI

Full title: Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

Authors: Dickerson, Matthew T.; Leong, Kristen; Sheldon, Kate; Madison, Lara

Year: 2011

Venue: Journal of Visualized Experiments

DOI: https://doi.org/10.3791/2493

Paper An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry.

Doneanu, Catalin; Fang, Jing; Alelyunas, Yun et al. · 2018 · Journal of visualized experiments : JoVE · DOI

Full title: An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry.

Authors: Doneanu, Catalin; Fang, Jing; Alelyunas, Yun; Yu, Ying Qing; Wrona, Mark; Chen, Weibin

Year: 2018

Venue: Journal of visualized experiments : JoVE

DOI: https://doi.org/10.3791/55325

Paper Host Cell Protein Analysis using Enrichment Beads Coupled with Limited Digestion.

Zhang, Sisi; Xiao, Hui; Li, Ning · 2024 · Journal of visualized experiments : JoVE · DOI

Full title: Host Cell Protein Analysis using Enrichment Beads Coupled with Limited Digestion.

Authors: Zhang, Sisi; Xiao, Hui; Li, Ning

Year: 2024

Venue: Journal of visualized experiments : JoVE

DOI: https://doi.org/10.3791/65544

Paper Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts.

Buyel, Johannes F; Hubbuch, Jürgen; Fischer, Rainer · 2016 · Journal of visualized experiments : JoVE · DOI

Full title: Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts.

Authors: Buyel, Johannes F; Hubbuch, Jürgen; Fischer, Rainer

Year: 2016

Venue: Journal of visualized experiments : JoVE

DOI: https://doi.org/10.3791/54343

Paper Establishment and initial validation of an ELISA method for determination of host cell protein in PHKC vaccines

Zhao, Yuxiu; Zhang, Yuelan; Wang, Hui et al. · 2010 · Int J Biologicals · DOI

Full title: Establishment and initial validation of an ELISA method for determination of host cell protein in PHKC vaccines

Authors: Zhao, Yuxiu; Zhang, Yuelan; Wang, Hui; Han, Xia; Ma, Ke; Lou, Shu-rong; Bai, Jiang; Zhao, Lanying

Year: 2010

Venue: Int J Biologicals

DOI: https://doi.org/10.3760/cma.j.issn.1673-4211.2010.01.003

Paper A microfluidic approach to high throughput quantification of host cell protein impurities for bioprocess development

Heo, Jun Hyuk; Mou, Xiaodun; Wang, Fengqiang et al. · 2014 · Pharmaceutical Bioprocessing · DOI

Full title: A microfluidic approach to high throughput quantification of host cell protein impurities for bioprocess development

Authors: Heo, Jun Hyuk; Mou, Xiaodun; Wang, Fengqiang; Troisi, John; Sandifer, Christopher W; Kirby, Suzanne L.; Driscoll, D. A.; Mercorelli, Suzanne; Pollard, David

Year: 2014

Venue: Pharmaceutical Bioprocessing

DOI: https://doi.org/10.4155/pbp.14.12

Paper Host cell protein dynamics in recombinant CHO cells: impacts from harvest to purification and beyond.

Hogwood, Catherine Em; Bracewell, Daniel G; Smales, C Mark · 2013 · Bioengineered · DOI

Full title: Host cell protein dynamics in recombinant CHO cells: impacts from harvest to purification and beyond.

Authors: Hogwood, Catherine Em; Bracewell, Daniel G; Smales, C Mark

Year: 2013

Venue: Bioengineered

DOI: https://doi.org/10.4161/bioe.23382

Paper The Comparison of Chemiluminescent- and Colorimetric-detection Based ELISA for Chinese Hamster Ovary Host Cell Proteins Quantification in Biotherapeutics

Driscoll, Fengqiang Wang Dennis · 2013 · Journal of Bioprocessing & Biotechniques · DOI

Full title: The Comparison of Chemiluminescent- and Colorimetric-detection Based ELISA for Chinese Hamster Ovary Host Cell Proteins Quantification in Biotherapeutics

Authors: Driscoll, Fengqiang Wang Dennis

Year: 2013

Venue: Journal of Bioprocessing & Biotechniques

DOI: https://doi.org/10.4172/2155-9821.1000136

Paper Getting CHO host cell protein analysis up to speed

Leiß, Michael; Meier, Monika; Pester, Oxana et al. · 2015 · Pharmaceutical Bioprocessing · DOI

Full title: Getting CHO host cell protein analysis up to speed

Authors: Leiß, Michael; Meier, Monika; Pester, Oxana; Aschner, Marion; Wedekind, Frank; Wiedmann, Michael; Wegele, Harald

Year: 2015

Venue: Pharmaceutical Bioprocessing

DOI: https://doi.org/10.4155/pbp.14.55

Paper Comprehensive tracking of host cell proteins during monoclonal antibody purifications using mass spectrometry.

Zhang, Qingchun; Goetze, Andrew M; Cui, Huanchun et al. · 2014 · mAbs · DOI

Full title: Comprehensive tracking of host cell proteins during monoclonal antibody purifications using mass spectrometry.

Authors: Zhang, Qingchun; Goetze, Andrew M; Cui, Huanchun; Wylie, Jenna; Trimble, Steve; Hewig, Art; Flynn, Gregory C

Year: 2014

Venue: mAbs

DOI: https://doi.org/10.4161/mabs.28120

Paper Process- and product-related impurities in the ChAdOx1 nCov-19 vaccine.

Krutzke, Lea; Rösler, Reinhild; Allmendinger, Ellen et al. · 2022 · eLife · DOI

Full title: Process- and product-related impurities in the ChAdOx1 nCov-19 vaccine.

Authors: Krutzke, Lea; Rösler, Reinhild; Allmendinger, Ellen; Engler, Tatjana; Wiese, Sebastian; Kochanek, Stefan

Year: 2022

Venue: eLife

DOI: https://doi.org/10.7554/eLife.78513

Paper Experimental characterization and prediction of Escherichia coli host cell proteome retention during preparative chromatography.

Disela, Roxana; Neijenhuis, Tim; Le Bussy, Olivier et al. · 2024 · Biotechnology and bioengineering · DOI

Full title: Experimental characterization and prediction of Escherichia coli host cell proteome retention during preparative chromatography.

Authors: Disela, Roxana; Neijenhuis, Tim; Le Bussy, Olivier; Geldhof, Geoffroy; Klijn, Marieke; Pabst, Martin; Ottens, Marcel

Year: 2024

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.28840

Paper A novel approach to evaluate ELISA antibody coverage of host cell proteins-combining ELISA-based immunocapture and mass spectrometry.

Pilely, Katrine; Nielsen, Solveig B; Draborg, Anette et al. · 2020 · Biotechnology progress · DOI

Full title: A novel approach to evaluate ELISA antibody coverage of host cell proteins-combining ELISA-based immunocapture and mass spectrometry.

Authors: Pilely, Katrine; Nielsen, Solveig B; Draborg, Anette; Henriksen, Maiken L; Hansen, Søren W K; Skriver, Lars; Mørtz, Ejvind; Lund, Rikke R

Year: 2020

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.2983

Paper Identification and classification of host cell proteins during biopharmaceutical process development.

Wilson, Louisa J; Lewis, Will; Kucia-Tran, Richard et al. · 2022 · Biotechnology progress · DOI

Full title: Identification and classification of host cell proteins during biopharmaceutical process development.

Authors: Wilson, Louisa J; Lewis, Will; Kucia-Tran, Richard; Bracewell, Daniel G

Year: 2022

Venue: Biotechnology progress

DOI: https://doi.org/10.1002/btpr.3224

Paper Identification and quantification of host cell protein impurities in biotherapeutics using mass spectrometry.

Schenauer, Matthew R; Flynn, Gregory C; Goetze, Andrew M · 2012 · Analytical biochemistry · DOI

Full title: Identification and quantification of host cell protein impurities in biotherapeutics using mass spectrometry.

Authors: Schenauer, Matthew R; Flynn, Gregory C; Goetze, Andrew M

Year: 2012

Venue: Analytical biochemistry

DOI: https://doi.org/10.1016/j.ab.2012.05.018

Paper Development of continuous processing platform utilizing aqueous two-phase extraction for purification of monoclonal antibodies.

Anupa, Anupa; Metya, Subhankar; Mihooliya, Kanti N et al. · 2024 · Journal of chromatography. A · DOI

Full title: Development of continuous processing platform utilizing aqueous two-phase extraction for purification of monoclonal antibodies.

Authors: Anupa, Anupa; Metya, Subhankar; Mihooliya, Kanti N; Rathore, Anurag S

Year: 2024

Venue: Journal of chromatography. A

DOI: https://doi.org/10.1016/j.chroma.2023.464605

Paper Efficient host cell protein clearance: A study of membrane adsorbers and resins in biopharmaceutical processes.

Šprager, Ernest; Reisinger, Veronika; Sommer, Jonas et al. · 2025 · Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · DOI

Full title: Efficient host cell protein clearance: A study of membrane adsorbers and resins in biopharmaceutical processes.

Authors: Šprager, Ernest; Reisinger, Veronika; Sommer, Jonas; Lekić, Tinkara; Pucelj, Nina; Kljun, Tina; Lunder, Mojca; Vašl, Jožica; Bratkovič, Tomaž

Year: 2025

Venue: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

DOI: https://doi.org/10.1016/j.jchromb.2025.124796

Paper A new deep field scan liquid chromatography-mass spectrometry method to identify host cell proteins in therapeutic proteins.

Wang, Leo; Barton, Chris; Wang, Yan et al. · 2026 · Journal of pharmaceutical and biomedical analysis · DOI

Full title: A new deep field scan liquid chromatography-mass spectrometry method to identify host cell proteins in therapeutic proteins.

Authors: Wang, Leo; Barton, Chris; Wang, Yan; Sessler, Nicole; Wang, Larry

Year: 2026

Venue: Journal of pharmaceutical and biomedical analysis

DOI: https://doi.org/10.1016/j.jpba.2025.117164

Paper Analysis for residual host cell proteins and DNA in process streams of a recombinant protein product expressed in Escherichia coli cells.

Rathore, Anurag Singh; Sobacke, S E; Kocot, T J et al. · 2003 · Journal of pharmaceutical and biomedical analysis · DOI

Full title: Analysis for residual host cell proteins and DNA in process streams of a recombinant protein product expressed in Escherichia coli cells.

Authors: Rathore, Anurag Singh; Sobacke, S E; Kocot, T J; Morgan, D R; Dufield, R L; Mozier, N M

Year: 2003

Venue: Journal of pharmaceutical and biomedical analysis

DOI: https://doi.org/10.1016/s0731-7085(03)00157-2

Paper Improved Host Cell Protein Analysis in Monoclonal Antibody Products through Molecular Weight Cutoff Enrichment.

Chen, I-Hsuan; Xiao, Hui; Daly, Thomas et al. · 2020 · Analytical chemistry · DOI

Full title: Improved Host Cell Protein Analysis in Monoclonal Antibody Products through Molecular Weight Cutoff Enrichment.

Authors: Chen, I-Hsuan; Xiao, Hui; Daly, Thomas; Li, Ning

Year: 2020

Venue: Analytical chemistry

DOI: https://doi.org/10.1021/acs.analchem.9b05081

Paper Toward the complete characterization of host cell proteins in biotherapeutics via affinity depletions, LC-MS/MS, and multivariate analysis.

Madsen, James A; Farutin, Victor; Carbeau, Theresa et al. · 2015 · mAbs · DOI

Full title: Toward the complete characterization of host cell proteins in biotherapeutics via affinity depletions, LC-MS/MS, and multivariate analysis.

Authors: Madsen, James A; Farutin, Victor; Carbeau, Theresa; Wudyka, Steve; Yin, Yan; Smith, Stephen; Anderson, James; Capila, Ishan

Year: 2015

Venue: mAbs

DOI: https://doi.org/10.1080/19420862.2015.1082017

Paper Advanced assessment of the physicochemical characteristics of Remicade® and Inflectra® by sensitive LC/MS techniques.

Fang, Jing; Doneanu, Catalin; Alley, William R et al. · 2016 · mAbs · DOI

Full title: Advanced assessment of the physicochemical characteristics of Remicade® and Inflectra® by sensitive LC/MS techniques.

Authors: Fang, Jing; Doneanu, Catalin; Alley, William R; Yu, Ying Qing; Beck, Alain; Chen, Weibin

Year: 2016

Venue: mAbs

DOI: https://doi.org/10.1080/19420862.2016.1193661

Paper An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry.

Doneanu, Catalin; Fang, Jing; Alelyunas, Yun et al. · 2018 · Journal of visualized experiments : JoVE · DOI

Full title: An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry.

Authors: Doneanu, Catalin; Fang, Jing; Alelyunas, Yun; Yu, Ying Qing; Wrona, Mark; Chen, Weibin

Year: 2018

Venue: Journal of visualized experiments : JoVE

DOI: https://doi.org/10.3791/55325

Paper Analysis of host-cell proteins in biotherapeutic proteins by comprehensive online two-dimensional liquid chromatography/mass spectrometry.

Doneanu, Catalin E; Xenopoulos, Alex; Fadgen, Keith et al. · 2012 · mAbs · DOI

Full title: Analysis of host-cell proteins in biotherapeutic proteins by comprehensive online two-dimensional liquid chromatography/mass spectrometry.

Authors: Doneanu, Catalin E; Xenopoulos, Alex; Fadgen, Keith; Murphy, Jim; Skilton, St John; Prentice, Holly; Stapels, Martha; Chen, Weibin

Year: 2012

Venue: mAbs

DOI: https://doi.org/10.4161/mabs.4.1.18748

Thesis Characterization and optimization of a CHO antibody manufacturing process

Reger, Lucas Nik · 2025 · Wageningen University & Research · DOI

Full title: Characterization and optimization of a CHO antibody manufacturing process

Authors: Reger, Lucas Nik

Year: 2025

Venue: Wageningen University & Research

DOI: https://research.wur.nl/en/publications/characterization-and-optimization-of-a-cho-antibody-manufacturing

Thesis CUSTOMIZED QUANTIFICATION OF HOST CELL PROTEIN WITH BIO-LAYER INTERFEROMETRY

Sjöblom, Eva · 2020 · Lund University Student Papers · DOI

Full title: CUSTOMIZED QUANTIFICATION OF HOST CELL PROTEIN WITH BIO-LAYER INTERFEROMETRY

Authors: Sjöblom, Eva

Year: 2020

Venue: Lund University Student Papers

DOI: http://lup.lub.lu.se/student-papers/record/9032572

Thesis Detection of Hepatitis B Core Antigen by Phage Display Mediated Immuno-Taqman Real-Time Polymerase Chain Reaction

Monjezi, Razieh · 2011 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Detection of Hepatitis B Core Antigen by Phage Display Mediated Immuno-Taqman Real-Time Polymerase Chain Reaction

Authors: Monjezi, Razieh

Year: 2011

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: oai:psasir.upm.edu.my:19454

Thesis Impact of Nanoparticle Aggregation on Protein Recovery through a Pentadentate Chelate Ligand on Magnetic Carriers

Fraga García, P.; Freiherr von Roman, M.; Reinlein, S. et al. · 2014 · Technical University of Munich (TUM) · DOI

Full title: Impact of Nanoparticle Aggregation on Protein Recovery through a Pentadentate Chelate Ligand on Magnetic Carriers

Authors: Fraga García, P.; Freiherr von Roman, M.; Reinlein, S.; Wolf, M.; Berensmeier, S.

Year: 2014

Venue: Technical University of Munich (TUM)

DOI: https://mediatum.ub.tum.de/1230644

Thesis Magnetic one-step purification of his-tagged protein by bare iron oxide nanoparticles

Schwaminger SP, Fraga-García P, Blank-Shim SA, Straub T, Haslbeck M, Muraca F, Dawson KA, Berensmeier S: · 2019 · Technical University of Munich (TUM) · DOI

Full title: Magnetic one-step purification of his-tagged protein by bare iron oxide nanoparticles

Authors: Schwaminger SP, Fraga-García P, Blank-Shim SA, Straub T, Haslbeck M, Muraca F, Dawson KA, Berensmeier S:

Year: 2019

Venue: Technical University of Munich (TUM)

DOI: https://mediatum.ub.tum.de/1507194

Thesis Impact of the substrate viscosity, potentially interfering proteins and further sample characteristics on the ion exchange efficiency of tangential flow membrane adsorbers

Brand, J.; Kulozik, U.; Brand, Janina et al. · 2017 · Technical University of Munich (TUM) · DOI

Full title: Impact of the substrate viscosity, potentially interfering proteins and further sample characteristics on the ion exchange efficiency of tangential flow membrane adsorbers

Authors: Brand, J.;Kulozik, U.;Brand, Janina;Kulozik, Ulrich

Year: 2017

Venue: Technical University of Munich (TUM)

DOI: https://mediatum.ub.tum.de/1467575

Thesis Selective release of overexpressed recombinant proteins from E. coli cells facilitates one-step chromatographic purification of peptide-tagged green fluorescent protein variants

Kaveh-Baghbaderani Y, Blank-Shim SA, Koch T, Berensmeier S: · 2018 · Technical University of Munich (TUM) · DOI

Full title: Selective release of overexpressed recombinant proteins from E. coli cells facilitates one-step chromatographic purification of peptide-tagged green fluorescent protein variants

Authors: Kaveh-Baghbaderani Y, Blank-Shim SA, Koch T, Berensmeier S:

Year: 2018

Venue: Technical University of Munich (TUM)

DOI: https://mediatum.ub.tum.de/1468923

Thesis Purification of a peptide tagged protein via an affinity chromatographic process with underivatized silica

Rauwolf S, Steegmüller T, Schwaminger SP, Berensmeier S: · 2021 · Technical University of Munich (TUM) · DOI

Full title: Purification of a peptide tagged protein via an affinity chromatographic process with underivatized silica

Authors: Rauwolf S, Steegmüller T, Schwaminger SP, Berensmeier S:

Year: 2021

Venue: Technical University of Munich (TUM)

DOI: https://mediatum.ub.tum.de/1615008

Thesis Pseudo-affinity capture of K. phaffii host cell proteins in flow-through mode: purification of protein therapeutics and proteomic study

Sripada, Sobhana A.; Elhanafi, Driss; Collins, Leonard B. et al. · 2023 · Technical University of Denmark (DTU) · DOI

Full title: Pseudo-affinity capture of K. phaffii host cell proteins in flow-through mode: purification of protein therapeutics and proteomic study

Authors: Sripada, Sobhana A.; Elhanafi, Driss; Collins, Leonard B.; Williams, Taufika I.; Linova, Marina Y.; id_orcid 0000-0001-7716-1257; Woodley, John M.; id_orcid 0000-0002-7976-2483; Boi, Cristiana; Menegatti, Stefano

Year: 2023

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/7581505e-4aa5-44f4-8a87-2f0ddf89bb09

Thesis Novel multimodal cation-exchange membrane for the purification of a single-chain variable fragment from Pichia pastoris supernatant

Pham, Dan N.; id_orcid 0000-0002-7804-3882; Linova, Marina Y. et al. · 2024 · Technical University of Denmark (DTU) · DOI

Full title: Novel multimodal cation-exchange membrane for the purification of a single-chain variable fragment from Pichia pastoris supernatant

Authors: Pham, Dan N.; id_orcid 0000-0002-7804-3882; Linova, Marina Y.; id_orcid 0000-0001-7716-1257; Smith, William K.; Brown, Hunter; Elhanafi, Driss; Fan, Jinxin; Lavoie, Joseph; Woodley, John M.; id_orcid 0000-0002-7976-2483; Carbonell, Ruben G.

Year: 2024

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/d389f2ef-aa9b-44aa-ab2a-6cc339b6945f

Thesis Purification of a monoclonal antibody using a novel high-capacity multimodal cation exchange nonwoven membrane

Fan, Jinxin; Sripada, Sobhana A.; Pham, Dan N. et al. · 2023 · Technical University of Denmark (DTU) · DOI

Full title: Purification of a monoclonal antibody using a novel high-capacity multimodal cation exchange nonwoven membrane

Authors: Fan, Jinxin; Sripada, Sobhana A.; Pham, Dan N.; id_orcid 0000-0002-7804-3882; Linova, Marina Y.; id_orcid 0000-0001-7716-1257; Woodley, John M.; id_orcid 0000-0002-7976-2483; Menegatti, Stefano; Boi, Cristiana; Carbonell, Ruben G.

Year: 2023

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/1a0d8bf1-9610-4ba3-9bbf-5ca351b1630c

Thesis Peptide ligands for the universal purification of exosomes by affinity chromatography

Kilgore, Ryan E.; Moore, Brandyn D.; Sripada, Sobhana A. et al. · 2024 · Technical University of Denmark (DTU) · DOI

Full title: Peptide ligands for the universal purification of exosomes by affinity chromatography

Authors: Kilgore, Ryan E.; Moore, Brandyn D.; Sripada, Sobhana A.; Chu, Wenning; Shastry, Shriarjun; Barbieri, Eduardo; Hu, Shiqi; Tian, Weihua; id_orcid 0000-0003-2357-3947; Petersen, Heidi; id_orcid 0009-0008-0527-4728; Mohammadifar, Mohammad; id_orcid 0000-0001-8804-4873; Simpson, Aryssa; Brown, Ashley; Lavoie, Joseph; Elhanafi, Driss; Goletz, Steffen; id_orcid 0000-0003-1463-5448; Cheng, Ke; Daniele, Michael A.; Menegatti, Stefano

Year: 2024

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/3f1f895f-882d-4af0-9a23-9c6dbfc64c59

Thesis Multiplex secretome engineering enhances recombinant protein production and purity

Kol, Stefan; Ley, Daniel; Wulff, Tune et al. · 2020 · Technical University of Denmark (DTU) · DOI

Full title: Multiplex secretome engineering enhances recombinant protein production and purity

Authors: Kol, Stefan; Ley, Daniel; Wulff, Tune; Decker, Marianne; Arnsdorf, Johnny; id_orcid 0000-0002-2738-0811; Schoffelen, Sanne; id_orcid 0000-0003-2664-8561; Hansen, Anders Holmgaard; id_orcid 0000-0002-6007-4787; Jensen, Tanja Lyholm; Gutierrez, Jahir M.; Chiang, Austin W. T.; Masson, Helen O.; Palsson, Bernhard O.; id_orcid 0000-0003-2357-6785; Voldborg, Bjørn Gunnar; id_orcid 0000-0002-7005-1642; Pedersen, Lasse Ebdrup; id_orcid 0000-0002-6064-919X; Kildegaard, Helene Faustrup; Min Lee, Gyun; Lewis, Nathan E.

Year: 2020

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/c440cd45-e242-4b63-9b03-861ba7d54bf2

Thesis Peptide ligands for the universal purification of exosomes by affinity chromatography

Kilgore, Ryan E.; Moore, Brandyn D.; Sripada, Sobhana A. et al. · 2024 · Technical University of Denmark (DTU) · DOI

Full title: Peptide ligands for the universal purification of exosomes by affinity chromatography

Year: 2024

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/3f1f895f-882d-4af0-9a23-9c6dbfc64c59

Thesis Purification of a monoclonal antibody using a novel high-capacity multimodal cation exchange nonwoven membrane

Fan, Jinxin; Sripada, Sobhana A.; Pham, Dan N. et al. · 2023 · Technical University of Denmark (DTU) · DOI

Full title: Purification of a monoclonal antibody using a novel high-capacity multimodal cation exchange nonwoven membrane

Year: 2023

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/1a0d8bf1-9610-4ba3-9bbf-5ca351b1630c

Thesis A Systematic Framework to Optimize and Control Monoclonal Antibody Manufacturing Process

Li, Ying Fei · 2018 · Columbia University · DOI

Full title: A Systematic Framework to Optimize and Control Monoclonal Antibody Manufacturing Process

Authors: Li, Ying Fei

Year: 2018

Venue: Columbia University

DOI: https://doi.org/10.7916/D8GX5TJP

Thesis 3D printed platform stationary phases for downstream processing: development of porous platform inks, characterization of 3D printed chromatography columns and testing for industrial bioprocessing

Conti, Mariachiara · 2024 · The University of Edinburgh · DOI

Full title: 3D printed platform stationary phases for downstream processing: development of porous platform inks, characterization of 3D printed chromatography columns and testing for industrial bioprocessing

Authors: Conti, Mariachiara

Year: 2024

Venue: The University of Edinburgh

DOI: https://hdl.handle.net/1842/42110

Thesis The Regulation of Peptide Mimotope/Epitope Recognition by Monoclonal Antibodies

Smith, Richard Gary · 2002 · University of Nottingham · DOI

Full title: The Regulation of Peptide Mimotope/Epitope Recognition by Monoclonal Antibodies

Authors: Smith, Richard Gary

Year: 2002

Venue: University of Nottingham

DOI: https://eprints.nottingham.ac.uk/10053/1/RGSmithThesis2002.pdf

Thesis Analysis of extracellular vesicle and particulate production by CHO cells in culture: evaluating the risk to the efficiency of downstream purification operations

Tsimperdonis, Alexandros · 2022 · White Rose eTheses (Leeds, Sheffield, York) · DOI

Full title: Analysis of extracellular vesicle and particulate production by CHO cells in culture: evaluating the risk to the efficiency of downstream purification operations

Authors: Tsimperdonis, Alexandros

Year: 2022

Venue: White Rose eTheses (Leeds, Sheffield, York)

DOI: oai:etheses.whiterose.ac.uk:37284

Thesis Proof-of-concept analytical instrument for label-free optical deconvolution of protein species in a mixture

Hales, John E.; Aoudjane, Samir; Aeppli, Gabriel et al. · 2021 · École polytechnique fédérale de Lausanne (EPFL) · DOI

Full title: Proof-of-concept analytical instrument for label-free optical deconvolution of protein species in a mixture

Authors: Hales, John E.; Aoudjane, Samir; Aeppli, Gabriel; Dalby, Paul A.

Year: 2021

Venue: École polytechnique fédérale de Lausanne (EPFL)

DOI: https://infoscience.epfl.ch/handle/20.500.14299/177182

Thesis Cascading effect in bioprocessing – the impact of mild hypothermia on CHO cell behavior and host cell protein composition

Goey, CH; Tsang, J; Bell, D et al. · 2017 · Imperial College London · DOI

Full title: Cascading effect in bioprocessing – the impact of mild hypothermia on CHO cell behavior and host cell protein composition

Authors: Goey, CH; Tsang, J; Bell, D; Kontoravdi, C

Year: 2017

Venue: Imperial College London

DOI: https://onlinelibrary.wiley.com/doi/epdf/10.1002/bit.26437

Thesis In-column ATR-FTIR spectroscopy to monitor affinity chromatography purification of monoclonal antibodies

Boulet-Audet, M; Kazarian, SG; Byrne, B · 2016 · Imperial College London · DOI

Full title: In-column ATR-FTIR spectroscopy to monitor affinity chromatography purification of monoclonal antibodies

Authors: Boulet-Audet, M; Kazarian, SG; Byrne, B

Year: 2016

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/34378

Thesis CHO cell cultures in shake flasks and bioreactors present different host cell protein profiles in the supernatant

Goey, CH; Bell, D; Kontoravdi, C · 2019 · Imperial College London · DOI

Full title: CHO cell cultures in shake flasks and bioreactors present different host cell protein profiles in the supernatant

Authors: Goey, CH; Bell, D; Kontoravdi, C

Year: 2019

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/67420

Thesis Cascading effects in bioprocessing: the impact of cell culture environment on CHO cell behaviour and host cell protein species

Goey, Cher Hui · 2017 · Imperial College London · DOI

Full title: Cascading effects in bioprocessing: the impact of cell culture environment on CHO cell behaviour and host cell protein species

Authors: Goey, Cher Hui

Year: 2017

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/52703

Thesis Mild hypothermic culture conditions impact residual host cell protein composition post-protein a chromatography

Goey, CH; Bell, David; Kontoravdi, K · 2018 · Imperial College London · DOI

Full title: Mild hypothermic culture conditions impact residual host cell protein composition post-protein a chromatography

Authors: Goey, CH; Bell, David; Kontoravdi, K

Year: 2018

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/63191

Thesis Population balance modelling captures host cell protein dynamics in CHO cell cultures

Alhuthali, Sakhr; Kontoravdi, Cleo · 2022 · Imperial College London · DOI

Full title: Population balance modelling captures host cell protein dynamics in CHO cell cultures

Authors: Alhuthali, Sakhr; Kontoravdi, Cleo

Year: 2022

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/96277

Thesis P. pastoris (K. phaffi) perfusion processes: evaluation of cell retention techniques and integrated purification strategies

Linova, M.; id_orcid 0000-0001-7716-1257; Kodiripaka, S. et al. · 2025 · Technical University of Denmark (DTU) · DOI

Full title: P. pastoris (K. phaffi) perfusion processes: evaluation of cell retention techniques and integrated purification strategies

Authors: Linova, M.; id_orcid 0000-0001-7716-1257; Kodiripaka, S.; Martins, E.; Pham, D.; id_orcid 0000-0002-7804-3882; Sripada, S.; Menegatti, S.; Carbonell, R.; Woodley, J.; id_orcid 0000-0002-7976-2483

Year: 2025

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/2d3b56e0-24f0-449e-bda9-fd8ea452eaaf

Thesis Pseudo-affinity capture of K. phaffii host cell proteins in flow-through mode: purification of protein therapeutics and proteomic study

Sripada, Sobhana A.; Elhanafi, Driss; Collins, Leonard B. et al. · 2023 · Technical University of Denmark (DTU) · DOI

Full title: Pseudo-affinity capture of K. phaffii host cell proteins in flow-through mode: purification of protein therapeutics and proteomic study

Year: 2023

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/7581505e-4aa5-44f4-8a87-2f0ddf89bb09

Thesis Elucidation of the CHO Super-Ome (CHO-SO) by Proteoinformatics

Kumar, Amit; Baycin-Hizal, Deniz; Wolozny, Daniel et al. · 2015 · Technical University of Denmark (DTU) · DOI

Full title: Elucidation of the CHO Super-Ome (CHO-SO) by Proteoinformatics

Authors: Kumar, Amit; Baycin-Hizal, Deniz; Wolozny, Daniel; Pedersen, Lasse Ebdrup; id_orcid 0000-0002-6064-919X; Lewis, Nathan; Heffner, Kelley; Chaerkady, Raghothama; Cole, Robert N.; Shiloach, Joseph; Zhang, Hui; Bowen, Michael A.; Betenbaugh, Michael J.

Year: 2015

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/9d841c9b-1302-4b62-b81d-5087d0e2af07

Thesis Use of Continuous Magnetic Extraction for removal of feedstock contaminants in flow-through mode

Paulus, Anja; Fischer, Ingo; Hobley, Timothy John et al. · 2014 · Technical University of Denmark (DTU) · DOI

Full title: Use of Continuous Magnetic Extraction for removal of feedstock contaminants in flow-through mode

Authors: Paulus, Anja; Fischer, Ingo; Hobley, Timothy John; id_orcid 0000-0002-1355-6214; Franzreb, Matthias

Year: 2014

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/7c2b7274-40ad-40a0-9bb0-61dea52269eb

Thesis Multiplex secretome engineering enhances recombinant protein production and purity

Kol, Stefan; Ley, Daniel; Wulff, Tune et al. · 2020 · Technical University of Denmark (DTU) · DOI

Full title: Multiplex secretome engineering enhances recombinant protein production and purity

Year: 2020

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/c440cd45-e242-4b63-9b03-861ba7d54bf2

Thesis Purification of green fluorescent protein using fast centrifugal partition chromatography

Soares, Bruna P.; Santos, João H.P.M.; Martins, Margarida et al. · 2021 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Purification of green fluorescent protein using fast centrifugal partition chromatography

Authors: Soares, Bruna P.; Santos, João H.P.M.; Martins, Margarida; Almeida, Mafalda R.; Santos, Nathalia V. [UNESP]; Freire, Mara G.; Santos-Ebinuma, Valéria C. [UNESP]; Coutinho, João A.P.; Pereira, Jorge F.B. [UNESP]; Ventura, Sónia P.M.

Year: 2021

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://dx.doi.org/10.1016/j.seppur.2020.117648

Thesis Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 2. Nonionic micellar systems

Teixeira-Pinto, Renata Garcia Rodrigues; Molino, João Vitor Dutra; Santos-Ebinuma, Valéria Carvalho [UNESP] et al. · 2019 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Effect of electrolytes as adjuvants in GFP and LPS partitioning on aqueous two-phase systems: 2. Nonionic micellar systems

Authors: Teixeira-Pinto, Renata Garcia Rodrigues; Molino, João Vitor Dutra; Santos-Ebinuma, Valéria Carvalho [UNESP]; Pessoa, Adalberto; Valentini, Sandro Roberto; Pereira, Jorge Fernando Brandão [UNESP]; Lopes, André Moreni [UNESP]

Year: 2019

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://dx.doi.org/10.1016/j.seppur.2018.07.078

Thesis Aqueous Biphasic Systems Composed of Cholinium Chloride and Polymers as Effective Platforms for the Purification of Recombinant Green Fluorescent Protein

Dos Santos, Nathalia V. [UNESP]; Martins, Margarida; Santos-Ebinuma, Valéria C. [UNESP] et al. · 2018 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Aqueous Biphasic Systems Composed of Cholinium Chloride and Polymers as Effective Platforms for the Purification of Recombinant Green Fluorescent Protein

Authors: Dos Santos, Nathalia V. [UNESP]; Martins, Margarida; Santos-Ebinuma, Valéria C. [UNESP]; Ventura, Sónia P. M.; Coutinho, Joaìo A. P.; Valentini, Sandro R. [UNESP]; Pereira, Jorge F. B. [UNESP]

Year: 2018

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://dx.doi.org/10.1021/acssuschemeng.8b01730

Thesis Development of functional living materials for monoclonal antibody purification

Lee, Juwon · 2024 · Hong Kong University of Science and Technology (HKUST) · DOI

Full title: Development of functional living materials for monoclonal antibody purification

Authors: Lee, Juwon

Year: 2024

Venue: Hong Kong University of Science and Technology (HKUST)

DOI: http://repository.hkust.edu.hk/ir/Record/1783.1-144133

Thesis A Precipitation-Based Process Principle for Purification of an Industrially Relevant Therapeutic Monoclonal Antibody and Fc-Fusion Protein

Overgaard, Rasmus · 2025 · Hong Kong University of Science and Technology (HKUST) · DOI

Full title: A Precipitation-Based Process Principle for Purification of an Industrially Relevant Therapeutic Monoclonal Antibody and Fc-Fusion Protein

Authors: Overgaard, Rasmus

Year: 2025

Venue: Hong Kong University of Science and Technology (HKUST)

DOI: http://repository.hkust.edu.hk/ir/Record/1783.1-152479

Thesis Fc-binding Hexamer Peptide Ligands for Immunoglobulin Purification

Yang, Haiou · 2010 · North Carolina State University · DOI

Full title: Fc-binding Hexamer Peptide Ligands for Immunoglobulin Purification

Authors: Yang, Haiou

Year: 2010

Venue: North Carolina State University

DOI: http://www.lib.ncsu.edu/resolver/1840.16/3655

Thesis Causes of industrial protein a column degradation, explored using Raman spectroscopy.

Beattie, James W; Istrate, Alena; Lu, Annabelle et al. · 2022 · Imperial College London · DOI

Full title: Causes of industrial protein a column degradation, explored using Raman spectroscopy.

Authors: Beattie, James W; Istrate, Alena; Lu, Annabelle; Marshall, Cameron; Rowland-Jones, Ruth C; Farys, Monika; Kazarian, Sergei G; Byrne, Bernadette

Year: 2022

Venue: Imperial College London

DOI: https://www.ncbi.nlm.nih.gov/pubmed/36318727

Thesis A model-based approach towards accelerated process development: a case study on chromatography

Sachio, Steven; Kontoravdi, Cleo; Papathanasiou, Maria M · 2024 · Imperial College London · DOI

Full title: A model-based approach towards accelerated process development: a case study on chromatography

Authors: Sachio, Steven; Kontoravdi, Cleo; Papathanasiou, Maria M

Year: 2024

Venue: Imperial College London

DOI: https://www.sciencedirect.com/science/article/pii/S0263876223005166

Thesis Monitoring of biotherapeutic purification by vibrational spectroscopy

Beattie, James · 2025 · Imperial College London · DOI

Full title: Monitoring of biotherapeutic purification by vibrational spectroscopy

Authors: Beattie, James

Year: 2025

Venue: Imperial College London

DOI: https://hdl.handle.net/10044/1/123044

Thesis In vivo and in vitro cleavages of inteins through modifications of terminal regions and revisions of environmental factors

Wang, Hao · 2019 · Hong Kong University of Science and Technology (HKUST) · DOI

Full title: In vivo and in vitro cleavages of inteins through modifications of terminal regions and revisions of environmental factors

Authors: Wang, Hao

Year: 2019

Venue: Hong Kong University of Science and Technology (HKUST)

DOI: http://repository.hkust.edu.hk/ir/Record/1783.1-100066

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2. Data Extraction

0/421 extracted

Paper

Polysorbates degrading enzymes in biotherapeutics - a current status and future perspectives.

Felix, Marius Nicolaus; Waerner, Thomas; Lakatos, Daniel et al. · 2024

Full title: Polysorbates degrading enzymes in biotherapeutics - a current status and future perspectives.

Authors: Felix, Marius Nicolaus; Waerner, Thomas; Lakatos, Daniel; Reisinger, Bernd; Fischer, Simon; Garidel, Patrick

DOI: https://doi.org/10.3389/fbioe.2024.1490276

Paper

Label-free quantification of host cell protein impurity in recombinant hemoglobin materials

Henrion, André; Arsene, Cristian; Liebl, Maik et al. · 2023

Full title: Label-free quantification of host cell protein impurity in recombinant hemoglobin materials

Authors: Henrion, André; Arsene, Cristian; Liebl, Maik; O’Connor, Gavin

DOI: https://doi.org/10.1007/s00216-023-05024-8

Paper

Exploring sample preparation and data evaluation strategies for enhanced identification of host cell proteins in drug products of therapeutic antibodies and Fc-fusion proteins.

Esser-Skala, Wolfgang; Segl, Marius; Wohlschlager, Therese et al. · 2020

Full title: Exploring sample preparation and data evaluation strategies for enhanced identification of host cell proteins in drug products of therapeutic antibodies and Fc-fusion proteins.

Authors: Esser-Skala, Wolfgang; Segl, Marius; Wohlschlager, Therese; Reisinger, Veronika; Holzmann, Johann; Huber, Christian G

DOI: https://doi.org/10.1007/s00216-020-02796-1

Paper

Monitoring process-related impurities in biologics-host cell protein analysis.

Pilely, Katrine; Johansen, Martin Rask; Lund, Rikke Raaen et al. · 2022

Full title: Monitoring process-related impurities in biologics-host cell protein analysis.

Authors: Pilely, Katrine; Johansen, Martin Rask; Lund, Rikke Raaen; Kofoed, Thomas; Jørgensen, Thomas Kjærsgaard; Skriver, Lars; Mørtz, Ejvind

DOI: https://doi.org/10.1007/s00216-021-03648-2

Paper

Multiplex secretome engineering enhances recombinant protein production and purity.

Kol, Stefan; Ley, Daniel; Wulff, Tune et al. · 2020

Full title: Multiplex secretome engineering enhances recombinant protein production and purity.

DOI: https://doi.org/10.1038/s41467-020-15866-w

Paper

Proteomic Analysis of Host Cell Protein Dynamics in the Culture Supernatants of Antibody-Producing CHO Cells.

Park, Jin Hyoung; Jin, Jong Hwa; Lim, Myung Sin et al. · 2017

Full title: Proteomic Analysis of Host Cell Protein Dynamics in the Culture Supernatants of Antibody-Producing CHO Cells.

Authors: Park, Jin Hyoung; Jin, Jong Hwa; Lim, Myung Sin; An, Hyun Joo; Kim, Jong Won; Lee, Gyun Min

DOI: https://doi.org/10.1038/srep44246

Paper

Identification and characterization of a residual host cell protein hexosaminidase B associated with N-glycan degradation during the stability study of a therapeutic recombinant monoclonal antibody product.

Li, Xuanwen; An, Yan; Liao, Jing et al. · 2021

DOI: https://doi.org/10.1002/btpr.3128

Paper

Definitive benchmarking of DDA and DIA for host cell protein analysis on the Orbitrap Astral in a regulatory-aligned framework

Khalil, Somar; Plisnier, Michel; Plisnier, Michel · 2025

Full title: Definitive benchmarking of DDA and DIA for host cell protein analysis on the Orbitrap Astral in a regulatory-aligned framework

Authors: Khalil, Somar; Plisnier, Michel; Plisnier, Michel

DOI: https://doi.org/10.1101/2025.07.31.667876

Paper

Mass spectrometry-based investigation of measles and mumps virus proteome.

Sviben, Dora; Forcic, Dubravko; Halassy, Beata et al. · 2018

Full title: Mass spectrometry-based investigation of measles and mumps virus proteome.

Authors: Sviben, Dora; Forcic, Dubravko; Halassy, Beata; Allmaier, Günter; Marchetti-Deschmann, Martina; Brgles, Marija

DOI: https://doi.org/10.1186/s12985-018-1073-9

Paper

Identification and Monitoring of Host Cell Proteins by Mass Spectrometry Combined with High Performance Immunochemistry Testing

Bomans, Katrin; Lang, Antje; Roedl, Veronika et al. · 2013

Full title: Identification and Monitoring of Host Cell Proteins by Mass Spectrometry Combined with High Performance Immunochemistry Testing

DOI: https://doi.org/10.1371/journal.pone.0081639

Paper

Effective flow-through polishing strategies for knob-into-hole bispecific antibodies.

Chen, Serene W; Hoi, Kong Meng; Mahfut, Farouq Bin et al. · 2022

Full title: Effective flow-through polishing strategies for knob-into-hole bispecific antibodies.

Authors: Chen, Serene W; Hoi, Kong Meng; Mahfut, Farouq Bin; Yang, Yuansheng; Zhang, Wei

DOI: https://doi.org/10.1186/s40643-022-00590-8

Paper

Immunoinformatic Risk Assessment of Host Cell Proteins During Process Development for Biologic Therapeutics.

Haltaufderhyde, Kirk; Roberts, Brian J; Khan, Sundos et al. · 2023

Full title: Immunoinformatic Risk Assessment of Host Cell Proteins During Process Development for Biologic Therapeutics.

Authors: Haltaufderhyde, Kirk; Roberts, Brian J; Khan, Sundos; Terry, Frances; Boyle, Christine M; McAllister, Mitchell; Martin, William; Rosenberg, Amy; De Groot, Anne S

DOI: https://doi.org/10.1208/s12248-023-00852-z

Paper

Population balance modelling captures host cell protein dynamics in CHO cell cultures.

Alhuthali, Sakhr; Kontoravdi, Cleo · 2022

Full title: Population balance modelling captures host cell protein dynamics in CHO cell cultures.

Authors: Alhuthali, Sakhr; Kontoravdi, Cleo

DOI: https://doi.org/10.1371/journal.pone.0265886

Paper

Assessment of the immunogenicity of residual host cell protein impurities of OsrHSA.

Abiri, Naghmeh; Pang, Jianlei; Ou, Jiquan et al. · 2018

Full title: Assessment of the immunogenicity of residual host cell protein impurities of OsrHSA.

Authors: Abiri, Naghmeh; Pang, Jianlei; Ou, Jiquan; Shi, Bo; Wang, Xianghong; Zhang, Sucai; Sun, Yunxia; Yang, Daichang

DOI: https://doi.org/10.1371/journal.pone.0193339

Paper

Leveraging an advanced simulated moving bed approach to achieve 3-component separation for enhanced impurity removal in a non-affinity cation exchange capture step.

Chen, Serene W; Zheng, Zi Ying; Mahfut, Farouq Bin et al. · 2023

Full title: Leveraging an advanced simulated moving bed approach to achieve 3-component separation for enhanced impurity removal in a non-affinity cation exchange capture step.

Authors: Chen, Serene W; Zheng, Zi Ying; Mahfut, Farouq Bin; Yang, Yuansheng; Ogino, Masahiro; Okada, Kazuo; Sato, Kohei; Zhang, Wei

DOI: https://doi.org/10.1371/journal.pone.0280760

Paper

Thermostable chaperone-based polypeptide biosynthesis: Enfuvirtide model product quality and protocol-related impurities.

Zenin, Vladimir; Tsedilin, Andrey; Yurkova, Maria et al. · 2023

Full title: Thermostable chaperone-based polypeptide biosynthesis: Enfuvirtide model product quality and protocol-related impurities.

Authors: Zenin, Vladimir; Tsedilin, Andrey; Yurkova, Maria; Siniavin, Andrey; Fedorov, Alexey

DOI: https://doi.org/10.1371/journal.pone.0286752

Paper

Towards a cleaner CHO chassis: systematic knockout of host cell proteins for efficient biopharmaceutical manufacturing.

Marzluf, Jannis Peter; Klein, Jennifer; Kirchmeier, Daniela et al. · 2026

Full title: Towards a cleaner CHO chassis: systematic knockout of host cell proteins for efficient biopharmaceutical manufacturing.

Authors: Marzluf, Jannis Peter; Klein, Jennifer; Kirchmeier, Daniela; Stärk, Alexander; Ziegner, Daniel; Gehrung, Markus; Rauh, Franziska; Rattay, Merle; Zehe, Christoph; Leroux, Ann-Cathrin

DOI: https://doi.org/10.3389/fbioe.2026.1750646

Paper

A comparison of SWATH-MS methods for measurement of residual host cell proteins in adeno-associated virus preparations.

Leibiger, Thomas M; Min, Lie; Lee, Kelvin H · 2025

Full title: A comparison of SWATH-MS methods for measurement of residual host cell proteins in adeno-associated virus preparations.

Authors: Leibiger, Thomas M; Min, Lie; Lee, Kelvin H

DOI: https://doi.org/10.3389/fbioe.2025.1579098

Paper

Host Cell Proteins in Biologics Manufacturing: The Good, the Bad, and the Ugly.

Kornecki, Martin; Mestmäcker, Fabian; Zobel-Roos, Steffen et al. · 2017

Full title: Host Cell Proteins in Biologics Manufacturing: The Good, the Bad, and the Ugly.

Authors: Kornecki, Martin; Mestmäcker, Fabian; Zobel-Roos, Steffen; Heikaus de Figueiredo, Laura; Schlüter, Hartmut; Strube, Jochen

DOI: https://doi.org/10.3390/antib6030013

Paper

A Combined Ultrafiltration/Diafiltration Step Facilitates the Purification of Cyanovirin-N From Transgenic Tobacco Extracts.

Opdensteinen, Patrick; Clodt, Juliana I; Müschen, Catherine R et al. · 2018

Full title: A Combined Ultrafiltration/Diafiltration Step Facilitates the Purification of Cyanovirin-N From Transgenic Tobacco Extracts.

Authors: Opdensteinen, Patrick; Clodt, Juliana I; Müschen, Catherine R; Filiz, Volkan; Buyel, Johannes F

DOI: https://doi.org/10.3389/fbioe.2018.00206

Paper

Residual host cell proteins: sources, properties, detection methods and data acquisition modes.

Yao, Yifan; Wen, Xuemei; Pan, Hongjuan et al. · 2025

Full title: Residual host cell proteins: sources, properties, detection methods and data acquisition modes.

Authors: Yao, Yifan; Wen, Xuemei; Pan, Hongjuan; Chen, Ziwei

DOI: https://doi.org/10.3389/fmicb.2025.1658366

Paper

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines

S. Michalik; F. Siegerist; R. Palankar et al. · 2021

Full title: Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines

DOI: https://doi.org/10.3324/haematol.2021.280154

Paper

Optimized Blanching Reduces the Host Cell Protein Content and Substantially Enhances the Recovery and Stability of Two Plant-Derived Malaria Vaccine Candidates.

Menzel, Stephan; Holland, Tanja; Boes, Alexander et al. · 2016

Full title: Optimized Blanching Reduces the Host Cell Protein Content and Substantially Enhances the Recovery and Stability of Two Plant-Derived Malaria Vaccine Candidates.

Authors: Menzel, Stephan; Holland, Tanja; Boes, Alexander; Spiegel, Holger; Bolzenius, Johanna; Fischer, Rainer; Buyel, Johannes F

DOI: https://doi.org/10.3389/fpls.2016.00159

Paper

Optimization of Sequence, Display, and Mode of Operation of IgG-Binding Peptide Ligands to Develop Robust, High-Capacity Affinity Adsorbents That Afford High IgG Product Quality.

Islam, Tuhidul; Naik, Amith D; Hashimoto, Yasuhiro et al. · 2019

Full title: Optimization of Sequence, Display, and Mode of Operation of IgG-Binding Peptide Ligands to Develop Robust, High-Capacity Affinity Adsorbents That Afford High IgG Product Quality.

Authors: Islam, Tuhidul; Naik, Amith D; Hashimoto, Yasuhiro; Menegatti, Stefano; Carbonell, Ruben G

DOI: https://doi.org/10.3390/ijms20010161

Paper

Site-Specific N-Glycosylation on the AAV8 Capsid Protein.

Aloor, Arya; Zhang, Junping; Gashash, Ebtesam A et al. · 2018

Full title: Site-Specific N-Glycosylation on the AAV8 Capsid Protein.

Authors: Aloor, Arya; Zhang, Junping; Gashash, Ebtesam A; Parameswaran, Aishwarya; Chrzanowski, Matthew; Ma, Cheng; Diao, Yong; Wang, Peng George; Xiao, Weidong

DOI: https://doi.org/10.3390/v10110644

Paper

In-Line Monitoring of Downstream Purification Processes for VSV Based SARS-CoV-2 Vaccine Using a Novel Technique.

Makovitzki, Arik; Jayson, Avital; Oren, Ziv et al. · 2021

Full title: In-Line Monitoring of Downstream Purification Processes for VSV Based SARS-CoV-2 Vaccine Using a Novel Technique.

DOI: https://doi.org/10.3390/biotech10040025

Paper

Nonwoven Ion-Exchange Membranes with High Protein Binding Capacity for Bioseparations.

Lemma, Solomon Mengistu; Boi, Cristiana; Carbonell, Ruben G · 2021

Full title: Nonwoven Ion-Exchange Membranes with High Protein Binding Capacity for Bioseparations.

Authors: Lemma, Solomon Mengistu; Boi, Cristiana; Carbonell, Ruben G

DOI: https://doi.org/10.3390/membranes11030181

Paper

Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery.

Lavoie, R Ashton; di Fazio, Alice; Blackburn, R Kevin et al. · 2019

Full title: Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery.

Authors: Lavoie, R Ashton; di Fazio, Alice; Blackburn, R Kevin; Goshe, Michael B; Carbonell, Ruben G; Menegatti, Stefano

DOI: https://doi.org/10.3390/ijms20071729

Paper

Multiplexed Competitive Screening of One-Bead-One-Component Combinatorial Libraries Using a ClonePix 2 Colony Sorter.

Lavoie, R Ashton; di Fazio, Alice; Carbonell, Ruben G et al. · 2019

Full title: Multiplexed Competitive Screening of One-Bead-One-Component Combinatorial Libraries Using a ClonePix 2 Colony Sorter.

Authors: Lavoie, R Ashton; di Fazio, Alice; Carbonell, Ruben G; Menegatti, Stefano

DOI: https://doi.org/10.3390/ijms20205119

Paper

Applying UHPLC-HRAM MS/MS Method to Assess Host Cell Protein Clearance during the Purification Process Development of Therapeutic mAbs.

Kiyonami, Reiko; Melani, Rafael; Chen, Ying et al. · 2024

Full title: Applying UHPLC-HRAM MS/MS Method to Assess Host Cell Protein Clearance during the Purification Process Development of Therapeutic mAbs.

Authors: Kiyonami, Reiko; Melani, Rafael; Chen, Ying; Leon, A I De; Du, Min

DOI: https://doi.org/10.3390/ijms25179687

Paper

A Single-Step Method for Harvesting Influenza Viral Particles from MDCK Cell Culture Supernatant with High Yield and Effective Impurity Removal.

Liu, Sixu; Li, Jingqi; Cheng, Qingtian et al. · 2024

Full title: A Single-Step Method for Harvesting Influenza Viral Particles from MDCK Cell Culture Supernatant with High Yield and Effective Impurity Removal.

Authors: Liu, Sixu; Li, Jingqi; Cheng, Qingtian; Duan, Kangyi; Wang, Zhan; Yan, Shuang; Tian, Shuaishuai; Wang, Hairui; Wu, Shaobin; Lei, Xinkui; Yang, Yu; Ma, Ningning

DOI: https://doi.org/10.3390/v16050768

Paper

Scout-Triggered Multiple Reaction Monitoring Enables Robust Quantification of Host Cell Proteins Across Bioprocess Matrices.

Flecheux, Julie; Bardet, Chloé; Herment, Laura et al. · 2026

Full title: Scout-Triggered Multiple Reaction Monitoring Enables Robust Quantification of Host Cell Proteins Across Bioprocess Matrices.

Authors: Flecheux, Julie; Bardet, Chloé; Herment, Laura; Fortin, Tanguy; Lemoine, Jérôme

DOI: https://doi.org/10.3390/proteomes14010009

Paper

Automated, Quantitative Capillary Western Blots to Analyze Host Cell Proteins in COVID-19 Vaccine Produced in Vero Cell Line.

Gillespie, Paul F; Wang, Yanjie; Yin, Kuo et al. · 2024

Full title: Automated, Quantitative Capillary Western Blots to Analyze Host Cell Proteins in COVID-19 Vaccine Produced in Vero Cell Line.

DOI: https://doi.org/10.3390/vaccines12121373

Paper

A Four-Step Purification Process for Gag VLPs: From Culture Supernatant to High-Purity Lyophilized Particles.

González-Domínguez, Irene; Lorenzo, Elianet; Bernier, Alice et al. · 2021

Full title: A Four-Step Purification Process for Gag VLPs: From Culture Supernatant to High-Purity Lyophilized Particles.

Authors: González-Domínguez, Irene; Lorenzo, Elianet; Bernier, Alice; Cervera, Laura; Gòdia, Francesc; Kamen, Amine

DOI: https://doi.org/10.3390/vaccines9101154

Paper

Label-Free Quantitative Proteomics Analysis of COVID-19 Vaccines by Nano LC-HRMS.

Zhao, Hengzhi; Li, Wendong; Liu, Jingjing et al. · 2024

Full title: Label-Free Quantitative Proteomics Analysis of COVID-19 Vaccines by Nano LC-HRMS.

Authors: Zhao, Hengzhi; Li, Wendong; Liu, Jingjing; Li, Xiao; Ji, Hong; Hu, Mo; Li, Min

DOI: https://doi.org/10.3390/vaccines12091055

Paper

Novel Standard Substance from Primary Hamster Kidney Cells for Quality Control of Human Rabies Vaccines in China.

Shi, Leitai; Li, Jia; Wu, Xiaohong et al. · 2025

Full title: Novel Standard Substance from Primary Hamster Kidney Cells for Quality Control of Human Rabies Vaccines in China.

Authors: Shi, Leitai; Li, Jia; Wu, Xiaohong; Cao, Shouchun; Wang, Yunpeng; Zhao, Danhua; Li, Yuhua

DOI: https://doi.org/10.3390/vaccines13020180

Paper

Quantitation of Host Cell Proteins by Capillary LC/IMS/MS/MS in Combination with Rapid Digestion on Immobilized Trypsin Column Under Native Conditions.

Tomioka, Ryota; Ogata, Kosuke; Ishihama, Yasushi · 2024

Full title: Quantitation of Host Cell Proteins by Capillary LC/IMS/MS/MS in Combination with Rapid Digestion on Immobilized Trypsin Column Under Native Conditions.

Authors: Tomioka, Ryota; Ogata, Kosuke; Ishihama, Yasushi

DOI: https://doi.org/10.5702/massspectrometry.A0152

Thesis

Prevention is better than cure : non-targeted and sensitive screening of impurities in plasma-derived immunoglobulin with mass spectrometry-based proteomics

Limonier, Franck · 2019

Full title: Prevention is better than cure : non-targeted and sensitive screening of impurities in plasma-derived immunoglobulin with mass spectrometry-based proteomics

Authors: Limonier, Franck

DOI: https://biblio.ugent.be/publication/8593810

Thesis

Complex binding and elution behavior of therapeutic proteins under column overloading conditions

Seelinger, Felix · 2023

Full title: Complex binding and elution behavior of therapeutic proteins under column overloading conditions

Authors: Seelinger, Felix

DOI: https://archiv.ub.uni-heidelberg.de/volltextserver/32857/1/SeelingerDissertation.pdf

Thesis

Mechanistic modeling and scale-up investigation of a preparative two-column purification step for a biopharmaceutical polypeptide

Koch, Jonas Martin Maria · 2024

Full title: Mechanistic modeling and scale-up investigation of a preparative two-column purification step for a biopharmaceutical polypeptide

Authors: Koch, Jonas Martin Maria

DOI: https://archiv.ub.uni-heidelberg.de/volltextserver/34272/1/Dissertation_Koch_Jonas.pdf

Thesis

Untersuchung systematischer Zusammenhänge zwischen Chromatographie-Gel Eigenschaften und Antikörper-spezifischen Verunreinigungen in der Säulenchromatographie

Wittkopp, Felix · 2018

Full title: Untersuchung systematischer Zusammenhänge zwischen Chromatographie-Gel Eigenschaften und Antikörper-spezifischen Verunreinigungen in der Säulenchromatographie

Authors: Wittkopp, Felix

DOI: https://archiv.ub.uni-heidelberg.de/volltextserver/25163/1/Dissertation%20Felix%20Wittkopp%20heiDOC.pdf

Thesis

Separation and characterization of antibody-based protein variants in column chromatography for the production of biopharmaceuticals

Sanchez Reyes, Gabriela · 2022

Full title: Separation and characterization of antibody-based protein variants in column chromatography for the production of biopharmaceuticals

Authors: Sanchez Reyes, Gabriela

DOI: https://archiv.ub.uni-heidelberg.de/volltextserver/30762/1/Dissertation_Sanchez%20Reyes%20Gabriela_FINAL.pdf

Thesis

Production of biosimilar trastuzumab in plants: Expression, purification, and analysis of function

Grohs, Brittany Marie · 2020

Full title: Production of biosimilar trastuzumab in plants: Expression, purification, and analysis of function

Authors: Grohs, Brittany Marie

DOI: https://hdl.handle.net/10214/21780

Thesis

Magnetic bead-based isolation of biological therapeutic modalities

Brechmann, Nils A. · 2022

Full title: Magnetic bead-based isolation of biological therapeutic modalities

Authors: Brechmann, Nils A.

DOI: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-321336

Thesis

Strategies for facilitated protein recovery after recombinant production in Escherichia coli

Hedhammar, My · 2005

Full title: Strategies for facilitated protein recovery after recombinant production in Escherichia coli

Authors: Hedhammar, My

DOI: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-471

Thesis

Calcium-dependent Affinity Domains for the Purification of Antibodies and Antibody Fragments

Scheffel, Julia · 2022

Full title: Calcium-dependent Affinity Domains for the Purification of Antibodies and Antibody Fragments

Authors: Scheffel, Julia

DOI: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-309572

Thesis

Engineering Proteinaceous Ligands for Improved Performance in Affinity Chromatography Applications

Gülich, Susanne · 2002

Full title: Engineering Proteinaceous Ligands for Improved Performance in Affinity Chromatography Applications

Authors: Gülich, Susanne

DOI: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3327

Thesis

Analysis of noncovalent and covalent protein-ligand complexes by electrospray ionisation mass spectrometry

Sundqvist, Gustav · 2008

Full title: Analysis of noncovalent and covalent protein-ligand complexes by electrospray ionisation mass spectrometry

Authors: Sundqvist, Gustav

DOI: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4728

Thesis

Solid Substrate for High Throughput Protein Chromatography

Alvarez, Adrian · 2018

Full title: Solid Substrate for High Throughput Protein Chromatography

Authors: Alvarez, Adrian

DOI: https://escholarship.org/uc/item/7hd470qf

Thesis

Process development for downstream processing of human growth hormone and its antagonist

Zheng, Yizhou · 1994

Full title: Process development for downstream processing of human growth hormone and its antagonist

Authors: Zheng, Yizhou

DOI: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1174330360

Thesis

Příprava a charakterizace průtokových reaktoru pro validaci biologických léčiv

Kordík, Jakub · 2025

Full title: Příprava a charakterizace průtokových reaktoru pro validaci biologických léčiv

Authors: Kordík, Jakub

DOI: http://hdl.handle.net/20.500.11956/198905

Thesis

Development of an integrated platform for the production of recombinant biopharmaceuticals

Segarra, Camille · 2013

Full title: Development of an integrated platform for the production of recombinant biopharmaceuticals

Authors: Segarra, Camille

DOI: https://etheses.whiterose.ac.uk/id/eprint/4147/1/Segarra_Thesis_PostCorrections.pdf

Thesis

Evaluating Improvements and Challenges in Affinity Chromatography for AAV Purification

Dachenko, Alexandra · 2023

Full title: Evaluating Improvements and Challenges in Affinity Chromatography for AAV Purification

Authors: Dachenko, Alexandra

DOI: https://jscholarship.library.jhu.edu/handle/1774.2/69011

Thesis

HARNESSING THE HIGH-AFFINITY INTERACTIONS BETWEEN SUPRAMOLECULAR POLYMERS AND PROTEINS FOR ANTIBODY SEPARATION AND LONG-ACTING RELEASE

Stern, David · 2022

Full title: HARNESSING THE HIGH-AFFINITY INTERACTIONS BETWEEN SUPRAMOLECULAR POLYMERS AND PROTEINS FOR ANTIBODY SEPARATION AND LONG-ACTING RELEASE

Authors: Stern, David

DOI: http://jhir.library.jhu.edu/handle/1774.2/67519

Thesis

Bioprocess development: extraction and purification of human serum albumin from transgenic rice

Sheshukova, Kseniya Alekseyevna · 2019

Full title: Bioprocess development: extraction and purification of human serum albumin from transgenic rice

Authors: Sheshukova, Kseniya Alekseyevna

DOI: http://hdl.handle.net/2097/39681

Thesis

Design and Control of Integrated Continuous Processes for the Purification of Biopharmaceuticals

Gomis Fons, Joaquin · 2022

Full title: Design and Control of Integrated Continuous Processes for the Purification of Biopharmaceuticals

Authors: Gomis Fons, Joaquin

DOI: https://lup.lub.lu.se/record/7de201f7-6a1b-4772-b3d1-e5d221e9515c

Thesis

Modeling and Optimization of Reversed-Phase Chromatography : Effects of Modulators and Temperature

Arkell, Karolina · 2017

Full title: Modeling and Optimization of Reversed-Phase Chromatography : Effects of Modulators and Temperature

Authors: Arkell, Karolina

DOI: https://lup.lub.lu.se/record/145c9644-a818-4f5e-ab07-9a79be1655bc

Thesis

Developing integrated downstream processes for next-generation biologics

Löfgren, Anton · 2020

Full title: Developing integrated downstream processes for next-generation biologics

Authors: Löfgren, Anton

DOI: https://lup.lub.lu.se/record/31772706-1f08-4ef5-b61e-db17291cd22b

Thesis

Antibody-Fragments: Protein Engineering, Process Development, and their Application as Affinity Ligands

Sakhnini, Laila · 2019

Full title: Antibody-Fragments: Protein Engineering, Process Development, and their Application as Affinity Ligands

Authors: Sakhnini, Laila

DOI: https://lup.lub.lu.se/record/3156874d-3d8d-41ad-bd7a-6be6ffb8e037

Thesis

Development and application of two-dimensional separation systems coupled to mass spectrometry incorporating capillary electrophoresis

Jooß, Kevin · 2019

Full title: Development and application of two-dimensional separation systems coupled to mass spectrometry incorporating capillary electrophoresis

Authors: Jooß, Kevin

DOI: https://mediatum.ub.tum.de/1469960

Thesis

Monoclonal Antibody Production and Purification

McMahon, Hope E; Schwartz, Jessica W; Ray, Shritama · 2023

Full title: Monoclonal Antibody Production and Purification

Authors: McMahon, Hope E; Schwartz, Jessica W; Ray, Shritama

DOI: https://repository.upenn.edu/handle/20.500.14332/5925

Thesis

Downstream purification and analysis of the recombinant human myelin basic protein produced in the milk of transgenic cows : a thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Chemistry, Massey University (Palmerston North) New Zealand.

Al-Ghobashy, Medhat Ahmed Abdel-Hamid · 2010

Authors: Al-Ghobashy, Medhat Ahmed Abdel-Hamid

DOI: http://hdl.handle.net/10179/1186

Thesis

Statistical issues on mass spectrometry-based protein identification and quantitation

Liu, Shangbin · 2024

Full title: Statistical issues on mass spectrometry-based protein identification and quantitation

Authors: Liu, Shangbin

DOI: https://openscholar.uga.edu/record/19545/files/liu_shangbin_200812_phd.pdf

Thesis

Membrane adsorbers and novel affinity peptides for recombinant protein purification

Weaver, Justin, author; Wickramasinghe, S. Ranil, advisor; Qian, Xianghong, committee member et al. · 2015

Full title: Membrane adsorbers and novel affinity peptides for recombinant protein purification

Authors: Weaver, Justin, author; Wickramasinghe, S. Ranil, advisor; Qian, Xianghong, committee member; Carlson, Jon O., committee member; Bailey, Travis S., committee member

DOI: http://hdl.handle.net/10217/166862

Thesis

Multidimensional liquid chromatographic separations for proteomics

Burgess, Karl · 2008

Full title: Multidimensional liquid chromatographic separations for proteomics

Authors: Burgess, Karl

DOI: https://theses.gla.ac.uk/322/1/2008burgessphd.pdf

Thesis

Purification of recombinant biopharmaceuticals by preparative electrophoresis

Catzel, Dallia · 2006

Full title: Purification of recombinant biopharmaceuticals by preparative electrophoresis

Authors: Catzel, Dallia

DOI: http://hdl.handle.net/1959.4/68595

Thesis

Downstream processing of recombinant and endogenous proteins from livestock milk

Degener, Arthur W. Jr. · 2014

Full title: Downstream processing of recombinant and endogenous proteins from livestock milk

Authors: Degener, Arthur W. Jr.

DOI: http://hdl.handle.net/10919/27183

Thesis

Identification of the factors impacting immunosorbent performance

Subramanian, Anuradha · 2014

Full title: Identification of the factors impacting immunosorbent performance

Authors: Subramanian, Anuradha

DOI: http://hdl.handle.net/10919/38450

Thesis

Purification of the major envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions

Matanin, Brad Matthew · 2014

Full title: Purification of the major envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions

Authors: Matanin, Brad Matthew

DOI: http://hdl.handle.net/10919/33194

Thesis

Purification of an acidic recombinant protein from transgenic tobacco

Holler, Christopher J. · 2014

Full title: Purification of an acidic recombinant protein from transgenic tobacco

Authors: Holler, Christopher J.

DOI: http://hdl.handle.net/10919/32379

Thesis

Separation of Recombinant β-Glucuronidase from Transgenic Tobacco by Aqueous Two-Phase Extraction

Ross, Kristin Coby · 2014

Full title: Separation of Recombinant β-Glucuronidase from Transgenic Tobacco by Aqueous Two-Phase Extraction

Authors: Ross, Kristin Coby

DOI: http://hdl.handle.net/10919/43471

Thesis

Evaluation of Phenomena that Determine the Performance of Immunoaffinity, Peptide-Based and Ion Exchange Affinity Sorbents

Sines, Brian James · 2014

Full title: Evaluation of Phenomena that Determine the Performance of Immunoaffinity, Peptide-Based and Ion Exchange Affinity Sorbents

Authors: Sines, Brian James

DOI: http://hdl.handle.net/10919/29841

Thesis

Design of anion exchange cellulose hydrogel for large proteins

Kumar, Guneet · 2014

Full title: Design of anion exchange cellulose hydrogel for large proteins

Authors: Kumar, Guneet

DOI: http://hdl.handle.net/10919/38250

Thesis

Antibody Purification from Tobacco by Protein A Affinity Chromatography

Hey, Carolyn McKenzie · 2014

Full title: Antibody Purification from Tobacco by Protein A Affinity Chromatography

Authors: Hey, Carolyn McKenzie

DOI: http://hdl.handle.net/10919/42645

Thesis

Purification, formulation, and functionalization of HIV-1-based virus-like particles for vaccine and therapeutic applications

Lorenzo Romero, Elianet · 2024

Full title: Purification, formulation, and functionalization of HIV-1-based virus-like particles for vaccine and therapeutic applications

Authors: Lorenzo Romero, Elianet

DOI: http://hdl.handle.net/10803/692884

Thesis

Developing and Establishing a Platform for the Automation of Monoclonal Antibody Drug Analysis

Federico, Joseph Vincent · 2019

Full title: Developing and Establishing a Platform for the Automation of Monoclonal Antibody Drug Analysis

Authors: Federico, Joseph Vincent

DOI: http://jhir.library.jhu.edu/handle/1774.2/60162

Thesis

Improving Protein Detection Confidence Using SWATH-Mass Spectrometry with Large Peptide Reference Libraries

Wu, JX; Pascovici, D; Ignjatovic, V et al. · 2021

Full title: Improving Protein Detection Confidence Using SWATH-Mass Spectrometry with Large Peptide Reference Libraries

Authors: Wu, JX; Pascovici, D; Ignjatovic, V; Song, X; Krisp, C; Molloy, MP

DOI: https://hdl.handle.net/11343/293523

Thesis

Measuring Hordein (Gluten) in Beer - A Comparison of ELISA and Mass Spectrometry

Tanner, GJ; Colgrave, ML; Blundell, MJ et al. · 2019

Full title: Measuring Hordein (Gluten) in Beer - A Comparison of ELISA and Mass Spectrometry

Authors: Tanner, GJ; Colgrave, ML; Blundell, MJ; Goswami, HP; Howitt, CA

DOI: https://hdl.handle.net/11343/224112

Thesis

Application of mechanistic modelling in membrane and fiber chromatography for purification of biotherapeutics — A review

Qu, Y; Baker, I; Black, J et al. · 2024

Full title: Application of mechanistic modelling in membrane and fiber chromatography for purification of biotherapeutics — A review

Authors: Qu, Y; Baker, I; Black, J; Fabri, L; Gras, SL; Lenhoff, AM; Kentish, SE

DOI: https://hdl.handle.net/11343/340258

Thesis

Protein A chromatography in monoclonal antibody purification

Curtis, Michael · 2018

Full title: Protein A chromatography in monoclonal antibody purification

Authors: Curtis, Michael

DOI: http://hdl.handle.net/2097/39284

Thesis

Primary Recovery of Biologically Active Compounds Using Macroporous Monolithic Hydrogels

Hanora, Amro · 2005

Full title: Primary Recovery of Biologically Active Compounds Using Macroporous Monolithic Hydrogels

Authors: Hanora, Amro

DOI: https://lup.lub.lu.se/record/545390

Thesis

Model Calibration and Optimization of a Protein Purification Process

Al-Kaisy, Sadiq · 2015

Full title: Model Calibration and Optimization of a Protein Purification Process

Authors: Al-Kaisy, Sadiq

DOI: http://lup.lub.lu.se/student-papers/record/5470040

Thesis

Tools for digital twins in continuous downstream processing

Tallvod, Simon · 2023

Full title: Tools for digital twins in continuous downstream processing

Authors: Tallvod, Simon

DOI: https://lup.lub.lu.se/record/191aa4b8-990d-47f1-a248-68bfb57ff330

Thesis

Modeling & Simulation of Affinity Chromatography & Investigation of CaptureSMB

Bogren, Dennis · 2018

Full title: Modeling & Simulation of Affinity Chromatography & Investigation of CaptureSMB

Authors: Bogren, Dennis

DOI: http://lup.lub.lu.se/student-papers/record/8951165

Thesis

Iterative Learning Control for Preparative Chromatography

Espinoza, Daniel · 2020

Full title: Iterative Learning Control for Preparative Chromatography

Authors: Espinoza, Daniel

DOI: http://lup.lub.lu.se/student-papers/record/9007394

Thesis

High throughput optimization of protein-A affinity chromatography for capture of humanized monoclonal antibody

Pakiman, Norazrina · 2012

Full title: High throughput optimization of protein-A affinity chromatography for capture of humanized monoclonal antibody

Authors: Pakiman, Norazrina

DOI: http://psasir.upm.edu.my/id/eprint/48480/1/FK%202012%20123R.pdf

Thesis

Development of dye affinity adsorbents for recovery of polyclonal anti-hepatitis B core antigen immunoglobulin G

Wongchuphan, Rattana · 2010

Full title: Development of dye affinity adsorbents for recovery of polyclonal anti-hepatitis B core antigen immunoglobulin G

Authors: Wongchuphan, Rattana

DOI: http://psasir.upm.edu.my/id/eprint/41141/1/FK%202010%2076R.pdf

Thesis

CHARACTERIZATION AND QUANTITATION TECHNIQUES FOR IMMUNOGLOBULINS AND BIOTHERAPEUTIC DRUGS WITH MASS SPECTROMETRY

Fletcher, Tyler · 2024

Full title: CHARACTERIZATION AND QUANTITATION TECHNIQUES FOR IMMUNOGLOBULINS AND BIOTHERAPEUTIC DRUGS WITH MASS SPECTROMETRY

Authors: Fletcher, Tyler

DOI: https://openscholar.uga.edu/record/4525/files/FletcherTylerPHD.pdf

Thesis

Purification and characterization of polyclonal anti-hepatitis B core antigen immunoglobulin G from rabbit serum

Sayed Hitam, Sharifah Mariam · 2016

Full title: Purification and characterization of polyclonal anti-hepatitis B core antigen immunoglobulin G from rabbit serum

Authors: Sayed Hitam, Sharifah Mariam

DOI: http://psasir.upm.edu.my/id/eprint/69717/1/IB%202016%207%20IR.pdf

Thesis

Production and purification of nipah virus glycoprotein in Spodoptera frugiperda 9 (J.E. Smith) insect cell

Sunhare, Raksha · 2013

Full title: Production and purification of nipah virus glycoprotein in Spodoptera frugiperda 9 (J.E. Smith) insect cell

Authors: Sunhare, Raksha

DOI: http://psasir.upm.edu.my/id/eprint/40015/1/IB%202013%209.pdf

Thesis

Direct Recovery of Recombinant Hepatitis B Core Antigen from Unclarified Escherichia Coli Feedstock Using Expanded Bed Adsorption Chromatography

Ng, Michelle Yeen Tan · 2008

Full title: Direct Recovery of Recombinant Hepatitis B Core Antigen from Unclarified Escherichia Coli Feedstock Using Expanded Bed Adsorption Chromatography

Authors: Ng, Michelle Yeen Tan

DOI: http://psasir.upm.edu.my/id/eprint/5392/1/IB_2008_5.pdf

Thesis

Chromatographic Purification of Recombinant Nucleocapsid Protein of Nipah Virus from Escherichia Coli Homogenate

Chong, Fui Chin · 2010

Full title: Chromatographic Purification of Recombinant Nucleocapsid Protein of Nipah Virus from Escherichia Coli Homogenate

Authors: Chong, Fui Chin

DOI: http://psasir.upm.edu.my/id/eprint/7849/1/ABS_%3D%3D%3D%3D__FK_2010_3.pdf

Thesis

Development Of An Integrated Recovery Process For Recombinant Hepatitis B Core Antigen

Ho, Chin Woi · 2007

Full title: Development Of An Integrated Recovery Process For Recombinant Hepatitis B Core Antigen

Authors: Ho, Chin Woi

DOI: http://psasir.upm.edu.my/id/eprint/5210/1/FK_2007_27.pdf

Thesis

Partitioning behaviours and selective recovery of therapeutic protein in aqueous two-phase system

Chow, Yin Hui · 2016

Full title: Partitioning behaviours and selective recovery of therapeutic protein in aqueous two-phase system

Authors: Chow, Yin Hui

DOI: http://psasir.upm.edu.my/id/eprint/70219/1/FK%202016%2021%20T%20IR.pdf

Thesis

The Influence Of M13 Bacteriophage On Protein Adsorption Using Anion Exchange Expanded Bed Chromatography

Mohamed @ Mahmood, Masniza · 2006

Full title: The Influence Of M13 Bacteriophage On Protein Adsorption Using Anion Exchange Expanded Bed Chromatography

Authors: Mohamed @ Mahmood, Masniza

DOI: http://psasir.upm.edu.my/id/eprint/5162/1/FK_2006_108a.pdf

Thesis

Data concerning the chromatographic isolation of bovine IgG from milk- and colostral whey

Heidebrecht, Hans-Jürgen; Kainz, Bernadette; Schopf, Roland et al. · 2018

Full title: Data concerning the chromatographic isolation of bovine IgG from milk- and colostral whey

Authors: Heidebrecht, Hans-Jürgen; Kainz, Bernadette; Schopf, Roland; Godl, Klaus; Karcier, Züleyha; Kulozik, Ulrich; Förster, Beatrix

DOI: https://dspace.library.uu.nl/handle/1874/384260

Thesis

Isolation of biofunctional bovine immunoglobulin G from milk- and colostral whey with mixed-mode chromatography at lab and pilot scale

Heidebrecht, Hans-Jürgen; Kainz, Bernadette; Schopf, Roland et al. · 2018

Full title: Isolation of biofunctional bovine immunoglobulin G from milk- and colostral whey with mixed-mode chromatography at lab and pilot scale

Authors: Heidebrecht, Hans-Jürgen; Kainz, Bernadette; Schopf, Roland; Godl, Klaus; Karcier, Züleyha; Kulozik, Ulrich; Förster, Beatrix

DOI: https://dspace.library.uu.nl/handle/1874/384261

Thesis

On-demand manufacturing of clinical-quality biopharmaceuticals

Crowell, Laura E; Lu, Amos E; Love, Kerry R et al. · 2021

Full title: On-demand manufacturing of clinical-quality biopharmaceuticals

DOI: https://hdl.handle.net/1721.1/134719

Thesis

Rapid optimization of processes for the integrated purification of biopharmaceuticals

Crowell, Laura E; Rodriguez, Sergio A; Love, Kerry R et al. · 2021

Full title: Rapid optimization of processes for the integrated purification of biopharmaceuticals

Authors: Crowell, Laura E; Rodriguez, Sergio A; Love, Kerry R; Cramer, Steven M; Love, J Christopher

DOI: https://hdl.handle.net/1721.1/135519

Thesis

Minimal purification method enables developability assessment of recombinant proteins

Rodriguez‐Aponte, Sergio A; Naranjo, Christopher A; Johnston, Ryan S et al. · 2025

Full title: Minimal purification method enables developability assessment of recombinant proteins

DOI: https://hdl.handle.net/1721.1/163511

Thesis

Understanding electrostatic interaction on strong cation-exchanger via co-ion valency effects

Essert, GM; de Souza, JP; Schwaminger, SP et al. · 2024

Full title: Understanding electrostatic interaction on strong cation-exchanger via co-ion valency effects

Authors: Essert, GM; de Souza, JP; Schwaminger, SP; Bazant, MZ; Berensmeier, S

DOI: https://hdl.handle.net/1721.1/157408

Thesis

Large-Scale Purification and Characterization of Recombinant Receptor-Binding Domain (RBD) of SARS-CoV-2 Spike Protein Expressed in Yeast

Nagar, Gaurav; Jain, Siddharth; Rajurkar, Meghraj et al. · 2023

Full title: Large-Scale Purification and Characterization of Recombinant Receptor-Binding Domain (RBD) of SARS-CoV-2 Spike Protein Expressed in Yeast

DOI: https://hdl.handle.net/1721.1/152537

Thesis

Development of a platform process for the production and purification of single‐domain antibodies

Crowell, Laura E; Goodwine, Chaz; Holt, Carla S et al. · 2021

Full title: Development of a platform process for the production and purification of single‐domain antibodies

DOI: https://hdl.handle.net/1721.1/135524

Thesis

Purification of recombinant proteins

Rutt, George Clifford · 2008

Full title: Purification of recombinant proteins

Authors: Rutt, George Clifford

DOI: http://hdl.handle.net/1721.1/42614

Thesis

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Zhao, Lei; Whiteaker, Jeffrey R.; Pope, Matthew E. et al. · 2014

Full title: Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Authors: Zhao, Lei; Whiteaker, Jeffrey R.; Pope, Matthew E.; Kuhn, Eric; Jackson, Angela; Anderson, N. Leigh; Pearson, Terry W.; Paulovich, Amanda G.; Carr, Steven A

DOI: http://hdl.handle.net/1721.1/89657

Thesis

HIGH EFFICIENCY AFFINITY CAPTURE AND PURIFICATION OF IMMUNOGLOBULIN G USING MAGNETIC PARTICLES WITH BIOINSPIRIED PEPTIDE LIGANDS

Ou, Shih-Hao · 2019

Full title: HIGH EFFICIENCY AFFINITY CAPTURE AND PURIFICATION OF IMMUNOGLOBULIN G USING MAGNETIC PARTICLES WITH BIOINSPIRIED PEPTIDE LIGANDS

Authors: Ou, Shih-Hao

DOI: http://jhir.library.jhu.edu/handle/1774.2/61606

Thesis

Resin Lifetime and Aging Behavior in Protein A Chromatography

Gooley, Sarah · 2024

Full title: Resin Lifetime and Aging Behavior in Protein A Chromatography

Authors: Gooley, Sarah

DOI: https://jscholarship.library.jhu.edu/handle/1774.2/69585

Thesis

Insight into purification of monoclonal antibodies in industrial columns via studies of Protein A binding capacity by in situ ATR-FTIR spectroscopy

Beattie, james; Rowland-Jones, Ruth; Farys, Monika et al. · 2021

Full title: Insight into purification of monoclonal antibodies in industrial columns via studies of Protein A binding capacity by in situ ATR-FTIR spectroscopy

Authors: Beattie, james; Rowland-Jones, Ruth; Farys, Monika; Kucia-Tran, Richard; Kazarian, Sergei; Byrne, Bernadette

DOI: https://pubs.rsc.org/en/content/articlelanding/2021/AN/D1AN00985K

Thesis

Characterization of the diffusive properties of the affinity chromatography system of Human Immunoglobulin-G and Protein A Sepharose

Dutta, Rañjita · 1991

Full title: Characterization of the diffusive properties of the affinity chromatography system of Human Immunoglobulin-G and Protein A Sepharose

Authors: Dutta, Rañjita

DOI: http://hdl.handle.net/1959.4/59597

Thesis

Monoclonal Antibody Expression and Novel Purification in Nicotiana benthamiana

Fulton, Andrew Dale · 2014

Full title: Monoclonal Antibody Expression and Novel Purification in Nicotiana benthamiana

Authors: Fulton, Andrew Dale

DOI: http://hdl.handle.net/10919/43361

Thesis

The development of a weak anion micro-capillary film for protein chromatography.

Kouyoumdjian, AJM; Lazar, RA; Slater, NKH · 2016

Full title: The development of a weak anion micro-capillary film for protein chromatography.

Authors: Kouyoumdjian, AJM; Lazar, RA; Slater, NKH

DOI: https://www.repository.cam.ac.uk/handle/1810/260967

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us-2009247735-a1

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