PaperStatistical Improvement of Batch Culture with Immobilized <i>Pichia pastoris</i> Cells for rPOXA 1B Laccase Production
Gouzy-Olmos, Michelle, Ch�ves-Tequia, Luis M., Rojas-Fajardo, María F., Morales-Álvarez, Edwin D., Rivera-Hoyos, Claudia M., Poutou‐Piñales, Raúl A., González-Neira, Eliana María, Reyes-Montaño, Edgar Antonio, Cardozo-Bernal, Ángela M., Gómez-Méndez, Luis David, Pedroza-Rodríguez, Aura M. · 2018 · American journal of biochemistry & biotechnology/American journal of biochemistry and biotechnology · DOI
Full title: Statistical Improvement of Batch Culture with Immobilized <i>Pichia pastoris</i> Cells for rPOXA 1B Laccase Production
Authors: Gouzy-Olmos, Michelle, Ch�ves-Tequia, Luis M., Rojas-Fajardo, María F., Morales-Álvarez, Edwin D., Rivera-Hoyos, Claudia M., Poutou‐Piñales, Raúl A., González-Neira, Eliana María, Reyes-Montaño, Edgar Antonio, Cardozo-Bernal, Ángela M., Gómez-Méndez, Luis David, Pedroza-Rodríguez, Aura M.
Year: 2018
Venue: American journal of biochemistry & biotechnology/American journal of biochemistry and biotechnology
PaperPreparation and functional characterization of human vascular endothelial growth factor-melittin fusion protein with analysis of the antitumor activity in vitro and in vivo
Wang, Dingding, Hu, Lili, Su, Manman, Wang, Ju, Xu, Tianmin · 2015 · International Journal of Oncology · DOI
Full title: Preparation and functional characterization of human vascular endothelial growth factor-melittin fusion protein with analysis of the antitumor activity in vitro and in vivo
PaperSecretion and expression dynamics of a GFP-tagged mucin-type fusion protein in high cell density &lt;i&gt;Pichia pastoris&lt;/i&gt; bioreactor cultivations
Sjöblom, Magnus, Lindberg, Linda, Holgersson, Jan, Rova, Ulrika · 2012 · Advances in Bioscience and Biotechnology · DOI
Full title: Secretion and expression dynamics of a GFP-tagged mucin-type fusion protein in high cell density &lt;i&gt;Pichia pastoris&lt;/i&gt; bioreactor cultivations
PaperHigh-level secretory expression, purification, and characterization of an anti-human Her II monoclonal antibody, trastuzumab, in the methylotrophic yeast &lt;i&gt;Pichia pastoris&lt;/i&gt;
Shibui, Tatsuro, Bando, Keisuke, Misawa, S. · 2013 · Advances in Bioscience and Biotechnology · DOI
Full title: High-level secretory expression, purification, and characterization of an anti-human Her II monoclonal antibody, trastuzumab, in the methylotrophic yeast &lt;i&gt;Pichia pastoris&lt;/i&gt;
Authors: Shibui, Tatsuro, Bando, Keisuke, Misawa, S.
PaperIntensification of Recombinant Laccase Production from Sago Bioethanol Liquid Waste and Evaluation of The Enzyme for Synthetic Dye Decolourisation
Suhaili, Nurashikin, Mamat, Farah Wahida, Sing, Ngieng Ngui, Husaini, Ahmad, Vincent, Micky, Adeni, Dayang Salwani Awang · 2022 · Arab Gulf Journal of Scientific Research · DOI
Full title: Intensification of Recombinant Laccase Production from Sago Bioethanol Liquid Waste and Evaluation of The Enzyme for Synthetic Dye Decolourisation
PaperTalaromyces marneffei Cu, Zn Superoxide Dismutase Recombinant Protein Expression in Pichia pastoris, Enzymatic Activity and Its Resistance to Oxidative Stress
S. Khanthawong, K. Wongsawan, R. Pratanaphon, N. Vanittanakom · 2024 · Asian Health, Science and Technology Reports · DOI
Full title: Talaromyces marneffei Cu, Zn Superoxide Dismutase Recombinant Protein Expression in Pichia pastoris, Enzymatic Activity and Its Resistance to Oxidative Stress
Authors: S. Khanthawong, K. Wongsawan, R. Pratanaphon, N. Vanittanakom
Year: 2024
Venue: Asian Health, Science and Technology Reports
PaperPeer Review #1 of "Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli (v0.1)"
Full title: Peer Review #1 of "Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli (v0.1)"
PaperExpression of human serum albumin in Pichia pastoris protease-deficient host and conjugation with gadolinium-diethylenetriamine pentaacetate for application as a contrast agent
Gaffar, Shabarni, Anggraeni, Rina, Novianti, Mia Tria, Ishmayana, Safri, Soedjanaatmadja, Ukun M.S. · 2021 · Journal of Applied Pharmaceutical Science · DOI
Full title: Expression of human serum albumin in Pichia pastoris protease-deficient host and conjugation with gadolinium-diethylenetriamine pentaacetate for application as a contrast agent
Authors: Gaffar, Shabarni, Anggraeni, Rina, Novianti, Mia Tria, Ishmayana, Safri, Soedjanaatmadja, Ukun M.S.
PaperInvestigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfolded proteins using chemostat cultures
Hesketh, Andrew, Castrillo, Juan I., Sawyer, Trevor, Archer, David B., Oliver, Stephen G. · 2013 · Applied Microbiology and Biotechnology · DOI
Full title: Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfolded proteins using chemostat cultures
Authors: Hesketh, Andrew, Castrillo, Juan I., Sawyer, Trevor, Archer, David B., Oliver, Stephen G.
PaperEffects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter
Looser, Verena, Lüthy, Dominik, Straumann, Marcel, Hecht, Katrin, Melzoch, Karel, Kovar, Karin · 2017 · Applied Microbiology and Biotechnology · DOI
Full title: Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter
PaperHigh-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors
Jankowski, Nina, Koschorreck, Katja, Urlacher, Vlada B. · 2020 · Applied Microbiology and Biotechnology · DOI
Full title: High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors
Authors: Jankowski, Nina, Koschorreck, Katja, Urlacher, Vlada B.
PaperImpact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-state-maintained continuous cultures
Korpys-Woźniak, Paulina, Kubiak, Piotr, Białas, W., Celińska, Ewelina · 2020 · Applied Microbiology and Biotechnology · DOI
Full title: Impact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-state-maintained continuous cultures
Authors: Korpys-Woźniak, Paulina, Kubiak, Piotr, Białas, W., Celińska, Ewelina
PaperHyperosmolarity adversely impacts recombinant protein synthesis by Yarrowia lipolytica—molecular background revealed by quantitative proteomics
Kubiak-Szymendera, Monika, Skupień-Rabian, Bożena, Jankowska, Urszula, Celińska, Ewelina · 2021 · Applied Microbiology and Biotechnology · DOI
Full title: Hyperosmolarity adversely impacts recombinant protein synthesis by Yarrowia lipolytica—molecular background revealed by quantitative proteomics
PaperHigh-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
Blanchard, Véronique, Gadkari, Rupali A., George, A. V. E., Roy, Satarupa Sinha, Gerwig, Gerrit J., Leeflang, Bas R., Dighe, Rajan R., Boelens, Rolf, Kamerling, Johannis P. · 2008 · Glycoconjugate Journal · DOI
Full title: High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
Authors: Blanchard, Véronique, Gadkari, Rupali A., George, A. V. E., Roy, Satarupa Sinha, Gerwig, Gerrit J., Leeflang, Bas R., Dighe, Rajan R., Boelens, Rolf, Kamerling, Johannis P.
Full title: Cloning and expression of Saccharomyces cerevisiae SUC2 gene in yeast platform and characterization of recombinant enzyme biochemical properties.
PaperMethanol addition after glucose depletion improves rPOXA 1B production under the pGap in P. pastoris X33: breaking the habit
Ardila-Leal, Leidy D., Poutou‐Piñales, Raúl A., Morales-Álvarez, Edwin D., Rivera-Hoyos, Claudia M., Pedroza-Rodríguez, Aura M., Quevedo-Hidalgo, Balkys, Pérez, Alejandro · 2021 · SN Applied Sciences · DOI
Full title: Methanol addition after glucose depletion improves rPOXA 1B production under the pGap in P. pastoris X33: breaking the habit
Authors: Ardila-Leal, Leidy D., Poutou‐Piñales, Raúl A., Morales-Álvarez, Edwin D., Rivera-Hoyos, Claudia M., Pedroza-Rodríguez, Aura M., Quevedo-Hidalgo, Balkys, Pérez, Alejandro
PaperFunctional characterisation of a recombinant xylanase from<i>Pichia pastoris</i>and effect of the enzyme on nutrient digestibility in weaned pigs
He, Jun, Jia, Yin, Wang, Li, Yu, Bing, Chen, Daiwen · 2010 · British Journal Of Nutrition · DOI
Full title: Functional characterisation of a recombinant xylanase from<i>Pichia pastoris</i>and effect of the enzyme on nutrient digestibility in weaned pigs
PaperHigh-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin
Chen, Ximing, Li, Juan, Sun, Haili, Li, Shi-Weng, Chen, Tuo, Liu, Guangxiu, Dyson, Paul · 2017 · Scientific Reports · DOI
Full title: High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin
Authors: Chen, Ximing, Li, Juan, Sun, Haili, Li, Shi-Weng, Chen, Tuo, Liu, Guangxiu, Dyson, Paul
PaperIdentification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Yu, Xiao‐Wei, Sun, Weihong, Wang, Ying-Zheng, Xu, Yan · 2017 · Scientific Reports · DOI
Full title: Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Authors: Yu, Xiao‐Wei, Sun, Weihong, Wang, Ying-Zheng, Xu, Yan
PaperMulti-omics integrative analysis with genome-scale metabolic model simulation reveals global cellular adaptation of Aspergillus niger under industrial enzyme production condition
Full title: Multi-omics integrative analysis with genome-scale metabolic model simulation reveals global cellular adaptation of Aspergillus niger under industrial enzyme production condition
PaperEnzymatic activity of a recombinant β-1,4-endoglucanase from the Cotton Boll Weevil (Anthonomus grandis) aiming second generation ethanol production
Ibarra, Liz Nathalia, Alves, Ana Elizabeth Oliveira de Araújo, Antonino, José Dijair, Prado, Guilherme Souza, Pinto, Clidia Eduarda Moreira, Soccol, Carlos Ricardo, Vasconcelos, Érico Augusto Rosas de, Grossi‐de‐Sá, Maria Fátima · 2019 · Scientific Reports · DOI
Full title: Enzymatic activity of a recombinant β-1,4-endoglucanase from the Cotton Boll Weevil (Anthonomus grandis) aiming second generation ethanol production
Authors: Ibarra, Liz Nathalia, Alves, Ana Elizabeth Oliveira de Araújo, Antonino, José Dijair, Prado, Guilherme Souza, Pinto, Clidia Eduarda Moreira, Soccol, Carlos Ricardo, Vasconcelos, Érico Augusto Rosas de, Grossi‐de‐Sá, Maria Fátima
PaperPredicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden
Wollborn, David, Munkler, Lara Pauline, Horstmann, Rebekka, Germer, Andrea, Blank, Lars M., Büchs, Jochen · 2022 · Scientific Reports · DOI
Full title: Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden
Authors: Wollborn, David, Munkler, Lara Pauline, Horstmann, Rebekka, Germer, Andrea, Blank, Lars M., Büchs, Jochen
PaperProcess development for an effective COVID-19 vaccine candidate harboring recombinant SARS-CoV-2 delta plus receptor binding domain produced by Pichia pastoris
Full title: Process development for an effective COVID-19 vaccine candidate harboring recombinant SARS-CoV-2 delta plus receptor binding domain produced by Pichia pastoris
PaperPathway elucidation of bioactive rhamnosylated ginsenosides in Panax ginseng and their de novo high-level production by engineered Saccharomyces cerevisiae
Full title: Pathway elucidation of bioactive rhamnosylated ginsenosides in Panax ginseng and their de novo high-level production by engineered Saccharomyces cerevisiae
Full title: Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification.
PaperScaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement
Full title: Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement
PaperHigh-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation
Full title: High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation
Full title: Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris
PaperMetabolic flux analysis: a comprehensive review on sample preparation, analytical techniques, data analysis, computational modelling, and main application areas
Falco, Bruna de, Giannino, Francesco, Cartenì, Fabrizio, Mazzoleni, Stefano, Kim, Dong‐Hyun · 2022 · RSC Advances · DOI
Full title: Metabolic flux analysis: a comprehensive review on sample preparation, analytical techniques, data analysis, computational modelling, and main application areas
Authors: Falco, Bruna de, Giannino, Francesco, Cartenì, Fabrizio, Mazzoleni, Stefano, Kim, Dong‐Hyun
PaperBiochemical, structural insights of newly isolated AA16 family of Lytic Polysaccharide Monooxygenase (LPMO) from <i>Aspergillus fumigatus</i> and investigation of its synergistic effect using biomass
Hossain, Musaddique, Dodda, Subba Reddy, Kapoor, Bishwajit Singh, Aikat, Kaustav, Mukhopadhyay, Sudit S. · 2020 · DOI
Full title: Biochemical, structural insights of newly isolated AA16 family of Lytic Polysaccharide Monooxygenase (LPMO) from <i>Aspergillus fumigatus</i> and investigation of its synergistic effect using biomass
PaperA modular two yeast species secretion system for the production and preparative application of fungal peroxygenases
Püllmann, Pascal, Knorrscheidt, Anja, Münch, Judith, Palme, Paul R., Hoehenwarter, Wolfgang, Marillonnet, Sylvestre, Alcalde, Miguel, Westermann, Bernhard, Weissenborn, Martin J. · 2020 · DOI
Full title: A modular two yeast species secretion system for the production and preparative application of fungal peroxygenases
Authors: Püllmann, Pascal, Knorrscheidt, Anja, Münch, Judith, Palme, Paul R., Hoehenwarter, Wolfgang, Marillonnet, Sylvestre, Alcalde, Miguel, Westermann, Bernhard, Weissenborn, Martin J.
PaperStructural and Functional Comparison of SARS-CoV-2-Spike Receptor Binding Domain Produced in <i>Pichia pastoris</i> and Mammalian Cells
Arbeitman, Claudia R., Auge, Gabriela, Blaustein, Matı́as, Bredeston, Luis M., Corapi, Enrique, Craig, Patricio O., Cossio, Leandro A., Daín, Liliana, D’Alessio, Cecilia, Elias, Fernanda, Fernández, Natalia, Gasulla, Javier, Gorojovsky, Natalia, Gudesblat, Gustavo E., Herrera, María Georgina, Ibañez, Lorena I., Idrovo, Tommy, Randon, Matías Iglesias, Kamenetzky, Laura, Nadra, Alejandro D., Noseda, Diego G., Paván, Carlos H., Pavan, María Florencia, Pignataro, María F., Román, Ernesto A., Ruberto, Lucas, Rubinstein, Natalia, Santos, Javier, Duarte, Francisco Velázquez, Zelada, Alicia M. · 2020 · DOI
Full title: Structural and Functional Comparison of SARS-CoV-2-Spike Receptor Binding Domain Produced in <i>Pichia pastoris</i> and Mammalian Cells
Authors: Arbeitman, Claudia R., Auge, Gabriela, Blaustein, Matı́as, Bredeston, Luis M., Corapi, Enrique, Craig, Patricio O., Cossio, Leandro A., Daín, Liliana, D’Alessio, Cecilia, Elias, Fernanda, Fernández, Natalia, Gasulla, Javier, Gorojovsky, Natalia, Gudesblat, Gustavo E., Herrera, María Georgina, Ibañez, Lorena I., Idrovo, Tommy, Randon, Matías Iglesias, Kamenetzky, Laura, Nadra, Alejandro D., Noseda, Diego G., Paván, Carlos H., Pavan, María Florencia, Pignataro, María F., Román, Ernesto A., Ruberto, Lucas, Rubinstein, Natalia, Santos, Javier, Duarte, Francisco Velázquez, Zelada, Alicia M.
PaperMolecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher · 2020 · DOI
Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Authors: Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher
PaperImproving the heterologous production of fungal peroxygenases through an episomal <i>Pichia pastoris</i> promoter and signal peptide shuffling system
Püllmann, Pascal, Weissenborn, Martin J. · 2020 · DOI
Full title: Improving the heterologous production of fungal peroxygenases through an episomal <i>Pichia pastoris</i> promoter and signal peptide shuffling system
PaperEngineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters
Dalvie, Neil C., Rodriguez‐Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Célia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dong Soo, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanné, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith M., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice, Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher · 2021 · DOI
Full title: Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters
Authors: Dalvie, Neil C., Rodriguez‐Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Célia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dong Soo, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanné, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith M., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice, Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher
PaperScalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered <i>Komagataella phaffii</i>
Dalvie, Neil C., Biedermann, Andrew M., Rodriguez‐Aponte, Sergio A., Naranjo, Christopher A., Rao, Harish, Rajurkar, Meghraj P., Lothe, Rakesh R., Shaligram, Umesh, Johnston, Ryan S., Crowell, Laura E., Castelino, Seraphin, Tracey, Mary Kate, Whittaker, Charles A., Love, J. Christopher · 2021 · Pvt. Ltd. · DOI
Full title: Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered <i>Komagataella phaffii</i>
Authors: Dalvie, Neil C., Biedermann, Andrew M., Rodriguez‐Aponte, Sergio A., Naranjo, Christopher A., Rao, Harish, Rajurkar, Meghraj P., Lothe, Rakesh R., Shaligram, Umesh, Johnston, Ryan S., Crowell, Laura E., Castelino, Seraphin, Tracey, Mary Kate, Whittaker, Charles A., Love, J. Christopher
PaperTime-resolved secretome analysis of three <i>Colletotrichum</i> species identifies copper radical alcohol oxidases for the production of fatty aldehydes
Full title: Time-resolved secretome analysis of three <i>Colletotrichum</i> species identifies copper radical alcohol oxidases for the production of fatty aldehydes
PaperP <sub> <i>AOX1</i> </sub> expression in mixed-substrate continuous cultures of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) is completely determined by methanol consumption regardless of the secondary carbon source
Full title: P <sub> <i>AOX1</i> </sub> expression in mixed-substrate continuous cultures of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) is completely determined by methanol consumption regardless of the secondary carbon source
PaperSteric accessibility of the <i>N</i> -terminus improves the titer and quality of recombinant proteins secreted from <i>Komagataella phaffii</i>
Dalvie, Neil C., Naranjo, Christopher A., Rodriguez‐Aponte, Sergio A., Johnston, Ryan S., Love, J. Christopher · 2022 · DOI
Full title: Steric accessibility of the <i>N</i> -terminus improves the titer and quality of recombinant proteins secreted from <i>Komagataella phaffii</i>
Authors: Dalvie, Neil C., Naranjo, Christopher A., Rodriguez‐Aponte, Sergio A., Johnston, Ryan S., Love, J. Christopher
PaperIdentification, characterisation and recombinant expression of flavonoid 3’,5’-hydroxylases and cytochrome P450 reductases from <i>Vaccinium</i> species
Full title: Identification, characterisation and recombinant expression of flavonoid 3’,5’-hydroxylases and cytochrome P450 reductases from <i>Vaccinium</i> species
PaperHarnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in <i>Komagataella phaffii</i>
Albacar, Marcel, Casamayor, Antonio, Ariño, Joaquı́n · 2023 · DOI
Full title: Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in <i>Komagataella phaffii</i>
PaperThe Mut <sup>+</sup> strain of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) expresses P <i> <sub>AOX1</sub> </i> 5 and 10 times faster than Mut <sup>s</sup> and Mut <sup>−</sup> strains: Evidence that formaldehyde or/and formate are true inducers of AOX
Full title: The Mut <sup>+</sup> strain of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) expresses P <i> <sub>AOX1</sub> </i> 5 and 10 times faster than Mut <sup>s</sup> and Mut <sup>−</sup> strains: Evidence that formaldehyde or/and formate are true inducers of AOX
PaperDisplay of malaria transmission-blocking antigens on chimeric duck hepatitis B virus-derived virus-like particles produced in <i>Hansenula polymorpha</i>
Wetzel, David, Chan, Jo-Anne, Suckow, Manfred, Barbian, Andreas, Weniger, Michael, Jenzelewski, Volker, Reiling, Linda, Richards, Jack S., Anderson, David A., Kouskousis, Betty, Palmer, Catherine S., Hanssen, Eric, Schembecker, Gerhard, Merz, J., Beeson, James G., Piontek, Michael · 2019 · DOI
Full title: Display of malaria transmission-blocking antigens on chimeric duck hepatitis B virus-derived virus-like particles produced in <i>Hansenula polymorpha</i>
Authors: Wetzel, David, Chan, Jo-Anne, Suckow, Manfred, Barbian, Andreas, Weniger, Michael, Jenzelewski, Volker, Reiling, Linda, Richards, Jack S., Anderson, David A., Kouskousis, Betty, Palmer, Catherine S., Hanssen, Eric, Schembecker, Gerhard, Merz, J., Beeson, James G., Piontek, Michael
PaperCharacterization of recombinant human lysosomal beta-hexosaminidases produced in the methylotrophic yeast Pichia pastoris
Espejo, Ángela J., Mosquera, Ángela, Rodríguez‐López, Alexander, Díaz, Dennis, Betrán, Laura Milena, Hernández, Francy Liliana, Alméciga-Díaz, Carlos Javier, Barrera, Luis Alejandro · 2016 · Universitas Scientiarum · DOI
Full title: Characterization of recombinant human lysosomal beta-hexosaminidases produced in the methylotrophic yeast Pichia pastoris
Authors: Espejo, Ángela J., Mosquera, Ángela, Rodríguez‐López, Alexander, Díaz, Dennis, Betrán, Laura Milena, Hernández, Francy Liliana, Alméciga-Díaz, Carlos Javier, Barrera, Luis Alejandro
Full title: Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso
PaperAdaptation of Hansenula polymorpha to methanol: a transcriptome analysis
Zutphen, Tim van, Baerends, Richard J. S., Susanna, Kim A., Jong, Anne de, Kuipers, Oscar P., Veenhuis, Marten, Klei, Ida J. van der · 2010 · BMC Genomics · DOI
Full title: Adaptation of Hansenula polymorpha to methanol: a transcriptome analysis
Authors: Zutphen, Tim van, Baerends, Richard J. S., Susanna, Kim A., Jong, Anne de, Kuipers, Oscar P., Veenhuis, Marten, Klei, Ida J. van der
PaperGenome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1
Ravin, Nikolai V., Eldarov, Michael A., Kadnikov, Vitaly V., Beletsky, Alexey V., Schneider, Jessica, Mardanova, Eugenia S., Smekalova, Elena M., Zvereva, Maria I., Донцова, О. А., Mardanov, Andrey V., Skryabin, K. G. · 2013 · BMC Genomics · DOI
Full title: Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1
Authors: Ravin, Nikolai V., Eldarov, Michael A., Kadnikov, Vitaly V., Beletsky, Alexey V., Schneider, Jessica, Mardanova, Eugenia S., Smekalova, Elena M., Zvereva, Maria I., Донцова, О. А., Mardanov, Andrey V., Skryabin, K. G.
PaperThe alkaline pectate lyase PEL168 of Bacillus subtilis heterologously expressed in Pichia pastorisis more stable and efficient for degumming ramie fiber
Full title: The alkaline pectate lyase PEL168 of Bacillus subtilis heterologously expressed in Pichia pastorisis more stable and efficient for degumming ramie fiber
PaperExpression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris
Canales, Mario, Lastra, José Manuel Pérez de la, Naranjo, Victoria, Nijhof, Ard M., Hope, Michelle, Jongejan, Frans, Fuente, José de la · 2008 · BMC Biotechnology · DOI
Full title: Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris
Authors: Canales, Mario, Lastra, José Manuel Pérez de la, Naranjo, Victoria, Nijhof, Ard M., Hope, Michelle, Jongejan, Frans, Fuente, José de la
PaperHeterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes
Bey, Mathieu, Berrin, Jean‐Guy, Poidevin, Laetitia, Sigoillot, Jean‐Claude · 2011 · Microbial Cell Factories · DOI
Full title: Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes
Authors: Bey, Mathieu, Berrin, Jean‐Guy, Poidevin, Laetitia, Sigoillot, Jean‐Claude
Full title: Development of a cultivation process for the enhancement of human interferon alpha 2b production in the oleaginous yeast, Yarrowia lipolytica
PaperPhysiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes
Full title: Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes
Full title: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES
PaperA screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter
Nallaseth, Ferez S., Anderson, Stephen K. · 2013 · Microbial Cell Factories · DOI
Full title: A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter
Authors: Nallaseth, Ferez S., Anderson, Stephen K.
PaperTowards better understanding of an industrial cell factory: investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris
Fazenda, Mariana L., Dias, João M.L., Harvey, Linda M., Nordon, Alison, Edrada‐Ebel, RuAngelie, Littlejohn, David, McNeil, Brian · 2013 · Microbial Cell Factories · DOI
Full title: Towards better understanding of an industrial cell factory: investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris
Authors: Fazenda, Mariana L., Dias, João M.L., Harvey, Linda M., Nordon, Alison, Edrada‐Ebel, RuAngelie, Littlejohn, David, McNeil, Brian
PaperExometabolome analysis reveals hypoxia at the up-scaling of a Saccharomyces cerevisiae high-cell density fed-batch biopharmaceutical process
Fu, Zhibiao, Verderame, Thomas D, Leighton, Julie, Sampey, Brante P., Appelbaum, Edward R., Patel, Pramatesh S., Aon, Juan C. · 2014 · Microbial Cell Factories · DOI
Full title: Exometabolome analysis reveals hypoxia at the up-scaling of a Saccharomyces cerevisiae high-cell density fed-batch biopharmaceutical process
Authors: Fu, Zhibiao, Verderame, Thomas D, Leighton, Julie, Sampey, Brante P., Appelbaum, Edward R., Patel, Pramatesh S., Aon, Juan C.
PaperComprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase
Hemmerich, Johannes, Adelantado, Núria, Barrigón, José Manuel, Ponte, Xavier, Hörmann, Astrid, Ferrer, Pau, Kensy, Frank, Valero, Francisco · 2014 · Microbial Cell Factories · DOI
Full title: Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase
Authors: Hemmerich, Johannes, Adelantado, Núria, Barrigón, José Manuel, Ponte, Xavier, Hörmann, Astrid, Ferrer, Pau, Kensy, Frank, Valero, Francisco
PaperRole of cultivation media in the development of yeast strains for large scale industrial use
Hahn‐Hägerdal, Bärbel, Karhumaa, Kaisa, Larsson, Christer, Gorwa‐Grauslund, Marie F., Görgens, Johann F., Zyl, Willem H. van · 2005 · Microbial Cell Factories · DOI
Full title: Role of cultivation media in the development of yeast strains for large scale industrial use
Authors: Hahn‐Hägerdal, Bärbel, Karhumaa, Kaisa, Larsson, Christer, Gorwa‐Grauslund, Marie F., Görgens, Johann F., Zyl, Willem H. van
PaperOperational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review
Cós, Oriol, Ramón, Ramón, Montesinos, José Luis González, Valero, Francisco · 2006 · Microbial Cell Factories · DOI
Full title: Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review
Authors: Cós, Oriol, Ramón, Ramón, Montesinos, José Luis González, Valero, Francisco
PaperTranscriptional analysis of protein production and induction of unfolded protein response in Pichia pastoris expressing a Rhizopus oryzae lipase under the FLD1 promoter
David Resina, M. Bollók, F. Valero, P. Neubauer, P. Ferrer · 2006 · Microbial Cell Factories · DOI
Full title: Transcriptional analysis of protein production and induction of unfolded protein response in Pichia pastoris expressing a Rhizopus oryzae lipase under the FLD1 promoter
Authors: David Resina, M. Bollók, F. Valero, P. Neubauer, P. Ferrer
PaperAnalysis of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated formaldehyde dehydrogenase promoter (PFLD1)
Full title: Analysis of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated formaldehyde dehydrogenase promoter (PFLD1)
PaperSubstrate feeding strategies in Pichia pastoris fed-batch cultivation processes: Analysis of key parameters influencing recombinant protein production
Ramón, Ramón, Cós, Oriol, Ferrer, Pau, Montesinos, José Luis González, Valero, Francisco · 2006 · Microbial Cell Factories · DOI
Full title: Substrate feeding strategies in Pichia pastoris fed-batch cultivation processes: Analysis of key parameters influencing recombinant protein production
Authors: Ramón, Ramón, Cós, Oriol, Ferrer, Pau, Montesinos, José Luis González, Valero, Francisco
PaperSimple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen
Full title: Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen
PaperDeveloping a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime
Holmes, William J., Darby, Richard A. J., Wilks, Martin DB, Smith, Rodney, Bill, Roslyn M. · 2009 · Microbial Cell Factories · DOI
Full title: Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime
Authors: Holmes, William J., Darby, Richard A. J., Wilks, Martin DB, Smith, Rodney, Bill, Roslyn M.
Full title: Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-β-mannosidase from Aspergillus niger BK01
Full title: Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels
PaperPerformance of the auxotrophic Saccharomyces cerevisiae BY4741 as host for the production of IL-1β in aerated fed-batch reactor: role of ACA supplementation, strain viability, and maintenance energy
Full title: Performance of the auxotrophic Saccharomyces cerevisiae BY4741 as host for the production of IL-1β in aerated fed-batch reactor: role of ACA supplementation, strain viability, and maintenance energy
PaperApplication of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin
Chandrasekhar Gurramkonda, Sulena Polez, N. Skoko, Ahmad Adnan, Thomas Gäbel, Dipti A. Chugh, S. Swaminathan, N. Khanna, S. Tisminetzky, U. Rinas · 2010 · Microbial Cell Factories · DOI
Full title: Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin
Authors: Chandrasekhar Gurramkonda, Sulena Polez, N. Skoko, Ahmad Adnan, Thomas Gäbel, Dipti A. Chugh, S. Swaminathan, N. Khanna, S. Tisminetzky, U. Rinas
PaperExpression of an extremely acidic beta-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence.
Full title: Expression of an extremely acidic beta-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence.
PaperHigh yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture.
Chen, Ruidong, Zhou, Zhigang, Cao, Yanan, Bai, Yingguo, Yao, Bin · 2010 · Microbial cell factories · DOI
Full title: High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture.
PaperFed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate
Pieter P. Jacobs, Mehmet Inan, N. Festjens, J. Haustraete, Annelies Van Hecke, R. Contreras, M. Meagher, N. Callewaert · 2010 · Microbial Cell Factories · DOI
Full title: Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate
Authors: Pieter P. Jacobs, Mehmet Inan, N. Festjens, J. Haustraete, Annelies Van Hecke, R. Contreras, M. Meagher, N. Callewaert
PaperGlucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary 13C flux analysis
Jordà, Joel, Suarez-Mendez, Camilo, Carnicer, Marc, Pierick, Angela ten, Heijnen, Joseph J., Gulik, Walter van, Ferrer, Pau, Albiol, Joan, Wahl, A. · 2013 · BMC Systems Biology · DOI
Full title: Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary 13C flux analysis
Authors: Jordà, Joel, Suarez-Mendez, Camilo, Carnicer, Marc, Pierick, Angela ten, Heijnen, Joseph J., Gulik, Walter van, Ferrer, Pau, Albiol, Joan, Wahl, A.
PaperOptimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33
Full title: Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33
PaperA new acidophilic thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris
Full title: A new acidophilic thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris
PaperHigh-level expression, purification, and enzymatic characterization of truncated human plasminogen (Lys531-Asn791) in the methylotrophic yeast Pichia pastoris
Full title: High-level expression, purification, and enzymatic characterization of truncated human plasminogen (Lys531-Asn791) in the methylotrophic yeast Pichia pastoris
PaperProduction of human pro-relaxin H2 in the yeast Pichia pastoris
D. Cimini, K. Corte, R. Finamore, L. Andreozzi, A. Stellavato, A. Pirozzi, F. Ferrara, R. Formisano, M. de Rosa, M. Chino, L. Lista, A. Lombardi, V. Pavone, C. Schiraldi · 2017 · BMC Biotechnology · DOI
Full title: Production of human pro-relaxin H2 in the yeast Pichia pastoris
Authors: D. Cimini, K. Corte, R. Finamore, L. Andreozzi, A. Stellavato, A. Pirozzi, F. Ferrara, R. Formisano, M. de Rosa, M. Chino, L. Lista, A. Lombardi, V. Pavone, C. Schiraldi
PaperHeterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring
Full title: Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring
Full title: Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation
PaperHeterologous expression and biochemical characterization of a GHF9 endoglucanase from the termite Reticulitermes speratus in Pichia pastoris
Zhang, Pengfei, Yuan, Xianghua, Du, Yuguang, Li, Jianjun · 2018 · BMC Biotechnology · DOI
Full title: Heterologous expression and biochemical characterization of a GHF9 endoglucanase from the termite Reticulitermes speratus in Pichia pastoris
Authors: Zhang, Pengfei, Yuan, Xianghua, Du, Yuguang, Li, Jianjun
PaperAn effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)
Che, Zhiqun, Cao, Xiaoyan, Chen, Guiguang, Liang, Zhiqun · 2020 · BMC Biotechnology · DOI
Full title: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)
Authors: Che, Zhiqun, Cao, Xiaoyan, Chen, Guiguang, Liang, Zhiqun
PaperFunctional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks
Bawa, Zharain, Routledge, Sarah J, Jamshad, Mohammed, Clare, Michelle, Sarkar, Debasmita, Dickerson, Ian M., Ganzlin, Markus, Poyner, David R., Bill, Roslyn M. · 2014 · Microbial Cell Factories · DOI
Full title: Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks
Authors: Bawa, Zharain, Routledge, Sarah J, Jamshad, Mohammed, Clare, Michelle, Sarkar, Debasmita, Dickerson, Ian M., Ganzlin, Markus, Poyner, David R., Bill, Roslyn M.
PaperHigh-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities
Full title: High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities
PaperHigh-temperature cultivation of recombinant Pichia pastoris increases endoplasmic reticulum stress and decreases production of human interleukin-10.
Full title: High-temperature cultivation of recombinant Pichia pastoris increases endoplasmic reticulum stress and decreases production of human interleukin-10.
PaperAn artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures
Pedro, Augusto Q., Martins, Luís C., Dias, João M.L., Bonifácio, Maria João, Queiroz, João A., Passarinha, Luís A. · 2015 · Microbial Cell Factories · DOI
Full title: An artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures
Authors: Pedro, Augusto Q., Martins, Luís C., Dias, João M.L., Bonifácio, Maria João, Queiroz, João A., Passarinha, Luís A.
PaperIntegrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger
Full title: Integrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger
PaperPichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source
Schotte, Peter, Dewerte, Isabelle, Groeve, Manu De, Keyser, Saskia De, Brabandere, Veronique De, Stanssens, Patrick · 2016 · Microbial Cell Factories · DOI
Full title: Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source
Authors: Schotte, Peter, Dewerte, Isabelle, Groeve, Manu De, Keyser, Saskia De, Brabandere, Veronique De, Stanssens, Patrick
PaperOptimization of construct design and fermentation strategy for the production of bioactive ATF-SAP, a saporin based anti-tumoral uPAR-targeted chimera
Full title: Optimization of construct design and fermentation strategy for the production of bioactive ATF-SAP, a saporin based anti-tumoral uPAR-targeted chimera
PaperMolecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems
Ma, Su, Preims, Marita, Piumi, François, Kappel, Lisa, Seiboth, Bernhard, Record, Éric, Kracher, Daniel, Ludwig, Roland · 2017 · Microbial Cell Factories · DOI
Full title: Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems
Authors: Ma, Su, Preims, Marita, Piumi, François, Kappel, Lisa, Seiboth, Bernhard, Record, Éric, Kracher, Daniel, Ludwig, Roland
PaperImplications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris
Moser, Josef W., Prielhofer, Roland, Gerner, Samuel M., Graf, Alexandra B., Wilson, Iain B. H., Mattanovich, Diethard, Dragosits, Martin · 2017 · Microbial Cell Factories · DOI
Full title: Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris
Authors: Moser, Josef W., Prielhofer, Roland, Gerner, Samuel M., Graf, Alexandra B., Wilson, Iain B. H., Mattanovich, Diethard, Dragosits, Martin
Full title: Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison.
PaperRecombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection.
Bando-Campos, Giroshi, Juárez-López, Daniel, Román-González, Sergio A, Castillo-Rodal, Antonia I, Olvera, Clarita, López-Vidal, Yolanda, Arreguín-Espinosa, Roberto, Espitia, Clara, Trujillo-Roldán, Mauricio A, Valdez-Cruz, Norma A · 2019 · Microbial cell factories · DOI
Full title: Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection.
Authors: Bando-Campos, Giroshi, Juárez-López, Daniel, Román-González, Sergio A, Castillo-Rodal, Antonia I, Olvera, Clarita, López-Vidal, Yolanda, Arreguín-Espinosa, Roberto, Espitia, Clara, Trujillo-Roldán, Mauricio A, Valdez-Cruz, Norma A
Full title: Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris
PaperCharacterization of a highly stable α-galactosidase from thermophilic Rasamsonia emersonii heterologously expressed in a modified Pichia pastoris expression system
An, Jian-Lu, Zhang, Weixin, Wu, Weiping, Chen, Guanjun, Liu, Weifeng · 2019 · Microbial Cell Factories · DOI
Full title: Characterization of a highly stable α-galactosidase from thermophilic Rasamsonia emersonii heterologously expressed in a modified Pichia pastoris expression system
PaperSpecific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage
Garrigós‐Martínez, Javier, Nieto‐Taype, Miguel Angel, Gasset-Franch, Arnau, Montesinos, José Luis González, Garcia‐Ortega, Xavier, Valero, Francisco · 2019 · Microbial Cell Factories · DOI
Full title: Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage
Authors: Garrigós‐Martínez, Javier, Nieto‐Taype, Miguel Angel, Gasset-Franch, Arnau, Montesinos, José Luis González, Garcia‐Ortega, Xavier, Valero, Francisco
PaperCo-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone
Full title: Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone
Full title: Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc
PaperDifferential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene
Full title: Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene
PaperProduction of bio-xylitol from d-xylose by an engineered Pichia pastoris expressing a recombinant xylose reductase did not require any auxiliary substrate as electron donor
Louie, Tai Man, Louie, Kailin, DenHartog, Samuel, Gopishetty, Sridhar, Subramanian, Mani, Arnold, Mark A., Das, Shuvendu · 2021 · Microbial Cell Factories · DOI
Full title: Production of bio-xylitol from d-xylose by an engineered Pichia pastoris expressing a recombinant xylose reductase did not require any auxiliary substrate as electron donor
Authors: Louie, Tai Man, Louie, Kailin, DenHartog, Samuel, Gopishetty, Sridhar, Subramanian, Mani, Arnold, Mark A., Das, Shuvendu
PaperMolecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher · 2021 · Microbial Cell Factories · DOI
Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Authors: Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher
PaperSimultaneous enzyme production, Levan-type FOS synthesis and sugar by-products elimination using a recombinant Pichia pastoris strain expressing a levansucrase-endolevanase fusion enzyme
Ávila-Fernández, Ángela, Montiel, Silvia, Rodríguez-Alegría, Maria Elena, Caspeta, Luis, López-Munguı́a, Agustı́n · 2023 · Microbial Cell Factories · DOI
Full title: Simultaneous enzyme production, Levan-type FOS synthesis and sugar by-products elimination using a recombinant Pichia pastoris strain expressing a levansucrase-endolevanase fusion enzyme
PaperBiosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris
Navone, Laura, Moffitt, Kaylee, Behrendorff, James B. Y. H., Sadowski, Paweł, Hartley, Carol J., Speight, Robert · 2023 · Microbial Cell Factories · DOI
Full title: Biosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris
Authors: Navone, Laura, Moffitt, Kaylee, Behrendorff, James B. Y. H., Sadowski, Paweł, Hartley, Carol J., Speight, Robert
PaperHigh throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux
Full title: High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux
Paper‘Mother(Nature) knows best’ – hijacking nature-designed transcriptional programs for enhancing stress resistance and protein production in Yarrowia lipolytica; presentation of YaliFunTome database
Gorczyca, Maria, Białas, W., Nicaud, Jean‐Marc, Celińska, Ewelina · 2024 · Microbial Cell Factories · DOI
Full title: ‘Mother(Nature) knows best’ – hijacking nature-designed transcriptional programs for enhancing stress resistance and protein production in Yarrowia lipolytica; presentation of YaliFunTome database
Authors: Gorczyca, Maria, Białas, W., Nicaud, Jean‐Marc, Celińska, Ewelina
PaperImpact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications
Full title: Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications
Full title: Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii
PaperCRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Dalvie, Neil C., Lorgeree, Timothy, Yang, Yuchen, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Hinckley, Joshua A., Clark, John J, Rosario, Amanda Del, Love, Kerry R., Love, J. Christopher · 2024 · Microbial Cell Factories · DOI
Full title: CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Authors: Dalvie, Neil C., Lorgeree, Timothy, Yang, Yuchen, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Hinckley, Joshua A., Clark, John J, Rosario, Amanda Del, Love, Kerry R., Love, J. Christopher
PaperDevelopment of an efficient expression system for human chaperone BiP in Pichia pastoris: production optimization and functional validation
Eimantas Žitkus, E. Ciplys, Mantas Žiaunys, A. Sakalauskas, R. Slibinskas · 2025 · Microbial Cell Factories · DOI
Full title: Development of an efficient expression system for human chaperone BiP in Pichia pastoris: production optimization and functional validation
Authors: Eimantas Žitkus, E. Ciplys, Mantas Žiaunys, A. Sakalauskas, R. Slibinskas
PaperDisulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris
Navone, Laura, Vogl, Thomas, Luangthongkam, Pawarisa, Blinco, Jo-Anne, Luna‐Flores, Carlos H., Chen, Xiaojing, Hellens, Juhani von, Mahler, Stephen M., Speight, Robert · 2021 · Biotechnology for Biofuels · DOI
Full title: Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris
Authors: Navone, Laura, Vogl, Thomas, Luangthongkam, Pawarisa, Blinco, Jo-Anne, Luna‐Flores, Carlos H., Chen, Xiaojing, Hellens, Juhani von, Mahler, Stephen M., Speight, Robert
PaperImprovements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution
Kao, Mu‐Rong, Yu, Su‐May, Ho, Tuan-H ua David · 2021 · Biotechnology for Biofuels · DOI
Full title: Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution
Authors: Kao, Mu‐Rong, Yu, Su‐May, Ho, Tuan-H ua David
PaperPichia pastoris versus Saccharomyces cerevisiae: a case study on the recombinant production of human granulocyte-macrophage colony-stimulating factor.
Tran, Anh-Minh, Nguyen, Thanh-Thao, Nguyen, Cong-Thuan, Huynh-Thi, Xuan-Mai, Nguyen, Cao-Tri, Trinh, Minh-Thuong, Tran, Linh-Thuoc, Cartwright, Stephanie P, Bill, Roslyn M, Tran-Van, Hieu · 2017 · BMC research notes · DOI
Full title: Pichia pastoris versus Saccharomyces cerevisiae: a case study on the recombinant production of human granulocyte-macrophage colony-stimulating factor.
Full title: Expression of a highly active β-glucosidase from Aspergillus niger AS3.4523 in Escherichia coli and its application in gardenia blue preparation
PaperCoexpression of cellulases in Pichia pastoris as a self-processing protein fusion
Araújo, Juliana de Amorim, Ferreira, Túlio César, Rubini, Marciano Régis, Duran, Ana Gilhema Gomez, Marco, Janice Lisboa De, Moraes, Lídia Maria Pepe de, Torres, Fernando Araripe Gonçalves · 2015 · AMB Express · DOI
Full title: Coexpression of cellulases in Pichia pastoris as a self-processing protein fusion
Authors: Araújo, Juliana de Amorim, Ferreira, Túlio César, Rubini, Marciano Régis, Duran, Ana Gilhema Gomez, Marco, Janice Lisboa De, Moraes, Lídia Maria Pepe de, Torres, Fernando Araripe Gonçalves
PaperExpression of a new laccase from Moniliophthora roreri at high levels in Pichia pastoris and its potential application in micropollutant degradation
Bronikowski, Agathe, Hagedoorn, Peter‐Leon, Koschorreck, Katja, Urlacher, Vlada B. · 2017 · AMB Express · DOI
Full title: Expression of a new laccase from Moniliophthora roreri at high levels in Pichia pastoris and its potential application in micropollutant degradation
Authors: Bronikowski, Agathe, Hagedoorn, Peter‐Leon, Koschorreck, Katja, Urlacher, Vlada B.
PaperIdentification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability.
Kangwa, Martin, Salgado, Jose Antonio Gama, Fernandez-Lahore, Hector Marcelo · 2018 · AMB Express · DOI
Full title: Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability.
Authors: Kangwa, Martin, Salgado, Jose Antonio Gama, Fernandez-Lahore, Hector Marcelo
PaperCombined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris
Full title: Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris
PaperEnhancing the secretion pathway maximizes the effects of mixed feeding strategy for glucose oxidase production in the methylotrophic yeast Pichia pastoris
Bankefa, Olufemi Emmanuel, Wang, Meiyu, Zhu, Taicheng, Li, Yin · 2018 · Bioresources and Bioprocessing · DOI
Full title: Enhancing the secretion pathway maximizes the effects of mixed feeding strategy for glucose oxidase production in the methylotrophic yeast Pichia pastoris
PaperImproved microscale cultivation of Pichia pastoris for clonal screening
Eck, Alexander, Schmidt, Matthias, Hamer, Stefanie Nicole, Ruff, Anna Joëlle, Förster, Jan Janosch, Schwaneberg, Ulrich, Blank, Lars M., Wiechert, Wolfgang, Oldiges, Marco · 2018 · Fungal Biology and Biotechnology · DOI
Full title: Improved microscale cultivation of Pichia pastoris for clonal screening
Authors: Eck, Alexander, Schmidt, Matthias, Hamer, Stefanie Nicole, Ruff, Anna Joëlle, Förster, Jan Janosch, Schwaneberg, Ulrich, Blank, Lars M., Wiechert, Wolfgang, Oldiges, Marco
PaperAn improved expression and purification protocol enables the structural characterization of Mnt1, an antifungal target from Candida albicans.
Silva, Patrícia Alves, Souza, Amanda Araújo, de Oliveira, Gideane Mendes, Ramada, Marcelo Henrique Soller, Hernández, Nahúm Valente, Mora-Montes, Héctor Manuel, Bueno, Renata Vieira, Martins-de-Sa, Diogo, de Freitas, Sonia Maria, Felipe, Maria Sueli Soares, Barbosa, João Alexandre Ribeiro Gonçalves · 2024 · Fungal biology and biotechnology · DOI
Full title: An improved expression and purification protocol enables the structural characterization of Mnt1, an antifungal target from Candida albicans.
Authors: Silva, Patrícia Alves, Souza, Amanda Araújo, de Oliveira, Gideane Mendes, Ramada, Marcelo Henrique Soller, Hernández, Nahúm Valente, Mora-Montes, Héctor Manuel, Bueno, Renata Vieira, Martins-de-Sa, Diogo, de Freitas, Sonia Maria, Felipe, Maria Sueli Soares, Barbosa, João Alexandre Ribeiro Gonçalves
PaperRational Design of a Fibroblast Growth Factor 21-Based Clinical Candidate, LY2405319
Kharitonenkov, Alexei, Beals, John M., Micanovic, Radmila, Strifler, Beth A., Rathnachalam, Radhakrishnan, Wroblewski, Victor J., Li, Shun, Köester, Anja, Ford, Amy, Coşkun, Tamer, Dunbar, James D., Cheng, Christine C., Frye, Christopher C., Bumol, Thomas F., Moller, David E. · 2013 · PLoS ONE · DOI
Full title: Rational Design of a Fibroblast Growth Factor 21-Based Clinical Candidate, LY2405319
Authors: Kharitonenkov, Alexei, Beals, John M., Micanovic, Radmila, Strifler, Beth A., Rathnachalam, Radhakrishnan, Wroblewski, Victor J., Li, Shun, Köester, Anja, Ford, Amy, Coşkun, Tamer, Dunbar, James D., Cheng, Christine C., Frye, Christopher C., Bumol, Thomas F., Moller, David E.
PaperDetergent Screening and Purification of the Human Liver ABC Transporters BSEP (ABCB11) and MDR3 (ABCB4) Expressed in the Yeast Pichia pastoris
Ellinger, Philipp, Kluth, Marianne, Stindt, Jan, Smits, Sander H. J., Schmitt, Lutz · 2013 · PLoS ONE · DOI
Full title: Detergent Screening and Purification of the Human Liver ABC Transporters BSEP (ABCB11) and MDR3 (ABCB4) Expressed in the Yeast Pichia pastoris
PaperCharacterization of the Pichia pastoris Protein-O-mannosyltransferase Gene Family
Nett, Juergen H., Cook, W. James, Mingtang, Chen, Davidson, Robert C., Bobrowicz, Piotr, Kett, Warren C., Brevnova, Elena E., Potgieter, Thomas I., Mellon, Mark T., Prinz, Bianka, Choi, Byung‐Kwon, Zha, Dongxing, Burnina, Irina, Bukowski, John, Du, Min, Wildt, Stefan, Hamilton, Stephen R. · 2013 · PLoS ONE · DOI
Full title: Characterization of the Pichia pastoris Protein-O-mannosyltransferase Gene Family
Authors: Nett, Juergen H., Cook, W. James, Mingtang, Chen, Davidson, Robert C., Bobrowicz, Piotr, Kett, Warren C., Brevnova, Elena E., Potgieter, Thomas I., Mellon, Mark T., Prinz, Bianka, Choi, Byung‐Kwon, Zha, Dongxing, Burnina, Irina, Bukowski, John, Du, Min, Wildt, Stefan, Hamilton, Stephen R.
PaperGlycosylation variants of a β-glucosidase secreted by a Taiwanese fungus, Chaetomella raphigera, exhibit variant-specific catalytic and biochemical properties.
Yoneda, Aki, Kuo, Hsion-Wen David, Ishihara, Mayumi, Azadi, Parastoo, Yu, Su-May, Ho, Tuan-hua David · 2014 · PloS one · DOI
Full title: Glycosylation variants of a β-glucosidase secreted by a Taiwanese fungus, Chaetomella raphigera, exhibit variant-specific catalytic and biochemical properties.
PaperThe pCri System: A Vector Collection for Recombinant Protein Expression and Purification
Goulas, Theodoros, Cuppari, Anna, García-Castellanos, Raquel, Snipas, Scott J., Glockshuber, Rudi, Arolas, Joan L., Gomis‐Rüth, F. Xavier · 2014 · PLoS ONE · DOI
Full title: The pCri System: A Vector Collection for Recombinant Protein Expression and Purification
Authors: Goulas, Theodoros, Cuppari, Anna, García-Castellanos, Raquel, Snipas, Scott J., Glockshuber, Rudi, Arolas, Joan L., Gomis‐Rüth, F. Xavier
PaperPurification, cloning, characterization, and N-glycosylation analysis of a novel β-fructosidase from Aspergillus oryzae FS4 synthesizing levan- and neolevan-type fructooligosaccharides.
Xu, Li, Wang, Dongxue, Lu, Lili, Jin, Lan, Liu, Jiawei, Song, Deyong, Guo, Zhongwu, Xiao, Min · 2014 · PloS one · DOI
Full title: Purification, cloning, characterization, and N-glycosylation analysis of a novel β-fructosidase from Aspergillus oryzae FS4 synthesizing levan- and neolevan-type fructooligosaccharides.
PaperComputational Analysis and Low-Scale Constitutive Expression of Laccases Synthetic Genes GlLCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris
Rivera-Hoyos, Claudia M., Morales-Álvarez, Edwin D., Cuevas, Sergio Alejandro Poveda, Reyes-Guzmán, Edwin Alfredo, Poutou‐Piñales, Raúl A., Reyes-Montaño, Edgar Antonio, Pedroza-Rodríguez, Aura M., Rodríguez-Vázquez, Refugio, Cardozo-Bernal, Ángela M. · 2015 · PLoS ONE · DOI
Full title: Computational Analysis and Low-Scale Constitutive Expression of Laccases Synthetic Genes GlLCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris
Authors: Rivera-Hoyos, Claudia M., Morales-Álvarez, Edwin D., Cuevas, Sergio Alejandro Poveda, Reyes-Guzmán, Edwin Alfredo, Poutou‐Piñales, Raúl A., Reyes-Montaño, Edgar Antonio, Pedroza-Rodríguez, Aura M., Rodríguez-Vázquez, Refugio, Cardozo-Bernal, Ángela M.
PaperTranslational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
Edwards-Jones, Bryn, Aw, Rochelle, Barton, Geraint, Tredwell, Gregory D., Bundy, Jacob G., Leak, David J. · 2015 · PLoS ONE · DOI
Full title: Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
Authors: Edwards-Jones, Bryn, Aw, Rochelle, Barton, Geraint, Tredwell, Gregory D., Bundy, Jacob G., Leak, David J.
PaperOverexpression of a Novel Thermostable and Chloride-Tolerant Laccase from Thermus thermophilus SG0.5JP17-16 in Pichia pastoris and Its Application in Synthetic Dye Decolorization
Liu, Huiping, Cheng, Yu, Du, Bing, Tong, Chaofan, Liang, Shuli, Han, Shuangyan, Zheng, Suiping, Lin, Ying · 2015 · PLoS ONE · DOI
Full title: Overexpression of a Novel Thermostable and Chloride-Tolerant Laccase from Thermus thermophilus SG0.5JP17-16 in Pichia pastoris and Its Application in Synthetic Dye Decolorization
PaperHigh-Level Expression of Endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Application for the Deglycosylation of Glycoproteins
Fei Wang, Xiaojuan Wang, Xiaolan Yu, Ling Fu, Yunyun Liu, Lixin Ma, C. Zhai · 2015 · PLoS ONE · DOI
Full title: High-Level Expression of Endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Application for the Deglycosylation of Glycoproteins
PaperCharacterization of a Recombinant Cathepsin B-Like Cysteine Peptidase from Diaphorina citri Kuwayama (Hemiptera: Liviidae): A Putative Target for Control of Citrus Huanglongbing
Ferrara, Taíse Fernanda da Silva, Schneider, Vanessa Karine, Kishi, Luciano Takeshi, Carmona, Adriana K., Alves, Márcio F.M., Belasque, José, Rosa, José Cesar, Hunter, Wayne B., Henrique‐Silva, Flávio, Soares‐Costa, Andrea · 2015 · PLoS ONE · DOI
Full title: Characterization of a Recombinant Cathepsin B-Like Cysteine Peptidase from Diaphorina citri Kuwayama (Hemiptera: Liviidae): A Putative Target for Control of Citrus Huanglongbing
Authors: Ferrara, Taíse Fernanda da Silva, Schneider, Vanessa Karine, Kishi, Luciano Takeshi, Carmona, Adriana K., Alves, Márcio F.M., Belasque, José, Rosa, José Cesar, Hunter, Wayne B., Henrique‐Silva, Flávio, Soares‐Costa, Andrea
PaperCloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E. coli.
Pal Roy, Moushree, Mazumdar, Deepika, Dutta, Subhabrata, Saha, Shyama Prasad, Ghosh, Shilpi · 2016 · PloS one · DOI
Full title: Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E. coli.
PaperIntegration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism
Tomàs‐Gamisans, Màrius, Ferrer, Pau, Albiol, Joan · 2016 · PLoS ONE · DOI
Full title: Integration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism
Authors: Tomàs‐Gamisans, Màrius, Ferrer, Pau, Albiol, Joan
PaperRole of N-linked glycosylation in the enzymatic properties of a thermophilic GH 10 xylanase from Aspergillus fumigatus expressed in Pichia pastoris.
Chang, Xiaoyu, Xu, Bo, Bai, Yingguo, Luo, Huiying, Ma, Rui, Shi, Pengjun, Yao, Bin · 2017 · PloS one · DOI
Full title: Role of N-linked glycosylation in the enzymatic properties of a thermophilic GH 10 xylanase from Aspergillus fumigatus expressed in Pichia pastoris.
PaperA quantitative indicator diagram for lytic polysaccharide monooxygenases reveals the role of aromatic surface residues in HjLPMO9A regioselectivity
Danneels, Barbara, Tanghe, Magali, Joosten, Henk‐Jan, Gundinger, Thomas, Spadiut, Oliver, Stals, Ingeborg, Desmet, Tom · 2017 · PLoS ONE · DOI
Full title: A quantitative indicator diagram for lytic polysaccharide monooxygenases reveals the role of aromatic surface residues in HjLPMO9A regioselectivity
PaperEnhancing monellin production by Pichia pastoris at low cell induction concentration via effectively regulating methanol metabolism patterns and energy utilization efficiency
Full title: Enhancing monellin production by Pichia pastoris at low cell induction concentration via effectively regulating methanol metabolism patterns and energy utilization efficiency
PaperModulation of heterologous protein secretion in the thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC 656 by CRISPR-Cas9 system
Kruasuwan, Worarat, Puseenam, Aekkachai, Phithakrotchanakoon, Chitwadee, Tanapongpipat, Sutipa, Roongsawang, Niran · 2021 · PLoS ONE · DOI
Full title: Modulation of heterologous protein secretion in the thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC 656 by CRISPR-Cas9 system
PaperHigh expression of human carboxypeptidase M in Pichia pastoris: purification and partial characterization.
Craveiro, R B, Ramalho, J D, Chagas, J R, Wang, P H M, Casarini, D, Pesquero, J L, Araújo, R C, Pesquero, J B · 2006 · Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · DOI
Full title: High expression of human carboxypeptidase M in Pichia pastoris: purification and partial characterization.
Authors: Craveiro, R B, Ramalho, J D, Chagas, J R, Wang, P H M, Casarini, D, Pesquero, J L, Araújo, R C, Pesquero, J B
Year: 2006
Venue: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica
PaperExpression of Pisum sativum defensin 1 (Psd1) in shaking flasks and bioreactor cultivations of recombinant Pichia pastoris at different pHs
Larentis, Ariane Leites, Almeida, Marcius S., Cabral, Kátia M.S., Medeiros, Luciano Neves de, Kurtenbach, Eleonora, Coelho, Maria Alice Zarur · 2004 · Brazilian Journal of Chemical Engineering · DOI
Full title: Expression of Pisum sativum defensin 1 (Psd1) in shaking flasks and bioreactor cultivations of recombinant Pichia pastoris at different pHs
Authors: Larentis, Ariane Leites, Almeida, Marcius S., Cabral, Kátia M.S., Medeiros, Luciano Neves de, Kurtenbach, Eleonora, Coelho, Maria Alice Zarur
PaperConstitutive High Expression Level of A Synthetic Deleted Encoding Gene of <i>Talaromyces minioluteus</i> Endodextranase Variant (r-TmDEX49A-ΔSP-ΔN30) in <i>Komagataella phaffii</i> (<i>Pichia pastoris</i>)
Full title: Constitutive High Expression Level of A Synthetic Deleted Encoding Gene of <i>Talaromyces minioluteus</i> Endodextranase Variant (r-TmDEX49A-ΔSP-ΔN30) in <i>Komagataella phaffii</i> (<i>Pichia pastoris</i>)
PaperDevelopment of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain (RBD) Using Pichia pastoris in Stirred-Tank Bioreactor
Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, María Florencia, Wetzler, Diana E., Gentilli, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas AM · 2023 · Preprints.org · DOI
Full title: Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain (RBD) Using Pichia pastoris in Stirred-Tank Bioreactor
Authors: Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, María Florencia, Wetzler, Diana E., Gentilli, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas AM
Full title: Production of Plant-Based Meat Flavor Compound – Leghemo-Globin (LegH) in Recombinant <em>Komagataella phaffii</em> Fed-Batch Fermentations
PaperState of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
Full title: State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
PaperEffect of differential expression of pro-region on the transglutaminase productivity in pichia pastoris
Aqeel, Sahibzada Muhammad, Al‐Adeeb, Abdulqader, AL‐Ansi, Waleed, Ejaz, Sharoon, Xia, Lu, Liu, Song · 2021 · International Journal of Environment Agriculture and Biotechnology · DOI
Full title: Effect of differential expression of pro-region on the transglutaminase productivity in pichia pastoris
PaperEstablishing site-specific recombination mediated cassette exchange in Pichia pastoris and development of an optimized process for the production of mouse Tmprss2
Schinkowski, Christian · 2017 · New Trends in Mathematical Science · DOI
Full title: Establishing site-specific recombination mediated cassette exchange in Pichia pastoris and development of an optimized process for the production of mouse Tmprss2
PaperTwo-phase fed-batch modification for 48 hour peak expression of hepatitis B surface antigen in Pichia pastoris shake flask system
Tam, Yew Joon, Allaudin, Zeenathul Nazariah, Rezaei, Morvarid Akhavan, Lila, Mohd Azmi Mohd, Bahaman, Abdul, Lo, Sewn Cen, Tan, Joo Shun · 2014 · Open Life Sciences · DOI
Full title: Two-phase fed-batch modification for 48 hour peak expression of hepatitis B surface antigen in Pichia pastoris shake flask system
Authors: Tam, Yew Joon, Allaudin, Zeenathul Nazariah, Rezaei, Morvarid Akhavan, Lila, Mohd Azmi Mohd, Bahaman, Abdul, Lo, Sewn Cen, Tan, Joo Shun
PaperIdentification and Characterization of a Novel Hyperthermostable Bifunctional Cellobiohydrolase- Xylanase Enzyme for Synergistic Effect With Commercial Cellulase on Pretreated Wheat Straw Degradation
Han, Chao, Yang, Ruirui, Sun, Yanxu, Liu, Mengyu, Zhou, Lifan, Li, Duochuan · 2020 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Identification and Characterization of a Novel Hyperthermostable Bifunctional Cellobiohydrolase- Xylanase Enzyme for Synergistic Effect With Commercial Cellulase on Pretreated Wheat Straw Degradation
PaperContinuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory
Nieto‐Taype, Miguel Angel, Garcia‐Ortega, Xavier, Albiol, Joan, Montesinos, José Luis González, Valero, Francisco · 2020 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory
Authors: Nieto‐Taype, Miguel Angel, Garcia‐Ortega, Xavier, Albiol, Joan, Montesinos, José Luis González, Valero, Francisco
Year: 2020
Venue: Frontiers in Bioengineering and Biotechnology
PaperHigh-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming
Zheng, Xueyun, Zhang, Yimin, Liu, Xiaoxiao, Cheng, Li, Lin, Ying, Liang, Shuli · 2020 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming
PaperInnovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
Gasset, Arnau, Garcia‐Ortega, Xavier, Garrigós‐Martínez, Javier, Valero, Francisco, Montesinos, José Luis González · 2022 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
Authors: Gasset, Arnau, Garcia‐Ortega, Xavier, Garrigós‐Martínez, Javier, Valero, Francisco, Montesinos, José Luis González
Year: 2022
Venue: Frontiers in Bioengineering and Biotechnology
PaperPAOX1 expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determined by methanol consumption regardless of the secondary carbon source
Singh, Anamika, Narang, Atul · 2023 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: PAOX1 expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determined by methanol consumption regardless of the secondary carbon source
Authors: Singh, Anamika, Narang, Atul
Year: 2023
Venue: Frontiers in Bioengineering and Biotechnology
PaperNitrogen recovery from low-value biogenic feedstocks via steam gasification to methylotrophic yeast biomass
Shirvani, Roghayeh, Bartik, Alexander, Alves, Gustavo A. S., Hernandez, Daniel Garcia de Otazo, Müller, Stefan, Föttinger, Karin, Steiger, Matthias G. · 2023 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Nitrogen recovery from low-value biogenic feedstocks via steam gasification to methylotrophic yeast biomass
Authors: Shirvani, Roghayeh, Bartik, Alexander, Alves, Gustavo A. S., Hernandez, Daniel Garcia de Otazo, Müller, Stefan, Föttinger, Karin, Steiger, Matthias G.
Year: 2023
Venue: Frontiers in Bioengineering and Biotechnology
PaperStructural analysis and functional evaluation of the disordered ß–hexosyltransferase region from Hamamotoa (Sporobolomyces) singularis
Dagher, Suzanne F., Vaishnav, Asmita, Stanley, Christopher B., Meilleur, Flora, Edwards, Brian F.P., Bruno-Bárcena, José M. · 2023 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Structural analysis and functional evaluation of the disordered ß–hexosyltransferase region from Hamamotoa (Sporobolomyces) singularis
Authors: Dagher, Suzanne F., Vaishnav, Asmita, Stanley, Christopher B., Meilleur, Flora, Edwards, Brian F.P., Bruno-Bárcena, José M.
Year: 2023
Venue: Frontiers in Bioengineering and Biotechnology
PaperVaccine Containing the Three Allelic Variants of the Plasmodium vivax Circumsporozoite Antigen Induces Protection in Mice after Challenge with a Transgenic Rodent Malaria Parasite
Gimenez, Alba Marina, Lima, Luciana Chagas, Françoso, Kátia Sanches, Denapoli, Priscila Martins Andrade, Panatieri, Raquel Hoffmann, Bargieri, Daniel Y., Verzier, Lisa, Andolina, Chiara, Nosten, François, Rénia, Laurent, Nussenzweig, Ruth S., Nussenzweig, Victor, Amino, Rogério, Rodrigues, Maurício M., Soares, Irene S. · 2017 · Frontiers in Immunology · DOI
Full title: Vaccine Containing the Three Allelic Variants of the Plasmodium vivax Circumsporozoite Antigen Induces Protection in Mice after Challenge with a Transgenic Rodent Malaria Parasite
PaperBeauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis.
Vici, Ana C, da Cruz, Andrezza F, Facchini, Fernanda D A, de Carvalho, Caio C, Pereira, Marita G, Fonseca-Maldonado, Raquel, Ward, Richard J, Pessela, Benevides C, Fernandez-Lorente, Gloria, Torres, Fernando A G, Jorge, João A, Polizeli, Maria L T M · 2015 · Frontiers in microbiology · DOI
Full title: Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis.
Authors: Vici, Ana C, da Cruz, Andrezza F, Facchini, Fernanda D A, de Carvalho, Caio C, Pereira, Marita G, Fonseca-Maldonado, Raquel, Ward, Richard J, Pessela, Benevides C, Fernandez-Lorente, Gloria, Torres, Fernando A G, Jorge, João A, Polizeli, Maria L T M
PaperA Review of the Microbial Production of Bioactive Natural Products and Biologics
Pham, Janette V., Yilma, Mariamawit A., Feliz, Adriana, Majid, Murtadha T., Maffetone, Nicholas, Walker, Jorge R., Kim, Eunji, Cho, Hyo Je, Reynolds, Jared M., Song, Myoung‐Chong, Park, Sung Ryeol, Yoon, Yeo Joon · 2019 · Frontiers in Microbiology · DOI
Full title: A Review of the Microbial Production of Bioactive Natural Products and Biologics
Authors: Pham, Janette V., Yilma, Mariamawit A., Feliz, Adriana, Majid, Murtadha T., Maffetone, Nicholas, Walker, Jorge R., Kim, Eunji, Cho, Hyo Je, Reynolds, Jared M., Song, Myoung‐Chong, Park, Sung Ryeol, Yoon, Yeo Joon
Full title: Gene Expression, Biochemical Characterization of a sn-1, 3 Extracellular Lipase From Aspergillus niger GZUF36 and Its Model-Structure Analysis
PaperData_Sheet_1_Heterologous Expression and Characterization of a High-Efficiency Chitosanase From Bacillus mojavensis SY1 Suitable for Production of Chitosan Oligosaccharides.docx
Full title: Data_Sheet_1_Heterologous Expression and Characterization of a High-Efficiency Chitosanase From Bacillus mojavensis SY1 Suitable for Production of Chitosan Oligosaccharides.docx
PaperOverexpression and Biochemical Properties of a GH46 Chitosanase From Marine Streptomyces hygroscopicus R1 Suitable for Chitosan Oligosaccharides Preparation
Wang, Jianrong, Wang, Ping, Zhu, Mujin, Chen, Wei, Yu, Si, Zhong, Bin · 2022 · Frontiers in Microbiology · DOI
Full title: Overexpression and Biochemical Properties of a GH46 Chitosanase From Marine Streptomyces hygroscopicus R1 Suitable for Chitosan Oligosaccharides Preparation
PaperBiochemical analyses of a novel acidophilic GH5 β-mannanase from Trichoderma asperellum ND-1 and its application in mannooligosaccharides production from galactomannans
Zheng, Fengzhen, Basit, Abdul, Wang, Jiaqiang, Zhuang, Huan, Chen, Jun, Jian-fen, Zhang · 2023 · Frontiers in Microbiology · DOI
Full title: Biochemical analyses of a novel acidophilic GH5 β-mannanase from Trichoderma asperellum ND-1 and its application in mannooligosaccharides production from galactomannans
PaperFrom linoleic acid to hexanal and hexanol by whole cell catalysis with a lipoxygenase, hydroperoxide lyase and reductase cascade in Komagataella phaffii
Hashem, Chiam, Hochrinner, Julius, Bürgler, Moritz B., Rinnofner, Claudia, Pichler, Harald, Winkler, Margit · 2022 · Frontiers in Molecular Biosciences · DOI
Full title: From linoleic acid to hexanal and hexanol by whole cell catalysis with a lipoxygenase, hydroperoxide lyase and reductase cascade in Komagataella phaffii
Authors: Hashem, Chiam, Hochrinner, Julius, Bürgler, Moritz B., Rinnofner, Claudia, Pichler, Harald, Winkler, Margit
PaperPreparative Production of Functionalized (N- and O-Heterocyclic) Polycyclic Aromatic Hydrocarbons by Human Cytochrome P450 3A4 in a Bioreactor
Srdič, Matic, Fessner, Nico D., Yıldız, Deniz, Glieder, Anton, Spiertz, Markus, Schwaneberg, Ulrich · 2022 · Biomolecules · DOI
Full title: Preparative Production of Functionalized (N- and O-Heterocyclic) Polycyclic Aromatic Hydrocarbons by Human Cytochrome P450 3A4 in a Bioreactor
PaperSpecific Phenylpropanoid Oligomerization in a Neutral Environment by the Recombinant Alkaline Laccase from Paramyrothecium roridum VKM F-3565.
Renfeld, Zhanna V, Chernykh, Alexey M, Gorina, Sofia Yu, Baskunov, Boris P, Moiseeva, Olga V, Trachtmann, Natalia V, Validov, Shamil Z, Kolomytseva, Marina P · 2025 · Biomolecules · DOI
Full title: Specific Phenylpropanoid Oligomerization in a Neutral Environment by the Recombinant Alkaline Laccase from Paramyrothecium roridum VKM F-3565.
Authors: Renfeld, Zhanna V, Chernykh, Alexey M, Gorina, Sofia Yu, Baskunov, Boris P, Moiseeva, Olga V, Trachtmann, Natalia V, Validov, Shamil Z, Kolomytseva, Marina P
PaperStatistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris
Maity, Nitu, Mishra, Saroj · 2019 · Biomolecules · DOI
Full title: Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris
PaperExpression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi
Birchfield, Aaron S., McIntosh, Cecilia A. · 2024 · BioTech · DOI
Full title: Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi
Authors: Birchfield, Aaron S., McIntosh, Cecilia A.
PaperOptimization of Bioethanol In Silico Production Process in a Fed-Batch Bioreactor Using Non-Linear Model Predictive Control and Evolutionary Computation Techniques
Freitas, Hanniel Ferreira Sarmento de, Olivo, José Eduardo, Andrade, Cid Marcos Gonçalves · 2017 · Energies · DOI
Full title: Optimization of Bioethanol In Silico Production Process in a Fed-Batch Bioreactor Using Non-Linear Model Predictive Control and Evolutionary Computation Techniques
Authors: Freitas, Hanniel Ferreira Sarmento de, Olivo, José Eduardo, Andrade, Cid Marcos Gonçalves
PaperPerformance of Recombinant Komagataella phaffii in Plant-Based Meat Flavor Compound-Leghemoglobin (LegH) Production through Fed-Batch Fermentations
Full title: Performance of Recombinant Komagataella phaffii in Plant-Based Meat Flavor Compound-Leghemoglobin (LegH) Production through Fed-Batch Fermentations
PaperState of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
Full title: State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
PaperEnhancing Antimicrobial Peptide Productivity in Pichia pastoris (Muts Strain) by Improving the Fermentation Process Based on Increasing the Volumetric Methanol Consumption Rate
Full title: Enhancing Antimicrobial Peptide Productivity in Pichia pastoris (Muts Strain) by Improving the Fermentation Process Based on Increasing the Volumetric Methanol Consumption Rate
PaperDevelopment of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain Using Pichia pastoris in Stirred-Tank Bioreactor
Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, Florencia, Wetzler, Diana E., Gentili, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas · 2023 · Fermentation · DOI
Full title: Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain Using Pichia pastoris in Stirred-Tank Bioreactor
Authors: Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, Florencia, Wetzler, Diana E., Gentili, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas
PaperLipase from Yarrowia lipolytica: Prospects as an Industrial Biocatalyst for Biotechnological Applications
Silva, Jessica Lopes da, Sales, Misael Bessa, Bizerra, Viviane de Castro, Nobre, Millena Mara Rabelo, Braz, Ana Kátia de Sousa, Sousa, Patrick da Silva, Cavalcante, Antônio Luthierre Gama, Melo, Rafael Leandro Fernandes, Sousa, Paulo Gonçalves de, Neto, Francisco Simão, Fonseca, Aluísio Marques da, Santos, José Cleiton Sousa dos · 2023 · Fermentation · DOI
Full title: Lipase from Yarrowia lipolytica: Prospects as an Industrial Biocatalyst for Biotechnological Applications
Authors: Silva, Jessica Lopes da, Sales, Misael Bessa, Bizerra, Viviane de Castro, Nobre, Millena Mara Rabelo, Braz, Ana Kátia de Sousa, Sousa, Patrick da Silva, Cavalcante, Antônio Luthierre Gama, Melo, Rafael Leandro Fernandes, Sousa, Paulo Gonçalves de, Neto, Francisco Simão, Fonseca, Aluísio Marques da, Santos, José Cleiton Sousa dos
PaperTranscriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3
Full title: Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3
PaperOptimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endothelial Growth Factor165 and Programmed Death-1 in Pichia pastoris and Evaluation of Antitumor Activity In Vivo
Xiong, Chenghao, Mao, Yingqing, Wu, Tao, Kang, Nannan, Zhao, Mingjun, Di, Rongrong, Li, Xiaoping, Ji, Xuemei, Liu, Yu · 2018 · International Journal of Molecular Sciences · DOI
Full title: Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endothelial Growth Factor165 and Programmed Death-1 in Pichia pastoris and Evaluation of Antitumor Activity In Vivo
PaperHeterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
Zadorozhny, A. V., Ushakov, Viktor Sergeevich, Розанов, А. С., Bogacheva, Natalia Vladimirovna, Shlyakhtun, Valeria Nikolayevna, Voskoboev, Mikhail Evgenyevich, Korzhuk, Anton V., Romancev, Vladislav Anatolevich, Bannikova, Svetlana V., Mescheryakova, Irina Anatolyevna, Антонов, Е. В., Vasilieva, Asya R., Pavlova, Elena Iurevna, Chesnokov, Danil Olegovich, Shedko, Elizaveta Dmitrievna, Bryanskaya, A. V., Bochkov, D. V., Goryachkovskaya, T. N., Peltek, S. E. · 2022 · International Journal of Molecular Sciences · DOI
Full title: Heterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
Authors: Zadorozhny, A. V., Ushakov, Viktor Sergeevich, Розанов, А. С., Bogacheva, Natalia Vladimirovna, Shlyakhtun, Valeria Nikolayevna, Voskoboev, Mikhail Evgenyevich, Korzhuk, Anton V., Romancev, Vladislav Anatolevich, Bannikova, Svetlana V., Mescheryakova, Irina Anatolyevna, Антонов, Е. В., Vasilieva, Asya R., Pavlova, Elena Iurevna, Chesnokov, Danil Olegovich, Shedko, Elizaveta Dmitrievna, Bryanskaya, A. V., Bochkov, D. V., Goryachkovskaya, T. N., Peltek, S. E.
Year: 2022
Venue: International Journal of Molecular Sciences
PaperA New Expression System Based on Psychrotolerant Debaryomyces macquariensis Yeast and Its Application to the Production of Cold-Active β-d-Galactosidase from Paracoccus sp. 32d
Full title: A New Expression System Based on Psychrotolerant Debaryomyces macquariensis Yeast and Its Application to the Production of Cold-Active β-d-Galactosidase from Paracoccus sp. 32d
PaperEnhancing the Heterologous Expression of a Thermophilic Endoglucanase and Its Cost-Effective Production in Pichia pastoris Using Multiple Strategies
Dai, Wuling, Dong, Haofan, Zhang, Zhaokun, Wu, Xin, Bao, Tongtong, Gao, Le, Chen, Xiaoyi · 2023 · International Journal of Molecular Sciences · DOI
Full title: Enhancing the Heterologous Expression of a Thermophilic Endoglucanase and Its Cost-Effective Production in Pichia pastoris Using Multiple Strategies
PaperProduction of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unveiling Antimicrobial and Anticancer Functionalities.
Yen, Chih-Ching, Wu, Pei-Ying, Ou-Yang, Huan, Chen, Hsiao-Ling, Chong, Kowit-Yu, Chang, Ro-Lin, Chen, Chuan-Mu · 2024 · International journal of molecular sciences · DOI
Full title: Production of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unveiling Antimicrobial and Anticancer Functionalities.
PaperAn Interplay between Transcription Factors and Recombinant Protein Synthesis in Yarrowia lipolytica at Transcriptional and Functional Levels—The Global View
Gorczyca, Maria, Korpys-Woźniak, Paulina, Celińska, Ewelina · 2024 · International Journal of Molecular Sciences · DOI
Full title: An Interplay between Transcription Factors and Recombinant Protein Synthesis in Yarrowia lipolytica at Transcriptional and Functional Levels—The Global View
PaperHeterologous Production, Purification and Characterization of Two Cold-Active β-d-Galactosidases with Transglycosylation Activity from the Psychrotolerant Arctic Bacterium Arthrobacter sp. S3* Isolated from Spitsbergen Island Soil.
Wanarska, Marta, Pawlak-Szukalska, Anna, Rosińska, Aleksandra, Kozłowska-Tylingo, Katarzyna · 2024 · International journal of molecular sciences · DOI
Full title: Heterologous Production, Purification and Characterization of Two Cold-Active β-d-Galactosidases with Transglycosylation Activity from the Psychrotolerant Arctic Bacterium Arthrobacter sp. S3* Isolated from Spitsbergen Island Soil.
Authors: Wanarska, Marta, Pawlak-Szukalska, Anna, Rosińska, Aleksandra, Kozłowska-Tylingo, Katarzyna
Year: 2024
Venue: International journal of molecular sciences
PaperSustainability Assessment in Recombinant Human Insulin Production—Evaluating the Environmental Impacts of Microbial Growth Medium Components and Formulations
Chatzipanagiotou, Konstantina‐Roxani, Pappas, Alex G., Petrakli, Foteini, Antonaropoulos, George, Koumoulos, Elias P. · 2025 · Journal of Experimental and Theoretical Analyses · DOI
Full title: Sustainability Assessment in Recombinant Human Insulin Production—Evaluating the Environmental Impacts of Microbial Growth Medium Components and Formulations
Authors: Chatzipanagiotou, Konstantina‐Roxani, Pappas, Alex G., Petrakli, Foteini, Antonaropoulos, George, Koumoulos, Elias P.
Year: 2025
Venue: Journal of Experimental and Theoretical Analyses
PaperQuantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris
Jordà, Joel, Rojas, Hugo, Carnicer, Marc, Wahl, A., Ferrer, Pau, Albiol, Joan · 2014 · Metabolites · DOI
Full title: Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris
Authors: Jordà, Joel, Rojas, Hugo, Carnicer, Marc, Wahl, A., Ferrer, Pau, Albiol, Joan
PaperKomagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Хлебодарова, Т. М., Bogacheva, N. V., Zadorozhny, A. V., Bryanskaya, A. V., Vasilieva, Asya R., Chesnokov, Danil Olegovich, Pavlova, Elena I., Peltek, S. E. · 2024 · Microorganisms · DOI
Full title: Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Authors: Хлебодарова, Т. М., Bogacheva, N. V., Zadorozhny, A. V., Bryanskaya, A. V., Vasilieva, Asya R., Chesnokov, Danil Olegovich, Pavlova, Elena I., Peltek, S. E.
ThesisEvaluation of product distribution in chemostat and batch fermentation in lactic acid-producing komagataella phaffii strains utilizing glycerol as substrate
Full title: Evaluation of product distribution in chemostat and batch fermentation in lactic acid-producing komagataella phaffii strains utilizing glycerol as substrate
Full title: Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso
ThesisHigh-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
Blanchard, Véronique; Gadkari, R.P; George, A.V.E. et al. · 2008 · Theses · DOI
Full title: High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
ThesisComparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain
Brady, Joseph R; Whittaker, Charles A; Tan, Melody C et al. · 2021 · Theses · DOI
Full title: Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain
Authors: Brady, Joseph R; Whittaker, Charles A; Tan, Melody C; Kristensen, D Lee; Ma, Duanduan; Dalvie, Neil C; Love, Kerry Routenberg; Love, J Christopher
ThesisCRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Dalvie, Neil C.; Lorgeree, Timothy R.; Yang, Yuchen et al. · 2024 · Theses · DOI
Full title: CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Authors: Dalvie, Neil C.; Lorgeree, Timothy R.; Yang, Yuchen; Rodriguez-Aponte, Sergio A.; Whittaker, Charles A.; Hinckley, Joshua A.; Clark, John J.; Del Rosario, Amanda M.; Love, Kerry R.; Love, J. C.
ThesisDevelopment of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
Full title: Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
ThesisProcess optimization and scale-up for the production of a diagnostic monoclonal antibody against congenital adrenal hyperplasia (CAH) : a thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Bioprocess Engineering at Massey University, Manawatu Campus, New Zealand
Full title: Process optimization and scale-up for the production of a diagnostic monoclonal antibody against congenital adrenal hyperplasia (CAH) : a thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Bioprocess Engineering at Massey University, Manawatu Campus, New Zealand
Full title: Kinetic study of fed-batch and continuous bioconversion processes for L-Phenylacetylcarbinol (L-PAC) production by the yeast Candida utilis
ThesisModel-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter
Barrigón de San Marcos, José Manuel · 2015 · Theses · DOI
Full title: Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter
ThesisUtilización de glicerol proveniente de la industria de biodiesel como fuente de carbono para la producción de lipasas recombinantes en Pichia pastoris
Full title: Utilización de glicerol proveniente de la industria de biodiesel como fuente de carbono para la producción de lipasas recombinantes en Pichia pastoris
ThesisA step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches
Full title: A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches
ThesisL’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic
Full title: L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic
Full title: Estratègies d’operació per a la producció de lipases de Rhizopus oryzae amb el fenotip Mut(s) de Pichia pastoris mitjançant substrats mixtes
ThesisCombining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris
Full title: Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris
Full title: Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris
ThesisPhysiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess
Full title: Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess
PaperRecombinant expression and characterization of an endoglucanase III (cel12a) from Trichoderma harzianum (Hypocreaceae) in the yeast Pichia pastoris
Generoso, Wesley Cardoso, Malagó-Jr, Wilson, Pereira, Nei, Henrique‐Silva, Flávio · 2012 · Genetics and Molecular Research · DOI
Full title: Recombinant expression and characterization of an endoglucanase III (cel12a) from Trichoderma harzianum (Hypocreaceae) in the yeast Pichia pastoris
PaperHigh-yield intracellular production of an exo-polygalacturonase enzyme via heterologous expression of Penicillium notatum gene in Saccharomyces cerevisiae
Zhang, Shuangshuang, Amin, Faiza, Xiong, Mingyong, Bhatti, Haq Nawaz, Bilal, Muhammad · 2021 · International Food Research Journal · DOI
Full title: High-yield intracellular production of an exo-polygalacturonase enzyme via heterologous expression of Penicillium notatum gene in Saccharomyces cerevisiae
Authors: Zhang, Shuangshuang, Amin, Faiza, Xiong, Mingyong, Bhatti, Haq Nawaz, Bilal, Muhammad
PaperDetermination of Putative Vacuolar Proteases, PEP4 and PRB1 in a Novel Yeast Expression Host Meyerozyma guilliermondii Strain SO Using Bioinformatics Tools
Lorrine, Okojie Eseoghene, Rahman, Raja Noor Zaliha Raja Abd, Tan, Joo Shun, Khairuddin, Raja Farhana Raja, Salleh, Abu Bakar, Oslan, Siti Nurbaya · 2022 · Pertanika journal of science & technology · DOI
Full title: Determination of Putative Vacuolar Proteases, PEP4 and PRB1 in a Novel Yeast Expression Host Meyerozyma guilliermondii Strain SO Using Bioinformatics Tools
Authors: Lorrine, Okojie Eseoghene, Rahman, Raja Noor Zaliha Raja Abd, Tan, Joo Shun, Khairuddin, Raja Farhana Raja, Salleh, Abu Bakar, Oslan, Siti Nurbaya
PaperIsolation of the Astacin-like metalloprotease coding gene (astl) and assessment of its insecticidal activity towards Spodoptera littoralis and Sitophilus oryzae
Diab, Mervat R., Ahmed, Mahmoud M., Hussein, Ebtissam H. A., Mohammed, Ahmed, Adams, M, Venema, Herold, V, Astrin, J, Hfer, H, Spelda, J, Holsteinj, Bayer, S, Hendrich, L, Huber, B, Kielhorn, K, Krammer, H, Lemke, M, Carlosmonje, J, Morinire, J, Rulik, B, Petersen, M, Janssen, H, Muster, C, Atia, Mam, Osman, G, Elmenofy, W, Atkinson, R, Vonarx, E, Howden, M, Barbaro, K, Knysak, I, Martins, R, Hogan, C, Winkel, K, Bloomquist, J, Bond, J, Beynon, R J, Chandler, D, Bailey, A, Tatchell, G, Davidson, G, Greaves, J, Grant, W, Silveira, Da, Filho, R, Mangili, Jfs, Veiga, O, Gremski, S, Nader, W, Dietrich, H, C, Silveira, Da, Wille, R, Chaim, Acm, Appel, O, Silva, M, Franco, D, Toma, Crc, Mangili, L, Gremski, O, Dietrich, W, Nader, C, Veiga, H, S, Derksen, A, Granados, R, Dumermuth, E, Sterchi, E, Jiang, W, Wolz, R, Bond, J S, Flannery, A, Beynon, R, Escoubas, P, Sollod, B, King, G, Feitosa, L, Gremski, W, Veiga, S, Elias, Mcqb, Graner, E, Mangili, O, Brentani, R, Fitches, E, Pyati, P, King, G, Gatehouse, J, Fletcher, J, Smith, R, Donoghue, O', Nilges, S, Connor, M, Howden, M, Christie, M, King, M, G, Futrell, J, Gomis-Rth, F, Stcker, Trillo-Muyo S, W · 2021 · Jordan Journal of Biological Sciences · DOI
Full title: Isolation of the Astacin-like metalloprotease coding gene (astl) and assessment of its insecticidal activity towards Spodoptera littoralis and Sitophilus oryzae
Authors: Diab, Mervat R., Ahmed, Mahmoud M., Hussein, Ebtissam H. A., Mohammed, Ahmed, Adams, M, Venema, Herold, V, Astrin, J, Hfer, H, Spelda, J, Holsteinj, Bayer, S, Hendrich, L, Huber, B, Kielhorn, K, Krammer, H, Lemke, M, Carlosmonje, J, Morinire, J, Rulik, B, Petersen, M, Janssen, H, Muster, C, Atia, Mam, Osman, G, Elmenofy, W, Atkinson, R, Vonarx, E, Howden, M, Barbaro, K, Knysak, I, Martins, R, Hogan, C, Winkel, K, Bloomquist, J, Bond, J, Beynon, R J, Chandler, D, Bailey, A, Tatchell, G, Davidson, G, Greaves, J, Grant, W, Silveira, Da, Filho, R, Mangili, Jfs, Veiga, O, Gremski, S, Nader, W, Dietrich, H, C, Silveira, Da, Wille, R, Chaim, Acm, Appel, O, Silva, M, Franco, D, Toma, Crc, Mangili, L, Gremski, O, Dietrich, W, Nader, C, Veiga, H, S, Derksen, A, Granados, R, Dumermuth, E, Sterchi, E, Jiang, W, Wolz, R, Bond, J S, Flannery, A, Beynon, R, Escoubas, P, Sollod, B, King, G, Feitosa, L, Gremski, W, Veiga, S, Elias, Mcqb, Graner, E, Mangili, O, Brentani, R, Fitches, E, Pyati, P, King, G, Gatehouse, J, Fletcher, J, Smith, R, Donoghue, O', Nilges, S, Connor, M, Howden, M, Christie, M, King, M, G, Futrell, J, Gomis-Rth, F, Stcker, Trillo-Muyo S, W
PaperIsolation, characterization, and molecular cloning of the cDNA encoding a novel phytase from Aspergillus niger 113 and high expression in Pichia pastoris.
Xiong, Ai-Sheng, Yao, Quan-Hong, Peng, Ri-He, Li, Xian, Fan, Hui-Qin, Guo, Mei-Jin, Zhang, Si-Liang · 2004 · Journal of biochemistry and molecular biology · DOI
Full title: Isolation, characterization, and molecular cloning of the cDNA encoding a novel phytase from Aspergillus niger 113 and high expression in Pichia pastoris.
PaperOvercoming Obstacles in Protein Expression in the Yeast Pichia pastoris: Interviews of Leaders in the Pichia Field.
Zoe Ingram, Abhay H Patkar, Dahoon Oh, Kevin Zhang, Christina Chung, Joan Lin-Cereghino, Geoff P. Lin-Cereghino · 2021 · Pacific journal of health · DOI
Full title: Overcoming Obstacles in Protein Expression in the Yeast Pichia pastoris: Interviews of Leaders in the Pichia Field.
Authors: Zoe Ingram, Abhay H Patkar, Dahoon Oh, Kevin Zhang, Christina Chung, Joan Lin-Cereghino, Geoff P. Lin-Cereghino
PaperConformational Bias in 2'-Selenium-Modified Nucleosides and the Effect on Helical Structure and Extracellular Recombinant Protein Production: Current Systems and Applications
Thompson, R. Adam · 2022 · Counseling And Psychological Services Dissertations (Georgia State University) · DOI
Full title: Conformational Bias in 2'-Selenium-Modified Nucleosides and the Effect on Helical Structure and Extracellular Recombinant Protein Production: Current Systems and Applications
Authors: Thompson, R. Adam
Year: 2022
Venue: Counseling And Psychological Services Dissertations (Georgia State University)
PaperDevelopment of simple kinetic models and parameter estimation for simulation of recombinant human serum albumin production by Pichia pastoris
Panchiga, Chongchittapiban, Jӧrgen, Borg, Waiprib, Yaowapha, Jindarat, Pimsamarn, Anan, Tongta · 2016 · AFRICAN JOURNAL OF BIOTECHNOLOGY · DOI
Full title: Development of simple kinetic models and parameter estimation for simulation of recombinant human serum albumin production by Pichia pastoris
PaperCellobiohydrolase B of Aspergillus niger over-expressed in Pichia pastoris stimulates hydrolysis of oil palm empty fruit bunches.
Woon, James Sy-Keen, Mackeen, Mukram M, Illias, Rosli M, Mahadi, Nor M, Broughton, William J, Murad, Abdul Munir Abdul, Abu Bakar, Farah Diba · 2017 · PeerJ · DOI
Full title: Cellobiohydrolase B of Aspergillus niger over-expressed in Pichia pastoris stimulates hydrolysis of oil palm empty fruit bunches.
Authors: Woon, James Sy-Keen, Mackeen, Mukram M, Illias, Rosli M, Mahadi, Nor M, Broughton, William J, Murad, Abdul Munir Abdul, Abu Bakar, Farah Diba
PaperA central composite design to investigate antibody fragment production by Pichia pastoris
Chan, Joyce Y.C. · 2006 · DSpace@MIT (Massachusetts Institute of Technology)
Full title: A central composite design to investigate antibody fragment production by Pichia pastoris
Authors: Chan, Joyce Y.C.
Year: 2006
Venue: DSpace@MIT (Massachusetts Institute of Technology)
PaperAn upstream platform for the production of high grade heterologous proteins in the yeast Pichia pastoris
S. Woodhouse · 2016 · Doctoral thesis, UCL (University College London).
Full title: An upstream platform for the production of high grade heterologous proteins in the yeast Pichia pastoris
Authors: S. Woodhouse
Year: 2016
Venue: Doctoral thesis, UCL (University College London).
PaperAQUAPORINS: Production Optimization and Characterization
Öberg, Fredrik · 2011 · Gothenburg University Publications Electronic Archive (Gothenburg University)
Full title: AQUAPORINS: Production Optimization and Characterization
Authors: Öberg, Fredrik
Year: 2011
Venue: Gothenburg University Publications Electronic Archive (Gothenburg University)
PaperBioprospecting the Regional Diversity of Australian Wine Microbiota
Hartmann, Lisa Anne · 2020 · Adelaide Research & Scholarship (AR&S) (University of Adelaide)
Full title: Bioprospecting the Regional Diversity of Australian Wine Microbiota
Authors: Hartmann, Lisa Anne
Year: 2020
Venue: Adelaide Research & Scholarship (AR&S) (University of Adelaide)
PaperCharacterization and Analysis of Proteins Secreted by the Mutant Pichia pastoris Strain, bgs13
Naranjo, Christopher A. · 2019 · Scholarly Commons (University of the Pacific)
Full title: Characterization and Analysis of Proteins Secreted by the Mutant Pichia pastoris Strain, bgs13
Authors: Naranjo, Christopher A.
Year: 2019
Venue: Scholarly Commons (University of the Pacific)
PaperCharacterization of non-conventional yeasts under hyperbaric conditions : cellular response to oxidative stress
Lopes, Marlene · 2013 · RepositóriUM (Universidade do Minho)
Full title: Characterization of non-conventional yeasts under hyperbaric conditions : cellular response to oxidative stress
Authors: Lopes, Marlene
Year: 2013
Venue: RepositóriUM (Universidade do Minho)
PaperCloning, Expression, and Biochemical Characterization of Recombinant Putative Glucosyltransferases Clone 3 and 8 from Grapefruit (Citrus paradisi)
Hayford, Deborah · 2012 · Digital Commons - East Tennessee State University (East Tennessee State University)
Full title: Cloning, Expression, and Biochemical Characterization of Recombinant Putative Glucosyltransferases Clone 3 and 8 from Grapefruit (Citrus paradisi)
Authors: Hayford, Deborah
Year: 2012
Venue: Digital Commons - East Tennessee State University (East Tennessee State University)
PaperCloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farnaz Emamdoust
Farnaz, Emamdoust · 2018
Full title: Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farnaz Emamdoust
Authors: Farnaz, Emamdoust
Year: 2018
PaperCloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris
Carty, Charlene · 2003
Full title: Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris
Authors: Carty, Charlene
Year: 2003
PaperCombined Fermentation and Recovery Using Expanded Bed Chromatography
Cochran, Keith Jacob · 2006 · Digital WPI
Full title: Combined Fermentation and Recovery Using Expanded Bed Chromatography
Authors: Cochran, Keith Jacob
Year: 2006
Venue: Digital WPI
PaperComparison of two codon optimization strategies enhancing recombinant Sus scrofa lysozyme production in Pichia pastoris.
D. Zhu, G. Cai, D. Wu, J. Lu · 2015 · Cellular and Molecular Biology
Full title: Comparison of two codon optimization strategies enhancing recombinant Sus scrofa lysozyme production in Pichia pastoris.
Authors: D. Zhu, G. Cai, D. Wu, J. Lu
Year: 2015
Venue: Cellular and Molecular Biology
PaperDesign of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Monteiro, Diogo José Pinheiro · 2018 · UBibliorum repositorio digital da ubi (University of Beira Interior)
Full title: Design of an one-step platform purification of STEAP1 using Hydrophobic Interaction Chromatography
Authors: Monteiro, Diogo José Pinheiro
Year: 2018
Venue: UBibliorum repositorio digital da ubi (University of Beira Interior)
PaperDesign of an optimised fed-batch process for insulin precursor production in Pichia pastoris
Bhardwaj, Vinayak · 2011 · Belarusian State Pedagogical University repository (Belarusian State Pedagogical University)
Full title: Design of an optimised fed-batch process for insulin precursor production in Pichia pastoris
Authors: Bhardwaj, Vinayak
Year: 2011
Venue: Belarusian State Pedagogical University repository (Belarusian State Pedagogical University)
PaperDeveloping strategies for systems metabolic engineering of Pichia pastoris
Tomàs‐Gamisans, Màrius · 2017 · TDX (Tesis Doctorals en Xarxa)
Full title: Developing strategies for systems metabolic engineering of Pichia pastoris
Authors: Tomàs‐Gamisans, Màrius
Year: 2017
Venue: TDX (Tesis Doctorals en Xarxa)
PaperDevelopment and Application of Advanced Synthetic Biology Tools for Engineering Chemical Production in Yarrowia lipolytica
Schwartz, Cory · 2018 · eScholarship (California Digital Library)
Full title: Development and Application of Advanced Synthetic Biology Tools for Engineering Chemical Production in Yarrowia lipolytica
Authors: Schwartz, Cory
Year: 2018
Venue: eScholarship (California Digital Library)
PaperDevelopment of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition (SCADA) Software for Automation of High Cell Density Pichia pastoris Fermentations in a 30L Bioreactor.
Choe, Kisurb · 2017 · NCSU Libraries Repository (North Carolina State University Libraries)
Full title: Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition (SCADA) Software for Automation of High Cell Density Pichia pastoris Fermentations in a 30L Bioreactor.
Authors: Choe, Kisurb
Year: 2017
Venue: NCSU Libraries Repository (North Carolina State University Libraries)
PaperDevelopment of methods for the production and purification of streptavidin from Streptomyces avidinii and heterologous hosts
Müller, Jakob · 2016
Full title: Development of methods for the production and purification of streptavidin from Streptomyces avidinii and heterologous hosts
Authors: Müller, Jakob
Year: 2016
PaperDevelopment of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Wang, Baolong · 2019 · UCL Discovery (University College London)
Full title: Development of New Cultivation Strategies to Enhance Heterologous Protein Production in Pichia Pastoris
Authors: Wang, Baolong
Year: 2019
Venue: UCL Discovery (University College London)
PaperDevelopment of Pichia pastoris as a production system for HPV16 L1 virus-like particles as component to a subunit vaccine
Full title: Development of Pichia pastoris as a production system for HPV16 L1 virus-like particles as component to a subunit vaccine
Authors: Kotze, Lara
Year: 2007
Venue: SUNScholar (Stellenbosch University)
PaperDevelopment of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
Manderson, Daniel · 2004
Full title: Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
Authors: Manderson, Daniel
Year: 2004
PaperDevelopment of Saccharomyces cerevisiae as a recombinant antibody factory
Ruijter, Jorg C. de, Jorg, C · 2016 · Aaltodoc (Aalto University)
Full title: Development of Saccharomyces cerevisiae as a recombinant antibody factory
Authors: Ruijter, Jorg C. de, Jorg, C
Year: 2016
Venue: Aaltodoc (Aalto University)
PaperEffect of culture conditions on recombinant protein production in Chlamydomonas reinhardtii
Venue: Sheng wu gong cheng xue bao = Chinese journal of biotechnology
PaperExpression of the Caenorhabditis elegans aryl hydrocarbon receptor ligand binding domain
Helaly, Ahmed · 2011 · Nottingham ePrints (University of Nottingham)
Full title: Expression of the Caenorhabditis elegans aryl hydrocarbon receptor ligand binding domain
Authors: Helaly, Ahmed
Year: 2011
Venue: Nottingham ePrints (University of Nottingham)
PaperExpression of the Rhizopus oryzae lipase in Pichia pastoris under the control of the FLD1 promoter
D. Rodriguez · 2007
Full title: Expression of the Rhizopus oryzae lipase in Pichia pastoris under the control of the FLD1 promoter
Authors: D. Rodriguez
Year: 2007
PaperExpression, purification and characterization of fungal and viral recombinant proteins
Vihinen, Helena · 2001 · Aaltodoc (Aalto University)
Full title: Expression, purification and characterization of fungal and viral recombinant proteins
Authors: Vihinen, Helena
Year: 2001
Venue: Aaltodoc (Aalto University)
PaperExpression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris
Le, Henry H. · 2019 · Scholarly Commons (University of the Pacific)
Full title: Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris
Authors: Le, Henry H.
Year: 2019
Venue: Scholarly Commons (University of the Pacific)
PaperFarklı çimento tipleri ile priz hızlandırıcı katkıların etkinliliği.
Tuğba, Üstüner, Didem · 2009 · New Trends in Mathematical Science
Full title: Farklı çimento tipleri ile priz hızlandırıcı katkıların etkinliliği.
Authors: Tuğba, Üstüner, Didem
Year: 2009
Venue: New Trends in Mathematical Science
Paper[Feeding of mixed-carbon-resource during the expression phase in cultivation of recombinant Pichia pastoris expressing angiostatin].
Xie, Jing-Li, Zhou, Qing-Wei, Zhang, Li, Ye, Qin, Xin, Li, Du, Peng, Gan, Ren-Bao · 2003 · Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Full title: [Feeding of mixed-carbon-resource during the expression phase in cultivation of recombinant Pichia pastoris expressing angiostatin].
Authors: Xie, Jing-Li, Zhou, Qing-Wei, Zhang, Li, Ye, Qin, Xin, Li, Du, Peng, Gan, Ren-Bao
Year: 2003
Venue: Sheng wu gong cheng xue bao = Chinese journal of biotechnology
PaperFermentation Condition for the Expression of Human Lysozyme by Pichia pastoris SMD1168
Xiaoting Jie · 2003
Full title: Fermentation Condition for the Expression of Human Lysozyme by Pichia pastoris SMD1168
Authors: Xiaoting Jie
Year: 2003
PaperHeterologous protein production in Kluyveromyces lactis : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biotechnology and Bioprocess Engineering at Massey University, Palmerston North, New Zealand
Russell, Carolyn Margaret · 1993
Full title: Heterologous protein production in Kluyveromyces lactis : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biotechnology and Bioprocess Engineering at Massey University, Palmerston North, New Zealand
Authors: Russell, Carolyn Margaret
Year: 1993
Paper[High level expression, purification and characterization of human kallikrein-1 in Pichia pastoris].
Huang, Xiudong, Wang, Shusheng, Chen, Peixin, Wang, Jun, Chen, Yaoguo, Pan, Xuegong, Cao, Zhifang · 2008 · Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Full title: [High level expression, purification and characterization of human kallikrein-1 in Pichia pastoris].
Full title: Human Aquaporins: Production, Characterization and Interactions
Authors: Sjöhamn, Jennie
Year: 2015
Venue: Gothenburg University Publications Electronic Archive (Gothenburg University)
Paperİki farklı metabolik mühendislik yaklaşımıyla geliştirilen Pichia pastoris hücrelerinin insan büyüme hormonu üretim performanslarının karşılaştırılması.
Gül, Zerze, Halime · 2012 · New Trends in Mathematical Science
Full title: İki farklı metabolik mühendislik yaklaşımıyla geliştirilen Pichia pastoris hücrelerinin insan büyüme hormonu üretim performanslarının karşılaştırılması.
Authors: Gül, Zerze, Halime
Year: 2012
Venue: New Trends in Mathematical Science
PaperImproved Production And Secretion Of Cancer Testis Antigen Ssx2 In Different Expression Hosts
Huang, Chung Kai · 2009 · eCommons (Cornell University)
Full title: Improved Production And Secretion Of Cancer Testis Antigen Ssx2 In Different Expression Hosts
Authors: Huang, Chung Kai
Year: 2009
Venue: eCommons (Cornell University)
PaperIn-Situ Formation Of Biodegradable Polyhydroxybutyrate Polymer For Targeted Occlusion Of Solid Tumor Neovasculature
Parker, Stephanie A. · 2015 · eCommons (Cornell University)
Full title: In-Situ Formation Of Biodegradable Polyhydroxybutyrate Polymer For Targeted Occlusion Of Solid Tumor Neovasculature
Authors: Parker, Stephanie A.
Year: 2015
Venue: eCommons (Cornell University)
PaperIn situ product recovery of antibodies with a reverse flow diafiltration membrane bioreactor
Full title: Manufacturing biopharmaceutical proteins by transient expression in Nicotiana tabacum (L.)
Authors: Buyel, Johannes F., Fischer, Rainer
Year: 2013
Venue: RWTH Publications (RWTH Aachen)
PaperMetabolic modelling and 13C flux analysis application to biotechnologically important yeasts and a fungus
Jouhten, Paula · 2009 · Aaltodoc (Aalto University)
Full title: Metabolic modelling and 13C flux analysis application to biotechnologically important yeasts and a fungus
Authors: Jouhten, Paula
Year: 2009
Venue: Aaltodoc (Aalto University)
PaperMetabolic profiling of Meyerozyma guilliermondii strain SO and a recombinant strain SO2 expressing lipase
Fam, Jye Ping · 2018 · Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia)
Full title: Metabolic profiling of Meyerozyma guilliermondii strain SO and a recombinant strain SO2 expressing lipase
Authors: Fam, Jye Ping
Year: 2018
Venue: Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia)
PaperMethods for production of recombinant enzymes for collagen degradation
Eriksson, Tea, Su, Elin · 2020 · Lund University Publications Student Papers (Lund University)
Full title: Methods for production of recombinant enzymes for collagen degradation
Authors: Eriksson, Tea, Su, Elin
Year: 2020
Venue: Lund University Publications Student Papers (Lund University)
PaperMolecular cloning, expression and characterization of two potential antibacterial fusion hydrophobins in Pichia pastoris
Jiang, Xinyu · 2015 · Työväentutkimus Vuosikirja
Full title: Molecular cloning, expression and characterization of two potential antibacterial fusion hydrophobins in Pichia pastoris
Authors: Jiang, Xinyu
Year: 2015
Venue: Työväentutkimus Vuosikirja
PaperNovel approaches to target infectious diseases : the utility of Auger electrons and scFvs for imaging and control
Rathje, Claudia Cattoni · 2014 · Kent Academic Repository (University of Kent)
Full title: Novel approaches to target infectious diseases : the utility of Auger electrons and scFvs for imaging and control
Authors: Rathje, Claudia Cattoni
Year: 2014
Venue: Kent Academic Repository (University of Kent)
PaperOksijen aktarım koşullarının pichia pastoris ile gliseraldehit-3-fosfat dehidrojenaz promotörü altında rekombinant glukoz izomeraz üretimine etkisi.
Hande, Güneş, · 2015 · New Trends in Mathematical Science
Full title: Oksijen aktarım koşullarının pichia pastoris ile gliseraldehit-3-fosfat dehidrojenaz promotörü altında rekombinant glukoz izomeraz üretimine etkisi.
Authors: Hande, Güneş,
Year: 2015
Venue: New Trends in Mathematical Science
PaperAn effective and simpli fi ed DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris
L. D. Picotto, G. Sguazza, M. A. Tizzano, C. Galosi, S. Cavalitto, M. Pecoraro
Full title: An effective and simpli fi ed DO-stat control strategy for production of rabies glycoprotein in Pichia pastoris
Authors: L. D. Picotto, G. Sguazza, M. A. Tizzano, C. Galosi, S. Cavalitto, M. Pecoraro
PaperHGM-CSF ( human granulocyte-macrophcolony stimulating factor ) tái tổ hợp trên hệ thống Pichia pastoris
Phạm Thị Tố Liên
Full title: HGM-CSF ( human granulocyte-macrophcolony stimulating factor ) tái tổ hợp trên hệ thống Pichia pastoris
Authors: Phạm Thị Tố Liên
PaperOptimisation of the production of cathepsin L1 from a recombinant saccharomyces cerevisiae
O’Donovan, Eimear · 2002 · Dublin City University Open Access Institutional Repository (Dublin City University)
Full title: Optimisation of the production of cathepsin L1 from a recombinant saccharomyces cerevisiae
Authors: O’Donovan, Eimear
Year: 2002
Venue: Dublin City University Open Access Institutional Repository (Dublin City University)
PaperOptimizing production methods for artificial silk proteins through bioreactor and purification studies of recombinant proteins expressed from Pichia pastoris
Ramey, Aaron Thomson · 2006
Full title: Optimizing production methods for artificial silk proteins through bioreactor and purification studies of recombinant proteins expressed from Pichia pastoris
Authors: Ramey, Aaron Thomson
Year: 2006
Paper[Overexpression of Escherchia coli phytase with high specific activity].
Luo, Hui-Ying, Yao, Bin, Yuan, Tie-Zheng, Wang, Ya-Rul, Shi, Xiu-Yun, Wu, Ning-Feng, Fan, Yun-Liu · 2004 · Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Full title: [Overexpression of Escherchia coli phytase with high specific activity].
Venue: Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Paper[Overexpression of Penicillium expansum lipase gene in Pichia pastoris].
Cai Yuan, Lin Lin, Qiao-qin Shi, Song-gang Wu · 2003 · Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Full title: [Overexpression of Penicillium expansum lipase gene in Pichia pastoris].
Authors: Cai Yuan, Lin Lin, Qiao-qin Shi, Song-gang Wu
Year: 2003
Venue: Sheng wu gong cheng xue bao = Chinese journal of biotechnology
PaperPeriplasmic expression of D1.3 Fab antibody in E. coli
Arasteh, Shaghayegh · 2012 · University of Birmingham Institutional Research Archive (University of Birmingham)
Full title: Periplasmic expression of D1.3 Fab antibody in E. coli
Authors: Arasteh, Shaghayegh
Year: 2012
Venue: University of Birmingham Institutional Research Archive (University of Birmingham)
PaperPichia pastoris ile insan büyüme hormonu üretimi için belenme stratejilerinin geliştirilmesi.
Bahar, Bozkurt, · 2012 · New Trends in Mathematical Science
Full title: Pichia pastoris ile insan büyüme hormonu üretimi için belenme stratejilerinin geliştirilmesi.
Authors: Bahar, Bozkurt,
Year: 2012
Venue: New Trends in Mathematical Science
PaperPichia pastoris ile PADH2-Cat8-L2 promotor altında rekombinant insan büyüme hormonu üretimi için etanol-temelli biyoreaktör işletim stratejisi geliştirilmesi ve modellenmesi.
Omar, Wehbe Al Masri, · 2019 · New Trends in Mathematical Science
Full title: Pichia pastoris ile PADH2-Cat8-L2 promotor altında rekombinant insan büyüme hormonu üretimi için etanol-temelli biyoreaktör işletim stratejisi geliştirilmesi ve modellenmesi.
Authors: Omar, Wehbe Al Masri,
Year: 2019
Venue: New Trends in Mathematical Science
PaperPichia pastoris ile rekombinant insan eritropoietin üretiminde ikinci karbon kaynaklarının etkileri.
Melda, Eskitoros, Şükran · 2013 · New Trends in Mathematical Science
Full title: Pichia pastoris ile rekombinant insan eritropoietin üretiminde ikinci karbon kaynaklarının etkileri.
Authors: Melda, Eskitoros, Şükran
Year: 2013
Venue: New Trends in Mathematical Science
PaperProduction of carcinoembryonic antigen (CEA) N-A3 domain in Pichia pastoris by fermentation.
Hellwig, S, Robin, F, Drossard, J, Raven, N P, Vaquero-Martin, C, Shively, J E, Fischer, R · 1999 · Biotechnology and applied biochemistry
Full title: Production of carcinoembryonic antigen (CEA) N-A3 domain in Pichia pastoris by fermentation.
Authors: Hellwig, S, Robin, F, Drossard, J, Raven, N P, Vaquero-Martin, C, Shively, J E, Fischer, R
Year: 1999
Venue: Biotechnology and applied biochemistry
PaperProduction of Recombinant Lectins from Garlic (Allium Sativum) Bulbs and Their Insecticidal Activity Against Hemipteran Insects.
Wiles, Duncan Peter · 2008 · Durham e-Theses (Durham University)
Full title: Production of Recombinant Lectins from Garlic (Allium Sativum) Bulbs and Their Insecticidal Activity Against Hemipteran Insects.
Authors: Wiles, Duncan Peter
Year: 2008
Venue: Durham e-Theses (Durham University)
PaperProzessnahe Hochdurchsatzoptimierung der heterologen Proteinproduktion in alternativen Wirtsorganismen
Kurt-Gür, Günseli · 2011 · Istanbul Technical University Academic Open Archive (Istanbul Technical University)
Full title: Pycnoporus Sanguıneus’tan Lakkaz Cdna’larının Klonlanması, Pıchıa Pastorıs’te Ekspresyonu Ve Rekombinant Lakkazların Karakterizasyonu
Authors: Kurt-Gür, Günseli
Year: 2011
Venue: Istanbul Technical University Academic Open Archive (Istanbul Technical University)
PaperRekombinant hücre içi glukoz izomeraz üretimi için escherichia coli ve pichia pastoris üretim potansiyellerinin kıyaslanması.
Sena, Yaman, · 2014 · New Trends in Mathematical Science
Full title: Rekombinant hücre içi glukoz izomeraz üretimi için escherichia coli ve pichia pastoris üretim potansiyellerinin kıyaslanması.
Authors: Sena, Yaman,
Year: 2014
Venue: New Trends in Mathematical Science
PaperRevisión de tema: pichia pastoris, una alternativa para la producción de glicoproteínas humanas de uso terapéutico. Estrategias de fermentación
Henry Córdoba Ruiz, Néstor Algecira Encizo, Raúl Alberto Poutou Piñales, L. Avellaneda · 2003
Full title: Revisión de tema: pichia pastoris, una alternativa para la producción de glicoproteínas humanas de uso terapéutico. Estrategias de fermentación
Authors: Henry Córdoba Ruiz, Néstor Algecira Encizo, Raúl Alberto Poutou Piñales, L. Avellaneda
Year: 2003
Paper[Secretory expression of recombinant porcine zona pellucida glycoprotein-3alpha (rpZP3alpha) in Pichia pastoris].
Gao, Yan-Ru, Pan, Shan-Pei, Xie, Qi-Xuan, Xiao, Luan-Juan, Ke, Qiong, Li, Wen-Xing, Shi, Wei-Wei · 2006 · Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Full title: [Secretory expression of recombinant porcine zona pellucida glycoprotein-3alpha (rpZP3alpha) in Pichia pastoris].
Venue: Sheng wu gong cheng xue bao = Chinese journal of biotechnology
PaperSPECTROSCOPIC STUDIES OF NOVEL MICROBIAL RHODOPSINS FROM FUNGI AND BACTERIA
Fan, Ying · 2011
Full title: SPECTROSCOPIC STUDIES OF NOVEL MICROBIAL RHODOPSINS FROM FUNGI AND BACTERIA
Authors: Fan, Ying
Year: 2011
PaperSpoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially therapeutic recombinant glycoprotein factor H
Devlin, John P. · 2018 · Edinburgh Research Archive (University of Edinburgh)
Full title: Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially therapeutic recombinant glycoprotein factor H
Authors: Devlin, John P.
Year: 2018
Venue: Edinburgh Research Archive (University of Edinburgh)
PaperStrategies for functionalization of recombinant spider silk
Jansson, Ronnie · 2015 · Epsilon Open Archive (Sveriges lantbruksuniversitet biblioteket (Swedish University of Agricultural Sciences))
Full title: Strategies for functionalization of recombinant spider silk
Authors: Jansson, Ronnie
Year: 2015
Venue: Epsilon Open Archive (Sveriges lantbruksuniversitet biblioteket (Swedish University of Agricultural Sciences))
PaperSTRATEGY DESIGN FOR THERAPEUTICAL PROTEIN PRODUCTION by Pichia pastoris
P. Çalık · 2013
Full title: STRATEGY DESIGN FOR THERAPEUTICAL PROTEIN PRODUCTION by Pichia pastoris
Authors: P. Çalık
Year: 2013
PaperStudien zur Optimierung der rekombinanten Genexpression in der methylotrophen Hefe Pichia pastoris
Kabaoglu, Fatma · 2005 · KOPS (University of Konstanz)
Full title: Studien zur Optimierung der rekombinanten Genexpression in der methylotrophen Hefe Pichia pastoris
Authors: Kabaoglu, Fatma
Year: 2005
Venue: KOPS (University of Konstanz)
Paper[Studies on fermentation conditions and purification of mutant human interleukin-2 expressed in Pichia pastoris].
Venue: Sheng wu gong cheng xue bao = Chinese journal of biotechnology
PaperTransgenic Plant and Fungal Expression to Assay in vitro and in planta Activity of Sus scrofa beta-Defensin 1 and Nicotiana tabacum Defensin 1
Atnaseo, C. · 2011
Full title: Transgenic Plant and Fungal Expression to Assay in vitro and in planta Activity of Sus scrofa beta-Defensin 1 and Nicotiana tabacum Defensin 1
Authors: Atnaseo, C.
Year: 2011
PaperÉtude de l'influence du méthanol sur la production extracellulaire de la GFP par Pichia pastoris.
C. Desautels · 2001
Full title: Étude de l'influence du méthanol sur la production extracellulaire de la GFP par Pichia pastoris.
Authors: C. Desautels
Year: 2001
PaperTwo-hybrid analysis and attempted expression of elongation factor 1α from the cattle tick, Rhipicephalus microplus.
Full title: Two-hybrid analysis and attempted expression of elongation factor 1α from the cattle tick, Rhipicephalus microplus.
Authors: Botha, Melissa
Year: 2013
Venue: UpSpace Institutional Repository (University of Pretoria)
PaperUnderstanding and Engineering Ester Biosynthesis Pathways in the Yeast Kluyveromyces marxianus
Loebs, Ann-Kathrin · 2018 · eScholarship (California Digital Library)
Full title: Understanding and Engineering Ester Biosynthesis Pathways in the Yeast Kluyveromyces marxianus
Authors: Loebs, Ann-Kathrin
Year: 2018
Venue: eScholarship (California Digital Library)
PaperUsing methanol / sorbitol mixtures as carbon source enables safer and cheaper recombinant protein production from Pichia Pastoris by reducing the need for pure methanol and oxygen feeding
Wang, B, Nesbeth, Darren, Keshavarz‐Moore, Eli · 2017 · UCL Discovery (University College London)
Full title: Using methanol / sorbitol mixtures as carbon source enables safer and cheaper recombinant protein production from Pichia Pastoris by reducing the need for pure methanol and oxygen feeding
Authors: Wang, B, Nesbeth, Darren, Keshavarz‐Moore, Eli
Year: 2017
Venue: UCL Discovery (University College London)
PaperCharacterization of Trichoderma reesei cellobiohydrolase Cel7A secreted from Pichia pastoris using two different promoters.
Boer, H, Teeri, T T, Koivula, A · 2000 · Biotechnology and bioengineering · DOI
Full title: Characterization of Trichoderma reesei cellobiohydrolase Cel7A secreted from Pichia pastoris using two different promoters.
PaperExpression and characterization of a cellobiohydrolase (CBH7B) from the thermophilic fungus Thielavia terrestris in Pichia pastoris.
Woon, James Sy-Keen, Mackeen, Mukram Mohamed, Mahadi, Nor Muhammad, Illias, Rosli Md, Abdul Murad, Abdul Munir, Abu Bakar, Farah Diba · 2016 · Biotechnology and applied biochemistry · DOI
Full title: Expression and characterization of a cellobiohydrolase (CBH7B) from the thermophilic fungus Thielavia terrestris in Pichia pastoris.
Authors: Woon, James Sy-Keen, Mackeen, Mukram Mohamed, Mahadi, Nor Muhammad, Illias, Rosli Md, Abdul Murad, Abdul Munir, Abu Bakar, Farah Diba
PaperRSM optimization of HSA/IL1Ra in <i>Pichia pastoris</i> overexpression strain and study of its <i>in vivo</i> activity in reducing hyperglycemia of GK rats
Full title: RSM optimization of HSA/IL1Ra in <i>Pichia pastoris</i> overexpression strain and study of its <i>in vivo</i> activity in reducing hyperglycemia of GK rats
PaperCo‐Expression of Chaperones for Improvement of Recombinant <i>Geobacillus stearothermophilus</i> Lipase Production in <i>Pichia pastoris</i>
Erden‐Karaoğlan, Fidan · 2025 · Biotechnology and Applied Biochemistry · DOI
Full title: Co‐Expression of Chaperones for Improvement of Recombinant <i>Geobacillus stearothermophilus</i> Lipase Production in <i>Pichia pastoris</i>
PaperComparison of the baculovirus-insect cell and Pichia pastoris heterologous systems for the expression of the human tumor suppressor protein RNASET2.
Campomenosi, Paola, Cinquetti, Raffaella, Tallarita, Elena, Lindqvist, Christer, Raimondi, Ivan, Grassi, Paola, Näsman, Johnny, Dell, Anne, Haslam, Stuart M, Taramelli, Roberto, Acquati, Francesco · 2011 · Biotechnology and applied biochemistry · DOI
Full title: Comparison of the baculovirus-insect cell and Pichia pastoris heterologous systems for the expression of the human tumor suppressor protein RNASET2.
Authors: Campomenosi, Paola, Cinquetti, Raffaella, Tallarita, Elena, Lindqvist, Christer, Raimondi, Ivan, Grassi, Paola, Näsman, Johnny, Dell, Anne, Haslam, Stuart M, Taramelli, Roberto, Acquati, Francesco
PaperGenome‐scale metabolic model of methylotrophic yeast <i>Pichia pastoris</i> and its use for <i>in silico</i> analysis of heterologous protein production
Sohn, Seung Bum, Graf, Alexandra B., Kim, Tae Yong, Gasser, Brigitte, Maurer, Michael, Ferrer, Pau, Mattanovich, Diethard, Lee, Sang Yup · 2010 · Biotechnology Journal · DOI
Full title: Genome‐scale metabolic model of methylotrophic yeast <i>Pichia pastoris</i> and its use for <i>in silico</i> analysis of heterologous protein production
Authors: Sohn, Seung Bum, Graf, Alexandra B., Kim, Tae Yong, Gasser, Brigitte, Maurer, Michael, Ferrer, Pau, Mattanovich, Diethard, Lee, Sang Yup
Paper<i>Pichia pastoris</i> secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space‐time yields for less complex proteins
Full title: <i>Pichia pastoris</i> secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space‐time yields for less complex proteins
PaperFunctionalized silk assembled from a recombinant spider silk fusion protein (Z-4RepCT) produced in the methylotrophic yeast Pichia pastoris.
Jansson, Ronnie, Lau, Cheuk H, Ishida, Takuya, Ramström, Margareta, Sandgren, Mats, Hedhammar, My · 2016 · Biotechnology journal · DOI
Full title: Functionalized silk assembled from a recombinant spider silk fusion protein (Z-4RepCT) produced in the methylotrophic yeast Pichia pastoris.
PaperMicroscale Perfusion‐Based Cultivation for <i>Pichia pastoris</i> Clone Screening Enables Accelerated and Optimized Recombinant Protein Production Processes
Full title: Microscale Perfusion‐Based Cultivation for <i>Pichia pastoris</i> Clone Screening Enables Accelerated and Optimized Recombinant Protein Production Processes
PaperMethanol induction optimization for scFv antibody fragment production in Pichia pastoris.
Cunha, A E, Clemente, J J, Gomes, R, Pinto, F, Thomaz, M, Miranda, S, Pinto, R, Moosmayer, D, Donner, P, Carrondo, M J T · 2004 · Biotechnology and bioengineering · DOI
Full title: Methanol induction optimization for scFv antibody fragment production in Pichia pastoris.
Authors: Cunha, A E, Clemente, J J, Gomes, R, Pinto, F, Thomaz, M, Miranda, S, Pinto, R, Moosmayer, D, Donner, P, Carrondo, M J T
PaperInfluence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut+ strain.
Schenk, Jonas, Balazs, Krisztina, Jungo, Carmen, Urfer, Julien, Wegmann, Carole, Zocchi, Andrea, Marison, Ian W, von Stockar, Urs · 2008 · Biotechnology and bioengineering · DOI
Full title: Influence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut+ strain.
Authors: Schenk, Jonas, Balazs, Krisztina, Jungo, Carmen, Urfer, Julien, Wegmann, Carole, Zocchi, Andrea, Marison, Ian W, von Stockar, Urs
PaperPolymer characterization and optimization of conditions for the enhanced bioproduction of benzaldehyde by <i>Pichia pastoris</i> in a two‐ ;phase partitioning bioreactor
Craig, Tom, Daugulis, Andrew J. · 2012 · Biotechnology and Bioengineering · DOI
Full title: Polymer characterization and optimization of conditions for the enhanced bioproduction of benzaldehyde by <i>Pichia pastoris</i> in a two‐ ;phase partitioning bioreactor
PaperComparative thermal inactivation analysis of Aspergillus oryzae and Thiellavia terrestris cutinase: Role of glycosylation.
Shirke, Abhijit N, Su, An, Jones, J Andrew, Butterfoss, Glenn L, Koffas, Mattheos A G, Kim, Jin Ryoun, Gross, Richard A · 2017 · Biotechnology and bioengineering · DOI
Full title: Comparative thermal inactivation analysis of Aspergillus oryzae and Thiellavia terrestris cutinase: Role of glycosylation.
Authors: Shirke, Abhijit N, Su, An, Jones, J Andrew, Butterfoss, Glenn L, Koffas, Mattheos A G, Kim, Jin Ryoun, Gross, Richard A
PaperMetabolic modeling to identify engineering targets for <i>Komagataella phaffii</i>: The effect of biomass composition on gene target identification
Cankorur‐Cetinkaya, Ayca, Dikicioǧlu, Duygu, Oliver, Stephen G. · 2017 · Biotechnology and Bioengineering · DOI
Full title: Metabolic modeling to identify engineering targets for <i>Komagataella phaffii</i>: The effect of biomass composition on gene target identification
Authors: Cankorur‐Cetinkaya, Ayca, Dikicioǧlu, Duygu, Oliver, Stephen G.
PaperSuperior protein titers in half the fermentation time: Promoter and process engineering for the glucose‐regulated <i>GTH1</i> promoter of <i>Pichia pastoris</i>
Full title: Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose‐regulated <i>GTH1</i> promoter of <i>Pichia pastoris</i>
PaperProcess development for the continuous production of heterologous proteins by the industrial yeast, <i>Komagataella phaffii</i>
Cankorur‐Cetinkaya, Ayca, Narraidoo, Nathalie, Kasavi, Ceyda, Slater, Nigel K.H., Archer, David B., Oliver, Stephen G. · 2018 · Biotechnology and Bioengineering · DOI
Full title: Process development for the continuous production of heterologous proteins by the industrial yeast, <i>Komagataella phaffii</i>
Authors: Cankorur‐Cetinkaya, Ayca, Narraidoo, Nathalie, Kasavi, Ceyda, Slater, Nigel K.H., Archer, David B., Oliver, Stephen G.
PaperChallenging the workhorse: Comparative analysis of eukaryotic micro‐organisms for expressing monoclonal antibodies
Jiang, Hanxiao, Horwitz, Andrew A., Wright, Chapman, Tai, Anna, Znameroski, Elizabeth A., Tsegaye, Yoseph, Warbington, Hailley, Bower, Benjamin S., Alves, Christina, Co, Carl, Jonnalagadda, Kanvasri N., Platt, Darren, Walter, Jessica M., Natarajan, Venkatesh, Ubersax, Jeffrey A., Cherry, Joel R., Love, J. Christopher · 2019 · Biotechnology and Bioengineering · DOI
Full title: Challenging the workhorse: Comparative analysis of eukaryotic micro‐organisms for expressing monoclonal antibodies
Authors: Jiang, Hanxiao, Horwitz, Andrew A., Wright, Chapman, Tai, Anna, Znameroski, Elizabeth A., Tsegaye, Yoseph, Warbington, Hailley, Bower, Benjamin S., Alves, Christina, Co, Carl, Jonnalagadda, Kanvasri N., Platt, Darren, Walter, Jessica M., Natarajan, Venkatesh, Ubersax, Jeffrey A., Cherry, Joel R., Love, J. Christopher
PaperPhysiological responses of <i>Saccharomyces cerevisiae</i> to industrially relevant conditions: Slow growth, low pH, and high CO<sub>2</sub> levels
Hakkaart, Xavier, Liu, Yaya, Hulst, Mandy B., Masoudi, Anissa el, Peuscher, Eveline, Pronk, Jack T., Gulik, Walter van, Daran‐Lapujade, Pascale · 2019 · Biotechnology and Bioengineering · DOI
Full title: Physiological responses of <i>Saccharomyces cerevisiae</i> to industrially relevant conditions: Slow growth, low pH, and high CO<sub>2</sub> levels
Authors: Hakkaart, Xavier, Liu, Yaya, Hulst, Mandy B., Masoudi, Anissa el, Peuscher, Eveline, Pronk, Jack T., Gulik, Walter van, Daran‐Lapujade, Pascale
PaperCharacterization of methanol utilization negative <i>Pichia pastoris</i> for secreted protein production: New cultivation strategies for current and future applications
Zavec, Domen, Gasser, Brigitte, Mattanovich, Diethard · 2020 · Biotechnology and Bioengineering · DOI
Full title: Characterization of methanol utilization negative <i>Pichia pastoris</i> for secreted protein production: New cultivation strategies for current and future applications
PaperModular development enables rapid design of media for alternative hosts
Biedermann, Andrew M., Gengaro, Isabella R., Rodriguez‐Aponte, Sergio A., Love, Kerry R., Love, J. Christopher · 2021 · Biotechnology and Bioengineering · DOI
Full title: Modular development enables rapid design of media for alternative hosts
Authors: Biedermann, Andrew M., Gengaro, Isabella R., Rodriguez‐Aponte, Sergio A., Love, Kerry R., Love, J. Christopher
PaperCloning and expression of a functional core streptavidin in <i>Pichia pastoris:</i> Strategies to increase yield
Casteluber, Marisa C. F., Damasceno, Leonardo M., Silveira, Wendel Batista da, Diniz, Raphael Hermano Santos, Passos, Frederico José Vieira, Passos, Flávia Maria Lopes, Passos, Flávia M. L., Passos, Flávia M. L. · 2012 · Biotechnology Progress · DOI
Full title: Cloning and expression of a functional core streptavidin in <i>Pichia pastoris:</i> Strategies to increase yield
Authors: Casteluber, Marisa C. F., Damasceno, Leonardo M., Silveira, Wendel Batista da, Diniz, Raphael Hermano Santos, Passos, Frederico José Vieira, Passos, Flávia Maria Lopes, Passos, Flávia M. L., Passos, Flávia M. L.
PaperOptimization of five environmental factors to increase beta‐propeller phytase production in Pichia pastoris and impact on the physiological response of the host
J. M. Viader-Salvadó, M. Castillo-Galván, J. A. Fuentes-Garibay, M. Iracheta-Cárdenas, M. Guerrero-Olazarán · 2013 · Biotechnology progress (Print) · DOI
Full title: Optimization of five environmental factors to increase beta‐propeller phytase production in Pichia pastoris and impact on the physiological response of the host
Authors: J. M. Viader-Salvadó, M. Castillo-Galván, J. A. Fuentes-Garibay, M. Iracheta-Cárdenas, M. Guerrero-Olazarán
PaperProduction of a sterol esterase from Ophiostoma piceae in batch and fed‐batch bioprocesses using different Pichia pastoris phenotypes as cell factory
Víctor Barba Cedillo, M. Martínez, C. Arnau, F. Valero · 2014 · Biotechnology progress (Print) · DOI
Full title: Production of a sterol esterase from Ophiostoma piceae in batch and fed‐batch bioprocesses using different Pichia pastoris phenotypes as cell factory
Authors: Víctor Barba Cedillo, M. Martínez, C. Arnau, F. Valero
PaperEffect of dilution rate and methanol-glycerol mixed feeding on heterologous Rhizopus oryzae lipase production with Pichia pastoris Mut(+) phenotype in continuous culture.
Canales, Christian, Altamirano, Claudia, Berrios, Julio · 2015 · Biotechnology progress · DOI
Full title: Effect of dilution rate and methanol-glycerol mixed feeding on heterologous Rhizopus oryzae lipase production with Pichia pastoris Mut(+) phenotype in continuous culture.
Authors: Canales, Christian, Altamirano, Claudia, Berrios, Julio
PaperOptimization of norovirus virus‐like particle production in <i>Pichia pastoris</i> using a real‐time near‐infrared bioprocess monitor
Parker, S., Maloy, Mitchell H., Tomé‐Amat, Jaime, Bardliving, Cameron, Batt, Carl A., Lanz, Kaylee J., Olesberg, J. T., Arnold, Mark A. · 2016 · Biotechnology Progress · DOI
Full title: Optimization of norovirus virus‐like particle production in <i>Pichia pastoris</i> using a real‐time near‐infrared bioprocess monitor
Authors: Parker, S., Maloy, Mitchell H., Tomé‐Amat, Jaime, Bardliving, Cameron, Batt, Carl A., Lanz, Kaylee J., Olesberg, J. T., Arnold, Mark A.
PaperRecombinant exochitinase of the thermophilic mould Myceliopthora thermophila BJA: Characteristics and utility in generating N-acetyl glucosamine and in biocontrol of phytopathogenic fungi.
Dua, Ashima, Joshi, Swati, Satyanarayana, T · 2017 · Biotechnology progress · DOI
Full title: Recombinant exochitinase of the thermophilic mould Myceliopthora thermophila BJA: Characteristics and utility in generating N-acetyl glucosamine and in biocontrol of phytopathogenic fungi.
Authors: Dua, Ashima, Joshi, Swati, Satyanarayana, T
PaperBiotransformation of β‐hydroxypyruvate and glycolaldehyde to l ‐erythrulose by <i>Pichia pastoris</i> strain GS115 overexpressing native transketolase
Wei, Yu‐Chia, Braun‐Galleani, Stephanie, Henríquez, María José, Bandara, Sahan, Nesbeth, Darren · 2017 · Biotechnology Progress · DOI
Full title: Biotransformation of β‐hydroxypyruvate and glycolaldehyde to l ‐erythrulose by <i>Pichia pastoris</i> strain GS115 overexpressing native transketolase
Authors: Wei, Yu‐Chia, Braun‐Galleani, Stephanie, Henríquez, María José, Bandara, Sahan, Nesbeth, Darren
PaperHeterologous expression, purification, and functional characterization of recombinant ovine angiotensinogen in the methylotrophic yeast Pichia pastoris.
Full title: Heterologous expression, purification, and functional characterization of recombinant ovine angiotensinogen in the methylotrophic yeast Pichia pastoris.
PaperHolistic process development to mitigate proteolysis of a subunit rotavirus vaccine candidate produced in <i>Pichia pastoris</i> by means of an acid pH pulse during fed‐batch fermentation
Velez‐Suberbie, M. Lourdes, Morris, Stephen, Kaur, Kawaljit, Hickey, John M., Joshi, Sangeeta B., Volkin, David B., Bracewell, Daniel G., Mukhopadhyay, Tarit · 2020 · Biotechnology Progress · DOI
Full title: Holistic process development to mitigate proteolysis of a subunit rotavirus vaccine candidate produced in <i>Pichia pastoris</i> by means of an acid pH pulse during fed‐batch fermentation
Authors: Velez‐Suberbie, M. Lourdes, Morris, Stephen, Kaur, Kawaljit, Hickey, John M., Joshi, Sangeeta B., Volkin, David B., Bracewell, Daniel G., Mukhopadhyay, Tarit
PaperEffect of Glycosylation on the Biocatalytic Properties of Hydroxynitrile Lyase from the Passion Fruit, Passiflora edulis: A Comparison of Natural and Recombinant Enzymes.
Nuylert, Aem, Ishida, Yuko, Asano, Yasuhisa · 2017 · Chembiochem : a European journal of chemical biology · DOI
Full title: Effect of Glycosylation on the Biocatalytic Properties of Hydroxynitrile Lyase from the Passion Fruit, Passiflora edulis: A Comparison of Natural and Recombinant Enzymes.
PaperComparing the Catalytic and Structural Characteristics of a ‘Short’ Unspecific Peroxygenase (UPO) Expressed in <i>Pichia pastoris</i> and <i>Escherichia coli</i>
Full title: Comparing the Catalytic and Structural Characteristics of a ‘Short’ Unspecific Peroxygenase (UPO) Expressed in <i>Pichia pastoris</i> and <i>Escherichia coli</i>
PaperAdvanced monitoring and control of pharmaceutical production processes with <i>Pichia pastoris</i> by using Raman spectroscopy and multivariate calibration methods
Voss, Jan‐Patrick, Mittelheuser, Nina E., Lemke, Roman, Luttmann, R. · 2017 · Engineering in Life Sciences · DOI
Full title: Advanced monitoring and control of pharmaceutical production processes with <i>Pichia pastoris</i> by using Raman spectroscopy and multivariate calibration methods
Authors: Voss, Jan‐Patrick, Mittelheuser, Nina E., Lemke, Roman, Luttmann, R.
PaperCo‐substrate mannitol feeding strategy design in semi‐batch production of recombinant human erythropoietin production by <i>Pichia pastoris</i>
Eskitoros, Melda Ş., Çalı́k, Pınar · 2013 · Journal of Chemical Technology & Biotechnology · DOI
Full title: Co‐substrate mannitol feeding strategy design in semi‐batch production of recombinant human erythropoietin production by <i>Pichia pastoris</i>
Authors: Eskitoros, Melda Ş., Çalı́k, Pınar
Year: 2013
Venue: Journal of Chemical Technology & Biotechnology
PaperTowards optimal substrate feeding for heterologous protein production in <i>Pichia pastoris (Komagataella spp)</i> fed‐batch processes under P<sub><i>AOX1</i></sub> control: a modeling aided approach
Ponte, Xavier, Barrigón, José Manuel, Maurer, Michael, Mattanovich, Diethard, Valero, Francisco, Montesinos, José Luis González · 2018 · Journal of Chemical Technology & Biotechnology · DOI
Full title: Towards optimal substrate feeding for heterologous protein production in <i>Pichia pastoris (Komagataella spp)</i> fed‐batch processes under P<sub><i>AOX1</i></sub> control: a modeling aided approach
Authors: Ponte, Xavier, Barrigón, José Manuel, Maurer, Michael, Mattanovich, Diethard, Valero, Francisco, Montesinos, José Luis González
Year: 2018
Venue: Journal of Chemical Technology & Biotechnology
PaperComparative study of <i>μ</i>‐stat methanol feeding control in fed‐batch fermentation of <i>Pichia pastoris</i> producing HBsAg: an open‐loop control <i>versus</i> recurrent artificial neural network‐based feedback control
Beiroti, Ahmad, Hosseini, Seyed Nezamedin, Aghasadeghi, Mohammad Reza, Norouzian, Dariush · 2019 · Journal of Chemical Technology & Biotechnology · DOI
Full title: Comparative study of <i>μ</i>‐stat methanol feeding control in fed‐batch fermentation of <i>Pichia pastoris</i> producing HBsAg: an open‐loop control <i>versus</i> recurrent artificial neural network‐based feedback control
PaperPhysical stability comparisons of IgG1-Fc variants: effects of N-glycosylation site occupancy and Asp/Gln residues at site Asn 297.
Alsenaidy, Mohammad A, Okbazghi, Solomon Z, Kim, Jae Hyun, Joshi, Sangeeta B, Middaugh, C Russell, Tolbert, Thomas J, Volkin, David B · 2014 · Journal of pharmaceutical sciences · DOI
Full title: Physical stability comparisons of IgG1-Fc variants: effects of N-glycosylation site occupancy and Asp/Gln residues at site Asn 297.
Authors: Alsenaidy, Mohammad A, Okbazghi, Solomon Z, Kim, Jae Hyun, Joshi, Sangeeta B, Middaugh, C Russell, Tolbert, Thomas J, Volkin, David B
PaperEfficient expression, purification and characterization of mouse salivary alpha-amylase secreted from methylotrophic yeast, Pichia pastoris.
Kato, S, Ishibashi, M, Tatsuda, D, Tokunaga, H, Tokunaga, M · 2001 · Yeast (Chichester, England) · DOI
Full title: Efficient expression, purification and characterization of mouse salivary alpha-amylase secreted from methylotrophic yeast, Pichia pastoris.
Authors: Kato, S, Ishibashi, M, Tatsuda, D, Tokunaga, H, Tokunaga, M
PaperPost-translational modifications of the AFET3 gene product: a component of the iron transport system in budding cells and mycelia of the yeast Arxula adeninivorans.
Full title: Post-translational modifications of the AFET3 gene product: a component of the iron transport system in budding cells and mycelia of the yeast Arxula adeninivorans.
PaperSite-directed mutagenesis improves catalytic efficiency and thermostability of Escherichia coli pH 2.5 acid phosphatase/phytase expressed in Pichia pastoris.
Rodriguez, E, Wood, Z A, Karplus, P A, Lei, X G · 2000 · Archives of biochemistry and biophysics · DOI
Full title: Site-directed mutagenesis improves catalytic efficiency and thermostability of Escherichia coli pH 2.5 acid phosphatase/phytase expressed in Pichia pastoris.
Authors: Rodriguez, E, Wood, Z A, Karplus, P A, Lei, X G
PaperExpression and characterization of glycosylated and catalytically active recombinant human alpha-galactosidase A produced in Pichia pastoris.
Chen, Y, Jin, M, Egborge, T, Coppola, G, Andre, J, Calhoun, D H · 2000 · Protein expression and purification · DOI
Full title: Expression and characterization of glycosylated and catalytically active recombinant human alpha-galactosidase A produced in Pichia pastoris.
Authors: Chen, Y, Jin, M, Egborge, T, Coppola, G, Andre, J, Calhoun, D H
PaperExtensive N-glycosylation reduces the thermal stability of a recombinant alkalophilic bacillus alpha-amylase produced in Pichia pastoris.
Tull, D, Gottschalk, T E, Svendsen, I, Kramhøft, B, Phillipson, B A, Bisgård-Frantzen, H, Olsen, O, Svensson, B · 2001 · Protein expression and purification · DOI
Full title: Extensive N-glycosylation reduces the thermal stability of a recombinant alkalophilic bacillus alpha-amylase produced in Pichia pastoris.
Authors: Tull, D, Gottschalk, T E, Svendsen, I, Kramhøft, B, Phillipson, B A, Bisgård-Frantzen, H, Olsen, O, Svensson, B
PaperDissolved-oxygen-stat controlling two variables for methanol induction of rGuamerin in Pichia pastoris and its application to repeated fed-batch
H.-K. Lim, S. Choi, K. Kim, K. Jung · 2003 · Applied Microbiology and Biotechnology · DOI
Full title: Dissolved-oxygen-stat controlling two variables for methanol induction of rGuamerin in Pichia pastoris and its application to repeated fed-batch
PaperProcess optimization of constitutive human granulocyte-macrophage colony-stimulating factor (hGM-CSF) expression in Pichia pastoris fed-batch culture.
Pal, Yogender, Khushoo, Amardeep, Mukherjee, K J · 2006 · Applied microbiology and biotechnology · DOI
Full title: Process optimization of constitutive human granulocyte-macrophage colony-stimulating factor (hGM-CSF) expression in Pichia pastoris fed-batch culture.
Authors: Pal, Yogender, Khushoo, Amardeep, Mukherjee, K J
PaperHigh-level expression of a truncated 1,3-1,4-β-d-glucanase from Fibrobacter succinogenes in Pichia pastoris by optimization of codons and fermentation
Huo-qing Huang, Peilong Yang, Huiying Luo, Huashan Tang, Na Shao, T. Yuan, Ya-ru Wang, Yingguo Bai, B. Yao · 2008 · Applied Microbiology and Biotechnology · DOI
Full title: High-level expression of a truncated 1,3-1,4-β-d-glucanase from Fibrobacter succinogenes in Pichia pastoris by optimization of codons and fermentation
Authors: Huo-qing Huang, Peilong Yang, Huiying Luo, Huashan Tang, Na Shao, T. Yuan, Ya-ru Wang, Yingguo Bai, B. Yao
PaperThermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: high-level expression in Pichia pastoris and characterization of the recombinant protein.
Berezina, Oksana V, Herlet, Jonathan, Rykov, Sergey V, Kornberger, Petra, Zavyalov, Artem, Kozlov, Dmitriy, Sakhibgaraeva, Liliya, Krestyanova, Irina, Schwarz, Wolfgang H, Zverlov, Vladimir V, Liebl, Wolfgang, Yarotsky, Sergey V · 2017 · Applied microbiology and biotechnology · DOI
Full title: Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: high-level expression in Pichia pastoris and characterization of the recombinant protein.
Authors: Berezina, Oksana V, Herlet, Jonathan, Rykov, Sergey V, Kornberger, Petra, Zavyalov, Artem, Kozlov, Dmitriy, Sakhibgaraeva, Liliya, Krestyanova, Irina, Schwarz, Wolfgang H, Zverlov, Vladimir V, Liebl, Wolfgang, Yarotsky, Sergey V
PaperA highly versatile fungal glucosyltransferase for specific production of quercetin-7-O-β-D-glucoside and quercetin-3-O-β-D-glucoside in different hosts.
Ren, Jie, Tang, Wenzhu, Barton, Caleb Don, Price, Owen M, Mortensen, Mark Wayne, Phillips, Alexandra, Wald, Banner, Hulme, Simon Elgin, Stanley, Logan Powell, Hevel, Joan, Zhan, Jixun · 2022 · Applied microbiology and biotechnology · DOI
Full title: A highly versatile fungal glucosyltransferase for specific production of quercetin-7-O-β-D-glucoside and quercetin-3-O-β-D-glucoside in different hosts.
Authors: Ren, Jie, Tang, Wenzhu, Barton, Caleb Don, Price, Owen M, Mortensen, Mark Wayne, Phillips, Alexandra, Wald, Banner, Hulme, Simon Elgin, Stanley, Logan Powell, Hevel, Joan, Zhan, Jixun
PaperMethanol-inducible promoter of thermotolerant methylotrophic yeast Ogataea thermomethanolica BCC16875 potential for production of heterologous protein at high temperatures.
Promdonkoy, Peerada, Tirasophon, Witoon, Roongsawang, Niran, Eurwilaichitr, Lily, Tanapongpipat, Sutipa · 2014 · Current microbiology · DOI
Full title: Methanol-inducible promoter of thermotolerant methylotrophic yeast Ogataea thermomethanolica BCC16875 potential for production of heterologous protein at high temperatures.
PaperProduction and purification of recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) from high cell density cultures of Pichia pastoris.
Bhatacharya, Palash, Pandey, Gaurav, Mukherjee, Krishna Jyoti · 2007 · Bioprocess and biosystems engineering · DOI
Full title: Production and purification of recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) from high cell density cultures of Pichia pastoris.
PaperCodon optimization of xylA gene for recombinant glucose isomerase production in Pichia pastoris and fed-batch feeding strategies to fine-tune bioreactor performance.
Ata, Özge, Boy, Erdem, Güneş, Hande, Çalık, Pınar · 2015 · Bioprocess and biosystems engineering · DOI
Full title: Codon optimization of xylA gene for recombinant glucose isomerase production in Pichia pastoris and fed-batch feeding strategies to fine-tune bioreactor performance.
PaperDevelopment of a dual specific growth rate-based fed-batch process for production of recombinant human granulocyte colony-stimulating factor in Pichia pastoris.
Gautam, Ashwani, Sahai, Vikram, Mishra, Saroj · 2021 · Bioprocess and biosystems engineering · DOI
Full title: Development of a dual specific growth rate-based fed-batch process for production of recombinant human granulocyte colony-stimulating factor in Pichia pastoris.
PaperHigh-level production of recombinant HBcAg virus-like particles in a mathematically modelled P. pastoris GS115 Mut+ bioreactor process under controlled residual methanol concentration
Bolmanis, Emīls, Grīgs, Oskars, Kazāks, Andris, Galvanauskas, Vytautas · 2022 · Bioprocess and Biosystems Engineering · DOI
Full title: High-level production of recombinant HBcAg virus-like particles in a mathematically modelled P. pastoris GS115 Mut+ bioreactor process under controlled residual methanol concentration
Authors: Bolmanis, Emīls, Grīgs, Oskars, Kazāks, Andris, Galvanauskas, Vytautas
PaperCharacterization of recombinant barley oxalate oxidase expressed by Pichia pastoris.
Whittaker, Mei M, Whittaker, James W · 2002 · Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · DOI
Full title: Characterization of recombinant barley oxalate oxidase expressed by Pichia pastoris.
Authors: Whittaker, Mei M, Whittaker, James W
Year: 2002
Venue: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
PaperConstitutive expression of human angiostatin in Pichia pastoris by high-density cell culture
Zhang, A. L., Zhang, T. Y., Luo, J. X., Chen, Senjie, Guan, Wenjun, Fu, C. Y., Peng, Shiguo, Li, H. L. · 2006 · Journal of Industrial Microbiology & Biotechnology · DOI
Full title: Constitutive expression of human angiostatin in Pichia pastoris by high-density cell culture
Authors: Zhang, A. L., Zhang, T. Y., Luo, J. X., Chen, Senjie, Guan, Wenjun, Fu, C. Y., Peng, Shiguo, Li, H. L.
Year: 2006
Venue: Journal of Industrial Microbiology & Biotechnology
Full title: Cloning and bioinformatics analysis of an endoglucanase gene (Aucel12A) from Aspergillus usamii and its functional expression in Pichia pastoris.
PaperA simple Pichia pastoris fermentation and downstream processing strategy for making recombinant pandemic Swine Origin Influenza a virus Hemagglutinin protein.
Athmaram, T N, Singh, Anil Kumar, Saraswat, Shweta, Srivastava, Saurabh, Misra, Princi, Kameswara Rao, M, Gopalan, N, Rao, P V L · 2013 · Journal of industrial microbiology & biotechnology · DOI
Full title: A simple Pichia pastoris fermentation and downstream processing strategy for making recombinant pandemic Swine Origin Influenza a virus Hemagglutinin protein.
Authors: Athmaram, T N, Singh, Anil Kumar, Saraswat, Shweta, Srivastava, Saurabh, Misra, Princi, Kameswara Rao, M, Gopalan, N, Rao, P V L
Year: 2013
Venue: Journal of industrial microbiology & biotechnology
PaperDirected modification of the <i>Aspergillus usamii</i> β-mannanase to improve its substrate affinity by in silico design and site-directed mutagenesis
Full title: Directed modification of the <i>Aspergillus usamii</i> β-mannanase to improve its substrate affinity by in silico design and site-directed mutagenesis
PaperOptimising expression of the recombinant fusion protein biopesticide ω-hexatoxin-Hv1a/GNA in<i>Pichia pastoris:</i>sequence modifications and a simple method for the generation of multi-copy strains
Pyati, Prashant, Fitches, Elaine, Gatehouse, John A. · 2014 · Journal of Industrial Microbiology & Biotechnology · DOI
Full title: Optimising expression of the recombinant fusion protein biopesticide ω-hexatoxin-Hv1a/GNA in<i>Pichia pastoris:</i>sequence modifications and a simple method for the generation of multi-copy strains
Authors: Pyati, Prashant, Fitches, Elaine, Gatehouse, John A.
Year: 2014
Venue: Journal of Industrial Microbiology & Biotechnology
PaperCombined use of <i>GAP</i> and <i>AOX1</i> promoters and optimization of culture conditions to enhance expression of <i>Rhizomucor miehei</i> lipase
Full title: Combined use of <i>GAP</i> and <i>AOX1</i> promoters and optimization of culture conditions to enhance expression of <i>Rhizomucor miehei</i> lipase
PaperRapid screening of cellular stress responses in recombinant <i>Pichia pastoris</i> strains using metabolite profiling
Tredwell, Gregory D., Aw, Rochelle, Edwards-Jones, Bryn, Leak, David J., Bundy, Jacob G. · 2017 · Journal of Industrial Microbiology & Biotechnology · DOI
Full title: Rapid screening of cellular stress responses in recombinant <i>Pichia pastoris</i> strains using metabolite profiling
Authors: Tredwell, Gregory D., Aw, Rochelle, Edwards-Jones, Bryn, Leak, David J., Bundy, Jacob G.
Year: 2017
Venue: Journal of Industrial Microbiology & Biotechnology
PaperParallelized microscale fed-batch cultivation in online-monitored microtiter plates: implications of media composition and feed strategies for process design and performance
Morschett, Holger, Jansen, Roman, Neuendorf, Christian Simon, Moch, Matthias, Wiechert, Wolfgang, Oldiges, Marco · 2019 · Journal of Industrial Microbiology & Biotechnology · DOI
Full title: Parallelized microscale fed-batch cultivation in online-monitored microtiter plates: implications of media composition and feed strategies for process design and performance
Authors: Morschett, Holger, Jansen, Roman, Neuendorf, Christian Simon, Moch, Matthias, Wiechert, Wolfgang, Oldiges, Marco
Year: 2019
Venue: Journal of Industrial Microbiology & Biotechnology
PaperTowards smart biomanufacturing: a perspective on recent developments in industrial measurement and monitoring technologies for bio-based production processes
Gargalo, Carina L., Udugama, Isuru A., Pontius, Katrin, López, Pau Cabañeros, Nielsen, Rasmus Fjordbak, Hasanzadeh, Aliyeh, Mansouri, Seyed Soheil, Bayer, Christoph, Junicke, Helena, Gernaey, Krist V. · 2020 · Journal of Industrial Microbiology & Biotechnology · DOI
Full title: Towards smart biomanufacturing: a perspective on recent developments in industrial measurement and monitoring technologies for bio-based production processes
Authors: Gargalo, Carina L., Udugama, Isuru A., Pontius, Katrin, López, Pau Cabañeros, Nielsen, Rasmus Fjordbak, Hasanzadeh, Aliyeh, Mansouri, Seyed Soheil, Bayer, Christoph, Junicke, Helena, Gernaey, Krist V.
Year: 2020
Venue: Journal of Industrial Microbiology & Biotechnology
PaperScreen-less expanded bed column: new approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris
Trinh, Loc, Phue, Je‐Nie, Jaluria, Pratik, Tsai, Chiawei W., Narum, David L., Shiloach, Joseph · 2006 · Biotechnology Letters · DOI
Full title: Screen-less expanded bed column: new approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris
Authors: Trinh, Loc, Phue, Je‐Nie, Jaluria, Pratik, Tsai, Chiawei W., Narum, David L., Shiloach, Joseph
PaperDerivation of a growth hormone gene cassette for goat by mutagenesis of the corresponding bovine construct and its expression in Pichia pastoris
J. M. Reyes-Ruiz, J. A. Ascacio-Martínez, H. Barrera-Saldana · 2006 · Biotechnology Letters · DOI
Full title: Derivation of a growth hormone gene cassette for goat by mutagenesis of the corresponding bovine construct and its expression in Pichia pastoris
Authors: J. M. Reyes-Ruiz, J. A. Ascacio-Martínez, H. Barrera-Saldana
PaperSubstrate affinity and catalytic efficiency are improved by decreasing glycosylation sites in Trichoderma reesei cellobiohydrolase I expressed in Pichia pastoris.
Full title: Substrate affinity and catalytic efficiency are improved by decreasing glycosylation sites in Trichoderma reesei cellobiohydrolase I expressed in Pichia pastoris.
PaperCharacterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris.
Blanchard, Véronique, Gadkari, Rupali A, Gerwig, Gerrit J, Leeflang, Bas R, Dighe, Rajan R, Kamerling, Johannis P · 2007 · Glycoconjugate journal · DOI
Full title: Characterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris.
Authors: Blanchard, Véronique, Gadkari, Rupali A, Gerwig, Gerrit J, Leeflang, Bas R, Dighe, Rajan R, Kamerling, Johannis P
PaperExpression, purification and characterization of low-glycosylation influenza neuraminidase in α-1,6-mannosyltransferase defective Pichia pastoris.
Yang, Yi-Li, Chang, Shao-Hong, Gong, Xin, Wu, Jun, Liu, Bo · 2012 · Molecular biology reports · DOI
Full title: Expression, purification and characterization of low-glycosylation influenza neuraminidase in α-1,6-mannosyltransferase defective Pichia pastoris.
PaperInfluence of copy number on the expression levels of pandemic influenza hemagglutinin recombinant protein in methylotrophic yeast Pichia pastoris
Athmaram, T. N., Saraswat, Shweta, Singh, Ajay, Rao, M. Kameswara, Gopalan, Natarajan, Suryanarayana, V. V. S., Rao, Ponugoti Vasantha · 2012 · Virus Genes · DOI
Full title: Influence of copy number on the expression levels of pandemic influenza hemagglutinin recombinant protein in methylotrophic yeast Pichia pastoris
Authors: Athmaram, T. N., Saraswat, Shweta, Singh, Ajay, Rao, M. Kameswara, Gopalan, Natarajan, Suryanarayana, V. V. S., Rao, Ponugoti Vasantha
PaperEvaluation of the sub-optimal induction strategies for heterologous proteins production by Pichia pastoris Mut+/MutS strains and related transcriptional and metabolic analysis.
Jia, Luqiang, Gao, Minjie, Yan, Jian, Chen, Shanshan, Sun, Jiaowen, Hua, Qiang, Ding, Jian, Shi, Zhongping · 2018 · World journal of microbiology & biotechnology · DOI
Full title: Evaluation of the sub-optimal induction strategies for heterologous proteins production by Pichia pastoris Mut+/MutS strains and related transcriptional and metabolic analysis.
PaperReconstruction and visualization of carbohydrate, N-glycosylation pathways in Pichia pastoris CBS7435 using computational and system biology approaches
Srivastava, Akriti, Somvanshi, Pallavi, Mishra, Bhartendu Nath · 2012 · Systems and Synthetic Biology · DOI
Full title: Reconstruction and visualization of carbohydrate, N-glycosylation pathways in Pichia pastoris CBS7435 using computational and system biology approaches
PaperBuried Kex2 Sites in Glargine Precursor Aggregates Prevent Its Intracellular Processing in Pichia pastoris Muts Strains and the Effect of Methanol-Feeding Strategy and Induction Temperature on Glargine Precursor Production Parameters
Abel Caballero-Pérez, J. M. Viader-Salvadó, Ana Lucía Herrera-Estala, J. A. Fuentes-Garibay, M. Guerrero-Olazarán · 2021 · Applied Biochemistry and Biotechnology · DOI
Full title: Buried Kex2 Sites in Glargine Precursor Aggregates Prevent Its Intracellular Processing in Pichia pastoris Muts Strains and the Effect of Methanol-Feeding Strategy and Induction Temperature on Glargine Precursor Production Parameters
Authors: Abel Caballero-Pérez, J. M. Viader-Salvadó, Ana Lucía Herrera-Estala, J. A. Fuentes-Garibay, M. Guerrero-Olazarán
PaperPurification, characterization of GH11 endo-β-1,4-xylanase from thermotolerant Streptomyces sp. SWU10 and overexpression in Pichia pastoris KM71H.
Full title: Purification, characterization of GH11 endo-β-1,4-xylanase from thermotolerant Streptomyces sp. SWU10 and overexpression in Pichia pastoris KM71H.
PaperLow-scale expression and purification of an active putative iduronate 2-sulfate sulfatase-Like enzyme from Escherichia coli K12.
Morales-Álvarez, Edwin David, Rivera-Hoyos, Claudia Marcela, Baena-Moncada, Angélica María, Landázuri, Patricia, Poutou-Piñales, Raúl A, Sáenz-Suárez, Homero, Barrera, Luis A, Echeverri-Peña, Olga Y · 2013 · Journal of microbiology (Seoul, Korea) · DOI
Full title: Low-scale expression and purification of an active putative iduronate 2-sulfate sulfatase-Like enzyme from Escherichia coli K12.
Authors: Morales-Álvarez, Edwin David, Rivera-Hoyos, Claudia Marcela, Baena-Moncada, Angélica María, Landázuri, Patricia, Poutou-Piñales, Raúl A, Sáenz-Suárez, Homero, Barrera, Luis A, Echeverri-Peña, Olga Y
PaperExpression of Bacillus licheniformis α-amylase in Pichia pastoris without antibiotics-resistant gene and effects of glycosylation on the enzymic thermostability.
Hu, Xinlin, Yuan, Xin, He, Nisha, Zhuang, Tony Z, Wu, Pan, Zhang, Guimin · 2019 · 3 Biotech · DOI
Full title: Expression of Bacillus licheniformis α-amylase in Pichia pastoris without antibiotics-resistant gene and effects of glycosylation on the enzymic thermostability.
PaperDevelopment and validation of a medium for recombinant endo-β-1,4-xylanase production by Kluyveromyces lactis using a statistical experimental design
Fuzi, Siti Fatimah Zaharah Mohd, Mahadi, Nor Muhammad, Jahim, Jamaliah Md, Murad, Abdul Munir Abdul, Bakar, Farah Diba Abu, Jusoh, Mazura, Rahman, Roshanida A., Illias, Rosli Md · 2011 · Annals of Microbiology · DOI
Full title: Development and validation of a medium for recombinant endo-β-1,4-xylanase production by Kluyveromyces lactis using a statistical experimental design
Authors: Fuzi, Siti Fatimah Zaharah Mohd, Mahadi, Nor Muhammad, Jahim, Jamaliah Md, Murad, Abdul Munir Abdul, Bakar, Farah Diba Abu, Jusoh, Mazura, Rahman, Roshanida A., Illias, Rosli Md
PaperIncrease of Candida antarctica lipase B production under PGK promoter in Pichia pastoris: effect of multicopies
Robert, Julia de Macedo, Betancur, Maritza Ocampo, Machado, Antonio Carlos Oliveira, Arruda, Andrelisse, Reis, Viviane Castelo Branco, Almeida, Rodrigo Volcan, Torres, Fernando Araripe Gonçalves, Alegre, Pau Ferrer i, Valero, Francisco, Freire, Denise Maria Guimarães · 2019 · Brazilian Journal of Microbiology · DOI
Full title: Increase of Candida antarctica lipase B production under PGK promoter in Pichia pastoris: effect of multicopies
Authors: Robert, Julia de Macedo, Betancur, Maritza Ocampo, Machado, Antonio Carlos Oliveira, Arruda, Andrelisse, Reis, Viviane Castelo Branco, Almeida, Rodrigo Volcan, Torres, Fernando Araripe Gonçalves, Alegre, Pau Ferrer i, Valero, Francisco, Freire, Denise Maria Guimarães
Full title: Characterization and application in recombinant N-GlcNAc-protein production of a novel endo-β-N-acetylglucosaminidase from Listeria booriae.
PaperA comparative study of β-1, 4-endoglucanase (possessing β-1, 4-exoglucanase activity) from Bacillus subtilis LH expressed in Pichia pastoris GS115 and Escherichia coli Rosetta (DE3).
Full title: A comparative study of β-1, 4-endoglucanase (possessing β-1, 4-exoglucanase activity) from Bacillus subtilis LH expressed in Pichia pastoris GS115 and Escherichia coli Rosetta (DE3).
PaperGeneration of xylooligosaccharides from microwave irradiated agroresidues using recombinant thermo-alkali-stable endoxylanase of the polyextremophilic bacterium Bacillus halodurans expressed in Pichia pastoris.
Kumar, Vikash, Satyanarayana, T · 2015 · Bioresource technology · DOI
Full title: Generation of xylooligosaccharides from microwave irradiated agroresidues using recombinant thermo-alkali-stable endoxylanase of the polyextremophilic bacterium Bacillus halodurans expressed in Pichia pastoris.
PaperNovel multimodal cation-exchange membrane for the purification of a single-chain variable fragment from Pichia pastoris supernatant
Pham, Dan N., Linova, Marina Y., Smith, William K., Brown, Hunter, Elhanafi, Driss, Fan, Jinxin, Lavoie, Joseph, Woodley, John M., Carbonell, Ruben G. · 2024 · Journal of Chromatography A · DOI
Full title: Novel multimodal cation-exchange membrane for the purification of a single-chain variable fragment from Pichia pastoris supernatant
Authors: Pham, Dan N., Linova, Marina Y., Smith, William K., Brown, Hunter, Elhanafi, Driss, Fan, Jinxin, Lavoie, Joseph, Woodley, John M., Carbonell, Ruben G.
PaperHigh-yield production of the human lysozyme by Pichia pastoris SMD1168 using response surface methodology and high-cell-density fermentation
Yu, Ying, Zhou, Xiaoyu, Wu, Sheng, Wei, Tiantian, Yu, Long · 2014 · Electronic Journal of Biotechnology · DOI
Full title: High-yield production of the human lysozyme by Pichia pastoris SMD1168 using response surface methodology and high-cell-density fermentation
PaperA novel feeding strategy during the production phase for enhancing the enzymatic synthesis of S-adenosyl-l-methionine by methylotrophic Pichia pastoris
Full title: A novel feeding strategy during the production phase for enhancing the enzymatic synthesis of S-adenosyl-l-methionine by methylotrophic Pichia pastoris
PaperOptimization of the heterologous production of a Rhizopus oryzae lipase in Pichia pastoris system using mixed substrates on controlled fed-batch bioprocess
Arnau, Carolina, Ramón, Ramón, Casas, C., Valero, Francisco · 2010 · Enzyme and Microbial Technology · DOI
Full title: Optimization of the heterologous production of a Rhizopus oryzae lipase in Pichia pastoris system using mixed substrates on controlled fed-batch bioprocess
Authors: Arnau, Carolina, Ramón, Ramón, Casas, C., Valero, Francisco
PaperImprovement of catalytical properties of two invertases highly tolerant to sucrose after expression in Pichia pastoris. Effect of glycosylation on enzyme properties.
Pérez de los Santos, Ara Itzel, Cayetano-Cruz, Maribel, Gutiérrez-Antón, Marina, Santiago-Hernández, Alejandro, Plascencia-Espinosa, Miguel, Farrés, Amelia, Hidalgo-Lara, María Eugenia · 2016 · Enzyme and microbial technology · DOI
Full title: Improvement of catalytical properties of two invertases highly tolerant to sucrose after expression in Pichia pastoris. Effect of glycosylation on enzyme properties.
Authors: Pérez de los Santos, Ara Itzel, Cayetano-Cruz, Maribel, Gutiérrez-Antón, Marina, Santiago-Hernández, Alejandro, Plascencia-Espinosa, Miguel, Farrés, Amelia, Hidalgo-Lara, María Eugenia
PaperGenome mining and motif truncation of glycoside hydrolase family 5 endo-β-1,4-mannanase encoded by Aspergillus oryzae RIB40 for potential konjac flour hydrolysis or feed additive.
Full title: Genome mining and motif truncation of glycoside hydrolase family 5 endo-β-1,4-mannanase encoded by Aspergillus oryzae RIB40 for potential konjac flour hydrolysis or feed additive.
PaperExpression of a novel α-glucosidase from Aspergillus neoniger in Pichia pastoris and its efficient recovery for synthesis of isomaltooligosaccharides.
Kumar, Sandeep, Mutturi, Sarma · 2020 · Enzyme and microbial technology · DOI
Full title: Expression of a novel α-glucosidase from Aspergillus neoniger in Pichia pastoris and its efficient recovery for synthesis of isomaltooligosaccharides.
PaperComparative characterization of glyoxal oxidase from Phanerochaete chrysosporium expressed at high levels in Pichia pastoris and Trichoderma reesei.
Wohlschlager, Lena, Csarman, Florian, Zrilić, Matea, Seiboth, Bernhard, Ludwig, Roland · 2021 · Enzyme and microbial technology · DOI
Full title: Comparative characterization of glyoxal oxidase from Phanerochaete chrysosporium expressed at high levels in Pichia pastoris and Trichoderma reesei.
PaperDisrupting putative N-glycosylation site N17 in lipase Lip11 of Yarrowia lipolytica yielded a catalytically efficient and thermostable variant accompanying conformational changes.
Kashyap, Amuliya, Gupta, Rani · 2021 · Enzyme and microbial technology · DOI
Full title: Disrupting putative N-glycosylation site N17 in lipase Lip11 of Yarrowia lipolytica yielded a catalytically efficient and thermostable variant accompanying conformational changes.
PaperThe 3rd Worldwide Network Conference (HPWN-3): ?Strengthening the connection between fundamental research and its biotechnological applications?, Alghero, Sardinia, Italy, 25?29 September 2004
Kiel, Jan A.K.W., DeBoer, Arthur · 2005 · FEMS Yeast Research · DOI
Full title: The 3rd Worldwide Network Conference (HPWN-3): ?Strengthening the connection between fundamental research and its biotechnological applications?, Alghero, Sardinia, Italy, 25?29 September 2004
PaperA novel β-fructofuranosidase in Coleoptera: Characterization of a β-fructofuranosidase from the sugarcane weevil, Sphenophorus levis.
Pedezzi, Rafael, Fonseca, Fernando P P, Santos Júnior, Célio Dias, Kishi, Luciano T, Terra, Walter R, Henrique-Silva, Flávio · 2014 · Insect biochemistry and molecular biology · DOI
Full title: A novel β-fructofuranosidase in Coleoptera: Characterization of a β-fructofuranosidase from the sugarcane weevil, Sphenophorus levis.
Authors: Pedezzi, Rafael, Fonseca, Fernando P P, Santos Júnior, Célio Dias, Kishi, Luciano T, Terra, Walter R, Henrique-Silva, Flávio
PaperHeterologous expression and biochemical characterization of a novel thermostable Sclerotinia sclerotiorum GH45 endoglucanase in Pichia pastoris.
Chahed, Haifa, Boumaiza, Mohamed, Ezzine, Aymen, Marzouki, M Nejib · 2018 · International journal of biological macromolecules · DOI
Full title: Heterologous expression and biochemical characterization of a novel thermostable Sclerotinia sclerotiorum GH45 endoglucanase in Pichia pastoris.
Authors: Chahed, Haifa, Boumaiza, Mohamed, Ezzine, Aymen, Marzouki, M Nejib
Year: 2018
Venue: International journal of biological macromolecules
PaperCombined effect of gene dosage and process optimization strategies on high-level production of recombinant human interleukin-3 (hIL-3) in Pichia pastoris fed-batch culture.
Dagar, Vikas Kumar, Khasa, Yogender Pal · 2018 · International journal of biological macromolecules · DOI
Full title: Combined effect of gene dosage and process optimization strategies on high-level production of recombinant human interleukin-3 (hIL-3) in Pichia pastoris fed-batch culture.
Authors: Dagar, Vikas Kumar, Khasa, Yogender Pal
Year: 2018
Venue: International journal of biological macromolecules
PaperHeterologous expression and characterization of an antifungal chitinase (Chit46) from Trichoderma harzianum GIM 3.442 and its application in colloidal chitin conversion.
Deng, Jun-Jin, Shi, Dan, Mao, He-Hua, Li, Zhi-Wei, Liang, Shuang, Ke, Ye, Luo, Xiao-Chun · 2019 · International journal of biological macromolecules · DOI
Full title: Heterologous expression and characterization of an antifungal chitinase (Chit46) from Trichoderma harzianum GIM 3.442 and its application in colloidal chitin conversion.
Authors: Deng, Jun-Jin, Shi, Dan, Mao, He-Hua, Li, Zhi-Wei, Liang, Shuang, Ke, Ye, Luo, Xiao-Chun
Year: 2019
Venue: International journal of biological macromolecules
PaperEffects of N-linked glycosylation on the enzymatic properties of GH12 bifunctional enzymes from Aspergillus terreus expressed in Pichia pastoris.
Zuo, Dinghui, He, Jinjian, Sun, Xihang, Liu, Hanting, Xia, Shenju, Wang, Mansheng, Zheng, Xia, Liu, Huiqin, Shi, Pengjun · 2025 · International journal of biological macromolecules · DOI
Full title: Effects of N-linked glycosylation on the enzymatic properties of GH12 bifunctional enzymes from Aspergillus terreus expressed in Pichia pastoris.
PaperDeglycosylation significantly affects the activity, stability and appropriate folding of recombinant Aspergillus niger α-L-rhamnosidase expressed in Pichia pastoris.
Lu, Qihui, Liao, Hui, Jiang, Zedong, Zhu, Yanbing, Han, Yijuan, Li, Lijun, Ni, Hui, Li, Qingbiao · 2025 · International journal of biological macromolecules · DOI
Full title: Deglycosylation significantly affects the activity, stability and appropriate folding of recombinant Aspergillus niger α-L-rhamnosidase expressed in Pichia pastoris.
PaperCell-associated beta-xylosidase from Aureobasidium pullulans ATCC 20524: Purification, properties, and characterization of the encoding gene.
Ohta, Kazuyoshi, Fujimoto, Hirohisa, Fujii, Shinya, Wakiyama, Motoki · 2010 · Journal of bioscience and bioengineering · DOI
Full title: Cell-associated beta-xylosidase from Aureobasidium pullulans ATCC 20524: Purification, properties, and characterization of the encoding gene.
PaperCharacterization of a glycoside hydrolase family-51 α-l-arabinofuranosidase gene from Aureobasidium pullulans ATCC 20524 and its encoded product.
Ohta, Kazuyoshi, Fujii, Shinya, Higashida, Chihiro · 2013 · Journal of bioscience and bioengineering · DOI
Full title: Characterization of a glycoside hydrolase family-51 α-l-arabinofuranosidase gene from Aureobasidium pullulans ATCC 20524 and its encoded product.
PaperMinimization of aggregation of secreted bivalent anti-human T cell immunotoxin in Pichia pastoris bioreactor culture by optimizing culture conditions for protein secretion.
Woo, Jung Hee, Liu, Yuan Yi, Neville, David M · 2006 · Journal of biotechnology · DOI
Full title: Minimization of aggregation of secreted bivalent anti-human T cell immunotoxin in Pichia pastoris bioreactor culture by optimizing culture conditions for protein secretion.
Authors: Woo, Jung Hee, Liu, Yuan Yi, Neville, David M
PaperMixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures.
Jungo, Carmen, Marison, Ian, von Stockar, Urs · 2007 · Journal of biotechnology · DOI
Full title: Mixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures.
Authors: Jungo, Carmen, Marison, Ian, von Stockar, Urs
PaperA quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris.
Jungo, Carmen, Schenk, Jonas, Pasquier, Muriel, Marison, Ian William, von Stockar, Urs · 2007 · Journal of biotechnology · DOI
Full title: A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris.
Authors: Jungo, Carmen, Schenk, Jonas, Pasquier, Muriel, Marison, Ian William, von Stockar, Urs
PaperProduction of monoclonal antibodies by glycoengineered Pichia pastoris.
Potgieter, Thomas I, Cukan, Michael, Drummond, James E, Houston-Cummings, Nga Rewa, Jiang, Youwei, Li, Fang, Lynaugh, Heather, Mallem, Muralidhar, McKelvey, Troy W, Mitchell, Teresa, Nylen, Adam, Rittenhour, Alissa, Stadheim, Terrance A, Zha, Dongxing, d'Anjou, Marc · 2009 · Journal of biotechnology · DOI
Full title: Production of monoclonal antibodies by glycoengineered Pichia pastoris.
Authors: Potgieter, Thomas I, Cukan, Michael, Drummond, James E, Houston-Cummings, Nga Rewa, Jiang, Youwei, Li, Fang, Lynaugh, Heather, Mallem, Muralidhar, McKelvey, Troy W, Mitchell, Teresa, Nylen, Adam, Rittenhour, Alissa, Stadheim, Terrance A, Zha, Dongxing, d'Anjou, Marc
PaperOver expression and analysis of O-glycosylated recombinant human granulocyte colony stimulating factor in Pichia pastoris using Agilent 2100 Bioanalyzer.
Apte-Deshpande, Anjali, Somani, Sandeep, Mandal, Goutam, Soorapaneni, Sudheerbabu, Padmanabhan, Sriram · 2009 · Journal of biotechnology · DOI
Full title: Over expression and analysis of O-glycosylated recombinant human granulocyte colony stimulating factor in Pichia pastoris using Agilent 2100 Bioanalyzer.
PaperFructooligosaccharides production by Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase constitutively expressed to high levels in Pichia pastoris.
Hernández, Lázaro, Menéndez, Carmen, Pérez, Enrique R, Martínez, Duniesky, Alfonso, Dubiel, Trujillo, Luis E, Ramírez, Ricardo, Sobrino, Alina, Mazola, Yuliet, Musacchio, Alexis, Pimentel, Eulogio · 2018 · Journal of biotechnology · DOI
Full title: Fructooligosaccharides production by Schedonorus arundinaceus sucrose:sucrose 1-fructosyltransferase constitutively expressed to high levels in Pichia pastoris.
PaperCo-feeding strategy alleviates hypoxic stress in large-scale bioreactor for optimal production of secretory recombinant proteins in Pichia pastoris.
Velastegui, Edgar, Quezada, Johan, Altamirano, Claudia, Berrios, Julio, Fickers, Patrick · 2023 · Journal of biotechnology · DOI
Full title: Co-feeding strategy alleviates hypoxic stress in large-scale bioreactor for optimal production of secretory recombinant proteins in Pichia pastoris.
PaperAn optimized fermentation process for high-level production of a single-chain Fv antibody fragment in Pichia pastoris.
Damasceno, Leonardo M, Pla, Itzcoatl, Chang, Hyun-Joo, Cohen, Leonard, Ritter, Gerd, Old, Lloyd J, Batt, Carl A · 2004 · Protein expression and purification · DOI
Full title: An optimized fermentation process for high-level production of a single-chain Fv antibody fragment in Pichia pastoris.
Authors: Damasceno, Leonardo M, Pla, Itzcoatl, Chang, Hyun-Joo, Cohen, Leonard, Ritter, Gerd, Old, Lloyd J, Batt, Carl A
PaperHigh-level expression, purification, and characterization of recombinant wheat xylanase inhibitor TAXI-I secreted by the yeast Pichia pastoris.
Fierens, Katleen, Geudens, Nele, Brijs, Kristof, Courtin, Christophe M, Gebruers, Kurt, Robben, Johan, Van Campenhout, Steven, Volckaert, Guido, Delcour, Jan A · 2004 · Protein expression and purification · DOI
Full title: High-level expression, purification, and characterization of recombinant wheat xylanase inhibitor TAXI-I secreted by the yeast Pichia pastoris.
Authors: Fierens, Katleen, Geudens, Nele, Brijs, Kristof, Courtin, Christophe M, Gebruers, Kurt, Robben, Johan, Van Campenhout, Steven, Volckaert, Guido, Delcour, Jan A
PaperA combined approach for improving alkaline acetyl xylan esterase production in Pichia pastoris, and effects of glycosylation on enzyme secretion, activity and stability.
Tian, Bin, Chen, Yan, Ding, Shaojun · 2012 · Protein expression and purification · DOI
Full title: A combined approach for improving alkaline acetyl xylan esterase production in Pichia pastoris, and effects of glycosylation on enzyme secretion, activity and stability.
PaperDesign, production, and characterization of a single-chain variable fragment (ScFv) derived from the prostate specific membrane antigen (PSMA) monoclonal antibody J591.
Parker, Stephanie A, Diaz, Ines Lopez-Calleja, Anderson, Kyle A, Batt, Carl A · 2013 · Protein expression and purification · DOI
Full title: Design, production, and characterization of a single-chain variable fragment (ScFv) derived from the prostate specific membrane antigen (PSMA) monoclonal antibody J591.
Authors: Parker, Stephanie A, Diaz, Ines Lopez-Calleja, Anderson, Kyle A, Batt, Carl A
PaperBiochemical properties of glycosylation and characterization of a histidine acid phosphatase (phytase) expressed in Pichia pastoris.
Fonseca-Maldonado, Raquel, Maller, Alexandre, Bonneil, Eric, Thibault, Pierre, Botelho-Machado, Carla, Ward, Richard John, Polizeli, Maria de Lourdes Teixeira de Moraes · 2014 · Protein expression and purification · DOI
Full title: Biochemical properties of glycosylation and characterization of a histidine acid phosphatase (phytase) expressed in Pichia pastoris.
Authors: Fonseca-Maldonado, Raquel, Maller, Alexandre, Bonneil, Eric, Thibault, Pierre, Botelho-Machado, Carla, Ward, Richard John, Polizeli, Maria de Lourdes Teixeira de Moraes
PaperSaccharomyces cerevisiae asparaginase II, a potential antileukemic drug: Purification and characterization of the enzyme expressed in Pichia pastoris.
Facchinetti de Castro Girão, Luciana, Gonçalves da Rocha, Surza Lucia, Sobral, Ricardo Sposina, Dinis Ano Bom, Ana Paula, Franco Sampaio, André Luiz, Godinho da Silva, José, Ferrara, Maria Antonieta, Pinto da Silva Bon, Elba, Perales, Jonas · 2016 · Protein expression and purification · DOI
Full title: Saccharomyces cerevisiae asparaginase II, a potential antileukemic drug: Purification and characterization of the enzyme expressed in Pichia pastoris.
Authors: Facchinetti de Castro Girão, Luciana, Gonçalves da Rocha, Surza Lucia, Sobral, Ricardo Sposina, Dinis Ano Bom, Ana Paula, Franco Sampaio, André Luiz, Godinho da Silva, José, Ferrara, Maria Antonieta, Pinto da Silva Bon, Elba, Perales, Jonas
PaperOptimized expression of the Starmerella bombicola lactone esterase in Pichia pastoris through temperature adaptation, codon-optimization and co-expression with HAC1
Waele, Stijn De, Vandenberghe, Isabel, Laukens, Bram, Planckaert, Sören, Verweire, Stijn, Bogaert, Inge Van, Soetaert, Wim, Devreese, Bart, Ciesielska, Katarzyna · 2017 · Protein Expression and Purification · DOI
Full title: Optimized expression of the Starmerella bombicola lactone esterase in Pichia pastoris through temperature adaptation, codon-optimization and co-expression with HAC1
Authors: Waele, Stijn De, Vandenberghe, Isabel, Laukens, Bram, Planckaert, Sören, Verweire, Stijn, Bogaert, Inge Van, Soetaert, Wim, Devreese, Bart, Ciesielska, Katarzyna
PaperDownstream process and evaluation of the concomitant impact of a recombinant glycosylated L-asparaginase on leukemic cancer cells and the bone marrow tumor microenvironment
Kleingesinds, Eduardo Krebs, Parizotto, Letícia de Almeida, Effer, Brian, Monteiro, Gisele, Long, Paul F., Arroyo-Berdugo, Yoana, Behrends, Volker, Esposito, Maria Teresa, Calle, Yolanda, Pessoa, Adalberto · 2023 · Process Biochemistry · DOI
Full title: Downstream process and evaluation of the concomitant impact of a recombinant glycosylated L-asparaginase on leukemic cancer cells and the bone marrow tumor microenvironment
Authors: Kleingesinds, Eduardo Krebs, Parizotto, Letícia de Almeida, Effer, Brian, Monteiro, Gisele, Long, Paul F., Arroyo-Berdugo, Yoana, Behrends, Volker, Esposito, Maria Teresa, Calle, Yolanda, Pessoa, Adalberto
PaperActive site mapping, biochemical properties and subcellular localization of rhodesain, the major cysteine protease of Trypanosoma brucei rhodesiense.
Caffrey, C R, Hansell, E, Lucas, K D, Brinen, L S, Alvarez Hernandez, A, Cheng, J, Gwaltney, S L, Roush, W R, Stierhof, Y D, Bogyo, M, Steverding, D, McKerrow, J H · 2001 · Molecular and biochemical parasitology · DOI
Full title: Active site mapping, biochemical properties and subcellular localization of rhodesain, the major cysteine protease of Trypanosoma brucei rhodesiense.
Authors: Caffrey, C R, Hansell, E, Lucas, K D, Brinen, L S, Alvarez Hernandez, A, Cheng, J, Gwaltney, S L, Roush, W R, Stierhof, Y D, Bogyo, M, Steverding, D, McKerrow, J H
PaperExpression and characterization of a synthetic protein C activator in Pichia pastoris.
Kunes, Yune Zhang, Sanz, María-Cruz, Tumanova, Irina, Birr, Cynthia A, Shi, Philip Q, Bruguera, Pau, Ruiz, Juan A, Sánchez-Martínez, Demetrio · 2002 · Protein expression and purification · DOI
Full title: Expression and characterization of a synthetic protein C activator in Pichia pastoris.
Authors: Kunes, Yune Zhang, Sanz, María-Cruz, Tumanova, Irina, Birr, Cynthia A, Shi, Philip Q, Bruguera, Pau, Ruiz, Juan A, Sánchez-Martínez, Demetrio
PaperStabilizing Leaf and Branch Compost Cutinase (LCC) with Glycosylation: Mechanism and Effect on PET Hydrolysis.
Shirke, Abhijit N, White, Christine, Englaender, Jacob A, Zwarycz, Allison, Butterfoss, Glenn L, Linhardt, Robert J, Gross, Richard A · 2018 · Biochemistry · DOI
Full title: Stabilizing Leaf and Branch Compost Cutinase (LCC) with Glycosylation: Mechanism and Effect on PET Hydrolysis.
Authors: Shirke, Abhijit N, White, Christine, Englaender, Jacob A, Zwarycz, Allison, Butterfoss, Glenn L, Linhardt, Robert J, Gross, Richard A
Full title: Structural and Biochemical Studies Enlighten the Unspecific Peroxygenase from <i>Hypoxylon</i> sp. EC38 as an Efficient Oxidative Biocatalyst
PaperImproving the Catalytic Efficiency of Aspergillus fumigatus Glucoamylase toward Raw Starch by Engineering Its N-Glycosylation Sites and Saturation Mutation.
Song, Weiyan, Li, Yangyang, Tong, Yi, Li, Yi, Tao, Jin, Rao, Shengqi, Li, Jianghua, Zhou, Jingwen, Liu, Song · 2022 · Journal of agricultural and food chemistry · DOI
Full title: Improving the Catalytic Efficiency of Aspergillus fumigatus Glucoamylase toward Raw Starch by Engineering Its N-Glycosylation Sites and Saturation Mutation.
PaperHuman chymotrypsinogen B production with Pichia pastoris by integrated development of fermentation and downstream processing. Part 1. Fermentation.
Full title: Human chymotrypsinogen B production with Pichia pastoris by integrated development of fermentation and downstream processing. Part 1. Fermentation.
PaperMolecular characterization of a thermophilic endo-polygalacturonase from Thielavia arenaria XZ7 with high catalytic efficiency and application potential in the food and feed industries.
Tu, Tao, Meng, Kun, Huang, Huoqing, Luo, Huiying, Bai, Yingguo, Ma, Rui, Su, Xiaoyun, Shi, Pengjun, Yang, Peilong, Wang, Yaru, Yao, Bin · 2014 · Journal of agricultural and food chemistry · DOI
Full title: Molecular characterization of a thermophilic endo-polygalacturonase from Thielavia arenaria XZ7 with high catalytic efficiency and application potential in the food and feed industries.
PaperEstablishing a versatile fermentation and purification procedure for human proteins expressed in the yeasts Saccharomyces cerevisiae and Pichia pastoris for structural genomics
Prinz, Bianka, Schultchen, Jeffrey, Rydzewski, Ralf, Holz, Caterina, Boettner, Mewes, Ståhl, Ulf, Lang, Christine · 2004 · Journal of Structural and Functional Genomics · DOI
Full title: Establishing a versatile fermentation and purification procedure for human proteins expressed in the yeasts Saccharomyces cerevisiae and Pichia pastoris for structural genomics
PaperPrevention of human granulocyte colony-stimulating factor protein aggregation in recombinant Pichia pastoris fed-batch fermentation using additives.
Bahrami, Ali, Shojaosadati, Seyed Abbas, Khalilzadeh, Rasoul, Mohammadian, Jafar, Farahani, Ebrahim Vashghani, Masoumian, Mohammad Reza · 2009 · Biotechnology and applied biochemistry · DOI
Full title: Prevention of human granulocyte colony-stimulating factor protein aggregation in recombinant Pichia pastoris fed-batch fermentation using additives.
PaperSecondary structure, conformational stability and glycosylation of a recombinant Candida rugosa lipase studied by Fourier-transform infrared spectroscopy.
Natalello, Antonino, Ami, Diletta, Brocca, Stefania, Lotti, Marina, Doglia, Silvia M · 2005 · The Biochemical journal · DOI
Full title: Secondary structure, conformational stability and glycosylation of a recombinant Candida rugosa lipase studied by Fourier-transform infrared spectroscopy.
PaperG-protein coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent
Jamshad, Mohammed, Charlton, Jack, Lin, Yu-Pin, Routledge, Sarah J, Bawa, Zharain, Knowles, Timothy J., Overduin, Michael, Dekker, Niek, Dafforn, Timothy R., Bill, Roslyn M., Poyner, David R., Wheatley, Mark · 2015 · Bioscience Reports · DOI
Full title: G-protein coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent
Authors: Jamshad, Mohammed, Charlton, Jack, Lin, Yu-Pin, Routledge, Sarah J, Bawa, Zharain, Knowles, Timothy J., Overduin, Michael, Dekker, Niek, Dafforn, Timothy R., Bill, Roslyn M., Poyner, David R., Wheatley, Mark
PaperUnexpected presence of fructan 6‐exohydrolases (6‐FEHs) in non‐fructan plants: characterization, cloning, mass mapping and functional analysis of a novel ‘cell‐wall invertase‐like’ specific 6‐FEH from sugar beet (<i>Beta vulgaris</i> L.)
Ende, Wim Van den, Coninck, Barbara De, Clerens, Stefan, Vergauwen, Rudy, Laere, André Van · 2003 · The Plant Journal · DOI
Full title: Unexpected presence of fructan 6‐exohydrolases (6‐FEHs) in non‐fructan plants: characterization, cloning, mass mapping and functional analysis of a novel ‘cell‐wall invertase‐like’ specific 6‐FEH from sugar beet (<i>Beta vulgaris</i> L.)
Authors: Ende, Wim Van den, Coninck, Barbara De, Clerens, Stefan, Vergauwen, Rudy, Laere, André Van
PaperProtection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutinin.
Saelens, X, Vanlandschoot, P, Martinet, W, Maras, M, Neirynck, S, Contreras, R, Fiers, W, Jou, W M · 1999 · European journal of biochemistry · DOI
Full title: Protection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutinin.
Authors: Saelens, X, Vanlandschoot, P, Martinet, W, Maras, M, Neirynck, S, Contreras, R, Fiers, W, Jou, W M
PaperEngineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice
Dalvie, Neil C., Rodriguez‐Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Célia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dong Soo, Brunette, Natalie, Wang, Jing Yang, Walkey, Carl, Fiala, Brooke, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanné, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith M., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice, Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher · 2021 · Proceedings of the National Academy of Sciences · DOI
Full title: Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice
Authors: Dalvie, Neil C., Rodriguez‐Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Célia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dong Soo, Brunette, Natalie, Wang, Jing Yang, Walkey, Carl, Fiala, Brooke, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanné, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith M., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice, Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher
Year: 2021
Venue: Proceedings of the National Academy of Sciences
PaperIntracellular Expression of a Single Domain Antibody Reduces Cytotoxicity of 15-Acetyldeoxynivalenol in Yeast
Doyle, Patrick J., Saeed, Hanaa, Hermans, Anne, Gleddie, Steve, Hussack, Greg, Arbabi‐Ghahroudi, Mehdi, Seguin, Charles, Savard, Marc E., MacKenzie, C. Roger, Hall, J. Christopher · 2009 · Journal of Biological Chemistry · DOI
Full title: Intracellular Expression of a Single Domain Antibody Reduces Cytotoxicity of 15-Acetyldeoxynivalenol in Yeast
Authors: Doyle, Patrick J., Saeed, Hanaa, Hermans, Anne, Gleddie, Steve, Hussack, Greg, Arbabi‐Ghahroudi, Mehdi, Seguin, Charles, Savard, Marc E., MacKenzie, C. Roger, Hall, J. Christopher
PaperGlycosylation in a mammalian expression system is critical for the production of functionally active leukocyte immunoglobulin-like receptor A3 protein.
Lee, Terry H Y, Mitchell, Ainslie, Liu Lau, Sydney, An, Hongyan, Rajeaskariah, Poornima, Wasinger, Valerie, Raftery, Mark, Bryant, Katherine, Tedla, Nicodemus · 2013 · The Journal of biological chemistry · DOI
Full title: Glycosylation in a mammalian expression system is critical for the production of functionally active leukocyte immunoglobulin-like receptor A3 protein.
Authors: Lee, Terry H Y, Mitchell, Ainslie, Liu Lau, Sydney, An, Hongyan, Rajeaskariah, Poornima, Wasinger, Valerie, Raftery, Mark, Bryant, Katherine, Tedla, Nicodemus
PaperSaposin A mobilizes lipids from low cholesterol and high bis(monoacylglycerol)phosphate-containing membranes: patient variant Saposin A lacks lipid extraction capacity.
Locatelli-Hoops, Silvia, Remmel, Natascha, Klingenstein, Ralf, Breiden, Bernadette, Rossocha, Maksim, Schoeniger, Maike, Koenigs, Christine, Saenger, Wolfram, Sandhoff, Konrad · 2006 · The Journal of biological chemistry · DOI
Full title: Saposin A mobilizes lipids from low cholesterol and high bis(monoacylglycerol)phosphate-containing membranes: patient variant Saposin A lacks lipid extraction capacity.
PaperCloning, large-scale production and characterization of fusion protein (P-TUFT-ALT-2) of Brugian abundant larval transcript-2 with tuftsin in Pichia pastoris.
Full title: Cloning, large-scale production and characterization of fusion protein (P-TUFT-ALT-2) of Brugian abundant larval transcript-2 with tuftsin in Pichia pastoris.
PaperRecombinant protein production in <i>Pichia pastoris</i>: from transcriptionally redesigned strains to bioprocess optimization and metabolic modelling
Ergün, Burcu Gündüz, Berríos, Julio, Bi̇nay, Barış, Fickers, Patrick · 2021 · FEMS Yeast Research · DOI
Full title: Recombinant protein production in <i>Pichia pastoris</i>: from transcriptionally redesigned strains to bioprocess optimization and metabolic modelling
Authors: Ergün, Burcu Gündüz, Berríos, Julio, Bi̇nay, Barış, Fickers, Patrick
PaperMolecular characterization of acidic peptide:N-glycanase from the dimorphic yeast Yarrowia lipolytica.
Lee, Kyung Jin, Gil, Jin Young, Kim, Sang-Yoon, Kwon, Ohsuk, Ko, Kisung, Kim, Dong-Il, Kim, Dae Kyong, Kim, Ha Hyung, Oh, Doo-Byoung · 2015 · Journal of biochemistry · DOI
Full title: Molecular characterization of acidic peptide:N-glycanase from the dimorphic yeast Yarrowia lipolytica.
Authors: Lee, Kyung Jin, Gil, Jin Young, Kim, Sang-Yoon, Kwon, Ohsuk, Ko, Kisung, Kim, Dong-Il, Kim, Dae Kyong, Kim, Ha Hyung, Oh, Doo-Byoung
PaperA multi-plex protein expression system for production of complex enzyme formulations in <i>Trichoderma reesei</i>
Subramanian, Venkataramanan, Farmer, Samuel J., Heiland, Kelsey L, Moore, Kyle T., Wall, Todd A. Vander, Sun, Weiman, Chaudhari, Yogesh B., Himmel, Michael E., Decker, Stephen R. · 2022 · Journal of Industrial Microbiology & Biotechnology · DOI
Full title: A multi-plex protein expression system for production of complex enzyme formulations in <i>Trichoderma reesei</i>
Authors: Subramanian, Venkataramanan, Farmer, Samuel J., Heiland, Kelsey L, Moore, Kyle T., Wall, Todd A. Vander, Sun, Weiman, Chaudhari, Yogesh B., Himmel, Michael E., Decker, Stephen R.
Year: 2022
Venue: Journal of Industrial Microbiology & Biotechnology
PaperAnalysis of a xyloglucan endotransglycosylase/hydrolase (XTH) from the lycopodiophyte Selaginella kraussiana suggests that XTH sequence characteristics and function are highly conserved during the evolution of vascular plants.
Van Sandt, Vicky S T, Guisez, Yves, Verbelen, Jean-Pierre, Vissenberg, Kris · 2006 · Journal of experimental botany · DOI
Full title: Analysis of a xyloglucan endotransglycosylase/hydrolase (XTH) from the lycopodiophyte Selaginella kraussiana suggests that XTH sequence characteristics and function are highly conserved during the evolution of vascular plants.
Authors: Van Sandt, Vicky S T, Guisez, Yves, Verbelen, Jean-Pierre, Vissenberg, Kris
PaperCrystallization and preliminary characterization of three different crystal forms of human saposin C heterologously expressed in<i>Pichia pastoris</i>
Schultz‐Heienbrok, Robert, Remmel, Natascha, Klingenstein, Ralf, Rossocha, Maksim, Sandhoff, Konrad, Saenger, Wolfram, Maier, Timm · 2006 · Acta Crystallographica Section F Structural Biology and Crystallization Communications · DOI
Full title: Crystallization and preliminary characterization of three different crystal forms of human saposin C heterologously expressed in<i>Pichia pastoris</i>
PaperExpression, purification, crystallization and preliminary X-ray diffraction analysis of the pectin methylesterase from the sugar cane weevil<i>Sphenophorus levis</i>
Evangelista, Danilo Elton, Godoy, André S., Paula, Fernando Fonseca Pereira de, Henrique‐Silva, Flávio, Polikarpov, Igor · 2014 · Acta Crystallographica Section F Structural Biology Communications · DOI
Full title: Expression, purification, crystallization and preliminary X-ray diffraction analysis of the pectin methylesterase from the sugar cane weevil<i>Sphenophorus levis</i>
Authors: Evangelista, Danilo Elton, Godoy, André S., Paula, Fernando Fonseca Pereira de, Henrique‐Silva, Flávio, Polikarpov, Igor
Year: 2014
Venue: Acta Crystallographica Section F Structural Biology Communications
PaperExpression, purification, crystallization and crystallographic study of<i>Lutzomyia longipalpis</i>LJL143
Kelleher, Alan, Liu, Zhuyun, Seid, Christopher A., Zhan, Bin, Asojo, Oluwatoyin A. · 2015 · Acta Crystallographica Section F Structural Biology Communications · DOI
Full title: Expression, purification, crystallization and crystallographic study of<i>Lutzomyia longipalpis</i>LJL143
Authors: Kelleher, Alan, Liu, Zhuyun, Seid, Christopher A., Zhan, Bin, Asojo, Oluwatoyin A.
Year: 2015
Venue: Acta Crystallographica Section F Structural Biology Communications
PaperFine‐tuning the <i>P. pastoris</i> iMT1026 genome‐scale metabolic model for improved prediction of growth on methanol or glycerol as sole carbon sources
Tomàs‐Gamisans, Màrius, Ferrer, Pau, Albiol, Joan · 2017 · Microbial Biotechnology · DOI
Full title: Fine‐tuning the <i>P. pastoris</i> iMT1026 genome‐scale metabolic model for improved prediction of growth on methanol or glycerol as sole carbon sources
Authors: Tomàs‐Gamisans, Màrius, Ferrer, Pau, Albiol, Joan
PaperDeveloping a metabolic model-based fed-batch feeding strategy for Pichia pastoris fermentation through fine-tuning of the methanol utilization pathway.
Boojari, Mohammad Amin, Rajabi Ghaledari, Fatemeh, Motamedian, Ehsan, Soleimani, Mehdi, Shojaosadati, Seyed Abbas · 2023 · Microbial biotechnology · DOI
Full title: Developing a metabolic model-based fed-batch feeding strategy for Pichia pastoris fermentation through fine-tuning of the methanol utilization pathway.
Authors: Boojari, Mohammad Amin, Rajabi Ghaledari, Fatemeh, Motamedian, Ehsan, Soleimani, Mehdi, Shojaosadati, Seyed Abbas
PaperOptimization of recombinant fungal laccase production with strains of the yeast<i>Kluyveromyces lactis</i>âfrom the pyruvate decarboxylase promoter
Ranieri, Danilo, Colao, Maria Chiara, Ruzzi, Maurizio, Romagnoli, Gabriele, Bianchi, Michele M. · 2009 · FEMS Yeast Research · DOI
Full title: Optimization of recombinant fungal laccase production with strains of the yeast<i>Kluyveromyces lactis</i>âfrom the pyruvate decarboxylase promoter
Authors: Ranieri, Danilo, Colao, Maria Chiara, Ruzzi, Maurizio, Romagnoli, Gabriele, Bianchi, Michele M.
PaperRecombinant expression of ShPI-1A, a non-specific BPTI-Kunitz-type inhibitor, and its protection effect on proteolytic degradation of recombinant human miniproinsulin expressed in Pichia pastoris
Gil, Dayrom F., García‐Fernández, Rossana, Rivero, Maday Alonso del, Lamazares, Emilio, Pérez, Mariela, Varas, Laura A., Dı́az, Joaquı́n, Chávez, María Á., González-González, Yamile, Mansur, Manuel · 2011 · FEMS Yeast Research · DOI
Full title: Recombinant expression of ShPI-1A, a non-specific BPTI-Kunitz-type inhibitor, and its protection effect on proteolytic degradation of recombinant human miniproinsulin expressed in Pichia pastoris
Authors: Gil, Dayrom F., García‐Fernández, Rossana, Rivero, Maday Alonso del, Lamazares, Emilio, Pérez, Mariela, Varas, Laura A., Dı́az, Joaquı́n, Chávez, María Á., González-González, Yamile, Mansur, Manuel
PaperHeterologous protein expression in Pichia thermomethanolica BCC16875, a thermotolerant methylotrophic yeast and characterization of N-linked glycosylation in secreted protein.
Full title: Heterologous protein expression in Pichia thermomethanolica BCC16875, a thermotolerant methylotrophic yeast and characterization of N-linked glycosylation in secreted protein.
PaperSaposin B mobilizes lipids from cholesterol-poor and bis(monoacylglycero)phosphate-rich membranes at acidic pH. Unglycosylated patient variant saposin B lacks lipid-extraction capacity.
Remmel, Natascha, Locatelli-Hoops, Silvia, Breiden, Bernadette, Schwarzmann, Guenter, Sandhoff, Konrad · 2007 · The FEBS journal · DOI
Full title: Saposin B mobilizes lipids from cholesterol-poor and bis(monoacylglycero)phosphate-rich membranes at acidic pH. Unglycosylated patient variant saposin B lacks lipid-extraction capacity.
PaperIsolation of a laccase-coding gene from the lignin-degrading fungus<i>Phlebia brevispora</i>BAFC 633 and heterologous expression in<i>Pichia pastoris</i>
Fonseca, María Isabel, Molina, Melisa A., Winnik, D.L., Busi, María V., Fariña, Julia I., Villalba, Laura Lidia, Zapata, Pedro Darío · 2018 · Journal of Applied Microbiology · DOI
Full title: Isolation of a laccase-coding gene from the lignin-degrading fungus<i>Phlebia brevispora</i>BAFC 633 and heterologous expression in<i>Pichia pastoris</i>
Authors: Fonseca, María Isabel, Molina, Melisa A., Winnik, D.L., Busi, María V., Fariña, Julia I., Villalba, Laura Lidia, Zapata, Pedro Darío
PaperHigh level extracellular production of recombinant human interferon alpha 2b in glycoengineered Pichia pastoris: culture medium optimization, high cell density cultivation and biological characterization.
Katla, S, Karmakar, B, Tadi, S R R, Mohan, N, Anand, B, Pal, U, Sivaprakasam, S · 2019 · Journal of applied microbiology · DOI
Full title: High level extracellular production of recombinant human interferon alpha 2b in glycoengineered Pichia pastoris: culture medium optimization, high cell density cultivation and biological characterization.
Authors: Katla, S, Karmakar, B, Tadi, S R R, Mohan, N, Anand, B, Pal, U, Sivaprakasam, S
PaperGeneration and characterization of soluble interleukin-33 receptor fused with immunoglobulin gamma-1 constant domain expressed by <i>P</i> <i>ichia pastoris</i> yeast
Nguyen, Thi Thanh Thao, Truong, Thi Hoang Dieu, Le, Gia Bao, Duong, Hoa Xo, Bình, Nguyễn Quốc, Nguyen, Dang · 2015 · Journal of Pharmacy and Pharmacology · DOI
Full title: Generation and characterization of soluble interleukin-33 receptor fused with immunoglobulin gamma-1 constant domain expressed by <i>P</i> <i>ichia pastoris</i> yeast
Authors: Nguyen, Thi Thanh Thao, Truong, Thi Hoang Dieu, Le, Gia Bao, Duong, Hoa Xo, Bình, Nguyễn Quốc, Nguyen, Dang
PaperExpresión y producción de la lacasa POXA 1B recombinante de pleurotus ostreatus como alternativa para el tratamiento de los subproductos líquidos que se obtienen en el proceso de blanqueamiento de pulpa de celulosa
Full title: Expresión y producción de la lacasa POXA 1B recombinante de pleurotus ostreatus como alternativa para el tratamiento de los subproductos líquidos que se obtienen en el proceso de blanqueamiento de pulpa de celulosa
PaperExpression of a Bacillus phytase C gene in Pichia pastoris and properties of the recombinant enzyme.
Guerrero-Olazarán, Martha, Rodríguez-Blanco, Lilí, Carreon-Treviño, J Gerardo, Gallegos-López, Juan A, Viader-Salvadó, José M · 2010 · Applied and environmental microbiology · DOI
Full title: Expression of a Bacillus phytase C gene in Pichia pastoris and properties of the recombinant enzyme.
Authors: Guerrero-Olazarán, Martha, Rodríguez-Blanco, Lilí, Carreon-Treviño, J Gerardo, Gallegos-López, Juan A, Viader-Salvadó, José M
PaperInactivation of a GAL4-Like Transcription Factor Improves Cell Fitness and Product Yield in Glycoengineered Pichia pastoris Strains
B. Jiang, Rebecca Argyros, J. Bukowski, S. Nelson, Nathan Sharkey, Sehoon Kim, Victoria Copeland, R. Davidson, Ronghua Chen, J. Zhuang, N. Sethuraman, T. Stadheim · 2014 · Applied and Environmental Microbiology · DOI
Full title: Inactivation of a GAL4-Like Transcription Factor Improves Cell Fitness and Product Yield in Glycoengineered Pichia pastoris Strains
Authors: B. Jiang, Rebecca Argyros, J. Bukowski, S. Nelson, Nathan Sharkey, Sehoon Kim, Victoria Copeland, R. Davidson, Ronghua Chen, J. Zhuang, N. Sethuraman, T. Stadheim
PaperN-Glycosylation Improves the Pepsin Resistance of Histidine Acid Phosphatase Phytases by Enhancing Their Stability at Acidic pHs and Reducing Pepsin's Accessibility to Its Cleavage Sites.
Niu, Canfang, Luo, Huiying, Shi, Pengjun, Huang, Huoqing, Wang, Yaru, Yang, Peilong, Yao, Bin · 2016 · Applied and environmental microbiology · DOI
Full title: N-Glycosylation Improves the Pepsin Resistance of Histidine Acid Phosphatase Phytases by Enhancing Their Stability at Acidic pHs and Reducing Pepsin's Accessibility to Its Cleavage Sites.
PaperMolecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12
Norsyuhada Alias, Mu'adz Ahmad Mazian, A. Salleh, M. Basri, R. Rahman · 2014 · Enzyme Research · DOI
Full title: Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12
Authors: Norsyuhada Alias, Mu'adz Ahmad Mazian, A. Salleh, M. Basri, R. Rahman
PaperExpression and Characterization of<i>Geobacillus stearothermophilus</i>SR74 Recombinant<i>α</i>-Amylase in<i>Pichia pastoris</i>
Gandhi, S. R., Salleh, Abu Bakar, Rahman, Raja Noor Zaliha Raja Abd, Leow, Thean Chor, Oslan, Siti Nurbaya · 2015 · BioMed Research International · DOI
Full title: Expression and Characterization of<i>Geobacillus stearothermophilus</i>SR74 Recombinant<i>α</i>-Amylase in<i>Pichia pastoris</i>
Authors: Gandhi, S. R., Salleh, Abu Bakar, Rahman, Raja Noor Zaliha Raja Abd, Leow, Thean Chor, Oslan, Siti Nurbaya
PaperProduction of codon-optimized Factor C fragment from Tachypleus gigas in the Pichia pastoris GS115 expression system for endotoxin detection
Bachtiar, Zubaidi, Mustopa, Apon Zaenal, Astuti, Rika Indri, Fauziyah, Fauziyah, Fatimah, Fatimah, Rozirwan, Rozirwan, Wulandari, Tuah Nanda Merlia, Wijaya, Dina Permata, Agustriani, Fitri, Arwansyah, Arwansyah, Irawan, Herman, Mamangkey, Jendri · 2023 · Journal of Genetic Engineering and Biotechnology · DOI
Full title: Production of codon-optimized Factor C fragment from Tachypleus gigas in the Pichia pastoris GS115 expression system for endotoxin detection
PaperMolecular Cloning of cDNAs and Genes for Three α-Glucosidases from European Honeybees,<i>Apis mellifera</i>L., and Heterologous Production of Recombinant Enzymes in<i>Pichia pastoris</i>
Full title: Molecular Cloning of cDNAs and Genes for Three α-Glucosidases from European Honeybees,<i>Apis mellifera</i>L., and Heterologous Production of Recombinant Enzymes in<i>Pichia pastoris</i>
PaperLC-MS determination of glycosylation pattern on glycoproteins as critical quality attribute for biopharmaceuticals and potential markers for diseases
Full title: LC-MS determination of glycosylation pattern on glycoproteins as critical quality attribute for biopharmaceuticals and potential markers for diseases
PaperCloning, expression in Komagataella phaffii, and biochemical characterization of recombinant sequence variants of Pseudomonas sp. S9 GDSL-esterase
Wicka-Grochocka, Monika, Cieśliński, Hubert, Wanarska, Marta · 2021 · Acta Biochimica Polonica · DOI
Full title: Cloning, expression in Komagataella phaffii, and biochemical characterization of recombinant sequence variants of Pseudomonas sp. S9 GDSL-esterase
Authors: Wicka-Grochocka, Monika, Cieśliński, Hubert, Wanarska, Marta
PaperEvaluating Pulses and Modified Fed-batch Feeding of Methanol to Increase Expression Level of Human Insulin Precursor in Pichia Pastoris High-Density Cultivation
Putro, Eko Wahyu, Nurdiani, Dini, Hariyatun, Utami, Nuruliawaty, Kusharyoto, Wien, Juanssilfero, Ario Betha, Silvia, Rozy Ayu · 2022 · International Journal on Advanced Science Engineering and Information Technology · DOI
Full title: Evaluating Pulses and Modified Fed-batch Feeding of Methanol to Increase Expression Level of Human Insulin Precursor in Pichia Pastoris High-Density Cultivation
PaperDevelopment of Higher Molecular Weight of Recombinant Human Interferon Alpha-2a by Albumin Fusion Technology in Methilotropic Yeast Pichia Pastoris
Ningrum, Ratih Asmana, Herawati, Neng, Wardiana, Andri · 2017 · International Journal on Advanced Science Engineering and Information Technology · DOI
Full title: Development of Higher Molecular Weight of Recombinant Human Interferon Alpha-2a by Albumin Fusion Technology in Methilotropic Yeast Pichia Pastoris
PaperScalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris
Conacher, Cleo G., García-Aparicio, María P., Coetzee, Gerhardt, Zyl, Willem H. van, Görgens, Johann F. · 2019 · Research Square (Research Square) · DOI
Full title: Scalable methanol-free production of recombinant glucuronoyl esterase in Pichia pastoris
Authors: Conacher, Cleo G., García-Aparicio, María P., Coetzee, Gerhardt, Zyl, Willem H. van, Görgens, Johann F.
PaperExpression and Purification of Active Human Kinases Using Pichia pastoris as A General-Purpose Host
Aziz, May H. Abdel, Fan, Yao, Liu, Lijun, Moasser, Mark M., Fu, Haian, Jura, Natalia, Arkin, Michelle R. · 2019 · Research Square (Research Square) · DOI
Full title: Expression and Purification of Active Human Kinases Using Pichia pastoris as A General-Purpose Host
Authors: Aziz, May H. Abdel, Fan, Yao, Liu, Lijun, Moasser, Mark M., Fu, Haian, Jura, Natalia, Arkin, Michelle R.
PaperExpression and Purification of Active Human Kinases Using Pichia pastoris as A General-Purpose Host
Aziz, May H. Abdel, Fan, Yao, Liu, Lijun, Moasser, Mark M., Fu, Haian, Jura, Natalia, Arkin, Michelle R. · 2020 · Research Square (Research Square) · DOI
Full title: Expression and Purification of Active Human Kinases Using Pichia pastoris as A General-Purpose Host
Authors: Aziz, May H. Abdel, Fan, Yao, Liu, Lijun, Moasser, Mark M., Fu, Haian, Jura, Natalia, Arkin, Michelle R.
PaperMolecular Engineering Improves Antigen Quality and Enables Integrated Manufacturing of A Trivalent Subunit Vaccine Candidate for Rotavirus
Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawalijt, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher · 2020 · Research Square (Research Square) · DOI
Full title: Molecular Engineering Improves Antigen Quality and Enables Integrated Manufacturing of A Trivalent Subunit Vaccine Candidate for Rotavirus
Authors: Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawalijt, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher
PaperOptimization of Fermentation Conditions for Recombinant Lipase Expression At Small Scale Using Response Surface Methodology for Preliminary Production In Two Bioreactor Platforms.
López, Angela Liliana Meza, Acosta‐González, Alejandro, Manrique, Ingrid Yamile Pulido, Correa, Rosa Erlide Prieto, Junca, Carlos Jimenez · 2021 · Research Square (Research Square) · DOI
Full title: Optimization of Fermentation Conditions for Recombinant Lipase Expression At Small Scale Using Response Surface Methodology for Preliminary Production In Two Bioreactor Platforms.
Authors: López, Angela Liliana Meza, Acosta‐González, Alejandro, Manrique, Ingrid Yamile Pulido, Correa, Rosa Erlide Prieto, Junca, Carlos Jimenez
PaperHigh throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux
Fina, Albert, Millard, Pierre, Albiol, Joan, Ferrer, Pau, Heux, Stéphanie · 2023 · Research Square (Research Square) · DOI
Full title: High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux
PaperA Single-Vector CRISPR/Cas9 System for Genome Editing and Heterologous Enzyme Secretion in Saccharomyces cerevisiae: A Case Study on Pectate Lyase for Coffee Mucilage Removal
Lam, Linda, Nhi, Nguyen Huynh Ha, Lan, Vo Thi Hoang, Hau, Nguyen Van, Nguyen, Hieu · 2025 · DOI
Full title: A Single-Vector CRISPR/Cas9 System for Genome Editing and Heterologous Enzyme Secretion in Saccharomyces cerevisiae: A Case Study on Pectate Lyase for Coffee Mucilage Removal
Authors: Lam, Linda, Nhi, Nguyen Huynh Ha, Lan, Vo Thi Hoang, Hau, Nguyen Van, Nguyen, Hieu
PaperCharacterization of recombinant Bacillus halodurans CM1 xylanase produced by Pichia pastoris KM71 and its potential application in bleaching process of bagasse pulp
Haniyya, Haniyya, Mulyawati, Lina, Helianti, Is, Pinmanee, Phitsanu, Kocharin, Kanokarn, Cantasingh, Duriya, Nimchua, Thidarat · 2021 · Indonesian Journal of Biotechnology · DOI
Full title: Characterization of recombinant Bacillus halodurans CM1 xylanase produced by Pichia pastoris KM71 and its potential application in bleaching process of bagasse pulp
PaperExtracellular expression and characterization of an α-glucosidase from Oryza sativa and its transglycosylation for synthesis of 2-O-α-D-glucopyranosyl-L-ascorbic acid
Qi, Xuelian, Shao, Junlan, Cheng, Yinchu, He, Xiaoying, Li, Yan, Jia, Honghua, Yan, Ming · 2021 · DOI
Full title: Extracellular expression and characterization of an α-glucosidase from Oryza sativa and its transglycosylation for synthesis of 2-O-α-D-glucopyranosyl-L-ascorbic acid
PaperIdentifying bottleneck reactions and developing a systemic fed-batch feeding strategy of Pichia pastoris through fine-tuning of methanol utilization pathway
Boojari, Mohammad Amin, Ghaledari, Fatemeh Rajabi, Motamedian, Ehsan, Soleimani, Mehdi, Shojaosadati, Seyed Abbas · 2022 · DOI
Full title: Identifying bottleneck reactions and developing a systemic fed-batch feeding strategy of Pichia pastoris through fine-tuning of methanol utilization pathway
Authors: Boojari, Mohammad Amin, Ghaledari, Fatemeh Rajabi, Motamedian, Ehsan, Soleimani, Mehdi, Shojaosadati, Seyed Abbas
PaperUnderstanding the impact of bioprocess conditions on monoclonal antibody glycosylation in mammalian cell cultures through experimental and computational analyses
Sou, Si Nga · 2015 · Spiral (Imperial College London) · DOI
Full title: Understanding the impact of bioprocess conditions on monoclonal antibody glycosylation in mammalian cell cultures through experimental and computational analyses
PaperSafety of the proposed amendment of the specifications for steviol glycosides (E 960) as a food additive: Rebaudioside M produced via enzyme‐catalysed bioconversion of purified stevia leaf extract
Younes, Maged, Aquilina, Gabriele, Engel, Karl‐Heinz, Fowler, Paul, Fernández, María José Frutos, Fürst, Peter, Gürtler, Rainer, Gundert‐Remy, Ursula, Husøy, Trine, Mennes, Wim, Moldéus, Peter, Oskarsson, Agneta, Shah, Romina, Waalkens‐Berendsen, Ine, Wölfle, Detlef, Degen, Gisela H., Gott, David Michael, Leblanc, Jean‐Charles, Herman, Lieve, Aguilera, Jaime, Giarola, Alessandra, Rincón, Ana María, Castle, Laurence, Castle, Laurence · 2019 · EFSA Journal · DOI
Full title: Safety of the proposed amendment of the specifications for steviol glycosides (E 960) as a food additive: Rebaudioside M produced via enzyme‐catalysed bioconversion of purified stevia leaf extract
PaperA comparison of different constitutive expression systems for the production of bovine chymosin in Komagataella phaffii
Barros, Roberta F, Marco, Janice Lisboa De, Piva, Luíza Cesca, Coelho, Cíntia Marques, Moraes, Lídia Maria Pepe de, Torres, Fernando Araripe Gonçalves · 2025 · Journal of Dairy Science · DOI
Full title: A comparison of different constitutive expression systems for the production of bovine chymosin in Komagataella phaffii
Authors: Barros, Roberta F, Marco, Janice Lisboa De, Piva, Luíza Cesca, Coelho, Cíntia Marques, Moraes, Lídia Maria Pepe de, Torres, Fernando Araripe Gonçalves
PaperCation-exchange purification of mutagenized bovine beta-casein expressed in transgenic mouse milk: its putative Asn68-linked glycan is heterogeneous.
Choi, B K, Bleck, G T, Jimenez-Flores, R · 2001 · Journal of dairy science · DOI
Full title: Cation-exchange purification of mutagenized bovine beta-casein expressed in transgenic mouse milk: its putative Asn68-linked glycan is heterogeneous.
PaperStudy of glycerol-methanol and sorbitol-methanol mixed feed strategies for recombinant hPDGF-BB production in Pichia pastoris
Trần, Lạc Hà, Lê, Nghĩa Trung, Nguyen, Bao, Huỳnh, Vinh Hiền, Đặng, Huy Hoàng, Lê, Đông Đình, Nguyễn, Nhân Trí · 2021 · Science & Technology Development Journal - Engineering and Technology · DOI
Full title: Study of glycerol-methanol and sorbitol-methanol mixed feed strategies for recombinant hPDGF-BB production in Pichia pastoris
Authors: Trần, Lạc Hà, Lê, Nghĩa Trung, Nguyen, Bao, Huỳnh, Vinh Hiền, Đặng, Huy Hoàng, Lê, Đông Đình, Nguyễn, Nhân Trí
Year: 2021
Venue: Science & Technology Development Journal - Engineering and Technology
PaperImplementing Chemostat Fermentation of Pichia Pastoris Producing Recombinant HBsAg to Optimize Cell Density Affected by Methanol Rate
Rahimi, Alireza, Karimi, Afzal, Hosseini, Seyed Mohammad, Aghdasinia, Hassan, Mianroodi, Reza Arabi, Nieto-Taype, M, Garcia-Ortega, X, Albiol, J, Montesinos-Segu, J, Valero, F, Muoz, M, Rincn, J Juregui, Carren, L, Vela, N, Karbalaei, M, Rezaee, S, Farsiani, H, Rahimi, A, Hosseini, S, Javidanbardan, A, Khatami, M, Rahimi, A, Hosseini, S, Karimi, A, Aghdasinia, H, Mianroodi, R Arabi, Hosseini, S, Javidanbardan, A, Khatami, M, Tripathi, N, Shrivastava, A, Oak, A, Jansen, G, Chan, C, Cregg, J, Cereghino, J, Shi, J, Higgins, D, Cos, O, Ramn, R, Montesinos, J, Valero, F, James, A, Morvarid, A, Zeenathul, N, Tam, Y, Zuridah, H, Mohd-Azmi, M, Azizon, B, Gurramkonda, C, Adnan, A, Gbel, T, Lnsdorf, H, Ross, A, Nemani, S, Swaminathan, S, Khanna, N, Rinas, U, Cereghino, G, Cereghino, J, Ilgen, C, Cregg, J, Julien, C, Singh, A, Narang, A, Takagi, S, Tsutsumi, N, Terui, Y, Kong, X, Yurimoto, H, Sakai, Y, Hardy, E, Martnez, E, Diago, D, Daz, R, Gonzlez, D, Herrera, L, Hosseini, S, Sarvari, T, Bashiri, G, Khatami, M, Shojaosadati, S, Yazdani, R, Shams-Bakhsh, M, Hassani-Mehraban, A, Arab, S, Thelen, N, Thiry, M, Crommen, J, Fillet, M, Jacobs, N, Brans, A, Servais, A.-C, Tam, Y, Allaudin, Z, Lila, M, Bahaman, A, Tan, J, Rezaei, M, Chauhan, A · 2021 · Biointerface Research in Applied Chemistry · DOI
Full title: Implementing Chemostat Fermentation of Pichia Pastoris Producing Recombinant HBsAg to Optimize Cell Density Affected by Methanol Rate
Authors: Rahimi, Alireza, Karimi, Afzal, Hosseini, Seyed Mohammad, Aghdasinia, Hassan, Mianroodi, Reza Arabi, Nieto-Taype, M, Garcia-Ortega, X, Albiol, J, Montesinos-Segu, J, Valero, F, Muoz, M, Rincn, J Juregui, Carren, L, Vela, N, Karbalaei, M, Rezaee, S, Farsiani, H, Rahimi, A, Hosseini, S, Javidanbardan, A, Khatami, M, Rahimi, A, Hosseini, S, Karimi, A, Aghdasinia, H, Mianroodi, R Arabi, Hosseini, S, Javidanbardan, A, Khatami, M, Tripathi, N, Shrivastava, A, Oak, A, Jansen, G, Chan, C, Cregg, J, Cereghino, J, Shi, J, Higgins, D, Cos, O, Ramn, R, Montesinos, J, Valero, F, James, A, Morvarid, A, Zeenathul, N, Tam, Y, Zuridah, H, Mohd-Azmi, M, Azizon, B, Gurramkonda, C, Adnan, A, Gbel, T, Lnsdorf, H, Ross, A, Nemani, S, Swaminathan, S, Khanna, N, Rinas, U, Cereghino, G, Cereghino, J, Ilgen, C, Cregg, J, Julien, C, Singh, A, Narang, A, Takagi, S, Tsutsumi, N, Terui, Y, Kong, X, Yurimoto, H, Sakai, Y, Hardy, E, Martnez, E, Diago, D, Daz, R, Gonzlez, D, Herrera, L, Hosseini, S, Sarvari, T, Bashiri, G, Khatami, M, Shojaosadati, S, Yazdani, R, Shams-Bakhsh, M, Hassani-Mehraban, A, Arab, S, Thelen, N, Thiry, M, Crommen, J, Fillet, M, Jacobs, N, Brans, A, Servais, A.-C, Tam, Y, Allaudin, Z, Lila, M, Bahaman, A, Tan, J, Rezaei, M, Chauhan, A
ThesisInfluence of low dissolved oxygen concentration on incrasing the expression of recombinant rabies virus glycoprotein (RVGP) by Drosophila melanogaster S2 cells cultivated in a bioreactor
Full title: Influence of low dissolved oxygen concentration on incrasing the expression of recombinant rabies virus glycoprotein (RVGP) by Drosophila melanogaster S2 cells cultivated in a bioreactor
ThesisAvaliation of the induction time and metanol concentration in the expresión of L-asparaginase II from Saccharomyces cerevisiae using Pichia pastoris ('Mut POT. S')
Full title: Avaliation of the induction time and metanol concentration in the expresión of L-asparaginase II from Saccharomyces cerevisiae using Pichia pastoris ('Mut POT. S')
ThesisMetabolic engineering of glycosylation and secretion pathways in Pichia pastoris and its application in production of the human recombinant enzyme N-acetylgalactosamine-6-sulfate sulfatase
Rodríguez López, Edwin Alexander · 2018 · Theses · DOI
Full title: Metabolic engineering of glycosylation and secretion pathways in Pichia pastoris and its application in production of the human recombinant enzyme N-acetylgalactosamine-6-sulfate sulfatase
ThesisMolecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Dalvie, Neil C; Brady, Joseph R; Crowell, Laura E et al. · 2021 · Theses · DOI
Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Authors: Dalvie, Neil C; Brady, Joseph R; Crowell, Laura E; Tracey, Mary K; Biedermann, Andrew M; Kaur, Kawaljit; Hickey, John M; Kristensen, D. L; Bonnyman, Alexandra D; Rodriguez-Aponte, Sergio A; Whittaker, Charles A; Bok, Marina; Vega, Celina; Mukhopadhyay, Tarit K; Joshi, Sangeeta B; Volkin, David B
ThesisMinimal purification method enables developability assessment of recombinant proteins
Rodriguez‐Aponte, Sergio A; Naranjo, Christopher A; Johnston, Ryan S et al. · 2025 · Theses · DOI
Full title: Minimal purification method enables developability assessment of recombinant proteins
Authors: Rodriguez‐Aponte, Sergio A; Naranjo, Christopher A; Johnston, Ryan S; Dalvie, Neil C; Crowell, Laura E; Bajoria, Sakshi; Kumru, Ozan S; Joshi, Sangeeta B; Volkin, David B; Love, J Christopher
ThesisSpoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially therapeutic recombinant glycoprotein factor H
Full title: Spoonful of sugar helps the medicine go down: biomanufacture in glycoengineered Pichia pastoris of the potentially therapeutic recombinant glycoprotein factor H
ThesisCaractérisation quantitative de la croissance et du système d’expression d’une souche recombinante de Pichia pastoris Mut+: influence du taux de dilution et du type de substrat
Full title: Caractérisation quantitative de la croissance et du système d’expression d’une souche recombinante de Pichia pastoris Mut+: influence du taux de dilution et du type de substrat
Full title: Optimisation de la production d'avidine sécrétée par une lignée mut+ de P. pastoris en culture fed-batch à une haute densité cellulaire
ThesisMixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures
Jungo, Carmen; Marison, Ian; von Stockar, Urs · 2007 · Theses · DOI
Full title: Mixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures
Authors: Jungo, Carmen; Marison, Ian; von Stockar, Urs
ThesisA quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris
Jungo, Carmen; Schenk, Jonas; Pasquier, Muriel et al. · 2007 · Theses · DOI
Full title: A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris
ThesisShaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000L by surface aeration with air
Zhang, X.; Stettler, M.; Reif, O. et al. · 2009 · École polytechnique fédérale de Lausanne (EPFL) · DOI
Full title: Shaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000L by surface aeration with air
Authors: Zhang, X.; Stettler, M.; Reif, O.; Kocourek, A.; Dejesus, M.; Hacker, D.; Wurm, F.
Year: 2009
Venue: École polytechnique fédérale de Lausanne (EPFL)
ThesisDevelopment of a predictive model for cell growth in shaken bioreactors as a function of the effects of temperature, oxygen transfer rate and seeding cell concentration
Full title: Development of a predictive model for cell growth in shaken bioreactors as a function of the effects of temperature, oxygen transfer rate and seeding cell concentration
ThesisInfluence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut(+) strain
Schenk, Jonas; Balazs, Krisztina; Jungo, Carmen et al. · 2010 · Theses · DOI
Full title: Influence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut(+) strain
Authors: Schenk, Jonas; Balazs, Krisztina; Jungo, Carmen; Urfer, Julien; Wegmann, Carole; Zocchi, Andrea; Marison, Ian W.; von Stockar, Urs
ThesisA comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation
Monteil, Dominique T.; Juvet, Valentin; Paz, Jonathan et al. · 2017 · Theses · DOI
Full title: A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation
Authors: Monteil, Dominique T.; Juvet, Valentin; Paz, Jonathan; Moniatte, Marc; Baldi, Lucia; Hacker, David L.; Wurm, Florian M.
ThesisInnovative methods of knowledge-driven methanol feedback control and state estimation for recombinant Pichia pastoris fed-batch cultivation
Brunner, V., Metzenmacher, M., Birle, S., Geier, D., Becker, T. · 2017 · Theses · DOI
Full title: Innovative methods of knowledge-driven methanol feedback control and state estimation for recombinant Pichia pastoris fed-batch cultivation
Authors: Brunner, V., Metzenmacher, M., Birle, S., Geier, D., Becker, T.
ThesisOptimising the biosynthesis of oxygenated and acetylated Taxol precursors in Saccharomyces cerevisiae using advanced bioprocessing strategies
Walls, Laura E.; Malci, Koray; Nowrouzi, Behnaz et al. · 2021 · Theses · DOI
Full title: Optimising the biosynthesis of oxygenated and acetylated Taxol precursors in Saccharomyces cerevisiae using advanced bioprocessing strategies
Authors: Walls, Laura E.; Malci, Koray; Nowrouzi, Behnaz; Li, Rachel; d'Espaux, Leo; Wong, Jeff; Dennis, Jonathan A.; Semiao, Andrea J C; Wallace, Stephen; Martinez, José L.; id_orcid 0000-0002-4490-8534; Keasling, Jay D.; Rios-Solis, Leonardo
ThesisFluctuations in glucose availability prevent global proteome changes and physiological transition during prolonged chemostat cultivations of S. cerevisiae
Wright, Naia Risager; Wulff, Tune; Palmqvist, Eva Akke et al. · 2020 · Technical University of Denmark (DTU) · DOI
Full title: Fluctuations in glucose availability prevent global proteome changes and physiological transition during prolonged chemostat cultivations of S. cerevisiae
Authors: Wright, Naia Risager; Wulff, Tune; Palmqvist, Eva Akke; Jørgensen, Thomas Rubæk; Workman, Christopher T.; id_orcid 0000-0002-2210-3743; Sonnenschein, Nikolaus; id_orcid 0000-0002-7581-4936; Rønnest, Nanna Petersen; Herrgård, Markus J.
ThesisBurden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan et al. · 2022 · Theses · DOI
Full title: Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Authors: Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan; Jensen, Michael Krogh; id_orcid 0000-0001-7574-4707; Workman, Christopher T.; id_orcid 0000-0002-2210-3743
ThesisAutomated Electrochemical Glucose Biosensor Platform as an Efficient Tool Toward On-Line Fermentation Monitoring:Novel Application Approaches and Insights
Pontius, Katrin; Semenova, Daria; Silina, Yuliya E. et al. · 2020 · Theses · DOI
Full title: Automated Electrochemical Glucose Biosensor Platform as an Efficient Tool Toward On-Line Fermentation Monitoring:Novel Application Approaches and Insights
ThesisEngineering ergothioneine production in <i>Yarrowia lipolytica</i>
van der Hoek, Steven A.; Rusnák, Matej; Jacobsen, Irene Hjorth et al. · 2022 · Theses · DOI
Full title: Engineering ergothioneine production in <i>Yarrowia lipolytica</i>
Authors: van der Hoek, Steven A.; Rusnák, Matej; Jacobsen, Irene Hjorth; Martínez, José L.; id_orcid 0000-0002-4490-8534; Kell, Douglas B.; Borodina, Irina
ThesisIntegrated Optimization of Hemoprotein Biomanufacturing in Yeast:From Upstream Engineering of Heme Synthesis to Downstream Purification of Human Hemoglobin
Full title: Integrated Optimization of Hemoprotein Biomanufacturing in Yeast:From Upstream Engineering of Heme Synthesis to Downstream Purification of Human Hemoglobin
ThesisDynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica
Kerssemakers, Abraham Antonius Johannes; id_orcid 0000-0002-3462-4642; Øzmerih, Süleyman et al. · 2023 · Theses · DOI
Full title: Dynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica
Authors: Kerssemakers, Abraham Antonius Johannes; id_orcid 0000-0002-3462-4642; Øzmerih, Süleyman; Sin, Gürkan; id_orcid 0000-0003-0513-4502; Sudarsan, Suresh
ThesisEmergence of Phenotypically Distinct Subpopulations Is a Factor in Adaptation of Recombinant <i>Saccharomyces cerevisiae</i> under Glucose-Limited Conditions
Wright, Naia Risager; Jessop-Fabre, Mathew M.; id_orcid 0000-0001-7534-2230 et al. · 2022 · Theses · DOI
Full title: Emergence of Phenotypically Distinct Subpopulations Is a Factor in Adaptation of Recombinant <i>Saccharomyces cerevisiae</i> under Glucose-Limited Conditions
Authors: Wright, Naia Risager; Jessop-Fabre, Mathew M.; id_orcid 0000-0001-7534-2230; Sánchez, Benjamin J.; Wulff, Tune; Workman, Christopher T.; id_orcid 0000-0002-2210-3743; Rønnest, Nanna Petersen; Sonnenschein, Nikolaus; id_orcid 0000-0002-7581-4936
ThesisUnderstanding gradients in industrial bioreactors
Nadal-Rey, Gisela; McClure, Dale D.; Kavanagh, John M. et al. · 2021 · Technical University of Denmark (DTU) · DOI
Full title: Understanding gradients in industrial bioreactors
Authors: Nadal-Rey, Gisela; McClure, Dale D.; Kavanagh, John M.; Cornelissen, Sjef; Fletcher, David F.; Gernaey, Krist V.; id_orcid 0000-0002-0364-1773
ThesisImproving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions
Hansen, Henning Gram; Pristovsek, Nusa; Kildegaard, Helene Faustrup et al. · 2017 · Theses · DOI
Full title: Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions
Authors: Hansen, Henning Gram; Pristovsek, Nusa; Kildegaard, Helene Faustrup; Min Lee, Gyun
ThesisOptimising the biosynthesis of oxygenated and acetylated Taxol precursors in Saccharomyces cerevisiae using advanced bioprocessing strategies
Walls, Laura E.; Malci, Koray; Nowrouzi, Behnaz et al. · 2021 · Theses · DOI
Full title: Optimising the biosynthesis of oxygenated and acetylated Taxol precursors in Saccharomyces cerevisiae using advanced bioprocessing strategies
Authors: Walls, Laura E.; Malci, Koray; Nowrouzi, Behnaz; Li, Rachel; d'Espaux, Leo; Wong, Jeff; Dennis, Jonathan A.; Semiao, Andrea J C; Wallace, Stephen; Martinez, José L.; id_orcid 0000-0002-4490-8534; Keasling, Jay D.; Rios-Solis, Leonardo
ThesisFluctuations in glucose availability prevent global proteome changes and physiological transition during prolonged chemostat cultivations of S. cerevisiae
Wright, Naia Risager; Wulff, Tune; Palmqvist, Eva Akke et al. · 2020 · Technical University of Denmark (DTU) · DOI
Full title: Fluctuations in glucose availability prevent global proteome changes and physiological transition during prolonged chemostat cultivations of S. cerevisiae
Authors: Wright, Naia Risager; Wulff, Tune; Palmqvist, Eva Akke; Jørgensen, Thomas Rubæk; Workman, Christopher T.; id_orcid 0000-0002-2210-3743; Sonnenschein, Nikolaus; id_orcid 0000-0002-7581-4936; Rønnest, Nanna Petersen; Herrgård, Markus J.
ThesisBurden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan et al. · 2022 · Theses · DOI
Full title: Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Authors: Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan; Jensen, Michael Krogh; id_orcid 0000-0001-7574-4707; Workman, Christopher T.; id_orcid 0000-0002-2210-3743
ThesisAutomated Electrochemical Glucose Biosensor Platform as an Efficient Tool Toward On-Line Fermentation Monitoring:Novel Application Approaches and Insights
Pontius, Katrin; Semenova, Daria; Silina, Yuliya E. et al. · 2020 · Theses · DOI
Full title: Automated Electrochemical Glucose Biosensor Platform as an Efficient Tool Toward On-Line Fermentation Monitoring:Novel Application Approaches and Insights
ThesisUsing titer and titer normalized to confluence are complementary strategies for obtaining Chinese hamster ovary cell lines with high volumetric productivity of etanercept
Pristovšek, Nuša; Hansen, Henning Gram; Sergeeva, Daria et al. · 2018 · Theses · DOI
Full title: Using titer and titer normalized to confluence are complementary strategies for obtaining Chinese hamster ovary cell lines with high volumetric productivity of etanercept
Authors: Pristovšek, Nuša; Hansen, Henning Gram; Sergeeva, Daria; Borth, Nicole; Min Lee, Gyun; Andersen, Mikael Rørdam; id_orcid 0000-0003-4794-6808; Kildegaard, Helene Faustrup
ThesisEngineering ergothioneine production in <i>Yarrowia lipolytica</i>
van der Hoek, Steven A.; Rusnák, Matej; Jacobsen, Irene Hjorth et al. · 2022 · Theses · DOI
Full title: Engineering ergothioneine production in <i>Yarrowia lipolytica</i>
Authors: van der Hoek, Steven A.; Rusnák, Matej; Jacobsen, Irene Hjorth; Martínez, José L.; id_orcid 0000-0002-4490-8534; Kell, Douglas B.; Borodina, Irina
ThesisIntegrated Optimization of Hemoprotein Biomanufacturing in Yeast:From Upstream Engineering of Heme Synthesis to Downstream Purification of Human Hemoglobin
Full title: Integrated Optimization of Hemoprotein Biomanufacturing in Yeast:From Upstream Engineering of Heme Synthesis to Downstream Purification of Human Hemoglobin
ThesisReal-time direct cell concentration and viability determination using a fully automated microfluidic platform for standalone process monitoring
Rodrigues de Sousa Nunes, Pedro André; Kjaerulff, S.; Dufva, Martin et al. · 2015 · Technical University of Denmark (DTU) · DOI
Full title: Real-time direct cell concentration and viability determination using a fully automated microfluidic platform for standalone process monitoring
Authors: Rodrigues de Sousa Nunes, Pedro André; Kjaerulff, S.; Dufva, Martin; id_orcid 0000-0001-5449-0189; Mogensen, Klaus Bo
ThesisDynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica
Kerssemakers, Abraham Antonius Johannes; id_orcid 0000-0002-3462-4642; Øzmerih, Süleyman et al. · 2023 · Theses · DOI
Full title: Dynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica
Authors: Kerssemakers, Abraham Antonius Johannes; id_orcid 0000-0002-3462-4642; Øzmerih, Süleyman; Sin, Gürkan; id_orcid 0000-0003-0513-4502; Sudarsan, Suresh
ThesisKnockout of sialidase and pro-apoptotic genes in Chinese hamster ovary cells enables the production of recombinant human erythropoietin in fed-batch cultures
Ha, Tae Kwang; id_orcid 0000-0001-7804-0051; Hansen, Anders Holmgaard et al. · 2020 · Theses · DOI
Full title: Knockout of sialidase and pro-apoptotic genes in Chinese hamster ovary cells enables the production of recombinant human erythropoietin in fed-batch cultures
Authors: Ha, Tae Kwang; id_orcid 0000-0001-7804-0051; Hansen, Anders Holmgaard; id_orcid 0000-0002-6007-4787; Kildegaard, Helene Faustrup; Lee, Gyun Min
ThesisEmergence of Phenotypically Distinct Subpopulations Is a Factor in Adaptation of Recombinant <i>Saccharomyces cerevisiae</i> under Glucose-Limited Conditions
Wright, Naia Risager; Jessop-Fabre, Mathew M.; id_orcid 0000-0001-7534-2230 et al. · 2022 · Theses · DOI
Full title: Emergence of Phenotypically Distinct Subpopulations Is a Factor in Adaptation of Recombinant <i>Saccharomyces cerevisiae</i> under Glucose-Limited Conditions
Authors: Wright, Naia Risager; Jessop-Fabre, Mathew M.; id_orcid 0000-0001-7534-2230; Sánchez, Benjamin J.; Wulff, Tune; Workman, Christopher T.; id_orcid 0000-0002-2210-3743; Rønnest, Nanna Petersen; Sonnenschein, Nikolaus; id_orcid 0000-0002-7581-4936
ThesisUnderstanding gradients in industrial bioreactors
Nadal-Rey, Gisela; McClure, Dale D.; Kavanagh, John M. et al. · 2021 · Technical University of Denmark (DTU) · DOI
Full title: Understanding gradients in industrial bioreactors
Authors: Nadal-Rey, Gisela; McClure, Dale D.; Kavanagh, John M.; Cornelissen, Sjef; Fletcher, David F.; Gernaey, Krist V.; id_orcid 0000-0002-0364-1773
ThesisImproving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions
Hansen, Henning Gram; Pristovsek, Nusa; Kildegaard, Helene Faustrup et al. · 2017 · Theses · DOI
Full title: Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions
Authors: Hansen, Henning Gram; Pristovsek, Nusa; Kildegaard, Helene Faustrup; Min Lee, Gyun
ThesisExploration of the potential of nonconventional yeasts as cell factories by physiological characterisation and development of new expression tools
Full title: Exploration of the potential of nonconventional yeasts as cell factories by physiological characterisation and development of new expression tools
ThesisBiopharmaceuticals from microorganisms: from production to purification
Jozala, Angela Faustino; Geraldes, Danilo Costa; Tundisi, Louise Lacalendola et al. · 2018 · Theses · DOI
Full title: Biopharmaceuticals from microorganisms: from production to purification
Authors: Jozala, Angela Faustino; Geraldes, Danilo Costa; Tundisi, Louise Lacalendola; Feitosa, Valker de Araújo; Breyer, Carlos Alexandre [UNESP]; Cardoso, Samuel Leite; Mazzola, Priscila Gava; de Oliveira-Nascimento, Laura; Rangel-Yagui, Carlota de Oliveira; Magalhães, Pérola de Oliveira; de Oliveira, Marcos Antonio [UNESP]; Pessoa, Adalberto
ThesisOxygen transfer and gas holdup in airlift bioreactors assembled with helical flow promoters
Picão, Bruno W. [UNESP]; Gonçalves, Daniele O. [UNESP]; Ribeiro, Renata M. M. G. P. et al. · 2023 · Universidade Estadual Paulista (UNESP) · DOI
Full title: Oxygen transfer and gas holdup in airlift bioreactors assembled with helical flow promoters
Authors: Picão, Bruno W. [UNESP]; Gonçalves, Daniele O. [UNESP]; Ribeiro, Renata M. M. G. P.; Esperança, Mateus N.; Peixoto, Guilherme [UNESP]; Cerri, Marcel O. [UNESP]
ThesisCharacterization of Oxygen Transfer Conditions and their Effects on Phaffia Rhodozyma Growth and Carotenoid Production in Shake-flask Cultures
Liu, Yuanshuai; Wu, Jian-Yong; Ho, Kwok Ping · 2006 · Hong Kong University of Science and Technology (HKUST) · DOI
Full title: Characterization of Oxygen Transfer Conditions and their Effects on Phaffia Rhodozyma Growth and Carotenoid Production in Shake-flask Cultures
ThesisModeling of Xanthophyllomyces dendrorhous Growth on Glucose and Overflow Metabolism in Batch and Fed-Batch Cultures for Astaxanthin Production
Liu, Yuanshuai; Wu, Jian-Yong · 2008 · Theses · DOI
Full title: Modeling of Xanthophyllomyces dendrorhous Growth on Glucose and Overflow Metabolism in Batch and Fed-Batch Cultures for Astaxanthin Production
ThesisDevelopment of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition (SCADA) Software for Automation of High Cell Density Pichia pastoris Fermentations in a 30L Bioreactor
Full title: Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition (SCADA) Software for Automation of High Cell Density Pichia pastoris Fermentations in a 30L Bioreactor
ThesisTranslational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
Edwards-Jones, B; Aw, R; Barton, GR et al. · 2015 · Theses · DOI
Full title: Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
ThesisProduction and purification of an active CRM197 in Pichia pastoris and its immunological characterization using a Vi-typhoid antigen vaccine
Aw, Rochelle; Ashik, Muktadir R; Islam, Ariful AZM et al. · 2022 · Theses · DOI
Full title: Production and purification of an active CRM197 in Pichia pastoris and its immunological characterization using a Vi-typhoid antigen vaccine
Authors: Aw, Rochelle; Ashik, Muktadir R; Islam, Ariful AZM; Khan, Imran; Mainuddin, Mohammad; Islam, Md Ashraful; Ahasan, Mohammad M; Polizzi, Karen M
ThesisAccelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform
Sawatzki, Annina; Hans, Sebastian; Narayanan, Harini et al. · 2018 · Theses · DOI
Full title: Accelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform
ThesisEstratègies d'operació en el procés de producció de proteïnes heteròlogues en Pichia pastoris: aplicació de tècniques de monitorització i control
Full title: Estratègies d'operació en el procés de producció de proteïnes heteròlogues en Pichia pastoris: aplicació de tècniques de monitorització i control
ThesisMillora en el procés de producció d'una lipasa de Rhizopus oryzae en Pichia pastoris mitjançant tècniques de monitoratge i estratègies de cultiu alternatives
Full title: Millora en el procés de producció d'una lipasa de Rhizopus oryzae en Pichia pastoris mitjançant tècniques de monitoratge i estratègies de cultiu alternatives
ThesisProviding growth-decoupled strategies for recombinant protein production in Komagataella phaffii: from promoter identification to bioprocess scale-up
Full title: Providing growth-decoupled strategies for recombinant protein production in Komagataella phaffii: from promoter identification to bioprocess scale-up
Statistical Improvement of Batch Culture with Immobilized <i>Pichia pastoris</i> Cells for rPOXA 1B Laccase Production
Gouzy-Olmos, Michelle, Ch�ves-Tequia, Luis M., Rojas-Fajardo, María F., Morales-Álvarez, Edwin D., Rivera-Hoyos, Claudia M., Poutou‐Piñales, Raúl A., González-Neira, Eliana María, Reyes-Montaño, Edgar Antonio, Cardozo-Bernal, Ángela M., Gómez-Méndez, Luis David, Pedroza-Rodríguez, Aura M. · 2018
Full title: Statistical Improvement of Batch Culture with Immobilized <i>Pichia pastoris</i> Cells for rPOXA 1B Laccase Production
Authors: Gouzy-Olmos, Michelle, Ch�ves-Tequia, Luis M., Rojas-Fajardo, María F., Morales-Álvarez, Edwin D., Rivera-Hoyos, Claudia M., Poutou‐Piñales, Raúl A., González-Neira, Eliana María, Reyes-Montaño, Edgar Antonio, Cardozo-Bernal, Ángela M., Gómez-Méndez, Luis David, Pedroza-Rodríguez, Aura M.
Preparation and functional characterization of human vascular endothelial growth factor-melittin fusion protein with analysis of the antitumor activity in vitro and in vivo
Full title: Preparation and functional characterization of human vascular endothelial growth factor-melittin fusion protein with analysis of the antitumor activity in vitro and in vivo
Secretion and expression dynamics of a GFP-tagged mucin-type fusion protein in high cell density &lt;i&gt;Pichia pastoris&lt;/i&gt; bioreactor cultivations
Full title: Secretion and expression dynamics of a GFP-tagged mucin-type fusion protein in high cell density &lt;i&gt;Pichia pastoris&lt;/i&gt; bioreactor cultivations
High-level secretory expression, purification, and characterization of an anti-human Her II monoclonal antibody, trastuzumab, in the methylotrophic yeast &lt;i&gt;Pichia pastoris&lt;/i&gt;
Shibui, Tatsuro, Bando, Keisuke, Misawa, S. · 2013
Full title: High-level secretory expression, purification, and characterization of an anti-human Her II monoclonal antibody, trastuzumab, in the methylotrophic yeast &lt;i&gt;Pichia pastoris&lt;/i&gt;
Authors: Shibui, Tatsuro, Bando, Keisuke, Misawa, S.
Full title: Intensification of Recombinant Laccase Production from Sago Bioethanol Liquid Waste and Evaluation of The Enzyme for Synthetic Dye Decolourisation
Talaromyces marneffei Cu, Zn Superoxide Dismutase Recombinant Protein Expression in Pichia pastoris, Enzymatic Activity and Its Resistance to Oxidative Stress
S. Khanthawong, K. Wongsawan, R. Pratanaphon, N. Vanittanakom · 2024
Full title: Talaromyces marneffei Cu, Zn Superoxide Dismutase Recombinant Protein Expression in Pichia pastoris, Enzymatic Activity and Its Resistance to Oxidative Stress
Authors: S. Khanthawong, K. Wongsawan, R. Pratanaphon, N. Vanittanakom
Peer Review #1 of "Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli (v0.1)"
Full title: Peer Review #1 of "Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli (v0.1)"
Expression of human serum albumin in Pichia pastoris protease-deficient host and conjugation with gadolinium-diethylenetriamine pentaacetate for application as a contrast agent
Gaffar, Shabarni, Anggraeni, Rina, Novianti, Mia Tria, Ishmayana, Safri, Soedjanaatmadja, Ukun M.S. · 2021
Full title: Expression of human serum albumin in Pichia pastoris protease-deficient host and conjugation with gadolinium-diethylenetriamine pentaacetate for application as a contrast agent
Authors: Gaffar, Shabarni, Anggraeni, Rina, Novianti, Mia Tria, Ishmayana, Safri, Soedjanaatmadja, Ukun M.S.
Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfolded proteins using chemostat cultures
Hesketh, Andrew, Castrillo, Juan I., Sawyer, Trevor, Archer, David B., Oliver, Stephen G. · 2013
Full title: Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfolded proteins using chemostat cultures
Authors: Hesketh, Andrew, Castrillo, Juan I., Sawyer, Trevor, Archer, David B., Oliver, Stephen G.
Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter
Full title: Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter
High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors
Jankowski, Nina, Koschorreck, Katja, Urlacher, Vlada B. · 2020
Full title: High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors
Authors: Jankowski, Nina, Koschorreck, Katja, Urlacher, Vlada B.
Impact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-state-maintained continuous cultures
Korpys-Woźniak, Paulina, Kubiak, Piotr, Białas, W., Celińska, Ewelina · 2020
Full title: Impact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-state-maintained continuous cultures
Authors: Korpys-Woźniak, Paulina, Kubiak, Piotr, Białas, W., Celińska, Ewelina
Full title: Hyperosmolarity adversely impacts recombinant protein synthesis by Yarrowia lipolytica—molecular background revealed by quantitative proteomics
High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
Blanchard, Véronique, Gadkari, Rupali A., George, A. V. E., Roy, Satarupa Sinha, Gerwig, Gerrit J., Leeflang, Bas R., Dighe, Rajan R., Boelens, Rolf, Kamerling, Johannis P. · 2008
Full title: High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
Authors: Blanchard, Véronique, Gadkari, Rupali A., George, A. V. E., Roy, Satarupa Sinha, Gerwig, Gerrit J., Leeflang, Bas R., Dighe, Rajan R., Boelens, Rolf, Kamerling, Johannis P.
Full title: Cloning and expression of Saccharomyces cerevisiae SUC2 gene in yeast platform and characterization of recombinant enzyme biochemical properties.
Methanol addition after glucose depletion improves rPOXA 1B production under the pGap in P. pastoris X33: breaking the habit
Ardila-Leal, Leidy D., Poutou‐Piñales, Raúl A., Morales-Álvarez, Edwin D., Rivera-Hoyos, Claudia M., Pedroza-Rodríguez, Aura M., Quevedo-Hidalgo, Balkys, Pérez, Alejandro · 2021
Full title: Methanol addition after glucose depletion improves rPOXA 1B production under the pGap in P. pastoris X33: breaking the habit
Authors: Ardila-Leal, Leidy D., Poutou‐Piñales, Raúl A., Morales-Álvarez, Edwin D., Rivera-Hoyos, Claudia M., Pedroza-Rodríguez, Aura M., Quevedo-Hidalgo, Balkys, Pérez, Alejandro
Full title: Functional characterisation of a recombinant xylanase from<i>Pichia pastoris</i>and effect of the enzyme on nutrient digestibility in weaned pigs
High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin
Chen, Ximing, Li, Juan, Sun, Haili, Li, Shi-Weng, Chen, Tuo, Liu, Guangxiu, Dyson, Paul · 2017
Full title: High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin
Authors: Chen, Ximing, Li, Juan, Sun, Haili, Li, Shi-Weng, Chen, Tuo, Liu, Guangxiu, Dyson, Paul
Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Yu, Xiao‐Wei, Sun, Weihong, Wang, Ying-Zheng, Xu, Yan · 2017
Full title: Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Authors: Yu, Xiao‐Wei, Sun, Weihong, Wang, Ying-Zheng, Xu, Yan
Multi-omics integrative analysis with genome-scale metabolic model simulation reveals global cellular adaptation of Aspergillus niger under industrial enzyme production condition
Full title: Multi-omics integrative analysis with genome-scale metabolic model simulation reveals global cellular adaptation of Aspergillus niger under industrial enzyme production condition
Enzymatic activity of a recombinant β-1,4-endoglucanase from the Cotton Boll Weevil (Anthonomus grandis) aiming second generation ethanol production
Ibarra, Liz Nathalia, Alves, Ana Elizabeth Oliveira de Araújo, Antonino, José Dijair, Prado, Guilherme Souza, Pinto, Clidia Eduarda Moreira, Soccol, Carlos Ricardo, Vasconcelos, Érico Augusto Rosas de, Grossi‐de‐Sá, Maria Fátima · 2019
Full title: Enzymatic activity of a recombinant β-1,4-endoglucanase from the Cotton Boll Weevil (Anthonomus grandis) aiming second generation ethanol production
Authors: Ibarra, Liz Nathalia, Alves, Ana Elizabeth Oliveira de Araújo, Antonino, José Dijair, Prado, Guilherme Souza, Pinto, Clidia Eduarda Moreira, Soccol, Carlos Ricardo, Vasconcelos, Érico Augusto Rosas de, Grossi‐de‐Sá, Maria Fátima
Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden
Wollborn, David, Munkler, Lara Pauline, Horstmann, Rebekka, Germer, Andrea, Blank, Lars M., Büchs, Jochen · 2022
Full title: Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden
Authors: Wollborn, David, Munkler, Lara Pauline, Horstmann, Rebekka, Germer, Andrea, Blank, Lars M., Büchs, Jochen
Process development for an effective COVID-19 vaccine candidate harboring recombinant SARS-CoV-2 delta plus receptor binding domain produced by Pichia pastoris
Full title: Process development for an effective COVID-19 vaccine candidate harboring recombinant SARS-CoV-2 delta plus receptor binding domain produced by Pichia pastoris
Pathway elucidation of bioactive rhamnosylated ginsenosides in Panax ginseng and their de novo high-level production by engineered Saccharomyces cerevisiae
Full title: Pathway elucidation of bioactive rhamnosylated ginsenosides in Panax ginseng and their de novo high-level production by engineered Saccharomyces cerevisiae
Full title: Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification.
Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement
Full title: Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement
High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation
Full title: High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation
Full title: Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris
Metabolic flux analysis: a comprehensive review on sample preparation, analytical techniques, data analysis, computational modelling, and main application areas
Falco, Bruna de, Giannino, Francesco, Cartenì, Fabrizio, Mazzoleni, Stefano, Kim, Dong‐Hyun · 2022
Full title: Metabolic flux analysis: a comprehensive review on sample preparation, analytical techniques, data analysis, computational modelling, and main application areas
Authors: Falco, Bruna de, Giannino, Francesco, Cartenì, Fabrizio, Mazzoleni, Stefano, Kim, Dong‐Hyun
Biochemical, structural insights of newly isolated AA16 family of Lytic Polysaccharide Monooxygenase (LPMO) from <i>Aspergillus fumigatus</i> and investigation of its synergistic effect using biomass
Full title: Biochemical, structural insights of newly isolated AA16 family of Lytic Polysaccharide Monooxygenase (LPMO) from <i>Aspergillus fumigatus</i> and investigation of its synergistic effect using biomass
Structural and Functional Comparison of SARS-CoV-2-Spike Receptor Binding Domain Produced in <i>Pichia pastoris</i> and Mammalian Cells
Arbeitman, Claudia R., Auge, Gabriela, Blaustein, Matı́as, Bredeston, Luis M., Corapi, Enrique, Craig, Patricio O., Cossio, Leandro A., Daín, Liliana, D’Alessio, Cecilia, Elias, Fernanda, Fernández, Natalia, Gasulla, Javier, Gorojovsky, Natalia, Gudesblat, Gustavo E., Herrera, María Georgina, Ibañez, Lorena I., Idrovo, Tommy, Randon, Matías Iglesias, Kamenetzky, Laura, Nadra, Alejandro D., Noseda, Diego G., Paván, Carlos H., Pavan, María Florencia, Pignataro, María F., Román, Ernesto A., Ruberto, Lucas, Rubinstein, Natalia, Santos, Javier, Duarte, Francisco Velázquez, Zelada, Alicia M. · 2020
Full title: Structural and Functional Comparison of SARS-CoV-2-Spike Receptor Binding Domain Produced in <i>Pichia pastoris</i> and Mammalian Cells
Authors: Arbeitman, Claudia R., Auge, Gabriela, Blaustein, Matı́as, Bredeston, Luis M., Corapi, Enrique, Craig, Patricio O., Cossio, Leandro A., Daín, Liliana, D’Alessio, Cecilia, Elias, Fernanda, Fernández, Natalia, Gasulla, Javier, Gorojovsky, Natalia, Gudesblat, Gustavo E., Herrera, María Georgina, Ibañez, Lorena I., Idrovo, Tommy, Randon, Matías Iglesias, Kamenetzky, Laura, Nadra, Alejandro D., Noseda, Diego G., Paván, Carlos H., Pavan, María Florencia, Pignataro, María F., Román, Ernesto A., Ruberto, Lucas, Rubinstein, Natalia, Santos, Javier, Duarte, Francisco Velázquez, Zelada, Alicia M.
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher · 2020
Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Authors: Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher
Improving the heterologous production of fungal peroxygenases through an episomal <i>Pichia pastoris</i> promoter and signal peptide shuffling system
Püllmann, Pascal, Weissenborn, Martin J. · 2020
Full title: Improving the heterologous production of fungal peroxygenases through an episomal <i>Pichia pastoris</i> promoter and signal peptide shuffling system
Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters
Dalvie, Neil C., Rodriguez‐Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Célia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dong Soo, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanné, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith M., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice, Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher · 2021
Full title: Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters
Authors: Dalvie, Neil C., Rodriguez‐Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Célia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dong Soo, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanné, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith M., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice, Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher
Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered <i>Komagataella phaffii</i>
Dalvie, Neil C., Biedermann, Andrew M., Rodriguez‐Aponte, Sergio A., Naranjo, Christopher A., Rao, Harish, Rajurkar, Meghraj P., Lothe, Rakesh R., Shaligram, Umesh, Johnston, Ryan S., Crowell, Laura E., Castelino, Seraphin, Tracey, Mary Kate, Whittaker, Charles A., Love, J. Christopher · 2021
Full title: Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered <i>Komagataella phaffii</i>
Authors: Dalvie, Neil C., Biedermann, Andrew M., Rodriguez‐Aponte, Sergio A., Naranjo, Christopher A., Rao, Harish, Rajurkar, Meghraj P., Lothe, Rakesh R., Shaligram, Umesh, Johnston, Ryan S., Crowell, Laura E., Castelino, Seraphin, Tracey, Mary Kate, Whittaker, Charles A., Love, J. Christopher
Time-resolved secretome analysis of three <i>Colletotrichum</i> species identifies copper radical alcohol oxidases for the production of fatty aldehydes
Full title: Time-resolved secretome analysis of three <i>Colletotrichum</i> species identifies copper radical alcohol oxidases for the production of fatty aldehydes
P <sub> <i>AOX1</i> </sub> expression in mixed-substrate continuous cultures of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) is completely determined by methanol consumption regardless of the secondary carbon source
Singh, Anamika, Narang, Atul · 2021
Full title: P <sub> <i>AOX1</i> </sub> expression in mixed-substrate continuous cultures of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) is completely determined by methanol consumption regardless of the secondary carbon source
Steric accessibility of the <i>N</i> -terminus improves the titer and quality of recombinant proteins secreted from <i>Komagataella phaffii</i>
Dalvie, Neil C., Naranjo, Christopher A., Rodriguez‐Aponte, Sergio A., Johnston, Ryan S., Love, J. Christopher · 2022
Full title: Steric accessibility of the <i>N</i> -terminus improves the titer and quality of recombinant proteins secreted from <i>Komagataella phaffii</i>
Authors: Dalvie, Neil C., Naranjo, Christopher A., Rodriguez‐Aponte, Sergio A., Johnston, Ryan S., Love, J. Christopher
Identification, characterisation and recombinant expression of flavonoid 3’,5’-hydroxylases and cytochrome P450 reductases from <i>Vaccinium</i> species
Kukk, Kaia · 2023
Full title: Identification, characterisation and recombinant expression of flavonoid 3’,5’-hydroxylases and cytochrome P450 reductases from <i>Vaccinium</i> species
Full title: Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in <i>Komagataella phaffii</i>
The Mut <sup>+</sup> strain of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) expresses P <i> <sub>AOX1</sub> </i> 5 and 10 times faster than Mut <sup>s</sup> and Mut <sup>−</sup> strains: Evidence that formaldehyde or/and formate are true inducers of AOX
Singh, Anamika, Narang, Atul · 2019
Full title: The Mut <sup>+</sup> strain of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) expresses P <i> <sub>AOX1</sub> </i> 5 and 10 times faster than Mut <sup>s</sup> and Mut <sup>−</sup> strains: Evidence that formaldehyde or/and formate are true inducers of AOX
Display of malaria transmission-blocking antigens on chimeric duck hepatitis B virus-derived virus-like particles produced in <i>Hansenula polymorpha</i>
Wetzel, David, Chan, Jo-Anne, Suckow, Manfred, Barbian, Andreas, Weniger, Michael, Jenzelewski, Volker, Reiling, Linda, Richards, Jack S., Anderson, David A., Kouskousis, Betty, Palmer, Catherine S., Hanssen, Eric, Schembecker, Gerhard, Merz, J., Beeson, James G., Piontek, Michael · 2019
Full title: Display of malaria transmission-blocking antigens on chimeric duck hepatitis B virus-derived virus-like particles produced in <i>Hansenula polymorpha</i>
Authors: Wetzel, David, Chan, Jo-Anne, Suckow, Manfred, Barbian, Andreas, Weniger, Michael, Jenzelewski, Volker, Reiling, Linda, Richards, Jack S., Anderson, David A., Kouskousis, Betty, Palmer, Catherine S., Hanssen, Eric, Schembecker, Gerhard, Merz, J., Beeson, James G., Piontek, Michael
Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso
Archangelo, Bruna Samhan · 2017
Full title: Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso
Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1
Ravin, Nikolai V., Eldarov, Michael A., Kadnikov, Vitaly V., Beletsky, Alexey V., Schneider, Jessica, Mardanova, Eugenia S., Smekalova, Elena M., Zvereva, Maria I., Донцова, О. А., Mardanov, Andrey V., Skryabin, K. G. · 2013
Full title: Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1
Authors: Ravin, Nikolai V., Eldarov, Michael A., Kadnikov, Vitaly V., Beletsky, Alexey V., Schneider, Jessica, Mardanova, Eugenia S., Smekalova, Elena M., Zvereva, Maria I., Донцова, О. А., Mardanov, Andrey V., Skryabin, K. G.
The alkaline pectate lyase PEL168 of Bacillus subtilis heterologously expressed in Pichia pastorisis more stable and efficient for degumming ramie fiber
Full title: The alkaline pectate lyase PEL168 of Bacillus subtilis heterologously expressed in Pichia pastorisis more stable and efficient for degumming ramie fiber
Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris
Canales, Mario, Lastra, José Manuel Pérez de la, Naranjo, Victoria, Nijhof, Ard M., Hope, Michelle, Jongejan, Frans, Fuente, José de la · 2008
Full title: Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris
Authors: Canales, Mario, Lastra, José Manuel Pérez de la, Naranjo, Victoria, Nijhof, Ard M., Hope, Michelle, Jongejan, Frans, Fuente, José de la
Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes
Bey, Mathieu, Berrin, Jean‐Guy, Poidevin, Laetitia, Sigoillot, Jean‐Claude · 2011
Full title: Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes
Authors: Bey, Mathieu, Berrin, Jean‐Guy, Poidevin, Laetitia, Sigoillot, Jean‐Claude
Full title: Development of a cultivation process for the enhancement of human interferon alpha 2b production in the oleaginous yeast, Yarrowia lipolytica
Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes
Full title: Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes
Full title: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES
A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter
Nallaseth, Ferez S., Anderson, Stephen K. · 2013
Full title: A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter
Authors: Nallaseth, Ferez S., Anderson, Stephen K.
Towards better understanding of an industrial cell factory: investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris
Fazenda, Mariana L., Dias, João M.L., Harvey, Linda M., Nordon, Alison, Edrada‐Ebel, RuAngelie, Littlejohn, David, McNeil, Brian · 2013
Full title: Towards better understanding of an industrial cell factory: investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris
Authors: Fazenda, Mariana L., Dias, João M.L., Harvey, Linda M., Nordon, Alison, Edrada‐Ebel, RuAngelie, Littlejohn, David, McNeil, Brian
Exometabolome analysis reveals hypoxia at the up-scaling of a Saccharomyces cerevisiae high-cell density fed-batch biopharmaceutical process
Fu, Zhibiao, Verderame, Thomas D, Leighton, Julie, Sampey, Brante P., Appelbaum, Edward R., Patel, Pramatesh S., Aon, Juan C. · 2014
Full title: Exometabolome analysis reveals hypoxia at the up-scaling of a Saccharomyces cerevisiae high-cell density fed-batch biopharmaceutical process
Authors: Fu, Zhibiao, Verderame, Thomas D, Leighton, Julie, Sampey, Brante P., Appelbaum, Edward R., Patel, Pramatesh S., Aon, Juan C.
Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase
Hemmerich, Johannes, Adelantado, Núria, Barrigón, José Manuel, Ponte, Xavier, Hörmann, Astrid, Ferrer, Pau, Kensy, Frank, Valero, Francisco · 2014
Full title: Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase
Authors: Hemmerich, Johannes, Adelantado, Núria, Barrigón, José Manuel, Ponte, Xavier, Hörmann, Astrid, Ferrer, Pau, Kensy, Frank, Valero, Francisco
Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review
Cós, Oriol, Ramón, Ramón, Montesinos, José Luis González, Valero, Francisco · 2006
Full title: Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review
Authors: Cós, Oriol, Ramón, Ramón, Montesinos, José Luis González, Valero, Francisco
Transcriptional analysis of protein production and induction of unfolded protein response in Pichia pastoris expressing a Rhizopus oryzae lipase under the FLD1 promoter
David Resina, M. Bollók, F. Valero, P. Neubauer, P. Ferrer · 2006
Full title: Transcriptional analysis of protein production and induction of unfolded protein response in Pichia pastoris expressing a Rhizopus oryzae lipase under the FLD1 promoter
Authors: David Resina, M. Bollók, F. Valero, P. Neubauer, P. Ferrer
Analysis of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated formaldehyde dehydrogenase promoter (PFLD1)
Full title: Analysis of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated formaldehyde dehydrogenase promoter (PFLD1)
Substrate feeding strategies in Pichia pastoris fed-batch cultivation processes: Analysis of key parameters influencing recombinant protein production
Ramón, Ramón, Cós, Oriol, Ferrer, Pau, Montesinos, José Luis González, Valero, Francisco · 2006
Full title: Substrate feeding strategies in Pichia pastoris fed-batch cultivation processes: Analysis of key parameters influencing recombinant protein production
Authors: Ramón, Ramón, Cós, Oriol, Ferrer, Pau, Montesinos, José Luis González, Valero, Francisco
Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen
Full title: Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen
Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime
Holmes, William J., Darby, Richard A. J., Wilks, Martin DB, Smith, Rodney, Bill, Roslyn M. · 2009
Full title: Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime
Authors: Holmes, William J., Darby, Richard A. J., Wilks, Martin DB, Smith, Rodney, Bill, Roslyn M.
Full title: Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-β-mannosidase from Aspergillus niger BK01
Full title: Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels
Performance of the auxotrophic Saccharomyces cerevisiae BY4741 as host for the production of IL-1β in aerated fed-batch reactor: role of ACA supplementation, strain viability, and maintenance energy
Full title: Performance of the auxotrophic Saccharomyces cerevisiae BY4741 as host for the production of IL-1β in aerated fed-batch reactor: role of ACA supplementation, strain viability, and maintenance energy
Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin
Chandrasekhar Gurramkonda, Sulena Polez, N. Skoko, Ahmad Adnan, Thomas Gäbel, Dipti A. Chugh, S. Swaminathan, N. Khanna, S. Tisminetzky, U. Rinas · 2010
Full title: Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin
Authors: Chandrasekhar Gurramkonda, Sulena Polez, N. Skoko, Ahmad Adnan, Thomas Gäbel, Dipti A. Chugh, S. Swaminathan, N. Khanna, S. Tisminetzky, U. Rinas
Expression of an extremely acidic beta-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence.
Full title: Expression of an extremely acidic beta-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence.
High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture.
Full title: High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture.
Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33
Full title: Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33
A new acidophilic thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris
Full title: A new acidophilic thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris
High-level expression, purification, and enzymatic characterization of truncated human plasminogen (Lys531-Asn791) in the methylotrophic yeast Pichia pastoris
Full title: High-level expression, purification, and enzymatic characterization of truncated human plasminogen (Lys531-Asn791) in the methylotrophic yeast Pichia pastoris
Production of human pro-relaxin H2 in the yeast Pichia pastoris
D. Cimini, K. Corte, R. Finamore, L. Andreozzi, A. Stellavato, A. Pirozzi, F. Ferrara, R. Formisano, M. de Rosa, M. Chino, L. Lista, A. Lombardi, V. Pavone, C. Schiraldi · 2017
Full title: Production of human pro-relaxin H2 in the yeast Pichia pastoris
Authors: D. Cimini, K. Corte, R. Finamore, L. Andreozzi, A. Stellavato, A. Pirozzi, F. Ferrara, R. Formisano, M. de Rosa, M. Chino, L. Lista, A. Lombardi, V. Pavone, C. Schiraldi
Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring
Full title: Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring
Full title: Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation
Heterologous expression and biochemical characterization of a GHF9 endoglucanase from the termite Reticulitermes speratus in Pichia pastoris
Zhang, Pengfei, Yuan, Xianghua, Du, Yuguang, Li, Jianjun · 2018
Full title: Heterologous expression and biochemical characterization of a GHF9 endoglucanase from the termite Reticulitermes speratus in Pichia pastoris
Authors: Zhang, Pengfei, Yuan, Xianghua, Du, Yuguang, Li, Jianjun
An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)
Che, Zhiqun, Cao, Xiaoyan, Chen, Guiguang, Liang, Zhiqun · 2020
Full title: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)
Authors: Che, Zhiqun, Cao, Xiaoyan, Chen, Guiguang, Liang, Zhiqun
Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks
Bawa, Zharain, Routledge, Sarah J, Jamshad, Mohammed, Clare, Michelle, Sarkar, Debasmita, Dickerson, Ian M., Ganzlin, Markus, Poyner, David R., Bill, Roslyn M. · 2014
Full title: Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks
Authors: Bawa, Zharain, Routledge, Sarah J, Jamshad, Mohammed, Clare, Michelle, Sarkar, Debasmita, Dickerson, Ian M., Ganzlin, Markus, Poyner, David R., Bill, Roslyn M.
High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities
Full title: High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities
Full title: High-temperature cultivation of recombinant Pichia pastoris increases endoplasmic reticulum stress and decreases production of human interleukin-10.
Integrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger
Full title: Integrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger
Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source
Schotte, Peter, Dewerte, Isabelle, Groeve, Manu De, Keyser, Saskia De, Brabandere, Veronique De, Stanssens, Patrick · 2016
Full title: Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source
Authors: Schotte, Peter, Dewerte, Isabelle, Groeve, Manu De, Keyser, Saskia De, Brabandere, Veronique De, Stanssens, Patrick
Full title: Optimization of construct design and fermentation strategy for the production of bioactive ATF-SAP, a saporin based anti-tumoral uPAR-targeted chimera
Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems
Ma, Su, Preims, Marita, Piumi, François, Kappel, Lisa, Seiboth, Bernhard, Record, Éric, Kracher, Daniel, Ludwig, Roland · 2017
Full title: Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems
Authors: Ma, Su, Preims, Marita, Piumi, François, Kappel, Lisa, Seiboth, Bernhard, Record, Éric, Kracher, Daniel, Ludwig, Roland
Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris
Moser, Josef W., Prielhofer, Roland, Gerner, Samuel M., Graf, Alexandra B., Wilson, Iain B. H., Mattanovich, Diethard, Dragosits, Martin · 2017
Full title: Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris
Authors: Moser, Josef W., Prielhofer, Roland, Gerner, Samuel M., Graf, Alexandra B., Wilson, Iain B. H., Mattanovich, Diethard, Dragosits, Martin
Full title: Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison.
Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection.
Bando-Campos, Giroshi, Juárez-López, Daniel, Román-González, Sergio A, Castillo-Rodal, Antonia I, Olvera, Clarita, López-Vidal, Yolanda, Arreguín-Espinosa, Roberto, Espitia, Clara, Trujillo-Roldán, Mauricio A, Valdez-Cruz, Norma A · 2019
Full title: Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection.
Authors: Bando-Campos, Giroshi, Juárez-López, Daniel, Román-González, Sergio A, Castillo-Rodal, Antonia I, Olvera, Clarita, López-Vidal, Yolanda, Arreguín-Espinosa, Roberto, Espitia, Clara, Trujillo-Roldán, Mauricio A, Valdez-Cruz, Norma A
Full title: Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris
Characterization of a highly stable α-galactosidase from thermophilic Rasamsonia emersonii heterologously expressed in a modified Pichia pastoris expression system
Full title: Characterization of a highly stable α-galactosidase from thermophilic Rasamsonia emersonii heterologously expressed in a modified Pichia pastoris expression system
Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage
Garrigós‐Martínez, Javier, Nieto‐Taype, Miguel Angel, Gasset-Franch, Arnau, Montesinos, José Luis González, Garcia‐Ortega, Xavier, Valero, Francisco · 2019
Full title: Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage
Authors: Garrigós‐Martínez, Javier, Nieto‐Taype, Miguel Angel, Gasset-Franch, Arnau, Montesinos, José Luis González, Garcia‐Ortega, Xavier, Valero, Francisco
Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone
Full title: Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone
Full title: Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc
Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene
Aggarwal, Sakshi, Mishra, Saroj · 2020
Full title: Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene
Production of bio-xylitol from d-xylose by an engineered Pichia pastoris expressing a recombinant xylose reductase did not require any auxiliary substrate as electron donor
Louie, Tai Man, Louie, Kailin, DenHartog, Samuel, Gopishetty, Sridhar, Subramanian, Mani, Arnold, Mark A., Das, Shuvendu · 2021
Full title: Production of bio-xylitol from d-xylose by an engineered Pichia pastoris expressing a recombinant xylose reductase did not require any auxiliary substrate as electron donor
Authors: Louie, Tai Man, Louie, Kailin, DenHartog, Samuel, Gopishetty, Sridhar, Subramanian, Mani, Arnold, Mark A., Das, Shuvendu
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher · 2021
Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Authors: Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher
Full title: Simultaneous enzyme production, Levan-type FOS synthesis and sugar by-products elimination using a recombinant Pichia pastoris strain expressing a levansucrase-endolevanase fusion enzyme
High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux
Full title: High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux
‘Mother(Nature) knows best’ – hijacking nature-designed transcriptional programs for enhancing stress resistance and protein production in Yarrowia lipolytica; presentation of YaliFunTome database
Gorczyca, Maria, Białas, W., Nicaud, Jean‐Marc, Celińska, Ewelina · 2024
Full title: ‘Mother(Nature) knows best’ – hijacking nature-designed transcriptional programs for enhancing stress resistance and protein production in Yarrowia lipolytica; presentation of YaliFunTome database
Authors: Gorczyca, Maria, Białas, W., Nicaud, Jean‐Marc, Celińska, Ewelina
Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications
Full title: Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications
Full title: Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii
CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Dalvie, Neil C., Lorgeree, Timothy, Yang, Yuchen, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Hinckley, Joshua A., Clark, John J, Rosario, Amanda Del, Love, Kerry R., Love, J. Christopher · 2024
Full title: CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Authors: Dalvie, Neil C., Lorgeree, Timothy, Yang, Yuchen, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Hinckley, Joshua A., Clark, John J, Rosario, Amanda Del, Love, Kerry R., Love, J. Christopher
Development of an efficient expression system for human chaperone BiP in Pichia pastoris: production optimization and functional validation
Eimantas Žitkus, E. Ciplys, Mantas Žiaunys, A. Sakalauskas, R. Slibinskas · 2025
Full title: Development of an efficient expression system for human chaperone BiP in Pichia pastoris: production optimization and functional validation
Authors: Eimantas Žitkus, E. Ciplys, Mantas Žiaunys, A. Sakalauskas, R. Slibinskas
Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris
Navone, Laura, Vogl, Thomas, Luangthongkam, Pawarisa, Blinco, Jo-Anne, Luna‐Flores, Carlos H., Chen, Xiaojing, Hellens, Juhani von, Mahler, Stephen M., Speight, Robert · 2021
Full title: Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris
Authors: Navone, Laura, Vogl, Thomas, Luangthongkam, Pawarisa, Blinco, Jo-Anne, Luna‐Flores, Carlos H., Chen, Xiaojing, Hellens, Juhani von, Mahler, Stephen M., Speight, Robert
Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution
Kao, Mu‐Rong, Yu, Su‐May, Ho, Tuan-H ua David · 2021
Full title: Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution
Authors: Kao, Mu‐Rong, Yu, Su‐May, Ho, Tuan-H ua David
Full title: Pichia pastoris versus Saccharomyces cerevisiae: a case study on the recombinant production of human granulocyte-macrophage colony-stimulating factor.
Full title: Expression of a highly active β-glucosidase from Aspergillus niger AS3.4523 in Escherichia coli and its application in gardenia blue preparation
Coexpression of cellulases in Pichia pastoris as a self-processing protein fusion
Araújo, Juliana de Amorim, Ferreira, Túlio César, Rubini, Marciano Régis, Duran, Ana Gilhema Gomez, Marco, Janice Lisboa De, Moraes, Lídia Maria Pepe de, Torres, Fernando Araripe Gonçalves · 2015
Full title: Coexpression of cellulases in Pichia pastoris as a self-processing protein fusion
Authors: Araújo, Juliana de Amorim, Ferreira, Túlio César, Rubini, Marciano Régis, Duran, Ana Gilhema Gomez, Marco, Janice Lisboa De, Moraes, Lídia Maria Pepe de, Torres, Fernando Araripe Gonçalves
Expression of a new laccase from Moniliophthora roreri at high levels in Pichia pastoris and its potential application in micropollutant degradation
Bronikowski, Agathe, Hagedoorn, Peter‐Leon, Koschorreck, Katja, Urlacher, Vlada B. · 2017
Full title: Expression of a new laccase from Moniliophthora roreri at high levels in Pichia pastoris and its potential application in micropollutant degradation
Authors: Bronikowski, Agathe, Hagedoorn, Peter‐Leon, Koschorreck, Katja, Urlacher, Vlada B.
Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability.
Kangwa, Martin, Salgado, Jose Antonio Gama, Fernandez-Lahore, Hector Marcelo · 2018
Full title: Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability.
Authors: Kangwa, Martin, Salgado, Jose Antonio Gama, Fernandez-Lahore, Hector Marcelo
Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris
Full title: Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris
Enhancing the secretion pathway maximizes the effects of mixed feeding strategy for glucose oxidase production in the methylotrophic yeast Pichia pastoris
Full title: Enhancing the secretion pathway maximizes the effects of mixed feeding strategy for glucose oxidase production in the methylotrophic yeast Pichia pastoris
Improved microscale cultivation of Pichia pastoris for clonal screening
Eck, Alexander, Schmidt, Matthias, Hamer, Stefanie Nicole, Ruff, Anna Joëlle, Förster, Jan Janosch, Schwaneberg, Ulrich, Blank, Lars M., Wiechert, Wolfgang, Oldiges, Marco · 2018
Full title: Improved microscale cultivation of Pichia pastoris for clonal screening
Authors: Eck, Alexander, Schmidt, Matthias, Hamer, Stefanie Nicole, Ruff, Anna Joëlle, Förster, Jan Janosch, Schwaneberg, Ulrich, Blank, Lars M., Wiechert, Wolfgang, Oldiges, Marco
An improved expression and purification protocol enables the structural characterization of Mnt1, an antifungal target from Candida albicans.
Silva, Patrícia Alves, Souza, Amanda Araújo, de Oliveira, Gideane Mendes, Ramada, Marcelo Henrique Soller, Hernández, Nahúm Valente, Mora-Montes, Héctor Manuel, Bueno, Renata Vieira, Martins-de-Sa, Diogo, de Freitas, Sonia Maria, Felipe, Maria Sueli Soares, Barbosa, João Alexandre Ribeiro Gonçalves · 2024
Full title: An improved expression and purification protocol enables the structural characterization of Mnt1, an antifungal target from Candida albicans.
Authors: Silva, Patrícia Alves, Souza, Amanda Araújo, de Oliveira, Gideane Mendes, Ramada, Marcelo Henrique Soller, Hernández, Nahúm Valente, Mora-Montes, Héctor Manuel, Bueno, Renata Vieira, Martins-de-Sa, Diogo, de Freitas, Sonia Maria, Felipe, Maria Sueli Soares, Barbosa, João Alexandre Ribeiro Gonçalves
Rational Design of a Fibroblast Growth Factor 21-Based Clinical Candidate, LY2405319
Kharitonenkov, Alexei, Beals, John M., Micanovic, Radmila, Strifler, Beth A., Rathnachalam, Radhakrishnan, Wroblewski, Victor J., Li, Shun, Köester, Anja, Ford, Amy, Coşkun, Tamer, Dunbar, James D., Cheng, Christine C., Frye, Christopher C., Bumol, Thomas F., Moller, David E. · 2013
Full title: Rational Design of a Fibroblast Growth Factor 21-Based Clinical Candidate, LY2405319
Authors: Kharitonenkov, Alexei, Beals, John M., Micanovic, Radmila, Strifler, Beth A., Rathnachalam, Radhakrishnan, Wroblewski, Victor J., Li, Shun, Köester, Anja, Ford, Amy, Coşkun, Tamer, Dunbar, James D., Cheng, Christine C., Frye, Christopher C., Bumol, Thomas F., Moller, David E.
Full title: Detergent Screening and Purification of the Human Liver ABC Transporters BSEP (ABCB11) and MDR3 (ABCB4) Expressed in the Yeast Pichia pastoris
Characterization of the Pichia pastoris Protein-O-mannosyltransferase Gene Family
Nett, Juergen H., Cook, W. James, Mingtang, Chen, Davidson, Robert C., Bobrowicz, Piotr, Kett, Warren C., Brevnova, Elena E., Potgieter, Thomas I., Mellon, Mark T., Prinz, Bianka, Choi, Byung‐Kwon, Zha, Dongxing, Burnina, Irina, Bukowski, John, Du, Min, Wildt, Stefan, Hamilton, Stephen R. · 2013
Full title: Characterization of the Pichia pastoris Protein-O-mannosyltransferase Gene Family
Authors: Nett, Juergen H., Cook, W. James, Mingtang, Chen, Davidson, Robert C., Bobrowicz, Piotr, Kett, Warren C., Brevnova, Elena E., Potgieter, Thomas I., Mellon, Mark T., Prinz, Bianka, Choi, Byung‐Kwon, Zha, Dongxing, Burnina, Irina, Bukowski, John, Du, Min, Wildt, Stefan, Hamilton, Stephen R.
Glycosylation variants of a β-glucosidase secreted by a Taiwanese fungus, Chaetomella raphigera, exhibit variant-specific catalytic and biochemical properties.
Full title: Glycosylation variants of a β-glucosidase secreted by a Taiwanese fungus, Chaetomella raphigera, exhibit variant-specific catalytic and biochemical properties.
Purification, cloning, characterization, and N-glycosylation analysis of a novel β-fructosidase from Aspergillus oryzae FS4 synthesizing levan- and neolevan-type fructooligosaccharides.
Full title: Purification, cloning, characterization, and N-glycosylation analysis of a novel β-fructosidase from Aspergillus oryzae FS4 synthesizing levan- and neolevan-type fructooligosaccharides.
Computational Analysis and Low-Scale Constitutive Expression of Laccases Synthetic Genes GlLCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris
Rivera-Hoyos, Claudia M., Morales-Álvarez, Edwin D., Cuevas, Sergio Alejandro Poveda, Reyes-Guzmán, Edwin Alfredo, Poutou‐Piñales, Raúl A., Reyes-Montaño, Edgar Antonio, Pedroza-Rodríguez, Aura M., Rodríguez-Vázquez, Refugio, Cardozo-Bernal, Ángela M. · 2015
Full title: Computational Analysis and Low-Scale Constitutive Expression of Laccases Synthetic Genes GlLCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris
Authors: Rivera-Hoyos, Claudia M., Morales-Álvarez, Edwin D., Cuevas, Sergio Alejandro Poveda, Reyes-Guzmán, Edwin Alfredo, Poutou‐Piñales, Raúl A., Reyes-Montaño, Edgar Antonio, Pedroza-Rodríguez, Aura M., Rodríguez-Vázquez, Refugio, Cardozo-Bernal, Ángela M.
Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
Edwards-Jones, Bryn, Aw, Rochelle, Barton, Geraint, Tredwell, Gregory D., Bundy, Jacob G., Leak, David J. · 2015
Full title: Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
Authors: Edwards-Jones, Bryn, Aw, Rochelle, Barton, Geraint, Tredwell, Gregory D., Bundy, Jacob G., Leak, David J.
Overexpression of a Novel Thermostable and Chloride-Tolerant Laccase from Thermus thermophilus SG0.5JP17-16 in Pichia pastoris and Its Application in Synthetic Dye Decolorization
Full title: Overexpression of a Novel Thermostable and Chloride-Tolerant Laccase from Thermus thermophilus SG0.5JP17-16 in Pichia pastoris and Its Application in Synthetic Dye Decolorization
High-Level Expression of Endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Application for the Deglycosylation of Glycoproteins
Full title: High-Level Expression of Endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Application for the Deglycosylation of Glycoproteins
Characterization of a Recombinant Cathepsin B-Like Cysteine Peptidase from Diaphorina citri Kuwayama (Hemiptera: Liviidae): A Putative Target for Control of Citrus Huanglongbing
Ferrara, Taíse Fernanda da Silva, Schneider, Vanessa Karine, Kishi, Luciano Takeshi, Carmona, Adriana K., Alves, Márcio F.M., Belasque, José, Rosa, José Cesar, Hunter, Wayne B., Henrique‐Silva, Flávio, Soares‐Costa, Andrea · 2015
Full title: Characterization of a Recombinant Cathepsin B-Like Cysteine Peptidase from Diaphorina citri Kuwayama (Hemiptera: Liviidae): A Putative Target for Control of Citrus Huanglongbing
Authors: Ferrara, Taíse Fernanda da Silva, Schneider, Vanessa Karine, Kishi, Luciano Takeshi, Carmona, Adriana K., Alves, Márcio F.M., Belasque, José, Rosa, José Cesar, Hunter, Wayne B., Henrique‐Silva, Flávio, Soares‐Costa, Andrea
Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E. coli.
Full title: Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E. coli.
Integration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism
Tomàs‐Gamisans, Màrius, Ferrer, Pau, Albiol, Joan · 2016
Full title: Integration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism
Authors: Tomàs‐Gamisans, Màrius, Ferrer, Pau, Albiol, Joan
Full title: Role of N-linked glycosylation in the enzymatic properties of a thermophilic GH 10 xylanase from Aspergillus fumigatus expressed in Pichia pastoris.
Full title: A quantitative indicator diagram for lytic polysaccharide monooxygenases reveals the role of aromatic surface residues in HjLPMO9A regioselectivity
Enhancing monellin production by Pichia pastoris at low cell induction concentration via effectively regulating methanol metabolism patterns and energy utilization efficiency
Full title: Enhancing monellin production by Pichia pastoris at low cell induction concentration via effectively regulating methanol metabolism patterns and energy utilization efficiency
Full title: Modulation of heterologous protein secretion in the thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC 656 by CRISPR-Cas9 system
Expression of Pisum sativum defensin 1 (Psd1) in shaking flasks and bioreactor cultivations of recombinant Pichia pastoris at different pHs
Larentis, Ariane Leites, Almeida, Marcius S., Cabral, Kátia M.S., Medeiros, Luciano Neves de, Kurtenbach, Eleonora, Coelho, Maria Alice Zarur · 2004
Full title: Expression of Pisum sativum defensin 1 (Psd1) in shaking flasks and bioreactor cultivations of recombinant Pichia pastoris at different pHs
Authors: Larentis, Ariane Leites, Almeida, Marcius S., Cabral, Kátia M.S., Medeiros, Luciano Neves de, Kurtenbach, Eleonora, Coelho, Maria Alice Zarur
Constitutive High Expression Level of A Synthetic Deleted Encoding Gene of <i>Talaromyces minioluteus</i> Endodextranase Variant (r-TmDEX49A-ΔSP-ΔN30) in <i>Komagataella phaffii</i> (<i>Pichia pastoris</i>)
Full title: Constitutive High Expression Level of A Synthetic Deleted Encoding Gene of <i>Talaromyces minioluteus</i> Endodextranase Variant (r-TmDEX49A-ΔSP-ΔN30) in <i>Komagataella phaffii</i> (<i>Pichia pastoris</i>)
Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain (RBD) Using Pichia pastoris in Stirred-Tank Bioreactor
Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, María Florencia, Wetzler, Diana E., Gentilli, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas AM · 2023
Full title: Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain (RBD) Using Pichia pastoris in Stirred-Tank Bioreactor
Authors: Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, María Florencia, Wetzler, Diana E., Gentilli, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas AM
Full title: Production of Plant-Based Meat Flavor Compound – Leghemo-Globin (LegH) in Recombinant <em>Komagataella phaffii</em> Fed-Batch Fermentations
State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
Full title: State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
Establishing site-specific recombination mediated cassette exchange in Pichia pastoris and development of an optimized process for the production of mouse Tmprss2
Schinkowski, Christian · 2017
Full title: Establishing site-specific recombination mediated cassette exchange in Pichia pastoris and development of an optimized process for the production of mouse Tmprss2
Identification and Characterization of a Novel Hyperthermostable Bifunctional Cellobiohydrolase- Xylanase Enzyme for Synergistic Effect With Commercial Cellulase on Pretreated Wheat Straw Degradation
Full title: Identification and Characterization of a Novel Hyperthermostable Bifunctional Cellobiohydrolase- Xylanase Enzyme for Synergistic Effect With Commercial Cellulase on Pretreated Wheat Straw Degradation
High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming
Full title: High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming
Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
Gasset, Arnau, Garcia‐Ortega, Xavier, Garrigós‐Martínez, Javier, Valero, Francisco, Montesinos, José Luis González · 2022
Full title: Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
Authors: Gasset, Arnau, Garcia‐Ortega, Xavier, Garrigós‐Martínez, Javier, Valero, Francisco, Montesinos, José Luis González
PAOX1 expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determined by methanol consumption regardless of the secondary carbon source
Singh, Anamika, Narang, Atul · 2023
Full title: PAOX1 expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determined by methanol consumption regardless of the secondary carbon source
Vaccine Containing the Three Allelic Variants of the Plasmodium vivax Circumsporozoite Antigen Induces Protection in Mice after Challenge with a Transgenic Rodent Malaria Parasite
Full title: Vaccine Containing the Three Allelic Variants of the Plasmodium vivax Circumsporozoite Antigen Induces Protection in Mice after Challenge with a Transgenic Rodent Malaria Parasite
Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis.
Vici, Ana C, da Cruz, Andrezza F, Facchini, Fernanda D A, de Carvalho, Caio C, Pereira, Marita G, Fonseca-Maldonado, Raquel, Ward, Richard J, Pessela, Benevides C, Fernandez-Lorente, Gloria, Torres, Fernando A G, Jorge, João A, Polizeli, Maria L T M · 2015
Full title: Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis.
Authors: Vici, Ana C, da Cruz, Andrezza F, Facchini, Fernanda D A, de Carvalho, Caio C, Pereira, Marita G, Fonseca-Maldonado, Raquel, Ward, Richard J, Pessela, Benevides C, Fernandez-Lorente, Gloria, Torres, Fernando A G, Jorge, João A, Polizeli, Maria L T M
A Review of the Microbial Production of Bioactive Natural Products and Biologics
Pham, Janette V., Yilma, Mariamawit A., Feliz, Adriana, Majid, Murtadha T., Maffetone, Nicholas, Walker, Jorge R., Kim, Eunji, Cho, Hyo Je, Reynolds, Jared M., Song, Myoung‐Chong, Park, Sung Ryeol, Yoon, Yeo Joon · 2019
Full title: A Review of the Microbial Production of Bioactive Natural Products and Biologics
Authors: Pham, Janette V., Yilma, Mariamawit A., Feliz, Adriana, Majid, Murtadha T., Maffetone, Nicholas, Walker, Jorge R., Kim, Eunji, Cho, Hyo Je, Reynolds, Jared M., Song, Myoung‐Chong, Park, Sung Ryeol, Yoon, Yeo Joon
Full title: Gene Expression, Biochemical Characterization of a sn-1, 3 Extracellular Lipase From Aspergillus niger GZUF36 and Its Model-Structure Analysis
Data_Sheet_1_Heterologous Expression and Characterization of a High-Efficiency Chitosanase From Bacillus mojavensis SY1 Suitable for Production of Chitosan Oligosaccharides.docx
Full title: Data_Sheet_1_Heterologous Expression and Characterization of a High-Efficiency Chitosanase From Bacillus mojavensis SY1 Suitable for Production of Chitosan Oligosaccharides.docx
Overexpression and Biochemical Properties of a GH46 Chitosanase From Marine Streptomyces hygroscopicus R1 Suitable for Chitosan Oligosaccharides Preparation
Full title: Overexpression and Biochemical Properties of a GH46 Chitosanase From Marine Streptomyces hygroscopicus R1 Suitable for Chitosan Oligosaccharides Preparation
Biochemical analyses of a novel acidophilic GH5 β-mannanase from Trichoderma asperellum ND-1 and its application in mannooligosaccharides production from galactomannans
Full title: Biochemical analyses of a novel acidophilic GH5 β-mannanase from Trichoderma asperellum ND-1 and its application in mannooligosaccharides production from galactomannans
From linoleic acid to hexanal and hexanol by whole cell catalysis with a lipoxygenase, hydroperoxide lyase and reductase cascade in Komagataella phaffii
Hashem, Chiam, Hochrinner, Julius, Bürgler, Moritz B., Rinnofner, Claudia, Pichler, Harald, Winkler, Margit · 2022
Full title: From linoleic acid to hexanal and hexanol by whole cell catalysis with a lipoxygenase, hydroperoxide lyase and reductase cascade in Komagataella phaffii
Authors: Hashem, Chiam, Hochrinner, Julius, Bürgler, Moritz B., Rinnofner, Claudia, Pichler, Harald, Winkler, Margit
Full title: Preparative Production of Functionalized (N- and O-Heterocyclic) Polycyclic Aromatic Hydrocarbons by Human Cytochrome P450 3A4 in a Bioreactor
Specific Phenylpropanoid Oligomerization in a Neutral Environment by the Recombinant Alkaline Laccase from Paramyrothecium roridum VKM F-3565.
Renfeld, Zhanna V, Chernykh, Alexey M, Gorina, Sofia Yu, Baskunov, Boris P, Moiseeva, Olga V, Trachtmann, Natalia V, Validov, Shamil Z, Kolomytseva, Marina P · 2025
Full title: Specific Phenylpropanoid Oligomerization in a Neutral Environment by the Recombinant Alkaline Laccase from Paramyrothecium roridum VKM F-3565.
Authors: Renfeld, Zhanna V, Chernykh, Alexey M, Gorina, Sofia Yu, Baskunov, Boris P, Moiseeva, Olga V, Trachtmann, Natalia V, Validov, Shamil Z, Kolomytseva, Marina P
Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris
Maity, Nitu, Mishra, Saroj · 2019
Full title: Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris
Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi
Birchfield, Aaron S., McIntosh, Cecilia A. · 2024
Full title: Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi
Authors: Birchfield, Aaron S., McIntosh, Cecilia A.
Optimization of Bioethanol In Silico Production Process in a Fed-Batch Bioreactor Using Non-Linear Model Predictive Control and Evolutionary Computation Techniques
Freitas, Hanniel Ferreira Sarmento de, Olivo, José Eduardo, Andrade, Cid Marcos Gonçalves · 2017
Full title: Optimization of Bioethanol In Silico Production Process in a Fed-Batch Bioreactor Using Non-Linear Model Predictive Control and Evolutionary Computation Techniques
Authors: Freitas, Hanniel Ferreira Sarmento de, Olivo, José Eduardo, Andrade, Cid Marcos Gonçalves
Full title: Performance of Recombinant Komagataella phaffii in Plant-Based Meat Flavor Compound-Leghemoglobin (LegH) Production through Fed-Batch Fermentations
State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
Full title: State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
Enhancing Antimicrobial Peptide Productivity in Pichia pastoris (Muts Strain) by Improving the Fermentation Process Based on Increasing the Volumetric Methanol Consumption Rate
Full title: Enhancing Antimicrobial Peptide Productivity in Pichia pastoris (Muts Strain) by Improving the Fermentation Process Based on Increasing the Volumetric Methanol Consumption Rate
Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain Using Pichia pastoris in Stirred-Tank Bioreactor
Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, Florencia, Wetzler, Diana E., Gentili, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas · 2023
Full title: Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain Using Pichia pastoris in Stirred-Tank Bioreactor
Authors: Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, Florencia, Wetzler, Diana E., Gentili, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas
Lipase from Yarrowia lipolytica: Prospects as an Industrial Biocatalyst for Biotechnological Applications
Silva, Jessica Lopes da, Sales, Misael Bessa, Bizerra, Viviane de Castro, Nobre, Millena Mara Rabelo, Braz, Ana Kátia de Sousa, Sousa, Patrick da Silva, Cavalcante, Antônio Luthierre Gama, Melo, Rafael Leandro Fernandes, Sousa, Paulo Gonçalves de, Neto, Francisco Simão, Fonseca, Aluísio Marques da, Santos, José Cleiton Sousa dos · 2023
Full title: Lipase from Yarrowia lipolytica: Prospects as an Industrial Biocatalyst for Biotechnological Applications
Authors: Silva, Jessica Lopes da, Sales, Misael Bessa, Bizerra, Viviane de Castro, Nobre, Millena Mara Rabelo, Braz, Ana Kátia de Sousa, Sousa, Patrick da Silva, Cavalcante, Antônio Luthierre Gama, Melo, Rafael Leandro Fernandes, Sousa, Paulo Gonçalves de, Neto, Francisco Simão, Fonseca, Aluísio Marques da, Santos, José Cleiton Sousa dos
Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3
Full title: Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3
Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endothelial Growth Factor165 and Programmed Death-1 in Pichia pastoris and Evaluation of Antitumor Activity In Vivo
Full title: Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endothelial Growth Factor165 and Programmed Death-1 in Pichia pastoris and Evaluation of Antitumor Activity In Vivo
Heterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
Zadorozhny, A. V., Ushakov, Viktor Sergeevich, Розанов, А. С., Bogacheva, Natalia Vladimirovna, Shlyakhtun, Valeria Nikolayevna, Voskoboev, Mikhail Evgenyevich, Korzhuk, Anton V., Romancev, Vladislav Anatolevich, Bannikova, Svetlana V., Mescheryakova, Irina Anatolyevna, Антонов, Е. В., Vasilieva, Asya R., Pavlova, Elena Iurevna, Chesnokov, Danil Olegovich, Shedko, Elizaveta Dmitrievna, Bryanskaya, A. V., Bochkov, D. V., Goryachkovskaya, T. N., Peltek, S. E. · 2022
Full title: Heterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
Authors: Zadorozhny, A. V., Ushakov, Viktor Sergeevich, Розанов, А. С., Bogacheva, Natalia Vladimirovna, Shlyakhtun, Valeria Nikolayevna, Voskoboev, Mikhail Evgenyevich, Korzhuk, Anton V., Romancev, Vladislav Anatolevich, Bannikova, Svetlana V., Mescheryakova, Irina Anatolyevna, Антонов, Е. В., Vasilieva, Asya R., Pavlova, Elena Iurevna, Chesnokov, Danil Olegovich, Shedko, Elizaveta Dmitrievna, Bryanskaya, A. V., Bochkov, D. V., Goryachkovskaya, T. N., Peltek, S. E.
A New Expression System Based on Psychrotolerant Debaryomyces macquariensis Yeast and Its Application to the Production of Cold-Active β-d-Galactosidase from Paracoccus sp. 32d
Full title: A New Expression System Based on Psychrotolerant Debaryomyces macquariensis Yeast and Its Application to the Production of Cold-Active β-d-Galactosidase from Paracoccus sp. 32d
Full title: Enhancing the Heterologous Expression of a Thermophilic Endoglucanase and Its Cost-Effective Production in Pichia pastoris Using Multiple Strategies
Production of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unveiling Antimicrobial and Anticancer Functionalities.
Full title: Production of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unveiling Antimicrobial and Anticancer Functionalities.
An Interplay between Transcription Factors and Recombinant Protein Synthesis in Yarrowia lipolytica at Transcriptional and Functional Levels—The Global View
Full title: An Interplay between Transcription Factors and Recombinant Protein Synthesis in Yarrowia lipolytica at Transcriptional and Functional Levels—The Global View
Heterologous Production, Purification and Characterization of Two Cold-Active β-d-Galactosidases with Transglycosylation Activity from the Psychrotolerant Arctic Bacterium Arthrobacter sp. S3* Isolated from Spitsbergen Island Soil.
Wanarska, Marta, Pawlak-Szukalska, Anna, Rosińska, Aleksandra, Kozłowska-Tylingo, Katarzyna · 2024
Full title: Heterologous Production, Purification and Characterization of Two Cold-Active β-d-Galactosidases with Transglycosylation Activity from the Psychrotolerant Arctic Bacterium Arthrobacter sp. S3* Isolated from Spitsbergen Island Soil.
Authors: Wanarska, Marta, Pawlak-Szukalska, Anna, Rosińska, Aleksandra, Kozłowska-Tylingo, Katarzyna
Sustainability Assessment in Recombinant Human Insulin Production—Evaluating the Environmental Impacts of Microbial Growth Medium Components and Formulations
Chatzipanagiotou, Konstantina‐Roxani, Pappas, Alex G., Petrakli, Foteini, Antonaropoulos, George, Koumoulos, Elias P. · 2025
Full title: Sustainability Assessment in Recombinant Human Insulin Production—Evaluating the Environmental Impacts of Microbial Growth Medium Components and Formulations
Authors: Chatzipanagiotou, Konstantina‐Roxani, Pappas, Alex G., Petrakli, Foteini, Antonaropoulos, George, Koumoulos, Elias P.
Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris
Jordà, Joel, Rojas, Hugo, Carnicer, Marc, Wahl, A., Ferrer, Pau, Albiol, Joan · 2014
Full title: Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris
Authors: Jordà, Joel, Rojas, Hugo, Carnicer, Marc, Wahl, A., Ferrer, Pau, Albiol, Joan
Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Хлебодарова, Т. М., Bogacheva, N. V., Zadorozhny, A. V., Bryanskaya, A. V., Vasilieva, Asya R., Chesnokov, Danil Olegovich, Pavlova, Elena I., Peltek, S. E. · 2024
Full title: Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Authors: Хлебодарова, Т. М., Bogacheva, N. V., Zadorozhny, A. V., Bryanskaya, A. V., Vasilieva, Asya R., Chesnokov, Danil Olegovich, Pavlova, Elena I., Peltek, S. E.
Evaluation of product distribution in chemostat and batch fermentation in lactic acid-producing komagataella phaffii strains utilizing glycerol as substrate
Parachin, Nádia Skorupa · 2020
Full title: Evaluation of product distribution in chemostat and batch fermentation in lactic acid-producing komagataella phaffii strains utilizing glycerol as substrate
Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso
Russo, Elisa Maria de Sousa · 2017
Full title: Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso
High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
Blanchard, Véronique; Gadkari, R.P; George, A.V.E. et al. · 2008
Full title: High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain
Brady, Joseph R; Whittaker, Charles A; Tan, Melody C et al. · 2021
Full title: Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain
Authors: Brady, Joseph R; Whittaker, Charles A; Tan, Melody C; Kristensen, D Lee; Ma, Duanduan; Dalvie, Neil C; Love, Kerry Routenberg; Love, J Christopher
CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Dalvie, Neil C.; Lorgeree, Timothy R.; Yang, Yuchen et al. · 2024
Full title: CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome
Authors: Dalvie, Neil C.; Lorgeree, Timothy R.; Yang, Yuchen; Rodriguez-Aponte, Sergio A.; Whittaker, Charles A.; Hinckley, Joshua A.; Clark, John J.; Del Rosario, Amanda M.; Love, Kerry R.; Love, J. C.
Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
Manderson, Daniel · 2017
Full title: Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
Process optimization and scale-up for the production of a diagnostic monoclonal antibody against congenital adrenal hyperplasia (CAH) : a thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Bioprocess Engineering at Massey University, Manawatu Campus, New Zealand
Chua, Gek Kee · 2011
Full title: Process optimization and scale-up for the production of a diagnostic monoclonal antibody against congenital adrenal hyperplasia (CAH) : a thesis presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Bioprocess Engineering at Massey University, Manawatu Campus, New Zealand
Kinetic study of fed-batch and continuous bioconversion processes for L-Phenylacetylcarbinol (L-PAC) production by the yeast Candida utilis
Wang, Bin · 1993
Full title: Kinetic study of fed-batch and continuous bioconversion processes for L-Phenylacetylcarbinol (L-PAC) production by the yeast Candida utilis
Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter
Barrigón de San Marcos, José Manuel · 2015
Full title: Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter
Utilización de glicerol proveniente de la industria de biodiesel como fuente de carbono para la producción de lipasas recombinantes en Pichia pastoris
Loango Chamorro, Nelsy · 2015
Full title: Utilización de glicerol proveniente de la industria de biodiesel como fuente de carbono para la producción de lipasas recombinantes en Pichia pastoris
A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches
Garcia Ortega, Xavier · 2016
Full title: A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches
L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic
Cámara Rey, Elena · 2017
Full title: L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic
Estratègies d’operació per a la producció de lipases de Rhizopus oryzae amb el fenotip Mut(s) de Pichia pastoris mitjançant substrats mixtes
Arnau Jiménez, Carol · 2011
Full title: Estratègies d’operació per a la producció de lipases de Rhizopus oryzae amb el fenotip Mut(s) de Pichia pastoris mitjançant substrats mixtes
Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris
Nieto Taype, Miguel Angel · 2020
Full title: Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris
Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris
Garrigós Martínez, Javier · 2021
Full title: Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris
Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess
Gasset Franch, Arnau · 2024
Full title: Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess