Strain Engineering For Pichia Pastoris Protein Sec

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Paper Secreted production of an elastin-like polypeptide by Pichia pastoris

Schipperus, Roelof; Teeuwen, Rosalie L. M.; Werten, Marc W. T. et al. · 2009 · Applied Microbiology and Biotechnology · DOI

Full title: Secreted production of an elastin-like polypeptide by Pichia pastoris

Authors: Schipperus, Roelof; Teeuwen, Rosalie L. M.; Werten, Marc W. T.; Eggink, Gerrit; Wolf, Frits A. de

Year: 2009

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-009-2082-9

Paper Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast

Aza, Pablo; Molpeceres, Gonzalo; Salas, Felipe de et al. · 2021 · Cellular and Molecular Life Sciences · DOI

Full title: Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast

Authors: Aza, Pablo; Molpeceres, Gonzalo; Salas, Felipe de; Camarero, Susana

Year: 2021

Venue: Cellular and Molecular Life Sciences

DOI: https://doi.org/10.1007/s00018-021-03793-y

Paper Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris

Sygmund, Christoph; Gutmann, Alexander; Krondorfer, Iris et al. · 2011 · Applied Microbiology and Biotechnology · DOI

Full title: Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris

Authors: Sygmund, Christoph; Gutmann, Alexander; Krondorfer, Iris; Kujawa, Magdalena; Glieder, Anton; Pscheidt, Beate; Haltrich, Dietmar; Peterbauer, Clemens; Kittl, Roman

Year: 2011

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-011-3667-7

Paper An updated view on horseradish peroxidases: recombinant production and biotechnological applications

Krainer, Florian; Glieder, Anton · 2015 · Applied Microbiology and Biotechnology · DOI

Full title: An updated view on horseradish peroxidases: recombinant production and biotechnological applications

Authors: Krainer, Florian; Glieder, Anton

Year: 2015

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-014-6346-7

Paper Recombinant Aspergillus β-galactosidases as a robust glycomic and biotechnological tool

Dragosits, Martin; Pflügl, Stefan; Kurz, Simone et al. · 2013 · Applied Microbiology and Biotechnology · DOI

Full title: Recombinant Aspergillus β-galactosidases as a robust glycomic and biotechnological tool

Authors: Dragosits, Martin; Pflügl, Stefan; Kurz, Simone; Razzazi‐Fazeli, Ebrahim; Wilson, Iain B. H.; Rendić, Dubravko

Year: 2013

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-013-5192-3

Paper A homologous production system for Trichoderma reesei secreted proteins in a cellulase-free background

Uzbaş, Fatma; Sezerman, Osman Uğur; Hartl, Lukas et al. · 2011 · Applied Microbiology and Biotechnology · DOI

Full title: A homologous production system for Trichoderma reesei secreted proteins in a cellulase-free background

Authors: Uzbaş, Fatma; Sezerman, Osman Uğur; Hartl, Lukas; Kubicek, Christian P.; Seiboth, Bernhard

Year: 2011

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-011-3674-8

Paper Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

Mudassar Ahmad; Melanie Hirz; H. Pichler et al. · 2014 · Applied Microbiology and Biotechnology · DOI

Full title: Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

Authors: Mudassar Ahmad; Melanie Hirz; H. Pichler; H. Schwab

Year: 2014

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-014-5732-5

Paper Process development and scale-up optimization of the SARS-CoV-2 receptor binding domain–based vaccine candidate, RBD219-N1C1

Lee, Jungsoon; Liu, Zhuyun; Chen, Wen‐Hsiang et al. · 2021 · Applied Microbiology and Biotechnology · DOI

Full title: Process development and scale-up optimization of the SARS-CoV-2 receptor binding domain–based vaccine candidate, RBD219-N1C1

Authors: Lee, Jungsoon; Liu, Zhuyun; Chen, Wen‐Hsiang; Wei, Junfei; Kundu, Rakhi; Adhikari, Rakesh; Rivera, Joanne Altieri; Gillespie, Portia M.; Strych, Ulrich; Zhan, Bin; Hotez, Peter J.; Bottazzi, María Elena

Year: 2021

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-021-11281-3

Paper Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfolded proteins using chemostat cultures

Hesketh, Andrew; Castrillo, Juan I.; Sawyer, Trevor et al. · 2013 · Applied Microbiology and Biotechnology · DOI

Full title: Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfolded proteins using chemostat cultures

Authors: Hesketh, Andrew; Castrillo, Juan I.; Sawyer, Trevor; Archer, David B.; Oliver, Stephen G.

Year: 2013

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-013-5186-1

Paper Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter

Looser, Verena; Lüthy, Dominik; Straumann, Marcel et al. · 2017 · Applied Microbiology and Biotechnology · DOI

Full title: Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter

Authors: Looser, Verena; Lüthy, Dominik; Straumann, Marcel; Hecht, Katrin; Melzoch, Karel; Kovar, Karin

Year: 2017

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-017-8123-x

Paper Filamentous fungi-like secretory pathway strayed in a yeast system: peculiarities of Yarrowia lipolytica secretory pathway underlying its extraordinary performance

Celińska, Ewelina; Nicaud, Jean‐Marc · 2018 · Applied Microbiology and Biotechnology · DOI

Full title: Filamentous fungi-like secretory pathway strayed in a yeast system: peculiarities of Yarrowia lipolytica secretory pathway underlying its extraordinary performance

Authors: Celińska, Ewelina; Nicaud, Jean‐Marc

Year: 2018

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-018-9450-2

Paper Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris

Nocon, Justyna; Steiger, Matthias G.; Mairinger, Teresa et al. · 2016 · Applied Microbiology and Biotechnology · DOI

Full title: Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris

Authors: Nocon, Justyna; Steiger, Matthias G.; Mairinger, Teresa; Hohlweg, Jonas; Rußmayer, Hannes; Hann, Stephan; Gasser, Brigitte; Mattanovich, Diethard

Year: 2016

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-016-7363-5

Paper Robust signal peptides for protein secretion in Yarrowia lipolytica: identification and characterization of novel secretory tags

Celińska, Ewelina; Borkowska, Monika; Białas, W. et al. · 2018 · Applied Microbiology and Biotechnology · DOI

Full title: Robust signal peptides for protein secretion in Yarrowia lipolytica: identification and characterization of novel secretory tags

Authors: Celińska, Ewelina; Borkowska, Monika; Białas, W.; Korpys-Woźniak, Paulina; Nicaud, Jean‐Marc

Year: 2018

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-018-8966-9

Paper High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors

Jankowski, Nina; Koschorreck, Katja; Urlacher, Vlada B. · 2020 · Applied Microbiology and Biotechnology · DOI

Full title: High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors

Authors: Jankowski, Nina; Koschorreck, Katja; Urlacher, Vlada B.

Year: 2020

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-020-10878-4

Paper Pecularities and applications of aryl-alcohol oxidases from fungi

Urlacher, Vlada B.; Koschorreck, Katja · 2021 · Applied Microbiology and Biotechnology · DOI

Full title: Pecularities and applications of aryl-alcohol oxidases from fungi

Authors: Urlacher, Vlada B.; Koschorreck, Katja

Year: 2021

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-021-11337-4

Paper Production and secretion dynamics of prokaryotic Penicillin G acylase in Pichia pastoris.

Borčinová, Martina; Raschmanová, Hana; Zamora, Iwo et al. · 2020 · Applied microbiology and biotechnology · DOI

Full title: Production and secretion dynamics of prokaryotic Penicillin G acylase in Pichia pastoris.

Authors: Borčinová, Martina; Raschmanová, Hana; Zamora, Iwo; Looser, Verena; Marešová, Helena; Hirsch, Sven; Kyslík, Pavel; Kovar, Karin

Year: 2020

Venue: Applied microbiology and biotechnology

DOI: https://doi.org/10.1007/s00253-020-10669-x

Paper Novel in vitro inhibitory functions of potato tuber proteinaceous inhibitors

Fischer, Matthias; Kuckenberg, Markus; Kastilan, Robin et al. · 2014 · Molecular Genetics and Genomics · DOI

Full title: Novel in vitro inhibitory functions of potato tuber proteinaceous inhibitors

Authors: Fischer, Matthias; Kuckenberg, Markus; Kastilan, Robin; Muth, Jost; Gebhardt, Christiane

Year: 2014

Venue: Molecular Genetics and Genomics

DOI: https://doi.org/10.1007/s00438-014-0906-5

Paper Real-time estimation of biomass and specific growth rate in physiologically variable recombinant fed-batch processes

Wechselberger, Patrick; Sagmeister, Patrick; Herwig, Christoph · 2012 · Bioprocess and Biosystems Engineering · DOI

Full title: Real-time estimation of biomass and specific growth rate in physiologically variable recombinant fed-batch processes

Authors: Wechselberger, Patrick; Sagmeister, Patrick; Herwig, Christoph

Year: 2012

Venue: Bioprocess and Biosystems Engineering

DOI: https://doi.org/10.1007/s00449-012-0848-4

Paper Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins.

Raschmanová, Hana; Weninger, Astrid; Knejzlík, Zdeněk et al. · 2021 · Applied microbiology and biotechnology · DOI

Full title: Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins.

Authors: Raschmanová, Hana; Weninger, Astrid; Knejzlík, Zdeněk; Melzoch, Karel; Kovar, Karin

Year: 2021

Venue: Applied microbiology and biotechnology

DOI: https://doi.org/10.1007/s00253-021-11336-5

Paper Engineering protein translocation and unfolded protein response enhanced human PH-20 secretion in Pichia pastoris

Yue-Sheng Zhang; Jin-Song Gong; Jia-Yu Jiang et al. · 2024 · Applied Microbiology and Biotechnology · DOI

Full title: Engineering protein translocation and unfolded protein response enhanced human PH-20 secretion in Pichia pastoris

Authors: Yue-Sheng Zhang; Jin-Song Gong; Jia-Yu Jiang; Zhenghong Xu; Jin-song Shi

Year: 2024

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-023-12878-6

Paper A visual method for direct selection of high-producing Pichia pastorisclones

Hu, Fan; Li, Xin; Lü, Jie et al. · 2011 · BMC Biotechnology · DOI

Full title: A visual method for direct selection of high-producing Pichia pastorisclones

Authors: Hu, Fan; Li, Xin; Lü, Jie; Mao, P.-H.; Jin, Xiang; Rao, Ben; Zheng, Peng; Zhou, Yu; Liu, Sheng Yi; Ke, Tao; Dong, Xiang; Li, Xin

Year: 2011

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/1472-6750-11-23

Paper Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris

Nordén, Kristina; Agemark, Maria; Danielson, Jonas ÅH et al. · 2011 · BMC Biotechnology · DOI

Full title: Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris

Authors: Nordén, Kristina; Agemark, Maria; Danielson, Jonas ÅH; Alexandersson, Erik; Kjellbom, Per; Johanson, Urban

Year: 2011

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/1472-6750-11-47

Paper A new cold-adapted β-D-galactosidase from the Antarctic Arthrobacter sp. 32c – gene cloning, overexpression, purification and properties

Hildebrandt, Piotr; Wanarska, Marta; Kur, Józef · 2009 · BMC Microbiology · DOI

Full title: A new cold-adapted β-D-galactosidase from the Antarctic Arthrobacter sp. 32c – gene cloning, overexpression, purification and properties

Authors: Hildebrandt, Piotr; Wanarska, Marta; Kur, Józef

Year: 2009

Venue: BMC Microbiology

DOI: https://doi.org/10.1186/1471-2180-9-151

Paper Single chain Fab (scFab) fragment

Hust, Michael; Jostock, Thomas; Menzel, Christian et al. · 2007 · BMC Biotechnology · DOI

Full title: Single chain Fab (scFab) fragment

Authors: Hust, Michael; Jostock, Thomas; Menzel, Christian; Voedisch, Bernd; Mohr, Anja; Brenneis, Mariam; Kirsch, Martina Inga; Meier, Doris; Dübel, Stefan

Year: 2007

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/1472-6750-7-14

Paper The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis

Baumann, Kristin; Dato, Laura; Graf, Alexandra B. et al. · 2011 · BMC Genomics · DOI

Full title: The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis

Authors: Baumann, Kristin; Dato, Laura; Graf, Alexandra B.; Frascotti, Gianni; Dragosits, Martin; Porro, Danilo; Mattanovich, Diethard; Ferrer, Pau; Branduardi, Paola

Year: 2011

Venue: BMC Genomics

DOI: https://doi.org/10.1186/1471-2164-12-218

Paper Functional expression of a blood tolerant laccase in Pichia pastoris

D. Maté; David Gonzalez-Perez; R. Kittl et al. · 2013 · BMC Biotechnology · DOI

Full title: Functional expression of a blood tolerant laccase in Pichia pastoris

Authors: D. Maté; David Gonzalez-Perez; R. Kittl; R. Ludwig; M. Alcalde

Year: 2013

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/1472-6750-13-38

Paper Cloning, sequence analysis, expression of Cyathus bulleri laccase in Pichia pastoris and characterization of recombinant laccase

Garg, Neha; Bieler, Nora; Kenzom, T. et al. · 2012 · BMC Biotechnology · DOI

Full title: Cloning, sequence analysis, expression of Cyathus bulleri laccase in Pichia pastoris and characterization of recombinant laccase

Authors: Garg, Neha; Bieler, Nora; Kenzom, T.; Chhabra, Meenu; Ansorge‐Schumacher, Marion B.; Mishra, Saroj

Year: 2012

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/1472-6750-12-75

Paper The alkaline pectate lyase PEL168 of Bacillus subtilis heterologously expressed in Pichia pastorisis more stable and efficient for degumming ramie fiber

Zhang, Chengjie; Yao, Jia; Zhou, Cheng et al. · 2013 · BMC Biotechnology · DOI

Full title: The alkaline pectate lyase PEL168 of Bacillus subtilis heterologously expressed in Pichia pastorisis more stable and efficient for degumming ramie fiber

Authors: Zhang, Chengjie; Yao, Jia; Zhou, Cheng; Mao, Liangwei; Zhang, Guimin; Ma, Yanhe

Year: 2013

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/1472-6750-13-26

Paper Improved production of recombinant human Fas ligand extracellular domain in Pichia pastoris: yield enhancement using disposable culture-bag and its application to site-specific chemical modifications

M. Muraki · 2014 · BMC Biotechnology · DOI

Full title: Improved production of recombinant human Fas ligand extracellular domain in Pichia pastoris: yield enhancement using disposable culture-bag and its application to site-specific chemical modifications

Authors: M. Muraki

Year: 2014

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/1472-6750-14-19

Paper Systematic screening of glycosylation- and trafficking-associated gene knockouts in Saccharomyces cerevisiaeidentifies mutants with improved heterologous exocellulase activity and host secretion

Wang, Tzi‐Yuan; Huang, Chih-Jen; Chen, Hsin‐Liang et al. · 2013 · BMC Biotechnology · DOI

Full title: Systematic screening of glycosylation- and trafficking-associated gene knockouts in Saccharomyces cerevisiaeidentifies mutants with improved heterologous exocellulase activity and host secretion

Authors: Wang, Tzi‐Yuan; Huang, Chih-Jen; Chen, Hsin‐Liang; Ho, Po-Chun; Ke, Huei‐Mien; Cho, Hsing‐Yi; Ruan, Sz‐Kai; Hung, Kuo-Yen; Wang, I-Li; Cai, Ya-Wun; Sung, Huang‐Mo; Li, Wen‐Hsiung; Shih, Ming‐Che

Year: 2013

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/1472-6750-13-71

Paper Efficient production of human bivalent and trivalent anti-MUC1 Fab-scFv antibodies in Pichia pastoris

Schoonooghe, Steve; Kaigorodov, V. A.; Zawisza, Monika et al. · 2009 · BMC Biotechnology · DOI

Full title: Efficient production of human bivalent and trivalent anti-MUC1 Fab-scFv antibodies in Pichia pastoris

Authors: Schoonooghe, Steve; Kaigorodov, V. A.; Zawisza, Monika; Dumolyn, Caroline; Haustraete, Jurgen; Grooten, Johan; Mertens, Nico

Year: 2009

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/1472-6750-9-70

Paper A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris

Dietzsch, Christian; Spadiut, Oliver; Herwig, Christoph · 2011 · Microbial Cell Factories · DOI

Full title: A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris

Authors: Dietzsch, Christian; Spadiut, Oliver; Herwig, Christoph

Year: 2011

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-10-14

Paper A thermostable GH45 endoglucanase from yeast: impact of its atypical multimodularity on activity

Couturier, Marie; Féliu, Julia; Haon, Mireille et al. · 2011 · Microbial Cell Factories · DOI

Full title: A thermostable GH45 endoglucanase from yeast: impact of its atypical multimodularity on activity

Authors: Couturier, Marie; Féliu, Julia; Haon, Mireille; Navarro, David; Lesage‐Meessen, Laurence; Coutinho, Pedro M.; Berrin, Jean‐Guy

Year: 2011

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-10-103

Paper Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes

Bey, Mathieu; Berrin, Jean‐Guy; Poidevin, Laetitia et al. · 2011 · Microbial Cell Factories · DOI

Full title: Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes

Authors: Bey, Mathieu; Berrin, Jean‐Guy; Poidevin, Laetitia; Sigoillot, Jean‐Claude

Year: 2011

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-10-113

Paper Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry

Chan, Sam Wei Polly; Greaves, John; Silva, Nancy A. Da et al. · 2012 · BMC Biotechnology · DOI

Full title: Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry

Authors: Chan, Sam Wei Polly; Greaves, John; Silva, Nancy A. Da; Wang, Szu‐Wen

Year: 2012

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/1472-6750-12-51

Paper Heterologous overexpression of Glomerella cingulata FAD-dependent glucose dehydrogenase in Escherichia coli and Pichia pastoris

Sygmund, Christoph; Staudigl, Petra; Klausberger, Miriam et al. · 2011 · Microbial Cell Factories · DOI

Full title: Heterologous overexpression of Glomerella cingulata FAD-dependent glucose dehydrogenase in Escherichia coli and Pichia pastoris

Authors: Sygmund, Christoph; Staudigl, Petra; Klausberger, Miriam; Pinotsis, Nikos; Djinović‐Carugo, Kristina; Gorton, Lo; Haltrich, Dietmar; Ludwig, Roland

Year: 2011

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-10-106

Paper The response to unfolded protein is involved in osmotolerance of Pichia pastoris

Dragosits, Martin; Stadlmann, Johannes; Graf, Alexandra B. et al. · 2010 · BMC Genomics · DOI

Full title: The response to unfolded protein is involved in osmotolerance of Pichia pastoris

Authors: Dragosits, Martin; Stadlmann, Johannes; Graf, Alexandra B.; Gasser, Brigitte; Maurer, Michael; Sauer, Michael; Kreil, David P.; Altmann, Friedrich; Mattanovich, Diethard

Year: 2010

Venue: BMC Genomics

DOI: https://doi.org/10.1186/1471-2164-11-207

Paper Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris

Yu, Xiao‐Wei; Wang, Rui; Zhang, Meng et al. · 2012 · Microbial Cell Factories · DOI

Full title: Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris

Authors: Yu, Xiao‐Wei; Wang, Rui; Zhang, Meng; Xu, Yan; Xiao, Rong

Year: 2012

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-11-102

Paper A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains

Dietzsch, Christian; Spadiut, Oliver; Herwig, Christoph · 2011 · Microbial Cell Factories · DOI

Full title: A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains

Authors: Dietzsch, Christian; Spadiut, Oliver; Herwig, Christoph

Year: 2011

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-10-85

Paper Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments

Jafari, Rozbeh; Sundström, Birgitta; Holm, Patrik · 2011 · Microbial Cell Factories · DOI

Full title: Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments

Authors: Jafari, Rozbeh; Sundström, Birgitta; Holm, Patrik

Year: 2011

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-10-34

Paper Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen

Lünsdorf, Heinrich; Gurramkonda, Chandrasekhar; Adnan, Ahmad et al. · 2011 · Microbial Cell Factories · DOI

Full title: Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen

Authors: Lünsdorf, Heinrich; Gurramkonda, Chandrasekhar; Adnan, Ahmad; Khanna, Navin; Rinas, Ursula

Year: 2011

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-10-48

Paper Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedure

Pfeffer, Martin; Maurer, Michael; Köllensperger, Gunda et al. · 2011 · Microbial Cell Factories · DOI

Full title: Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedure

Authors: Pfeffer, Martin; Maurer, Michael; Köllensperger, Gunda; Hann, Stephan; Graf, Alexandra B.; Mattanovich, Diethard

Year: 2011

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-10-47

Paper Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris

Kristin Baumann; Núria Adelantado; C. Lang et al. · 2011 · Microbial Cell Factories · DOI

Full title: Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris

Authors: Kristin Baumann; Núria Adelantado; C. Lang; D. Mattanovich; P. Ferrer

Year: 2011

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-10-93

Paper Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield

Routledge, Sarah J; Hewitt, Christopher J.; Bora, Nagamani et al. · 2011 · Microbial Cell Factories · DOI

Full title: Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield

Authors: Routledge, Sarah J; Hewitt, Christopher J.; Bora, Nagamani; Bill, Roslyn M.

Year: 2011

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-10-17

Paper Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes

Vanz, AnaLeticia; Lünsdorf, Heinrich; Adnan, Ahmad et al. · 2012 · Microbial Cell Factories · DOI

Full title: Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes

Authors: Vanz, AnaLeticia; Lünsdorf, Heinrich; Adnan, Ahmad; Nimtz, Manfred; Gurramkonda, Chandrasekhar; Khanna, Navin; Rinas, Ursula

Year: 2012

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-11-103

Paper Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

Joel Jordà; P. Jouhten; Elena Cámara et al. · 2012 · Microbial Cell Factories · DOI

Full title: Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

Authors: Joel Jordà; P. Jouhten; Elena Cámara; H. Maaheimo; J. Albiol; P. Ferrer

Year: 2012

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-11-57

Paper Expression of lignocellulolytic enzymes in Pichia pastoris

A. Mellitzer; R. Weis; A. Glieder et al. · 2012 · Microbial Cell Factories · DOI

Full title: Expression of lignocellulolytic enzymes in Pichia pastoris

Authors: A. Mellitzer; R. Weis; A. Glieder; K. Flicker

Year: 2012

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-11-61

Paper Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33

Liu, Dongyang; Zhang, Ruifu; Yang, Xingming et al. · 2012 · Microbial Cell Factories · DOI

Full title: Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33

Authors: Liu, Dongyang; Zhang, Ruifu; Yang, Xingming; Zhang, Zhenhua; Song, Song; Miao, Youzhi; Shen, Qirong

Year: 2012

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-11-25

Paper Systems metabolic engineering, industrial biotechnology and microbial cell factories

Lee, Sang Yup; Mattanovich, Diethard; Villaverde, Antonio · 2012 · Microbial Cell Factories · DOI

Full title: Systems metabolic engineering, industrial biotechnology and microbial cell factories

Authors: Lee, Sang Yup; Mattanovich, Diethard; Villaverde, Antonio

Year: 2012

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-11-156

Paper Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions

Marc Carnicer; A. ten Pierick; J. V. van Dam et al. · 2012 · Microbial Cell Factories · DOI

Full title: Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions

Authors: Marc Carnicer; A. ten Pierick; J. V. van Dam; J. Heijnen; J. Albiol; W. V. van Gulik; P. Ferrer

Year: 2012

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-11-83

Paper Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

Shiroishi, Mitsunori; Tsujimoto, Hirokazu; Makyio, Hisayoshi et al. · 2012 · Microbial Cell Factories · DOI

Full title: Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

Authors: Shiroishi, Mitsunori; Tsujimoto, Hirokazu; Makyio, Hisayoshi; Asada, Hidetsugu; Yurugi-Kobayashi, Takami; Shimamura, Tatsuro; Murata, Takeshi; Nomura, Norimichi; Haga, Tatsuya; Iwata, So; Kobayashi, Takuya

Year: 2012

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-11-78

Paper Enhanced membrane protein expression by engineering increased intracellular membrane production

Guerfal, Mouna; Claes, Katrien; Knittelfelder, Oskar et al. · 2013 · Microbial Cell Factories · DOI

Full title: Enhanced membrane protein expression by engineering increased intracellular membrane production

Authors: Guerfal, Mouna; Claes, Katrien; Knittelfelder, Oskar; Rycke, Riet De; Kohlwein, Sepp D.; Callewaert, Nico

Year: 2013

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-12-122

Paper Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

Chen, Ming‐Tang; Lin, Song; Shandil, Ishaan et al. · 2012 · Microbial Cell Factories · DOI

Full title: Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

Authors: Chen, Ming‐Tang; Lin, Song; Shandil, Ishaan; Andrews, Dewan; Stadheim, Terrance A.; Choi, Byung‐Kwon

Year: 2012

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-11-91

Paper Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES.

Jariyachawalid, Kanidtha; Laowanapiban, Poramaet; Meevootisom, Vithaya et al. · 2012 · Microbial cell factories · DOI

Full title: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES.

Authors: Jariyachawalid, Kanidtha; Laowanapiban, Poramaet; Meevootisom, Vithaya; Wiyakrutta, Suthep

Year: 2012

Venue: Microbial cell factories

DOI: https://doi.org/10.1186/1475-2859-11-47

Paper Overexpression of an exotic thermotolerant β-glucosidase in trichoderma reesei and its significant increase in cellulolytic activity and saccharification of barley straw

Dashtban, Mehdi; Qin, Wensheng · 2012 · Microbial Cell Factories · DOI

Full title: Overexpression of an exotic thermotolerant β-glucosidase in trichoderma reesei and its significant increase in cellulolytic activity and saccharification of barley straw

Authors: Dashtban, Mehdi; Qin, Wensheng

Year: 2012

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-11-63

Paper Recombinant sterol esterase from Ophiostoma piceae: an improved biocatalyst expressed in Pichia pastoris

Cedillo, Víctor Barba; Plou, Francisco J.; Martı́nez, Marı́a Jesús · 2012 · Microbial Cell Factories · DOI

Full title: Recombinant sterol esterase from Ophiostoma piceae: an improved biocatalyst expressed in Pichia pastoris

Authors: Cedillo, Víctor Barba; Plou, Francisco J.; Martı́nez, Marı́a Jesús

Year: 2012

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-11-73

Paper Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

Prielhofer, Roland; Maurer, Michael; Klein, Joachim et al. · 2013 · Microbial Cell Factories · DOI

Full title: Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

Authors: Prielhofer, Roland; Maurer, Michael; Klein, Joachim; Wenger, Jana; Kiziak, Christoph; Gasser, Brigitte; Mattanovich, Diethard

Year: 2013

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-12-5

Paper Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures

Ana Letícia Vanz; M. Nimtz; U. Rinas · 2014 · Microbial Cell Factories · DOI

Full title: Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures

Authors: Ana Letícia Vanz; M. Nimtz; U. Rinas

Year: 2014

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-13-23

Paper Towards better understanding of an industrial cell factory: investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris

Fazenda, Mariana L.; Dias, João M.L.; Harvey, Linda M. et al. · 2013 · Microbial Cell Factories · DOI

Full title: Towards better understanding of an industrial cell factory: investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris

Authors: Fazenda, Mariana L.; Dias, João M.L.; Harvey, Linda M.; Nordon, Alison; Edrada‐Ebel, RuAngelie; Littlejohn, David; McNeil, Brian

Year: 2013

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-12-51

Paper Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

Gao, Limei; Cai, Menghao; Shen, Wei et al. · 2013 · Microbial Cell Factories · DOI

Full title: Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

Authors: Gao, Limei; Cai, Menghao; Shen, Wei; Xiao, Siwei; Zhou, Xiangshan; Zhang, Yuanxing

Year: 2013

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-12-77

Paper Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

J. Hemmerich; Núria Adelantado; J. M. Barrigón et al. · 2014 · Microbial Cell Factories · DOI

Full title: Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

Authors: J. Hemmerich; Núria Adelantado; J. M. Barrigón; Xavier Ponte; Astrid Hörmann; P. Ferrer; F. Kensy; F. Valero

Year: 2014

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-13-36

Paper A quantitative study of methanol/sorbitol co-feeding process of a Pichia pastoris Mut+/pAOX1-lacZ strain

Niu, Hongxing; Jost, Laurent; Pirlot, Nathalie et al. · 2013 · Microbial Cell Factories · DOI

Full title: A quantitative study of methanol/sorbitol co-feeding process of a Pichia pastoris Mut+/pAOX1-lacZ strain

Authors: Niu, Hongxing; Jost, Laurent; Pirlot, Nathalie; Sassi, Hosni; Daukandt, Marc; Rodriguez, Christian; Fickers, Patrick

Year: 2013

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-12-33

Paper Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production

Sygmund, Christoph; Santner, Paul; Krondorfer, Iris et al. · 2013 · Microbial Cell Factories · DOI

Full title: Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production

Authors: Sygmund, Christoph; Santner, Paul; Krondorfer, Iris; Peterbauer, Clemens; Alcalde, Miguel; Nyanhongo, Gibson S.; Guebitz, Georg M.; Ludwig, Roland

Year: 2013

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-12-38

Paper Yeast biotechnology: teaching the old dog new tricks

Mattanovich, Diethard; Sauer, Michael; Gasser, Brigitte · 2014 · Microbial Cell Factories · DOI

Full title: Yeast biotechnology: teaching the old dog new tricks

Authors: Mattanovich, Diethard; Sauer, Michael; Gasser, Brigitte

Year: 2014

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-13-34

Paper Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum

Matsuda, Yoshihiko; Itaya, Hiroshi; Kitahara, Yuki et al. · 2014 · Microbial Cell Factories · DOI

Full title: Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum

Authors: Matsuda, Yoshihiko; Itaya, Hiroshi; Kitahara, Yuki; Theresia, Natalia Maria; Kutukova, Ekaterina A.; Yomantas, Yurgis Antanas Vladovich; Date, Masayo; Kikuchi, Yoshimi; Wachi, Masaaki

Year: 2014

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-13-56

Paper Expression of endoglucanases in Pichia pastoris under control of the GAP promoter

Várnai, Anikó; Tang, Campbell; Bengtsson, Oskar et al. · 2014 · Microbial Cell Factories · DOI

Full title: Expression of endoglucanases in Pichia pastoris under control of the GAP promoter

Authors: Várnai, Anikó; Tang, Campbell; Bengtsson, Oskar; Atterton, Andrew; Mathiesen, Geir; Eijsink, Vincent G. H.

Year: 2014

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-13-57

Paper Carbon source dependent promoters in yeasts

Weinhandl, Katrin; Winkler, Margit; Glieder, Anton et al. · 2014 · Microbial Cell Factories · DOI

Full title: Carbon source dependent promoters in yeasts

Authors: Weinhandl, Katrin; Winkler, Margit; Glieder, Anton; Camattari, Andrea

Year: 2014

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-13-5

Paper Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

Sauer, Michael; Branduardi, Paola; Gasser, Brigitte et al. · 2004 · Microbial Cell Factories · DOI

Full title: Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

Authors: Sauer, Michael; Branduardi, Paola; Gasser, Brigitte; Valli, Minoska; Maurer, Michael; Porro, Danilo; Mattanovich, Diethard

Year: 2004

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-3-17

Paper Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris

Gürkan, Cemal; Ellar, David J. · 2005 · Microbial Cell Factories · DOI

Full title: Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris

Authors: Gürkan, Cemal; Ellar, David J.

Year: 2005

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-4-33

Paper Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review

Cós, Oriol; Ramón, Ramón; Montesinos, José Luis González et al. · 2006 · Microbial Cell Factories · DOI

Full title: Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review

Authors: Cós, Oriol; Ramón, Ramón; Montesinos, José Luis González; Valero, Francisco

Year: 2006

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-5-17

Paper The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi

Joosten, Vivi; Lokman, Christien; Hondel, Cees A. M. J. J. van den et al. · 2003 · Microbial Cell Factories · DOI

Full title: The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi

Authors: Joosten, Vivi; Lokman, Christien; Hondel, Cees A. M. J. J. van den; Punt, Peter J.

Year: 2003

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-2-1

Paper Regulation of methanol utilisation pathway genes in yeasts.

Hartner, Franz Stefan; Glieder, Anton · 2006 · Microbial Cell Factories · DOI

Full title: Regulation of methanol utilisation pathway genes in yeasts.

Authors: Hartner, Franz Stefan; Glieder, Anton

Year: 2006

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-5-39

Paper Application of a wide-range yeast vector (CoMed™) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts

Steinborn, Gerhard; Böer, Erik; Scholz, Anja et al. · 2006 · Microbial Cell Factories · DOI

Full title: Application of a wide-range yeast vector (CoMed™) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts

Authors: Steinborn, Gerhard; Böer, Erik; Scholz, Anja; Tag, Kristina; Kunze, Gotthard; Gellissen, Gerd

Year: 2006

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-5-33

Paper Heterologous expression of lcc1 gene from Trametes trogii in Pichia pastoris and characterization of the recombinant enzyme.

Colao, M. Ch.; Lupino, Stefania; Garzillo, Anna Maria Vittoria et al. · 2006 · Microbial Cell Factories · DOI

Full title: Heterologous expression of lcc1 gene from Trametes trogii in Pichia pastoris and characterization of the recombinant enzyme.

Authors: Colao, M. Ch.; Lupino, Stefania; Garzillo, Anna Maria Vittoria; Buonocore, Vincenzo; Ruzzi, Maurizio

Year: 2006

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-5-31

Paper Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris

Maurer, Michael; Kühleitner, Manfred; Gasser, Brigitte et al. · 2006 · Microbial Cell Factories · DOI

Full title: Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris

Authors: Maurer, Michael; Kühleitner, Manfred; Gasser, Brigitte; Mattanovich, Diethard

Year: 2006

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-5-37

Paper Role of cultivation media in the development of yeast strains for large scale industrial use

Hahn‐Hägerdal, Bärbel; Karhumaa, Kaisa; Larsson, Christer et al. · 2005 · Microbial Cell Factories · DOI

Full title: Role of cultivation media in the development of yeast strains for large scale industrial use

Authors: Hahn‐Hägerdal, Bärbel; Karhumaa, Kaisa; Larsson, Christer; Gorwa‐Grauslund, Marie F.; Görgens, Johann F.; Zyl, Willem H. van

Year: 2005

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-4-31

Paper Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris

Surribas, Anna; Resina, David; Ferrer, Pau et al. · 2007 · Microbial Cell Factories · DOI

Full title: Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris

Authors: Surribas, Anna; Resina, David; Ferrer, Pau; Valero, Francisco

Year: 2007

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-6-15

Paper Transcriptional analysis of protein production and induction of unfolded protein response in Pichia pastoris expressing a Rhizopus oryzae lipase under the FLD1 promoter

David Resina; M. Bollók; F. Valero et al. · 2006 · DOI

Full title: Transcriptional analysis of protein production and induction of unfolded protein response in Pichia pastoris expressing a Rhizopus oryzae lipase under the FLD1 promoter

Authors: David Resina; M. Bollók; F. Valero; P. Neubauer; P. Ferrer

Year: 2006

DOI: https://doi.org/10.1186/1475-2859-5-s1-p49

Paper Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

Gasser, Brigitte; Saloheimo, Markku; Rinas, Ursula et al. · 2008 · Microbial Cell Factories · DOI

Full title: Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

Authors: Gasser, Brigitte; Saloheimo, Markku; Rinas, Ursula; Dragosits, Martin; Rodríguez‐Carmona, Escarlata; Baumann, Kristin; Giuliani, Maria Elisa; Parrilli, Ermenegilda; Branduardi, Paola; Lang, Christine; Porro, Danilo; Ferrer, Pau; Tutino, Maria Luisa; Mattanovich, Diethard; Villaverde, Antonio

Year: 2008

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-7-11

Paper Engineering and Applications of fungal laccases for organic synthesis

Kunamneni, Adinarayana; Camarero, Susana; García-Burgos, Carlos et al. · 2008 · Microbial Cell Factories · DOI

Full title: Engineering and Applications of fungal laccases for organic synthesis

Authors: Kunamneni, Adinarayana; Camarero, Susana; García-Burgos, Carlos; Plou, Francisco J.; Ballesteros, Antonio; Alcalde, Miguel

Year: 2008

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-7-32

Paper Substrate feeding strategies in Pichia pastoris fed-batch cultivation processes: Analysis of key parameters influencing recombinant protein production

Ramón, Ramón; Cós, Oriol; Ferrer, Pau et al. · 2006 · Microbial Cell Factories · DOI

Full title: Substrate feeding strategies in Pichia pastoris fed-batch cultivation processes: Analysis of key parameters influencing recombinant protein production

Authors: Ramón, Ramón; Cós, Oriol; Ferrer, Pau; Montesinos, José Luis González; Valero, Francisco

Year: 2006

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-5-s1-s13

Paper Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

Mattanovich, Diethard; Graf, Alexandra B.; Stadlmann, Johannes et al. · 2009 · Microbial Cell Factories · DOI

Full title: Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

Authors: Mattanovich, Diethard; Graf, Alexandra B.; Stadlmann, Johannes; Dragosits, Martin; Redl, Andréas; Maurer, Michael; Kleinheinz, Martin; Sauer, Michael; Altmann, Friedrich; Gasser, Brigitte

Year: 2009

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-8-29

Paper Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

Hans Marx; D. Mattanovich; M. Sauer · 2008 · Microbial Cell Factories · DOI

Full title: Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

Authors: Hans Marx; D. Mattanovich; M. Sauer

Year: 2008

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-7-23

Paper Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase

Rodríguez, Ángel Pereira; Fernández-Leiro, Rafael; Trillo, M Cristina et al. · 2006 · Microbial Cell Factories · DOI

Full title: Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase

Authors: Rodríguez, Ángel Pereira; Fernández-Leiro, Rafael; Trillo, M Cristina; Cerdán, M. Esperanza; González-Siso, María-Isabel; Becerra, Manuel

Year: 2006

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-5-41

Paper Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels

Carnicer, Marc; Baumann, Kristin; Töplitz, Isabelle et al. · 2009 · Microbial Cell Factories · DOI

Full title: Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels

Authors: Carnicer, Marc; Baumann, Kristin; Töplitz, Isabelle; Sánchez-Ferrando, Francesc; Mattanovich, Diethard; Ferrer, Pau; Albiol, Joan

Year: 2009

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-8-65

Paper Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

Bonander, Nicklas; Darby, Richard A. J.; Grgic, Ljuban et al. · 2009 · Microbial Cell Factories · DOI

Full title: Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

Authors: Bonander, Nicklas; Darby, Richard A. J.; Grgic, Ljuban; Bora, Nagamani; Wen, Jikai; Brogna, Saverio; Poyner, David R.; O’Neill, Michael; Bill, Roslyn M.

Year: 2009

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-8-10

Paper Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction.

Resina, David; Bollók, Mónika; Khatri, Narendar K et al. · 2007 · Microbial cell factories · DOI

Full title: Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction.

Authors: Resina, David; Bollók, Mónika; Khatri, Narendar K; Valero, Francisco; Neubauer, Peter; Ferrer, Pau

Year: 2007

Venue: Microbial cell factories

DOI: https://doi.org/10.1186/1475-2859-6-21

Paper Microbial factories for recombinant pharmaceuticals

Ferrer‐Miralles, Neus; Domingo‐Espín, Joan; Corchero, José Luís et al. · 2009 · Microbial Cell Factories · DOI

Full title: Microbial factories for recombinant pharmaceuticals

Authors: Ferrer‐Miralles, Neus; Domingo‐Espín, Joan; Corchero, José Luís; Vázquez, Esther; Villaverde, Antonio

Year: 2009

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-8-17

Paper Yeast cell factory: fishing for the best one or engineering it?

Porro, Danilo; Branduardi, Paola · 2009 · Microbial Cell Factories · DOI

Full title: Yeast cell factory: fishing for the best one or engineering it?

Authors: Porro, Danilo; Branduardi, Paola

Year: 2009

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-8-51

Paper Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

Krainer, Florian; Dietzsch, Christian; Hajek, Tanja et al. · 2012 · Microbial Cell Factories · DOI

Full title: Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

Authors: Krainer, Florian; Dietzsch, Christian; Hajek, Tanja; Herwig, Christoph; Spadiut, Oliver; Glieder, Anton

Year: 2012

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-11-22

Paper Open access to sequence: Browsing the Pichia pastoris genome

Mattanovich, Diethard; Callewaert, Nico; Rouzé, Pierre et al. · 2009 · Microbial Cell Factories · DOI

Full title: Open access to sequence: Browsing the Pichia pastoris genome

Authors: Mattanovich, Diethard; Callewaert, Nico; Rouzé, Pierre; Lin, Yao‐Cheng; Graf, Alexandra B.; Redl, Andréas; Tiels, Petra; Gasser, Brigitte; Schutter, Kristof De

Year: 2009

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-8-53

Paper Tuning microbial hosts for membrane protein production

Freigaßner, Maria Elisabeth; Pichler, Harald; Glieder, Anton · 2009 · Microbial Cell Factories · DOI

Full title: Tuning microbial hosts for membrane protein production

Authors: Freigaßner, Maria Elisabeth; Pichler, Harald; Glieder, Anton

Year: 2009

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-8-69

Paper The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

Guerfal, Mouna; Ryckaert, Stefan; Jacobs, Pieter P. et al. · 2010 · Microbial Cell Factories · DOI

Full title: The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

Authors: Guerfal, Mouna; Ryckaert, Stefan; Jacobs, Pieter P.; Ameloot, Paul; Craenenbroeck, Kathleen Van; Derycke, Riet; Callewaert, Nico

Year: 2010

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-9-49

Paper Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst

Abad, Sandra; Nahálka, Jozef; Bergler, Gabriele et al. · 2010 · Microbial Cell Factories · DOI

Full title: Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst

Authors: Abad, Sandra; Nahálka, Jozef; Bergler, Gabriele; Arnold, S. Alison; Speight, Robert; Fotheringham, Ian; Nidetzky, Bernd; Glieder, Anton

Year: 2010

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-9-24

Paper Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands

K. Ettayebi; M. Hardy · 2008 · Virology Journal · DOI

Full title: Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands

Authors: K. Ettayebi; M. Hardy

Year: 2008

Venue: Virology Journal

DOI: https://doi.org/10.1186/1743-422x-5-21

Paper Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence

Bai, Yingguo; Wang, Jian‐She; Zhang, Zhifang et al. · 2010 · Microbial Cell Factories · DOI

Full title: Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence

Authors: Bai, Yingguo; Wang, Jian‐She; Zhang, Zhifang; Shi, Pengjun; Luo, Huiying; Huang, Huoqing; Luo, Chunliang; Yao, Bin

Year: 2010

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-9-33

Paper High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture

Chen, Ruidong; Zhou, Zhigang; Cao, Yanan et al. · 2010 · Microbial Cell Factories · DOI

Full title: High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture

Authors: Chen, Ruidong; Zhou, Zhigang; Cao, Yanan; Bai, Yingguo; Yao, Bin

Year: 2010

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-9-39

Paper Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement

Bevan Ks Chung; Suresh Selvarasu; Camattari Andrea et al. · 2010 · Microbial Cell Factories · DOI

Full title: Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement

Authors: Bevan Ks Chung; Suresh Selvarasu; Camattari Andrea; Jimyoung Ryu; H. Lee; Jungoh Ahn; Hongweon Lee; Dong-Yup Lee

Year: 2010

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-9-50

Paper High yield expression of leptospirosis vaccine candidates LigA and LipL32 in the methylotrophic yeast Pichia pastoris

Hartwig, Daiane Drawanz; Oliveira, Thaís Larré; Seixas, Fabiana K. et al. · 2010 · Microbial Cell Factories · DOI

Full title: High yield expression of leptospirosis vaccine candidates LigA and LipL32 in the methylotrophic yeast Pichia pastoris

Authors: Hartwig, Daiane Drawanz; Oliveira, Thaís Larré; Seixas, Fabiana K.; Forster, Karine Maciel; Rizzi, Caroline; Hartleben, Cláudia Pinho; McBride, Alan J. A.; Dellagostin, Odir Antônio

Year: 2010

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-9-98

Paper Engineering Yarrowia lipolytica to Produce Glycoproteins Homogeneously Modified with the Universal Man3GlcNAc2 N-Glycan Core

Pourcq, Karen De; Tiels, Petra; Hecke, Annelies Van et al. · 2012 · PLoS ONE · DOI

Full title: Engineering Yarrowia lipolytica to Produce Glycoproteins Homogeneously Modified with the Universal Man3GlcNAc2 N-Glycan Core

Authors: Pourcq, Karen De; Tiels, Petra; Hecke, Annelies Van; Geysens, Steven; Vervecken, Wouter; Callewaert, Nico

Year: 2012

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0039976

Paper Implications of biodiesel production for enhancing thermostable T1.2RQ lipase secretion in Pichia pastoris via chaperone co-expression

E. Puspitasari; Y. Rustam; Andreas Adhi Satya et al. · 2025 · Biodiversitas Journal of Biological Diversity · DOI

Full title: Implications of biodiesel production for enhancing thermostable T1.2RQ lipase secretion in Pichia pastoris via chaperone co-expression

Authors: E. Puspitasari; Y. Rustam; Andreas Adhi Satya; Antonius Suwanto; A. Wahyudi; R. Astuti

Year: 2025

Venue: Biodiversitas Journal of Biological Diversity

DOI: https://doi.org/10.13057/biodiv/d260927

Paper Codon Optimization Significantly Improves the Expression Level of a Keratinase Gene in Pichia pastoris

Hu, Hong; Gao, Jie; He, Jun et al. · 2013 · PLoS ONE · DOI

Full title: Codon Optimization Significantly Improves the Expression Level of a Keratinase Gene in Pichia pastoris

Authors: Hu, Hong; Gao, Jie; He, Jun; Yu, Bing; Zheng, Ping; Huang, Zhiqing; Mao, Xiangbing; Yu, Jie; Han, Guoquan; Chen, Daiwen

Year: 2013

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0058393

Paper Recombinant Expression of Margatoxin and Agitoxin-2 in Pichia pastoris: An Efficient Method for Production of KV1.3 Channel Blockers

Anangi, Raveendra; Koshy, Shyny; Huq, Redwan et al. · 2012 · PLoS ONE · DOI

Full title: Recombinant Expression of Margatoxin and Agitoxin-2 in Pichia pastoris: An Efficient Method for Production of KV1.3 Channel Blockers

Authors: Anangi, Raveendra; Koshy, Shyny; Huq, Redwan; Beeton, Christine; Chuang, Woei‐Jer; King, Glenn F.

Year: 2012

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0052965

Paper A Dual-Mode Surface Display System for the Maturation and Production of Monoclonal Antibodies in Glyco-Engineered Pichia pastoris

Shaheen, Hussam; Prinz, Bianka; Chen, Ming‐Tang et al. · 2013 · PLoS ONE · DOI

Full title: A Dual-Mode Surface Display System for the Maturation and Production of Monoclonal Antibodies in Glyco-Engineered Pichia pastoris

Authors: Shaheen, Hussam; Prinz, Bianka; Chen, Ming‐Tang; Pavoor, Tej Venkatachalam; Lin, Song; Houston-Cummings, Nga Rewa; Moore, Renée; Stadheim, Terrance A.; Zha, Dongxing

Year: 2013

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0070190

Paper β-Glucan Synthase Gene Overexpression and β-Glucans Overproduction in Pleurotus ostreatus Using Promoter Swapping

Chai, Ran; Qiu, Cuiwei; DongRen, Liu et al. · 2013 · PLoS ONE · DOI

Full title: β-Glucan Synthase Gene Overexpression and β-Glucans Overproduction in Pleurotus ostreatus Using Promoter Swapping

Authors: Chai, Ran; Qiu, Cuiwei; DongRen, Liu; Qi, Yuancheng; Gao, Yuqian; Shen, Jinwen; Qiu, Liyou

Year: 2013

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0061693

Paper de novo Design and Synthesis of Candida antarctica Lipase B Gene and α-Factor Leads to High-Level Expression in Pichia pastoris

Yang, Jiangke; Liu, Liying; Dai, Jianghong et al. · 2013 · PLoS ONE · DOI

Full title: de novo Design and Synthesis of Candida antarctica Lipase B Gene and α-Factor Leads to High-Level Expression in Pichia pastoris

Authors: Yang, Jiangke; Liu, Liying; Dai, Jianghong; Li, Qin

Year: 2013

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0053939

Paper Conformational and Thermal Stability Improvements for the Large-Scale Production of Yeast-Derived Rabbit Hemorrhagic Disease Virus-Like Particles as Multipurpose Vaccine

Fernández, Erlinda; Toledo, Jorge R.; Méndez, Lídice et al. · 2013 · PLoS ONE · DOI

Full title: Conformational and Thermal Stability Improvements for the Large-Scale Production of Yeast-Derived Rabbit Hemorrhagic Disease Virus-Like Particles as Multipurpose Vaccine

Authors: Fernández, Erlinda; Toledo, Jorge R.; Méndez, Lídice; Gonzalez, N Lastres; Parra, Francisco; Martı́n-Alonso, José M.; Limonta‐Fernández, Miladys; Sánchez, Kosara; Cabrales, Ania; Estrada, Mario Pablo; Rodríguez-Mallón, Alina; Farnós, Omar

Year: 2013

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0056417

Paper Characterization of the Pichia pastoris Protein-O-mannosyltransferase Gene Family

Nett, Juergen H.; Cook, W. James; Mingtang, Chen et al. · 2013 · PLoS ONE · DOI

Full title: Characterization of the Pichia pastoris Protein-O-mannosyltransferase Gene Family

Authors: Nett, Juergen H.; Cook, W. James; Mingtang, Chen; Davidson, Robert C.; Bobrowicz, Piotr; Kett, Warren C.; Brevnova, Elena E.; Potgieter, Thomas I.; Mellon, Mark T.; Prinz, Bianka; Choi, Byung‐Kwon; Zha, Dongxing; Burnina, Irina; Bukowski, John; Du, ‬Min; Wildt, Stefan; Hamilton, Stephen R.

Year: 2013

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0068325

Paper Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site

Song, Yang; Kuang, Ye; Li, Hongbo et al. · 2013 · PLoS ONE · DOI

Full title: Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site

Authors: Song, Yang; Kuang, Ye; Li, Hongbo; Liu, Yue-Hong; Hui, Xiaoyan; Li, Peng; Jiang, Zhiwu; Zhou, Yulai; Wang, Yu; Xu, Aimin; Li, Shiwu; Liu, Pentao; Wu, Donghai

Year: 2013

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0075347

Paper Rational Design of a Fibroblast Growth Factor 21-Based Clinical Candidate, LY2405319

Kharitonenkov, Alexei; Beals, John M.; Micanovic, Radmila et al. · 2013 · PLoS ONE · DOI

Full title: Rational Design of a Fibroblast Growth Factor 21-Based Clinical Candidate, LY2405319

Authors: Kharitonenkov, Alexei; Beals, John M.; Micanovic, Radmila; Strifler, Beth A.; Rathnachalam, Radhakrishnan; Wroblewski, Victor J.; Li, Shun; Köester, Anja; Ford, Amy; Coşkun, Tamer; Dunbar, James D.; Cheng, Christine C.; Frye, Christopher C.; Bumol, Thomas F.; Moller, David E.

Year: 2013

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0058575

Paper Engineering the Expression and Characterization of Two Novel Laccase Isoenzymes from Coprinus comatus in Pichia pastoris by Fusing an Additional Ten Amino Acids Tag at N-Terminus

Chunjuan, Gu; Zheng, Fei; Long, Liangkun et al. · 2014 · PLoS ONE · DOI

Full title: Engineering the Expression and Characterization of Two Novel Laccase Isoenzymes from Coprinus comatus in Pichia pastoris by Fusing an Additional Ten Amino Acids Tag at N-Terminus

Authors: Chunjuan, Gu; Zheng, Fei; Long, Liangkun; Wang, Jing; Ding, Shaojun

Year: 2014

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0093912

Paper Fusing a Carbohydrate-Binding Module into the Aspergillus usamii β-Mannanase to Improve Its Thermostability and Cellulose-Binding Capacity by In Silico Design

Tang, Cunduo; Li, Jianfang; Wei, Xi-Huan et al. · 2013 · PLoS ONE · DOI

Full title: Fusing a Carbohydrate-Binding Module into the Aspergillus usamii β-Mannanase to Improve Its Thermostability and Cellulose-Binding Capacity by In Silico Design

Authors: Tang, Cunduo; Li, Jianfang; Wei, Xi-Huan; Min, Rou; Gao, Shujuan; Wang, Junqing; Yin, Xin; Wu, Minchen

Year: 2013

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0064766

Paper Expression and Purification of Integral Membrane Fatty Acid Desaturases

Chen, Haiqin; Gu, Zhennan; Zhang, Hao et al. · 2013 · PLoS ONE · DOI

Full title: Expression and Purification of Integral Membrane Fatty Acid Desaturases

Authors: Chen, Haiqin; Gu, Zhennan; Zhang, Hao; Wang, Mingxuan; Chen, Wei; Lowther, W. Todd; Chen, Yong Q.

Year: 2013

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0058139

Paper The pCri System: A Vector Collection for Recombinant Protein Expression and Purification

Goulas, Theodoros; Cuppari, Anna; García-Castellanos, Raquel et al. · 2014 · PLoS ONE · DOI

Full title: The pCri System: A Vector Collection for Recombinant Protein Expression and Purification

Authors: Goulas, Theodoros; Cuppari, Anna; García-Castellanos, Raquel; Snipas, Scott J.; Glockshuber, Rudi; Arolas, Joan L.; Gomis‐Rüth, F. Xavier

Year: 2014

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0112643

Paper Integration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism

Tomàs‐Gamisans, Màrius; Ferrer, Pau; Albiol, Joan · 2016 · PLoS ONE · DOI

Full title: Integration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism

Authors: Tomàs‐Gamisans, Màrius; Ferrer, Pau; Albiol, Joan

Year: 2016

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0148031

Paper Structural and Functional Roles of Glycosylation in Fungal Laccase from Lentinus sp.

Maestre‐Reyna, Manuel; Liu, Wei-Chun; Jeng, W.Y. et al. · 2015 · PLoS ONE · DOI

Full title: Structural and Functional Roles of Glycosylation in Fungal Laccase from Lentinus sp.

Authors: Maestre‐Reyna, Manuel; Liu, Wei-Chun; Jeng, W.Y.; Lee, Cheng‐Chung; Hsu, Chih-An; Wen, Tuan-Nan; Wang, Andrew H.‐J.; Shyur, Lie‐Fen

Year: 2015

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0120601

Paper Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E. coli

Roy, Moushree Pal; Mazumdar, Deepika; Dutta, Subhabrata et al. · 2016 · PLoS ONE · DOI

Full title: Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E. coli

Authors: Roy, Moushree Pal; Mazumdar, Deepika; Dutta, Subhabrata; Saha, Shyama Prasad; Ghosh, Shilpi

Year: 2016

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0145745

Paper High-Level Expression of Endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Application for the Deglycosylation of Glycoproteins

Wang, Fei; Wang, Xiaojuan; Yu, Xiaolan et al. · 2015 · PLoS ONE · DOI

Full title: High-Level Expression of Endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Application for the Deglycosylation of Glycoproteins

Authors: Wang, Fei; Wang, Xiaojuan; Yu, Xiaolan; Fu, Lin; Liu, Yunyun; Ma, Li‐Xin; Zhai, Chao

Year: 2015

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0120458

Paper Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris

Bryn Edwards-Jones; Rochelle Aw; G. Barton et al. · 2015 · PLoS ONE · DOI

Full title: Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris

Authors: Bryn Edwards-Jones; Rochelle Aw; G. Barton; Gregory D. Tredwell; J. Bundy; D. Leak

Year: 2015

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0119637

Paper Overexpression of a Novel Thermostable and Chloride-Tolerant Laccase from Thermus thermophilus SG0.5JP17-16 in Pichia pastoris and Its Application in Synthetic Dye Decolorization

Liu, Huiping; Cheng, Yu; Du, Bing et al. · 2015 · PLoS ONE · DOI

Full title: Overexpression of a Novel Thermostable and Chloride-Tolerant Laccase from Thermus thermophilus SG0.5JP17-16 in Pichia pastoris and Its Application in Synthetic Dye Decolorization

Authors: Liu, Huiping; Cheng, Yu; Du, Bing; Tong, Chaofan; Liang, Shuli; Han, Shuangyan; Zheng, Suiping; Lin, Ying

Year: 2015

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0119833

Paper A Highly Efficient Recombinant Laccase from the Yeast Yarrowia lipolytica and Its Application in the Hydrolysis of Biomass

Kalyani, Dayanand C.; Tiwari, Manish Kumar; Li, Jinglin et al. · 2015 · PLoS ONE · DOI

Full title: A Highly Efficient Recombinant Laccase from the Yeast Yarrowia lipolytica and Its Application in the Hydrolysis of Biomass

Authors: Kalyani, Dayanand C.; Tiwari, Manish Kumar; Li, Jinglin; Kim, Sun Chang; Kalia, Vipin Chandra; Kang, Yun Chan; Lee, Jung-Kul

Year: 2015

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0120156

Paper Biochemical studies of two lytic polysaccharide monooxygenases from the white-rot fungus Heterobasidion irregulare and their roles in lignocellulose degradation

Liu, Bing; Olson, Åke; Wu, Miao et al. · 2017 · PLoS ONE · DOI

Full title: Biochemical studies of two lytic polysaccharide monooxygenases from the white-rot fungus Heterobasidion irregulare and their roles in lignocellulose degradation

Authors: Liu, Bing; Olson, Åke; Wu, Miao; Broberg, Anders; Sandgren, Mats

Year: 2017

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0189479

Paper Improvement of bread making quality by supplementation with a recombinant xylanase produced by Pichia pastoris

Cunha, Carolina Cândida de Queiroz Brito; Gama, Aline Rodrigues; Cintra, Lorena Cardoso et al. · 2018 · PLoS ONE · DOI

Full title: Improvement of bread making quality by supplementation with a recombinant xylanase produced by Pichia pastoris

Authors: Cunha, Carolina Cândida de Queiroz Brito; Gama, Aline Rodrigues; Cintra, Lorena Cardoso; Bataus, Luiz Artur Mendes; Ulhôa, Cirano José

Year: 2018

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0192996

Paper Contribution of Disulfide Bridges to the Thermostability of a Type A Feruloyl Esterase from Aspergillus usamii

Yin, Xin; Hu, Die; Li, Jianfang et al. · 2015 · PLoS ONE · DOI

Full title: Contribution of Disulfide Bridges to the Thermostability of a Type A Feruloyl Esterase from Aspergillus usamii

Authors: Yin, Xin; Hu, Die; Li, Jianfang; He, Yao; Zhu, Tian-Di; Wu, Minchen

Year: 2015

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0126864

Paper Role of N-linked glycosylation in the enzymatic properties of a thermophilic GH 10 xylanase from Aspergillus fumigatus expressed in Pichia pastoris

Chang, Xiaoyu; Xu, Bo; Bai, Yingguo et al. · 2017 · PLoS ONE · DOI

Full title: Role of N-linked glycosylation in the enzymatic properties of a thermophilic GH 10 xylanase from Aspergillus fumigatus expressed in Pichia pastoris

Authors: Chang, Xiaoyu; Xu, Bo; Bai, Yingguo; Luo, Huiying; Ma, Rui; Shi, Pengjun; Yao, Bin

Year: 2017

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0171111

Paper Identification and Functional Characterization of Glycosylation of Recombinant Human Platelet-Derived Growth Factor-BB in Pichia pastoris

Dai, Mengmeng; Yu, Changming; Fang, Ting et al. · 2015 · PLoS ONE · DOI

Full title: Identification and Functional Characterization of Glycosylation of Recombinant Human Platelet-Derived Growth Factor-BB in Pichia pastoris

Authors: Dai, Mengmeng; Yu, Changming; Fang, Ting; Fu, Ling; Wang, Jing; Zhang, Jun; Ren, Jun; Xu, Junjie; Zhang, Xiaopeng; Chen, Wei

Year: 2015

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0145419

Paper Computational Analysis and Low-Scale Constitutive Expression of Laccases Synthetic Genes GlLCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris

Rivera-Hoyos, Claudia M.; Morales-Álvarez, Edwin D.; Cuevas, Sergio Alejandro Poveda et al. · 2015 · PLoS ONE · DOI

Full title: Computational Analysis and Low-Scale Constitutive Expression of Laccases Synthetic Genes GlLCC1 from Ganoderma lucidum and POXA 1B from Pleurotus ostreatus in Pichia pastoris

Authors: Rivera-Hoyos, Claudia M.; Morales-Álvarez, Edwin D.; Cuevas, Sergio Alejandro Poveda; Reyes-Guzmán, Edwin Alfredo; Poutou‐Piñales, Raúl A.; Reyes-Montaño, Edgar Antonio; Pedroza-Rodríguez, Aura M.; Rodríguez-Vázquez, Refugio; Cardozo-Bernal, Ángela M.

Year: 2015

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0116524

Paper Cofactor engineering improved CALB production in Pichia pastoris through heterologous expression of NADH oxidase and adenylate kinase

C. Jayachandran; Balakumaran Palanisamy Athiyaman; Meenakshisundaram Sankaranarayanan · 2017 · PLoS ONE · DOI

Full title: Cofactor engineering improved CALB production in Pichia pastoris through heterologous expression of NADH oxidase and adenylate kinase

Authors: C. Jayachandran; Balakumaran Palanisamy Athiyaman; Meenakshisundaram Sankaranarayanan

Year: 2017

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0181370

Paper Cloning, expression, and characterization of a recombinant xylanase from Bacillus sonorensis T6

Kiribayeva, Assel; Mukanov, Birzhan; Silayev, Dmitriy et al. · 2022 · PLoS ONE · DOI

Full title: Cloning, expression, and characterization of a recombinant xylanase from Bacillus sonorensis T6

Authors: Kiribayeva, Assel; Mukanov, Birzhan; Silayev, Dmitriy; Akishev, Zhiger; Ramankulov, Yerlan; Khassenov, Bekbolat

Year: 2022

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0265647

Paper Design, expression and functional characterization of a thermostable xylanase from Trichoderma reesei

He, Jun; Tang, Feng; Chen, Daiwen et al. · 2019 · PLoS ONE · DOI

Full title: Design, expression and functional characterization of a thermostable xylanase from Trichoderma reesei

Authors: He, Jun; Tang, Feng; Chen, Daiwen; Yu, Bing; Luo, Yuheng; Zheng, Ping; Mao, Xiangbing; Yu, Jie; Feng, Yu

Year: 2019

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0210548

Paper Production of an anti-Aβ antibody fragment in Pichia pastoris and in vitro and in vivo validation of its therapeutic effect

Montoliu‐Gaya, Laia; Esquerda-Canals, Gisela; Bronsoms, Sílvia et al. · 2017 · PLoS ONE · DOI

Full title: Production of an anti-Aβ antibody fragment in Pichia pastoris and in vitro and in vivo validation of its therapeutic effect

Authors: Montoliu‐Gaya, Laia; Esquerda-Canals, Gisela; Bronsoms, Sílvia; Villegas, Sandra

Year: 2017

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0181480

Paper Molecular expression of a recombinant thermostable bacterial amylase from Geobacillus stearothermophilus SR74 using methanol-free Meyerozyma guilliermondii strain SO yeast system

Nasir, Nurul Syahida Mohd; Leow, Chor T.; Oslan, Siti Nurbaya et al. · 2020 · BioResources · DOI

Full title: Molecular expression of a recombinant thermostable bacterial amylase from Geobacillus stearothermophilus SR74 using methanol-free Meyerozyma guilliermondii strain SO yeast system

Authors: Nasir, Nurul Syahida Mohd; Leow, Chor T.; Oslan, Siti Nurbaya; Salleh, Abu Bakar; Oslan, Siti Nurbaya

Year: 2020

Venue: BioResources

DOI: https://doi.org/10.15376/biores.15.2.3161-3172

Paper Recombinants proteins for industrial uses: utilization of Pichia pastoris expression system

Rabert, Claudia; Weinacker, Daniel; Pessoa, Adalberto et al. · 2013 · Brazilian Journal of Microbiology · DOI

Full title: Recombinants proteins for industrial uses: utilization of Pichia pastoris expression system

Authors: Rabert, Claudia; Weinacker, Daniel; Pessoa, Adalberto; Farías, Jorge G.

Year: 2013

Venue: Brazilian Journal of Microbiology

DOI: https://doi.org/10.1590/s1517-83822013005000041

Paper Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris

Teh, Ser Huy; Fong, Mun Yik; Mohamed, Zulqarnain · 2011 · Genetics and Molecular Biology · DOI

Full title: Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris

Authors: Teh, Ser Huy; Fong, Mun Yik; Mohamed, Zulqarnain

Year: 2011

Venue: Genetics and Molecular Biology

DOI: https://doi.org/10.1590/s1415-47572011005000022

Paper Applications of recombinant Pichia pastoris in the healthcare industry

Weinacker, Daniel; Rabert, Claudia; Zepeda, Andrea et al. · 2013 · Brazilian Journal of Microbiology · DOI

Full title: Applications of recombinant Pichia pastoris in the healthcare industry

Authors: Weinacker, Daniel; Rabert, Claudia; Zepeda, Andrea; Figueroa, Carolina A.; Pessoa, Adalberto; Farías, Jorge G.

Year: 2013

Venue: Brazilian Journal of Microbiology

DOI: https://doi.org/10.1590/s1517-83822013000400004

Paper ON-LINE MONITORING OF BIOMASS CONCENTRATION BASED ON A CAPACITANCE SENSOR: ASSESSING THE METHODOLOGY FOR DIFFERENT BACTERIA AND YEAST HIGH CELL DENSITY FED-BATCH CULTURES

Horta, Antônio Carlos Luperni; Silva, Adilson José da; Sargo, Cíntia Regina et al. · 2015 · Brazilian Journal of Chemical Engineering · DOI

Full title: ON-LINE MONITORING OF BIOMASS CONCENTRATION BASED ON A CAPACITANCE SENSOR: ASSESSING THE METHODOLOGY FOR DIFFERENT BACTERIA AND YEAST HIGH CELL DENSITY FED-BATCH CULTURES

Authors: Horta, Antônio Carlos Luperni; Silva, Adilson José da; Sargo, Cíntia Regina; Montano, Inti Doraci Cavalcanti; Galeano-Suarez, I. D.; Velez, Ana; Santos, Maurício Possedente dos; Gonçalves, Viviane Maimoni; Giordano, Roberto C.; Zangirolami, Teresa Cristina

Year: 2015

Venue: Brazilian Journal of Chemical Engineering

DOI: https://doi.org/10.1590/0104-6632.20150324s00003534

Paper Translational Modification and pH Optimization of Expression Media for High-Yield Recombinant Human-like Collagen (RHC) Production in Pichia pastoris GS115

Maruf Hasan; Zijie Li · 2025 · International Journal of Environment Agriculture and Biotechnology · DOI

Full title: Translational Modification and pH Optimization of Expression Media for High-Yield Recombinant Human-like Collagen (RHC) Production in Pichia pastoris GS115

Authors: Maruf Hasan; Zijie Li

Year: 2025

Venue: International Journal of Environment Agriculture and Biotechnology

DOI: https://doi.org/10.22161/ijeab.105.17

Paper Recombinant chymosin of Camelus dromedarius in Pichia pastoris expression system: Purification and Enzymatic Profile

E. Antonova; Natal'ya Firsova; Natal'ya Lengesova et al. · 2024 · Dairy Industry · DOI

Full title: Recombinant chymosin of Camelus dromedarius in Pichia pastoris expression system: Purification and Enzymatic Profile

Authors: E. Antonova; Natal'ya Firsova; Natal'ya Lengesova; Denis Viktorov; Atabeg B. Achilov; Pavel Torutanov

Year: 2024

Venue: Dairy Industry

DOI: https://doi.org/10.21603/1019-8946-2024-6-17

Paper Identification and Characterization of a Novel Hyperthermostable Bifunctional Cellobiohydrolase- Xylanase Enzyme for Synergistic Effect With Commercial Cellulase on Pretreated Wheat Straw Degradation

Han, Chao; Yang, Ruirui; Sun, Yanxu et al. · 2020 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Identification and Characterization of a Novel Hyperthermostable Bifunctional Cellobiohydrolase- Xylanase Enzyme for Synergistic Effect With Commercial Cellulase on Pretreated Wheat Straw Degradation

Authors: Han, Chao; Yang, Ruirui; Sun, Yanxu; Liu, Mengyu; Zhou, Lifan; Li, Duochuan

Year: 2020

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2020.00296

Paper Fluorescent Reporter Libraries as Useful Tools for Optimizing Microbial Cell Factories: A Review of the Current Methods and Applications

Delvigne, Frank; Pêcheux, Hélène; Tarayre, Cédric · 2015 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Fluorescent Reporter Libraries as Useful Tools for Optimizing Microbial Cell Factories: A Review of the Current Methods and Applications

Authors: Delvigne, Frank; Pêcheux, Hélène; Tarayre, Cédric

Year: 2015

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2015.00147

Paper Book Review: Recent Advances in Yeast Metabolic Engineering

Gohil, Nisarg; Panchasara, Happy; Patel, Shreya K. et al. · 2017 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Book Review: Recent Advances in Yeast Metabolic Engineering

Authors: Gohil, Nisarg; Panchasara, Happy; Patel, Shreya K.; Ramírez‐García, Robert; Singh, Vijai

Year: 2017

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2017.00071

Paper High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming

Zheng, Xueyun; Zhang, Yimin; Liu, Xiaoxiao et al. · 2020 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming

Authors: Zheng, Xueyun; Zhang, Yimin; Liu, Xiaoxiao; Cheng, Li; Lin, Ying; Liang, Shuli

Year: 2020

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2020.00850

Paper A Combinational Strategy for Effective Heterologous Production of Functional Human Lysozyme in Pichia pastoris.

He, Huahua; Wu, Shijie; Mei, Meng et al. · 2020 · Frontiers in bioengineering and biotechnology · DOI

Full title: A Combinational Strategy for Effective Heterologous Production of Functional Human Lysozyme in Pichia pastoris.

Authors: He, Huahua; Wu, Shijie; Mei, Meng; Ning, Jiali; Li, Chaoyin; Ma, Lixin; Zhang, Guimin; Yi, Li

Year: 2020

Venue: Frontiers in bioengineering and biotechnology

DOI: https://doi.org/10.3389/fbioe.2020.00118

Paper Industrial Use of Cell Wall Degrading Enzymes: The Fine Line Between Production Strategy and Economic Feasibility

Giovannoni, Moira; Gramegna, Giovanna; Benedetti, Manuel et al. · 2020 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Industrial Use of Cell Wall Degrading Enzymes: The Fine Line Between Production Strategy and Economic Feasibility

Authors: Giovannoni, Moira; Gramegna, Giovanna; Benedetti, Manuel; Mattei, Benedetta

Year: 2020

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2020.00356

Paper Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris

G. Naseri; Kevin Prause; H. H. Hamdo et al. · 2021 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris

Authors: G. Naseri; Kevin Prause; H. H. Hamdo; C. Arenz

Year: 2021

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2021.676900

Paper Saccharomyces cerevisiae cell surface display technology: Strategies for improvement and applications

Zhang, Chenmeng; Chen, Hongyu; Zhu, Yiping et al. · 2022 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Saccharomyces cerevisiae cell surface display technology: Strategies for improvement and applications

Authors: Zhang, Chenmeng; Chen, Hongyu; Zhu, Yiping; Zhang, Yu; Li, Xun; Wang, Fei

Year: 2022

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2022.1056804

Paper Thermostable Cellulases / Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective

Ajeje, Samaila Boyi; Hu, Yun; Song, Guojie et al. · 2021 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Thermostable Cellulases / Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective

Authors: Ajeje, Samaila Boyi; Hu, Yun; Song, Guojie; Peter, Sunday Bulus; Afful, Richmond Godwin; Sun, Fubao; Asadollahi, Mohammad Ali; Amiri, Hamid; Abdulkhani, Ali; Sun, Haiyan

Year: 2021

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2021.794304

Paper Multiplex Genome Engineering Methods for Yeast Cell Factory Development

Malcı, Koray; Walls, Laura E.; Rios‐Solis, Leonardo · 2020 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Multiplex Genome Engineering Methods for Yeast Cell Factory Development

Authors: Malcı, Koray; Walls, Laura E.; Rios‐Solis, Leonardo

Year: 2020

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2020.589468

Paper Identification and Expression of New Unspecific Peroxygenases – Recent Advances, Challenges and Opportunities

Kinner, Alina; Rosenthal, Katrin; Lütz, Stephan · 2021 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Identification and Expression of New Unspecific Peroxygenases – Recent Advances, Challenges and Opportunities

Authors: Kinner, Alina; Rosenthal, Katrin; Lütz, Stephan

Year: 2021

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2021.705630

Paper Improved Production of Xylanase in Pichia pastoris and Its Application in Xylose Production From Xylan

Ting Miao; A. Basit; Junquan Liu et al. · 2021 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Improved Production of Xylanase in Pichia pastoris and Its Application in Xylose Production From Xylan

Authors: Ting Miao; A. Basit; Junquan Liu; Fengzhen Zheng; Kashif Rahim; H. Lou; Wei Jiang

Year: 2021

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2021.690702

Paper Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production

Arnau Gasset; Xavier Garcia-Ortega; Javier Garrigós-Martínez et al. · 2022 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production

Authors: Arnau Gasset; Xavier Garcia-Ortega; Javier Garrigós-Martínez; F. Valero; José Luis Montesinos-Seguí

Year: 2022

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2022.818434

Paper Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications

Teymennet‐Ramírez, Karla Verónica; Martínez‐Morales, Fernando; Trejo‐Hernández, María R. · 2022 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications

Authors: Teymennet‐Ramírez, Karla Verónica; Martínez‐Morales, Fernando; Trejo‐Hernández, María R.

Year: 2022

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2021.794742

Paper Developing methylotrophic microbial platforms for a methanol-based bioindustry

Singh, Hawaibam Birla; Kang, Min‐Kyoung; Kwon, Moonhyuk et al. · 2022 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Developing methylotrophic microbial platforms for a methanol-based bioindustry

Authors: Singh, Hawaibam Birla; Kang, Min‐Kyoung; Kwon, Moonhyuk; Kim, Seon-Won

Year: 2022

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2022.1050740

Paper Murine Factor H Co-Produced in Yeast With Protein Disulfide Isomerase Ameliorated C3 Dysregulation in Factor H-Deficient Mice

Kerr, Heather; Herbert, Andrew P.; Makou, Elisavet et al. · 2021 · Frontiers in Immunology · DOI

Full title: Murine Factor H Co-Produced in Yeast With Protein Disulfide Isomerase Ameliorated C3 Dysregulation in Factor H-Deficient Mice

Authors: Kerr, Heather; Herbert, Andrew P.; Makou, Elisavet; Abramczyk, Dariusz; Malik, Talat H.; Lomax-Browne, Hannah J.; Yang, Yi; Pappworth, Isabel Y.; Denton, Harriet; Richards, Anna; Marchbank, Kevin J.; Pickering, Matthew C.; Barlow, Paul N.

Year: 2021

Venue: Frontiers in Immunology

DOI: https://doi.org/10.3389/fimmu.2021.681098

Paper Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free PDH promoter

Bernat-Camps, Núria; Ebner, Katharina; Schusterbauer, Veronika et al. · 2023 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free PDH promoter

Authors: Bernat-Camps, Núria; Ebner, Katharina; Schusterbauer, Veronika; Fischer, Jasmin Elgin; Nieto‐Taype, Miguel Angel; Valero, Francisco; Glieder, Anton; Garcia‐Ortega, Xavier

Year: 2023

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2023.1130583

Paper Bioprospecting Kluyveromyces marxianus as a Robust Host for Industrial Biotechnology

Bilal, Muhammad; Ji, Liyun; Xu, Yirong et al. · 2022 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Bioprospecting Kluyveromyces marxianus as a Robust Host for Industrial Biotechnology

Authors: Bilal, Muhammad; Ji, Liyun; Xu, Yirong; Xu, Shuo; Lin, Yuping; Iqbal, Hafiz M.N.; Cheng, Hairong

Year: 2022

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2022.851768

Paper Synthetic Biology and Metabolic Engineering Approaches and Its Impact on Non-Conventional Yeast and Biofuel Production

Madhavan, Aravind; Jose, Anju Alphonsa; Binod, Parameswaran et al. · 2017 · Frontiers in Energy Research · DOI

Full title: Synthetic Biology and Metabolic Engineering Approaches and Its Impact on Non-Conventional Yeast and Biofuel Production

Authors: Madhavan, Aravind; Jose, Anju Alphonsa; Binod, Parameswaran; Sindhu, Raveendran; Sukumaran, Rajeev K.; Pandey, Ashok; Castro, Eulógio

Year: 2017

Venue: Frontiers in Energy Research

DOI: https://doi.org/10.3389/fenrg.2017.00008

Paper Yeasts as Biopharmaceutical Production Platforms

Kulagina, Natalja; Besseau, Sébastien; Godon, Charlotte et al. · 2021 · Frontiers in Fungal Biology · DOI

Full title: Yeasts as Biopharmaceutical Production Platforms

Authors: Kulagina, Natalja; Besseau, Sébastien; Godon, Charlotte; Goldman, Gustavo H.; Papon, Nicolas; Courdavault, Vincent

Year: 2021

Venue: Frontiers in Fungal Biology

DOI: https://doi.org/10.3389/ffunb.2021.733492

Paper Expression of codon optimized genes in microbial systems: current industrial applications and perspectives

Elena, Claudia; Ravasi, Pablo; Castelli, María Eugenia et al. · 2014 · Frontiers in Microbiology · DOI

Full title: Expression of codon optimized genes in microbial systems: current industrial applications and perspectives

Authors: Elena, Claudia; Ravasi, Pablo; Castelli, María Eugenia; Peirú, Salvador; Menzella, Hugo G.

Year: 2014

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2014.00021

Paper Recombinant protein expression in microbial systems

Rosano, Germán L.; Ceccarelli, Eduardo A. · 2014 · Frontiers in Microbiology · DOI

Full title: Recombinant protein expression in microbial systems

Authors: Rosano, Germán L.; Ceccarelli, Eduardo A.

Year: 2014

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2014.00341

Paper Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma reesei

Wang, Min; Lü, Xuefeng · 2016 · Frontiers in Microbiology · DOI

Full title: Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma reesei

Authors: Wang, Min; Lü, Xuefeng

Year: 2016

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2016.00620

Paper Playing catch-up with Escherichia coli: using yeast to increase success rates in recombinant protein production experiments

Bill, Roslyn M. · 2014 · Frontiers in Microbiology · DOI

Full title: Playing catch-up with Escherichia coli: using yeast to increase success rates in recombinant protein production experiments

Authors: Bill, Roslyn M.

Year: 2014

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2014.00085

Paper Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris

Haon, Mireille; Grisel, Sacha; Navarro, David et al. · 2015 · Frontiers in Microbiology · DOI

Full title: Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris

Authors: Haon, Mireille; Grisel, Sacha; Navarro, David; Gruet, Antoine; Berrin, Jean‐Guy; Bignon, Christophe

Year: 2015

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2015.01002

Paper A comparative summary of expression systems for the recombinant production of galactose oxidase

Spadiut, Oliver; Olsson, Lisbeth; Brumer, Harry · 2010 · Microbial Cell Factories · DOI

Full title: A comparative summary of expression systems for the recombinant production of galactose oxidase

Authors: Spadiut, Oliver; Olsson, Lisbeth; Brumer, Harry

Year: 2010

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-9-68

Paper Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Jacobs, Pieter P.; İnan, Mehmet; Festjens, Nele et al. · 2010 · Microbial Cell Factories · DOI

Full title: Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Authors: Jacobs, Pieter P.; İnan, Mehmet; Festjens, Nele; Haustraete, Jurgen; Hecke, Annelies Van; Meagher, Michael M.; Contreras, Roland; Callewaert, Nico

Year: 2010

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-9-93

Paper A multi-level study of recombinant Pichia pastoris in different oxygen conditions

Baumann, Kristin; Carnicer, Marc; Dragosits, Martin et al. · 2010 · BMC Systems Biology · DOI

Full title: A multi-level study of recombinant Pichia pastoris in different oxygen conditions

Authors: Baumann, Kristin; Carnicer, Marc; Dragosits, Martin; Graf, Alexandra B.; Stadlmann, Johannes; Jouhten, Paula; Maaheimo, Hannu; Gasser, Brigitte; Albiol, Joan; Mattanovich, Diethard; Ferrer, Pau

Year: 2010

Venue: BMC Systems Biology

DOI: https://doi.org/10.1186/1752-0509-4-141

Paper High yield recombinant thermostable α-amylase production using an improved Bacillus licheniformis system

Niu, Dandan; Zhirui, Zuo; Shi, Guiyang et al. · 2009 · Microbial Cell Factories · DOI

Full title: High yield recombinant thermostable α-amylase production using an improved Bacillus licheniformis system

Authors: Niu, Dandan; Zhirui, Zuo; Shi, Guiyang; Wang, Zhengxiang

Year: 2009

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-8-58

Paper Cloning of the Zygosaccharomyces bailii GAS 1 homologue and effect of cell wall engineering on protein secretory phenotype

Passolunghi, Simone; Riboldi, Luca; Dato, Laura et al. · 2010 · Microbial Cell Factories · DOI

Full title: Cloning of the Zygosaccharomyces bailii GAS 1 homologue and effect of cell wall engineering on protein secretory phenotype

Authors: Passolunghi, Simone; Riboldi, Luca; Dato, Laura; Porro, Danilo; Branduardi, Paola

Year: 2010

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-9-7

Paper Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus

Rocha, Saul Nitsche; Abrahão-Neto, José; Cerdán, M. Esperanza et al. · 2010 · Microbial Cell Factories · DOI

Full title: Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus

Authors: Rocha, Saul Nitsche; Abrahão-Neto, José; Cerdán, M. Esperanza; González-Siso, María-Isabel; Gombert, Andreas

Year: 2010

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/1475-2859-9-4

Paper Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel

Huang, Jinjin; Xia, Ji; Yang, Zhen et al. · 2014 · Biotechnology for Biofuels · DOI

Full title: Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel

Authors: Huang, Jinjin; Xia, Ji; Yang, Zhen; Guan, Feifei; Cui, Di; Guan, Guohua; Jiang, Wei; Li, Ying

Year: 2014

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/1754-6834-7-111

Paper Computational codon optimization of synthetic gene for protein expression

Chung, Bevan Kai-Sheng; Lee, Dong‐Yup · 2012 · BMC Systems Biology · DOI

Full title: Computational codon optimization of synthetic gene for protein expression

Authors: Chung, Bevan Kai-Sheng; Lee, Dong‐Yup

Year: 2012

Venue: BMC Systems Biology

DOI: https://doi.org/10.1186/1752-0509-6-134

Paper High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production

Yu, Xiao‐Wei; Sha, Chong; Guo, Yong-Liang et al. · 2013 · Biotechnology for Biofuels · DOI

Full title: High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production

Authors: Yu, Xiao‐Wei; Sha, Chong; Guo, Yong-Liang; Xiao, Rong; Xu, Yan

Year: 2013

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/1754-6834-6-29

Paper Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice

Athmaram, TN; Saraswat, Shweta; Santhosh, SR et al. · 2011 · Virology Journal · DOI

Full title: Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice

Authors: Athmaram, TN; Saraswat, Shweta; Santhosh, SR; Singh, Ajay; Suryanarayana, VVS; Priya, Raj; Gopalan, Natarajan; Parida, Manmohan; Rao, PV Lakshmana; Vijayaraghavan, R.

Year: 2011

Venue: Virology Journal

DOI: https://doi.org/10.1186/1743-422x-8-524

Paper Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary 13C flux analysis

Jordà, Joel; Suarez-Mendez, Camilo; Carnicer, Marc et al. · 2013 · BMC Systems Biology · DOI

Full title: Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary 13C flux analysis

Authors: Jordà, Joel; Suarez-Mendez, Camilo; Carnicer, Marc; Pierick, Angela ten; Heijnen, Joseph J.; Gulik, Walter van; Ferrer, Pau; Albiol, Joan; Wahl, A.

Year: 2013

Venue: BMC Systems Biology

DOI: https://doi.org/10.1186/1752-0509-7-17

Paper Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica

Bulani, Siyavuya Ishmael; Moleleki, Lucy; Albertyn, Jacobus et al. · 2012 · AMB Express · DOI

Full title: Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica

Authors: Bulani, Siyavuya Ishmael; Moleleki, Lucy; Albertyn, Jacobus; Moleleki, Ntsane

Year: 2012

Venue: AMB Express

DOI: https://doi.org/10.1186/2191-0855-2-27

Paper High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

Ilmén, Marja; Haan, Riaan den; Brevnova, Elena E. et al. · 2011 · Biotechnology for Biofuels · DOI

Full title: High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

Authors: Ilmén, Marja; Haan, Riaan den; Brevnova, Elena E.; McBride, John E.; Wiswall, Erin; Froehlich, Allan C.; Koivula, Anu; Voutilainen, Sanni; Siika‐aho, Matti; Grange, Daniël C. la; Thorngren, Naomi; Ahlgren, Simon; Mellon, Mark T.; Deleault, Kristen M.; Rajgarhia, Vineet; Zyl, Willem H. van; Penttilä, Merja

Year: 2011

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/1754-6834-4-30

Paper Implications of cellobiohydrolase glycosylation for use in biomass conversion

Jeoh, Tina; Michener, William E.; Himmel, Michael E. et al. · 2008 · Biotechnology for Biofuels · DOI

Full title: Implications of cellobiohydrolase glycosylation for use in biomass conversion

Authors: Jeoh, Tina; Michener, William E.; Himmel, Michael E.; Decker, Stephen R.; Adney, William S.

Year: 2008

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/1754-6834-1-10

Paper Construction of a novel Pichia pastoris strain for production of xanthophylls

Araya-Garay, José Miguel; Ageitos, José Manuel; Vallejo, Juán A. et al. · 2012 · AMB Express · DOI

Full title: Construction of a novel Pichia pastoris strain for production of xanthophylls

Authors: Araya-Garay, José Miguel; Ageitos, José Manuel; Vallejo, Juán A.; Veiga‐Crespo, Patricia; Sánchez-Pérez, Ángeles; Villa, Tomás G.

Year: 2012

Venue: AMB Express

DOI: https://doi.org/10.1186/2191-0855-2-24

Paper Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring

Abdulrachman, Dede; Thongkred, Paweena; Kocharin, Kanokarn et al. · 2017 · BMC Biotechnology · DOI

Full title: Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring

Authors: Abdulrachman, Dede; Thongkred, Paweena; Kocharin, Kanokarn; Nakpathom, Monthon; Somboon, Buppha; Narumol, Nootsara; Champreda, Verawat; Eurwilaichitr, Lily; Suwanto, Antonius; Nimchua, Thidarat; Chantasingh, Duriya

Year: 2017

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/s12896-017-0334-9

Paper Comparative genomics and transcriptomics of Pichia pastoris

M. Priest; Jonathan Borowsky; Sarah K. Young et al. · 2016 · BMC Genomics · DOI

Full title: Comparative genomics and transcriptomics of Pichia pastoris

Authors: M. Priest; Jonathan Borowsky; Sarah K. Young; K. Love; Charles A. Whittaker; J. C. Love; Jie Wu; Duanduan Ma; Kartik Shah; M. C. Bartlett; Rachel Leeson

Year: 2016

Venue: BMC Genomics

DOI: https://doi.org/10.1186/s12864-016-2876-y

Paper Genome-scale metabolic reconstructions of Pichia stipitis and Pichia pastoris and in silico evaluation of their potentials

Caspeta, Luis; Shoaie, Saeed; Ågren, Rasmus et al. · 2012 · BMC Systems Biology · DOI

Full title: Genome-scale metabolic reconstructions of Pichia stipitis and Pichia pastoris and in silico evaluation of their potentials

Authors: Caspeta, Luis; Shoaie, Saeed; Ågren, Rasmus; Nookaew, Intawat; Nielsen, Jens

Year: 2012

Venue: BMC Systems Biology

DOI: https://doi.org/10.1186/1752-0509-6-24

Paper Integrated lipase production and in situ biodiesel synthesis in a recombinant Pichia pastoris yeast: an efficient dual biocatalytic system composed of cell free enzymes and whole cell catalysts

Yan, Jinyong; Li, Zhaofeng; Du, Lei et al. · 2014 · Biotechnology for Biofuels · DOI

Full title: Integrated lipase production and in situ biodiesel synthesis in a recombinant Pichia pastoris yeast: an efficient dual biocatalytic system composed of cell free enzymes and whole cell catalysts

Authors: Yan, Jinyong; Li, Zhaofeng; Du, Lei; Li, Shengying

Year: 2014

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/1754-6834-7-55

Paper A new acidophilic thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris

Liao, Hanpeng; Li, Shuixian; Zheng, Haiping et al. · 2014 · BMC Biotechnology · DOI

Full title: A new acidophilic thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris

Authors: Liao, Hanpeng; Li, Shuixian; Zheng, Haiping; Wei, Zhong; Liu, Dongyang; Raza, Waseem; Shen, Qirong; Xu, Yangchun

Year: 2014

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/s12896-014-0090-z

Paper Cloning and expression of Aspergillus flavus urate oxidase in Pichia pastoris

Fazel, Ramin; Zarei, Najmeh; Ghaemi, Nasser et al. · 2014 · SpringerPlus · DOI

Full title: Cloning and expression of Aspergillus flavus urate oxidase in Pichia pastoris

Authors: Fazel, Ramin; Zarei, Najmeh; Ghaemi, Nasser; Namvaran, Mohammad Mehdi; Enayati, Somayeh; Mirabzadeh, Esmat; Azizi, Mohammad; Khalaj, Vahid

Year: 2014

Venue: SpringerPlus

DOI: https://doi.org/10.1186/2193-1801-3-395

Paper Virus-like particles derived from Pichia pastoris-expressed dengue virus type 1 glycoprotein elicit homotypic virus-neutralizing envelope domain III-directed antibodies

Poddar, Ankur; Ramasamy, Viswanathan; Shukla, Rahul et al. · 2016 · BMC Biotechnology · DOI

Full title: Virus-like particles derived from Pichia pastoris-expressed dengue virus type 1 glycoprotein elicit homotypic virus-neutralizing envelope domain III-directed antibodies

Authors: Poddar, Ankur; Ramasamy, Viswanathan; Shukla, Rahul; Rajpoot, Ravi Kant; Arora, Upasana; Jain, Swatantra Kumar; Swaminathan, S.; Khanna, Navin

Year: 2016

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/s12896-016-0280-y

Paper Dynamic genome-scale metabolic modeling of the yeast Pichia pastoris

F. Saitua; Paulina Torres; J. Pérez-Correa et al. · 2017 · BMC Systems Biology · DOI

Full title: Dynamic genome-scale metabolic modeling of the yeast Pichia pastoris

Authors: F. Saitua; Paulina Torres; J. Pérez-Correa; E. Agosin

Year: 2017

Venue: BMC Systems Biology

DOI: https://doi.org/10.1186/s12918-017-0408-2

Paper Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation

Shang, Tingting; Si, Dayong; Zhang, Dongyan et al. · 2017 · BMC Biotechnology · DOI

Full title: Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation

Authors: Shang, Tingting; Si, Dayong; Zhang, Dongyan; Liu, Xuhui; Zhao, Longmei; Hu, Cong; Fu, Yu; Zhang, Rijun

Year: 2017

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/s12896-017-0361-6

Paper An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)

Che, Zhiqun; Cao, Xiaoyan; Chen, Guiguang et al. · 2020 · BMC Biotechnology · DOI

Full title: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)

Authors: Che, Zhiqun; Cao, Xiaoyan; Chen, Guiguang; Liang, Zhiqun

Year: 2020

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/s12896-020-00654-7

Paper Expression and characteristics of manganese peroxidase from Ganoderma lucidum in Pichia pastoris and its application in the degradation of four dyes and phenol

Xu, Hui; Guo, Mengyuan; Gao, Yanhua et al. · 2017 · BMC Biotechnology · DOI

Full title: Expression and characteristics of manganese peroxidase from Ganoderma lucidum in Pichia pastoris and its application in the degradation of four dyes and phenol

Authors: Xu, Hui; Guo, Mengyuan; Gao, Yanhua; Bai, Xiaohui; Zhou, Xuanwei

Year: 2017

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/s12896-017-0338-5

Paper Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity

Yan, Guangbo; Min, Shu; Shen, Wei et al. · 2021 · BMC Biotechnology · DOI

Full title: Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity

Authors: Yan, Guangbo; Min, Shu; Shen, Wei; Ma, Lixin; Zhai, Chao; Wang, Yaping; Huang, Zunxi

Year: 2021

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/s12896-021-00708-4

Paper Casamino acids facilitate the secretion of recombinant dengue virus serotype-3 envelope domain III in Pichia pastoris

Kaushik, Neha; Rohila, Deepak; Arora, Upasana et al. · 2016 · BMC Biotechnology · DOI

Full title: Casamino acids facilitate the secretion of recombinant dengue virus serotype-3 envelope domain III in Pichia pastoris

Authors: Kaushik, Neha; Rohila, Deepak; Arora, Upasana; Raut, Rajendra; Lamminmäki, Urpo; Khanna, Navin; Batra, Gaurav

Year: 2016

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/s12896-016-0243-3

Paper Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris

Cheng Li; Ying-Wu Lin; Xueyun Zheng et al. · 2015 · BMC Biotechnology · DOI

Full title: Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris

Authors: Cheng Li; Ying-Wu Lin; Xueyun Zheng; Nuo Pang; Xihao Liao; Xiaoxiao Liu; Yuanyuan Huang; Shuli Liang

Year: 2015

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/s12896-015-0204-2

Paper Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Song, Xiaoping; Shao, Changsheng; Guo, Yugang et al. · 2019 · BMC Biotechnology · DOI

Full title: Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Authors: Song, Xiaoping; Shao, Changsheng; Guo, Yugang; Wang, Yajie; Cai, Jingjing

Year: 2019

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/s12896-019-0542-6

Paper Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks

Bawa, Zharain; Routledge, Sarah J; Jamshad, Mohammed et al. · 2014 · Microbial Cell Factories · DOI

Full title: Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks

Authors: Bawa, Zharain; Routledge, Sarah J; Jamshad, Mohammed; Clare, Michelle; Sarkar, Debasmita; Dickerson, Ian M.; Ganzlin, Markus; Poyner, David R.; Bill, Roslyn M.

Year: 2014

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-014-0127-y

Paper Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

Chang, Ching-Hsiang; Hsiung, Hao-An; Hong, Kai‐Lin et al. · 2018 · BMC Biotechnology · DOI

Full title: Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

Authors: Chang, Ching-Hsiang; Hsiung, Hao-An; Hong, Kai‐Lin; Huang, Ching‐Tsan

Year: 2018

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/s12896-018-0492-4

Paper Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganoderma Species

Qu, Zhengwei; Zhou, Siya; Guan, Shixin et al. · 2018 · BMC Biotechnology · DOI

Full title: Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganoderma Species

Authors: Qu, Zhengwei; Zhou, Siya; Guan, Shixin; Gao, Rui; Duan, Zuo-wen; Zhang, Xin; Sun, Wei-Yan; Fan, Wenli; Chen, Shuisen; Chen, Lijing; Lin, Jingwei; Ruan, Yanye

Year: 2018

Venue: BMC Biotechnology

DOI: https://doi.org/10.1186/s12896-018-0488-0

Paper Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

Rüth, Claudia; Buchetics, Markus; Vidimce, Viktorija et al. · 2014 · Microbial Cell Factories · DOI

Full title: Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

Authors: Rüth, Claudia; Buchetics, Markus; Vidimce, Viktorija; Kotz, Daniela; Naschberger, Stefan; Mattanovich, Diethard; Pichler, Harald; Gasser, Brigitte

Year: 2014

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-014-0120-5

Paper GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris

R. Prielhofer; Juan J. Barrero; Stefanie Steuer et al. · 2017 · BMC Systems Biology · DOI

Full title: GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris

Authors: R. Prielhofer; Juan J. Barrero; Stefanie Steuer; T. Gassler; Richard J. Zahrl; Kristin Baumann; M. Sauer; D. Mattanovich; Brigitte Gasser; Hans Marx

Year: 2017

Venue: BMC Systems Biology

DOI: https://doi.org/10.1186/s12918-017-0492-3

Paper Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation and by suppression of vacuolar targeting

Fitzgerald, Ivy; Glick, Benjamin S. · 2014 · Microbial Cell Factories · DOI

Full title: Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation and by suppression of vacuolar targeting

Authors: Fitzgerald, Ivy; Glick, Benjamin S.

Year: 2014

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-014-0125-0

Paper Cell factories for insulin production

Baeshen, Nabih A.; Baeshen, Mohammed N.; Sheikh, Abdullah et al. · 2014 · Microbial Cell Factories · DOI

Full title: Cell factories for insulin production

Authors: Baeshen, Nabih A.; Baeshen, Mohammed N.; Sheikh, Abdullah; Bora, Roop Singh; Ahmed, Mohamed Morsi M.; Ramadan, Hassan A. I.; Saini, Kulvinder Singh; Redwan, Elrashdy M.

Year: 2014

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-014-0141-0

Paper Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus

Yarimizu, Tohru; Nakamura, Mikiko; Hoshida, Hisashi et al. · 2015 · Microbial Cell Factories · DOI

Full title: Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus

Authors: Yarimizu, Tohru; Nakamura, Mikiko; Hoshida, Hisashi; Akada, Rinji

Year: 2015

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-015-0203-y

Paper High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress and decreases production of human interleukin-10

Zhong, Yongjun; Yang, Lu; Guo, Yugang et al. · 2014 · Microbial Cell Factories · DOI

Full title: High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress and decreases production of human interleukin-10

Authors: Zhong, Yongjun; Yang, Lu; Guo, Yugang; Fang, Fang; Wang, Dong; Li, Rui; Jiang, Ming; Kang, Wenyao; Ma, Jiajia; Sun, Jie; Xiao, Weihua

Year: 2014

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-014-0163-7

Paper Role of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichia pastoris

Yang, Min; Yu, Xiao‐Wei; Zheng, Haiyan et al. · 2015 · Microbial Cell Factories · DOI

Full title: Role of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichia pastoris

Authors: Yang, Min; Yu, Xiao‐Wei; Zheng, Haiyan; Sha, Chong; Zhao, Caifeng; Qian, Meiqian; Xu, Yan

Year: 2015

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-015-0225-5

Paper Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems

Cristina, Pietro Della; Castagna, Monica; Lombardi, Alessio et al. · 2015 · Microbial Cell Factories · DOI

Full title: Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems

Authors: Cristina, Pietro Della; Castagna, Monica; Lombardi, Alessio; Barison, Erika; Tagliabue, Giovanni; Ceriotti, Aldo; Koutris, Ilias; Leandro, Luana Di; Giansanti, Francesco; Vago, Riccardo; Ippoliti, Rodolfo; Flavell, Sopsamorn U.; Flavell, David J.; Colombatti, Marco; Fabbrini, Maria Serena

Year: 2015

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-015-0202-z

Paper Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

Gmeiner, Christoph; Saadati, Amirhossein; Maresch, Daniel et al. · 2015 · Microbial Cell Factories · DOI

Full title: Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

Authors: Gmeiner, Christoph; Saadati, Amirhossein; Maresch, Daniel; Krasteva, Stanimira; Frank, Manuela; Altmann, Friedrich; Herwig, Christoph; Spadiut, Oliver

Year: 2015

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-014-0183-3

Paper Construction of efficient xylose utilizing Pichia pastoris for industrial enzyme production

Pengfei Li; Hongbing Sun; Zao Chen et al. · 2015 · Microbial Cell Factories · DOI

Full title: Construction of efficient xylose utilizing Pichia pastoris for industrial enzyme production

Authors: Pengfei Li; Hongbing Sun; Zao Chen; Yin Li; T. Zhu

Year: 2015

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-015-0206-8

Paper Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris

Vogl, Thomas; Ahmad, Mudassar; Krainer, Florian et al. · 2015 · Microbial Cell Factories · DOI

Full title: Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris

Authors: Vogl, Thomas; Ahmad, Mudassar; Krainer, Florian; Schwab, Helmut; Glieder, Anton

Year: 2015

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-015-0293-6

Paper Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris

Florian W. Krainer; Simona Capone; Martin Jäger et al. · 2015 · Microbial Cell Factories · DOI

Full title: Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris

Authors: Florian W. Krainer; Simona Capone; Martin Jäger; T. Vogl; M. Gerstmann; A. Glieder; C. Herwig; O. Spadiut

Year: 2015

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-014-0187-z

Paper Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs

Marco, Ario de · 2015 · Microbial Cell Factories · DOI

Full title: Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs

Authors: Marco, Ario de

Year: 2015

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-015-0320-7

Paper ATP regulation in bioproduction

Hara, Kiyotaka Y.; Kondo, Akihiko · 2015 · Microbial Cell Factories · DOI

Full title: ATP regulation in bioproduction

Authors: Hara, Kiyotaka Y.; Kondo, Akihiko

Year: 2015

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-015-0390-6

Paper Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli

Chen, Ana; Sun, Yang; Zhang, Wei et al. · 2016 · Microbial Cell Factories · DOI

Full title: Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli

Authors: Chen, Ana; Sun, Yang; Zhang, Wei; Peng, Feng; Zhan, Chunjun; Liu, Meng; Yang, Yankun; Bai, Zhonghu

Year: 2016

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-015-0403-5

Paper Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter

Capone, Simona; Horvat, Jernej; Herwig, Christoph et al. · 2015 · Microbial Cell Factories · DOI

Full title: Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter

Authors: Capone, Simona; Horvat, Jernej; Herwig, Christoph; Spadiut, Oliver

Year: 2015

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-015-0292-7

Paper Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology

Brigitte Gasser; Matthias G. Steiger; D. Mattanovich · 2015 · Microbial Cell Factories · DOI

Full title: Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology

Authors: Brigitte Gasser; Matthias G. Steiger; D. Mattanovich

Year: 2015

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-015-0387-1

Paper The sweet branch of metabolic engineering: cherry-picking the low-hanging sugary fruits

Chen, Rachel · 2015 · Microbial Cell Factories · DOI

Full title: The sweet branch of metabolic engineering: cherry-picking the low-hanging sugary fruits

Authors: Chen, Rachel

Year: 2015

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-015-0389-z

Paper Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris

Aw, Rochelle; Polizzi, Karen M. · 2016 · Microbial Cell Factories · DOI

Full title: Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris

Authors: Aw, Rochelle; Polizzi, Karen M.

Year: 2016

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-016-0432-8

Paper Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization

Schwarzhans, Jan-Philipp; Wibberg, Daniel; Winkler, Anika et al. · 2016 · Microbial Cell Factories · DOI

Full title: Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization

Authors: Schwarzhans, Jan-Philipp; Wibberg, Daniel; Winkler, Anika; Luttermann, Tobias; Kalinowski, Jörn; Friehs, Karl

Year: 2016

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-016-0486-7

Paper Enhancing antibody folding and secretion by tailoring the Saccharomyces cerevisiae endoplasmic reticulum

Ruijter, Jorg C. de; Koskela, Essi V.; Frey, Alexander D. · 2016 · Microbial Cell Factories · DOI

Full title: Enhancing antibody folding and secretion by tailoring the Saccharomyces cerevisiae endoplasmic reticulum

Authors: Ruijter, Jorg C. de; Koskela, Essi V.; Frey, Alexander D.

Year: 2016

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-016-0488-5

Paper A high-throughput expression screening platform to optimize the production of antimicrobial peptides

Schreiber, Christine; Müller, Hagen; Birrenbach, Oliver et al. · 2017 · Microbial Cell Factories · DOI

Full title: A high-throughput expression screening platform to optimize the production of antimicrobial peptides

Authors: Schreiber, Christine; Müller, Hagen; Birrenbach, Oliver; Klein, Moritz; Heerd, Doreen; Weidner, Tobias; Salzig, Denise; Czermak, Peter

Year: 2017

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-017-0637-5

Paper Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica

Sassi, Hosni; Delvigne, Frank; Kar, Tambi et al. · 2016 · Microbial Cell Factories · DOI

Full title: Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica

Authors: Sassi, Hosni; Delvigne, Frank; Kar, Tambi; Nicaud, Jean‐Marc; Coq, Anne-Marie Crutz-Le; Steels, Sébastien; Fickers, Patrick

Year: 2016

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-016-0558-8

Paper A novel methanol-free Pichia pastoris system for recombinant protein expression

Shen, Wei; Xue, Ying; Liu, Yiqi et al. · 2016 · Microbial Cell Factories · DOI

Full title: A novel methanol-free Pichia pastoris system for recombinant protein expression

Authors: Shen, Wei; Xue, Ying; Liu, Yiqi; Kong, Chuixing; Wang, Xiaolong; Huang, Mengmeng; Cai, Menghao; Zhou, Xiangshan; Zhang, Yuanxing; Zhou, Mian

Year: 2016

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-016-0578-4

Paper Functional inclusion bodies produced in the yeast Pichia pastoris

Rueda, Fabián; Gasser, Brigitte; Sánchez‐Chardi, Alejandro et al. · 2016 · Microbial Cell Factories · DOI

Full title: Functional inclusion bodies produced in the yeast Pichia pastoris

Authors: Rueda, Fabián; Gasser, Brigitte; Sánchez‐Chardi, Alejandro; Roldán, Mónica; Villegas, Sandra; Puxbaum, Verena; Ferrer‐Miralles, Neus; Unzueta, Ugutz; Vázquez, Esther; García‐Fruitós, Elena; Mattanovich, Diethard; Villaverde, Antonio

Year: 2016

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-016-0565-9

Paper Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae

Xu, Sha; Geyuan, Zhang; Zhang, Huijie et al. · 2016 · Microbial Cell Factories · DOI

Full title: Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae

Authors: Xu, Sha; Geyuan, Zhang; Zhang, Huijie; Kitajima, Toshihiko; Nakanishi, Hideki; Gao, Xiao‐Dong

Year: 2016

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-016-0575-7

Paper Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source

Schotte, Peter; Dewerte, Isabelle; Groeve, Manu De et al. · 2016 · Microbial Cell Factories · DOI

Full title: Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source

Authors: Schotte, Peter; Dewerte, Isabelle; Groeve, Manu De; Keyser, Saskia De; Brabandere, Veronique De; Stanssens, Patrick

Year: 2016

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-016-0499-2

Paper A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis

Cai, Dongbo; Wang, Hao; He, Penghui et al. · 2017 · Microbial Cell Factories · DOI

Full title: A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis

Authors: Cai, Dongbo; Wang, Hao; He, Penghui; Zhu, Chengjun; Wang, Qin; Wei, Xuetuan; Nomura, Christopher T.; Chen, Shouwen

Year: 2017

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-017-0688-7

Paper Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

Camattari, Andrea; Goh, Amelia; Yip, Lian Yee et al. · 2016 · Microbial Cell Factories · DOI

Full title: Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

Authors: Camattari, Andrea; Goh, Amelia; Yip, Lian Yee; Tan, Andy Hee‐Meng; Ng, Sze Wai; Tran, Anthony H.; Liu, Gaowen; Liachko, Ivan; Dunham, Maitreya J.; Rancati, Giulia

Year: 2016

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-016-0540-5

Paper Directed evolution and secretory expression of a pyrethroid-hydrolyzing esterase with enhanced catalytic activity and thermostability

Liu, Xiaolong; Liang, Mingjun; Liu, Yuhuan et al. · 2017 · Microbial Cell Factories · DOI

Full title: Directed evolution and secretory expression of a pyrethroid-hydrolyzing esterase with enhanced catalytic activity and thermostability

Authors: Liu, Xiaolong; Liang, Mingjun; Liu, Yuhuan; Fan, Xinjiong

Year: 2017

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-017-0698-5

Paper Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris

J. Moser; R. Prielhofer; Samuel M. Gerner et al. · 2017 · Microbial Cell Factories · DOI

Full title: Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris

Authors: J. Moser; R. Prielhofer; Samuel M. Gerner; A. Graf; I. Wilson; D. Mattanovich; Martin Dragosits

Year: 2017

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-017-0661-5

Paper Genetic basis for hyper production of hyaluronic acid in natural and engineered microorganisms

Oliveira, Juliana Davies de; Carvalho, Lucas Silva; Gomes, Antônio Milton Vieira et al. · 2016 · Microbial Cell Factories · DOI

Full title: Genetic basis for hyper production of hyaluronic acid in natural and engineered microorganisms

Authors: Oliveira, Juliana Davies de; Carvalho, Lucas Silva; Gomes, Antônio Milton Vieira; Queiroz, Lúcio; Magalhães, Beatriz Simas; Parachin, Nádia Skorupa

Year: 2016

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-016-0517-4

Paper Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica

Dulermo, Rémi; Brunel, François; Dulermo, Thierry et al. · 2017 · Microbial Cell Factories · DOI

Full title: Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica

Authors: Dulermo, Rémi; Brunel, François; Dulermo, Thierry; Ledesma‐Amaro, Rodrigo; Vion, Jérémy; Trassaert, Marion; Thomas, Stéphane; Nicaud, Jean‐Marc; Leplat, Christophe

Year: 2017

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-017-0647-3

Paper New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica

Trassaert, Marion; Vandermies, Marie; Carly, Frédéric et al. · 2017 · Microbial Cell Factories · DOI

Full title: New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica

Authors: Trassaert, Marion; Vandermies, Marie; Carly, Frédéric; Denies, Olivia; Thomas, Stéphane; Fickers, Patrick; Nicaud, Jean‐Marc

Year: 2017

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-017-0755-0

Paper Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures

Burgard, Jonas; Valli, Minoska; Graf, Alexandra B. et al. · 2017 · Microbial Cell Factories · DOI

Full title: Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures

Authors: Burgard, Jonas; Valli, Minoska; Graf, Alexandra B.; Gasser, Brigitte; Mattanovich, Diethard

Year: 2017

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-017-0730-9

Paper A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris

Rajamanickam, Vignesh; Metzger, Karl; Schmid, Christian et al. · 2017 · Microbial Cell Factories · DOI

Full title: A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris

Authors: Rajamanickam, Vignesh; Metzger, Karl; Schmid, Christian; Spadiut, Oliver

Year: 2017

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-017-0768-8

Paper Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker

Betancur, Maritza Ocampo; Reis, Viviane Castelo Branco; Nicola, André Moraes et al. · 2017 · Microbial Cell Factories · DOI

Full title: Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker

Authors: Betancur, Maritza Ocampo; Reis, Viviane Castelo Branco; Nicola, André Moraes; Marco, Janice Lisboa De; Moraes, Lídia Maria Pepe de; Torres, Fernando Araripe Gonçalves

Year: 2017

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-017-0715-8

Paper The effect of hypoxia on the lipidome of recombinant Pichia pastoris

Núria Adelantado; P. Tarazona; Karlheinz Grillitsch et al. · 2017 · Microbial Cell Factories · DOI

Full title: The effect of hypoxia on the lipidome of recombinant Pichia pastoris

Authors: Núria Adelantado; P. Tarazona; Karlheinz Grillitsch; Xavier Garcia-Ortega; Sergi Monforte; F. Valero; I. Feussner; G. Daum; P. Ferrer

Year: 2017

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-017-0699-4

Paper Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems

Ma, Su; Preims, Marita; Piumi, François et al. · 2017 · Microbial Cell Factories · DOI

Full title: Molecular and catalytic properties of fungal extracellular cellobiose dehydrogenase produced in prokaryotic and eukaryotic expression systems

Authors: Ma, Su; Preims, Marita; Piumi, François; Kappel, Lisa; Seiboth, Bernhard; Record, Éric; Kracher, Daniel; Ludwig, Roland

Year: 2017

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-017-0653-5

Paper Fate of the UPR marker protein Kar2/Bip and autophagic processes in fed-batch cultures of secretory insulin precursor producing Pichia pastoris

G. Roth; Ana Letícia Vanz; H. Lünsdorf et al. · 2018 · Microbial Cell Factories · DOI

Full title: Fate of the UPR marker protein Kar2/Bip and autophagic processes in fed-batch cultures of secretory insulin precursor producing Pichia pastoris

Authors: G. Roth; Ana Letícia Vanz; H. Lünsdorf; M. Nimtz; U. Rinas

Year: 2018

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-018-0970-3

Paper An improved secretion signal enhances the secretion of model proteins from Pichia pastoris

Barrero, Juan J.; Casler, Jason C.; Valero, Francisco et al. · 2018 · Microbial Cell Factories · DOI

Full title: An improved secretion signal enhances the secretion of model proteins from Pichia pastoris

Authors: Barrero, Juan J.; Casler, Jason C.; Valero, Francisco; Ferrer, Pau; Glick, Benjamin S.

Year: 2018

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-018-1009-5

Paper Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

Maršálek, Lukáš; Puxbaum, Verena; Buchetics, Markus et al. · 2019 · Microbial Cell Factories · DOI

Full title: Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

Authors: Maršálek, Lukáš; Puxbaum, Verena; Buchetics, Markus; Mattanovich, Diethard; Gasser, Brigitte

Year: 2019

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-019-1155-4

Paper Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity

Kidibule, Peter Elias; Santos‐Moriano, Paloma; Jiménez‐Ortega, Elena et al. · 2018 · Microbial Cell Factories · DOI

Full title: Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity

Authors: Kidibule, Peter Elias; Santos‐Moriano, Paloma; Jiménez‐Ortega, Elena; Ramirez-Escudero, M.; Limón, M. Carmen; Remacha, Miguel; Plou, Francisco J.; Sanz‐Aparicio, J.; Fernández‐Lobato, María

Year: 2018

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-018-0895-x

Paper Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein

Daniels, Wouter; Bouvin, Jeroen; Busche, Tobias et al. · 2018 · Microbial Cell Factories · DOI

Full title: Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein

Authors: Daniels, Wouter; Bouvin, Jeroen; Busche, Tobias; Rückert, Christian; Simoens, Kenneth; Karamanou, Spyridoula; Mellaert, Lieve Van; Friðjónsson, Ólafur H.; Nicolaı̈, Bart; Economou, Anastassios; Kalinowski, Jörn; Anné, Jozef; Bernaerts, Kristel

Year: 2018

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-018-1040-6

Paper Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison

Pekarsky, Alexander; Veiter, Lukas; Rajamanickam, Vignesh et al. · 2018 · Microbial Cell Factories · DOI

Full title: Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison

Authors: Pekarsky, Alexander; Veiter, Lukas; Rajamanickam, Vignesh; Herwig, Christoph; Grünwald‐Gruber, Clemens; Altmann, Friedrich; Spadiut, Oliver

Year: 2018

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-018-1032-6

Paper Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage

Garrigós‐Martínez, Javier; Nieto‐Taype, Miguel Angel; Gasset-Franch, Arnau et al. · 2019 · Microbial Cell Factories · DOI

Full title: Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage

Authors: Garrigós‐Martínez, Javier; Nieto‐Taype, Miguel Angel; Gasset-Franch, Arnau; Montesinos, José Luis González; Garcia‐Ortega, Xavier; Valero, Francisco

Year: 2019

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-019-1240-8

Paper A glucotolerant β-glucosidase from the fungus Talaromyces amestolkiae and its conversion into a glycosynthase for glycosylation of phenolic compounds

Méndez-Líter, Juan A.; Nieto‐Domínguez, Manuel; Toro, Beatriz Fernández de et al. · 2020 · Microbial Cell Factories · DOI

Full title: A glucotolerant β-glucosidase from the fungus Talaromyces amestolkiae and its conversion into a glycosynthase for glycosylation of phenolic compounds

Authors: Méndez-Líter, Juan A.; Nieto‐Domínguez, Manuel; Toro, Beatriz Fernández de; Santana, Andrés G.; Prieto, Alicia; Asensio, Juan Luis; Cañada, F. Javier; Eugenio, Laura I. de; Martı́nez, Marı́a Jesús

Year: 2020

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-020-01386-1

Paper CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

Liu, Qi; Shi, Xiaona; Song, Lili et al. · 2019 · Microbial Cell Factories · DOI

Full title: CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

Authors: Liu, Qi; Shi, Xiaona; Song, Lili; Liu, Haifeng; Zhou, Xiangshan; Wang, Qiyao; Zhang, Yuanxing; Cai, Menghao

Year: 2019

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-019-1194-x

Paper Construction of a novel MK-4 biosynthetic pathway in Pichia pastoris through heterologous expression of HsUBIAD1

Sun, Xiaowen; Liu, Hui; Wang, Peng et al. · 2019 · Microbial Cell Factories · DOI

Full title: Construction of a novel MK-4 biosynthetic pathway in Pichia pastoris through heterologous expression of HsUBIAD1

Authors: Sun, Xiaowen; Liu, Hui; Wang, Peng; Wang, Li; Ni, Wenfeng; Yang, Qiang; Wang, Han; Tang, Hengfang; Zhao, Genhai; Zheng, Zhiming

Year: 2019

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-019-1215-9

Paper Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in process conditions

Park, Young Kyoung; Vandermies, Marie; Soudier, Paul et al. · 2019 · Microbial Cell Factories · DOI

Full title: Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in process conditions

Authors: Park, Young Kyoung; Vandermies, Marie; Soudier, Paul; Telek, Samuel; Thomas, Stéphane; Nicaud, Jean‐Marc; Fickers, Patrick

Year: 2019

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-019-1218-6

Paper Enhanced extracellular expression of Bacillus stearothermophilus α-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and α-amylase mutant selection

Yao, Dongbang; Su, Lingqia; Li, Na et al. · 2019 · Microbial Cell Factories · DOI

Full title: Enhanced extracellular expression of Bacillus stearothermophilus α-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and α-amylase mutant selection

Authors: Yao, Dongbang; Su, Lingqia; Li, Na; Wu, Jing

Year: 2019

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-019-1119-8

Paper Improving production of Streptomyces griseus trypsin for enzymatic processing of insulin precursor.

Zhang, Yunfeng; Liang, Qixing; Zhang, Chuanzhi et al. · 2020 · Microbial cell factories · DOI

Full title: Improving production of Streptomyces griseus trypsin for enzymatic processing of insulin precursor.

Authors: Zhang, Yunfeng; Liang, Qixing; Zhang, Chuanzhi; Zhang, Juan; Du, Guocheng; Kang, Zhen

Year: 2020

Venue: Microbial cell factories

DOI: https://doi.org/10.1186/s12934-020-01338-9

Paper Homogeneous production and characterization of recombinant N-GlcNAc-protein in Pichia pastoris

Wang, Shengjun; Rong, Yongheng; Wang, Yaoguang et al. · 2020 · Microbial Cell Factories · DOI

Full title: Homogeneous production and characterization of recombinant N-GlcNAc-protein in Pichia pastoris

Authors: Wang, Shengjun; Rong, Yongheng; Wang, Yaoguang; Kong, Decai; Wang, Peng George; Chen, Min; Kong, Yun

Year: 2020

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-020-1280-0

Paper The SARS-CoV-2 receptor-binding domain expressed in Pichia pastoris as a candidate vaccine antigen

Limonta‐Fernández, Miladys; Chinea-Santiago, Glay; Martin, A.M. et al. · 2021 · DOI

Full title: The SARS-CoV-2 receptor-binding domain expressed in Pichia pastoris as a candidate vaccine antigen

Authors: Limonta‐Fernández, Miladys; Chinea-Santiago, Glay; Martin, A.M.; Gonzalez-Roche, Diamile; Béquet‐Romero, Mónica; Márquez, Gabriel; González-Moya, Isabel; Canaan-Haden-Ayala, Camila; Cabrales, Ania; Espinosa, Luis Ariel; Ramos, Yassel; Andújar, Iván; González, Luis Javier; Iglesia, Mariela Pérez de la; Zamora-Sánchez, Jesús; Cruz-Sui, Otto; Lemos, Gilda; Cabrera, Gleysin; Valdés-Hernández, Jorge; Martínez-Díaz, Eduardo; Pimentel-Vázquez, Eulogio; Ayala‐Ávila, Marta; Guillén, Gerardo

Year: 2021

DOI: https://doi.org/10.1101/2021.06.29.21259605

Paper Improving the heterologous production of fungal peroxygenases through an episomal Pichia pastoris promoter and signal peptide shuffling system

Pascal Püllmann; M. Weissenborn · 2020 · bioRxiv · DOI

Full title: Improving the heterologous production of fungal peroxygenases through an episomal Pichia pastoris promoter and signal peptide shuffling system

Authors: Pascal Püllmann; M. Weissenborn

Year: 2020

Venue: bioRxiv

DOI: https://doi.org/10.1101/2020.12.23.424034

Paper OPENPichia: building a free-to-operate Komagataella phaffii protein expression toolkit

Dries Van Herpe; R. Vanluchene; Kristof Vandewalle et al. · 2022 · bioRxiv · DOI

Full title: OPENPichia: building a free-to-operate Komagataella phaffii protein expression toolkit

Authors: Dries Van Herpe; R. Vanluchene; Kristof Vandewalle; S. Vanmarcke; Elise Wyseure; Berre Van Moer; H. Eeckhaut; D. Fijałkowska; Hendrik Grootaert; Chiara Lonigro; L. Meuris; Gitte Michielsen; Justine Naessens; Charlotte Roels; Loes van Schie; R. De Rycke; Michiel De Bruyne; P. Borghgraef; K. Claes; N. Callewaert

Year: 2022

Venue: bioRxiv

DOI: https://doi.org/10.1101/2022.12.13.519130

Paper Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing

Liang, Shuli; Wang, Bin; Li, Pan et al. · 2012 · BMC Genomics · DOI

Full title: Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing

Authors: Liang, Shuli; Wang, Bin; Li, Pan; Ye, Yanrui; He, Minghui; Han, Shuangyan; Zheng, Suiping; Wang, Xiaoning; Lin, Ying

Year: 2012

Venue: BMC Genomics

DOI: https://doi.org/10.1186/1471-2164-13-738

Paper Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays

A. Graf; Brigitte Gasser; Martin Dragosits et al. · 2008 · BMC Genomics · DOI

Full title: Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays

Authors: A. Graf; Brigitte Gasser; Martin Dragosits; M. Sauer; G. Leparc; Thomas Tüchler; David P. Kreil; D. Mattanovich

Year: 2008

Venue: BMC Genomics

DOI: https://doi.org/10.1186/1471-2164-9-390

Paper Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1

Ravin, Nikolai V.; Eldarov, Michael A.; Kadnikov, Vitaly V. et al. · 2013 · BMC Genomics · DOI

Full title: Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1

Authors: Ravin, Nikolai V.; Eldarov, Michael A.; Kadnikov, Vitaly V.; Beletsky, Alexey V.; Schneider, Jessica; Mardanova, Eugenia S.; Smekalova, Elena M.; Zvereva, Maria I.; Донцова, О. А.; Mardanov, Andrey V.; Skryabin, K. G.

Year: 2013

Venue: BMC Genomics

DOI: https://doi.org/10.1186/1471-2164-14-837

Paper Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions

Brigitte Gasser; Michael Maurer; J. Rautio et al. · 2007 · BMC Genomics · DOI

Full title: Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions

Authors: Brigitte Gasser; Michael Maurer; J. Rautio; M. Sauer; A. Bhattacharyya; M. Saloheimo; M. Penttilä; D. Mattanovich

Year: 2007

Venue: BMC Genomics

DOI: https://doi.org/10.1186/1471-2164-8-179

Paper “Fight-flight-or-freeze” – how Yarrowia lipolytica responds to stress at molecular level?

Celińska, Ewelina · 2022 · Applied Microbiology and Biotechnology · DOI

Full title: “Fight-flight-or-freeze” – how Yarrowia lipolytica responds to stress at molecular level?

Authors: Celińska, Ewelina

Year: 2022

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-022-11934-x

Paper A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases

Püllmann, Pascal; Knorrscheidt, Anja; Münch, Judith et al. · 2021 · Communications Biology · DOI

Full title: A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases

Authors: Püllmann, Pascal; Knorrscheidt, Anja; Münch, Judith; Palme, Paul R.; Hoehenwarter, Wolfgang; Marillonnet, Sylvestre; Alcalde, Miguel; Westermann, Bernhard; Weissenborn, Martin J.

Year: 2021

Venue: Communications Biology

DOI: https://doi.org/10.1038/s42003-021-02076-3

Paper Expressing Cloned Genes for Protein Production, Purification, and Analysis

Kielkopf, Clara L.; Bauer, William R.; Urbatsch, Ina L. · 2020 · Cold Spring Harbor Protocols · DOI

Full title: Expressing Cloned Genes for Protein Production, Purification, and Analysis

Authors: Kielkopf, Clara L.; Bauer, William R.; Urbatsch, Ina L.

Year: 2020

Venue: Cold Spring Harbor Protocols

DOI: https://doi.org/10.1101/pdb.top102129

Paper Microbial cellulase production and its potential application for textile industries

Korsa, Gamachis; Konwarh, Rocktotpal; Masi, Chandran et al. · 2023 · Annals of Microbiology · DOI

Full title: Microbial cellulase production and its potential application for textile industries

Authors: Korsa, Gamachis; Konwarh, Rocktotpal; Masi, Chandran; Ayele, Abate; Haile, Setegn

Year: 2023

Venue: Annals of Microbiology

DOI: https://doi.org/10.1186/s13213-023-01715-w

Paper A detailed overview of xylanases: an emerging biomolecule for current and future prospective

Bhardwaj, Nisha; Kumar, Bikash; Verma, Pradeep · 2019 · Bioresources and Bioprocessing · DOI

Full title: A detailed overview of xylanases: an emerging biomolecule for current and future prospective

Authors: Bhardwaj, Nisha; Kumar, Bikash; Verma, Pradeep

Year: 2019

Venue: Bioresources and Bioprocessing

DOI: https://doi.org/10.1186/s40643-019-0276-2

Paper Current perspective on production and applications of microbial cellulases: a review

Bhardwaj, Nisha; Kumar, Bikash; Agrawal, Komal et al. · 2021 · Bioresources and Bioprocessing · DOI

Full title: Current perspective on production and applications of microbial cellulases: a review

Authors: Bhardwaj, Nisha; Kumar, Bikash; Agrawal, Komal; Verma, Pradeep

Year: 2021

Venue: Bioresources and Bioprocessing

DOI: https://doi.org/10.1186/s40643-021-00447-6

Paper Pichia pastoris as a cell factory for the secreted production of tunable collagen-inspired gel-forming proteins

Cicera Elaine da Silva · 2013 · DOI

Full title: Pichia pastoris as a cell factory for the secreted production of tunable collagen-inspired gel-forming proteins

Authors: Cicera Elaine da Silva

Year: 2013

DOI: https://doi.org/10.18174/242734

Paper Metabolic studies on Schizosaccharomyces pombe for improved protein secretion

Tobias Klein · 2013 · Prof. Dr. · DOI

Full title: Metabolic studies on Schizosaccharomyces pombe for improved protein secretion

Authors: Tobias Klein

Year: 2013

Venue: Prof. Dr.

DOI: https://doi.org/10.22028/d291-22954

Paper Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Tripathi, Nagesh K.; Shrivastava, Ambuj · 2019 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Authors: Tripathi, Nagesh K.; Shrivastava, Ambuj

Year: 2019

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2019.00420

Paper Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory

Miguel Angel Nieto-Taype; Xavier Garcia-Ortega; J. Albiol et al. · 2020 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory

Authors: Miguel Angel Nieto-Taype; Xavier Garcia-Ortega; J. Albiol; José Luis Montesinos-Seguí; F. Valero

Year: 2020

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2020.00632

Paper Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins

Rivera‐de‐Torre, Esperanza; Rimbault, Charlotte; Jenkins, Timothy P. et al. · 2022 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins

Authors: Rivera‐de‐Torre, Esperanza; Rimbault, Charlotte; Jenkins, Timothy P.; Sørensen, Christoffer V.; Damsbo, Anna; Saez, Natalie J.; Duhoo, Yoan; Hackney, Celeste Menuet; Ellgaard, Lars; Laustsen, Andreas H.

Year: 2022

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2021.811905

Paper Improving Immunotherapy Through Glycodesign

Buettner, Matthew J.; Shah, Sagar; Saeui, Christopher T. et al. · 2018 · Frontiers in Immunology · DOI

Full title: Improving Immunotherapy Through Glycodesign

Authors: Buettner, Matthew J.; Shah, Sagar; Saeui, Christopher T.; Ariss, Ryan; Yarema, Kevin J.

Year: 2018

Venue: Frontiers in Immunology

DOI: https://doi.org/10.3389/fimmu.2018.02485

Paper Expression of Recombinant Antibodies

Frenzel, André; Hust, Michael; Schirrmann, Thomas · 2013 · Frontiers in Immunology · DOI

Full title: Expression of Recombinant Antibodies

Authors: Frenzel, André; Hust, Michael; Schirrmann, Thomas

Year: 2013

Venue: Frontiers in Immunology

DOI: https://doi.org/10.3389/fimmu.2013.00217

Paper Technological Microbiology: Development and Applications

Vitorino, Luciana Cristina; Bessa, Layara Alexandre · 2017 · Frontiers in Microbiology · DOI

Full title: Technological Microbiology: Development and Applications

Authors: Vitorino, Luciana Cristina; Bessa, Layara Alexandre

Year: 2017

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2017.00827

Paper Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

Dubey, Manish Kumar; Zehra, Andleeb; Aamir, Mohd et al. · 2017 · Frontiers in Microbiology · DOI

Full title: Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

Authors: Dubey, Manish Kumar; Zehra, Andleeb; Aamir, Mohd; Meena, Mukesh; Ahirwal, Laxmi; Singh, Siddhartha; Shukla, Shruti; Upadhyay, Ram Sanmukh; Bueno‐Marí, Rubén; Bajpai, Vivek K.

Year: 2017

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2017.01032

Paper A Review of the Microbial Production of Bioactive Natural Products and Biologics

Pham, Janette V.; Yilma, Mariamawit A.; Feliz, Adriana et al. · 2019 · Frontiers in Microbiology · DOI

Full title: A Review of the Microbial Production of Bioactive Natural Products and Biologics

Authors: Pham, Janette V.; Yilma, Mariamawit A.; Feliz, Adriana; Majid, Murtadha T.; Maffetone, Nicholas; Walker, Jorge R.; Kim, Eunji; Cho, Hyo Je; Reynolds, Jared M.; Song, Myoung‐Chong; Park, Sung Ryeol; Yoon, Yeo Joon

Year: 2019

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2019.01404

Paper Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry

F. J. Contesini; M. G. Davanço; G. Borin et al. · 2020 · Catalysts · DOI

Full title: Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry

Authors: F. J. Contesini; M. G. Davanço; G. Borin; Katherina García Vanegas; João Pedro Gonçalves Cirino; R. Melo; U. Mortensen; K. Hildén; D. R. Campos; P. Carvalho

Year: 2020

Venue: Catalysts

DOI: https://doi.org/10.3390/catal10091032

Paper Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications

Borrelli, Grazia Maria; Trono, Daniela · 2015 · International Journal of Molecular Sciences · DOI

Full title: Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications

Authors: Borrelli, Grazia Maria; Trono, Daniela

Year: 2015

Venue: International Journal of Molecular Sciences

DOI: https://doi.org/10.3390/ijms160920774

Paper Engineering and Expression Strategies for Optimization of L-Asparaginase Development and Production

A. N. Shishparenok; Y. Gladilina; D. Zhdanov · 2023 · International Journal of Molecular Sciences · DOI

Full title: Engineering and Expression Strategies for Optimization of L-Asparaginase Development and Production

Authors: A. N. Shishparenok; Y. Gladilina; D. Zhdanov

Year: 2023

Venue: International Journal of Molecular Sciences

DOI: https://doi.org/10.3390/ijms242015220

Paper Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories Improvement

Madzak, Catherine · 2021 · Journal of Fungi · DOI

Full title: Yarrowia lipolytica Strains and Their Biotechnological Applications: How Natural Biodiversity and Metabolic Engineering Could Contribute to Cell Factories Improvement

Authors: Madzak, Catherine

Year: 2021

Venue: Journal of Fungi

DOI: https://doi.org/10.3390/jof7070548

Paper Strategies for the Development of Industrial Fungal Producing Strains

Salazar-Cerezo, Sonia; Vries, Ronald P. de; Garrigues, Sandra · 2023 · Journal of Fungi · DOI

Full title: Strategies for the Development of Industrial Fungal Producing Strains

Authors: Salazar-Cerezo, Sonia; Vries, Ronald P. de; Garrigues, Sandra

Year: 2023

Venue: Journal of Fungi

DOI: https://doi.org/10.3390/jof9080834

Paper Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry

Хлебодарова, Т. М.; Bogacheva, N. V.; Zadorozhny, A. V. et al. · 2024 · Microorganisms · DOI

Full title: Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry

Authors: Хлебодарова, Т. М.; Bogacheva, N. V.; Zadorozhny, A. V.; Bryanskaya, A. V.; Vasilieva, Asya R.; Chesnokov, Danil Olegovich; Pavlova, Elena I.; Peltek, S. E.

Year: 2024

Venue: Microorganisms

DOI: https://doi.org/10.3390/microorganisms12020346

Paper A Review of Chondroitin Sulfate’s Preparation, Properties, Functions, and Applications

Shen, Qingshan; Guo, Yujie; Wang, Kangyu et al. · 2023 · Molecules · DOI

Full title: A Review of Chondroitin Sulfate’s Preparation, Properties, Functions, and Applications

Authors: Shen, Qingshan; Guo, Yujie; Wang, Kangyu; Zhang, Chunhui; Ma, Yanli

Year: 2023

Venue: Molecules

DOI: https://doi.org/10.3390/molecules28207093

Paper Hyperthermostable Thermotoga maritima xylanase XYN10B shows high activity at high temperatures in the presence of biomass-dissolving hydrophilic ionic liquids

Yu, Tianyi; Anbarasan, Sasikala; Wang, Yawei et al. · 2016 · Extremophiles · DOI

Full title: Hyperthermostable Thermotoga maritima xylanase XYN10B shows high activity at high temperatures in the presence of biomass-dissolving hydrophilic ionic liquids

Authors: Yu, Tianyi; Anbarasan, Sasikala; Wang, Yawei; Telli, Kübra; Aslan, Aşkın Sevinç; Su, Zhengding; Zhou, Yin; Zhang, Li; Iivonen, Piia; Havukainen, Sami; Mentunen, Tero; Hummel, Michael; Sixta, Herbert; Bi̇nay, Barış; Turunen, Ossi; Xiong, Hairong

Year: 2016

Venue: Extremophiles

DOI: https://doi.org/10.1007/s00792-016-0841-y

Paper Improving Enzymes for Biomass Conversion: A Basic Research Perspective

Banerjee, Goutami; Scott‐Craig, John S.; Walton, Jonathan D. · 2010 · BioEnergy Research · DOI

Full title: Improving Enzymes for Biomass Conversion: A Basic Research Perspective

Authors: Banerjee, Goutami; Scott‐Craig, John S.; Walton, Jonathan D.

Year: 2010

Venue: BioEnergy Research

DOI: https://doi.org/10.1007/s12155-009-9067-5

Paper Assay and heterologous expression in Pichia pastoris of plant cell wall type-II membrane anchored glycosyltransferases

Petersen, Bent Larsen; Egelund, Jack; Damager, Iben et al. · 2009 · Glycoconjugate Journal · DOI

Full title: Assay and heterologous expression in Pichia pastoris of plant cell wall type-II membrane anchored glycosyltransferases

Authors: Petersen, Bent Larsen; Egelund, Jack; Damager, Iben; Faber, Kirsten; Jensen, Jacob Krüger; Yang, Zhang; Bennett, Eric; Scheller, Henrik Vibe; Ulvskov, Peter

Year: 2009

Venue: Glycoconjugate Journal

DOI: https://doi.org/10.1007/s10719-009-9242-0

Paper Aspergillus as a multi-purpose cell factory: current status and perspectives

Meyer, Vera; Wu, Bo; Ram, Arthur F. J. · 2010 · Biotechnology Letters · DOI

Full title: Aspergillus as a multi-purpose cell factory: current status and perspectives

Authors: Meyer, Vera; Wu, Bo; Ram, Arthur F. J.

Year: 2010

Venue: Biotechnology Letters

DOI: https://doi.org/10.1007/s10529-010-0473-8

Paper Microbial xylanases and their industrial application in pulp and paper biobleaching: a review

Walia, Abhishek; Guleria, Shiwani; Mehta, Preeti et al. · 2017 · 3 Biotech · DOI

Full title: Microbial xylanases and their industrial application in pulp and paper biobleaching: a review

Authors: Walia, Abhishek; Guleria, Shiwani; Mehta, Preeti; Chauhan, Anjali; Parkash, Jyoti

Year: 2017

Venue: 3 Biotech

DOI: https://doi.org/10.1007/s13205-016-0584-6

Paper The recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fusarium, Colletotrichum and Phakopsora

Lacerda, Ariane Ferreira; Sarto, Rafael Perseghini Del; Silva, Marília Santos et al. · 2016 · 3 Biotech · DOI

Full title: The recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fusarium, Colletotrichum and Phakopsora

Authors: Lacerda, Ariane Ferreira; Sarto, Rafael Perseghini Del; Silva, Marília Santos; Vasconcelos, Érico Augusto Rosas de; Coelho, Roberta Ramos; Santos, Vanessa Olinto dos; Godoy, C. V.; Seixas, C. D. S.; Silva, Maria Cristina Mattar da; Grossi‐de‐Sá, Maria Fátima

Year: 2016

Venue: 3 Biotech

DOI: https://doi.org/10.1007/s13205-015-0320-7

Paper From small to large-scale: a review of recombinant spider silk and collagen bioproduction

Delgado‐Gomes, Vasco; Salgueiro, Sancha P. · 2022 · Discover Materials · DOI

Full title: From small to large-scale: a review of recombinant spider silk and collagen bioproduction

Authors: Delgado‐Gomes, Vasco; Salgueiro, Sancha P.

Year: 2022

Venue: Discover Materials

DOI: https://doi.org/10.1007/s43939-022-00024-4

Paper Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii.

Alva, Troy R; Riera, Melanie; Chartron, Justin W · 2021 · Microbial cell factories · DOI

Full title: Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii.

Authors: Alva, Troy R; Riera, Melanie; Chartron, Justin W

Year: 2021

Venue: Microbial cell factories

DOI: https://doi.org/10.1186/s12934-020-01489-9

Paper Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc

Song, Wen; Zhang, Nuo; Yang, Mo et al. · 2020 · Microbial Cell Factories · DOI

Full title: Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc

Authors: Song, Wen; Zhang, Nuo; Yang, Mo; Zhou, Yuling; He, Nisha; Zhang, Guimin

Year: 2020

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-020-01440-y

Paper Engineering cofactor metabolism for improved protein and glucoamylase production in Aspergillus niger

Sui, Yufei; Schütze, Tabea; Ouyang, Liming et al. · 2020 · Microbial Cell Factories · DOI

Full title: Engineering cofactor metabolism for improved protein and glucoamylase production in Aspergillus niger

Authors: Sui, Yufei; Schütze, Tabea; Ouyang, Liming; Lu, Hongzhong; Liu, Peng; Xiao, Xianzun; Qi, Jie; Zhuang, Yingping; Meyer, Vera

Year: 2020

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-020-01450-w

Paper Codon usage bias regulates gene expression and protein conformation in yeast expression system P. pastoris

Xu, Yichun; Liu, Kunshan; Han, Yu et al. · 2021 · Microbial Cell Factories · DOI

Full title: Codon usage bias regulates gene expression and protein conformation in yeast expression system P. pastoris

Authors: Xu, Yichun; Liu, Kunshan; Han, Yu; Xing, Yanzi; Zhang, Yuanxing; Yang, Qiuying; Zhou, Mian

Year: 2021

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-021-01580-9

Paper Heterologous expression of Phanerochaete chrysosporium cellobiose dehydrogenase in Trichoderma reesei

Wohlschlager, Lena; Csarman, Florian; Chang, Hucheng et al. · 2021 · Microbial Cell Factories · DOI

Full title: Heterologous expression of Phanerochaete chrysosporium cellobiose dehydrogenase in Trichoderma reesei

Authors: Wohlschlager, Lena; Csarman, Florian; Chang, Hucheng; Fitz, Elisabeth; Seiboth, Bernhard; Ludwig, Roland

Year: 2021

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-020-01492-0

Paper Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene

Aggarwal, Sakshi; Mishra, Saroj · 2020 · Microbial Cell Factories · DOI

Full title: Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene

Authors: Aggarwal, Sakshi; Mishra, Saroj

Year: 2020

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-020-01460-8

Paper Genome-based engineering of ligninolytic enzymes in fungi

Asemoloye, Michael Dare; Marchisio, Mario Andrea; Gupta, Vijai Kumar et al. · 2021 · Microbial Cell Factories · DOI

Full title: Genome-based engineering of ligninolytic enzymes in fungi

Authors: Asemoloye, Michael Dare; Marchisio, Mario Andrea; Gupta, Vijai Kumar; Pecoraro, Lorenzo

Year: 2021

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-021-01510-9

Paper Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris

Garrigós‐Martínez, Javier; Vuoristo, Kiira S.; Nieto‐Taype, Miguel Angel et al. · 2021 · Microbial Cell Factories · DOI

Full title: Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris

Authors: Garrigós‐Martínez, Javier; Vuoristo, Kiira S.; Nieto‐Taype, Miguel Angel; Tähtiharju, Juha; Uusitalo, Jaana; Tukiainen, Pauliina; Schmid, Christian; Tolstorukov, Ilya; Madden, Knut; Penttilä, Merja; Montesinos, José Luis González; Valero, Francisco; Glieder, Anton; Garcia‐Ortega, Xavier

Year: 2021

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-021-01564-9

Paper Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up

A. Madhavan; K. Arun; R. Sindhu et al. · 2021 · Microbial Cell Factories · DOI

Full title: Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up

Authors: A. Madhavan; K. Arun; R. Sindhu; Jayaraman Krishnamoorthy; R. Reshmy; Ranjna Sirohi; Arivalagan Pugazhendi; M. Awasthi; G. Szakács; P. Binod

Year: 2021

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-021-01617-z

Paper Screening and evaluation of the strong endogenous promoters in Pichia pastoris

Weiwang Dou; Quanchao Zhu; Meihua Zhang et al. · 2021 · Microbial Cell Factories · DOI

Full title: Screening and evaluation of the strong endogenous promoters in Pichia pastoris

Authors: Weiwang Dou; Quanchao Zhu; Meihua Zhang; Zuyuan Jia; W. Guan

Year: 2021

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-021-01648-6

Paper The future of recombinant host defense peptides

Roca-Pinilla, Ramon; Lisowski, Leszek; Arı́s, Anna et al. · 2022 · Microbial Cell Factories · DOI

Full title: The future of recombinant host defense peptides

Authors: Roca-Pinilla, Ramon; Lisowski, Leszek; Arı́s, Anna; García‐Fruitós, Elena

Year: 2022

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-022-01991-2

Paper Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9

Javier Garrigós-Martínez; A. Weninger; José Luis Montesinos-Seguí et al. · 2021 · Microbial Cell Factories · DOI

Full title: Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9

Authors: Javier Garrigós-Martínez; A. Weninger; José Luis Montesinos-Seguí; Christian Schmid; F. Valero; Claudia Rinnofner; A. Glieder; Xavier Garcia-Ortega

Year: 2021

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-021-01577-4

Paper Expression of antibody fragments in Saccharomyces cerevisiae strains evolved for enhanced protein secretion

Wang, Yanyan; Li, Xiaowei; Chen, Xin et al. · 2021 · Microbial Cell Factories · DOI

Full title: Expression of antibody fragments in Saccharomyces cerevisiae strains evolved for enhanced protein secretion

Authors: Wang, Yanyan; Li, Xiaowei; Chen, Xin; Nielsen, Jens; Petranović, Dina; Siewers, Verena

Year: 2021

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-021-01624-0

Paper Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris.

Navone, Laura; Vogl, Thomas; Luangthongkam, Pawarisa et al. · 2021 · Microbial cell factories · DOI

Full title: Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris.

Authors: Navone, Laura; Vogl, Thomas; Luangthongkam, Pawarisa; Blinco, Jo-Anne; Luna-Flores, Carlos; Chen, Xiaojing; von Hellens, Juhani; Speight, Robert

Year: 2021

Venue: Microbial cell factories

DOI: https://doi.org/10.1186/s12934-020-01499-7

Paper Codon pair optimization (CPO): a software tool for synthetic gene design based on codon pair bias to improve the expression of recombinant proteins in Pichia pastoris

Huang, Yide; Lin, Ting; Lu, Lingfang et al. · 2021 · Microbial Cell Factories · DOI

Full title: Codon pair optimization (CPO): a software tool for synthetic gene design based on codon pair bias to improve the expression of recombinant proteins in Pichia pastoris

Authors: Huang, Yide; Lin, Ting; Lu, Lingfang; Cai, Fan; Lin, Jie; Jiang, Yue; Lin, Yao

Year: 2021

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-021-01696-y

Paper Design and heterologous expression of a novel dimeric LL37 variant in Pichia pastoris

Zhan, Na; Zhang, Licong; Yang, Hong et al. · 2021 · Microbial Cell Factories · DOI

Full title: Design and heterologous expression of a novel dimeric LL37 variant in Pichia pastoris

Authors: Zhan, Na; Zhang, Licong; Yang, Hong; Zheng, Yalan; Wei, Xinke; Wang, Jiajun; Shan, Anshan

Year: 2021

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-021-01635-x

Paper Engineering a carbohydrate-binding module to increase the expression level of glucoamylase in Pichia pastoris

Lige Tong; Huo-qing Huang; Jie Zheng et al. · 2022 · Microbial Cell Factories · DOI

Full title: Engineering a carbohydrate-binding module to increase the expression level of glucoamylase in Pichia pastoris

Authors: Lige Tong; Huo-qing Huang; Jie Zheng; Xiao Wang; Yingguo Bai; Xiaolu Wang; Yuan Wang; T. Tu; B. Yao; Xing Qin; Huiying Luo

Year: 2022

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-022-01833-1

Paper Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions

Groeve, Manu De; Laukens, Bram; Schotte, Peter · 2023 · Microbial Cell Factories · DOI

Full title: Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions

Authors: Groeve, Manu De; Laukens, Bram; Schotte, Peter

Year: 2023

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-023-02132-z

Paper From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pastoris.

Tir, Nora; Heistinger, Lina; Grünwald-Gruber, Clemens et al. · 2022 · Microbial cell factories · DOI

Full title: From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pastoris.

Authors: Tir, Nora; Heistinger, Lina; Grünwald-Gruber, Clemens; Jakob, Leo A; Dickgiesser, Stephan; Rasche, Nicolas; Mattanovich, Diethard

Year: 2022

Venue: Microbial cell factories

DOI: https://doi.org/10.1186/s12934-022-01882-6

Paper Heterologous production of the insecticidal pea seed albumin PA1 protein by Pichia pastoris and protein engineering to potentiate aphicidal activity via fusion to snowdrop lectin Galanthus nivalis agglutinin; GNA)

Jake S. De-Thier; Prashant S Pyati; Jack Bell et al. · 2023 · Microbial Cell Factories · DOI

Full title: Heterologous production of the insecticidal pea seed albumin PA1 protein by Pichia pastoris and protein engineering to potentiate aphicidal activity via fusion to snowdrop lectin Galanthus nivalis agglutinin; GNA)

Authors: Jake S. De-Thier; Prashant S Pyati; Jack Bell; Jennifer J Readshaw; Adrian P. Brown; E. Fitches

Year: 2023

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-023-02176-1

Paper Metabolic engineering of Pichia pastoris for myo-inositol production by dynamic regulation of central metabolism

Qi-Qi Zhang; Xiaolu Wang; Huiying Luo et al. · 2022 · Microbial Cell Factories · DOI

Full title: Metabolic engineering of Pichia pastoris for myo-inositol production by dynamic regulation of central metabolism

Authors: Qi-Qi Zhang; Xiaolu Wang; Huiying Luo; Ya-ru Wang; Yuan Wang; T. Tu; Xing Qin; Xiaoyun Su; Huo-qing Huang; B. Yao; Yingguo Bai; Jie Zhang

Year: 2022

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-022-01837-x

Paper Simultaneous enzyme production, Levan-type FOS synthesis and sugar by-products elimination using a recombinant Pichia pastoris strain expressing a levansucrase-endolevanase fusion enzyme

Ávila-Fernández, Ángela; Montiel, Silvia; Rodríguez-Alegría, Maria Elena et al. · 2023 · Microbial Cell Factories · DOI

Full title: Simultaneous enzyme production, Levan-type FOS synthesis and sugar by-products elimination using a recombinant Pichia pastoris strain expressing a levansucrase-endolevanase fusion enzyme

Authors: Ávila-Fernández, Ángela; Montiel, Silvia; Rodríguez-Alegría, Maria Elena; Caspeta, Luis; López-Munguı́a, Agustı́n

Year: 2023

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-022-02009-7

Paper Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near-zero growth rates

C. Rebnegger; Ben Coltman; Viktoria Kowarz et al. · 2024 · Microbial Cell Factories · DOI

Full title: Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near-zero growth rates

Authors: C. Rebnegger; Ben Coltman; Viktoria Kowarz; David A. Peña; Axel Mentler; Christina Troyer; Stephan Hann; Harald Schöny; G. Koellensperger; D. Mattanovich; Brigitte Gasser

Year: 2024

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-024-02314-3

Paper Challenges and advances in the heterologous expression of cellulolytic enzymes: a review

Lambertz, Camilla; Garvey, Megan; Klinger, Johannes et al. · 2014 · Biotechnology for Biofuels · DOI

Full title: Challenges and advances in the heterologous expression of cellulolytic enzymes: a review

Authors: Lambertz, Camilla; Garvey, Megan; Klinger, Johannes; Heesel, Dirk; Klose, Holger; Fischer, Rainer; Commandeur, Ulrich

Year: 2014

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-014-0135-5

Paper A supernumerary synthetic chromosome in Komagataella phaffii as a repository for extraneous genetic material

Dariusz Abramczyk; María del Carmen Sánchez Olmos; A. A. Ramírez Rojas et al. · 2023 · bioRxiv · DOI

Full title: A supernumerary synthetic chromosome in Komagataella phaffii as a repository for extraneous genetic material

Authors: Dariusz Abramczyk; María del Carmen Sánchez Olmos; A. A. Ramírez Rojas; Daniel Schindler; Daniel Robertson; Stephen McColm; A. Marston; Paul N. Barlow

Year: 2023

Venue: bioRxiv

DOI: https://doi.org/10.1186/s12934-023-02262-4

Paper Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability

Ma, Junyan; Li, Qian; Tan, Haidong et al. · 2019 · Journal of Biological Engineering · DOI

Full title: Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability

Authors: Ma, Junyan; Li, Qian; Tan, Haidong; Jiang, Hao; Li, Kuikui; Zhang, Lihua; Shi, Quan; Yin, Heng

Year: 2019

Venue: Journal of Biological Engineering

DOI: https://doi.org/10.1186/s13036-019-0215-y

Paper Next-generation stress-inducible Komagataella phaffii promoter variants.

Ebner, Katharina; Bernat-Camps, Núria; Scheipel, Simona et al. · 2025 · Microbial cell factories · DOI

Full title: Next-generation stress-inducible Komagataella phaffii promoter variants.

Authors: Ebner, Katharina; Bernat-Camps, Núria; Scheipel, Simona; Dörner, Corina; Valero, Francisco; Glieder, Anton; Garcia-Ortega, Xavier

Year: 2025

Venue: Microbial cell factories

DOI: https://doi.org/10.1186/s12934-025-02825-7

Paper Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris

Tachioka, Mikako; Sugimoto, Naohisa; Nakamura, Akihiko et al. · 2016 · Biotechnology for Biofuels · DOI

Full title: Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris

Authors: Tachioka, Mikako; Sugimoto, Naohisa; Nakamura, Akihiko; Sunagawa, Naoki; Ishida, Takuya; Uchiyama, Taku; Igarashi, Kiyohiko; Samejima, Masahiro

Year: 2016

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-016-0613-z

Paper Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

Florian Weiss; Guillermo Requena-Moreno; Carsten Pichler et al. · 2024 · Microbial Cell Factories · DOI

Full title: Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

Authors: Florian Weiss; Guillermo Requena-Moreno; Carsten Pichler; Francisco Valero; Anton Glieder; Xavier Garcia-Ortega

Year: 2024

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-024-02368-3

Paper Substrate specificity and regioselectivity of fungal AA9 lytic polysaccharide monooxygenases secreted by Podospora anserina

Bennati-Granier, Chloé; Garajová, Soňa; Champion, Charlotte et al. · 2015 · Biotechnology for Biofuels · DOI

Full title: Substrate specificity and regioselectivity of fungal AA9 lytic polysaccharide monooxygenases secreted by Podospora anserina

Authors: Bennati-Granier, Chloé; Garajová, Soňa; Champion, Charlotte; Grisel, Sacha; Haon, Mireille; Zhou, Simeng; Fanuel, Mathieu; Ropartz, David; Rogniaux, Hélène; Gimbert, Isabelle; Record, Éric; Berrin, Jean‐Guy

Year: 2015

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-015-0274-3

Paper Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae

Larue, Kane; Melgar, Mindy; Martin, Vincent J. J. · 2016 · Biotechnology for Biofuels · DOI

Full title: Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae

Authors: Larue, Kane; Melgar, Mindy; Martin, Vincent J. J.

Year: 2016

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-016-0470-9

Paper Expression and characterization of a novel 1,3-regioselective cold-adapted lipase from Rhizomucor endophyticus suitable for biodiesel synthesis

Yan, Qiaojuan; Duan, Xiaojie; Liu, Yu et al. · 2016 · Biotechnology for Biofuels · DOI

Full title: Expression and characterization of a novel 1,3-regioselective cold-adapted lipase from Rhizomucor endophyticus suitable for biodiesel synthesis

Authors: Yan, Qiaojuan; Duan, Xiaojie; Liu, Yu; Jiang, Zhengqiang; Yang, Shaoqing

Year: 2016

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-016-0501-6

Paper A constitutive expression system for glycosyl hydrolase family 7 cellobiohydrolases in Hypocrea jecorina

Linger, Jeffrey; Taylor, Larry E.; Baker, John O. et al. · 2015 · Biotechnology for Biofuels · DOI

Full title: A constitutive expression system for glycosyl hydrolase family 7 cellobiohydrolases in Hypocrea jecorina

Authors: Linger, Jeffrey; Taylor, Larry E.; Baker, John O.; Wall, Todd Vander; Hobdey, Sarah E.; Podkaminer, Kara; Himmel, Michael E.; Decker, Stephen R.

Year: 2015

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-015-0230-2

Paper Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessing

Wei, Hui; Wang, Wei; Alahuhta, Markus et al. · 2014 · Biotechnology for Biofuels · DOI

Full title: Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessing

Authors: Wei, Hui; Wang, Wei; Alahuhta, Markus; Wall, Todd Vander; Baker, John O.; Taylor, Larry E.; Decker, Stephen R.; Himmel, Michael E.; Zhang, Min

Year: 2014

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-014-0148-0

Paper Whole-genome metabolic model of Trichoderma reesei built by comparative reconstruction

Castillo, Sandra; Barth, Dorothee; Arvas, Mikko et al. · 2016 · Biotechnology for Biofuels · DOI

Full title: Whole-genome metabolic model of Trichoderma reesei built by comparative reconstruction

Authors: Castillo, Sandra; Barth, Dorothee; Arvas, Mikko; Pakula, Tiina; Pitkänen, Esa; Blomberg, Peter; Seppänen‐Laakso, Tuulikki; Nygrén, Heli; Sivasiddarthan, Dhinakaran; Penttilä, Merja; Oja, Merja

Year: 2016

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-016-0665-0

Paper High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production

Duan, Xiaojie; Liu, Yu; You, Xin et al. · 2017 · Biotechnology for Biofuels · DOI

Full title: High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production

Authors: Duan, Xiaojie; Liu, Yu; You, Xin; Jiang, Zhengqiang; Yang, Shaoxiang; Yang, Shaoqing

Year: 2017

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-017-0912-z

Paper The yeast Geotrichum candidum encodes functional lytic polysaccharide monooxygenases

Ladevèze, Simon; Haon, Mireille; Villares, Ana et al. · 2017 · Biotechnology for Biofuels · DOI

Full title: The yeast Geotrichum candidum encodes functional lytic polysaccharide monooxygenases

Authors: Ladevèze, Simon; Haon, Mireille; Villares, Ana; Cathala, Bernard; Grisel, Sacha; Herpoël‐Gimbert, Isabelle; Henrissat, Bernard; Berrin, Jean‐Guy

Year: 2017

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-017-0903-0

Paper Improvement of the thermostability and catalytic efficiency of a highly active β-glucanase from Talaromyces leycettanus JCM12802 by optimizing residual charge–charge interactions

Shuai You; T. Tu; Lujia Zhang et al. · 2016 · Biotechnology for Biofuels · DOI

Full title: Improvement of the thermostability and catalytic efficiency of a highly active β-glucanase from Talaromyces leycettanus JCM12802 by optimizing residual charge–charge interactions

Authors: Shuai You; T. Tu; Lujia Zhang; Y. Wang; Huo-qing Huang; Rui Ma; P. Shi; Yingguo Bai; Xiaoyun Su; Zhemin Lin; Huiying Luo; B. Yao

Year: 2016

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-016-0544-8

Paper A novel, highly efficient β-glucosidase with a cellulose-binding domain: characterization and properties of native and recombinant proteins

Méndez-Líter, Juan A.; Gil-Muñoz, Jesús; Nieto‐Domínguez, Manuel et al. · 2017 · Biotechnology for Biofuels · DOI

Full title: A novel, highly efficient β-glucosidase with a cellulose-binding domain: characterization and properties of native and recombinant proteins

Authors: Méndez-Líter, Juan A.; Gil-Muñoz, Jesús; Nieto‐Domínguez, Manuel; Barriuso, Jorge; Eugenio, Laura I. de; Martı́nez, Marı́a Jesús

Year: 2017

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-017-0946-2

Paper Engineering a highly active thermophilic β-glucosidase to enhance its pH stability and saccharification performance

Xia, Wei; Xu, Xinxin; Qian, Lichun et al. · 2016 · Biotechnology for Biofuels · DOI

Full title: Engineering a highly active thermophilic β-glucosidase to enhance its pH stability and saccharification performance

Authors: Xia, Wei; Xu, Xinxin; Qian, Lichun; Shi, Pengjun; Bai, Yingguo; Luo, Huiying; Ma, Rui; Yao, Bin

Year: 2016

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-016-0560-8

Paper Peroxisomes and peroxisomal transketolase and transaldolase enzymes are essential for xylose alcoholic fermentation by the methylotrophic thermotolerant yeast, Ogataea (Hansenula) polymorpha

Kurylenko, Olena; Ruchała, Justyna; Vasylyshyn, Roksolana et al. · 2018 · Biotechnology for Biofuels · DOI

Full title: Peroxisomes and peroxisomal transketolase and transaldolase enzymes are essential for xylose alcoholic fermentation by the methylotrophic thermotolerant yeast, Ogataea (Hansenula) polymorpha

Authors: Kurylenko, Olena; Ruchała, Justyna; Vasylyshyn, Roksolana; Stasyk, Oleh; Dmytruk, Olena; Dmytruk, Kostyantyn; Sibirny, Andriy А.

Year: 2018

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-018-1203-z

Paper The β-glucosidase secreted by Talaromyces amestolkiae under carbon starvation: a versatile catalyst for biofuel production from plant and algal biomass

Méndez-Líter, Juan A.; Eugenio, Laura I. de; Prieto, Alicia et al. · 2018 · Biotechnology for Biofuels · DOI

Full title: The β-glucosidase secreted by Talaromyces amestolkiae under carbon starvation: a versatile catalyst for biofuel production from plant and algal biomass

Authors: Méndez-Líter, Juan A.; Eugenio, Laura I. de; Prieto, Alicia; Martı́nez, Marı́a Jesús

Year: 2018

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-018-1125-9

Paper Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate

Siripong, Wiparat; Wolf, Philipp; Kusumoputri, Theodora Puspowangi et al. · 2018 · Biotechnology for Biofuels · DOI

Full title: Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate

Authors: Siripong, Wiparat; Wolf, Philipp; Kusumoputri, Theodora Puspowangi; Downes, Joe; Kocharin, Kanokarn; Tanapongpipat, Sutipa; Runguphan, Weerawat

Year: 2018

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-017-1003-x

Paper Real-time adsorption and action of expansin on cellulose

Duan, Yuhao; Ma, Yuanyuan; Zhao, Xudong et al. · 2018 · Biotechnology for Biofuels · DOI

Full title: Real-time adsorption and action of expansin on cellulose

Authors: Duan, Yuhao; Ma, Yuanyuan; Zhao, Xudong; Huang, Renliang; Su, Rongxin; Qi, Wei; He, Zhimin

Year: 2018

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-018-1318-2

Paper Chaetomella raphigera β-glucosidase D2-BGL has intriguing structural features and a high substrate affinity that renders it an efficient cellulase supplement for lignocellulosic biomass hydrolysis

Kao, Mu‐Rong; Kuo, Hsion-Wen; Lee, Cheng-Chung et al. · 2019 · Biotechnology for Biofuels · DOI

Full title: Chaetomella raphigera β-glucosidase D2-BGL has intriguing structural features and a high substrate affinity that renders it an efficient cellulase supplement for lignocellulosic biomass hydrolysis

Authors: Kao, Mu‐Rong; Kuo, Hsion-Wen; Lee, Cheng-Chung; Huang, Kuan-Ying A.; Huang, Ting-Yen; Li, Chenwei; Chen, C. Will; Wang, Andrew H.‐J.; Yu, Su‐May; Ho, Tuan‐Hua David

Year: 2019

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-019-1599-0

Paper A novel fungal GH30 xylanase with xylobiohydrolase auxiliary activity

Katsimpouras, Constantinos; Dedes, Grigorios; Τhomaidis, Νikolaos S. et al. · 2019 · Biotechnology for Biofuels · DOI

Full title: A novel fungal GH30 xylanase with xylobiohydrolase auxiliary activity

Authors: Katsimpouras, Constantinos; Dedes, Grigorios; Τhomaidis, Νikolaos S.; Topakas, Evangelos

Year: 2019

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-019-1455-2

Paper Novel molecular biological tools for the efficient expression of fungal lytic polysaccharide monooxygenases in Pichia pastoris

Rieder, Lukas; Ebner, Katharina; Glieder, Anton et al. · 2021 · Biotechnology for Biofuels · DOI

Full title: Novel molecular biological tools for the efficient expression of fungal lytic polysaccharide monooxygenases in Pichia pastoris

Authors: Rieder, Lukas; Ebner, Katharina; Glieder, Anton; Sørlie, Morten

Year: 2021

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-021-01971-5

Paper Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

Laura Navone; T. Vogl; P. Luangthongkam et al. · 2021 · Biotechnology for Biofuels · DOI

Full title: Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

Authors: Laura Navone; T. Vogl; P. Luangthongkam; J. Blinco; C. Luna-Flores; Xiaojing Chen; Juhani von Hellens; S. Mahler; R. Speight

Year: 2021

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-021-01936-8

Paper Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution

Kao, Mu‐Rong; Yu, Su‐May; Ho, Tuan-H ua David · 2021 · Biotechnology for Biofuels · DOI

Full title: Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution

Authors: Kao, Mu‐Rong; Yu, Su‐May; Ho, Tuan-H ua David

Year: 2021

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-021-01973-3

Paper Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris

Lin, Nai-Xin; He, Rui-Zhen; Xu, Yan et al. · 2021 · Biotechnology for Biofuels · DOI

Full title: Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris

Authors: Lin, Nai-Xin; He, Rui-Zhen; Xu, Yan; Yu, Xiao-Wei

Year: 2021

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-021-02013-w

Paper Improvement of thermostability and catalytic efficiency of glucoamylase from Talaromyces leycettanus JCM12802 via site-directed mutagenesis to enhance industrial saccharification applications

Tong, Lige; Zheng, Jie; Wang, Xiao et al. · 2021 · Biotechnology for Biofuels · DOI

Full title: Improvement of thermostability and catalytic efficiency of glucoamylase from Talaromyces leycettanus JCM12802 via site-directed mutagenesis to enhance industrial saccharification applications

Authors: Tong, Lige; Zheng, Jie; Wang, Xiao; Wang, Xiaolu; Huang, Huoqing; Yang, Haomeng; Tu, Tao; Wang, Yuan; Bai, Yingguo; Yao, Bin; Luo, Huiying; Qin, Xing

Year: 2021

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-021-02052-3

Paper Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris

Aw, Rochelle; McKay, Paul F.; Shattock, Robin J. et al. · 2017 · AMB Express · DOI

Full title: Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris

Authors: Aw, Rochelle; McKay, Paul F.; Shattock, Robin J.; Polizzi, Karen M.

Year: 2017

Venue: AMB Express

DOI: https://doi.org/10.1186/s13568-017-0372-7

Paper Second generation Pichia pastoris strain and bioprocess designs

Ergün, Burcu Gündüz; Laçın, Kübra; Çaloğlu, Buse et al. · 2022 · Biotechnology for Biofuels and Bioproducts · DOI

Full title: Second generation Pichia pastoris strain and bioprocess designs

Authors: Ergün, Burcu Gündüz; Laçın, Kübra; Çaloğlu, Buse; Bi̇nay, Barış

Year: 2022

Venue: Biotechnology for Biofuels and Bioproducts

DOI: https://doi.org/10.1186/s13068-022-02234-7

Paper Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris

Yuan, Huiling; Zhou, Ying; Lin, Yuping et al. · 2022 · Biotechnology for Biofuels and Bioproducts · DOI

Full title: Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris

Authors: Yuan, Huiling; Zhou, Ying; Lin, Yuping; Tu, Ran; Guo, Yufeng; Zhang, Yuanyuan; Wang, Qinhong

Year: 2022

Venue: Biotechnology for Biofuels and Bioproducts

DOI: https://doi.org/10.1186/s13068-022-02150-w

Paper Characterization and modulation of endoplasmic reticulum stress response target genes in Kluyveromyces marxianus to improve secretory expressions of heterologous proteins

Shi, Tianfang; Zhou, Jungang; Xue, Aijuan et al. · 2021 · Biotechnology for Biofuels · DOI

Full title: Characterization and modulation of endoplasmic reticulum stress response target genes in Kluyveromyces marxianus to improve secretory expressions of heterologous proteins

Authors: Shi, Tianfang; Zhou, Jungang; Xue, Aijuan; Lü, Hong; He, Yungang; Yu, Yao

Year: 2021

Venue: Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-021-02086-7

Paper Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design

Li, Xueqing; Wu, Qin; Hu, Die et al. · 2017 · AMB Express · DOI

Full title: Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design

Authors: Li, Xueqing; Wu, Qin; Hu, Die; Wang, Rui; Liu, Yan; Wu, Minchen; Li, Jianfang

Year: 2017

Venue: AMB Express

DOI: https://doi.org/10.1186/s13568-017-0399-9

Paper Characterization of recombinant laccase from Trametes versicolor synthesized by Arxula adeninivorans and its application in the degradation of pharmaceuticals

Litwińska, Katarzyna; Bischoff, Felix; Matthes, Falko et al. · 2019 · AMB Express · DOI

Full title: Characterization of recombinant laccase from Trametes versicolor synthesized by Arxula adeninivorans and its application in the degradation of pharmaceuticals

Authors: Litwińska, Katarzyna; Bischoff, Felix; Matthes, Falko; Bode, Rüdiger; Rutten, Twan; Kunze, Gotthard

Year: 2019

Venue: AMB Express

DOI: https://doi.org/10.1186/s13568-019-0832-3

Paper Deciphering cell wall sensors enabling the construction of robust P. pastoris for single-cell protein production

Gao, Le; Meng, Jiao; Dai, Wuling et al. · 2023 · Biotechnology for Biofuels and Bioproducts · DOI

Full title: Deciphering cell wall sensors enabling the construction of robust P. pastoris for single-cell protein production

Authors: Gao, Le; Meng, Jiao; Dai, Wuling; Zhang, Zhaokun; Dong, Haofan; Yuan, Qianqian; Zhang, Wuyuan; Liu, Shuguang; Wu, Xin

Year: 2023

Venue: Biotechnology for Biofuels and Bioproducts

DOI: https://doi.org/10.1186/s13068-023-02428-7

Paper Improvement of efficiency of brown coal biosolubilization by novel recombinant Fusarium oxysporum laccase

Kwiatos, Natalia; Jędrzejczak‐Krzepkowska, Marzena; Strzelecki, Bartosz et al. · 2018 · AMB Express · DOI

Full title: Improvement of efficiency of brown coal biosolubilization by novel recombinant Fusarium oxysporum laccase

Authors: Kwiatos, Natalia; Jędrzejczak‐Krzepkowska, Marzena; Strzelecki, Bartosz; Bielecki, Stanisław

Year: 2018

Venue: AMB Express

DOI: https://doi.org/10.1186/s13568-018-0669-1

Paper The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella phaffii

Zou, Chenwei; Lu, Lingfang; Wang, Shengyan et al. · 2022 · Biotechnology for Biofuels and Bioproducts · DOI

Full title: The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella phaffii

Authors: Zou, Chenwei; Lu, Lingfang; Wang, Shengyan; Zhang, Chenshan; Chen, Xuequn; Lin, Yao; Huang, Yide

Year: 2022

Venue: Biotechnology for Biofuels and Bioproducts

DOI: https://doi.org/10.1186/s13068-022-02243-6

Paper The first fungal laccase with an alkaline pH optimum obtained by directed evolution and its application in indigo dye decolorization

Yin, Qiang; Zhou, Gang; Peng, Can et al. · 2019 · AMB Express · DOI

Full title: The first fungal laccase with an alkaline pH optimum obtained by directed evolution and its application in indigo dye decolorization

Authors: Yin, Qiang; Zhou, Gang; Peng, Can; Zhang, Yinliang; Kües, Ursula; Liu, Juanjuan; Xiao, Yazhong; Fang, Zemin

Year: 2019

Venue: AMB Express

DOI: https://doi.org/10.1186/s13568-019-0878-2

Paper Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila

Bonzom, Cyrielle; Hüttner, Silvia; Mirgorodskaya, Ekaterina et al. · 2019 · AMB Express · DOI

Full title: Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila

Authors: Bonzom, Cyrielle; Hüttner, Silvia; Mirgorodskaya, Ekaterina; Chong, Sun‐Li; Uthoff, Stefan; Steinbüchel, Alexander; Verhaert, R.M.D.; Olsson, Lisbeth

Year: 2019

Venue: AMB Express

DOI: https://doi.org/10.1186/s13568-019-0852-z

Paper Improved methanol-derived lovastatin production through enhancement of the biosynthetic pathway and intracellular lovastatin efflux in methylotrophic yeast

Liu, Yiqi; Bai, Chenxiao; Xu, Qin et al. · 2018 · Bioresources and Bioprocessing · DOI

Full title: Improved methanol-derived lovastatin production through enhancement of the biosynthetic pathway and intracellular lovastatin efflux in methylotrophic yeast

Authors: Liu, Yiqi; Bai, Chenxiao; Xu, Qin; Yu, Jiahui; Zhou, Xiangshan; Zhang, Yuanxing; Cai, Menghao

Year: 2018

Venue: Bioresources and Bioprocessing

DOI: https://doi.org/10.1186/s40643-018-0202-z

Paper Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris

Vogl, Thomas; Fischer, Jasmin Elgin; Hyden, Patrick et al. · 2020 · AMB Express · DOI

Full title: Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris

Authors: Vogl, Thomas; Fischer, Jasmin Elgin; Hyden, Patrick; Wasmayer, Richard; Sturmberger, Lukas; Glieder, Anton

Year: 2020

Venue: AMB Express

DOI: https://doi.org/10.1186/s13568-020-00972-1

Paper Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA:ethanol O-acyltransferase Eht1 or Eeb1

Zhuang, Shiwen; Fu, Junshu; Powell, Chris et al. · 2015 · SpringerPlus · DOI

Full title: Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA:ethanol O-acyltransferase Eht1 or Eeb1

Authors: Zhuang, Shiwen; Fu, Junshu; Powell, Chris; Huang, Jinhai; Xia, Yihe; Yan, Ruixiang

Year: 2015

Venue: SpringerPlus

DOI: https://doi.org/10.1186/s40064-015-1195-0

Paper Transcriptional regulatory networks of methanol-independent protein expression in Pichia pastoris under the AOX1 promoter with trans-acting elements engineering

Lei Shi; Jinjia Wang; Xiaolong Wang et al. · 2020 · Bioresources and Bioprocessing · DOI

Full title: Transcriptional regulatory networks of methanol-independent protein expression in Pichia pastoris under the AOX1 promoter with trans-acting elements engineering

Authors: Lei Shi; Jinjia Wang; Xiaolong Wang; Yuanxing Zhang; Zhiwei Song; Menghao Cai; Xiangshan Zhou

Year: 2020

Venue: Bioresources and Bioprocessing

DOI: https://doi.org/10.1186/s40643-020-00306-w

Paper Characterization, expression and application of a zearalenone degrading enzyme from Neurospora crassa

Bi, Ke; Zhang, Wen; Xiao, Zhizhuang et al. · 2018 · AMB Express · DOI

Full title: Characterization, expression and application of a zearalenone degrading enzyme from Neurospora crassa

Authors: Bi, Ke; Zhang, Wen; Xiao, Zhizhuang; Zhang, Dawei

Year: 2018

Venue: AMB Express

DOI: https://doi.org/10.1186/s13568-018-0723-z

Paper Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris

Ma, Lingling; Liang, Xiaolin; Yu, Shiqin et al. · 2022 · Bioresources and Bioprocessing · DOI

Full title: Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris

Authors: Ma, Lingling; Liang, Xiaolin; Yu, Shiqin; Zhou, Jingwen

Year: 2022

Venue: Bioresources and Bioprocessing

DOI: https://doi.org/10.1186/s40643-022-00606-3

Paper High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris

Zhi-Xiang Xiang; Jin-Song Gong; Jin-Hao Shi et al. · 2022 · Bioresources and Bioprocessing · DOI

Full title: High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris

Authors: Zhi-Xiang Xiang; Jin-Song Gong; Jin-Hao Shi; Chun-Fang Liu; Heng Li; Chang Su; Min Jiang; Zhenghong Xu; Jin-song Shi

Year: 2022

Venue: Bioresources and Bioprocessing

DOI: https://doi.org/10.1186/s40643-022-00605-4

Paper Development of a Pichia pastoris cell factory for efficient production of germacrene A: a precursor of β-elemene

Cheng, Jintao; Zuo, Yimeng; Liu, Gaofei et al. · 2023 · Bioresources and Bioprocessing · DOI

Full title: Development of a Pichia pastoris cell factory for efficient production of germacrene A: a precursor of β-elemene

Authors: Cheng, Jintao; Zuo, Yimeng; Liu, Gaofei; Li, Dongfang; Gao, Jucan; Xiao, Feng; Huang, Lei; Xu, Zhinan; Lian, Jiazhang

Year: 2023

Venue: Bioresources and Bioprocessing

DOI: https://doi.org/10.1186/s40643-023-00657-0

Paper Human LYPD8 protein inhibits motility of flagellated bacteria

Hsu, Chiao-Ching; Okumura, Ryu; Takeda, Kiyoshi · 2017 · Inflammation and Regeneration · DOI

Full title: Human LYPD8 protein inhibits motility of flagellated bacteria

Authors: Hsu, Chiao-Ching; Okumura, Ryu; Takeda, Kiyoshi

Year: 2017

Venue: Inflammation and Regeneration

DOI: https://doi.org/10.1186/s41232-017-0056-3

Paper From induction to secretion: a complicated route for cellulase production in Trichoderma reesei

Su, Yan; Xu, Yan; Yu, Xiao‐Wei · 2021 · Bioresources and Bioprocessing · DOI

Full title: From induction to secretion: a complicated route for cellulase production in Trichoderma reesei

Authors: Su, Yan; Xu, Yan; Yu, Xiao‐Wei

Year: 2021

Venue: Bioresources and Bioprocessing

DOI: https://doi.org/10.1186/s40643-021-00461-8

Paper Stabilisation of the Fc Fragment of Human IgG1 by Engineered Intradomain Disulfide Bonds

Wozniak‐Knopp, Gordana; Stadlmann, Johannes; Rüker, Florian · 2012 · PLoS ONE · DOI

Full title: Stabilisation of the Fc Fragment of Human IgG1 by Engineered Intradomain Disulfide Bonds

Authors: Wozniak‐Knopp, Gordana; Stadlmann, Johannes; Rüker, Florian

Year: 2012

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0030083

Paper Secretory expression of thermostable alkaline protease from Bacillus stearothermophilus FI by using native signal peptide and α -factor secretion signal in Pichia pastoris

Latiffi, Amaliawati Ahmad; Salleh, Abu Bakar; Rahman, Raja Noor Zaliha Raja Abd et al. · 2013 · Genes & Genetic Systems · DOI

Full title: Secretory expression of thermostable alkaline protease from Bacillus stearothermophilus FI by using native signal peptide and α -factor secretion signal in Pichia pastoris

Authors: Latiffi, Amaliawati Ahmad; Salleh, Abu Bakar; Rahman, Raja Noor Zaliha Raja Abd; Oslan, Siti Nurbaya; Basri, Mahiran

Year: 2013

Venue: Genes & Genetic Systems

DOI: https://doi.org/10.1266/ggs.88.85

Paper A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris

Bai, Jiangping; Swartz, Douglas J.; Protasevich, Irina I. et al. · 2011 · PLoS ONE · DOI

Full title: A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris

Authors: Bai, Jiangping; Swartz, Douglas J.; Protasevich, Irina I.; Brouillette, Christie G.; Harrell, Patina M.; Hildebrandt, Ellen; Gasser, Brigitte; Mattanovich, Diethard; Ward, Andrew B.; Chang, Geoffrey; Urbatsch, Ina L.

Year: 2011

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0022577

Paper Native-State Stability Determines the Extent of Degradation Relative to Secretion of Protein Variants from Pichia pastoris

G. Whyteside; M. Alcocer; J. Kumita et al. · 2011 · PLoS ONE · DOI

Full title: Native-State Stability Determines the Extent of Degradation Relative to Secretion of Protein Variants from Pichia pastoris

Authors: G. Whyteside; M. Alcocer; J. Kumita; C. Dobson; M. Lazarou; R. Pleass; D. Archer

Year: 2011

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0022692

Paper Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity.

Love, Kerry Routenberg; Politano, Timothy J; Panagiotou, Vasiliki et al. · 2012 · PloS one · DOI

Full title: Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity.

Authors: Love, Kerry Routenberg; Politano, Timothy J; Panagiotou, Vasiliki; Jiang, Bo; Stadheim, Terrance A; Love, J Christopher

Year: 2012

Venue: PloS one

DOI: https://doi.org/10.1371/journal.pone.0037915

Paper Amino Acid Substitutions at the Major Insertion Loop of Candida albicans Sterol 14alpha-Demethylase Are Involved in Fluconazole Resistance

Alvarez-Rueda, Nidia; Fleury, Audrey; Morio, Florent et al. · 2011 · PLoS ONE · DOI

Full title: Amino Acid Substitutions at the Major Insertion Loop of Candida albicans Sterol 14alpha-Demethylase Are Involved in Fluconazole Resistance

Authors: Alvarez-Rueda, Nidia; Fleury, Audrey; Morio, Florent; Pagniez, Fabrice; Gastinel, Louis N.; Pape, Patrice Le

Year: 2011

Venue: PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0021239

Paper Efficient protein production by yeast requires global tuning of metabolism

Huang, Mingtao; Bao, Jichen; Hallström, Björn M. et al. · 2017 · Nature Communications · DOI

Full title: Efficient protein production by yeast requires global tuning of metabolism

Authors: Huang, Mingtao; Bao, Jichen; Hallström, Björn M.; Petranović, Dina; Nielsen, Jens

Year: 2017

Venue: Nature Communications

DOI: https://doi.org/10.1038/s41467-017-00999-2

Paper Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care

Pérez‐Piñera, Pablo; Han, Ningren; Cleto, Sara et al. · 2016 · Nature Communications · DOI

Full title: Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care

Authors: Pérez‐Piñera, Pablo; Han, Ningren; Cleto, Sara; Cao, Jicong; Purcell, Oliver; Shah, Kartik; Lee, Kevin; Ram, Rajeev J.; Lu, Timothy K.

Year: 2016

Venue: Nature Communications

DOI: https://doi.org/10.1038/ncomms12211

Paper Genome sequence of the recombinant protein production host Pichia pastoris

Schutter, Kristof De; Lin, Yao‐Cheng; Tiels, Petra et al. · 2009 · Nature Biotechnology · DOI

Full title: Genome sequence of the recombinant protein production host Pichia pastoris

Authors: Schutter, Kristof De; Lin, Yao‐Cheng; Tiels, Petra; Hecke, Annelies Van; Glinka, Sascha; Weber-Lehmann, Jacqueline; Rouzé, Pierre; Peer, Yves Van de; Callewaert, Nico

Year: 2009

Venue: Nature Biotechnology

DOI: https://doi.org/10.1038/nbt.1544

Paper Versatile and on-demand biologics co-production in yeast

Cao, Jicong; Pérez‐Piñera, Pablo; Lowenhaupt, Ky et al. · 2018 · Nature Communications · DOI

Full title: Versatile and on-demand biologics co-production in yeast

Authors: Cao, Jicong; Pérez‐Piñera, Pablo; Lowenhaupt, Ky; Wu, Ming-Ru; Purcell, Oliver; Fuente‐Núñez, César de la; Lu, Timothy K.

Year: 2018

Venue: Nature Communications

DOI: https://doi.org/10.1038/s41467-017-02587-w

Paper RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes

Abatemarco, Joseph; Sarhan, Maen F.; Wagner, James M. et al. · 2017 · Nature Communications · DOI

Full title: RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes

Authors: Abatemarco, Joseph; Sarhan, Maen F.; Wagner, James M.; Lin, Jyun‐Liang; Liu, Leqian; Hassouneh, Wafa; Yuan, Shuo‐Fu; Alper, Hal S.; Abate, Adam R.

Year: 2017

Venue: Nature Communications

DOI: https://doi.org/10.1038/s41467-017-00425-7

Paper Engineering consortia by polymeric microbial swarmbots

Wang, Lin; Zhang, Xi; Tang, Chenwang et al. · 2022 · Nature Communications · DOI

Full title: Engineering consortia by polymeric microbial swarmbots

Authors: Wang, Lin; Zhang, Xi; Tang, Chenwang; Li, Pengcheng; Zhu, Runtao; Sun, Jing; Zhang, Yunfeng; Cui, Hua; Ma, Jiajia; Song, Xinyu; Zhang, Weiwen; Gao, Xiang; Luo, Xiaozhou; You, Lingchong; Chen, Ye; Dai, Zhuojun

Year: 2022

Venue: Nature Communications

DOI: https://doi.org/10.1038/s41467-022-31467-1

Paper A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast

Naseri, Gita · 2023 · Nature Communications · DOI

Full title: A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast

Authors: Naseri, Gita

Year: 2023

Venue: Nature Communications

DOI: https://doi.org/10.1038/s41467-023-37627-1

Paper A Cas3-base editing tool for targetable in vivo mutagenesis

Zimmermann, Anna; Prieto-Vivas, Julian E.; Cautereels, Charlotte et al. · 2023 · Nature Communications · DOI

Full title: A Cas3-base editing tool for targetable in vivo mutagenesis

Authors: Zimmermann, Anna; Prieto-Vivas, Julian E.; Cautereels, Charlotte; Gorkovskiy, Anton; Steensels, Jan; Peer, Yves Van de; Verstrepen, Kevin J.

Year: 2023

Venue: Nature Communications

DOI: https://doi.org/10.1038/s41467-023-39087-z

Paper Improving recombinant protein production by yeast through genome-scale modeling using proteome constraints

Li, Feiran; Chen, Yu; Qi, Qi et al. · 2022 · Nature Communications · DOI

Full title: Improving recombinant protein production by yeast through genome-scale modeling using proteome constraints

Authors: Li, Feiran; Chen, Yu; Qi, Qi; Wang, Yanyan; Yuan, Le; Huang, Mingtao; Elsemman, Ibrahim E.; Feizi, Amir; Kerkhoven, Eduard J.; Nielsen, Jens

Year: 2022

Venue: Nature Communications

DOI: https://doi.org/10.1038/s41467-022-30689-7

Paper Engineered bidirectional promoters enable rapid multi-gene co-expression optimization

T. Vogl; Thomas Kickenweiz; Julia Pitzer et al. · 2018 · Nature Communications · DOI

Full title: Engineered bidirectional promoters enable rapid multi-gene co-expression optimization

Authors: T. Vogl; Thomas Kickenweiz; Julia Pitzer; Lukas Sturmberger; A. Weninger; Bradley W. Biggs; Eva-Maria Köhler; Armin Baumschlager; J. E. Fischer; P. Hyden; Marlies Wagner; Martina Baumann; N. Borth; M. Geier; P. K. Ajikumar; A. Glieder

Year: 2018

Venue: Nature Communications

DOI: https://doi.org/10.1038/s41467-018-05915-w

Paper A polycistronic system for multiplexed and precalibrated expression of multigene pathways in fungi

Yue, Qun; Meng, Jie; Qiu, Yue et al. · 2023 · Nature Communications · DOI

Full title: A polycistronic system for multiplexed and precalibrated expression of multigene pathways in fungi

Authors: Yue, Qun; Meng, Jie; Qiu, Yue; Yin, Miaomiao; Zhang, Liwen; Zhou, Weiping; An, Zhiqiang; Liu, Zihe; Yuan, Qipeng; Sun, Wentao; Li, Chun; Zhao, Huimin; Molnár, István; Xu, Yuquan; Shi, Shuobo

Year: 2023

Venue: Nature Communications

DOI: https://doi.org/10.1038/s41467-023-40027-0

Paper Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris

Cheng, Li; Lin, Ying; Zheng, Xueyun et al. · 2017 · Scientific Reports · DOI

Full title: Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris

Authors: Cheng, Li; Lin, Ying; Zheng, Xueyun; Yuan, Qingyan; Pang, Nuo; Liao, Xihao; Huang, Yuanyuan; Zhang, Xinying; Liang, Shuli

Year: 2017

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-017-11494-5

Paper Immobilized enzyme cascade for targeted glycosylation

Makrydaki, Elli; Donini, Roberto; Krueger, A. P. et al. · 2024 · Nature Chemical Biology · DOI

Full title: Immobilized enzyme cascade for targeted glycosylation

Authors: Makrydaki, Elli; Donini, Roberto; Krueger, A. P.; Royle, Kate E.; Moya‐Ramírez, Ignacio; Kuntz, D.A.; Rose, David R.; Haslam, Stuart M.; Polizzi, Karen M.; Kontoravdi, Cleo

Year: 2024

Venue: Nature Chemical Biology

DOI: https://doi.org/10.1038/s41589-023-01539-4

Paper Controlling AOX1 promoter strength in Pichia pastoris by manipulating poly (dA:dT) tracts

Jun Yang; Haiming Cai; Jie Liu et al. · 2018 · Scientific Reports · DOI

Full title: Controlling AOX1 promoter strength in Pichia pastoris by manipulating poly (dA:dT) tracts

Authors: Jun Yang; Haiming Cai; Jie Liu; Min Zeng; Jiawei Chen; Qingmei Cheng; Linghua Zhang

Year: 2018

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-018-19831-y

Paper An improved pathway for autonomous bioluminescence imaging in eukaryotes

Shakhova, Ekaterina S.; Karataeva, Tatiana A.; Markina, Nadezhda M. et al. · 2024 · Nature Methods · DOI

Full title: An improved pathway for autonomous bioluminescence imaging in eukaryotes

Authors: Shakhova, Ekaterina S.; Karataeva, Tatiana A.; Markina, Nadezhda M.; Mitiouchkina, Tatiana; Palkina, Kseniia A.; Perfilov, Maxim M.; Wood, Monika G.; Hoang, Trish T.; Hall, Mary P.; Fakhranurova, Liliia I.; Alekberova, Anna E.; Malyshevskaia, Alena K.; Gorbachev, D.A.; Bugaeva, Evgenia N.; Pletneva, Ludmila K.; Babenko, Vladislav V.; Boldyreva, Daria I.; Gorokhovatsky, Andrey Yu.; Balakireva, Anastasia V.; Gao, Feng; Choob, V. V.; Encell, Lance P.; Wood, Keith V.; Yampolsky, Ilia V.; Sarkisyan, Karen S.; Mishin, Alexander S.

Year: 2024

Venue: Nature Methods

DOI: https://doi.org/10.1038/s41592-023-02152-y

Paper High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin

Chen, Ximing; Li, Juan; Sun, Haili et al. · 2017 · Scientific Reports · DOI

Full title: High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin

Authors: Chen, Ximing; Li, Juan; Sun, Haili; Li, Shi-Weng; Chen, Tuo; Liu, Guangxiu; Dyson, Paul

Year: 2017

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-017-15149-3

Paper OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression

Claes, Katrien; Herpe, Dries Van; Vanluchene, Robin et al. · 2024 · Nature Microbiology · DOI

Full title: OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression

Authors: Claes, Katrien; Herpe, Dries Van; Vanluchene, Robin; Roels, Charlotte; Moer, Berre Van; Wyseure, Elise; Vandewalle, Kristof; Eeckhaut, Hannah; Yılmaz, Semiramis; Vanmarcke, Sandrine; Çıtak, Erhan; Fijałkowska, Daria; Grootaert, Hendrik; Lonigro, Chiara; Meuris, Leander; Michielsen, Gitte; Naessens, Justine; Schie, Loes van; Rycke, Riet De; Bruyne, Michiel De; Borghgraef, Peter; Callewaert, Nico

Year: 2024

Venue: Nature Microbiology

DOI: https://doi.org/10.1038/s41564-023-01574-w

Paper Efficient production and characterization of the novel and highly active antifungal protein AfpB from Penicillium digitatum

Garrigues, Sandra; Gandía, Mónica; Popa, Crina et al. · 2017 · Scientific Reports · DOI

Full title: Efficient production and characterization of the novel and highly active antifungal protein AfpB from Penicillium digitatum

Authors: Garrigues, Sandra; Gandía, Mónica; Popa, Crina; Borics, Attila; Marx, Florentine; Coca, María; Marcos, José F.; Manzanares, Paloma

Year: 2017

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-017-15277-w

Paper Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects

Yu, Xiao‐Wei; Sun, Weihong; Wang, Ying-Zheng et al. · 2017 · Scientific Reports · DOI

Full title: Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects

Authors: Yu, Xiao‐Wei; Sun, Weihong; Wang, Ying-Zheng; Xu, Yan

Year: 2017

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-017-16577-x

Paper Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B

Theron, Chrispian W.; Vandermies, Marie; Telek, Samuel et al. · 2020 · Scientific Reports · DOI

Full title: Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B

Authors: Theron, Chrispian W.; Vandermies, Marie; Telek, Samuel; Steels, Sébastien; Fickers, Patrick

Year: 2020

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-020-58683-3

Paper The effect of non-thermal atmospheric plasma on the production and activity of recombinant phytase enzyme

Farasat, Mahsa; Arjmand, Sareh; Siadat, Seyed Omid Ranaei et al. · 2018 · Scientific Reports · DOI

Full title: The effect of non-thermal atmospheric plasma on the production and activity of recombinant phytase enzyme

Authors: Farasat, Mahsa; Arjmand, Sareh; Siadat, Seyed Omid Ranaei; Sefidbakht, Yahya; Ghomi, Hamid

Year: 2018

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-018-34239-4

Paper Evolving stability and pH-dependent activity of the high redox potential Botrytis aclada laccase for enzymatic fuel cells

Scheiblbrandner, Stefan; Breslmayr, Erik; Csarman, Florian et al. · 2017 · Scientific Reports · DOI

Full title: Evolving stability and pH-dependent activity of the high redox potential Botrytis aclada laccase for enzymatic fuel cells

Authors: Scheiblbrandner, Stefan; Breslmayr, Erik; Csarman, Florian; Paukner, Regina; Führer, Johannes; Herzog, Peter L.; Shleev, Sergey; Osipov, Evgenii M.; Тихонова, Т.В.; Popov, Vladimir O.; Haltrich, Dietmar; Ludwig, Roland; Kittl, Roman

Year: 2017

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-017-13734-0

Paper Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells

Arbeitman, Claudia R.; Auge, Gabriela; Blaustein, Matı́as et al. · 2020 · Scientific Reports · DOI

Full title: Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells

Authors: Arbeitman, Claudia R.; Auge, Gabriela; Blaustein, Matı́as; Bredeston, Luis M.; Corapi, Enrique; Craig, Patricio O.; Cossio, Leandro A.; Daín, Liliana; D’Alessio, Cecilia; Elias, Fernanda; Fernández, Natalia; Gándola, Yamila B.; Gasulla, Javier; Gorojovsky, Natalia; Gudesblat, Gustavo E.; Herrera, María Georgina; Ibañez, Lorena I.; Idrovo, Tommy; Rando, Matías Iglesias; Kamenetzky, Laura; Nadra, Alejandro D.; Noseda, Diego G.; Paván, Carlos H.; Pavan, María Florencia; Pignataro, María F.; Román, Ernesto A.; Ruberto, Lucas; Rubinstein, Natalia; Santos, Javier; Velázquez, Francisco; Zelada, Alicia M.

Year: 2020

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-020-78711-6

Paper Improved thermostability of an acidic xylanase from Aspergillus sulphureus by combined disulphide bridge introduction and proline residue substitution

Yang, Wenhan; Yang, Yongzhi; Zhang, Lingdi et al. · 2017 · Scientific Reports · DOI

Full title: Improved thermostability of an acidic xylanase from Aspergillus sulphureus by combined disulphide bridge introduction and proline residue substitution

Authors: Yang, Wenhan; Yang, Yongzhi; Zhang, Lingdi; Xu, Hang; Guo, Xiaojing; Yang, Xu; Dong, Bing; Cao, Yunhe

Year: 2017

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-017-01758-5

Paper FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mammalian Reporter Cells

Yanakieva, Desislava; Elter, Adrian; Bratsch, Jens et al. · 2020 · Scientific Reports · DOI

Full title: FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mammalian Reporter Cells

Authors: Yanakieva, Desislava; Elter, Adrian; Bratsch, Jens; Friedrich, Karlheinz; Becker, Stefan; Kolmar, Harald

Year: 2020

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-020-66927-5

Paper Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clones in microscale

Kaushik, Neha; Lamminmäki, Urpo; Khanna, Navin et al. · 2020 · Scientific Reports · DOI

Full title: Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clones in microscale

Authors: Kaushik, Neha; Lamminmäki, Urpo; Khanna, Navin; Batra, Gaurav

Year: 2020

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-020-63995-5

Paper Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

Binyang Deng; Y. Yue; Jun Yang et al. · 2023 · Communications Biology · DOI

Full title: Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

Authors: Binyang Deng; Y. Yue; Jun Yang; Mingjun Yang; Qiong Xing; Hang Peng; Fei Wang; Ming Li; Lixin Ma; C. Zhai

Year: 2023

Venue: Communications Biology

DOI: https://doi.org/10.1038/s42003-023-04413-0

Paper High-yield novel leech hyaluronidase to expedite the preparation of specific hyaluronan oligomers

Jin, Peng; Kang, Zhen; Zhang, Na et al. · 2014 · Scientific Reports · DOI

Full title: High-yield novel leech hyaluronidase to expedite the preparation of specific hyaluronan oligomers

Authors: Jin, Peng; Kang, Zhen; Zhang, Na; Du, Guocheng; Chen, Jian

Year: 2014

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep04471

Paper Cold atmospheric plasma treatment enhances recombinant model protein production in yeast Pichia pastoris

Zeinab Kabarkouhi; S. Arjmand; Seyed Omid Ranaei Siadat et al. · 2023 · Scientific Reports · DOI

Full title: Cold atmospheric plasma treatment enhances recombinant model protein production in yeast Pichia pastoris

Authors: Zeinab Kabarkouhi; S. Arjmand; Seyed Omid Ranaei Siadat; B. Shokri

Year: 2023

Venue: Scientific Reports

DOI: https://doi.org/10.1038/s41598-023-34078-y

Paper Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris

Yu, Ping; Yan, Yuan; Gu, Qing et al. · 2013 · Scientific Reports · DOI

Full title: Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris

Authors: Yu, Ping; Yan, Yuan; Gu, Qing; Wang, Xiangyang

Year: 2013

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep03043

Paper Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement

Liu, Wan-Cang; Gong, Ting; Wang, Qinghua et al. · 2016 · Scientific Reports · DOI

Full title: Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement

Authors: Liu, Wan-Cang; Gong, Ting; Wang, Qinghua; Liang, Xiao; Chen, Jingjing; Zhu, Ping

Year: 2016

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep18439

Paper Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts

Schütz, Marco; Schöppe, Jendrik; Sedlák, Erik et al. · 2016 · Scientific Reports · DOI

Full title: Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts

Authors: Schütz, Marco; Schöppe, Jendrik; Sedlák, Erik; Hillenbrand, Matthias; Nagy‐Davidescu, Gabriela; Ehrenmann, Janosch; Klenk, Christoph; Egloff, Pascal; Kummer, Lutz; Plückthun, Andreas

Year: 2016

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep21508

Paper An Efficient Genome-Wide Fusion Partner Screening System for Secretion of Recombinant Proteins in Yeast

Bae, Jung-Hoon; Sung, Bong Hyun; Kim, Hyun‐Jin et al. · 2015 · Scientific Reports · DOI

Full title: An Efficient Genome-Wide Fusion Partner Screening System for Secretion of Recombinant Proteins in Yeast

Authors: Bae, Jung-Hoon; Sung, Bong Hyun; Kim, Hyun‐Jin; Park, Soon-Ho; Lim, Kwang-Mook; Kim, Mi Jin; Lee, Cho-Ryong; Sohn, Jung‐Hoon

Year: 2015

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep12229

Paper Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris

Jinjia Wang; Xiaolong Wang; Lei Shi et al. · 2017 · Scientific Reports · DOI

Full title: Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris

Authors: Jinjia Wang; Xiaolong Wang; Lei Shi; Fei Qi; Ping Zhang; Yuanxing Zhang; Xiangshan Zhou; Zhiwei Song; Menghao Cai

Year: 2017

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep41850

Paper Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris

Krainer, Florian; Gmeiner, Christoph; Neutsch, Lukas et al. · 2013 · Scientific Reports · DOI

Full title: Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris

Authors: Krainer, Florian; Gmeiner, Christoph; Neutsch, Lukas; Windwarder, Markus; Pletzenauer, Robert; Herwig, Christoph; Altmann, Friedrich; Glieder, Anton; Spadiut, Oliver

Year: 2013

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep03279

Paper A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix

Liang, Hui; Li, Xiaoran; Wang, Bin et al. · 2016 · Scientific Reports · DOI

Full title: A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix

Authors: Liang, Hui; Li, Xiaoran; Wang, Bin; Chen, Bing; Zhao, Yannan; Sun, Jie; Zhuang, Yan; Shi, Jiajia; Shen, He; Zhang, Zhijun; Dai, Jianwu

Year: 2016

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep18205

Paper Functional diversity and properties of multiple xylanases from Penicillium oxalicum GZ-2

Liao, Hanpeng; Zheng, Haiping; Li, Shuixian et al. · 2015 · Scientific Reports · DOI

Full title: Functional diversity and properties of multiple xylanases from Penicillium oxalicum GZ-2

Authors: Liao, Hanpeng; Zheng, Haiping; Li, Shuixian; Wei, Zhong; Mei, Xinlan; Ma, Hongyu; Shen, Qirong; Xu, Yangchun

Year: 2015

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep12631

Paper Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris

Cámara, Elena; Landes, Nils; Albiol, Joan et al. · 2017 · Scientific Reports · DOI

Full title: Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris

Authors: Cámara, Elena; Landes, Nils; Albiol, Joan; Gasser, Brigitte; Mattanovich, Diethard; Ferrer, Pau

Year: 2017

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep44302

Paper Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastoris

Rodríguez‐López, Alexander; Alméciga-Díaz, Carlos Javier; Sánchez, Jhonnathan et al. · 2016 · Scientific Reports · DOI

Full title: Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastoris

Authors: Rodríguez‐López, Alexander; Alméciga-Díaz, Carlos Javier; Sánchez, Jhonnathan; Moreno, Jefferson; Beltrán, Laura; Díaz, Dennis; Pardo, Andrea; Ramírez, Aura M.; Espejo, Ángela J.; Pimentel, Luisa Natalia; Barrera, Luis Alejandro

Year: 2016

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep29329

Paper Enhanced and homogeneous oxygen availability during incubation of microfluidic droplets

Mahler, Lisa; Tovar, Miguel; Weber, Thomas et al. · 2015 · RSC Advances · DOI

Full title: Enhanced and homogeneous oxygen availability during incubation of microfluidic droplets

Authors: Mahler, Lisa; Tovar, Miguel; Weber, Thomas; Brandes, Susanne; Rudolph, Martin; Ehgartner, Josef; Mayr, Torsten; Figge, Marc Thilo; Roth, Martin M.; Zang, Emerson

Year: 2015

Venue: RSC Advances

DOI: https://doi.org/10.1039/c5ra20118g

Paper Multi-level glyco-engineering techniques to generate IgG with defined Fc-glycans

Dekkers, Gillian; Plomp, Rosina; Koeleman, Carolien A. M. et al. · 2016 · Scientific Reports · DOI

Full title: Multi-level glyco-engineering techniques to generate IgG with defined Fc-glycans

Authors: Dekkers, Gillian; Plomp, Rosina; Koeleman, Carolien A. M.; Visser, Remco; Horsten, Hans Henning von; Sandig, Volker; Rispens, Theo; Wuhrer, Manfred; Vidarsson, Gestur

Year: 2016

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep36964

Paper Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy

Yunfeng Zhang; Zhenmin Ling; G. Du et al. · 2016 · Scientific Reports · DOI

Full title: Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy

Authors: Yunfeng Zhang; Zhenmin Ling; G. Du; Jian Chen; Zhen Kang

Year: 2016

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep23158

Paper High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation

Lu, Yihong; Fang, Cheng; Wang, Qinhong et al. · 2016 · Scientific Reports · DOI

Full title: High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation

Authors: Lu, Yihong; Fang, Cheng; Wang, Qinhong; Zhou, Yuling; Zhang, Guimin; Ma, Yanhe

Year: 2016

Venue: Scientific Reports

DOI: https://doi.org/10.1038/srep37869

Paper A Review of Xylanase Production by the Fermentation of Xylan: Classification, Characterization and Applications

L., F.; Andrade, Cristiane Conte Paim de; Sant, Maria Helena A. · 2013 · InTech eBooks · DOI

Full title: A Review of Xylanase Production by the Fermentation of Xylan: Classification, Characterization and Applications

Authors: L., F.; Andrade, Cristiane Conte Paim de; Sant, Maria Helena A.

Year: 2013

Venue: InTech eBooks

DOI: https://doi.org/10.5772/53544

Paper Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications

Parashar, Deepak; Satyanarayana, T. · 2017 · Frontiers in Microbiology · DOI

Full title: Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications

Authors: Parashar, Deepak; Satyanarayana, T.

Year: 2017

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2017.00493

Paper A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris

Li, Ding; Zhang, Bo; Li, Shu‐Ting et al. · 2017 · Frontiers in Microbiology · DOI

Full title: A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris

Authors: Li, Ding; Zhang, Bo; Li, Shu‐Ting; Zhou, Jie; Cao, Hui; Huang, Yan; Cui, Zhongli

Year: 2017

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2017.01698

Paper Pichia pastoris-Expressed Bivalent Virus-Like Particulate Vaccine Induces Domain III-Focused Bivalent Neutralizing Antibodies without Antibody-Dependent Enhancement in Vivo

Shukla, Rahul; Rajpoot, Ravi Kant; Arora, Upasana et al. · 2018 · Frontiers in Microbiology · DOI

Full title: Pichia pastoris-Expressed Bivalent Virus-Like Particulate Vaccine Induces Domain III-Focused Bivalent Neutralizing Antibodies without Antibody-Dependent Enhancement in Vivo

Authors: Shukla, Rahul; Rajpoot, Ravi Kant; Arora, Upasana; Poddar, Ankur; Swaminathan, S.; Khanna, Navin

Year: 2018

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2017.02644

Paper Enzymatic Degradation of Aromatic and Aliphatic Polyesters by P. pastoris Expressed Cutinase 1 from Thermobifida cellulosilytica

Gamerith, Caroline; Vastano, Marco; Ghorbanpour, Sahar et al. · 2017 · Frontiers in Microbiology · DOI

Full title: Enzymatic Degradation of Aromatic and Aliphatic Polyesters by P. pastoris Expressed Cutinase 1 from Thermobifida cellulosilytica

Authors: Gamerith, Caroline; Vastano, Marco; Ghorbanpour, Sahar; Zitzenbacher, Sabine; Ribitsch, Doris; Zumstein, Michael; Sander, Michael; Acero, Enrique Herrero; Pellis, Alessandro; Guebitz, Georg M.

Year: 2017

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2017.00938

Paper Systematic Optimization of Protein Secretory Pathways in Saccharomyces cerevisiae to Increase Expression of Hepatitis B Small Antigen

Sheng, Jiayuan; Flick, Hunter; Feng, Xueyang · 2017 · Frontiers in Microbiology · DOI

Full title: Systematic Optimization of Protein Secretory Pathways in Saccharomyces cerevisiae to Increase Expression of Hepatitis B Small Antigen

Authors: Sheng, Jiayuan; Flick, Hunter; Feng, Xueyang

Year: 2017

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2017.00875

Paper Pichia pastoris-expressed dengue 3 envelope-based virus-like particles elicit predominantly domain III-focused high titer neutralizing antibodies

Tripathi, Lav; Mani, Shailendra; Raut, Rajendra et al. · 2015 · Frontiers in Microbiology · DOI

Full title: Pichia pastoris-expressed dengue 3 envelope-based virus-like particles elicit predominantly domain III-focused high titer neutralizing antibodies

Authors: Tripathi, Lav; Mani, Shailendra; Raut, Rajendra; Poddar, Ankur; Tyagi, Poornima; Arora, Upasana; Silva, Aravinda de; Swaminathan, S.; Khanna, Navin

Year: 2015

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2015.01005

Paper Four Inducible Promoters for Controlled Gene Expression in the Oleaginous Yeast Rhodotorula toruloides

Johns, Alexander; Love, John; Aves, Stephen J. · 2016 · Frontiers in Microbiology · DOI

Full title: Four Inducible Promoters for Controlled Gene Expression in the Oleaginous Yeast Rhodotorula toruloides

Authors: Johns, Alexander; Love, John; Aves, Stephen J.

Year: 2016

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2016.01666

Paper Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia pastoris

Liu, Wan-Cang; Xiang, Haibo; Zhang, Tao et al. · 2020 · Frontiers in Microbiology · DOI

Full title: Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia pastoris

Authors: Liu, Wan-Cang; Xiang, Haibo; Zhang, Tao; Pang, Xu; Su, Jing; Liu, Hongyu; Ma, Baiping; Yu, Liyan

Year: 2020

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2020.01988

Paper Single-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes With Pichia pastoris

Hana Raschmanová; Iwo Zamora; Martina Borčinová et al. · 2019 · Frontiers in Microbiology · DOI

Full title: Single-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes With Pichia pastoris

Authors: Hana Raschmanová; Iwo Zamora; Martina Borčinová; Patrick Meier; A. Weninger; Dominik Mächler; A. Glieder; K. Melzoch; Z. Knejzlík; K. Kovar

Year: 2019

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2019.00335

Paper Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide in Pichia pastoris

Popa, Crina; Shi, Xiaoqing; Ruiz, Tarik et al. · 2019 · Frontiers in Microbiology · DOI

Full title: Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide in Pichia pastoris

Authors: Popa, Crina; Shi, Xiaoqing; Ruiz, Tarik; Ferrer, Pau; Coca, María

Year: 2019

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2019.01472

Paper Komagataella phaffii as Emerging Model Organism in Fundamental Research.

Bernauer, Lukas; Radkohl, Astrid; Lehmayer, Leonie Gabriela Katharina et al. · 2020 · Frontiers in microbiology · DOI

Full title: Komagataella phaffii as Emerging Model Organism in Fundamental Research.

Authors: Bernauer, Lukas; Radkohl, Astrid; Lehmayer, Leonie Gabriela Katharina; Emmerstorfer-Augustin, Anita

Year: 2020

Venue: Frontiers in microbiology

DOI: https://doi.org/10.3389/fmicb.2020.607028

Paper Bioproduction of the Recombinant Sweet Protein Thaumatin: Current State of the Art and Perspectives

Joseph, Jewel Ann; Akkermans, Simen; Nimmegeers, Philippe et al. · 2019 · Frontiers in Microbiology · DOI

Full title: Bioproduction of the Recombinant Sweet Protein Thaumatin: Current State of the Art and Perspectives

Authors: Joseph, Jewel Ann; Akkermans, Simen; Nimmegeers, Philippe; Impe, Jan Van

Year: 2019

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2019.00695

Paper Insight to Improve α-L-Arabinofuranosidase Productivity in Pichia pastoris and Its Application on Corn Stover Degradation

Zheng, Fengzhen; Liu, Junquan; Basit, Abdul et al. · 2018 · Frontiers in Microbiology · DOI

Full title: Insight to Improve α-L-Arabinofuranosidase Productivity in Pichia pastoris and Its Application on Corn Stover Degradation

Authors: Zheng, Fengzhen; Liu, Junquan; Basit, Abdul; Miao, Ting; Jiang, Wei

Year: 2018

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2018.03016

Paper Sophisticated Cloning, Fermentation, and Purification Technologies for an Enhanced Therapeutic Protein Production: A Review

Gupta, Sanjeev Kumar; Shukla, Pratyoosh · 2017 · Frontiers in Pharmacology · DOI

Full title: Sophisticated Cloning, Fermentation, and Purification Technologies for an Enhanced Therapeutic Protein Production: A Review

Authors: Gupta, Sanjeev Kumar; Shukla, Pratyoosh

Year: 2017

Venue: Frontiers in Pharmacology

DOI: https://doi.org/10.3389/fphar.2017.00419

Paper Current advances of Pichia pastoris as cell factories for production of recombinant proteins

Pan, Yingjie; Jiao, Yang; Wu, Jianping et al. · 2022 · Frontiers in Microbiology · DOI

Full title: Current advances of Pichia pastoris as cell factories for production of recombinant proteins

Authors: Pan, Yingjie; Jiao, Yang; Wu, Jianping; Yang, Lirong; Fang, Hao

Year: 2022

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2022.1059777

Paper Molecular Modification of Kex2 P1’ Site Enhances Expression and Druggability of Fungal Defensin

Yanjie Jin; Na Yang; D. Teng et al. · 2023 · Antibiotics · DOI

Full title: Molecular Modification of Kex2 P1’ Site Enhances Expression and Druggability of Fungal Defensin

Authors: Yanjie Jin; Na Yang; D. Teng; Ya Hao; R. Mao; Jianhua Wang

Year: 2023

Venue: Antibiotics

DOI: https://doi.org/10.3390/antibiotics12040786

Paper Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering Approaches for Cellulose Saccharification

Dadwal, Anica; Sharma, Shilpa; Satyanarayana, T. · 2020 · Frontiers in Microbiology · DOI

Full title: Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering Approaches for Cellulose Saccharification

Authors: Dadwal, Anica; Sharma, Shilpa; Satyanarayana, T.

Year: 2020

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2020.01387

Paper Strategies for Optimizing the Production of Proteins and Peptides with Multiple Disulfide Bonds

Ma, Yunqi; Lee, Chang-Joo; Park, Jang-Su · 2020 · Antibiotics · DOI

Full title: Strategies for Optimizing the Production of Proteins and Peptides with Multiple Disulfide Bonds

Authors: Ma, Yunqi; Lee, Chang-Joo; Park, Jang-Su

Year: 2020

Venue: Antibiotics

DOI: https://doi.org/10.3390/antibiotics9090541

Paper Comparative transcriptomic analysis-based identification of the regulation of foreign proteins with different stabilities expressed in Pichia pastoris.

Niu, Tingting; Cui, Yi; Shan, Xu et al. · 2022 · Frontiers in microbiology · DOI

Full title: Comparative transcriptomic analysis-based identification of the regulation of foreign proteins with different stabilities expressed in Pichia pastoris.

Authors: Niu, Tingting; Cui, Yi; Shan, Xu; Qin, Shuzhen; Zhou, Xuejie; Wang, Rui; Chang, Alan; Ma, Nan; Jing, Jingjing; He, Jianwei

Year: 2022

Venue: Frontiers in microbiology

DOI: https://doi.org/10.3389/fmicb.2022.1074398

Paper A Valuable Product of Microbial Cell Factories: Microbial Lipase

Yao, Wentao; Liu, Kaiquan; Liu, Hongling et al. · 2021 · Frontiers in Microbiology · DOI

Full title: A Valuable Product of Microbial Cell Factories: Microbial Lipase

Authors: Yao, Wentao; Liu, Kaiquan; Liu, Hongling; Jiang, Yi; Wang, Ruiming; Wang, Wei; Wang, Tengfei

Year: 2021

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2021.743377

Paper Enhanced Recombinant Protein Production Under Special Environmental Stress

Chen, Xinyi; Li, Chun; Liu, Hu · 2021 · Frontiers in Microbiology · DOI

Full title: Enhanced Recombinant Protein Production Under Special Environmental Stress

Authors: Chen, Xinyi; Li, Chun; Liu, Hu

Year: 2021

Venue: Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2021.630814

Paper Green Production and Biotechnological Applications of Cell Wall Lytic Enzymes

Benedetti, Manuel; Locci, Federica; Gramegna, Giovanna et al. · 2019 · Applied Sciences · DOI

Full title: Green Production and Biotechnological Applications of Cell Wall Lytic Enzymes

Authors: Benedetti, Manuel; Locci, Federica; Gramegna, Giovanna; Sestili, Francesco; Savatin, Daniel V.

Year: 2019

Venue: Applied Sciences

DOI: https://doi.org/10.3390/app9235012

Paper Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii

Barone, Giovanni Davide; Emmerstorfer‐Augustin, Anita; Biundo, Antonino et al. · 2023 · Biomolecules · DOI

Full title: Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii

Authors: Barone, Giovanni Davide; Emmerstorfer‐Augustin, Anita; Biundo, Antonino; Pisano, Isabella; Coccetti, Paola; Mapelli, Valeria; Camattari, Andrea

Year: 2023

Venue: Biomolecules

DOI: https://doi.org/10.3390/biom13030441

Paper Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris.

Maity, Nitu; Mishra, Saroj · 2019 · Biomolecules · DOI

Full title: Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris.

Authors: Maity, Nitu; Mishra, Saroj

Year: 2019

Venue: Biomolecules

DOI: https://doi.org/10.3390/biom9100568

Paper Arming Technology in Yeast—Novel Strategy for Whole-cell Biocatalyst and Protein Engineering

Kuroda, Kouichi; Ueda, Mitsuyoshi · 2013 · Biomolecules · DOI

Full title: Arming Technology in Yeast—Novel Strategy for Whole-cell Biocatalyst and Protein Engineering

Authors: Kuroda, Kouichi; Ueda, Mitsuyoshi

Year: 2013

Venue: Biomolecules

DOI: https://doi.org/10.3390/biom3030632

Paper The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments

Pouzet, Sylvain; Banderas, Alvaro; Bec, Matthias Le et al. · 2020 · Bioengineering · DOI

Full title: The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments

Authors: Pouzet, Sylvain; Banderas, Alvaro; Bec, Matthias Le; Lautier, Thomas; Truan, Gilles; Hersen, Pascal

Year: 2020

Venue: Bioengineering

DOI: https://doi.org/10.3390/bioengineering7040151

Paper Pichia pastoris (Komagataella phaffii) as a Cost-Effective Tool for Vaccine Production for Low- and Middle-Income Countries (LMICs)

Magalhães, Salomé de Sá; Keshavarz‐Moore, Eli · 2021 · Bioengineering · DOI

Full title: Pichia pastoris (Komagataella phaffii) as a Cost-Effective Tool for Vaccine Production for Low- and Middle-Income Countries (LMICs)

Authors: Magalhães, Salomé de Sá; Keshavarz‐Moore, Eli

Year: 2021

Venue: Bioengineering

DOI: https://doi.org/10.3390/bioengineering8090119

Paper Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi

Aaron S. Birchfield; C. McIntosh · 2024 · BioTech · DOI

Full title: Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi

Authors: Aaron S. Birchfield; C. McIntosh

Year: 2024

Venue: BioTech

DOI: https://doi.org/10.3390/biotech13010004

Paper A Universal Strategy for the Efficient Expression of Nanobodies in Pichia pastoris

Yiheng Zheng; Bingkun Li; Shida Zhao et al. · 2024 · Fermentation · DOI

Full title: A Universal Strategy for the Efficient Expression of Nanobodies in Pichia pastoris

Authors: Yiheng Zheng; Bingkun Li; Shida Zhao; Jiawei Liu; Ding Li

Year: 2024

Venue: Fermentation

DOI: https://doi.org/10.3390/fermentation10010037

Paper Genetically Modified Micro-Organisms for Industrial Food Enzyme Production: An Overview

Deckers, Marie; Deforce, Dieter; Fraiture, Marie‐Alice et al. · 2020 · Foods · DOI

Full title: Genetically Modified Micro-Organisms for Industrial Food Enzyme Production: An Overview

Authors: Deckers, Marie; Deforce, Dieter; Fraiture, Marie‐Alice; Roosens, Nancy H. C.

Year: 2020

Venue: Foods

DOI: https://doi.org/10.3390/foods9030326

Paper Discovery and Heterologous Expression of Unspecific Peroxygenases

Ebner, Katharina; Pfeifenberger, Lukas Johannes; Rinnofner, Claudia et al. · 2023 · Catalysts · DOI

Full title: Discovery and Heterologous Expression of Unspecific Peroxygenases

Authors: Ebner, Katharina; Pfeifenberger, Lukas Johannes; Rinnofner, Claudia; Schusterbauer, Veronika; Glieder, Anton; Winkler, Margit

Year: 2023

Venue: Catalysts

DOI: https://doi.org/10.3390/catal13010206

Paper High-Level Secretory Production of Recombinant E2-Spy Antigen Protein via Combined Strategy in Pichia pastoris

Bingkun Li; Yiheng Zheng; Shida Zhao et al. · 2024 · Fermentation · DOI

Full title: High-Level Secretory Production of Recombinant E2-Spy Antigen Protein via Combined Strategy in Pichia pastoris

Authors: Bingkun Li; Yiheng Zheng; Shida Zhao; Yaohan Zhang; Ding Li

Year: 2024

Venue: Fermentation

DOI: https://doi.org/10.3390/fermentation10020099

Paper A Middle-Aged Enzyme Still in Its Prime: Recent Advances in the Field of Cutinases

Nikolaivits, Efstratios; Kanelli, Maria; Dimarogona, Maria et al. · 2018 · Catalysts · DOI

Full title: A Middle-Aged Enzyme Still in Its Prime: Recent Advances in the Field of Cutinases

Authors: Nikolaivits, Efstratios; Kanelli, Maria; Dimarogona, Maria; Topakas, Evangelos

Year: 2018

Venue: Catalysts

DOI: https://doi.org/10.3390/catal8120612

Paper Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3

Li, Tao; Ma, Jieying; Xu, Zehua et al. · 2019 · Genes · DOI

Full title: Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3

Authors: Li, Tao; Ma, Jieying; Xu, Zehua; Wang, Shuang; Wang, Nan; Shao, Shulin; Yang, Wei; Huang, Lin; Liu, Yihan

Year: 2019

Venue: Genes

DOI: https://doi.org/10.3390/genes10080606

Paper Combining Protein and Strain Engineering for the Production of Glyco-Engineered Horseradish Peroxidase C1A in Pichia pastoris

Capone, Simona; Ćorajević, Lejla; Bonifert, Günther et al. · 2015 · International Journal of Molecular Sciences · DOI

Full title: Combining Protein and Strain Engineering for the Production of Glyco-Engineered Horseradish Peroxidase C1A in Pichia pastoris

Authors: Capone, Simona; Ćorajević, Lejla; Bonifert, Günther; Murth, Patrick; Maresch, Daniel; Altmann, Friedrich; Herwig, Christoph; Spadiut, Oliver

Year: 2015

Venue: International Journal of Molecular Sciences

DOI: https://doi.org/10.3390/ijms161023127

Paper Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris

Wu, Jyh-Ming; Wang, Shin-Yao; Fu, Wei-Chang · 2012 · International Journal of Molecular Sciences · DOI

Full title: Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris

Authors: Wu, Jyh-Ming; Wang, Shin-Yao; Fu, Wei-Chang

Year: 2012

Venue: International Journal of Molecular Sciences

DOI: https://doi.org/10.3390/ijms131013212

Paper Enhanced Thermostability of Glucose Oxidase through Computer-Aided Molecular Design

Ning, Xiaoyan; Zhang, Yanli; Yuan, Tiantian et al. · 2018 · International Journal of Molecular Sciences · DOI

Full title: Enhanced Thermostability of Glucose Oxidase through Computer-Aided Molecular Design

Authors: Ning, Xiaoyan; Zhang, Yanli; Yuan, Tiantian; Li, Qingbin; Tian, Jian; Guan, Weishi; Liu, Bo; Zhang, Wei; Xu, Xinxin; Zhang, Yuhong; Zhang, Yuhong; Zhang, Yuhong

Year: 2018

Venue: International Journal of Molecular Sciences

DOI: https://doi.org/10.3390/ijms19020425

Paper Efficient Heterologous Production of Rhizopus oryzae Lipase via Optimization of Multiple Expression-Related Helper Proteins.

Jiao, Liangcheng; Zhou, Qinghua; Su, Zhixin et al. · 2018 · International journal of molecular sciences · DOI

Full title: Efficient Heterologous Production of Rhizopus oryzae Lipase via Optimization of Multiple Expression-Related Helper Proteins.

Authors: Jiao, Liangcheng; Zhou, Qinghua; Su, Zhixin; Yan, Yunjun

Year: 2018

Venue: International journal of molecular sciences

DOI: https://doi.org/10.3390/ijms19113372

Paper Production of a Recombinant Non-Hydroxylated Gelatin Mimetic in Pichia pastoris for Biomedical Applications

Gellermann, Pia; Schneider-Barthold, Caroline; Bolten, Svenja Nicolin et al. · 2019 · Journal of Functional Biomaterials · DOI

Full title: Production of a Recombinant Non-Hydroxylated Gelatin Mimetic in Pichia pastoris for Biomedical Applications

Authors: Gellermann, Pia; Schneider-Barthold, Caroline; Bolten, Svenja Nicolin; Overfelt, Ethan; Scheper, Thomas; Pepelanova, Iliyana

Year: 2019

Venue: Journal of Functional Biomaterials

DOI: https://doi.org/10.3390/jfb10030039

Paper A Thermostable Aspergillus fumigatus GH7 Endoglucanase Over-Expressed in Pichia pastoris Stimulates Lignocellulosic Biomass Hydrolysis

Bernardi, Aline Vianna; Yonamine, Deborah Kimie; Uyemura, Sérgio Akira et al. · 2019 · International Journal of Molecular Sciences · DOI

Full title: A Thermostable Aspergillus fumigatus GH7 Endoglucanase Over-Expressed in Pichia pastoris Stimulates Lignocellulosic Biomass Hydrolysis

Authors: Bernardi, Aline Vianna; Yonamine, Deborah Kimie; Uyemura, Sérgio Akira; Dinamarco, Taísa Magnani

Year: 2019

Venue: International Journal of Molecular Sciences

DOI: https://doi.org/10.3390/ijms20092261

Paper Structural Homology Fails to Predict Secretion Efficiency in Pichia pastoris: Divergent Responses of Architecturally Similar scFvs to Multi-Parametric Genetic Engineering

Ningning Wang; Yang Xiao; Xiyu Liu et al. · 2025 · International Journal of Molecular Sciences · DOI

Full title: Structural Homology Fails to Predict Secretion Efficiency in Pichia pastoris: Divergent Responses of Architecturally Similar scFvs to Multi-Parametric Genetic Engineering

Authors: Ningning Wang; Yang Xiao; Xiyu Liu; Yuanqing Li; D. Yu; Jia Guo; Ping Lu; Xiaopeng Zhang

Year: 2025

Venue: International Journal of Molecular Sciences

DOI: https://doi.org/10.3390/ijms26104922

Paper Yeast Genomics and Its Applications in Biotechnological Processes: What Is Our Present and Near Future?

Tullio, Viviana Cristina · 2022 · Journal of Fungi · DOI

Full title: Yeast Genomics and Its Applications in Biotechnological Processes: What Is Our Present and Near Future?

Authors: Tullio, Viviana Cristina

Year: 2022

Venue: Journal of Fungi

DOI: https://doi.org/10.3390/jof8070752

Paper High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris

Zhou, Qinghua; Su, Zhixin; Jiao, Liangcheng et al. · 2019 · International Journal of Molecular Sciences · DOI

Full title: High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris

Authors: Zhou, Qinghua; Su, Zhixin; Jiao, Liangcheng; Wang, Yao; Yang, Kaixin; Li, Wenjuan; Yan, Yunjun

Year: 2019

Venue: International Journal of Molecular Sciences

DOI: https://doi.org/10.3390/ijms21010279

Paper Expression of Lectins in Heterologous Systems

Martínez-Alarcón, Dania; Blanco‐Labra, Alejandro; García-Gasca, Teresa · 2018 · International Journal of Molecular Sciences · DOI

Full title: Expression of Lectins in Heterologous Systems

Authors: Martínez-Alarcón, Dania; Blanco‐Labra, Alejandro; García-Gasca, Teresa

Year: 2018

Venue: International Journal of Molecular Sciences

DOI: https://doi.org/10.3390/ijms19020616

Paper Applications of the Methylotrophic Yeast Komagataella phaffii in the Context of Modern Biotechnology

Moraes, Lídia Maria Pepe de; Marques, Henrique Fetzner; Reis, Viviane Castelo Branco et al. · 2024 · Journal of Fungi · DOI

Full title: Applications of the Methylotrophic Yeast Komagataella phaffii in the Context of Modern Biotechnology

Authors: Moraes, Lídia Maria Pepe de; Marques, Henrique Fetzner; Reis, Viviane Castelo Branco; Coelho, Cíntia Marques; Leitão, Matheus de Castro; Galdino, Alexsandro Sobreira; Souza, Thaís Paiva Porto de; Piva, Luíza Cesca; Pérez, Ana Laura Alfonso; Trichez, Débora; Almeida, João Ricardo Moreira de; Marco, Janice Lisboa De; Torres, Fernando Araripe Gonçalves

Year: 2024

Venue: Journal of Fungi

DOI: https://doi.org/10.3390/jof10060411

Paper Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production

C. Bustos; J. Quezada; Rhonda Veas et al. · 2022 · Metabolites · DOI

Full title: Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production

Authors: C. Bustos; J. Quezada; Rhonda Veas; C. Altamirano; S. Braun-Galleani; P. Fickers; J. Berrios

Year: 2022

Venue: Metabolites

DOI: https://doi.org/10.3390/metabo12040346

Paper Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins

Попова, Л. Г.; Khramov, Dmitrii E.; Nedelyaeva, Olga I. et al. · 2023 · International Journal of Molecular Sciences · DOI

Full title: Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins

Authors: Попова, Л. Г.; Khramov, Dmitrii E.; Nedelyaeva, Olga I.; Volkov, Vadim

Year: 2023

Venue: International Journal of Molecular Sciences

DOI: https://doi.org/10.3390/ijms241310768

Paper Bioengineered Enzymes and Precision Fermentation in the Food Industry

Boukid, Fatma; Ganeshan, S.; Wang, Yingxin et al. · 2023 · International Journal of Molecular Sciences · DOI

Full title: Bioengineered Enzymes and Precision Fermentation in the Food Industry

Authors: Boukid, Fatma; Ganeshan, S.; Wang, Yingxin; Tülbek, Mehmet; Nickerson, Michael T.

Year: 2023

Venue: International Journal of Molecular Sciences

DOI: https://doi.org/10.3390/ijms241210156

Paper Extracellular Microbial Metabolomics: The State of the Art

Pinu, Farhana R.; Villas‐Bôas, Silas G. · 2017 · Metabolites · DOI

Full title: Extracellular Microbial Metabolomics: The State of the Art

Authors: Pinu, Farhana R.; Villas‐Bôas, Silas G.

Year: 2017

Venue: Metabolites

DOI: https://doi.org/10.3390/metabo7030043

Paper Comparison of Yeasts as Hosts for Recombinant Protein Production

Gomes, Antônio Milton Vieira; Carmo, Talita Souza; Carvalho, Lucas Silva et al. · 2018 · Microorganisms · DOI

Full title: Comparison of Yeasts as Hosts for Recombinant Protein Production

Authors: Gomes, Antônio Milton Vieira; Carmo, Talita Souza; Carvalho, Lucas Silva; Bahia, Frederico Mendonça; Parachin, Nádia Skorupa

Year: 2018

Venue: Microorganisms

DOI: https://doi.org/10.3390/microorganisms6020038

Paper Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

J. Zha; Dan Liu; Juan Ren et al. · 2023 · Journal of Fungi · DOI

Full title: Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

Authors: J. Zha; Dan Liu; Juan Ren; Zhijun Liu; Xia Wu

Year: 2023

Venue: Journal of Fungi

DOI: https://doi.org/10.3390/jof9101027

Paper Isolation and Characterization of the Diatom Phaeodactylum Δ5-Elongase Gene for Transgenic LC-PUFA Production in Pichia pastoris

Jiang, Mulan; Guo, Bing; Wan, Xia et al. · 2014 · Marine Drugs · DOI

Full title: Isolation and Characterization of the Diatom Phaeodactylum Δ5-Elongase Gene for Transgenic LC-PUFA Production in Pichia pastoris

Authors: Jiang, Mulan; Guo, Bing; Wan, Xia; Gong, Yangmin; Zhang, Yinbo; Hu, Chuanjiong

Year: 2014

Venue: Marine Drugs

DOI: https://doi.org/10.3390/md12031317

Paper Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris

Jordà, Joel; Rojas, Hugo; Carnicer, Marc et al. · 2014 · Metabolites · DOI

Full title: Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris

Authors: Jordà, Joel; Rojas, Hugo; Carnicer, Marc; Wahl, A.; Ferrer, Pau; Albiol, Joan

Year: 2014

Venue: Metabolites

DOI: https://doi.org/10.3390/metabo4020281

Paper Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms

Peebo, Karl; Neubauer, Peter · 2018 · Microorganisms · DOI

Full title: Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms

Authors: Peebo, Karl; Neubauer, Peter

Year: 2018

Venue: Microorganisms

DOI: https://doi.org/10.3390/microorganisms6030056

Paper Laccase Gene Family in Cerrena sp. HYB07: Sequences, Heterologous Expression and Transcriptional Analysis

Yang, Jie; Xu, Xinqi; Ng, Tzi Bun et al. · 2016 · Molecules · DOI

Full title: Laccase Gene Family in Cerrena sp. HYB07: Sequences, Heterologous Expression and Transcriptional Analysis

Authors: Yang, Jie; Xu, Xinqi; Ng, Tzi Bun; Lin, Juan; Ye, Xiuyun

Year: 2016

Venue: Molecules

DOI: https://doi.org/10.3390/molecules21081017

Paper Expression of Pleurotus ostreatus Laccase Gene in Pichia pastoris and Its Degradation of Corn Stover Lignin

Song, Qian; Deng, Xun; Song, Ruiqing · 2020 · Microorganisms · DOI

Full title: Expression of Pleurotus ostreatus Laccase Gene in Pichia pastoris and Its Degradation of Corn Stover Lignin

Authors: Song, Qian; Deng, Xun; Song, Ruiqing

Year: 2020

Venue: Microorganisms

DOI: https://doi.org/10.3390/microorganisms8040601

Paper Evolving Paradigms of Recombinant Protein Production in Pharmaceutical Industry: A Rigorous Review

Jayakrishnan, Achuth; Rosli, Wan Rosalina Wan; Tahir, Ahmad Rashidi Mohamed et al. · 2024 · Sci · DOI

Full title: Evolving Paradigms of Recombinant Protein Production in Pharmaceutical Industry: A Rigorous Review

Authors: Jayakrishnan, Achuth; Rosli, Wan Rosalina Wan; Tahir, Ahmad Rashidi Mohamed; Razak, Fashli Syafiq Abd; Kee, Phei Er; Ng, Hui Suan; Chew, Yik-Ling; Lee, Siew-Keah; Ramasamy, Mahenthiran; Tan, Ching Siang; Liew, Kai Bin

Year: 2024

Venue: Sci

DOI: https://doi.org/10.3390/sci6010009

Paper Secretion and activity of antimicrobial peptide cecropin D expressed in Pichia pastoris

Guo, Chunhe; Huang, Yumao; Zheng, Hongyu et al. · 2012 · Experimental and Therapeutic Medicine · DOI

Full title: Secretion and activity of antimicrobial peptide cecropin D expressed in Pichia pastoris

Authors: Guo, Chunhe; Huang, Yumao; Zheng, Hongyu; LiYun, Tang; He, Jun; LinSheng, Xiang; Liu, Dehui; JIANG, HOUQUAN

Year: 2012

Venue: Experimental and Therapeutic Medicine

DOI: https://doi.org/10.3892/etm.2012.719

Paper Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Komagataella phaffii Strains Utilizing Glycerol as Substrate

Melo, Nadielle Tamires Moreira; Pontes, Gabriela Coimbra; Procópio, Dielle Pierotti et al. · 2020 · Microorganisms · DOI

Full title: Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Komagataella phaffii Strains Utilizing Glycerol as Substrate

Authors: Melo, Nadielle Tamires Moreira; Pontes, Gabriela Coimbra; Procópio, Dielle Pierotti; Cunha, Gabriel Caetano de Góis e; Eliodório, Kevy Pontes; Paes, Hugo Costa; Basso, Thiago Olitta; Parachin, Nádia Skorupa

Year: 2020

Venue: Microorganisms

DOI: https://doi.org/10.3390/microorganisms8050781

Paper Design, construction, and in vitro analysis of A33scFv::CDy, a recombinant fusion protein for antibody-directed enzyme prodrug therapy in colon cancer

Coelho, Vânia; Dernedde, Jens; Petrausch, Ulf et al. · 2007 · International Journal of Oncology · DOI

Full title: Design, construction, and in vitro analysis of A33scFv::CDy, a recombinant fusion protein for antibody-directed enzyme prodrug therapy in colon cancer

Authors: Coelho, Vânia; Dernedde, Jens; Petrausch, Ulf; Panjideh, Hossein; Fuchs, Hendrik; Menzel, Christoph; Dübel, Stefan; Keilholz, Ulrich; Thiel, E.; Deckert, P. Markus

Year: 2007

Venue: International Journal of Oncology

DOI: https://doi.org/10.3892/ijo.31.4.951

Paper Expression and purification of FGF21 in Pichia pastoris and its effect on fibroblast-cell migration

Song, Yonghuan; Ding, Jian; Jin, Rilong et al. · 2016 · Molecular Medicine Reports · DOI

Full title: Expression and purification of FGF21 in Pichia pastoris and its effect on fibroblast-cell migration

Authors: Song, Yonghuan; Ding, Jian; Jin, Rilong; Jung, Jin-Hee; Li, Shi; Yang, Jingquan; Wang, Anyuan; Li, Zhijie

Year: 2016

Venue: Molecular Medicine Reports

DOI: https://doi.org/10.3892/mmr.2016.4942

Paper Expression of porcine protegrin-1 in Pichia pastoris and its anticancer activity in vitro

Niu, Mingfu; Chai, Shumao; You, Xiaoyan et al. · 2015 · Experimental and Therapeutic Medicine · DOI

Full title: Expression of porcine protegrin-1 in Pichia pastoris and its anticancer activity in vitro

Authors: Niu, Mingfu; Chai, Shumao; You, Xiaoyan; Wang, Wenhui; Qin, Cuili; Gong, Qiang; Zhang, Tingting; Wan, Peng

Year: 2015

Venue: Experimental and Therapeutic Medicine

DOI: https://doi.org/10.3892/etm.2015.2202

Paper Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin-2 in Pichia pastoris

Xing, Li-Wei; Tian, Shi-Xun; Gao, Wei et al. · 2016 · Experimental and Therapeutic Medicine · DOI

Full title: Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin-2 in Pichia pastoris

Authors: Xing, Li-Wei; Tian, Shi-Xun; Gao, Wei; Yang, Na; Qu, Pei; Liu, Di; Jiao, Jian; Wang, Jue; Feng, Xingjun

Year: 2016

Venue: Experimental and Therapeutic Medicine

DOI: https://doi.org/10.3892/etm.2016.3578

Paper Cholera Toxin Subunit B as Adjuvant––An Accelerator in Protective Immunity and a Break in Autoimmunity

Stratmann, Thomas · 2015 · Vaccines · DOI

Full title: Cholera Toxin Subunit B as Adjuvant––An Accelerator in Protective Immunity and a Break in Autoimmunity

Authors: Stratmann, Thomas

Year: 2015

Venue: Vaccines

DOI: https://doi.org/10.3390/vaccines3030579

Paper Secretion and expression dynamics of a GFP-tagged mucin-type fusion protein in high cell density Pichia pastoris bioreactor cultivations

M. Sjöblom; L. Lindberg; J. Holgersson et al. · 2012 · DOI

Full title: Secretion and expression dynamics of a GFP-tagged mucin-type fusion protein in high cell density Pichia pastoris bioreactor cultivations

Authors: M. Sjöblom; L. Lindberg; J. Holgersson; U. Rova

Year: 2012

DOI: https://doi.org/10.4236/abb.2012.33033

Paper Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Durmusoglu, Deniz; Al’Abri, Ibrahim S.; Li, Zidan et al. · 2023 · Microbial Cell Factories · DOI

Full title: Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Authors: Durmusoglu, Deniz; Al’Abri, Ibrahim S.; Li, Zidan; Williams, Taufika Islam; Collins, Leonard B.; Martínez, José L.; Crook, Nathan

Year: 2023

Venue: Microbial Cell Factories

DOI: https://doi.org/10.1186/s12934-023-02117-y

Paper Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability

Kangwa, Martin; Salgado, José Antonio Gama; Fernández‐Lahore, Marcelo · 2018 · AMB Express · DOI

Full title: Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability

Authors: Kangwa, Martin; Salgado, José Antonio Gama; Fernández‐Lahore, Marcelo

Year: 2018

Venue: AMB Express

DOI: https://doi.org/10.1186/s13568-018-0691-3

Paper Recent Advances in the Metabolic Engineering of Yeasts for Ginsenoside Biosynthesis

Chu, Luan Luong; Montecillo, Jake Adolf V.; Bae, Hanhong · 2020 · Frontiers in Bioengineering and Biotechnology · DOI

Full title: Recent Advances in the Metabolic Engineering of Yeasts for Ginsenoside Biosynthesis

Authors: Chu, Luan Luong; Montecillo, Jake Adolf V.; Bae, Hanhong

Year: 2020

Venue: Frontiers in Bioengineering and Biotechnology

DOI: https://doi.org/10.3389/fbioe.2020.00139

Thesis Expression of human epidermal growth factor in pichia pastoris and optimization of fermentation conditions

Eissazadeh, Samira · 2012 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Expression of human epidermal growth factor in pichia pastoris and optimization of fermentation conditions

Authors: Eissazadeh, Samira

Year: 2012

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/31605/1/FBSB%202012%2030R.pdf

Thesis Eplt4 Proteinaceous Elicitor Produced in Pichia pastoris Has a Protective Effect Against Cercosporidium sofinum Infections of Soybean Leaves

Wang, Y; Song, J; Wu, Y et al. · 2021 · University of Melbourne · DOI

Full title: Eplt4 Proteinaceous Elicitor Produced in Pichia pastoris Has a Protective Effect Against Cercosporidium sofinum Infections of Soybean Leaves

Authors: Wang, Y; Song, J; Wu, Y; Odeph, M; Liu, Z; Howlett, BJ; Wang, S; Yang, P; Yao, L; Zhao, L; Yang, Q

Year: 2021

Venue: University of Melbourne

DOI: https://hdl.handle.net/11343/283084

Thesis Development of yeast expression systems for producing thermostable T1 lipase

Oslan, Siti Nurbaya · 2012 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Development of yeast expression systems for producing thermostable T1 lipase

Authors: Oslan, Siti Nurbaya

Year: 2012

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/33480/1/FBSB%202012%2026R.pdf

Thesis Expression of recombinant thermostable W200R protease in Pichia pastoris

Mohamad Sultan, Muhamad Zarir · 2011 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Expression of recombinant thermostable W200R protease in Pichia pastoris

Authors: Mohamad Sultan, Muhamad Zarir

Year: 2011

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/67162/1/IB%202012%2028%20IR.pdf

Thesis Development of protease deficient Meyerozyma guilliermondii strain so for overexpression of thermostable T1 lipase

Lorrine, Okojie Eseoghene · 2022 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Development of protease deficient Meyerozyma guilliermondii strain so for overexpression of thermostable T1 lipase

Authors: Lorrine, Okojie Eseoghene

Year: 2022

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/104584/1/FBSB%202022%2010%20IR.pdf

Thesis Molecular characterization and expression of H5, N1 and NS1 recombinant proteins of avian influenza virus subtype H5N1 in Pichia pastoris

Abubakar, Mustapha Bala · 2011 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Molecular characterization and expression of H5, N1 and NS1 recombinant proteins of avian influenza virus subtype H5N1 in Pichia pastoris

Authors: Abubakar, Mustapha Bala

Year: 2011

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/70100/1/FPV%202011%2019%20-%20IR.pdf

Thesis Production of thermostable L2 lipase using recombinant Pichia pastoris

Shahidan, Noor Hidayah · 2012 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Production of thermostable L2 lipase using recombinant Pichia pastoris

Authors: Shahidan, Noor Hidayah

Year: 2012

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/32372/1/IB%202012%207R%20pdf.pdf

Thesis Expression And Characterization Of Recombinant Thermostable L2 Lipase In Pichia Pastoris

Sabri, Suriana · 2007 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Expression And Characterization Of Recombinant Thermostable L2 Lipase In Pichia Pastoris

Authors: Sabri, Suriana

Year: 2007

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/4857/1/FBSB_2007_1.pdf

Thesis Enhancing recombinant T1 lipase production in Pichia guilliermondii

Ladidi, Abu Mary · 2017 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Enhancing recombinant T1 lipase production in Pichia guilliermondii

Authors: Ladidi, Abu Mary

Year: 2017

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/70201/1/FBSB%202017%2019%20-%20IR.pdf

Thesis Establishment of optimise strategies in production of recombinant Hepatitis B surface antigen for chip-based antibody detection approach

Tam, Yew Joon · 2015 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Establishment of optimise strategies in production of recombinant Hepatitis B surface antigen for chip-based antibody detection approach

Authors: Tam, Yew Joon

Year: 2015

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/64029/1/IB%202015%2011.pdf

Thesis Expression of Alkaline Protease from Bacillus Stearothermophilus F1 in Pichia Pastoris

Ahmad Latiffi, Amaliawati · 2008 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Expression of Alkaline Protease from Bacillus Stearothermophilus F1 in Pichia Pastoris

Authors: Ahmad Latiffi, Amaliawati

Year: 2008

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/5394/1/IB_2008_6A.pdf

Thesis Structural investigation of alcohol oxidase from Meyerozyma guilliermondii and the use of its promoter for recombinant protein expression

Mahyon, Nur Iznida · 2017 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Structural investigation of alcohol oxidase from Meyerozyma guilliermondii and the use of its promoter for recombinant protein expression

Authors: Mahyon, Nur Iznida

Year: 2017

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/84634/1/FBSB%202019%2017%20-%20ir.pdf

Thesis Expression of recombinant geobacillus SP a-amylase in pichia pastoris GS 115

Gandhi, Sivasangkary · 2014 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Expression of recombinant geobacillus SP a-amylase in pichia pastoris GS 115

Authors: Gandhi, Sivasangkary

Year: 2014

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/56821/1/IB%202014%2018.pdf

Thesis HSF-1, HIF-1and HSP90 expression on recombinant pichia pastoris under fed-batch fermentation

Farías, Jorge G · 2014 · Universidade de São Paulo – USP Digital Library · DOI

Full title: HSF-1, HIF-1and HSP90 expression on recombinant pichia pastoris under fed-batch fermentation

Authors: Farías, Jorge G

Year: 2014

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.scielo.br/pdf/bjm/v45n2/15.pdf

Thesis Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris

Farías, Jorge G · 2014 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris

Authors: Farías, Jorge G

Year: 2014

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.scielo.br/pdf/bjm/v45n2/14.pdf

Thesis Applications of recombinant Pichia pastoris in the healthcare industry

Farías, Jorge G · 2013 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Applications of recombinant Pichia pastoris in the healthcare industry

Authors: Farías, Jorge G

Year: 2013

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.scielo.br/pdf/bjm/v44n4/04.pdf

Thesis Fed-Batch Production of Saccharomyces cerevisiae L-Asparaginase II by Recombinant Pichia pastoris MUTs Strain

Pessoa Junior, Adalberto · 2019 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Fed-Batch Production of Saccharomyces cerevisiae L-Asparaginase II by Recombinant Pichia pastoris MUTs Strain

Authors: Pessoa Junior, Adalberto

Year: 2019

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://doi.org/10.3389/fbioe.2019.00016

Thesis Advances in the regulation and production of fungal enzymes by transcriptomics, proteomics and recombinant strains design. [Editorial]

Segato, Fernando · 2019 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Advances in the regulation and production of fungal enzymes by transcriptomics, proteomics and recombinant strains design. [Editorial]

Authors: Segato, Fernando

Year: 2019

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://doi.org/10.3389/fbioe.2019.00157

Thesis Systems and synthetic biology approaches to engineer fungi for fine chemical production

Silva-Rocha, Rafael · 2018 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Systems and synthetic biology approaches to engineer fungi for fine chemical production

Authors: Silva-Rocha, Rafael

Year: 2018

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://doi.org/10.3389/fbioe.2018.00117

Thesis Yeasts as biopharmaceutical production platforms

Courdavault, Vincent · 2021 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Yeasts as biopharmaceutical production platforms

Authors: Courdavault, Vincent

Year: 2021

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://doi.org/10.3389/ffunb.2021.733492

Thesis Expression and Mutagenesis studies of Candida antactica lipase B

Rotticci-Mulder, Johanna C. · 2003 · KTH Royal Institute of Technology · DOI

Full title: Expression and Mutagenesis studies of Candida antactica lipase B

Authors: Rotticci-Mulder, Johanna C.

Year: 2003

Venue: KTH Royal Institute of Technology

DOI: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3493

Thesis Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris.

Canales, M.; Perez de la Lastra, J.M.; Naranjo, V. et al. · 2009 · Utrecht University · DOI

Full title: Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris.

Authors: Canales, M.; Perez de la Lastra, J.M.; Naranjo, V.; Nijhof, A.M.; Hope, M.; Jongejan, F.; de la Fuente, J.

Year: 2009

Venue: Utrecht University

DOI: https://dspace.library.uu.nl/handle/1874/37475

Thesis High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG

Blanchard, Véronique; Gadkari, R.P; George, A.V.E. et al. · 2008 · Utrecht University · DOI

Full title: High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG

Authors: Blanchard, Véronique; Gadkari, R.P; George, A.V.E.; Roy, R.; Gerwig, G.J.; Leeflang, B.R.; Dighe, R.R.; Boelens, R.; Kamerling, J.P.

Year: 2008

Venue: Utrecht University

DOI: https://dspace.library.uu.nl/handle/1874/29965

Thesis Comparative genomics and transcriptomics of Pichia pastoris

Priest, Margaret; Borowsky, Jonathan; Young, Sarah K. et al. · 2017 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Comparative genomics and transcriptomics of Pichia pastoris

Authors: Priest, Margaret; Borowsky, Jonathan; Young, Sarah K.; Love, Kerry R.; Love, John C; Wu, Jie; Ma, Duanduan; Shah, Kartik A; Bartlett, Mary Catherine; Leeson, Rachel; Whittaker, Charles A.

Year: 2017

Venue: Massachusetts Institute of Technology (MIT)

DOI: http://hdl.handle.net/1721.1/107984

Thesis A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

Lee, Kevin S.; Lee, Harry L. T.; Mozdzierz, Nicholas Joseph et al. · 2017 · Massachusetts Institute of Technology (MIT) · DOI

Full title: A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

Authors: Lee, Kevin S.; Lee, Harry L. T.; Mozdzierz, Nicholas Joseph; Love, Kerry R.; Shah, Kartik A; Ram, Rajeev J; Love, John C

Year: 2017

Venue: Massachusetts Institute of Technology (MIT)

DOI: http://hdl.handle.net/1721.1/109453

Thesis Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris

Shah, Kartik A; Clark, John J; Goods, Brittany A. et al. · 2017 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris

Authors: Shah, Kartik A; Clark, John J; Goods, Brittany A.; Politano, Timothy James; Mozdzierz, Nicholas Joseph; Zimnisky, Ross; Leeson, Rachel; Love, John C; Love, Kerry R.

Year: 2017

Venue: Massachusetts Institute of Technology (MIT)

DOI: http://hdl.handle.net/1721.1/109438

Thesis Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care

Cleto, Sara; Lee, Kevin; Perez-Pinera, Pablo et al. · 2017 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care

Authors: Cleto, Sara; Lee, Kevin; Perez-Pinera, Pablo; Han, Ningren; Cao, Jicong; Purcell, Oliver; Shah, Kartik A; Ram, Rajeev J; Lu, Timothy K

Year: 2017

Venue: Massachusetts Institute of Technology (MIT)

DOI: http://hdl.handle.net/1721.1/109076

Thesis Eukaryotic expression: developments for structural proteomics

Aricescu, A. R.; Assenberg, R.; Bill, R. M. et al. · 2006 · Utrecht University · DOI

Full title: Eukaryotic expression: developments for structural proteomics

Authors: Aricescu, A. R.; Assenberg, R.; Bill, R. M.; Busso, D.; Chang, V. T.; Davis, S. J.; Dubrovsky, A.; Gustafsson, L.L.; Hedfalk, K.; Heinemann, U.; Jones, I. M.; Ksiazek, D.; Lang, Chim C; Maskos, K.; Messerschmidt, A.; Macieira, S.; Peleg, Y.; Perrakis, A.; Poterszman, A.; Schneider, G.; Sixma, Titia K; Sussman, Joel L.; Sutton, G.; Tarboureich, N.; Zeev-Ben-Mordehai, T.; Jones, E. Yvonne

Year: 2006

Venue: Utrecht University

DOI: https://dspace.library.uu.nl/handle/1874/357756

Thesis Establishment of a novel Pichia Pastoris host production platform

Coleman, Ellen(Ellen M.) · 2020 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Establishment of a novel Pichia Pastoris host production platform

Authors: Coleman, Ellen(Ellen M.)

Year: 2020

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/126948

Thesis Development of a platform process for the production and purification of single‐domain antibodies

Crowell, Laura E; Goodwine, Chaz; Holt, Carla S et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Development of a platform process for the production and purification of single‐domain antibodies

Authors: Crowell, Laura E; Goodwine, Chaz; Holt, Carla S; Rocha, Lucia; Vega, Celina; Rodriguez, Sergio A; Dalvie, Neil C; Tracey, Mary K; Puntel, Mariana; Wigdorovitz, Andrés; Parreño, Viviana; Love, Kerry R; Cramer, Steven M; Love, J Christopher

Year: 2021

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/135524

Thesis Macroscopic modeling of bioreactors for recombinant protein producing Pichia pastoris in defined medium

Hong, Moo Sun; Velez-Suberbie, M Lourdes; Maloney, Andrew J et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Macroscopic modeling of bioreactors for recombinant protein producing Pichia pastoris in defined medium

Authors: Hong, Moo Sun; Velez-Suberbie, M Lourdes; Maloney, Andrew J; Biedermann, Andrew; Love, Kerry R; Love, J Christopher; Mukhopadhyay, Tarit K; Braatz, Richard D

Year: 2021

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/133351

Thesis Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain

Brady, Joseph R; Whittaker, Charles A; Tan, Melody C et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain

Authors: Brady, Joseph R; Whittaker, Charles A; Tan, Melody C; Kristensen, D Lee; Ma, Duanduan; Dalvie, Neil C; Love, Kerry Routenberg; Love, J Christopher

Year: 2021

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/136502

Thesis CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome

Dalvie, Neil C.; Lorgeree, Timothy R.; Yang, Yuchen et al. · 2024 · Massachusetts Institute of Technology (MIT) · DOI

Full title: CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome

Authors: Dalvie, Neil C.; Lorgeree, Timothy R.; Yang, Yuchen; Rodriguez-Aponte, Sergio A.; Whittaker, Charles A.; Hinckley, Joshua A.; Clark, John J.; Del Rosario, Amanda M.; Love, Kerry R.; Love, J. C.

Year: 2024

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/155951

Thesis Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii

Dalvie, Neil C.; Leal, Justin; Whittaker, Charles A. et al. · 2022 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii

Authors: Dalvie, Neil C.; Leal, Justin; Whittaker, Charles A.; Yang, Yuchen; Brady, Joseph R.; Love, Kerry R.; Love, J. Christopher

Year: 2022

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/136627.2

Thesis Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

Dalvie, Neil C.; Naranjo, Christopher A.; Rodriguez-Aponte, Sergio A. et al. · 2022 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

Authors: Dalvie, Neil C.; Naranjo, Christopher A.; Rodriguez-Aponte, Sergio A.; Johnston, Ryan S.; Christopher Love, J.

Year: 2022

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/145353

Thesis Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

Dalvie, Neil C; Brady, Joseph R; Crowell, Laura E et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

Authors: Dalvie, Neil C; Brady, Joseph R; Crowell, Laura E; Tracey, Mary K; Biedermann, Andrew M; Kaur, Kawaljit; Hickey, John M; Kristensen, D. L; Bonnyman, Alexandra D; Rodriguez-Aponte, Sergio A; Whittaker, Charles A; Bok, Marina; Vega, Celina; Mukhopadhyay, Tarit K; Joshi, Sangeeta B; Volkin, David B

Year: 2021

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/136780

Thesis Enhanced Expression of Alcohol Dehydrogenase I in Pichia pastoris Reduces the Content of Acetaldehyde in Wines

Geng, Kun; Lin, Ying; Zheng, Xueyun et al. · 2024 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Enhanced Expression of Alcohol Dehydrogenase I in Pichia pastoris Reduces the Content of Acetaldehyde in Wines

Authors: Geng, Kun; Lin, Ying; Zheng, Xueyun; Li, Cheng; Chen, Shuting; Ling, He; Yang, Jun; Zhu, Xiangyu; Liang, Shuli

Year: 2024

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/153421

Thesis Adaptation of Aglycosylated Monoclonal Antibodies for Improved Production in Komagataella phaffii

Yang, Yuchen; Dalvie, Neil C; Brady, Joseph R et al. · 2025 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Adaptation of Aglycosylated Monoclonal Antibodies for Improved Production in Komagataella phaffii

Authors: Yang, Yuchen; Dalvie, Neil C; Brady, Joseph R; Naranjo, Christopher A; Lorgeree, Timothy; Rodriguez‐Aponte, Sergio A; Johnston, Ryan S; Tracey, Mary K; Elenberger, Carmen M; Lee, Eric; Tié, Mark; Love, Kerry R; Love, J Christopher

Year: 2025

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/163225

Thesis Systematic Single-Cell Analysis of Pichia pastoris Reveals Secretory Capacity Limits Productivity

Politano, Timothy James; Panagiotou, Vasiliki; Stadheim, Terrance A. et al. · 2012 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Systematic Single-Cell Analysis of Pichia pastoris Reveals Secretory Capacity Limits Productivity

Authors: Politano, Timothy James; Panagiotou, Vasiliki; Stadheim, Terrance A.; Love, Kerry R.; Jiang, Bo, S.M. Massachusetts Institute of Technology; Love, John C

Year: 2012

Venue: Massachusetts Institute of Technology (MIT)

DOI: http://hdl.handle.net/1721.1/72414

Thesis A central composite design to investigate antibody fragment production by Pichia pastoris

Chan, Joyce, M. Eng. Massachusetts Institute of Technology · 2006 · Massachusetts Institute of Technology (MIT) · DOI

Full title: A central composite design to investigate antibody fragment production by Pichia pastoris

Authors: Chan, Joyce, M. Eng. Massachusetts Institute of Technology

Year: 2006

Venue: Massachusetts Institute of Technology (MIT)

DOI: http://hdl.handle.net/1721.1/33872

Thesis Clonal selection and characterization of epigenetic variation in Pichia pastoris

Panagiotou, Vasiliki · 2010 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Clonal selection and characterization of epigenetic variation in Pichia pastoris

Authors: Panagiotou, Vasiliki

Year: 2010

Venue: Massachusetts Institute of Technology (MIT)

DOI: http://hdl.handle.net/1721.1/59881

Thesis Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.

Love, Kerry Routenberg; Panagiotou, Vasiliki; Jiang, Bo et al. · 2012 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.

Authors: Love, Kerry Routenberg; Panagiotou, Vasiliki; Jiang, Bo; Stadheim, Terrance A.; Love, J. Christopher

Year: 2012

Venue: Massachusetts Institute of Technology (MIT)

DOI: http://hdl.handle.net/1721.1/69920

Thesis Targeted detection of secretion pathway protein interactions for therapeutic production and cellular engineering

Robinson, Caressa Mystique · 2020 · University of California, San Diego (UCSD) · DOI

Full title: Targeted detection of secretion pathway protein interactions for therapeutic production and cellular engineering

Authors: Robinson, Caressa Mystique

Year: 2020

Venue: University of California, San Diego (UCSD)

DOI: https://escholarship.org/uc/item/4q15r9h9

Thesis A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable

Leynaud-Kieffer, Laure M. C.; Curran, Samuel C.; Kim, Irene et al. · 2019 · École polytechnique fédérale de Lausanne (EPFL) · DOI

Full title: A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable

Authors: Leynaud-Kieffer, Laure M. C.; Curran, Samuel C.; Kim, Irene; Magnuson, Jon K.; Gladden, John M.; Baker, Scott E.; Simmons, Blake A.

Year: 2019

Venue: École polytechnique fédérale de Lausanne (EPFL)

DOI: https://infoscience.epfl.ch/handle/20.500.14299/157981

Thesis Produção biotecnológica de L-asparaginase (ASP1) de Saccharomyces cerevisiae em sistema de expressão heterólogo Pichia pastoris

Monteiro, Gisele · 2015 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Produção biotecnológica de L-asparaginase (ASP1) de Saccharomyces cerevisiae em sistema de expressão heterólogo Pichia pastoris

Authors: Monteiro, Gisele

Year: 2015

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.teses.usp.br/teses/disponiveis/9/9134/tde-15122015-172127/

Thesis Expressão de fragmentos variáveis de cadeia simples anti-LDL eletronegativa (scFv) em Pichia pastoris e seu efeito sobre a formação de células espumosas

Abdalla, Dulcinéia Saes Parra · 2010 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Expressão de fragmentos variáveis de cadeia simples anti-LDL eletronegativa (scFv) em Pichia pastoris e seu efeito sobre a formação de células espumosas

Authors: Abdalla, Dulcinéia Saes Parra

Year: 2010

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.teses.usp.br/teses/disponiveis/9/9136/tde-08072010-141439/

Thesis Clonagem e expressão heteróloga do gene de uma xilanase de Thermoascus aurantiacus em Pichia pastoris

Franco, Fernanda Craveiro [UNESP] · 2014 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Clonagem e expressão heteróloga do gene de uma xilanase de Thermoascus aurantiacus em Pichia pastoris

Authors: Franco, Fernanda Craveiro [UNESP]

Year: 2014

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://hdl.handle.net/11449/94847

Thesis An improved process for the production of highly purified recombinant thaumatin tagged-variants

Healey, RD; Lebhar, H; https://orcid.org/0000-0002-9023-2467 et al. · 2017 · UNSW Sydney · DOI

Full title: An improved process for the production of highly purified recombinant thaumatin tagged-variants

Authors: Healey, RD; Lebhar, H ; https://orcid.org/0000-0002-9023-2467; Hornung, S; Thordarson, P ; https://orcid.org/0000-0002-1200-8814; Marquis, CP ; https://orcid.org/0000-0003-1656-7215

Year: 2017

Venue: UNSW Sydney

DOI: http://hdl.handle.net/1959.4/unsworks_73890

Thesis Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris.

Gurkan, Cemal; Ellar, David J · 2011 · University of Cambridge · DOI

Full title: Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris.

Authors: Gurkan, Cemal; Ellar, David J

Year: 2011

Venue: University of Cambridge

DOI: http://www.dspace.cam.ac.uk/handle/1810/238082

Thesis Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris.

Whyteside, Graham; Alcocer, Marcos JC; Kumita, Janet R et al. · 2020 · University of Cambridge · DOI

Full title: Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris.

Authors: Whyteside, Graham; Alcocer, Marcos JC; Kumita, Janet R; Dobson, Christopher M; Lazarou, Maria; Pleass, Richard J; Archer, David B

Year: 2020

Venue: University of Cambridge

DOI: https://www.repository.cam.ac.uk/handle/1810/309386

Thesis LPS ligand and culture additives improve production of monomeric MD-1 and 2 in Pichia pastoris by decreasing aggregation and intermolecular disulfide bonding.

Mengwasser, Kristen E; Bryant, Clare E; Gay, Nick J et al. · 2016 · University of Cambridge · DOI

Full title: LPS ligand and culture additives improve production of monomeric MD-1 and 2 in Pichia pastoris by decreasing aggregation and intermolecular disulfide bonding.

Authors: Mengwasser, Kristen E; Bryant, Clare E; Gay, Nick J; Gangloff, Monique

Year: 2016

Venue: University of Cambridge

DOI: https://www.repository.cam.ac.uk/handle/1810/260369

Thesis Produção de fragmento recombinante de anticorpo em Pichia pastoris

Pessoa Junior, Adalberto · 2014 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Produção de fragmento recombinante de anticorpo em Pichia pastoris

Authors: Pessoa Junior, Adalberto

Year: 2014

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.teses.usp.br/teses/disponiveis/9/9134/tde-10062014-084638/

Thesis Produção do fragmento de anticorpo scFv por Pichia pastoris geneticamente modificada

Pessoa Junior, Adalberto · 2013 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Produção do fragmento de anticorpo scFv por Pichia pastoris geneticamente modificada

Authors: Pessoa Junior, Adalberto

Year: 2013

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.teses.usp.br/teses/disponiveis/9/9135/tde-28052015-103520/

Thesis Produção biotecnológica de L-asparaginase(ASP3) de Saccharomyces cerevisiae em sistema de expressão heterólogo Pichia pastoris

Monteiro, Gisele · 2015 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Produção biotecnológica de L-asparaginase(ASP3) de Saccharomyces cerevisiae em sistema de expressão heterólogo Pichia pastoris

Authors: Monteiro, Gisele

Year: 2015

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.teses.usp.br/teses/disponiveis/9/9134/tde-15122015-082825/

Thesis Humanização específica do sistema de glicosilação de Pichia pastoris pela técnica CRISPR/Cas9 visando a expressão de glicoproteínas humanas

Ho, Paulo Lee · 2016 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Humanização específica do sistema de glicosilação de Pichia pastoris pela técnica CRISPR/Cas9 visando a expressão de glicoproteínas humanas

Authors: Ho, Paulo Lee

Year: 2016

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-11042017-084657/

Thesis Expressão da L-asparaginase II de Saccharomyces cerevisiae recombinante extracelular com glicosilação humanizada em Pichia pastoris

Monteiro, Gisele · 2019 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Expressão da L-asparaginase II de Saccharomyces cerevisiae recombinante extracelular com glicosilação humanizada em Pichia pastoris

Authors: Monteiro, Gisele

Year: 2019

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.teses.usp.br/teses/disponiveis/9/9134/tde-02102019-150712/

Thesis Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso

Russo, Elisa Maria de Sousa · 2017 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso

Authors: Russo, Elisa Maria de Sousa

Year: 2017

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://www.teses.usp.br/teses/disponiveis/60/60135/tde-23112017-101039/

Thesis Transcriptoma e expressão recombinante de peptidases do fungo Phanerochaete chrysosporium em Pichia pastoris

Cabral, Hamilton · 2019 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Transcriptoma e expressão recombinante de peptidases do fungo Phanerochaete chrysosporium em Pichia pastoris

Authors: Cabral, Hamilton

Year: 2019

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.teses.usp.br/teses/disponiveis/60/60137/tde-18122019-171832/

Thesis Expanding the metabolic engineering toolbox to rationally and predictably modulate gene expression at the transcriptional and translational level

Peters, Gert · 2017 · Ghent University (Universiteit Gent) · DOI

Full title: Expanding the metabolic engineering toolbox to rationally and predictably modulate gene expression at the transcriptional and translational level

Authors: Peters, Gert

Year: 2017

Venue: Ghent University (Universiteit Gent)

DOI: https://biblio.ugent.be/publication/8518664

Thesis Development of molecular tools for Komagataella phaffii and exploring its use for orally administered biopharmaceuticals

Vanluchene, Robin · 2020 · Ghent University (Universiteit Gent) · DOI

Full title: Development of molecular tools for Komagataella phaffii and exploring its use for orally administered biopharmaceuticals

Authors: Vanluchene, Robin

Year: 2020

Venue: Ghent University (Universiteit Gent)

DOI: https://biblio.ugent.be/publication/8684279

Thesis Design of a cultivation medium for protein production in Pichia pastoris based on genome-wide biological understanding

Matthews, Catherine Bartlett. · 2019 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Design of a cultivation medium for protein production in Pichia pastoris based on genome-wide biological understanding

Authors: Matthews, Catherine Bartlett.

Year: 2019

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/122528

Thesis Product and host engineering for low-cost manufacturing of therapeutic proteins in the yeast Komagataella phaffii

Dalvie, Neil Chandra · 2022 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Product and host engineering for low-cost manufacturing of therapeutic proteins in the yeast Komagataella phaffii

Authors: Dalvie, Neil Chandra

Year: 2022

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/144884

Thesis An integrated approach to enable rapid scalable upstream production of subunit vaccines with Pichia pastoris (Komagataella phaffii)

Biedermann, Andrew M. · 2022 · Massachusetts Institute of Technology (MIT) · DOI

Full title: An integrated approach to enable rapid scalable upstream production of subunit vaccines with Pichia pastoris (Komagataella phaffii)

Authors: Biedermann, Andrew M.

Year: 2022

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/144883

Thesis Molecular, Genetic, and Process Approaches for Improving Secreted Pharmaceutical Protein Quality in Komagataella phaffii

Yang, Yuchen · 2024 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Molecular, Genetic, and Process Approaches for Improving Secreted Pharmaceutical Protein Quality in Komagataella phaffii

Authors: Yang, Yuchen

Year: 2024

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/157251

Thesis Kinetics and modeling of protein production in a recombinant yeast containing temperature-regulated promoters /

Shields, Richard William · 1989 · OhioLINK Electronic Theses and Dissertations · DOI

Full title: Kinetics and modeling of protein production in a recombinant yeast containing temperature-regulated promoters /

Authors: Shields, Richard William

Year: 1989

Venue: OhioLINK Electronic Theses and Dissertations

DOI: http://rave.ohiolink.edu/etdc/view?acc_num=osu1749745350688424

Thesis GENERATION, CLONING, AND SMALL-SCALE EXPRESSION OF SITE-DIRECTED MUTANTS OF HEN EGG WHITE LYSOZYME IN PICHIA PASTORIS

Patton, Nichole L. · 2012 · OhioLINK Electronic Theses and Dissertations · DOI

Full title: GENERATION, CLONING, AND SMALL-SCALE EXPRESSION OF SITE-DIRECTED MUTANTS OF HEN EGG WHITE LYSOZYME IN PICHIA PASTORIS

Authors: Patton, Nichole L.

Year: 2012

Venue: OhioLINK Electronic Theses and Dissertations

DOI: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1348846216

Thesis Exploring the dual roles of tRNAs in Komagataella phaffii production hosts

Chahid, Nisreen · 2024 · White Rose eTheses (Leeds, Sheffield, York) · DOI

Full title: Exploring the dual roles of tRNAs in Komagataella phaffii production hosts

Authors: Chahid, Nisreen

Year: 2024

Venue: White Rose eTheses (Leeds, Sheffield, York)

DOI: https://etheses.whiterose.ac.uk/id/eprint/37710/1/Chahid_203013793_CorrectedThesisClean.pdf

Thesis An optimized high throughput platform, from genetic transformation to fermentation, to screen for secreted laccases in Aspergillus niger

Leynaud-Kieffer Curran, Laure Marie Colette · 2022 · École polytechnique fédérale de Lausanne (EPFL) · DOI

Full title: An optimized high throughput platform, from genetic transformation to fermentation, to screen for secreted laccases in Aspergillus niger

Authors: Leynaud-Kieffer Curran, Laure Marie Colette

Year: 2022

Venue: École polytechnique fédérale de Lausanne (EPFL)

DOI: https://infoscience.epfl.ch/handle/20.500.14299/184440

Thesis Quantitative characterization of a recombinant Pichia pastoris Mut+ strain secreting avidin using transient continuous cultures

Jungo, Carmen · 2007 · École polytechnique fédérale de Lausanne (EPFL) · DOI

Full title: Quantitative characterization of a recombinant Pichia pastoris Mut+ strain secreting avidin using transient continuous cultures

Authors: Jungo, Carmen

Year: 2007

Venue: École polytechnique fédérale de Lausanne (EPFL)

DOI: https://infoscience.epfl.ch/handle/20.500.14299/6151

Thesis Bench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris based expression systems

Ramchuran, Santosh · 2005 · Lund University (LUP) · DOI

Full title: Bench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris based expression systems

Authors: Ramchuran, Santosh

Year: 2005

Venue: Lund University (LUP)

DOI: https://lup.lub.lu.se/record/544473

Thesis Pichia pastoris as Enzyme Production Host: from Toolkit Design to Protein Expression

Obst, Ulrike · 2019 · Technical University of Munich (TUM) · DOI

Full title: Pichia pastoris as Enzyme Production Host: from Toolkit Design to Protein Expression

Authors: Obst, Ulrike

Year: 2019

Venue: Technical University of Munich (TUM)

DOI: https://mediatum.ub.tum.de/1462686

Thesis Enhancing Eukaryotic Cell-Free Systems through Genetic Engineering and Optimization of Protein Production Processes

Schloßhauer, Jeffrey Lesslie · 2024 · Freie Universität Berlin (Refubium) · DOI

Full title: Enhancing Eukaryotic Cell-Free Systems through Genetic Engineering and Optimization of Protein Production Processes

Authors: Schloßhauer, Jeffrey Lesslie

Year: 2024

Venue: Freie Universität Berlin (Refubium)

DOI: https://refubium.fu-berlin.de/handle/fub188/45613

Thesis Design, expression, and testing of a bivalent subunit botulism vaccine for use in cattle

Shtevi, Abraham · 2009 · James Cook University · DOI

Full title: Design, expression, and testing of a bivalent subunit botulism vaccine for use in cattle

Authors: Shtevi, Abraham

Year: 2009

Venue: James Cook University

DOI: https://researchonline.jcu.edu.au/11603/13/JCU_11603_Shtevi_2009_thesis.pdf

Thesis Optimization of the production of recombinant macrophage inhibitory cytokine 1 (MIC-1) from Pichia pastoris

Pratanaphon, Ronachai · 2006 · UNSW Sydney · DOI

Full title: Optimization of the production of recombinant macrophage inhibitory cytokine 1 (MIC-1) from Pichia pastoris

Authors: Pratanaphon, Ronachai

Year: 2006

Venue: UNSW Sydney

DOI: http://hdl.handle.net/1959.4/68401

Thesis Improving protein yield from mammalian cells by manipulation of stress response pathways

Chalmers, Fiona · 2016 · University of Glasgow · DOI

Full title: Improving protein yield from mammalian cells by manipulation of stress response pathways

Authors: Chalmers, Fiona

Year: 2016

Venue: University of Glasgow

DOI: https://theses.gla.ac.uk/7666/7/2016ChalmersPhD.pdf

Thesis Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production

Zhang, Wei · 2014 · Virginia Tech - VTechWorks · DOI

Full title: Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production

Authors: Zhang, Wei

Year: 2014

Venue: Virginia Tech - VTechWorks

DOI: http://hdl.handle.net/10919/33395

Thesis Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter

Barrigón de San Marcos, José Manuel · 2015 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter

Authors: Barrigón de San Marcos, José Manuel

Year: 2015

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/287984

Thesis Lipidomics studies of recombinant Pichia pastoris for improved recombinant protein secretion through cell engineering

Adelantado Vallvé, Núria · 2016 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Lipidomics studies of recombinant Pichia pastoris for improved recombinant protein secretion through cell engineering

Authors: Adelantado Vallvé, Núria

Year: 2016

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/384229

Thesis Development of Pichia pastoris for the production of human α-galactosidase A as a model lysosomal enzyme

Barreiro Vázquez, Antonio · 2016 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Development of Pichia pastoris for the production of human α-galactosidase A as a model lysosomal enzyme

Authors: Barreiro Vázquez, Antonio

Year: 2016

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/382822

Thesis A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches

Garcia Ortega, Xavier · 2016 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches

Authors: Garcia Ortega, Xavier

Year: 2016

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/384531

Thesis Anàlisi quantitatiu de l’efecte de l’expressió d’una lipasa recombinant sobre el metabolisme central de Pichia pastoris

Jordà Murria, Joel · 2013 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Anàlisi quantitatiu de l’efecte de l’expressió d’una lipasa recombinant sobre el metabolisme central de Pichia pastoris

Authors: Jordà Murria, Joel

Year: 2013

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/117310

Thesis L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic

Cámara Rey, Elena · 2017 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic

Authors: Cámara Rey, Elena

Year: 2017

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/399833

Thesis Bioprocess efficiency as a roadmap to establish Pichia pastoris as a reliable cell factory

Ponte Font, Xavier · 2017 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Bioprocess efficiency as a roadmap to establish Pichia pastoris as a reliable cell factory

Authors: Ponte Font, Xavier

Year: 2017

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/457712

Thesis Development of the production process for a recombinant hormone and evaluation of its biological activity

Font Ingles, Albert · 2013 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Development of the production process for a recombinant hormone and evaluation of its biological activity

Authors: Font Ingles, Albert

Year: 2013

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/123298

Thesis Developing strategies for systems metabolic engineering of Pichia pastoris

Tomàs Gamisans, Màrius · 2017 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Developing strategies for systems metabolic engineering of Pichia pastoris

Authors: Tomàs Gamisans, Màrius

Year: 2017

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/458538

Thesis Overcoming the secretory limitations in Pichia pastoris for recombinant protein production

Barrero Peña, Juan José · 2021 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Overcoming the secretory limitations in Pichia pastoris for recombinant protein production

Authors: Barrero Peña, Juan José

Year: 2021

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/670387

Thesis Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris

Garrigós Martínez, Javier · 2021 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris

Authors: Garrigós Martínez, Javier

Year: 2021

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/671119

Thesis Estratègies d’operació per a la producció de lipases de Rhizopus oryzae amb el fenotip Mut(s) de Pichia pastoris mitjançant substrats mixtes

Arnau Jiménez, Carol · 2011 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Estratègies d’operació per a la producció de lipases de Rhizopus oryzae amb el fenotip Mut(s) de Pichia pastoris mitjançant substrats mixtes

Authors: Arnau Jiménez, Carol

Year: 2011

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/42007

Thesis Systematic metabolic analysis of recombinant Pichia pastoris under different oxygen conditions A Metabolome and Fluxome Based Study

Carnicer Heras, Marc · 2012 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Systematic metabolic analysis of recombinant Pichia pastoris under different oxygen conditions A Metabolome and Fluxome Based Study

Authors: Carnicer Heras, Marc

Year: 2012

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/96813

Thesis Systems metabolic engineering for recombinant protein production in Pichia pastoris

Monforte Mercado, Sergi · 2020 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Systems metabolic engineering for recombinant protein production in Pichia pastoris

Authors: Monforte Mercado, Sergi

Year: 2020

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/669385

Thesis Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris

Nieto Taype, Miguel Angel · 2020 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris

Authors: Nieto Taype, Miguel Angel

Year: 2020

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/670163

Thesis Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess

Gasset Franch, Arnau · 2024 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess

Authors: Gasset Franch, Arnau

Year: 2024

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/691293

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Metadata only

Paper Single‐chain antibody fragment production in Pichia pastoris: Benefits of prolonged pre‐induction glycerol feeding

N. K. Khatri; D. Gocke; Oliver Trentmann et al. · 2011 · Biotechnology Journal · DOI

Full title: Single‐chain antibody fragment production in Pichia pastoris: Benefits of prolonged pre‐induction glycerol feeding

Authors: N. K. Khatri; D. Gocke; Oliver Trentmann; P. Neubauer; F. Hoffmann

Year: 2011

Venue: Biotechnology Journal

DOI: https://doi.org/10.1002/biot.201000193

Paper Customizing amino acid metabolism of Pichia pastoris for recombinant protein production

Rußmayer, Hannes; Buchetics, Markus; Mattanovich, Matthias et al. · 2023 · Biotechnology Journal · DOI

Full title: Customizing amino acid metabolism of Pichia pastoris for recombinant protein production

Authors: Rußmayer, Hannes; Buchetics, Markus; Mattanovich, Matthias; Neubauer, Stefan; Steiger, Matthias G.; Graf, Alexandra B.; Koellensperger, Gunda; Hann, Stephan; Sauer, Michael; Gasser, Brigitte; Mattanovich, Diethard

Year: 2023

Venue: Biotechnology Journal

DOI: https://doi.org/10.1002/biot.202300033

Paper Biosynthesis of High‐Active Hemoproteins by the Efficient Heme‐Supply Pichia Pastoris Chassis

Yu, Fei; Zhao, Xinrui; Zhou, Jingwen et al. · 2023 · Advanced Science · DOI

Full title: Biosynthesis of High‐Active Hemoproteins by the Efficient Heme‐Supply Pichia Pastoris Chassis

Authors: Yu, Fei; Zhao, Xinrui; Zhou, Jingwen; Lü, Wei; Li, Jianghua; Chen, Jian; Du, Guocheng

Year: 2023

Venue: Advanced Science

DOI: https://doi.org/10.1002/advs.202302826

Paper Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space‐time yields for less complex proteins

Maccani, Andreas; Landes, Nils; Stadlmayr, Gerhard et al. · 2014 · Biotechnology Journal · DOI

Full title: Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space‐time yields for less complex proteins

Authors: Maccani, Andreas; Landes, Nils; Stadlmayr, Gerhard; Maresch, Daniel; Leitner, Christian; Maurer, Michael; Gasser, Brigitte; Ernst, Wolfgang; Kunert, Renate; Mattanovich, Diethard

Year: 2014

Venue: Biotechnology Journal

DOI: https://doi.org/10.1002/biot.201300305

Paper Genome‐scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production

Sohn, Seung Bum; Graf, Alexandra B.; Kim, Tae Yong et al. · 2010 · Biotechnology Journal · DOI

Full title: Genome‐scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production

Authors: Sohn, Seung Bum; Graf, Alexandra B.; Kim, Tae Yong; Gasser, Brigitte; Maurer, Michael; Ferrer, Pau; Mattanovich, Diethard; Lee, Sang Yup

Year: 2010

Venue: Biotechnology Journal

DOI: https://doi.org/10.1002/biot.201000078

Paper RSM optimization of HSA/IL1Ra in Pichia pastoris overexpression strain and study of its in vivo activity in reducing hyperglycemia of GK rats.

Liu, Wenhui; Zhao, Wenbin; Lai, Jun et al. · 2017 · Biotechnology and applied biochemistry · DOI

Full title: RSM optimization of HSA/IL1Ra in Pichia pastoris overexpression strain and study of its in vivo activity in reducing hyperglycemia of GK rats.

Authors: Liu, Wenhui; Zhao, Wenbin; Lai, Jun; Shen, Qi; Xu, Yingchun; Pan, Liqiang; Chen, Shuqing

Year: 2017

Venue: Biotechnology and applied biochemistry

DOI: https://doi.org/10.1002/bab.1532

Paper Codon harmonization – going beyond the speed limit for protein expression

Mignon, Charlotte; Mariano, Natacha; Stadthagen, Gustavo et al. · 2018 · FEBS Letters · DOI

Full title: Codon harmonization – going beyond the speed limit for protein expression

Authors: Mignon, Charlotte; Mariano, Natacha; Stadthagen, Gustavo; Lugari, Adrien; Lagoutte, Priscillia; Donnat, Stéphanie; Chenavas, Sylvie; Perot, Cyril; Sodoyer, Régis; Werle, Bettina

Year: 2018

Venue: FEBS Letters

DOI: https://doi.org/10.1002/1873-3468.13046

Paper Industrial biotechnology: Tools and applications

Tang, Weng Lin; Zhao, Huimin · 2009 · Biotechnology Journal · DOI

Full title: Industrial biotechnology: Tools and applications

Authors: Tang, Weng Lin; Zhao, Huimin

Year: 2009

Venue: Biotechnology Journal

DOI: https://doi.org/10.1002/biot.200900127

Paper Engineered Deregulation of Expression in Yeast with Designed Hybrid‐Promoter Architectures in Coordination with Discovered Master Regulator Transcription Factor

Burcu Gündüz Ergün; Irem Demir; T. Özdamar et al. · 2020 · Advanced Biosystems · DOI

Full title: Engineered Deregulation of Expression in Yeast with Designed Hybrid‐Promoter Architectures in Coordination with Discovered Master Regulator Transcription Factor

Authors: Burcu Gündüz Ergün; Irem Demir; T. Özdamar; Brigitte Gasser; D. Mattanovich; P. Çalık

Year: 2020

Venue: Advanced Biosystems

DOI: https://doi.org/10.1002/adbi.201900172

Paper The Degree and Length of O‐Glycosylation of Recombinant Proteins Produced in Pichia pastoris Depends on the Nature of the Protein and the Process Type

Radoman, Bojana; Grünwald‐Gruber, Clemens; Schmelzer, Bernhard et al. · 2020 · Biotechnology Journal · DOI

Full title: The Degree and Length of O‐Glycosylation of Recombinant Proteins Produced in Pichia pastoris Depends on the Nature of the Protein and the Process Type

Authors: Radoman, Bojana; Grünwald‐Gruber, Clemens; Schmelzer, Bernhard; Zavec, Domen; Gasser, Brigitte; Altmann, Friedrich; Mattanovich, Diethard

Year: 2020

Venue: Biotechnology Journal

DOI: https://doi.org/10.1002/biot.202000266

Paper Transcriptional engineering of the glyceraldehyde‐3‐phosphate dehydrogenase promoter for improved heterologous protein production in Pichia pastoris

Özge Ata; R. Prielhofer; Brigitte Gasser et al. · 2017 · Biotechnology and Bioengineering · DOI

Full title: Transcriptional engineering of the glyceraldehyde‐3‐phosphate dehydrogenase promoter for improved heterologous protein production in Pichia pastoris

Authors: Özge Ata; R. Prielhofer; Brigitte Gasser; D. Mattanovich; P. Çalık

Year: 2017

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.26363

Paper Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris

Hubertus Hohenblum; Brigitte Gasser; Michael Maurer et al. · 2004 · Biotechnology and Bioengineering · DOI

Full title: Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris

Authors: Hubertus Hohenblum; Brigitte Gasser; Michael Maurer; N. Borth; D. Mattanovich

Year: 2004

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.10904

Paper Genome‐scale metabolic model of Pichia pastoris with native and humanized glycosylation of recombinant proteins

Irani, Zahra Azimzadeh; Kerkhoven, Eduard J.; Shojaosadati, Seyed Abbas et al. · 2015 · Biotechnology and Bioengineering · DOI

Full title: Genome‐scale metabolic model of Pichia pastoris with native and humanized glycosylation of recombinant proteins

Authors: Irani, Zahra Azimzadeh; Kerkhoven, Eduard J.; Shojaosadati, Seyed Abbas; Nielsen, Jens

Year: 2015

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.25863

Paper Biosensor‐assisted engineering of a high‐yield Pichia pastoris cell‐free protein synthesis platform

Aw, Rochelle; Polizzi, Karen M. · 2018 · Biotechnology and Bioengineering · DOI

Full title: Biosensor‐assisted engineering of a high‐yield Pichia pastoris cell‐free protein synthesis platform

Authors: Aw, Rochelle; Polizzi, Karen M.

Year: 2018

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.26901

Paper In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response

C. Rebnegger; A. Graf; Minoska Valli et al. · 2013 · Biotechnology Journal · DOI

Full title: In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response

Authors: C. Rebnegger; A. Graf; Minoska Valli; Matthias G. Steiger; Brigitte Gasser; Michael Maurer; D. Mattanovich

Year: 2013

Venue: Biotechnology Journal

DOI: https://doi.org/10.1002/biot.201300334

Paper Process development for the continuous production of heterologous proteins by the industrial yeast, Komagataella phaffii

Cankorur‐Cetinkaya, Ayca; Narraidoo, Nathalie; Kasavi, Ceyda et al. · 2018 · Biotechnology and Bioengineering · DOI

Full title: Process development for the continuous production of heterologous proteins by the industrial yeast, Komagataella phaffii

Authors: Cankorur‐Cetinkaya, Ayca; Narraidoo, Nathalie; Kasavi, Ceyda; Slater, Nigel K.H.; Archer, David B.; Oliver, Stephen G.

Year: 2018

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.26846

Paper Metabolic modeling to identify engineering targets for Komagataella phaffii: The effect of biomass composition on gene target identification

Cankorur‐Cetinkaya, Ayca; Dikicioǧlu, Duygu; Oliver, Stephen G. · 2017 · Biotechnology and Bioengineering · DOI

Full title: Metabolic modeling to identify engineering targets for Komagataella phaffii: The effect of biomass composition on gene target identification

Authors: Cankorur‐Cetinkaya, Ayca; Dikicioǧlu, Duygu; Oliver, Stephen G.

Year: 2017

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.26380

Paper Challenging the workhorse: Comparative analysis of eukaryotic micro‐organisms for expressing monoclonal antibodies

Jiang, Hanxiao; Horwitz, Andrew A.; Wright, Chapman et al. · 2019 · Biotechnology and Bioengineering · DOI

Full title: Challenging the workhorse: Comparative analysis of eukaryotic micro‐organisms for expressing monoclonal antibodies

Authors: Jiang, Hanxiao; Horwitz, Andrew A.; Wright, Chapman; Tai, Anna; Znameroski, Elizabeth A.; Tsegaye, Yoseph; Warbington, Hailley; Bower, Benjamin S.; Alves, Christina; Co, Carl; Jonnalagadda, Kanvasri N.; Platt, Darren; Walter, Jessica M.; Natarajan, Venkatesh; Ubersax, Jeffrey A.; Cherry, Joel R.; Love, J. Christopher

Year: 2019

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.26951

Paper Expression of recombinant vitellogenin in the yeast Pichia pastoris

J. Ding; E. H. Lim; H.F. Li et al. · 2004 · Biotechnology and Bioengineering · DOI

Full title: Expression of recombinant vitellogenin in the yeast Pichia pastoris

Authors: J. Ding; E. H. Lim; H.F. Li; J. K. Kumar; S.L. Lee; T. Lam

Year: 2004

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.10800

Paper Engineering a versatile yeast platform for sesquiterpene production from glucose or methanol

Linhui Gao; Kun Zhang; Yiwei Shen et al. · 2024 · Biotechnology Journal · DOI

Full title: Engineering a versatile yeast platform for sesquiterpene production from glucose or methanol

Authors: Linhui Gao; Kun Zhang; Yiwei Shen; Peng Cai; Yongjin J. Zhou

Year: 2024

Venue: Biotechnology Journal

DOI: https://doi.org/10.1002/biot.202400261

Paper Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose-regulated GTH1 promoter of Pichia pastoris.

Prielhofer, Roland; Reichinger, Michaela; Wagner, Nina et al. · 2018 · Biotechnology and bioengineering · DOI

Full title: Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose-regulated GTH1 promoter of Pichia pastoris.

Authors: Prielhofer, Roland; Reichinger, Michaela; Wagner, Nina; Claes, Katrien; Kiziak, Christoph; Gasser, Brigitte; Mattanovich, Diethard

Year: 2018

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.26800

Paper Enhancement of protein secretion in Pichia pastoris by overexpression of protein disulfide isomerase.

Inan, Mehmet; Aryasomayajula, Dinesh; Sinha, Jayanta et al. · 2006 · Biotechnology and bioengineering · DOI

Full title: Enhancement of protein secretion in Pichia pastoris by overexpression of protein disulfide isomerase.

Authors: Inan, Mehmet; Aryasomayajula, Dinesh; Sinha, Jayanta; Meagher, Michael M

Year: 2006

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.20762

Paper Methanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors

T. Vogl; Lukas Sturmberger; P. Fauland et al. · 2018 · Biotechnology and Bioengineering · DOI

Full title: Methanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors

Authors: T. Vogl; Lukas Sturmberger; P. Fauland; P. Hyden; J. E. Fischer; Christian Schmid; G. Thallinger; M. Geier; A. Glieder

Year: 2018

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.26529

Paper Hypersecretory production of glucose oxidase in Pichia pastoris through combinatorial engineering of protein properties, synthesis, and secretion

Huzhi Zhou; Wenyu Zhang; Jiangchao Qian · 2023 · Biotechnology and Bioengineering · DOI

Full title: Hypersecretory production of glucose oxidase in Pichia pastoris through combinatorial engineering of protein properties, synthesis, and secretion

Authors: Huzhi Zhou; Wenyu Zhang; Jiangchao Qian

Year: 2023

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.28600

Paper Recombinant horseradish peroxidase variants for targeted cancer treatment

Bonifert, Günther; Folkes, Lisa K.; Gmeiner, Christoph et al. · 2016 · Cancer Medicine · DOI

Full title: Recombinant horseradish peroxidase variants for targeted cancer treatment

Authors: Bonifert, Günther; Folkes, Lisa K.; Gmeiner, Christoph; Dachs, Gabi U.; Spadiut, Oliver

Year: 2016

Venue: Cancer Medicine

DOI: https://doi.org/10.1002/cam4.668

Paper Characterization of methanol utilization negative Pichia pastoris for secreted protein production: New cultivation strategies for current and future applications

Zavec, Domen; Gasser, Brigitte; Mattanovich, Diethard · 2020 · Biotechnology and Bioengineering · DOI

Full title: Characterization of methanol utilization negative Pichia pastoris for secreted protein production: New cultivation strategies for current and future applications

Authors: Zavec, Domen; Gasser, Brigitte; Mattanovich, Diethard

Year: 2020

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.27303

Paper Economic analysis of royalactin production under uncertainty: Evaluating the effect of parameter optimization

Torres‐Acosta, Mario A.; Aguilar‐Yáñez, José Manuel; Rito‐Palomares, Marco et al. · 2015 · Biotechnology Progress · DOI

Full title: Economic analysis of royalactin production under uncertainty: Evaluating the effect of parameter optimization

Authors: Torres‐Acosta, Mario A.; Aguilar‐Yáñez, José Manuel; Rito‐Palomares, Marco; Titchener‐Hooker, Nigel J.

Year: 2015

Venue: Biotechnology Progress

DOI: https://doi.org/10.1002/btpr.2073

Paper Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain

Brady, Joseph R.; Whittaker, Charles A.; Tan, Melody C. et al. · 2019 · Biotechnology and Bioengineering · DOI

Full title: Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain

Authors: Brady, Joseph R.; Whittaker, Charles A.; Tan, Melody C.; Kristensen, D. Lee; Ma, Duanduan; Dalvie, Neil C.; Love, Kerry R.; Love, J. Christopher

Year: 2019

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.27209

Paper Effect of gene copy number and chaperone coexpression on recombinant hydrophobin HFBI biosurfactant production in Pichia pastoris.

Sallada, Nathanael D; Harkins, Lauren E; Berger, Bryan W · 2019 · Biotechnology and bioengineering · DOI

Full title: Effect of gene copy number and chaperone coexpression on recombinant hydrophobin HFBI biosurfactant production in Pichia pastoris.

Authors: Sallada, Nathanael D; Harkins, Lauren E; Berger, Bryan W

Year: 2019

Venue: Biotechnology and bioengineering

DOI: https://doi.org/10.1002/bit.26982

Paper Genetic engineering approaches to improve posttranslational modification of biopharmaceuticals in different production platforms

Amann, Thomas; Schmieder, Valerie; Kildegaard, Helene Faustrup et al. · 2019 · Biotechnology and Bioengineering · DOI

Full title: Genetic engineering approaches to improve posttranslational modification of biopharmaceuticals in different production platforms

Authors: Amann, Thomas; Schmieder, Valerie; Kildegaard, Helene Faustrup; Borth, Nicole; Andersen, Mikael Rørdam

Year: 2019

Venue: Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.27101

Paper Multiple Strategies for Recombinant Expression of Human Chymotrypsinogen C in

Yi Liu, Yu Xie, Xingyu Shen, Shihui Wang, Yi Xiong, Guimin Zhang · 2025 · Food Bioengineering · DOI

Full title: Multiple Strategies for Recombinant Expression of Human Chymotrypsinogen C in

Authors: Yi Liu, Yu Xie, Xingyu Shen, Shihui Wang, Yi Xiong, Guimin Zhang

Year: 2025

Venue: Food Bioengineering

DOI: https://doi.org/10.1002/fbe2.70034

Paper Recent developments on heterologous production of cellulases and xylanases using the Pichia pastoris expression system

Hayat Ullah; Meysam Madadi; M. Asadollahi et al. · 2023 · Journal of Chemical Technology & Biotechnology · DOI

Full title: Recent developments on heterologous production of cellulases and xylanases using the Pichia pastoris expression system

Authors: Hayat Ullah; Meysam Madadi; M. Asadollahi; Yun Hu; S. Dou; Junshu Yan; H. Huan; F. Sun

Year: 2023

Venue: Journal of Chemical Technology & Biotechnology

DOI: https://doi.org/10.1002/jctb.7570

Paper Evolving trends in mAb production processes

Shukla, Abhinav; Wolfe, Leslie S.; Mostafa, Sigma S. et al. · 2017 · Bioengineering & Translational Medicine · DOI

Full title: Evolving trends in mAb production processes

Authors: Shukla, Abhinav; Wolfe, Leslie S.; Mostafa, Sigma S.; Norman, Carnley

Year: 2017

Venue: Bioengineering & Translational Medicine

DOI: https://doi.org/10.1002/btm2.10061

Paper Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production

Rachel Daly; M. Hearn · 2005 · Journal of Molecular Recognition · DOI

Full title: Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production

Authors: Rachel Daly; M. Hearn

Year: 2005

Venue: Journal of Molecular Recognition

DOI: https://doi.org/10.1002/jmr.687

Paper Heterologous protein production using the Pichia pastoris expression system

Sue Macauley-Patrick; Mariana L. Fazenda; B. McNeil et al. · 2005 · Yeast · DOI

Full title: Heterologous protein production using the Pichia pastoris expression system

Authors: Sue Macauley-Patrick; Mariana L. Fazenda; B. McNeil; L. Harvey

Year: 2005

Venue: Yeast

DOI: https://doi.org/10.1002/yea.1208

Paper Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

Karbalaei, Mohsen; Rezaee, Seyed Abdolrahim; Farsiani, Hadi · 2020 · Journal of Cellular Physiology · DOI

Full title: Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

Authors: Karbalaei, Mohsen; Rezaee, Seyed Abdolrahim; Farsiani, Hadi

Year: 2020

Venue: Journal of Cellular Physiology

DOI: https://doi.org/10.1002/jcp.29583

Paper Heterologous Protein Expression in Pichia pastoris: Latest Research Progress and Applications

Veeresh Juturu; J. Wu · 2018 · ChemBioChem · DOI

Full title: Heterologous Protein Expression in Pichia pastoris: Latest Research Progress and Applications

Authors: Veeresh Juturu; J. Wu

Year: 2018

Venue: ChemBioChem

DOI: https://doi.org/10.1002/cbic.201700460

Paper Crystal structure of a triacylglycerol lipase from Penicillium expansum at 1.3 Å determined by sulfur SAD

Bian, Chuanbing; Yuan, Cai; Chen, Liqing et al. · 2009 · Proteins Structure Function and Bioinformatics · DOI

Full title: Crystal structure of a triacylglycerol lipase from Penicillium expansum at 1.3 Å determined by sulfur SAD

Authors: Bian, Chuanbing; Yuan, Cai; Chen, Liqing; Meehan, Edward J.; Jiang, Longguang; Huang, Zixiang; Lin, Lin; Huang, Mingdong

Year: 2009

Venue: Proteins Structure Function and Bioinformatics

DOI: https://doi.org/10.1002/prot.22676

Paper ¹³C-based metabolic flux analysis of recombinant Pichia pastoris.

Ferrer, Pau; Albiol, Joan · 2014 · Methods in molecular biology (Clifton, N.J.) · DOI

Full title: ¹³C-based metabolic flux analysis of recombinant Pichia pastoris.

Authors: Ferrer, Pau; Albiol, Joan

Year: 2014

Venue: Methods in molecular biology (Clifton, N.J.)

DOI: https://doi.org/10.1007/978-1-4939-1170-7_17

Paper High-Throughput Optimization of Recombinant Protein Production in Microfluidic Gel Beads.

Napiorkowska, Marta; Pestalozzi, Luzius; Panke, Sven et al. · 2021 · Small (Weinheim an der Bergstrasse, Germany) · DOI

Full title: High-Throughput Optimization of Recombinant Protein Production in Microfluidic Gel Beads.

Authors: Napiorkowska, Marta; Pestalozzi, Luzius; Panke, Sven; Held, Martin; Schmitt, Steven

Year: 2021

Venue: Small (Weinheim an der Bergstrasse, Germany)

DOI: https://doi.org/10.1002/smll.202005523

Paper Highly efficient expression of Rasamsonia emersonii lipase in Pichia pastoris: Characterization and gastrointestinal simulated digestion in vitro.

Buqing Wang; Yasen Wang; Xiaoman Zhou et al. · 2024 · The Journal of the Science of Food and Agriculture · DOI

Full title: Highly efficient expression of Rasamsonia emersonii lipase in Pichia pastoris: Characterization and gastrointestinal simulated digestion in vitro.

Authors: Buqing Wang; Yasen Wang; Xiaoman Zhou; Xiao-Dong Gao; Morihisa Fujita; Zijie Li

Year: 2024

Venue: The Journal of the Science of Food and Agriculture

DOI: https://doi.org/10.1002/jsfa.13390

Paper Pichia pastoris protease‐deficient and auxotrophic strains generated by a novel, user‐friendly vector toolbox for gene deletion

Ahmad, Mudassar; Winkler, Christine; Kolmbauer, Markus et al. · 2019 · Yeast · DOI

Full title: Pichia pastoris protease‐deficient and auxotrophic strains generated by a novel, user‐friendly vector toolbox for gene deletion

Authors: Ahmad, Mudassar; Winkler, Christine; Kolmbauer, Markus; Pichler, Harald; Schwab, Helmut; Emmerstorfer‐Augustin, Anita

Year: 2019

Venue: Yeast

DOI: https://doi.org/10.1002/yea.3426

Paper Engineering of Promoters for Gene Expression in Pichia pastoris.

T. Vogl · 2022 · Methods in molecular biology · DOI

Full title: Engineering of Promoters for Gene Expression in Pichia pastoris.

Authors: T. Vogl

Year: 2022

Venue: Methods in molecular biology

DOI: https://doi.org/10.1007/978-1-0716-2399-2_10

Paper Hygromycin-resistance vectors for gene expression in Pichia pastoris

Yang, Junjie; Nie, Lei; Chen, Biao et al. · 2014 · Yeast · DOI

Full title: Hygromycin-resistance vectors for gene expression in Pichia pastoris

Authors: Yang, Junjie; Nie, Lei; Chen, Biao; Liu, Yingmiao; Kong, Yimeng; Wang, Haibin; Diao, Liuyang

Year: 2014

Venue: Yeast

DOI: https://doi.org/10.1002/yea.3001

Paper CRISPR/Cas9-Mediated Homology-Directed Genome Editing in Pichia pastoris.

T. Gassler; Lina Heistinger; D. Mattanovich et al. · 2019 · Methods in molecular biology · DOI

Full title: CRISPR/Cas9-Mediated Homology-Directed Genome Editing in Pichia pastoris.

Authors: T. Gassler; Lina Heistinger; D. Mattanovich; Brigitte Gasser; R. Prielhofer

Year: 2019

Venue: Methods in molecular biology

DOI: https://doi.org/10.1007/978-1-4939-9024-5_9

Paper Decrease of hirudin degradation by deleting the KEX1 gene in recombinant Pichia pastoris

Z. Ni; Xiangshan Zhou; Xueqian Sun et al. · 2008 · Yeast · DOI

Full title: Decrease of hirudin degradation by deleting the KEX1 gene in recombinant Pichia pastoris

Authors: Z. Ni; Xiangshan Zhou; Xueqian Sun; Ya Wang; Yuanxing Zhang

Year: 2008

Venue: Yeast

DOI: https://doi.org/10.1002/yea.1542

Paper Promoters in Pichia pastoris: A Toolbox for Fine-Tuned Gene Expression.

Arjmand, Sareh · 2024 · Methods in molecular biology (Clifton, N.J.) · DOI

Full title: Promoters in Pichia pastoris: A Toolbox for Fine-Tuned Gene Expression.

Authors: Arjmand, Sareh

Year: 2024

Venue: Methods in molecular biology (Clifton, N.J.)

DOI: https://doi.org/10.1007/978-1-0716-4063-0_11

Paper Effects of co-overexpression of secretion helper factors on the secretion of a HSA fusion protein (IL2-HSA) in pichia pastoris.

Guan, Bo; Chen, Fengxiang; Su, Shuai et al. · 2016 · Yeast (Chichester, England) · DOI

Full title: Effects of co-overexpression of secretion helper factors on the secretion of a HSA fusion protein (IL2-HSA) in pichia pastoris.

Authors: Guan, Bo; Chen, Fengxiang; Su, Shuai; Duan, Zuoying; Chen, Yun; Li, Huazhong; Jin, Jian

Year: 2016

Venue: Yeast (Chichester, England)

DOI: https://doi.org/10.1002/yea.3183

Paper Biosynthetic production of type II fish antifreeze protein: fermentation by Pichia pastoris

M. Loewen; X. Liu; P. Davies et al. · 1997 · Applied Microbiology and Biotechnology · DOI

Full title: Biosynthetic production of type II fish antifreeze protein: fermentation by Pichia pastoris

Authors: M. Loewen; X. Liu; P. Davies; A. Daugulis

Year: 1997

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s002530051083

Paper Production of Functionalized Single-Chain Fv Antibody Fragments Binding to the ED-B Domain of the B-isoform of Fibronectin in Pichia pastoris

Marty, Cornelia; Scheidegger, Patrick; Ballmer‐Hofer, Kurt et al. · 2001 · Protein Expression and Purification · DOI

Full title: Production of Functionalized Single-Chain Fv Antibody Fragments Binding to the ED-B Domain of the B-isoform of Fibronectin in Pichia pastoris

Authors: Marty, Cornelia; Scheidegger, Patrick; Ballmer‐Hofer, Kurt; Klemenz, R; Schwendener, Reto A.

Year: 2001

Venue: Protein Expression and Purification

DOI: https://doi.org/10.1006/prep.2000.1362

Paper Effect of cooperation of chaperones and gene dosage on the expression of porcine PGLYRP-1 in Pichia pastoris

Jun Yang; Zhipeng Lu; Jiawei Chen et al. · 2016 · Applied Microbiology and Biotechnology · DOI

Full title: Effect of cooperation of chaperones and gene dosage on the expression of porcine PGLYRP-1 in Pichia pastoris

Authors: Jun Yang; Zhipeng Lu; Jiawei Chen; Pinpin Chu; Qingmei Cheng; Jie Liu; Fei-ping Ming; Chaoyuan Huang; Anji Xiao; Haiming Cai; Linghua Zhang

Year: 2016

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-016-7372-4

Paper Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris

Leonardo M. Damasceno; K. Anderson; G. Ritter et al. · 2007 · Applied Microbiology and Biotechnology · DOI

Full title: Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris

Authors: Leonardo M. Damasceno; K. Anderson; G. Ritter; J. Cregg; L. Old; C. Batt

Year: 2007

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-006-0652-7

Paper High-level constitutive expression of leech hyaluronidase with combined strategies in recombinant Pichia pastoris

Hao Huang; Qixing Liang; Yang Wang et al. · 2020 · Applied Microbiology and Biotechnology · DOI

Full title: High-level constitutive expression of leech hyaluronidase with combined strategies in recombinant Pichia pastoris

Authors: Hao Huang; Qixing Liang; Yang Wang; Jian Chen; Zhen Kang

Year: 2020

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-019-10282-7

Paper Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications

Radin Shafierul Radin Yahaya; Yahaya M. Normi; L. Phang et al. · 2021 · Applied Microbiology and Biotechnology · DOI

Full title: Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications

Authors: Radin Shafierul Radin Yahaya; Yahaya M. Normi; L. Phang; S. Ahmad; J. Abdullah; S. Sabri

Year: 2021

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-021-11321-y

Paper A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris

Xihao Liao; Lu Li; Aysha Jameel et al. · 2021 · Applied Microbiology and Biotechnology · DOI

Full title: A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris

Authors: Xihao Liao; Lu Li; Aysha Jameel; X. Xing; Chong Zhang

Year: 2021

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-021-11688-y

Paper Engineering a Pichia pastoris nitrilase whole cell catalyst through the increased nitrilase gene copy number and co-expressing of ER oxidoreductin 1

Qi Shen; Zhuang Yu; P. Lv et al. · 2020 · Applied Microbiology and Biotechnology · DOI

Full title: Engineering a Pichia pastoris nitrilase whole cell catalyst through the increased nitrilase gene copy number and co-expressing of ER oxidoreductin 1

Authors: Qi Shen; Zhuang Yu; P. Lv; Qian Li; Shuping Zou; Neng Xiong; Zhi-qiang Liu; Ya-ping Xue; Yuguo Zheng

Year: 2020

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-020-10422-4

Paper Pathway engineering facilitates efficient protein expression in Pichia pastoris

Chao Liu; Jin-Song Gong; Chang Su et al. · 2022 · Applied Microbiology and Biotechnology · DOI

Full title: Pathway engineering facilitates efficient protein expression in Pichia pastoris

Authors: Chao Liu; Jin-Song Gong; Chang Su; Hui Li; Heng Li; Z. Rao; Zhenghong Xu; Jin-song Shi

Year: 2022

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-022-12139-y

Paper Design of a novel switchable antibody display system in Pichia pastoris

Dominic Gätjen; Florian Tomszak; Johann-Christoph Dettmann et al. · 2022 · Applied Microbiology and Biotechnology · DOI

Full title: Design of a novel switchable antibody display system in Pichia pastoris

Authors: Dominic Gätjen; Florian Tomszak; Johann-Christoph Dettmann; Miriam Droste; V. Nölle; M. Wieczorek

Year: 2022

Venue: Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-022-12108-5

Paper Lignocellulose degrading extremozymes produced by Pichia pastoris: current status and future prospects

Burcu Gündüz Ergün; P. Çalık · 2015 · Bioprocess and biosystems engineering (Print) · DOI

Full title: Lignocellulose degrading extremozymes produced by Pichia pastoris: current status and future prospects

Authors: Burcu Gündüz Ergün; P. Çalık

Year: 2015

Venue: Bioprocess and biosystems engineering (Print)

DOI: https://doi.org/10.1007/s00449-015-1476-6

Paper [Delivery of recombinant enhanced green fluorescent protein to Pichia pastoris cell wall directed by a mammalian nonclassical secretion signal peptide].

Yufeng Qin; Yaosheng Chen; Zhiguo Liu et al. · 2016 · Sheng wu gong cheng xue bao = Chinese journal of biotechnology · DOI

Full title: [Delivery of recombinant enhanced green fluorescent protein to Pichia pastoris cell wall directed by a mammalian nonclassical secretion signal peptide].

Authors: Yufeng Qin; Yaosheng Chen; Zhiguo Liu; Ying Zhang; Hailong Liu; Zuyong He

Year: 2016

Venue: Sheng wu gong cheng xue bao = Chinese journal of biotechnology

DOI: https://doi.org/10.13345/j.cjb.160007

Paper Biologically active recombinant carp LH as a spawning-inducing agent for carp

Aizen, Joseph; Hollander-Cohen, Lian; Shpilman, Michal et al. · 2016 · Journal of Endocrinology · DOI

Full title: Biologically active recombinant carp LH as a spawning-inducing agent for carp

Authors: Aizen, Joseph; Hollander-Cohen, Lian; Shpilman, Michal; Levavi‐Sivan, Berta

Year: 2016

Venue: Journal of Endocrinology

DOI: https://doi.org/10.1530/joe-16-0435

Paper Relieving the first bottleneck in the drug discovery pipeline: using array technologies to rationalize membrane protein production

Bonander, Nicklas; Bill, Roslyn M. · 2009 · Expert Review of Proteomics · DOI

Full title: Relieving the first bottleneck in the drug discovery pipeline: using array technologies to rationalize membrane protein production

Authors: Bonander, Nicklas; Bill, Roslyn M.

Year: 2009

Venue: Expert Review of Proteomics

DOI: https://doi.org/10.1586/epr.09.65

Paper Recombinant gene expression : reviews and protocols

P. Balbás; A. Lorence · 2012 · DOI

Full title: Recombinant gene expression : reviews and protocols

Authors: P. Balbás; A. Lorence

Year: 2012

DOI: https://doi.org/10.1385/1592597742

Paper Diversity and occurrence of methylotrophic yeasts used in genetic engineering

A. S. Rozanov; E. G. Pershina; N. V. Bogacheva et al. · 2020 · Vavilovskii zhurnal genetiki i selektsii · DOI

Full title: Diversity and occurrence of methylotrophic yeasts used in genetic engineering

Authors: A. S. Rozanov; E. G. Pershina; N. V. Bogacheva; V. Shlyakhtun; A. Sychev; S. Peltek

Year: 2020

Venue: Vavilovskii zhurnal genetiki i selektsii

DOI: https://doi.org/10.18699/vj20.602

Paper Expression and secretion of a biologically active glycoprotein hormone, ovine follicle stimulating hormone, by Pichia pastoris

Fidler, AE; Lun, S.; Young, Wayne et al. · 1998 · Journal of Molecular Endocrinology · DOI

Full title: Expression and secretion of a biologically active glycoprotein hormone, ovine follicle stimulating hormone, by Pichia pastoris

Authors: Fidler, AE; Lun, S.; Young, Wayne; McNatty, KP

Year: 1998

Venue: Journal of Molecular Endocrinology

DOI: https://doi.org/10.1677/jme.0.0210327

Paper Expression, purification and characterization of a phyA m -phyCs fusion phytase

Zou, Likou; Wang, Hongning; Pan, Xin et al. · 2008 · Journal of Zhejiang University SCIENCE B · DOI

Full title: Expression, purification and characterization of a phyA m -phyCs fusion phytase

Authors: Zou, Likou; Wang, Hongning; Pan, Xin; Tian, Guo‐Bao; Xie, Zi-wen; Wu, Qi; Chen, Hui; Xie, Tao; Zhi-rong, Yang

Year: 2008

Venue: Journal of Zhejiang University SCIENCE B

DOI: https://doi.org/10.1631/jzus.b0720006

Paper Production of biologically active tethered ovine FSHbetaalpha by the methylotrophic yeast Pichia pastoris

Fidler, AE; Lin, Jian‐Sheng; Lun, S. et al. · 2003 · Journal of Molecular Endocrinology · DOI

Full title: Production of biologically active tethered ovine FSHbetaalpha by the methylotrophic yeast Pichia pastoris

Authors: Fidler, AE; Lin, Jian‐Sheng; Lun, S.; Chie, W Ng; Western, Andrea; Stent, Vicki; McNatty, KP

Year: 2003

Venue: Journal of Molecular Endocrinology

DOI: https://doi.org/10.1677/jme.0.0300213

Paper Recombinant strains producing thermostable lipase from Thermomyces lanuginosus and their use in heterogeneous biocatalysis, including in the processes of low-temperature synthesis of esters

Пыхтина, М. Б.; Перминова, Л. В.; Коваленко, Г. А. · 2021 · Biotekhnologiya · DOI

Full title: Recombinant strains producing thermostable lipase from Thermomyces lanuginosus and their use in heterogeneous biocatalysis, including in the processes of low-temperature synthesis of esters

Authors: Пыхтина, М. Б.; Перминова, Л. В.; Коваленко, Г. А.

Year: 2021

Venue: Biotekhnologiya

DOI: https://doi.org/10.21519/0234-2758-2021-37-5-5-19

Paper Increased expression of recombinant chitosanase by co-expression of Hac1p in the yeast Pichia pastoris.

Minghai Han; Weixian Wang; Xun Gong et al. · 2021 · Protein Peptide Letters · DOI

Full title: Increased expression of recombinant chitosanase by co-expression of Hac1p in the yeast Pichia pastoris.

Authors: Minghai Han; Weixian Wang; Xun Gong; Jianli Zhou; Cunbin Xu; Yinfeng Li

Year: 2021

Venue: Protein Peptide Letters

DOI: https://doi.org/10.2174/0929866528666211105111155

Paper New Developments in Pichia pastoris Expression System, Review and Update.

Roghayyeh Baghban; S. Farajnia; Y. Ghasemi et al. · 2018 · Current Pharmaceutical Biotechnology · DOI

Full title: New Developments in Pichia pastoris Expression System, Review and Update.

Authors: Roghayyeh Baghban; S. Farajnia; Y. Ghasemi; M. Mortazavi; N. Zarghami; N. Samadi

Year: 2018

Venue: Current Pharmaceutical Biotechnology

DOI: https://doi.org/10.2174/1389201019666180718093037

Paper Production systems for recombinant antibodies

Schirrmann, Thomas · 2008 · Frontiers in bioscience · DOI

Full title: Production systems for recombinant antibodies

Authors: Schirrmann, Thomas

Year: 2008

Venue: Frontiers in bioscience

DOI: https://doi.org/10.2741/3024

Paper Gene and process level modulation to overcome the bottlenecks of recombinant proteins expression in Pichia pastoris.

Ashish A. Prabhu; Bibari Boro; B. Bharali et al. · 2018 · Current Pharmaceutical Biotechnology · DOI

Full title: Gene and process level modulation to overcome the bottlenecks of recombinant proteins expression in Pichia pastoris.

Authors: Ashish A. Prabhu; Bibari Boro; B. Bharali; S. Chakraborty; V. V. Dasu

Year: 2018

Venue: Current Pharmaceutical Biotechnology

DOI: https://doi.org/10.2174/1389201019666180329112827

Paper Production of enzymes by biotechnological methods: A review

A. Dub; Iuliia Plaskonis; Oksana Barna et al. · 2025 · The Ukrainian Scientific Medical Youth Journal · DOI

Full title: Production of enzymes by biotechnological methods: A review

Authors: A. Dub; Iuliia Plaskonis; Oksana Barna; Halyna Kozyr; I. Stechyshyn; Mariana Vasenda

Year: 2025

Venue: The Ukrainian Scientific Medical Youth Journal

DOI: https://doi.org/10.32345/usmyj.3(157).2025.161-167

Paper [Enzymatic activity of recombinant metallopeptidase for further using in meat industry].

Makhova, A.A.; Minaev, M. Yu.; Kulikovsky, A.V. et al. · 2020 · PubMed · DOI

Full title: [Enzymatic activity of recombinant metallopeptidase for further using in meat industry].

Authors: Makhova, A.A.; Minaev, M. Yu.; Kulikovsky, A.V.; Вострикова, Н. Л.

Year: 2020

Venue: PubMed

DOI: https://doi.org/10.24411/0042-8833-2019-10047

Paper Extracellular Expression of Recombinant Kex2 Protease in Pichia pastoris BG10

· 2022 · Biointerface Research in Applied Chemistry · DOI

Full title: Extracellular Expression of Recombinant Kex2 Protease in Pichia pastoris BG10

Year: 2022

Venue: Biointerface Research in Applied Chemistry

DOI: https://doi.org/10.33263/briac134.360

Paper Expression of MTHase Gene in Pichia pastoris and Its Genetic Stability

Li, Dehua · 2010

Full title: Expression of MTHase Gene in Pichia pastoris and Its Genetic Stability

Authors: Li, Dehua

Year: 2010

Paper Engineering the Pattern of Protein Glycosylation Modulates the Thermostability of a GH11 Xylanase

Fonseca-Maldonado, Raquel; Vieira, Davi Serradella; Alponti, Juliana Sanchez et al. · 2013 · Journal of Biological Chemistry · DOI

Full title: Engineering the Pattern of Protein Glycosylation Modulates the Thermostability of a GH11 Xylanase

Authors: Fonseca-Maldonado, Raquel; Vieira, Davi Serradella; Alponti, Juliana Sanchez; Bonneil, Éric; Thibault, Pierre; Ward, Richard J.

Year: 2013

Venue: Journal of Biological Chemistry

DOI: https://doi.org/10.1074/jbc.m113.485953

Paper Structural Analysis of a Glycoside Hydrolase Family 11 Xylanase from Neocallimastix patriciarum

Cheng, Ya‐Shan; Chen, Chun‐Chi; Huang, Chun‐Hsiang et al. · 2014 · Journal of Biological Chemistry · DOI

Full title: Structural Analysis of a Glycoside Hydrolase Family 11 Xylanase from Neocallimastix patriciarum

Authors: Cheng, Ya‐Shan; Chen, Chun‐Chi; Huang, Chun‐Hsiang; Ko, Tzu‐Ping; Luo, Wen-Hua; Huang, Jian‐Wen; Liu, Je‐Ruei; Guo, Rey‐Ting

Year: 2014

Venue: Journal of Biological Chemistry

DOI: https://doi.org/10.1074/jbc.m114.550905

Paper The importance of connections between the cell wall integrity pathway and the unfolded protein response in filamentous fungi

Malavazi, Iran; Goldman, Gustavo H.; Brown, Neil · 2014 · Briefings in Functional Genomics · DOI

Full title: The importance of connections between the cell wall integrity pathway and the unfolded protein response in filamentous fungi

Authors: Malavazi, Iran; Goldman, Gustavo H.; Brown, Neil

Year: 2014

Venue: Briefings in Functional Genomics

DOI: https://doi.org/10.1093/bfgp/elu027

Paper Specific Characterization of Substrate and Inhibitor Binding Sites of a Glycosyl Hydrolase Family 11 Xylanase fromAspergillus niger

Tahir, Tariq A.; Berrin, Jean‐Guy; Flatman, Ruth H. et al. · 2002 · Journal of Biological Chemistry · DOI

Full title: Specific Characterization of Substrate and Inhibitor Binding Sites of a Glycosyl Hydrolase Family 11 Xylanase fromAspergillus niger

Authors: Tahir, Tariq A.; Berrin, Jean‐Guy; Flatman, Ruth H.; Roussel, Alain; Roepstorff, Peter; Williamson, Gary; Juge, Nathalie

Year: 2002

Venue: Journal of Biological Chemistry

DOI: https://doi.org/10.1074/jbc.m205657200

Paper A Fast-acting, Modular-structured Staphylokinase Fusion with Kringle-1 from Human Plasminogen as the Fibrin-targeting Domain Offers Improved Clot Lysis Efficacy

Wu, Sau‐Ching; Castellino, Francis; Wong, Sui‐Lam · 2003 · Journal of Biological Chemistry · DOI

Full title: A Fast-acting, Modular-structured Staphylokinase Fusion with Kringle-1 from Human Plasminogen as the Fibrin-targeting Domain Offers Improved Clot Lysis Efficacy

Authors: Wu, Sau‐Ching; Castellino, Francis; Wong, Sui‐Lam

Year: 2003

Venue: Journal of Biological Chemistry

DOI: https://doi.org/10.1074/jbc.m210919200

Paper Glycosylation Increases the Thermostability of Human Aquaporin 10 Protein

Öberg, Fredrik; Sjöhamn, Jennie; Fischer, Gerhard W. et al. · 2011 · Journal of Biological Chemistry · DOI

Full title: Glycosylation Increases the Thermostability of Human Aquaporin 10 Protein

Authors: Öberg, Fredrik; Sjöhamn, Jennie; Fischer, Gerhard W.; Moberg, Andreas; Pedersen, Anders; Neutze, Richard; Hedfalk, Kristina

Year: 2011

Venue: Journal of Biological Chemistry

DOI: https://doi.org/10.1074/jbc.m111.242677

Paper Efforts to make and apply humanized yeast

Laurent, Jon M.; Young, Jonathan H.; Kachroo, Aashiq H. et al. · 2015 · Briefings in Functional Genomics · DOI

Full title: Efforts to make and apply humanized yeast

Authors: Laurent, Jon M.; Young, Jonathan H.; Kachroo, Aashiq H.; Marcotte, Edward M.

Year: 2015

Venue: Briefings in Functional Genomics

DOI: https://doi.org/10.1093/bfgp/elv041

Paper Bioprospecting and biotechnological insights into sweet-tasting proteins by microbial hosts―a review

M. Bilal; Liyun Ji; Shuo Xu et al. · 2022 · Bioengineered · DOI

Full title: Bioprospecting and biotechnological insights into sweet-tasting proteins by microbial hosts―a review

Authors: M. Bilal; Liyun Ji; Shuo Xu; Yue Zhang; Hafiz M.N. Iqbal; Hairong Cheng

Year: 2022

Venue: Bioengineered

DOI: https://doi.org/10.1080/21655979.2022.2061147

Paper Towards a better understanding of the generation of fructan structure diversity in plants: molecular and functional characterization of a sucrose:fructan 6-fructosyltransferase (6-SFT) cDNA from perennial ryegrass (Lolium perenne)

Lasseur, Bertrand; Lothier, J.; Wiemken, Andres et al. · 2010 · Journal of Experimental Botany · DOI

Full title: Towards a better understanding of the generation of fructan structure diversity in plants: molecular and functional characterization of a sucrose:fructan 6-fructosyltransferase (6-SFT) cDNA from perennial ryegrass (Lolium perenne)

Authors: Lasseur, Bertrand; Lothier, J.; Wiemken, Andres; Laere, A. Van; Morvan‐Bertrand, Annette; Ende, Wim Van den; PRUD'HOMME, M. P.

Year: 2010

Venue: Journal of Experimental Botany

DOI: https://doi.org/10.1093/jxb/erq388

Paper Yeast synthetic biology for designed cell factories producing secretory recombinant proteins

Thak, Eun Jung; Yoo, Su Jin; Moon, Hye Yun et al. · 2020 · FEMS Yeast Research · DOI

Full title: Yeast synthetic biology for designed cell factories producing secretory recombinant proteins

Authors: Thak, Eun Jung; Yoo, Su Jin; Moon, Hye Yun; Kang, Hyun Ah

Year: 2020

Venue: FEMS Yeast Research

DOI: https://doi.org/10.1093/femsyr/foaa009

Paper Editorial for the thematic tssue on "Industrial Microbiology".

Colin R. Harwood; Sunghoon Park; Michael Sauer · 2018 · FEMS Microbiology Letters · DOI

Full title: Editorial for the thematic tssue on "Industrial Microbiology".

Authors: Colin R. Harwood; Sunghoon Park; Michael Sauer

Year: 2018

Venue: FEMS Microbiology Letters

DOI: https://doi.org/10.1093/femsle/fny275

Paper Production of lycopene by metabolically engineered Pichia pastoris

Xinying Zhang; Denggang Wang; Yehong Duan et al. · 2019 · Bioscience, biotechnology and biochemistry · DOI

Full title: Production of lycopene by metabolically engineered Pichia pastoris

Authors: Xinying Zhang; Denggang Wang; Yehong Duan; Xueyun Zheng; Ying-Wu Lin; Shuli Liang

Year: 2019

Venue: Bioscience, biotechnology and biochemistry

DOI: https://doi.org/10.1080/09168451.2019.1693250

Paper Recombinant protein production in Pichia pastoris: from transcriptionally redesigned strains to bioprocess optimization and metabolic modelling.

Ergün, Burcu Gündüz; Berrios, Julio; Binay, Barış et al. · 2021 · FEMS yeast research · DOI

Full title: Recombinant protein production in Pichia pastoris: from transcriptionally redesigned strains to bioprocess optimization and metabolic modelling.

Authors: Ergün, Burcu Gündüz; Berrios, Julio; Binay, Barış; Fickers, Patrick

Year: 2021

Venue: FEMS yeast research

DOI: https://doi.org/10.1093/femsyr/foab057

Paper Expression of chromogranin A-derived antifungal peptide CGA-N12 in Pichia pastoris

Xiaohua Li; Y. Fan; Qiong Lin et al. · 2020 · Bioengineered · DOI

Full title: Expression of chromogranin A-derived antifungal peptide CGA-N12 in Pichia pastoris

Authors: Xiaohua Li; Y. Fan; Qiong Lin; Jianxiong Luo; Yide Huang; Y. Bao; Liyu Xu

Year: 2020

Venue: Bioengineered

DOI: https://doi.org/10.1080/21655979.2020.1736237

Paper Improved production of recombinant Rhizomucor miehei lipase by coexpressing protein folding chaperones in Pichia pastoris, which triggered ER stress.

Huang, Jinjin; Zhao, Qingyi; Chen, Lingxiao et al. · 2020 · Bioengineered · DOI

Full title: Improved production of recombinant Rhizomucor miehei lipase by coexpressing protein folding chaperones in Pichia pastoris, which triggered ER stress.

Authors: Huang, Jinjin; Zhao, Qingyi; Chen, Lingxiao; Zhang, Chunmei; Bu, Wei; Zhang, Xin; Zhang, Kaini; Yang, Zhen

Year: 2020

Venue: Bioengineered

DOI: https://doi.org/10.1080/21655979.2020.1738127

Paper Multiplex genome editing of microorganisms using CRISPR-Cas

Adiego-Pérez, Belén; Randazzo, Paola; Daran, Jean‐Marc et al. · 2019 · FEMS Microbiology Letters · DOI

Full title: Multiplex genome editing of microorganisms using CRISPR-Cas

Authors: Adiego-Pérez, Belén; Randazzo, Paola; Daran, Jean‐Marc; Verwaal, René; Roubos, Johannes A.; Daran‐Lapujade, Pascale; Oost, John van der

Year: 2019

Venue: FEMS Microbiology Letters

DOI: https://doi.org/10.1093/femsle/fnz086

Paper What makes Komagataella phaffii non-conventional?

Ata, Özge; Ergün, Burcu Gündüz; Fickers, Patrick et al. · 2021 · FEMS Yeast Research · DOI

Full title: What makes Komagataella phaffii non-conventional?

Authors: Ata, Özge; Ergün, Burcu Gündüz; Fickers, Patrick; Heistinger, Lina; Mattanovich, Diethard; Rebnegger, Corinna; Gasser, Brigitte

Year: 2021

Venue: FEMS Yeast Research

DOI: https://doi.org/10.1093/femsyr/foab059

Paper Systems biotechnology for protein production in Pichia pastoris.

Zahrl, Richard J; Peña, David A; Mattanovich, Diethard et al. · 2017 · FEMS yeast research · DOI

Full title: Systems biotechnology for protein production in Pichia pastoris.

Authors: Zahrl, Richard J; Peña, David A; Mattanovich, Diethard; Gasser, Brigitte

Year: 2017

Venue: FEMS yeast research

DOI: https://doi.org/10.1093/femsyr/fox068

Paper A review of yeast: High cell-density culture, molecular mechanisms of stress response and tolerance during fermentation

Shen, Dongxu; He, Xiaoli; Weng, Peifang et al. · 2022 · FEMS Yeast Research · DOI

Full title: A review of yeast: High cell-density culture, molecular mechanisms of stress response and tolerance during fermentation

Authors: Shen, Dongxu; He, Xiaoli; Weng, Peifang; Liu, Yanan; Wu, Zufang

Year: 2022

Venue: FEMS Yeast Research

DOI: https://doi.org/10.1093/femsyr/foac050

Paper Yeast-based vaccines: New perspective in vaccine development and application

Kumar, Ravinder; Kumar, Piyush · 2019 · FEMS Yeast Research · DOI

Full title: Yeast-based vaccines: New perspective in vaccine development and application

Authors: Kumar, Ravinder; Kumar, Piyush

Year: 2019

Venue: FEMS Yeast Research

DOI: https://doi.org/10.1093/femsyr/foz007

Paper A yeast for all seasons – Is Pichia pastoris a suitable chassis organism for future bioproduction?

Gasser, Brigitte; Mattanovich, Diethard · 2018 · FEMS Microbiology Letters · DOI

Full title: A yeast for all seasons – Is Pichia pastoris a suitable chassis organism for future bioproduction?

Authors: Gasser, Brigitte; Mattanovich, Diethard

Year: 2018

Venue: FEMS Microbiology Letters

DOI: https://doi.org/10.1093/femsle/fny181

Paper CRISPR/Cas system for yeast genome engineering: advances and applications

Šťovíček, Vratislav; Holkenbrink, Carina; Borodina, Irina · 2017 · FEMS Yeast Research · DOI

Full title: CRISPR/Cas system for yeast genome engineering: advances and applications

Authors: Šťovíček, Vratislav; Holkenbrink, Carina; Borodina, Irina

Year: 2017

Venue: FEMS Yeast Research

DOI: https://doi.org/10.1093/femsyr/fox030

Paper Glyco-variant library of the versatile enzyme horseradish peroxidase

Simona Capone; Robert Pletzenauer; D. Maresch et al. · 2014 · Glycobiology · DOI

Full title: Glyco-variant library of the versatile enzyme horseradish peroxidase

Authors: Simona Capone; Robert Pletzenauer; D. Maresch; K. Metzger; F. Altmann; C. Herwig; O. Spadiut

Year: 2014

Venue: Glycobiology

DOI: https://doi.org/10.1093/glycob/cwu047

Paper Differences in enzymic properties of five recombinant xyloglucan endotransglucosylase/hydrolase (XTH) proteins of Arabidopsis thaliana

Maris, A.; Kaewthai, Nomchit; Eklöf, Jens et al. · 2010 · Journal of Experimental Botany · DOI

Full title: Differences in enzymic properties of five recombinant xyloglucan endotransglucosylase/hydrolase (XTH) proteins of Arabidopsis thaliana

Authors: Maris, A.; Kaewthai, Nomchit; Eklöf, Jens; Miller, Janice G.; Brumer, Harry; Fry, Stephen C.; Verbelen, Jean‐Pierre; Vissenberg, Kris

Year: 2010

Venue: Journal of Experimental Botany

DOI: https://doi.org/10.1093/jxb/erq263

Paper Engineering the expression system for Komagataella phaffii (Pichia pastoris): an attempt to develop a methanol-free expression system

S. Takagi; N. Tsutsumi; Yuji Terui et al. · 2019 · FEMS Yeast Research · DOI

Full title: Engineering the expression system for Komagataella phaffii (Pichia pastoris): an attempt to develop a methanol-free expression system

Authors: S. Takagi; N. Tsutsumi; Yuji Terui; Xiangyu Kong; H. Yurimoto; Y. Sakai

Year: 2019

Venue: FEMS Yeast Research

DOI: https://doi.org/10.1093/femsyr/foz059

Paper Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression

Y. Ito; G. Terai; M. Ishigami et al. · 2020 · Nucleic Acids Research · DOI

Full title: Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression

Authors: Y. Ito; G. Terai; M. Ishigami; Noriko Hashiba; Yasuyuki Nakamura; Takahiro Bamba; Ryota Kumokita; T. Hasunuma; K. Asai; Jun Ishii; A. Kondo

Year: 2020

Venue: Nucleic Acids Research

DOI: https://doi.org/10.1093/nar/gkaa1066

Paper Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris

Peng Cai; Xingpeng Duan; Xiaoyan Wu et al. · 2021 · Nucleic Acids Research · DOI

Full title: Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris

Authors: Peng Cai; Xingpeng Duan; Xiaoyan Wu; Linhui Gao; Min Ye; Yongjin J. Zhou

Year: 2021

Venue: Nucleic Acids Research

DOI: https://doi.org/10.1093/nar/gkab535

Paper Rational engineering of the fungal P450 monooxygenase CYP5136A3 to improve its oxidizing activity toward polycyclic aromatic hydrocarbons

Syed, Khajamohiddin; Porollo, Aleksey; Miller, Dwight W. et al. · 2013 · Protein Engineering Design and Selection · DOI

Full title: Rational engineering of the fungal P450 monooxygenase CYP5136A3 to improve its oxidizing activity toward polycyclic aromatic hydrocarbons

Authors: Syed, Khajamohiddin; Porollo, Aleksey; Miller, Dwight W.; Yadav, Jagjit S.

Year: 2013

Venue: Protein Engineering Design and Selection

DOI: https://doi.org/10.1093/protein/gzt036

Paper Blocking the tunnel: engineering of Candida rugosa lipase mutants with short chain length specificity

Schmitt, Jutta; Brocca, Stefania; Schmid, Rolf D. et al. · 2002 · Protein Engineering Design and Selection · DOI

Full title: Blocking the tunnel: engineering of Candida rugosa lipase mutants with short chain length specificity

Authors: Schmitt, Jutta; Brocca, Stefania; Schmid, Rolf D.; Pleiss, Jürgen

Year: 2002

Venue: Protein Engineering Design and Selection

DOI: https://doi.org/10.1093/protein/15.7.595

Paper Biased mutagenesis in the N-terminal region by degenerate oligonucleotide gene shuffling enhances secretory expression of barley α-amylase 2 in yeast

Fukuda, Kenji; Jensen, Malene H.; Haser, Richard et al. · 2005 · Protein Engineering Design and Selection · DOI

Full title: Biased mutagenesis in the N-terminal region by degenerate oligonucleotide gene shuffling enhances secretory expression of barley α-amylase 2 in yeast

Authors: Fukuda, Kenji; Jensen, Malene H.; Haser, Richard; Aghajari, N.; Svensson, Birte

Year: 2005

Venue: Protein Engineering Design and Selection

DOI: https://doi.org/10.1093/protein/gzi057

Paper Engineering of the upper hinge region of human IgG1 Fc enhances the binding affinity to Fc&ggr;IIIa (CD16a) receptor isoform

Dana Ashoor; Noureddine Ben Khalaf; S. Bourguiba-Hachemi et al. · 2018 · Protein engineering, design & selection : PEDS · DOI

Full title: Engineering of the upper hinge region of human IgG1 Fc enhances the binding affinity to Fc&ggr;IIIa (CD16a) receptor isoform

Authors: Dana Ashoor; Noureddine Ben Khalaf; S. Bourguiba-Hachemi; Maryam Marzouq; M. Fathallah

Year: 2018

Venue: Protein engineering, design & selection : PEDS

DOI: https://doi.org/10.1093/protein/gzy019

Paper Cloning and functional analysis of a fructosyltransferase cDNA for synthesis of highly polymerized levans in timothy (Phleum pratense L.)

Tamura, Ken‐ichi; Kawakami, Akira; Sanada, Yoshinori et al. · 2009 · Journal of Experimental Botany · DOI

Full title: Cloning and functional analysis of a fructosyltransferase cDNA for synthesis of highly polymerized levans in timothy (Phleum pratense L.)

Authors: Tamura, Ken‐ichi; Kawakami, Akira; Sanada, Yoshinori; Tase, Kazuhiro; Komatsu, Toshinori; Yoshida, M.

Year: 2009

Venue: Journal of Experimental Botany

DOI: https://doi.org/10.1093/jxb/ern337

Paper Single-Chain Fv with Fc Fragment of the Human IgG1 Tag: Construction, Pichia pastoris Expression and Antigen Binding Characterization

Andrade, Edmar Vaz de; Albuquerque, Flávia Caixeta; Moraes, Lídia Maria Pepe de et al. · 2000 · The Journal of Biochemistry · DOI

Full title: Single-Chain Fv with Fc Fragment of the Human IgG1 Tag: Construction, Pichia pastoris Expression and Antigen Binding Characterization

Authors: Andrade, Edmar Vaz de; Albuquerque, Flávia Caixeta; Moraes, Lídia Maria Pepe de; Brígido, Marcelo M.; Santos-Silva, Marcos A.

Year: 2000

Venue: The Journal of Biochemistry

DOI: https://doi.org/10.1093/oxfordjournals.jbchem.a022838

Paper Promoter library designed for fine-tuned gene expression in Pichia pastoris

F. Hartner; C. Ruth; David Langenegger et al. · 2008 · Nucleic Acids Research · DOI

Full title: Promoter library designed for fine-tuned gene expression in Pichia pastoris

Authors: F. Hartner; C. Ruth; David Langenegger; Sabrina N. Johnson; P. Hyka; Geoff P. Lin-Cereghino; Joan Lin-Cereghino; K. Kovar; J. Cregg; A. Glieder

Year: 2008

Venue: Nucleic Acids Research

DOI: https://doi.org/10.1093/nar/gkn369

Paper Protein stability: a crystallographer's perspective

Deller, Marc C.; Kong, Leopold; Rupp, Bernhard · 2016 · Acta Crystallographica Section F Structural Biology Communications · DOI

Full title: Protein stability: a crystallographer's perspective

Authors: Deller, Marc C.; Kong, Leopold; Rupp, Bernhard

Year: 2016

Venue: Acta Crystallographica Section F Structural Biology Communications

DOI: https://doi.org/10.1107/s2053230x15024619

Paper Improving industrial yeast strains: exploiting natural and artificial diversity

Steensels, Jan; Snoek, Tim; Meersman, Esther et al. · 2014 · FEMS Microbiology Reviews · DOI

Full title: Improving industrial yeast strains: exploiting natural and artificial diversity

Authors: Steensels, Jan; Snoek, Tim; Meersman, Esther; Nicolino, Martina Picca; Voordeckers, Karin; Verstrepen, Kevin J.

Year: 2014

Venue: FEMS Microbiology Reviews

DOI: https://doi.org/10.1111/1574-6976.12073

Paper Characterization ofLeMir, a Root-Knot Nematode-Induced Gene in Tomato with an Encoded Product Secreted from the Root1

Brenner, Eric D.; Lambert, Kris N.; Kaloshian, Isgouhi et al. · 1998 · PLANT PHYSIOLOGY · DOI

Full title: Characterization ofLeMir, a Root-Knot Nematode-Induced Gene in Tomato with an Encoded Product Secreted from the Root1

Authors: Brenner, Eric D.; Lambert, Kris N.; Kaloshian, Isgouhi; Williamson, Valerie M.

Year: 1998

Venue: PLANT PHYSIOLOGY

DOI: https://doi.org/10.1104/pp.118.1.237

Paper Molecular optimization of rabies virus glycoprotein expression in Pichia pastoris

Safa Ben Azoun; Aicha Eya Belhaj; Rebecca Göngrich et al. · 2016 · Microbial Biotechnology · DOI

Full title: Molecular optimization of rabies virus glycoprotein expression in Pichia pastoris

Authors: Safa Ben Azoun; Aicha Eya Belhaj; Rebecca Göngrich; Brigitte Gasser; H. Kallel

Year: 2016

Venue: Microbial Biotechnology

DOI: https://doi.org/10.1111/1751-7915.12350

Paper Synergic kinetic and physiological control to improve the efficiency of Komagataella phaffii recombinant protein production bioprocesses

Albert Sales-Vallverdú; Arnau Gasset; Guillermo Requena-Moreno et al. · 2024 · Microbial Biotechnology · DOI

Full title: Synergic kinetic and physiological control to improve the efficiency of Komagataella phaffii recombinant protein production bioprocesses

Authors: Albert Sales-Vallverdú; Arnau Gasset; Guillermo Requena-Moreno; Francisco Valero; José Luis Montesinos-Seguí; Xavier Garcia-Ortega

Year: 2024

Venue: Microbial Biotechnology

DOI: https://doi.org/10.1111/1751-7915.14411

Paper Paving the way for the production of secretory proteins by yeast cell factories

M. Huertas; C. Michán · 2019 · Microbial Biotechnology · DOI

Full title: Paving the way for the production of secretory proteins by yeast cell factories

Authors: M. Huertas; C. Michán

Year: 2019

Venue: Microbial Biotechnology

DOI: https://doi.org/10.1111/1751-7915.13342

Paper The secretory pathway: exploring yeast diversity

Delic, Marizela; Valli, Minoska; Graf, Alexandra B. et al. · 2013 · FEMS Microbiology Reviews · DOI

Full title: The secretory pathway: exploring yeast diversity

Authors: Delic, Marizela; Valli, Minoska; Graf, Alexandra B.; Pfeffer, Martin; Mattanovich, Diethard; Gasser, Brigitte

Year: 2013

Venue: FEMS Microbiology Reviews

DOI: https://doi.org/10.1111/1574-6976.12020

Paper Improved pectinase production in Penicillium griseoroseum recombinant strains

Teixeira, J. A.; Gonçalves, Daniel Bonoto; Queiroz, Marisa Vieira de et al. · 2011 · Journal of Applied Microbiology · DOI

Full title: Improved pectinase production in Penicillium griseoroseum recombinant strains

Authors: Teixeira, J. A.; Gonçalves, Daniel Bonoto; Queiroz, Marisa Vieira de; Araújo, E.F.

Year: 2011

Venue: Journal of Applied Microbiology

DOI: https://doi.org/10.1111/j.1365-2672.2011.05099.x

Paper Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris

Y. Ito; M. Ishigami; Noriko Hashiba et al. · 2022 · Microbial Biotechnology · DOI

Full title: Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris

Authors: Y. Ito; M. Ishigami; Noriko Hashiba; Yasuyuki Nakamura; G. Terai; T. Hasunuma; Jun Ishii; Akihiko Kondo

Year: 2022

Venue: Microbial Biotechnology

DOI: https://doi.org/10.1111/1751-7915.14061

Paper NmDef02, a novel antimicrobial gene isolated from Nicotiana megalosiphon confers high‐level pathogen resistance under greenhouse and field conditions

Portieles, Roxana; Ayra‐Pardo, Camilo; González, Ernesto et al. · 2010 · Plant Biotechnology Journal · DOI

Full title: NmDef02, a novel antimicrobial gene isolated from Nicotiana megalosiphon confers high‐level pathogen resistance under greenhouse and field conditions

Authors: Portieles, Roxana; Ayra‐Pardo, Camilo; González, Ernesto; Gallo, Araíz; Rodríguez, Raisa; Chacón, Osmany; López, Yunior; Rodríguez, M.; Castillo, Juan Luis; Pujol, Merardo; Enríquez, Gil; Borroto, Carlos; Trujillo, Luis E; Thomma, Bart P. H. J.; Borrás‐Hidalgo, Orlando

Year: 2010

Venue: Plant Biotechnology Journal

DOI: https://doi.org/10.1111/j.1467-7652.2010.00501.x

Paper Cell-surface phytase on Pichia pastoris cell wall offers great potential as a feed supplement

Harnpicharnchai, Piyanun; Sornlake, Warasirin; Tang, Kittapong et al. · 2009 · FEMS Microbiology Letters · DOI

Full title: Cell-surface phytase on Pichia pastoris cell wall offers great potential as a feed supplement

Authors: Harnpicharnchai, Piyanun; Sornlake, Warasirin; Tang, Kittapong; Eurwilaichitr, Lily; Tanapongpipat, Sutipa

Year: 2009

Venue: FEMS Microbiology Letters

DOI: https://doi.org/10.1111/j.1574-6968.2009.01811.x

Paper Transcriptomics-Based Identification of Novel Factors Enhancing Heterologous Protein Secretion in Yeasts

Brigitte Gasser; M. Sauer; Michael Maurer et al. · 2007 · Applied and Environmental Microbiology · DOI

Full title: Transcriptomics-Based Identification of Novel Factors Enhancing Heterologous Protein Secretion in Yeasts

Authors: Brigitte Gasser; M. Sauer; Michael Maurer; G. Stadlmayr; D. Mattanovich

Year: 2007

Venue: Applied and Environmental Microbiology

DOI: https://doi.org/10.1128/aem.01196-07

Paper Fine‐tuning the P. pastoris iMT1026 genome‐scale metabolic model for improved prediction of growth on methanol or glycerol as sole carbon sources

Tomàs‐Gamisans, Màrius; Ferrer, Pau; Albiol, Joan · 2017 · Microbial Biotechnology · DOI

Full title: Fine‐tuning the P. pastoris iMT1026 genome‐scale metabolic model for improved prediction of growth on methanol or glycerol as sole carbon sources

Authors: Tomàs‐Gamisans, Màrius; Ferrer, Pau; Albiol, Joan

Year: 2017

Venue: Microbial Biotechnology

DOI: https://doi.org/10.1111/1751-7915.12871

Paper Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?

Bill, Roslyn M. · 2014 · Journal of Pharmacy and Pharmacology · DOI

Full title: Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?

Authors: Bill, Roslyn M.

Year: 2014

Venue: Journal of Pharmacy and Pharmacology

DOI: https://doi.org/10.1111/jphp.12353

Paper Functional Expression of Two Unusual Acidic Peroxygenases from Candolleomyces aberdarensis in Yeasts by Adopting Evolved Secretion Mutations

Patricia Gomez de Santos; M. Hoang; Jan Kiebist et al. · 2021 · Applied and Environmental Microbiology · DOI

Full title: Functional Expression of Two Unusual Acidic Peroxygenases from Candolleomyces aberdarensis in Yeasts by Adopting Evolved Secretion Mutations

Authors: Patricia Gomez de Santos; M. Hoang; Jan Kiebist; H. Kellner; R. Ullrich; K. Scheibner; M. Hofrichter; Christiane Liers; M. Alcalde

Year: 2021

Venue: Applied and Environmental Microbiology

DOI: https://doi.org/10.1128/aem.00878-21

Paper Isolation of a laccase-coding gene from the lignin-degrading fungusPhlebia brevisporaBAFC 633 and heterologous expression inPichia pastoris

Fonseca, María Isabel; Molina, Melisa A.; Winnik, D.L. et al. · 2018 · Journal of Applied Microbiology · DOI

Full title: Isolation of a laccase-coding gene from the lignin-degrading fungusPhlebia brevisporaBAFC 633 and heterologous expression inPichia pastoris

Authors: Fonseca, María Isabel; Molina, Melisa A.; Winnik, D.L.; Busi, María V.; Fariña, Julia I.; Villalba, Laura Lidia; Zapata, Pedro Darío

Year: 2018

Venue: Journal of Applied Microbiology

DOI: https://doi.org/10.1111/jam.13720

Paper A Novel Colletotrichum graminicola Raffinose Oxidase in the AA5 Family

Andberg, Martina; Mollerup, Filip; Parikka, Kirsti et al. · 2017 · Applied and Environmental Microbiology · DOI

Full title: A Novel Colletotrichum graminicola Raffinose Oxidase in the AA5 Family

Authors: Andberg, Martina; Mollerup, Filip; Parikka, Kirsti; Koutaniemi, Sanna; Boer, Harry; Juvonen, Minna; Master, Emma R.; Tenkanen, Maija; Kruus, Kristiina

Year: 2017

Venue: Applied and Environmental Microbiology

DOI: https://doi.org/10.1128/aem.01383-17

Paper Impact of the native‐state stability of human lysozyme variants on protein secretion by Pichia pastoris

Kumita, Janet R.; Johnson, Russell J.; Alcocer, Marcos et al. · 2006 · FEBS Journal · DOI

Full title: Impact of the native‐state stability of human lysozyme variants on protein secretion by Pichia pastoris

Authors: Kumita, Janet R.; Johnson, Russell J.; Alcocer, Marcos; Dumoulin, Mireille; Holmqvist, Fredrik; McCammon, Margaret G.; Robinson, Carol V.; Archer, David B.; Dobson, Christopher M.

Year: 2006

Venue: FEBS Journal

DOI: https://doi.org/10.1111/j.1742-4658.2005.05099.x

Paper Humanization of Yeast to Produce Complex Terminally Sialylated Glycoproteins

Hamilton, Stephen R.; Davidson, Robert C.; Sethuraman, Natarajan et al. · 2006 · Science · DOI

Full title: Humanization of Yeast to Produce Complex Terminally Sialylated Glycoproteins

Authors: Hamilton, Stephen R.; Davidson, Robert C.; Sethuraman, Natarajan; Nett, Juergen H.; Jiang, Youwei; Rios, Sandra; Bobrowicz, Piotr; Stadheim, Terrance A.; Li, Huijuan; Choi, Byung-Kwon; Hopkins, Daniel; Wischnewski, Harry; Roser, Jessica; Mitchell, Teresa; Strawbridge, Rendall R.; Hoopes, Jack; Wildt, Stefan; Gerngross, Tillman U.

Year: 2006

Venue: Science

DOI: https://doi.org/10.1126/science.1130256

Paper Characterization of recombinant human lysosomal beta-hexosaminidases produced in the methylotrophic yeast Pichia pastoris

Espejo, Ángela J.; Mosquera, Ángela; Rodríguez‐López, Alexander et al. · 2016 · Universitas Scientiarum · DOI

Full title: Characterization of recombinant human lysosomal beta-hexosaminidases produced in the methylotrophic yeast Pichia pastoris

Authors: Espejo, Ángela J.; Mosquera, Ángela; Rodríguez‐López, Alexander; Díaz, Dennis; Betrán, Laura Milena; Hernández, Francy Liliana; Alméciga-Díaz, Carlos Javier; Barrera, Luis Alejandro

Year: 2016

Venue: Universitas Scientiarum

DOI: https://doi.org/10.11144/javeriana.sc21-3.corh

Paper Overexpression of an anti-CD3 immunotoxin increases expression and secretion of molecular chaperone BiP/Kar2p by Pichia pastoris.

Liu, Yuan Yi; Woo, Jung Hee; Neville, David M · 2005 · Applied and environmental microbiology · DOI

Full title: Overexpression of an anti-CD3 immunotoxin increases expression and secretion of molecular chaperone BiP/Kar2p by Pichia pastoris.

Authors: Liu, Yuan Yi; Woo, Jung Hee; Neville, David M

Year: 2005

Venue: Applied and environmental microbiology

DOI: https://doi.org/10.1128/AEM.71.9.5332-5340.2005

Paper Combinatorial Mutagenesis and Selection to Understand and Improve Yeast Promoters

Berg, Laila; Strand, Trine Aakvik; Valla, Svein et al. · 2013 · BioMed Research International · DOI

Full title: Combinatorial Mutagenesis and Selection to Understand and Improve Yeast Promoters

Authors: Berg, Laila; Strand, Trine Aakvik; Valla, Svein; Brautaset, Trygve

Year: 2013

Venue: BioMed Research International

DOI: https://doi.org/10.1155/2013/926985

Paper Extending the Serum Half-Life of G-CSF via Fusion with the Domain III of Human Serum Albumin

Zhao, Shuqiang; Zhang, Yu; Tian, Hong et al. · 2013 · BioMed Research International · DOI

Full title: Extending the Serum Half-Life of G-CSF via Fusion with the Domain III of Human Serum Albumin

Authors: Zhao, Shuqiang; Zhang, Yu; Tian, Hong; Chen, Xiaofei; Cai, Di; Yao, Wenbing; Gao, Xiangdong

Year: 2013

Venue: BioMed Research International

DOI: https://doi.org/10.1155/2013/107238

Paper Recombinant Strains Producing Thermomyces lanuginosus Thermostable Lipase and their Use in Heterogeneous Biocatalysis, Including Processes of Low-Temperature Synthesis of Esters

Беклемишев, А. Б.; Пыхтина, М. Б.; Перминова, Л. В. et al. · 2022 · Applied Biochemistry and Microbiology · DOI

Full title: Recombinant Strains Producing Thermomyces lanuginosus Thermostable Lipase and their Use in Heterogeneous Biocatalysis, Including Processes of Low-Temperature Synthesis of Esters

Authors: Беклемишев, А. Б.; Пыхтина, М. Б.; Перминова, Л. В.; Коваленко, Г. А.

Year: 2022

Venue: Applied Biochemistry and Microbiology

DOI: https://doi.org/10.1134/s0003683822080026

Paper Redox Engineering by Ectopic Overexpression of NADH Kinase in Recombinant Pichia pastoris (Komagataella phaffii): Impact on Cell Physiology and Recombinant Production of Secreted Proteins

Màrius Tomàs-Gamisans; C. P. Andrade; Francisco Maresca et al. · 2019 · Applied and Environmental Microbiology · DOI

Full title: Redox Engineering by Ectopic Overexpression of NADH Kinase in Recombinant Pichia pastoris (Komagataella phaffii): Impact on Cell Physiology and Recombinant Production of Secreted Proteins

Authors: Màrius Tomàs-Gamisans; C. P. Andrade; Francisco Maresca; Sergi Monforte; P. Ferrer; J. Albiol

Year: 2019

Venue: Applied and Environmental Microbiology

DOI: https://doi.org/10.1128/aem.02038-19

Paper Role of BGS13 in the Secretory Mechanism of Pichia pastoris

C. Naranjo; Anita Jivan; M. Vo et al. · 2019 · Applied and Environmental Microbiology · DOI

Full title: Role of BGS13 in the Secretory Mechanism of Pichia pastoris

Authors: C. Naranjo; Anita Jivan; M. Vo; Katherine H. de Sa Campos; Jared S. Deyarmin; Ryan M. Hekman; Christina Uribe; Aaron Hang; Kai Her; Michelle Fong; Joyce J. Choi; C. Chou; T. Rabara; G. Myers; Pachai Moua; Der Thor; Douglas D. Risser; C. Vierra; A. Franz; Joan Lin-Cereghino; Geoff P. Lin-Cereghino

Year: 2019

Venue: Applied and Environmental Microbiology

DOI: https://doi.org/10.1128/aem.01615-19

Paper Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived fromAspergillus flavusNSH9 inPichia pastoris

Karim, Kazi Muhammad Rezaul; Husaini, Ahmad; Hossain, Md. Anowar et al. · 2016 · BioMed Research International · DOI

Full title: Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived fromAspergillus flavusNSH9 inPichia pastoris

Authors: Karim, Kazi Muhammad Rezaul; Husaini, Ahmad; Hossain, Md. Anowar; Sing, Ngieng Ngui; Sinang, Fazia Mohd; Hussain, Hasnain; Roslan, Hairul Azman

Year: 2016

Venue: BioMed Research International

DOI: https://doi.org/10.1155/2016/5962028

Paper Recombinant Expression of Pleurocidin cDNA Usingthe Pichia pastoris Expression System

Burrowes, Olive-Jean; Diamond, Gill; Lee, Tung-Ching · 2005 · BioMed Research International · DOI

Full title: Recombinant Expression of Pleurocidin cDNA Usingthe Pichia pastoris Expression System

Authors: Burrowes, Olive-Jean; Diamond, Gill; Lee, Tung-Ching

Year: 2005

Venue: BioMed Research International

DOI: https://doi.org/10.1155/jbb.2005.374

Paper Codon Optimization Significantly Improves the Expression Level ofα-Amylase Gene fromBacillus licheniformisinPichia pastoris

Wang, Jian‐Rong; Li, Yang-Yuan; Liu, Dan-Ni et al. · 2015 · BioMed Research International · DOI

Full title: Codon Optimization Significantly Improves the Expression Level ofα-Amylase Gene fromBacillus licheniformisinPichia pastoris

Authors: Wang, Jian‐Rong; Li, Yang-Yuan; Liu, Dan-Ni; Liu, Jingshan; Li, Peng; Chen, Lizhi; Xu, Shude

Year: 2015

Venue: BioMed Research International

DOI: https://doi.org/10.1155/2015/248680

Paper Expression and Characterization ofGeobacillus stearothermophilusSR74 Recombinantα-Amylase inPichia pastoris

Gandhi, S. R.; Salleh, Abu Bakar; Rahman, Raja Noor Zaliha Raja Abd et al. · 2015 · BioMed Research International · DOI

Full title: Expression and Characterization ofGeobacillus stearothermophilusSR74 Recombinantα-Amylase inPichia pastoris

Authors: Gandhi, S. R.; Salleh, Abu Bakar; Rahman, Raja Noor Zaliha Raja Abd; Leow, Thean Chor; Oslan, Siti Nurbaya

Year: 2015

Venue: BioMed Research International

DOI: https://doi.org/10.1155/2015/529059

Paper Comparative Evaluation of Recombinant Protein Production in Different Biofactories: The Green Perspective

Merlin, Matilde; Gecchele, Elisa; Capaldi, Stefano et al. · 2014 · BioMed Research International · DOI

Full title: Comparative Evaluation of Recombinant Protein Production in Different Biofactories: The Green Perspective

Authors: Merlin, Matilde; Gecchele, Elisa; Capaldi, Stefano; Pezzotti, Mario; Avesani, Linda

Year: 2014

Venue: BioMed Research International

DOI: https://doi.org/10.1155/2014/136419

Paper Cloning and Expression of Synthetic Genes Encoding the Broad Antimicrobial Spectrum Bacteriocins SRCAM 602, OR-7, E-760, and L-1077, by Recombinant Pichia pastoris

Arbulu, Sara; Jiménez, Juan J.; Gútiez, Loreto et al. · 2015 · BioMed Research International · DOI

Full title: Cloning and Expression of Synthetic Genes Encoding the Broad Antimicrobial Spectrum Bacteriocins SRCAM 602, OR-7, E-760, and L-1077, by Recombinant Pichia pastoris

Authors: Arbulu, Sara; Jiménez, Juan J.; Gútiez, Loreto; Cintas, Luis M.; Herranz, Carmen; Hernández, Pablo E.

Year: 2015

Venue: BioMed Research International

DOI: https://doi.org/10.1155/2015/767183

Paper Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12

Alias, Norsyuhada; Mazian, Muadz Ahmad; Salleh, Abu Bakar et al. · 2014 · Enzyme Research · DOI

Full title: Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12

Authors: Alias, Norsyuhada; Mazian, Muadz Ahmad; Salleh, Abu Bakar; Basri, Mahiran; Rahman, Raja Noor Zaliha Raja Abd

Year: 2014

Venue: Enzyme Research

DOI: https://doi.org/10.1155/2014/197938

Paper Effects of biodiesel waste glycerol on the growth characteristics of Pichia pastoris genetically modified to produce spidroin.

Cui, Xuemei; Ellison, Michael S. · 2012 · International Journal of ChemTech Research

Full title: Effects of biodiesel waste glycerol on the growth characteristics of Pichia pastoris genetically modified to produce spidroin.

Authors: Cui, Xuemei; Ellison, Michael S.

Year: 2012

Venue: International Journal of ChemTech Research

Paper Lipidomics studies of recombinant Pichia pastoris for improved recombinant protein secretion through cell engineering

N. Vallvé · 2016 · TDX (Tesis Doctorals en Xarxa)

Full title: Lipidomics studies of recombinant Pichia pastoris for improved recombinant protein secretion through cell engineering

Authors: N. Vallvé

Year: 2016

Venue: TDX (Tesis Doctorals en Xarxa)

Paper GAP promoter library for fine-tuning 1 gene expression in Pichia pastoris 2 3 Running title : GAP promoter library for fine-tuning gene expression 4 5

Xiulin Qin; Jiangchao Qian; Gaofeng Yao et al. · 2011

Full title: GAP promoter library for fine-tuning 1 gene expression in Pichia pastoris 2 3 Running title : GAP promoter library for fine-tuning gene expression 4 5

Authors: Xiulin Qin; Jiangchao Qian; Gaofeng Yao; Y. Zhuang; Siliang Zhang; Ju; Chu

Year: 2011

Paper F gene and HN gene co-express of Newcastle disease virus strain F48E9 in Pichia pastoris

Luo-Dan Cheng · 2011

Full title: F gene and HN gene co-express of Newcastle disease virus strain F48E9 in Pichia pastoris

Authors: Luo-Dan Cheng

Year: 2011

Paper L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic

Elena Cámara Rey · 2016 · Dialnet (Universidad de la Rioja)

Authors: Elena Cámara Rey

Year: 2016

Venue: Dialnet (Universidad de la Rioja)

Paper [Enhancement of Coprinus cinereus peroxidase in Pichia pastoris by co-expression chaperone PDI and Ero1].

Chen, Fei; Hu, Meirong; Jiang, Xianzhang et al. · 2015 · Sheng wu gong cheng xue bao = Chinese journal of biotechnology

Full title: [Enhancement of Coprinus cinereus peroxidase in Pichia pastoris by co-expression chaperone PDI and Ero1].

Authors: Chen, Fei; Hu, Meirong; Jiang, Xianzhang; Tao, Yong; Huang, Jianzhong

Year: 2015

Venue: Sheng wu gong cheng xue bao = Chinese journal of biotechnology

Paper Microbial Cell Factories Recombinant Production of Bacterial Toxins and Their Derivatives in the Methylotrophic Yeast Pichia Pastoris

C. Gurkan; D. Ellar; Email et al. · 2005

Full title: Microbial Cell Factories Recombinant Production of Bacterial Toxins and Their Derivatives in the Methylotrophic Yeast Pichia Pastoris

Authors: C. Gurkan; D. Ellar; Email; David; J. Ellar

Year: 2005

Paper Expression of the Rhizopus oryzae lipase in Pichia pastoris under the control of the FLD1 promoter

D. Rodriguez · 2007 · TDX (Tesis Doctorals en Xarxa)

Full title: Expression of the Rhizopus oryzae lipase in Pichia pastoris under the control of the FLD1 promoter

Authors: D. Rodriguez

Year: 2007

Venue: TDX (Tesis Doctorals en Xarxa)

Paper [High-level expression of the potential vaccine antigen TSO18 of Taenia solium in Pichia pastoris].

Yuan, Gailing; Cai, Xue‐Peng; Jing, Zhizhong et al. · 2005 · PubMed

Full title: [High-level expression of the potential vaccine antigen TSO18 of Taenia solium in Pichia pastoris].

Authors: Yuan, Gailing; Cai, Xue‐Peng; Jing, Zhizhong; Zheng, Yadong; Luo, Xuenong; Jia, Wan‐Zhong; Li, Hui; Ding, Juntao

Year: 2005

Venue: PubMed

Paper [Genetic engineering of Pichia pastoris expression system for improved secretion of heterologous proteins--a review].

Guan, Bo; Jin, Jian; Li, Huazhong · 2011 · Wei sheng wu xue bao = Acta microbiologica Sinica

Full title: [Genetic engineering of Pichia pastoris expression system for improved secretion of heterologous proteins--a review].

Authors: Guan, Bo; Jin, Jian; Li, Huazhong

Year: 2011

Venue: Wei sheng wu xue bao = Acta microbiologica Sinica

Paper Multicopy integrative expression and optimization of cecropin AD expression in Pichia pastoris

E. Brown; Niu Jun-Bo; Xue Lin-gui et al. · 2020

Full title: Multicopy integrative expression and optimization of cecropin AD expression in Pichia pastoris

Authors: E. Brown; Niu Jun-Bo; Xue Lin-gui; Wu Juanli; Chen Xi-ming; L. Juan; W. Xia; Liu Yang

Year: 2020

Paper Promoter and process engineering for recombinant protein production in Pichia pastoris towards simple, fast and methanol-free cultivation regimes and high product titers

R. Prielhofer; Michaela Reichinger; K. Claes et al. · 2018

Full title: Promoter and process engineering for recombinant protein production in Pichia pastoris towards simple, fast and methanol-free cultivation regimes and high product titers

Authors: R. Prielhofer; Michaela Reichinger; K. Claes; Christoph Kiziak; N. Wagner; Brigitte Gasser; D. Mattanovich

Year: 2018

Paper [Secretory expression of recombinant porcine zona pellucida glycoprotein-3alpha (rpZP3alpha) in Pichia pastoris].

Gao, Yan-ru; Shan-pei, Pan; Xie, Qi-Xuan et al. · 2006 · PubMed

Full title: [Secretory expression of recombinant porcine zona pellucida glycoprotein-3alpha (rpZP3alpha) in Pichia pastoris].

Authors: Gao, Yan-ru; Shan-pei, Pan; Xie, Qi-Xuan; Xiao, Luanjuan; Qiong, Ke; Li, Wenxing; Wei-wei, Shi

Year: 2006

Venue: PubMed

Paper Recombinant gene expression : reviews and protocols

P. Balbás; A. Lorence · 2012

Full title: Recombinant gene expression : reviews and protocols

Authors: P. Balbás; A. Lorence

Year: 2012

Paper Secreted Expression of S-adenosy-L-methionine Synthetase in Pichia pastoris

He, Guangyuan · 2009 · 农业科学与技术(英文版)

Full title: Secreted Expression of S-adenosy-L-methionine Synthetase in Pichia pastoris

Authors: He, Guangyuan

Year: 2009

Venue: 农业科学与技术(英文版)

Paper Purification,Identification and Optimized Expression of Recombinant Human Neuritin in Pichia Pastoris

Huang, Jinbao · 2008 · Journal of Shihezi University

Full title: Purification,Identification and Optimized Expression of Recombinant Human Neuritin in Pichia Pastoris

Authors: Huang, Jinbao

Year: 2008

Venue: Journal of Shihezi University

Paper Systems metabolic engineering for recombinant protein production in pichia pastoris

S. Mercado · 2019 · Dialnet (Universidad de la Rioja)

Full title: Systems metabolic engineering for recombinant protein production in pichia pastoris

Authors: S. Mercado

Year: 2019

Venue: Dialnet (Universidad de la Rioja)

Paper STRATEGY DESIGN FOR THERAPEUTICAL PROTEIN PRODUCTION by Pichia pastoris

P. Çalık · 2013

Full title: STRATEGY DESIGN FOR THERAPEUTICAL PROTEIN PRODUCTION by Pichia pastoris

Authors: P. Çalık

Year: 2013

Paper High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies

Barnard, Gavin C.; Kull, Angela; Sharkey, Nathan S. et al. · 2010 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies

Authors: Barnard, Gavin C.; Kull, Angela; Sharkey, Nathan S.; Shaikh, Seemab S.; Rittenhour, Alissa M.; Burnina, Irina; Jiang, Youwei; Li, Fang; Lynaugh, Heather; Mitchell, Teresa; Nett, Juergen H.; Nylen, Adam; Potgieter, Thomas I.; Prinz, Bianka; Rios, Sandra; Zha, Dongxing; Sethuraman, Natarajan; Stadheim, Terrance A.; Bobrowicz, Piotr

Year: 2010

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-010-0746-1

Paper Introduction of a disulfide bridge enhances the thermostability of a Streptomyces olivaceoviridis xylanase mutant

Yang, Hong; Yao, Bin; Meng, Kun et al. · 2006 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Introduction of a disulfide bridge enhances the thermostability of a Streptomyces olivaceoviridis xylanase mutant

Authors: Yang, Hong; Yao, Bin; Meng, Kun; Wang, Y. R.; Bai, Yingguo; Wu, Ningfeng

Year: 2006

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-006-0188-y

Paper A simplePichia pastorisfermentation and downstream processing strategy for making recombinant pandemic Swine Origin Influenza A virus Hemagglutinin protein

Athmaram, T. N.; Singh, Ajay; Saraswat, Shweta et al. · 2012 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: A simplePichia pastorisfermentation and downstream processing strategy for making recombinant pandemic Swine Origin Influenza A virus Hemagglutinin protein

Authors: Athmaram, T. N.; Singh, Ajay; Saraswat, Shweta; Srivastava, Saurabh; Misra, Princi; Rao, M. Kameswara; Gopalan, Natarajan; Rao, P. Venkata

Year: 2012

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-012-1220-z

Paper Constitutive expression of human angiostatin in Pichia pastoris by high-density cell culture

Zhang, A. L.; Zhang, T. Y.; Luo, J. X. et al. · 2006 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Constitutive expression of human angiostatin in Pichia pastoris by high-density cell culture

Authors: Zhang, A. L.; Zhang, T. Y.; Luo, J. X.; Chen, Senjie; Guan, Wenjun; Fu, C. Y.; Peng, Shiguo; Li, H. L.

Year: 2006

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-006-0175-3

Paper Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement

Zhang, Wenhui; Potter, Karen J.; Plantz, Bradley A. et al. · 2003 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement

Authors: Zhang, Wenhui; Potter, Karen J.; Plantz, Bradley A.; Schlegel, Vicki; Smith, Leonard A.; Meagher, Michael M.

Year: 2003

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-003-0035-3

Paper Genome-scale metabolic model analysis of Pichia pastoris for enhancing the production of S-adenosyl-l-methionine

Bose, Kabilan Subash Chandra; Shah, Mohd Imran; Krishna, Jayachandran et al. · 2023 · Bioprocess and Biosystems Engineering · DOI

Full title: Genome-scale metabolic model analysis of Pichia pastoris for enhancing the production of S-adenosyl-l-methionine

Authors: Bose, Kabilan Subash Chandra; Shah, Mohd Imran; Krishna, Jayachandran; Meenakshisundaram, Sankaranarayanan

Year: 2023

Venue: Bioprocess and Biosystems Engineering

DOI: https://doi.org/10.1007/s00449-023-02913-1

Paper Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells

A. Berlec; B. Štrukelj · 2013 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells

Authors: A. Berlec; B. Štrukelj

Year: 2013

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-013-1235-0

Paper Scale-up fermentation of recombinant Candida rugosa lipase expressed in Pichia pastoris using the GAP promoter

Zhao, Wei; Wang, Jinwen; Deng, Riqiang et al. · 2007 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Scale-up fermentation of recombinant Candida rugosa lipase expressed in Pichia pastoris using the GAP promoter

Authors: Zhao, Wei; Wang, Jinwen; Deng, Riqiang; Wang, Xunzhang

Year: 2007

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-007-0283-8

Paper Directed modification of the Aspergillus usamii β-mannanase to improve its substrate affinity by in silico design and site-directed mutagenesis

Li, Jianfang; Wei, Xi-Huan; Tang, Cunduo et al. · 2014 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Directed modification of the Aspergillus usamii β-mannanase to improve its substrate affinity by in silico design and site-directed mutagenesis

Authors: Li, Jianfang; Wei, Xi-Huan; Tang, Cunduo; Wang, Junqing; Zhao, Mei; Pang, Qingfeng; Wu, Minchen

Year: 2014

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-014-1406-7

Paper Optimization of cell density and dilution rate in Pichia pastoris continuous fermentations for production of recombinant proteins

Zhang, Wenhui; Liu, Chih-Ping; İnan, Mehmet et al. · 2004 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Optimization of cell density and dilution rate in Pichia pastoris continuous fermentations for production of recombinant proteins

Authors: Zhang, Wenhui; Liu, Chih-Ping; İnan, Mehmet; Meagher, Michael M.

Year: 2004

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-004-0155-4

Paper Functional expression of trypsin from Streptomyces griseus by Pichia pastoris

Ling, Zhenmin; Ma, Tengbo; Li, Jianghua et al. · 2012 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Functional expression of trypsin from Streptomyces griseus by Pichia pastoris

Authors: Ling, Zhenmin; Ma, Tengbo; Li, Jianghua; Du, Guocheng; Kang, Zhen; Chen, Jian

Year: 2012

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-012-1172-3

Paper Efficient secretion of lipase r27RCL in Pichia pastoris by enhancing the disulfide bond formation pathway in the endoplasmic reticulum

Sha, Chong; Yu, Xiao‐Wei; Zhang, Meng et al. · 2013 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Efficient secretion of lipase r27RCL in Pichia pastoris by enhancing the disulfide bond formation pathway in the endoplasmic reticulum

Authors: Sha, Chong; Yu, Xiao‐Wei; Zhang, Meng; Xu, Yan

Year: 2013

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-013-1328-9

Paper Disruption of YPS1 and PEP4 genes reduces proteolytic degradation of secreted HSA/PTH in Pichia pastoris GS115

Wu, Min; Shen, Qi; Yang, Yong et al. · 2013 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Disruption of YPS1 and PEP4 genes reduces proteolytic degradation of secreted HSA/PTH in Pichia pastoris GS115

Authors: Wu, Min; Shen, Qi; Yang, Yong; Zhang, Sheng; Wen, Qu; Chen, Jing; Sun, Hongying; Chen, Shuqing

Year: 2013

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-013-1264-8

Paper Enhanced production of Thermomyces lanuginosus lipase in Pichia pastoris via genetic and fermentation strategies

Fang, Zhonggang; Xu, Li; Pan, Dujie et al. · 2014 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Enhanced production of Thermomyces lanuginosus lipase in Pichia pastoris via genetic and fermentation strategies

Authors: Fang, Zhonggang; Xu, Li; Pan, Dujie; Jiao, Liangcheng; Liu, Ziming; Yan, Yunjun

Year: 2014

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-014-1491-7

Paper Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling

Tredwell, Gregory D.; Aw, Rochelle; Edwards-Jones, Bryn et al. · 2017 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling

Authors: Tredwell, Gregory D.; Aw, Rochelle; Edwards-Jones, Bryn; Leak, David J.; Bundy, Jacob G.

Year: 2017

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-017-1904-5

Paper Enhanced expression of endoinulinase from Aspergillus niger by codon optimization in Pichia pastoris and its application in inulooligosaccharide production

He, Miao; Wu, Dan; Wu, Jing et al. · 2013 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Enhanced expression of endoinulinase from Aspergillus niger by codon optimization in Pichia pastoris and its application in inulooligosaccharide production

Authors: He, Miao; Wu, Dan; Wu, Jing; Chen, Jian

Year: 2013

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-013-1341-z

Paper Engineering the supply chain for protein production/secretion in yeasts and mammalian cells

Klein, Tobias; Niklas, Jens; Heinzle, Elmar · 2015 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Engineering the supply chain for protein production/secretion in yeasts and mammalian cells

Authors: Klein, Tobias; Niklas, Jens; Heinzle, Elmar

Year: 2015

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-014-1569-2

Paper Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase

Dong, He; Luo, Wen; Wang, Zhiyuan et al. · 2015 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase

Authors: Dong, He; Luo, Wen; Wang, Zhiyuan; Lv, Pengmei; Yuan, Zhenhong

Year: 2015

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-015-1633-6

Paper Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the yeast Pichia pastoris.

Bankefa, Olufemi Emmanuel; Wang, Meiyu; Zhu, Taicheng et al. · 2018 · Biotechnology letters · DOI

Full title: Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the yeast Pichia pastoris.

Authors: Bankefa, Olufemi Emmanuel; Wang, Meiyu; Zhu, Taicheng; Li, Yin

Year: 2018

Venue: Biotechnology letters

DOI: https://doi.org/10.1007/s10529-018-2571-y

Paper Engineering the regulatory site of the catalase promoter for improved heterologous protein production in Pichia pastoris

Luyuan Nong; Yimin Zhang; Yehong Duan et al. · 2020 · Biotechnology Letters · DOI

Full title: Engineering the regulatory site of the catalase promoter for improved heterologous protein production in Pichia pastoris

Authors: Luyuan Nong; Yimin Zhang; Yehong Duan; Shulin Hu; Ying-Wu Lin; Shuli Liang

Year: 2020

Venue: Biotechnology Letters

DOI: https://doi.org/10.1007/s10529-020-02979-x

Paper A multistrategy approach for improving the expression of E. coli phytase in Pichia pastoris

Helian, Yuankun; Gai, Yuanming; Fang, Huan et al. · 2020 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: A multistrategy approach for improving the expression of E. coli phytase in Pichia pastoris

Authors: Helian, Yuankun; Gai, Yuanming; Fang, Huan; Sun, Yumei; Zhang, Dawei

Year: 2020

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-020-02311-6

Paper The future of self-selecting and stable fermentations

Rugbjerg, Peter; Olsson, Lisbeth · 2020 · Journal of Industrial Microbiology & Biotechnology · DOI

Full title: The future of self-selecting and stable fermentations

Authors: Rugbjerg, Peter; Olsson, Lisbeth

Year: 2020

Venue: Journal of Industrial Microbiology & Biotechnology

DOI: https://doi.org/10.1007/s10295-020-02325-0

Paper Enhanced protein production by sorbitol co-feeding with methanol in recombinant Pichia pastoris strains

Chen, Li; Mohsin, Ali; Chu, Ju et al. · 2017 · Biotechnology and Bioprocess Engineering · DOI

Full title: Enhanced protein production by sorbitol co-feeding with methanol in recombinant Pichia pastoris strains

Authors: Chen, Li; Mohsin, Ali; Chu, Ju; Zhuang, Yingping; Liu, Yamei; Guo, Meijin

Year: 2017

Venue: Biotechnology and Bioprocess Engineering

DOI: https://doi.org/10.1007/s12257-017-0011-9

Paper A Systematic Review of the Potential of Pichia pastoris (Komagataella phaffii) as an Alternative Host for Biologics Production

Vijay Elakkya Vijayakumar; Krishnan Venkataraman · 2023 · Molecular Biotechnology · DOI

Full title: A Systematic Review of the Potential of Pichia pastoris (Komagataella phaffii) as an Alternative Host for Biologics Production

Authors: Vijay Elakkya Vijayakumar; Krishnan Venkataraman

Year: 2023

Venue: Molecular Biotechnology

DOI: https://doi.org/10.1007/s12033-023-00803-1

Paper Optimization of the secretory expression of recombinant human C-reactive protein in Pichia pastoris

Junming Li; Chengming Sun; Lei Chen et al. · 2017 · 3 Biotech · DOI

Full title: Optimization of the secretory expression of recombinant human C-reactive protein in Pichia pastoris

Authors: Junming Li; Chengming Sun; Lei Chen; Lihui Sun; Lijun Duan; Qing-Wen Zheng; Xue-Jun Hu

Year: 2017

Venue: 3 Biotech

DOI: https://doi.org/10.1007/s13205-017-0917-0

Paper High-level expression of Thermomyces dupontii thermo-alkaline lipase in Pichia pastoris under the control of different promoters

Wang, Jianrong; Zhang, Tianyu; Li, Yangyuan et al. · 2019 · 3 Biotech · DOI

Full title: High-level expression of Thermomyces dupontii thermo-alkaline lipase in Pichia pastoris under the control of different promoters

Authors: Wang, Jianrong; Zhang, Tianyu; Li, Yangyuan; Li, Lilang; Wang, Yong; Yang, Bo; Wang, Yonghua

Year: 2019

Venue: 3 Biotech

DOI: https://doi.org/10.1007/s13205-018-1531-5

Paper Activation of the Unfolded Protein Response via Co-expression of the HAC1i Gene Enhances Expression of Recombinant Elastase in Pichia pastoris

Minghai Han; Weixian Wang; Jianli Zhou et al. · 2020 · Biotechnology and Bioprocess Engineering · DOI

Full title: Activation of the Unfolded Protein Response via Co-expression of the HAC1i Gene Enhances Expression of Recombinant Elastase in Pichia pastoris

Authors: Minghai Han; Weixian Wang; Jianli Zhou; Xun Gong; Cunbin Xu; Yinfeng Li; Qiang Li

Year: 2020

Venue: Biotechnology and Bioprocess Engineering

DOI: https://doi.org/10.1007/s12257-019-0381-2

Paper Tailoring in fungi for next generation cellulase production with special reference to CRISPR/CAS system

Mondal, Subhadeep; Halder, Suman Kumar; Mondal, Keshab Chandra · 2021 · Systems Microbiology and Biomanufacturing · DOI

Full title: Tailoring in fungi for next generation cellulase production with special reference to CRISPR/CAS system

Authors: Mondal, Subhadeep; Halder, Suman Kumar; Mondal, Keshab Chandra

Year: 2021

Venue: Systems Microbiology and Biomanufacturing

DOI: https://doi.org/10.1007/s43393-021-00045-9

Paper Cultivation strategies to enhance productivity of Pichia pastoris: A review

Looser, Verena; Brühlmann, Béla; Bumbak, Fabian et al. · 2015 · Biotechnology Advances · DOI

Full title: Cultivation strategies to enhance productivity of Pichia pastoris: A review

Authors: Looser, Verena; Brühlmann, Béla; Bumbak, Fabian; Stenger, Cynthia L.; Costa, Meritxell; Camattari, Andrea; Fotiadis, Dimitrios; Kovar, Karin

Year: 2015

Venue: Biotechnology Advances

DOI: https://doi.org/10.1016/j.biotechadv.2015.05.008

Paper Hybrid-architectured promoter design to deregulate expression in yeast.

Burcu Gündüz Ergün; P. Çalık · 2021 · Methods in Enzymology · DOI

Full title: Hybrid-architectured promoter design to deregulate expression in yeast.

Authors: Burcu Gündüz Ergün; P. Çalık

Year: 2021

Venue: Methods in Enzymology

DOI: https://doi.org/10.1016/bs.mie.2021.05.014

Paper Challenges and progress towards industrial recombinant protein production in yeasts: A review

Brabander, Pieter De; Uitterhaegen, Evelien; Delmulle, Tom et al. · 2023 · Biotechnology Advances · DOI

Full title: Challenges and progress towards industrial recombinant protein production in yeasts: A review

Authors: Brabander, Pieter De; Uitterhaegen, Evelien; Delmulle, Tom; Winter, Karel De; Soetaert, Wim

Year: 2023

Venue: Biotechnology Advances

DOI: https://doi.org/10.1016/j.biotechadv.2023.108121

Paper Hybrid-architectured promoter design to engineer expression in yeast.

Burcu Gündüz Ergün; P. Çalık · 2021 · Methods in Enzymology · DOI

Full title: Hybrid-architectured promoter design to engineer expression in yeast.

Authors: Burcu Gündüz Ergün; P. Çalık

Year: 2021

Venue: Methods in Enzymology

DOI: https://doi.org/10.1016/bs.mie.2021.05.009

Paper Construction, expression and characterization of a fusion protein HBscFv-IFNγ in Komagatella (Pichia) pastoris X33

Liang, Ming‐Hua; Zhou, Shishui; Jiang, Jian‐Guo · 2017 · Enzyme and Microbial Technology · DOI

Full title: Construction, expression and characterization of a fusion protein HBscFv-IFNγ in Komagatella (Pichia) pastoris X33

Authors: Liang, Ming‐Hua; Zhou, Shishui; Jiang, Jian‐Guo

Year: 2017

Venue: Enzyme and Microbial Technology

DOI: https://doi.org/10.1016/j.enzmictec.2017.04.001

Paper Engineering protein translocation pathway to improve recombinant proteins in Pichia pastoris

Wang, Shengyan; Dai, Huijia; Tang, Qingling et al. · 2024 · Current Research in Biotechnology · DOI

Full title: Engineering protein translocation pathway to improve recombinant proteins in Pichia pastoris

Authors: Wang, Shengyan; Dai, Huijia; Tang, Qingling; Yu, Yujing; Xie, Yaying; Wang, Tao; Huang, Yide

Year: 2024

Venue: Current Research in Biotechnology

DOI: https://doi.org/10.1016/j.crbiot.2024.100182

Paper A review of metabolic and enzymatic engineering strategies for designing and optimizing performance of microbial cell factories

Fisher, Amanda K.; Freedman, Benjamin G.; Bevan, David R. et al. · 2014 · Computational and Structural Biotechnology Journal · DOI

Full title: A review of metabolic and enzymatic engineering strategies for designing and optimizing performance of microbial cell factories

Authors: Fisher, Amanda K.; Freedman, Benjamin G.; Bevan, David R.; Senger, Ryan S.

Year: 2014

Venue: Computational and Structural Biotechnology Journal

DOI: https://doi.org/10.1016/j.csbj.2014.08.010

Paper Dual-acting single-engineered hybrid-architectured promoters enhance and convert expressions into multi-carbon source-regulated systems in Komagataella phaffii.

Beste Avcı; P. Çalık · 2025 · Enzyme and Microbial Technology · DOI

Full title: Dual-acting single-engineered hybrid-architectured promoters enhance and convert expressions into multi-carbon source-regulated systems in Komagataella phaffii.

Authors: Beste Avcı; P. Çalık

Year: 2025

Venue: Enzyme and Microbial Technology

DOI: https://doi.org/10.1016/j.enzmictec.2025.110713

Paper New yeast expression platforms based on methylotrophic and and on dimorphic and – A comparison

Gellissen, Gerd; Kunze, Gotthard; Gaillardin, C. et al. · 2005 · FEMS Yeast Research · DOI

Full title: New yeast expression platforms based on methylotrophic and and on dimorphic and – A comparison

Authors: Gellissen, Gerd; Kunze, Gotthard; Gaillardin, C.; Cregg, James M.; Berardi, Enrico; Veenhuis, Marten; Klei, Ida J. van der

Year: 2005

Venue: FEMS Yeast Research

DOI: https://doi.org/10.1016/j.femsyr.2005.06.004

Paper Overexpression of the Neocallimastix frontalis xylanase gene in the methylotrophic yeasts Pichia pastoris and Pichia methanolica

Tsai, Chang Ting; Huang, Ching‐Tsan · 2008 · Enzyme and Microbial Technology · DOI

Full title: Overexpression of the Neocallimastix frontalis xylanase gene in the methylotrophic yeasts Pichia pastoris and Pichia methanolica

Authors: Tsai, Chang Ting; Huang, Ching‐Tsan

Year: 2008

Venue: Enzyme and Microbial Technology

DOI: https://doi.org/10.1016/j.enzmictec.2008.01.018

Paper Cloning and characterization of the gene as a selectable marker

Thor, Der; Xiong, Sidong; Orazem, Claire C. et al. · 2005 · FEMS Yeast Research · DOI

Full title: Cloning and characterization of the gene as a selectable marker

Authors: Thor, Der; Xiong, Sidong; Orazem, Claire C.; Kwan, Andy C. C.; Cregg, James M.; Lin‐Cereghino, Joan; Lin‐Cereghino, Geoff P.

Year: 2005

Venue: FEMS Yeast Research

DOI: https://doi.org/10.1016/j.femsyr.2005.03.009

Paper Neutral protease expression and optimized conditions for the degradation of blood cells using recombinant Pichia pastoris

Hao, Zhang; Zhang, Boru; Zheng, Yanbin et al. · 2014 · International Biodeterioration & Biodegradation · DOI

Full title: Neutral protease expression and optimized conditions for the degradation of blood cells using recombinant Pichia pastoris

Authors: Hao, Zhang; Zhang, Boru; Zheng, Yanbin; Shan, Anshan; Cheng, Baojing

Year: 2014

Venue: International Biodeterioration & Biodegradation

DOI: https://doi.org/10.1016/j.ibiod.2014.05.024

Paper Overexpression of Candida rugosa lipase Lip1 via combined strategies in Pichia pastoris.

Li, Xu; Liu, Zimin; Wang, Guilong et al. · 2016 · Enzyme and microbial technology · DOI

Full title: Overexpression of Candida rugosa lipase Lip1 via combined strategies in Pichia pastoris.

Authors: Li, Xu; Liu, Zimin; Wang, Guilong; Pan, Dujie; Jiao, Liangcheng; Yan, Yunjun

Year: 2016

Venue: Enzyme and microbial technology

DOI: https://doi.org/10.1016/j.enzmictec.2015.09.003

Paper Pichia pastoris Protein Disulfide Isomerase (PDI1) promoter for heterologous protein production and its sequence characterization.

Prattipati, Mahesh; Ramakrishnan, Kamatchi; Sankaranarayanan, Meenakshisundaram · 2020 · Enzyme and microbial technology · DOI

Full title: Pichia pastoris Protein Disulfide Isomerase (PDI1) promoter for heterologous protein production and its sequence characterization.

Authors: Prattipati, Mahesh; Ramakrishnan, Kamatchi; Sankaranarayanan, Meenakshisundaram

Year: 2020

Venue: Enzyme and microbial technology

DOI: https://doi.org/10.1016/j.enzmictec.2020.109633

Paper Expression, optimization and biological activity analysis of recombinant type XII collagen in Pichia pastoris

Gao, Qiaoyan; Zhang, Zhuo; Fu, Rongzhan et al. · 2025 · International Journal of Biological Macromolecules · DOI

Full title: Expression, optimization and biological activity analysis of recombinant type XII collagen in Pichia pastoris

Authors: Gao, Qiaoyan; Zhang, Zhuo; Fu, Rongzhan; Zhu, Chenhui; Yuwen, Weigang; Ru, Xu; Duan, Zhiguang; Fan, Daidi

Year: 2025

Venue: International Journal of Biological Macromolecules

DOI: https://doi.org/10.1016/j.ijbiomac.2025.143720

Paper Reliable high-throughput screening with by limiting yeast cell death phenomena

Weis, Robert; Luiten, Ruud; Skranc, Wolfgang et al. · 2004 · FEMS Yeast Research · DOI

Full title: Reliable high-throughput screening with by limiting yeast cell death phenomena

Authors: Weis, Robert; Luiten, Ruud; Skranc, Wolfgang; Schwab, Helmut; Wubbolts, M.; Glieder, Anton

Year: 2004

Venue: FEMS Yeast Research

DOI: https://doi.org/10.1016/j.femsyr.2004.06.016

Paper An engineered Pichia pastoris platform for the biosynthesis of silk-based nanomaterials with therapeutic potential.

Tian, Ernuo; Shen, Xiao; Xiao, Meili et al. · 2024 · International journal of biological macromolecules · DOI

Full title: An engineered Pichia pastoris platform for the biosynthesis of silk-based nanomaterials with therapeutic potential.

Authors: Tian, Ernuo; Shen, Xiao; Xiao, Meili; Zhu, Zhihua; Yang, Yi; Yan, Xing; Wang, Pingping; Zou, Gen; Zhou, Zhihua

Year: 2024

Venue: International journal of biological macromolecules

DOI: https://doi.org/10.1016/j.ijbiomac.2024.131954

Paper Defining the mechanism of PDI interaction with disulfide-free amyloidogenic proteins: Implications for exogenous protein expression and neurodegenerative disease.

Yan, Pingyu; Zou, Zhiyuan; Zhang, Shiyao et al. · 2021 · International journal of biological macromolecules · DOI

Full title: Defining the mechanism of PDI interaction with disulfide-free amyloidogenic proteins: Implications for exogenous protein expression and neurodegenerative disease.

Authors: Yan, Pingyu; Zou, Zhiyuan; Zhang, Shiyao; Wang, Rui; Niu, Tingting; Zhang, Xia; Liu, Defu; Zhou, Xuejie; Chang, Alan K; Milton, Nathaniel G N; Jones, Gary W; He, Jianwei

Year: 2021

Venue: International journal of biological macromolecules

DOI: https://doi.org/10.1016/j.ijbiomac.2021.01.172

Paper Engineering of the hypoxia-induced Pichia stipitis ADH2 promoter to construct a promoter library for Pichia pastoris.

Hangcheng Zhou; Zhipeng Wang; Jiangchao Qian · 2023 · Journal of Biotechnology · DOI

Full title: Engineering of the hypoxia-induced Pichia stipitis ADH2 promoter to construct a promoter library for Pichia pastoris.

Authors: Hangcheng Zhou; Zhipeng Wang; Jiangchao Qian

Year: 2023

Venue: Journal of Biotechnology

DOI: https://doi.org/10.1016/j.jbiotec.2023.09.002

Paper Expression and characterization of Gag protein of HIV-1CN in Pichia pastoris

Jiang, Wen Zhi; Jin, Ning; Li, Zi J. et al. · 2004 · Journal of Virological Methods · DOI

Full title: Expression and characterization of Gag protein of HIV-1CN in Pichia pastoris

Authors: Jiang, Wen Zhi; Jin, Ning; Li, Zi J.; Zhang, Li S.; Wang, Hong W.; Zhang, Ying J.; Wen, Han

Year: 2004

Venue: Journal of Virological Methods

DOI: https://doi.org/10.1016/j.jviromet.2004.09.004

Paper Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris.

A. Weninger; Anna-Maria Hatzl; Christian Schmid et al. · 2016 · Journal of Biotechnology · DOI

Full title: Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris.

Authors: A. Weninger; Anna-Maria Hatzl; Christian Schmid; T. Vogl; A. Glieder

Year: 2016

Venue: Journal of Biotechnology

DOI: https://doi.org/10.1016/j.jbiotec.2016.03.027

Paper Intracellular expression and purification of the Canstatin-N protein in Pichia pastoris

Yin, Huixiang; Liu, Zhenwang; Zhang, Ailian et al. · 2012 · Gene · DOI

Full title: Intracellular expression and purification of the Canstatin-N protein in Pichia pastoris

Authors: Yin, Huixiang; Liu, Zhenwang; Zhang, Ailian; Zhang, Tianyuan; Luo, Jinxian; Shen, Jincheng; Chen, Liping; Zhou, Bing; Fu, Xi’an; Fu, C. Y.; Zhang, Zehua

Year: 2012

Venue: Gene

DOI: https://doi.org/10.1016/j.gene.2012.04.073

Paper Effect of molecular chaperones on the expression of Candida antarctica lipase B in Pichia pastoris.

Samuel, Premsingh; Prasanna Vadhana, Ashok Kumar; Kamatchi, Ramakrishnan et al. · 2013 · Microbiological research · DOI

Full title: Effect of molecular chaperones on the expression of Candida antarctica lipase B in Pichia pastoris.

Authors: Samuel, Premsingh; Prasanna Vadhana, Ashok Kumar; Kamatchi, Ramakrishnan; Antony, Aju; Meenakshisundaram, Sankaranarayanan

Year: 2013

Venue: Microbiological research

DOI: https://doi.org/10.1016/j.micres.2013.06.007

Paper Over expression of anti-MUC1 single-domain antibody fragments in the yeast Pichia pastoris

Rahbarizadeh, Fatemeh; Rasaee, Mohammad Javad; Forouzandeh, Mehdi et al. · 2005 · Molecular Immunology · DOI

Full title: Over expression of anti-MUC1 single-domain antibody fragments in the yeast Pichia pastoris

Authors: Rahbarizadeh, Fatemeh; Rasaee, Mohammad Javad; Forouzandeh, Mehdi; Allameh, Abdolamir

Year: 2005

Venue: Molecular Immunology

DOI: https://doi.org/10.1016/j.molimm.2005.03.003

Paper Synthetic activation of yeast stress response improves secretion of recombinant proteins.

Zahrl, Richard J; Prielhofer, Roland; Burgard, Jonas et al. · 2023 · New biotechnology · DOI

Full title: Synthetic activation of yeast stress response improves secretion of recombinant proteins.

Authors: Zahrl, Richard J; Prielhofer, Roland; Burgard, Jonas; Mattanovich, Diethard; Gasser, Brigitte

Year: 2023

Venue: New biotechnology

DOI: https://doi.org/10.1016/j.nbt.2023.01.001

Paper Recombinant expression of a laccase from Coriolopsis gallica in Pichia pastoris using a modified α-factor preproleader.

M. Avelar; C. Olvera; D. Aceves-Zamudio et al. · 2017 · Protein Expression and Purification · DOI

Full title: Recombinant expression of a laccase from Coriolopsis gallica in Pichia pastoris using a modified α-factor preproleader.

Authors: M. Avelar; C. Olvera; D. Aceves-Zamudio; J. Folch; M. Ayala

Year: 2017

Venue: Protein Expression and Purification

DOI: https://doi.org/10.1016/j.pep.2017.06.001

Paper AOX1 promoter-driven expression of yeast-enhanced green fluorescent protein in Pichia pastoris

Dharmarathna, Chethana Sithumini; Kumar, Shashi; Gunawardena, Y. I. Nilmini Silva et al. · 2023 · The Microbe · DOI

Full title: AOX1 promoter-driven expression of yeast-enhanced green fluorescent protein in Pichia pastoris

Authors: Dharmarathna, Chethana Sithumini; Kumar, Shashi; Gunawardena, Y. I. Nilmini Silva; Dassanayake, R. S.; Hettiarachchi, Chamari

Year: 2023

Venue: The Microbe

DOI: https://doi.org/10.1016/j.microb.2023.100012

Paper Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter.

David Resina; Michael Maurer; O. Cós et al. · 2009 · New Biotechnology · DOI

Full title: Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter.

Authors: David Resina; Michael Maurer; O. Cós; C. Arnau; Marc Carnicer; Hans Marx; Brigitte Gasser; F. Valero; D. Mattanovich; P. Ferrer

Year: 2009

Venue: New Biotechnology

DOI: https://doi.org/10.1016/j.nbt.2009.01.008

Paper Bioreactor-scale cell performance and protein production can be substantially increased by using a secretion signal that drives co-translational translocation in Pichia pastoris.

Barrero, Juan J; Pagazartaundua, Alejandro; Glick, Benjamin S et al. · 2021 · New biotechnology · DOI

Full title: Bioreactor-scale cell performance and protein production can be substantially increased by using a secretion signal that drives co-translational translocation in Pichia pastoris.

Authors: Barrero, Juan J; Pagazartaundua, Alejandro; Glick, Benjamin S; Valero, Francisco; Ferrer, Pau

Year: 2021

Venue: New biotechnology

DOI: https://doi.org/10.1016/j.nbt.2020.09.001

Paper Towards improved membrane protein production in Pichia pastoris: general and specific transcriptional response to membrane protein overexpression.

Vogl, Thomas; Thallinger, Gerhard G; Zellnig, Guenther et al. · 2014 · New biotechnology · DOI

Full title: Towards improved membrane protein production in Pichia pastoris: general and specific transcriptional response to membrane protein overexpression.

Authors: Vogl, Thomas; Thallinger, Gerhard G; Zellnig, Guenther; Drew, David; Cregg, James M; Glieder, Anton; Freigassner, Maria

Year: 2014

Venue: New biotechnology

DOI: https://doi.org/10.1016/j.nbt.2014.02.009

Paper Expression of recombinant tachyplesin I in Pichia pastoris

Li, Hanmei; Ali, Zeeshan; Liu, Xiaolong et al. · 2019 · Protein Expression and Purification · DOI

Full title: Expression of recombinant tachyplesin I in Pichia pastoris

Authors: Li, Hanmei; Ali, Zeeshan; Liu, Xiaolong; Jiang, Li; Tang, Yongjun; Dai, Jianguo

Year: 2019

Venue: Protein Expression and Purification

DOI: https://doi.org/10.1016/j.pep.2019.01.012

Paper Hac1p-based inverse secretory pathway engineering (Hi-SPE) of Pichia pastoris for improved glucose oxidase production.

Zhao, Xinyu; Fang, Jiayu; Yu, Sijie et al. · 2025 · New biotechnology · DOI

Full title: Hac1p-based inverse secretory pathway engineering (Hi-SPE) of Pichia pastoris for improved glucose oxidase production.

Authors: Zhao, Xinyu; Fang, Jiayu; Yu, Sijie; Liu, Guoxia; Zhang, Yanping; Li, Yin; Zhu, Taicheng

Year: 2025

Venue: New biotechnology

DOI: https://doi.org/10.1016/j.nbt.2025.07.002

Paper A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris

Xiang Wang; Yi Li; Zhehao Jin et al. · 2023 · Synthetic and Systems Biotechnology · DOI

Full title: A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris

Authors: Xiang Wang; Yi Li; Zhehao Jin; Xiangjian Liu; Xiang Gao; Shuyuan Guo; Tao Yu

Year: 2023

Venue: Synthetic and Systems Biotechnology

DOI: https://doi.org/10.1016/j.synbio.2023.06.003

Paper The combined effect of the gene copy number and chaperone overexpression on the recombinant bovine chymosin production in Pichia pastoris, with mutant ADH2 promoter.

Ersöz, Fatma; İnan, Mehmet · 2025 · Protein expression and purification · DOI

Full title: The combined effect of the gene copy number and chaperone overexpression on the recombinant bovine chymosin production in Pichia pastoris, with mutant ADH2 promoter.

Authors: Ersöz, Fatma; İnan, Mehmet

Year: 2025

Venue: Protein expression and purification

DOI: https://doi.org/10.1016/j.pep.2024.106636

Paper Genome and metabolic engineering in non-conventional yeasts: Current advances and applications.

Löbs, Ann-Kathrin; Schwartz, Cory; Wheeldon, Ian · 2017 · Synthetic and systems biotechnology · DOI

Full title: Genome and metabolic engineering in non-conventional yeasts: Current advances and applications.

Authors: Löbs, Ann-Kathrin; Schwartz, Cory; Wheeldon, Ian

Year: 2017

Venue: Synthetic and systems biotechnology

DOI: https://doi.org/10.1016/j.synbio.2017.08.002

Paper Molecular chaperones: A revolutionary approach for increased solubility of recombinant mAbs from bacterial and yeast systems

Kulkarni, Niharika Ashish; Das, Prabir Kumar; Arjun, P et al. · 2025 · Protein Expression and Purification · DOI

Full title: Molecular chaperones: A revolutionary approach for increased solubility of recombinant mAbs from bacterial and yeast systems

Authors: Kulkarni, Niharika Ashish; Das, Prabir Kumar; Arjun, P; Veeranki, Venkata Dasu

Year: 2025

Venue: Protein Expression and Purification

DOI: https://doi.org/10.1016/j.pep.2025.106764

Paper Rational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagataella phaffi) using the methanol free GAP promoter. Where do we stand?

Xavier Garcia-Ortega; Elena Cámara; P. Ferrer et al. · 2019 · New Biotechnology · DOI

Full title: Rational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagataella phaffi) using the methanol free GAP promoter. Where do we stand?

Authors: Xavier Garcia-Ortega; Elena Cámara; P. Ferrer; J. Albiol; José Luis Montesinos-Seguí; F. Valero

Year: 2019

Venue: New Biotechnology

DOI: https://doi.org/10.1016/j.nbt.2019.06.002

Paper Engineering artificial cross-species promoters with different transcriptional strengths

Wenjie Zuo; Guobin Yin; Luyao Zhang et al. · 2024 · Synthetic and Systems Biotechnology · DOI

Full title: Engineering artificial cross-species promoters with different transcriptional strengths

Authors: Wenjie Zuo; Guobin Yin; Luyao Zhang; Weijiao Zhang; Ruirui Xu; Yang Wang; Jianghua Li; Zhen Kang

Year: 2024

Venue: Synthetic and Systems Biotechnology

DOI: https://doi.org/10.1016/j.synbio.2024.08.003

Paper High-Level Expression of Fusion Protein Containing 10 Tandem Repeated GLP-1 Analogs in YeastPichia pastorisand Its Biological Activity in a Diabetic Rat Model

Hou, Jianhua; Yan, Ruixiang; Yang, Liquan et al. · 2007 · Bioscience Biotechnology and Biochemistry · DOI

Full title: High-Level Expression of Fusion Protein Containing 10 Tandem Repeated GLP-1 Analogs in YeastPichia pastorisand Its Biological Activity in a Diabetic Rat Model

Authors: Hou, Jianhua; Yan, Ruixiang; Yang, Liquan; Wu, Zhiqiang; Wang, Cuiyan; Ding, Dongfeng; Li, Na; Ma, Chunxiao; Li, Minggang

Year: 2007

Venue: Bioscience Biotechnology and Biochemistry

DOI: https://doi.org/10.1271/bbb.60694

Paper Engineering heterologous core promoters to optimize the SES expression system for Pichia pastoris

Bo Sun; Yu Tan; N. Mund et al. · 2025 · Synthetic and Systems Biotechnology · DOI

Full title: Engineering heterologous core promoters to optimize the SES expression system for Pichia pastoris

Authors: Bo Sun; Yu Tan; N. Mund; Ketao Wang; Lirong Yang; Jianping Wu; Jixing Luo; Yuanqiang Li; Ling Jiang; Hao Fang; Haoran Yu

Year: 2025

Venue: Synthetic and Systems Biotechnology

DOI: https://doi.org/10.1016/j.synbio.2025.10.005

Paper Whole-cell (+)-ambrein production in the yeast Pichia pastoris

Moser, Sandra; Strohmeier, Gernot A.; Leitner, Erich et al. · 2018 · Metabolic Engineering Communications · DOI

Full title: Whole-cell (+)-ambrein production in the yeast Pichia pastoris

Authors: Moser, Sandra; Strohmeier, Gernot A.; Leitner, Erich; Plocek, Thomas J.; Vanhessche, Koenraad; Pichler, Harald

Year: 2018

Venue: Metabolic Engineering Communications

DOI: https://doi.org/10.1016/j.mec.2018.e00077

Paper Optimized expression of the Starmerella bombicola lactone esterase in Pichia pastoris through temperature adaptation, codon-optimization and co-expression with HAC1

Waele, Stijn De; Vandenberghe, Isabel; Laukens, Bram et al. · 2017 · Protein Expression and Purification · DOI

Full title: Optimized expression of the Starmerella bombicola lactone esterase in Pichia pastoris through temperature adaptation, codon-optimization and co-expression with HAC1

Authors: Waele, Stijn De; Vandenberghe, Isabel; Laukens, Bram; Planckaert, Sören; Verweire, Stijn; Bogaert, Inge Van; Soetaert, Wim; Devreese, Bart; Ciesielska, Katarzyna

Year: 2017

Venue: Protein Expression and Purification

DOI: https://doi.org/10.1016/j.pep.2017.10.016

Paper Promoter engineering for enhanced 3-hydroxypropionic acid production in Pichia pastoris

Shushu Chen; Mengyao Zhang; Xiaoyan Wu et al. · 2025 · Synthetic and Systems Biotechnology · DOI

Full title: Promoter engineering for enhanced 3-hydroxypropionic acid production in Pichia pastoris

Authors: Shushu Chen; Mengyao Zhang; Xiaoyan Wu; Fan Bai; Linhui Gao; Yiwei Shen; Shaohua Dou; Peng Cai; Yongjin J. Zhou

Year: 2025

Venue: Synthetic and Systems Biotechnology

DOI: https://doi.org/10.1016/j.synbio.2025.04.013

Paper Recent advances in microbial transglutaminase biosynthesis and its application in the food industry

M. Akbari; S. Razavi; M. Kieliszek · 2021 · Trends in Food Science & Technology · DOI

Full title: Recent advances in microbial transglutaminase biosynthesis and its application in the food industry

Authors: M. Akbari; S. Razavi; M. Kieliszek

Year: 2021

Venue: Trends in Food Science & Technology

DOI: https://doi.org/10.1016/j.tifs.2021.02.036

Paper Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

Gao, Jucan; Jiang, Lihong; Lian, Jiazhang · 2021 · Synthetic and Systems Biotechnology · DOI

Full title: Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

Authors: Gao, Jucan; Jiang, Lihong; Lian, Jiazhang

Year: 2021

Venue: Synthetic and Systems Biotechnology

DOI: https://doi.org/10.1016/j.synbio.2021.04.005

Paper Metabolic engineering of Pichia pastoris

Peña, David A.; Gasser, Brigitte; Zanghellini, Jürgen et al. · 2018 · Metabolic Engineering · DOI

Full title: Metabolic engineering of Pichia pastoris

Authors: Peña, David A.; Gasser, Brigitte; Zanghellini, Jürgen; Steiger, Matthias G.; Mattanovich, Diethard

Year: 2018

Venue: Metabolic Engineering

DOI: https://doi.org/10.1016/j.ymben.2018.04.017

Paper Pushing and pulling proteins into the yeast secretory pathway enhances recombinant protein secretion.

Zahrl, Richard J; Prielhofer, Roland; Ata, Özge et al. · 2022 · Metabolic engineering · DOI

Full title: Pushing and pulling proteins into the yeast secretory pathway enhances recombinant protein secretion.

Authors: Zahrl, Richard J; Prielhofer, Roland; Ata, Özge; Baumann, Kristin; Mattanovich, Diethard; Gasser, Brigitte

Year: 2022

Venue: Metabolic engineering

DOI: https://doi.org/10.1016/j.ymben.2022.08.010

Paper Structural and Biochemical Studies Enlighten the Unspecific Peroxygenase from Hypoxylon sp. EC38 as an Efficient Oxidative Biocatalyst

Rotilio, Laura; Swoboda, Alexander; Ebner, Katharina et al. · 2021 · ACS Catalysis · DOI

Full title: Structural and Biochemical Studies Enlighten the Unspecific Peroxygenase from Hypoxylon sp. EC38 as an Efficient Oxidative Biocatalyst

Authors: Rotilio, Laura; Swoboda, Alexander; Ebner, Katharina; Rinnofner, Claudia; Glieder, Anton; Kroutil, Wolfgang; Mattevi, Andrea

Year: 2021

Venue: ACS Catalysis

DOI: https://doi.org/10.1021/acscatal.1c03065

Paper Acid Resistance Engineering of Endoglucanase for the Degradation of Wine Lees.

Ruiyang Hou; Qianli Zhang; Wenmiao Wu et al. · 2024 · Journal of Agricultural and Food Chemistry · DOI

Full title: Acid Resistance Engineering of Endoglucanase for the Degradation of Wine Lees.

Authors: Ruiyang Hou; Qianli Zhang; Wenmiao Wu; Yaping Ma; Rongya Zhang; Minchang Liu; Jian Chen; Wu Wen; Juan Zhang; Zheng Peng

Year: 2024

Venue: Journal of Agricultural and Food Chemistry

DOI: https://doi.org/10.1021/acs.jafc.4c08399

Paper Use of HDEL‐tagged Trichoderma reesei mannosyl oligosaccharide 1,2‐α‐ D ‐mannosidase for N ‐glycan engineering in Pichia pastoris

Callewaert, Nico; Laroy, Wouter; Cadirgi, Hasan et al. · 2001 · FEBS Letters · DOI

Full title: Use of HDEL‐tagged Trichoderma reesei mannosyl oligosaccharide 1,2‐α‐ D ‐mannosidase for N ‐glycan engineering in Pichia pastoris

Authors: Callewaert, Nico; Laroy, Wouter; Cadirgi, Hasan; Geysens, Steven; Saelens, Xavier; Jou, Willy Min; Contreras, Roland

Year: 2001

Venue: FEBS Letters

DOI: https://doi.org/10.1016/s0014-5793(01)02676-x

Paper Establishing Komagataella phaffii as a Cell Factory for Efficient Production of Sesquiterpenoid α-Santalene.

Yimeng Zuo; Feng Xiao; Jucan Gao et al. · 2022 · Journal of Agricultural and Food Chemistry · DOI

Full title: Establishing Komagataella phaffii as a Cell Factory for Efficient Production of Sesquiterpenoid α-Santalene.

Authors: Yimeng Zuo; Feng Xiao; Jucan Gao; Cuifang Ye; Lihong Jiang; Chang Dong; J. Lian

Year: 2022

Venue: Journal of Agricultural and Food Chemistry

DOI: https://doi.org/10.1021/acs.jafc.2c02353

Paper Recombinant Batroxobin Expressed Highly in Pichia pastoris

Li, Zhi; Yu, Xijie; Huang, Jinling et al. · 2007 · Chinese journal of biotechnology/Shengwu gongcheng xuebao · DOI

Full title: Recombinant Batroxobin Expressed Highly in Pichia pastoris

Authors: Li, Zhi; Yu, Xijie; Huang, Jinling; Fang, Hongda; CHEN, H

Year: 2007

Venue: Chinese journal of biotechnology/Shengwu gongcheng xuebao

DOI: https://doi.org/10.1016/s1872-2075(07)60035-1

Paper Heterologous protein expression in the methylotrophic yeast Pichia pastoris

Cereghino, Joan Lin · 2000 · FEMS Microbiology Reviews · DOI

Full title: Heterologous protein expression in the methylotrophic yeast Pichia pastoris

Authors: Cereghino, Joan Lin

Year: 2000

Venue: FEMS Microbiology Reviews

DOI: https://doi.org/10.1016/s0168-6445(99)00029-7

Paper Microbial Astaxanthin Synthesis by Komagataella phaffii through Metabolic and Fermentation Engineering.

Jingnan Wang; Weixu Ma; Wenqi Ma et al. · 2025 · Journal of Agricultural and Food Chemistry · DOI

Full title: Microbial Astaxanthin Synthesis by Komagataella phaffii through Metabolic and Fermentation Engineering.

Authors: Jingnan Wang; Weixu Ma; Wenqi Ma; Zhi Yao; Yujia Jiang; Wankui Jiang; F. Xin; Wenming Zhang; Min Jiang

Year: 2025

Venue: Journal of Agricultural and Food Chemistry

DOI: https://doi.org/10.1021/acs.jafc.4c10113

Paper Fine-Tuned Gene Expression Elements from Hybrid Promoter Libraries in Pichia pastoris.

Jie Lai; Lingang Song; Yuyu Zhou et al. · 2023 · ACS Synthetic Biology · DOI

Full title: Fine-Tuned Gene Expression Elements from Hybrid Promoter Libraries in Pichia pastoris.

Authors: Jie Lai; Lingang Song; Yuyu Zhou; H. Zong; B. Zhuge; Xinyao Lu

Year: 2023

Venue: ACS Synthetic Biology

DOI: https://doi.org/10.1021/acssynbio.3c00534

Paper Codon-tRNA Coadaptation Bias for Identifying Strong Native Promoters in Komagataella phaffii.

L. L. B. Kastberg; M. S. Petrov; Tomas Strucko et al. · 2024 · ACS Synthetic Biology · DOI

Full title: Codon-tRNA Coadaptation Bias for Identifying Strong Native Promoters in Komagataella phaffii.

Authors: L. L. B. Kastberg; M. S. Petrov; Tomas Strucko; M. Jensen; Christopher T Workman

Year: 2024

Venue: ACS Synthetic Biology

DOI: https://doi.org/10.1021/acssynbio.3c00567

Paper Enhancing the Thermostability and Catalytic Activity of the Lipase from Rhizopus oryzae via Introducing N-Glycosylation.

Yong Wang; Ziyuan Wang; Huifen Yu et al. · 2024 · Journal of Agricultural and Food Chemistry · DOI

Full title: Enhancing the Thermostability and Catalytic Activity of the Lipase from Rhizopus oryzae via Introducing N-Glycosylation.

Authors: Yong Wang; Ziyuan Wang; Huifen Yu; Haidong Teng; Jianping Wu; Jiaqi Xu; Lirong Yang

Year: 2024

Venue: Journal of Agricultural and Food Chemistry

DOI: https://doi.org/10.1021/acs.jafc.4c02623

Paper Fine-Tuning of Transcription in Pichia pastoris Using dCas9 and RNA Scaffolds

Michael Baumschabl; R. Prielhofer; D. Mattanovich et al. · 2020 · ACS Synthetic Biology · DOI

Full title: Fine-Tuning of Transcription in Pichia pastoris Using dCas9 and RNA Scaffolds

Authors: Michael Baumschabl; R. Prielhofer; D. Mattanovich; Matthias G. Steiger

Year: 2020

Venue: ACS Synthetic Biology

DOI: https://doi.org/10.1021/acssynbio.0c00214

Paper Enhanced secretion of heterologous proteins in Pichia pastoris following overexpression of Saccharomyces cerevisiae chaperone proteins.

Zhang, Wei; Zhao, Hong-Liang; Xue, Chong et al. · 2006 · Biotechnology progress · DOI

Full title: Enhanced secretion of heterologous proteins in Pichia pastoris following overexpression of Saccharomyces cerevisiae chaperone proteins.

Authors: Zhang, Wei; Zhao, Hong-Liang; Xue, Chong; Xiong, Xiang-hua; Yao, Xue-qin; Li, Xian-yu; Chen, Hui-peng; Liu, Zhi-min

Year: 2006

Venue: Biotechnology progress

DOI: https://doi.org/10.1021/bp060019r

Paper Omics-Based Expression Cassette for Heterologous Protein Production in Y. lipolytica.

Ridwan Elemosho; Chenxi Zhang; Alexandros Karyolaimos et al. · 2026 · ACS Synthetic Biology · DOI

Full title: Omics-Based Expression Cassette for Heterologous Protein Production in Y. lipolytica.

Authors: Ridwan Elemosho; Chenxi Zhang; Alexandros Karyolaimos; Agata Brambor; Raul Pasadas Castilla; Jonathan Asmund Arnesen; L. J. Jahn; M. Sommer

Year: 2026

Venue: ACS Synthetic Biology

DOI: https://doi.org/10.1021/acssynbio.5c00612

Paper One-Step In Vivo Assembly of Multiple DNA Fragments and Genomic Integration in Komagataella phaffii

Nishi, Teruyuki; Ito, Yoichiro; Nakamura, Yasuyuki et al. · 2022 · ACS Synthetic Biology · DOI

Full title: One-Step In Vivo Assembly of Multiple DNA Fragments and Genomic Integration in Komagataella phaffii

Authors: Nishi, Teruyuki; Ito, Yoichiro; Nakamura, Yasuyuki; Yamaji, Taiki; Hashiba, Noriko; Tamai, Masaya; Yasohara, Yoshihiko; Ishii, Jun; Kondo, Akihiko

Year: 2022

Venue: ACS Synthetic Biology

DOI: https://doi.org/10.1021/acssynbio.1c00302

Paper Synthetic Core Promoters for Pichia pastoris

T. Vogl; C. Ruth; Julia Pitzer et al. · 2013 · ACS Synthetic Biology · DOI

Full title: Synthetic Core Promoters for Pichia pastoris

Authors: T. Vogl; C. Ruth; Julia Pitzer; Thomas Kickenweiz; A. Glieder

Year: 2013

Venue: ACS Synthetic Biology

DOI: https://doi.org/10.1021/sb400091p

Paper Synthetic Core Promoters as Universal Parts for Fine-Tuning Expression in Different Yeast Species

Rui M. C. Portela; T. Vogl; Claudia Kniely et al. · 2016 · ACS Synthetic Biology · DOI

Full title: Synthetic Core Promoters as Universal Parts for Fine-Tuning Expression in Different Yeast Species

Authors: Rui M. C. Portela; T. Vogl; Claudia Kniely; J. E. Fischer; Rui Oliveira; A. Glieder

Year: 2016

Venue: ACS Synthetic Biology

DOI: https://doi.org/10.1021/acssynbio.6b00178

Paper Microfluidic Fluorescence-Activated Cell Sorting of Convolutional Neural Network-Designed Synthetic Yeast Promoter.

Zhen Cheng; Wenfa Wu; Xiao Zhou et al. · 2025 · ACS Synthetic Biology · DOI

Full title: Microfluidic Fluorescence-Activated Cell Sorting of Convolutional Neural Network-Designed Synthetic Yeast Promoter.

Authors: Zhen Cheng; Wenfa Wu; Xiao Zhou; Tingdong Xu; Guanbin Zhang; Hongwei Chen

Year: 2025

Venue: ACS Synthetic Biology

DOI: https://doi.org/10.1021/acssynbio.5c00025

Paper Recombinant production of eukaryotic cytochrome P450s in microbial cell factories

Hausjell, Johanna; Halbwirth, Heidi; Spadiut, Oliver · 2018 · Bioscience Reports · DOI

Full title: Recombinant production of eukaryotic cytochrome P450s in microbial cell factories

Authors: Hausjell, Johanna; Halbwirth, Heidi; Spadiut, Oliver

Year: 2018

Venue: Bioscience Reports

DOI: https://doi.org/10.1042/bsr20171290

Paper Glycosylation by Pichia pastoris decreases the affinity of a family 2a carbohydrate-binding module from Cellulomonas fimi: a functional and mutational analysis

Boraston, A.B.; Warren, R. Antony J.; Kilburn, Douglas G. · 2001 · Biochemical Journal · DOI

Full title: Glycosylation by Pichia pastoris decreases the affinity of a family 2a carbohydrate-binding module from Cellulomonas fimi: a functional and mutational analysis

Authors: Boraston, A.B.; Warren, R. Antony J.; Kilburn, Douglas G.

Year: 2001

Venue: Biochemical Journal

DOI: https://doi.org/10.1042/0264-6021:3580423

Paper The C-terminal segment of the 1,3-beta-glucanase Ole e 9 from olive (Olea europaea) pollen is an independent domain with allergenic activity: expression in Pichia pastoris and characterization

Palomares, Óscar; Villalba, Mayte; Rodrı́guez, Rosalı́a · 2003 · Biochemical Journal · DOI

Full title: The C-terminal segment of the 1,3-beta-glucanase Ole e 9 from olive (Olea europaea) pollen is an independent domain with allergenic activity: expression in Pichia pastoris and characterization

Authors: Palomares, Óscar; Villalba, Mayte; Rodrı́guez, Rosalı́a

Year: 2003

Venue: Biochemical Journal

DOI: https://doi.org/10.1042/bj20020423

Paper Multiple mutagenesis of non-universal serine codons of the Candida rugosa LIP2 gene and biochemical characterization of purified recombinant LIP2 lipase overexpressed in Pichia pastoris

LEE, Guan-Chiun; Lee, Li-Chiun; Sava, Vasyl et al. · 2002 · Biochemical Journal · DOI

Full title: Multiple mutagenesis of non-universal serine codons of the Candida rugosa LIP2 gene and biochemical characterization of purified recombinant LIP2 lipase overexpressed in Pichia pastoris

Authors: LEE, Guan-Chiun; Lee, Li-Chiun; Sava, Vasyl; Shaw, J. F.

Year: 2002

Venue: Biochemical Journal

DOI: https://doi.org/10.1042/bj20020404

Paper Substrate (aglycone) specificity of human cytosolic beta-glucosidase

Berrin, Jean‐Guy; Czjzek, Mirjam; Kroon, Paul A. et al. · 2003 · Biochemical Journal · DOI

Full title: Substrate (aglycone) specificity of human cytosolic beta-glucosidase

Authors: Berrin, Jean‐Guy; Czjzek, Mirjam; Kroon, Paul A.; McLauchlan, W.Russell; Puigserver, Antoine; Williamson, Gary; Juge, Nathalie

Year: 2003

Venue: Biochemical Journal

DOI: https://doi.org/10.1042/bj20021876

Paper Propeptide dependent activation of the Antarctic krill euphauserase precursor produced in yeast

Kristjánsdóttir, Sigríður; Guðmundsdóttir, Ágústa · 2000 · European Journal of Biochemistry · DOI

Full title: Propeptide dependent activation of the Antarctic krill euphauserase precursor produced in yeast

Authors: Kristjánsdóttir, Sigríður; Guðmundsdóttir, Ágústa

Year: 2000

Venue: European Journal of Biochemistry

DOI: https://doi.org/10.1046/j.1432-1327.2000.01273.x

Paper Characterization of potato proteinase inhibitor II reactive site mutants

Beekwilder, Jules; Schipper, Bert; Bakker, Petra L. et al. · 2000 · European Journal of Biochemistry · DOI

Full title: Characterization of potato proteinase inhibitor II reactive site mutants

Authors: Beekwilder, Jules; Schipper, Bert; Bakker, Petra L.; Bosch, Dirk; Jongsma, Maarten A.

Year: 2000

Venue: European Journal of Biochemistry

DOI: https://doi.org/10.1046/j.1432-1327.2000.01201.x

Paper Ktr1p is an α-1,2-mannosyltransferase of Saccharomyces cerevisiae. Comparison of the enzymic properties of soluble recombinant Ktr1p and Kre2p/Mnt1p produced in Pichia pastoris

Romero, Pedro; Lussier, Marc; Sdicu, Anne‐Marie et al. · 1997 · Biochemical Journal · DOI

Full title: Ktr1p is an α-1,2-mannosyltransferase of Saccharomyces cerevisiae. Comparison of the enzymic properties of soluble recombinant Ktr1p and Kre2p/Mnt1p produced in Pichia pastoris

Authors: Romero, Pedro; Lussier, Marc; Sdicu, Anne‐Marie; Bussey, Howard; Herscovics, Annetté

Year: 1997

Venue: Biochemical Journal

DOI: https://doi.org/10.1042/bj3210289

Paper Procarboxypeptidase A from the insect pest Helicoverpa armigera and its derived enzyme

Bayés, Àlex; Sonnenschein, Anka; Daura, Xavier et al. · 2003 · European Journal of Biochemistry · DOI

Full title: Procarboxypeptidase A from the insect pest Helicoverpa armigera and its derived enzyme

Authors: Bayés, Àlex; Sonnenschein, Anka; Daura, Xavier; Vendrell, Josep; Avilés, Francesc

Year: 2003

Venue: European Journal of Biochemistry

DOI: https://doi.org/10.1046/j.1432-1033.2003.03681.x

Paper Role of the Occluding Loop in Cathepsin B Activity

Illy, Chantal; Quraishi, Omar; Wang, Jing et al. · 1997 · Journal of Biological Chemistry · DOI

Full title: Role of the Occluding Loop in Cathepsin B Activity

Authors: Illy, Chantal; Quraishi, Omar; Wang, Jing; Purisima, Enrico O.; Vernet, Thierry; Mort, John S.

Year: 1997

Venue: Journal of Biological Chemistry

DOI: https://doi.org/10.1074/jbc.272.2.1197

Paper Structural analysis of the CD5 antigen

McAlister, M.; Brown, Marion H.; Willis, Antony C. et al. · 1998 · European Journal of Biochemistry · DOI

Full title: Structural analysis of the CD5 antigen

Authors: McAlister, M.; Brown, Marion H.; Willis, Antony C.; Rudd, Pauline M.; Harvey, David J.; Aplin, Robin T.; Shotton, David M.; Dwek, Raymond A.; Barclay, A. Neil; Driscoll, Paul C.

Year: 1998

Venue: European Journal of Biochemistry

DOI: https://doi.org/10.1046/j.1432-1327.1998.2570131.x

Paper Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice

Dalvie, Neil C.; Rodriguez‐Aponte, Sergio A.; Hartwell, Brittany L. et al. · 2021 · Proceedings of the National Academy of Sciences · DOI

Full title: Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice

Authors: Dalvie, Neil C.; Rodriguez‐Aponte, Sergio A.; Hartwell, Brittany L.; Tostanoski, Lisa H.; Biedermann, Andrew M.; Crowell, Laura E.; Kaur, Kawaljit; Kumru, Ozan S.; Carter, Lauren; Yu, Jingyou; Chang, Aiquan; McMahan, Katherine; Courant, Thomas; Lebas, Célia; Lemnios, Ashley A.; Rodrigues, Kristen A.; Silva, Murillo; Johnston, Ryan S.; Naranjo, Christopher A.; Tracey, Mary Kate; Brady, Joseph R.; Whittaker, Charles A.; Yun, Dong Soo; Brunette, Natalie; Wang, Jing Yang; Walkey, Carl; Fiala, Brooke; Kar, Swagata; Porto, Maciel; Lok, Megan; Andersen, Hanné; Lewis, Mark G.; Love, Kerry R.; Camp, Danielle L.; Silverman, Judith M.; Kleanthous, Harry; Joshi, Sangeeta B.; Volkin, David B.; Dubois, Patrice; Collin, Nicolas; King, Neil P.; Barouch, Dan H.; Irvine, Darrell J.; Love, J. Christopher

Year: 2021

Venue: Proceedings of the National Academy of Sciences

DOI: https://doi.org/10.1073/pnas.2106845118

Paper Established tools and emerging trends for the production of recombinant proteins and metabolites in Pichia pastoris.

Sonakshi De; D. Mattanovich; P. Ferrer et al. · 2021 · Essays in Biochemistry · DOI

Full title: Established tools and emerging trends for the production of recombinant proteins and metabolites in Pichia pastoris.

Authors: Sonakshi De; D. Mattanovich; P. Ferrer; Brigitte Gasser

Year: 2021

Venue: Essays in Biochemistry

DOI: https://doi.org/10.1042/ebc20200138

Paper Glycoengineering of antibody (Herceptin) through yeast expression and in vitro enzymatic glycosylation

Liu, Chiu-Ping; Tsai, Tsung-I; Cheng, Ting et al. · 2018 · Proceedings of the National Academy of Sciences · DOI

Full title: Glycoengineering of antibody (Herceptin) through yeast expression and in vitro enzymatic glycosylation

Authors: Liu, Chiu-Ping; Tsai, Tsung-I; Cheng, Ting; Shivatare, Vidya S.; Wu, Chung‐Yi; Wu, Chung‐Yi; Wong, Chi‐Huey

Year: 2018

Venue: Proceedings of the National Academy of Sciences

DOI: https://doi.org/10.1073/pnas.1718172115

Paper Mechanistic insights from plant heteromannan synthesis in yeast

Voiniciuc, Cătălin; Dama, Murali; Gawenda, Niklas et al. · 2018 · Proceedings of the National Academy of Sciences · DOI

Full title: Mechanistic insights from plant heteromannan synthesis in yeast

Authors: Voiniciuc, Cătălin; Dama, Murali; Gawenda, Niklas; Stritt, Fabian; Pauly, Markus

Year: 2018

Venue: Proceedings of the National Academy of Sciences

DOI: https://doi.org/10.1073/pnas.1814003116

Paper Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeastPichiapastoris

Choi, Byung‐Kwon; Bobrowicz, Piotr; Davidson, Robert C. et al. · 2003 · Proceedings of the National Academy of Sciences · DOI

Full title: Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeastPichiapastoris

Authors: Choi, Byung‐Kwon; Bobrowicz, Piotr; Davidson, Robert C.; Hamilton, Stephen R.; Kung, David H.; Li, Huijuan; Miele, Robert G.; Nett, Juergen H.; Wildt, Stefan; Gerngross, Tillman U.

Year: 2003

Venue: Proceedings of the National Academy of Sciences

DOI: https://doi.org/10.1073/pnas.0931263100

Paper In Mouse α-Methylacyl-CoA Racemase, the Same Gene Product Is Simultaneously Located in Mitochondria and Peroxisomes

Kotti, Tiina; Savolainen, Kalle; Helander, Heli et al. · 2000 · Journal of Biological Chemistry · DOI

Full title: In Mouse α-Methylacyl-CoA Racemase, the Same Gene Product Is Simultaneously Located in Mitochondria and Peroxisomes

Authors: Kotti, Tiina; Savolainen, Kalle; Helander, Heli; Yagi, Ahmed; Новиков, Д. К.; Kalkkinen, Nisse; Conzelmann, Ernst; Hiltunen, J. Kalervo; Schmitz, Werner

Year: 2000

Venue: Journal of Biological Chemistry

DOI: https://doi.org/10.1074/jbc.m002067200

Paper Direct evaluation of the effect of gene dosage on secretion of protein from yeast Pichia pastoris by expressing EGFP.

Hailong Liu; Yufeng Qin; Yuankai Huang et al. · 2014 · Journal of Microbiology and Biotechnology · DOI

Full title: Direct evaluation of the effect of gene dosage on secretion of protein from yeast Pichia pastoris by expressing EGFP.

Authors: Hailong Liu; Yufeng Qin; Yuankai Huang; Yaosheng Chen; P. Cong; Zuyong He

Year: 2014

Venue: Journal of Microbiology and Biotechnology

DOI: https://doi.org/10.4014/jmb.1308.08010

Paper Comparison of Two Laccases from Trametes versicolor for Application in the Decolorization of Dyes

Li, Qi; Ge, Lin; Cai, Junli et al. · 2014 · Journal of Microbiology and Biotechnology · DOI

Full title: Comparison of Two Laccases from Trametes versicolor for Application in the Decolorization of Dyes

Authors: Li, Qi; Ge, Lin; Cai, Junli; Pei, Jianjun; Xie, Jingcong; Zhao, Linguo

Year: 2014

Venue: Journal of Microbiology and Biotechnology

DOI: https://doi.org/10.4014/jmb.1310.10079

Paper Fungal Laccases: Production, Function, and Applications in Food Processing

Brijwani, Khushal; Rigdon, Anne R.; Vadlani, Praveen V. · 2010 · Enzyme Research · DOI

Full title: Fungal Laccases: Production, Function, and Applications in Food Processing

Authors: Brijwani, Khushal; Rigdon, Anne R.; Vadlani, Praveen V.

Year: 2010

Venue: Enzyme Research

DOI: https://doi.org/10.4061/2010/149748

Paper Constitutive expression of Botrytis aclada laccase in Pichia pastoris

R. Kittl; Christoph Gonaus; Christian Pillei et al. · 2012 · Bioengineered · DOI

Full title: Constitutive expression of Botrytis aclada laccase in Pichia pastoris

Authors: R. Kittl; Christoph Gonaus; Christian Pillei; D. Haltrich; R. Ludwig

Year: 2012

Venue: Bioengineered

DOI: https://doi.org/10.4161/bioe.20037

Paper Recombinant expression and characterization of an endoglucanase III (cel12a) from Trichoderma harzianum (Hypocreaceae) in the yeast Pichia pastoris

Generoso, Wesley Cardoso; Malagó-Jr, Wilson; Pereira, Nei et al. · 2012 · Genetics and Molecular Research · DOI

Full title: Recombinant expression and characterization of an endoglucanase III (cel12a) from Trichoderma harzianum (Hypocreaceae) in the yeast Pichia pastoris

Authors: Generoso, Wesley Cardoso; Malagó-Jr, Wilson; Pereira, Nei; Henrique‐Silva, Flávio

Year: 2012

Venue: Genetics and Molecular Research

DOI: https://doi.org/10.4238/2012.may.21.11

Paper Novel Strategies for Upstream and Downstream Processing of Tannin Acyl Hydrolase

Rodríguez‐Durán, Luis V.; Valdivia‐Urdiales, Blanca; Contreras‐Esquivel, Juan Carlos et al. · 2011 · Enzyme Research · DOI

Full title: Novel Strategies for Upstream and Downstream Processing of Tannin Acyl Hydrolase

Authors: Rodríguez‐Durán, Luis V.; Valdivia‐Urdiales, Blanca; Contreras‐Esquivel, Juan Carlos; Rodríguez‐Herrera, Raúl; Aguilar, Cristóbal N.

Year: 2011

Venue: Enzyme Research

DOI: https://doi.org/10.4061/2011/823619

Paper Advance in high-level heterologous expression of cyclodextrin glycosyltransferase

Li Xiao-han; Hao Jian-hua; Guo Jiao-Mei et al. · 2020

Full title: Advance in high-level heterologous expression of cyclodextrin glycosyltransferase

Authors: Li Xiao-han; Hao Jian-hua; Guo Jiao-Mei; Wang Wei; Sun Jing-jing; Liu Jun-zhong

Year: 2020

Paper Developing strategies for systems metabolic engineering of Pichia pastoris

Màrius Tomàs Gamisans · 2017

Full title: Developing strategies for systems metabolic engineering of Pichia pastoris

Authors: Màrius Tomàs Gamisans

Year: 2017

Paper Cloning and expression of single chain variable fragment of monoclonal antibody against norfloxacin in Pichia pastoris

J. Mala · DOI

Full title: Cloning and expression of single chain variable fragment of monoclonal antibody against norfloxacin in Pichia pastoris

Authors: J. Mala

DOI: https://doi.org/10.58837/chula.the.2015.909

Paper [Burkholderia cepacia lipase gene modification and its constitutive and inducible expression in Pichia pastoris].

Bin Jia; Wenshan Liu; Jiangke Yang et al. · 2010 · Wei sheng wu xue bao = Acta microbiologica Sinica

Full title: [Burkholderia cepacia lipase gene modification and its constitutive and inducible expression in Pichia pastoris].

Authors: Bin Jia; Wenshan Liu; Jiangke Yang; C. Ye; Li Xu; Yunjun Yan

Year: 2010

Venue: Wei sheng wu xue bao = Acta microbiologica Sinica

Paper A Step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches

Xavier Garcia Ortega · 2016

Full title: A Step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches

Authors: Xavier Garcia Ortega

Year: 2016

Paper [Cloning of a laccase gene from Flammulina velutipes and study on its expression in Pichia pastoris].

Yin-Bo Zhang; Q. Jiang; Mulan Jiang et al. · 2004 · Wei sheng wu xue bao = Acta microbiologica Sinica

Full title: [Cloning of a laccase gene from Flammulina velutipes and study on its expression in Pichia pastoris].

Authors: Yin-Bo Zhang; Q. Jiang; Mulan Jiang; Li-Xin Ma

Year: 2004

Venue: Wei sheng wu xue bao = Acta microbiologica Sinica

Paper Characterisation, cloning and production of industrially interesting enzymes : gluconolactone oxidase of Penicillium cyaneo-fulvum and gluconate 5-dehydrogenase of Gluconobacter suboxydans

Salusjärvi, Tuomas · 2006 · Työväentutkimus Vuosikirja

Full title: Characterisation, cloning and production of industrially interesting enzymes : gluconolactone oxidase of Penicillium cyaneo-fulvum and gluconate 5-dehydrogenase of Gluconobacter suboxydans

Authors: Salusjärvi, Tuomas

Year: 2006

Venue: Työväentutkimus Vuosikirja

Paper Different approaches to make Pichia pastoris a more versatile expression platform – process, strain and product engineering

D. Mattanovich; O. Spadiut; B. Eggenreich et al. · 2015

Full title: Different approaches to make Pichia pastoris a more versatile expression platform – process, strain and product engineering

Authors: D. Mattanovich; O. Spadiut; B. Eggenreich; D. Wurm; K. Metzger; Michaela Weissgram

Year: 2015

Paper Comparison of two codon optimization strategies enhancing recombinant Sus scrofa lysozyme production in Pichia pastoris.

D. Zhu; G. Cai; D. Wu et al. · 2015 · Cellular and Molecular Biology

Full title: Comparison of two codon optimization strategies enhancing recombinant Sus scrofa lysozyme production in Pichia pastoris.

Authors: D. Zhu; G. Cai; D. Wu; J. Lu

Year: 2015

Venue: Cellular and Molecular Biology

Paper Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris

Taype, Nieto; Ángel, Miguel · 2020 · TDX (Tesis Doctorals en Xarxa)

Full title: Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris

Authors: Taype, Nieto; Ángel, Miguel

Year: 2020

Venue: TDX (Tesis Doctorals en Xarxa)

Paper Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris

H. Le · 2019

Full title: Expression, purification, and characterization of recombinant basic fibroblast growth factor in pichia pastoris

Authors: H. Le

Year: 2019

Paper Dual Regulation of Cytoplasm and Peroxisomes for Improved Α-Farnesene Production in Recombinant Pichia pastoris.

Hui Liu; Shengmin Chen; Jian-zhong Xu et al. · 2021 · ACS Synthetic Biology · DOI

Full title: Dual Regulation of Cytoplasm and Peroxisomes for Improved Α-Farnesene Production in Recombinant Pichia pastoris.

Authors: Hui Liu; Shengmin Chen; Jian-zhong Xu; Weiguo Zhang

Year: 2021

Venue: ACS Synthetic Biology

DOI: https://doi.org/10.1021/acssynbio.1c00186

Paper Enhancing the expression of recombinant κ-carrageenase in Pichia pastoris using dual promoters, co-expressing chaperones and transcription factors

Yuan Yu; Zhemin Liu; Meng Chen et al. · 2020 · Biocatalysis and Biotransformation · DOI

Full title: Enhancing the expression of recombinant κ-carrageenase in Pichia pastoris using dual promoters, co-expressing chaperones and transcription factors

Authors: Yuan Yu; Zhemin Liu; Meng Chen; Min Yang; Li Li; H. Mou

Year: 2020

Venue: Biocatalysis and Biotransformation

DOI: https://doi.org/10.1080/10242422.2019.1655001

Thesis Effect of growth on protein production in a recombinant Pichia pastoris Mut+ strain in Fed-batch cultures

Balázs, Krisztina · 2016 · École polytechnique fédérale de Lausanne (EPFL) · DOI

Full title: Effect of growth on protein production in a recombinant Pichia pastoris Mut+ strain in Fed-batch cultures

Authors: Balázs, Krisztina

Year: 2016

Venue: École polytechnique fédérale de Lausanne (EPFL)

DOI: https://infoscience.epfl.ch/handle/20.500.14299/127063

Thesis A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris

Jungo, Carmen; Schenk, Jonas; Pasquier, Muriel et al. · 2007 · École polytechnique fédérale de Lausanne (EPFL) · DOI

Full title: A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris

Authors: Jungo, Carmen; Schenk, Jonas; Pasquier, Muriel; Marison, Ian; von Stockar, Urs

Year: 2007

Venue: École polytechnique fédérale de Lausanne (EPFL)

DOI: https://infoscience.epfl.ch/handle/20.500.14299/9713

Thesis Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B

Theron, Chrispian W; Vandermies, Marie; Telek, Samuel et al. · 2020 · University of Liverpool · DOI

Full title: Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B

Authors: Theron, Chrispian W; Vandermies, Marie; Telek, Samuel; Steels, Sebastien; Fickers, Patrick

Year: 2020

Venue: University of Liverpool

DOI: oai:livrepository.liverpool.ac.uk:3130242

Thesis Determination of the effects of fermentation conditions on the quality of protein expression in Pichia pastoris

Wegmann, Carole · 2007 · École polytechnique fédérale de Lausanne (EPFL) · DOI

Full title: Determination of the effects of fermentation conditions on the quality of protein expression in Pichia pastoris

Authors: Wegmann, Carole

Year: 2007

Venue: École polytechnique fédérale de Lausanne (EPFL)

DOI: https://infoscience.epfl.ch/handle/20.500.14299/8712

Thesis Quantitative characterization of the regulation of the synthesis of alcohol oxidase and of the expression of recombinant avidin in a Pichia pastoris Mut+ strain

Jungo, Carmen; Rérat, Claude; Marison, Ian et al. · 2007 · École polytechnique fédérale de Lausanne (EPFL) · DOI

Full title: Quantitative characterization of the regulation of the synthesis of alcohol oxidase and of the expression of recombinant avidin in a Pichia pastoris Mut+ strain

Authors: Jungo, Carmen; Rérat, Claude; Marison, Ian; von Stockar, Urs

Year: 2007

Venue: École polytechnique fédérale de Lausanne (EPFL)

DOI: https://infoscience.epfl.ch/handle/20.500.14299/239486

Thesis Optimisation of culture conditions with respect to biotin requirement for the production of recombinant avidin in Pichia pastoris

Jungo, C.; Urfer, J.; Zocchi, A. et al. · 2007 · École polytechnique fédérale de Lausanne (EPFL) · DOI

Full title: Optimisation of culture conditions with respect to biotin requirement for the production of recombinant avidin in Pichia pastoris

Authors: Jungo, C.; Urfer, J.; Zocchi, A.; Marison, I.; von Stockar, U.

Year: 2007

Venue: École polytechnique fédérale de Lausanne (EPFL)

DOI: https://infoscience.epfl.ch/handle/20.500.14299/239487

Thesis Production of protein‐based polymers in Pichia pastoris

Werten, Marc W.T. · 2017 · Wageningen University & Research · DOI

Full title: Production of protein‐based polymers in Pichia pastoris

Authors: Werten, Marc W.T.

Year: 2017

Venue: Wageningen University & Research

DOI: https://research.wur.nl/en/publications/production-of-proteinbased-polymers-in-pichia-pastoris

Thesis Pichia pastoris as a cell factory for the secreted production of tunable collagen-inspired gel-forming proteins

da Silva, C.I.F. · 2013 · Wageningen University & Research · DOI

Full title: Pichia pastoris as a cell factory for the secreted production of tunable collagen-inspired gel-forming proteins

Authors: da Silva, C.I.F.

Year: 2013

Venue: Wageningen University & Research

DOI: https://research.wur.nl/en/publications/pichia-pastoris-as-a-cell-factory-for-the-secreted-production-of-

Thesis Feeding strategies enhance high cell density cultivation and protein expression in milliliter scale bioreactors

Georg Faust, Nils H. Janzen, Christoph Bendig, Lin Römer, Klaus Kaufmann, Dirk Weuster-Botz · 2014 · Technical University of Munich (TUM) · DOI

Full title: Feeding strategies enhance high cell density cultivation and protein expression in milliliter scale bioreactors

Authors: Georg Faust, Nils H. Janzen, Christoph Bendig, Lin Römer, Klaus Kaufmann, Dirk Weuster-Botz

Year: 2014

Venue: Technical University of Munich (TUM)

DOI: https://mediatum.ub.tum.de/1231846

Thesis Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: high-level expression in Pichia pastoris and characterization of the recombinant protein

Berezina, Oksana V.; Herlet, Jonathan; Rykov, Sergey V. et al. · 2017 · Technical University of Munich (TUM) · DOI

Full title: Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: high-level expression in Pichia pastoris and characterization of the recombinant protein

Authors: Berezina, Oksana V.;Herlet, Jonathan;Rykov, Sergey V.;Kornberger, Petra;Zavyalov, Artem;Kozlov, Dmitriy;Sakhibgaraeva, Liliya;Krestyanova, Irina;Schwarz, Wolfgang H.;Zverlov, Vladimir V.;Liebl, Wolfgang;Yarotsky, Sergey V.

Year: 2017

Venue: Technical University of Munich (TUM)

DOI: https://mediatum.ub.tum.de/1422728

Thesis Advances in recombinant lipases: Production, engineering, immobilization and application in the pharmaceutical industry

Jares Contesini, Fabiano; Davanço, Marcelo Gomes; Borin, Gustavo Pagotto et al. · 2020 · Technical University of Denmark (DTU) · DOI

Full title: Advances in recombinant lipases: Production, engineering, immobilization and application in the pharmaceutical industry

Authors: Jares Contesini, Fabiano; Davanço, Marcelo Gomes; Borin, Gustavo Pagotto; Garcia Vanegas, Katherina; id_orcid 0000-0003-4407-5234; Cirino, João Pedro Gonçalves; de Melo, Ricardo Rodrigues; Mortensen, Uffe Hasbro; id_orcid 0000-0002-7794-7273; Hildén, Kristiina; Campos, Daniel Rossi; Carvalho, Patricia de Oliveira

Year: 2020

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/05ab7e5f-23cd-481c-b9b1-ea7a866ce814

Thesis Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data

Lund, Anne Mathilde; Kaas, Christian Schrøder; Brandl, Julian et al. · 2017 · Technical University of Denmark (DTU) · DOI

Full title: Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data

Authors: Lund, Anne Mathilde; Kaas, Christian Schrøder; Brandl, Julian; Pedersen, Lasse Ebdrup; id_orcid 0000-0002-6064-919X; Kildegaard, Helene Faustrup; Kristensen, Claus; Andersen, Mikael Rørdam; id_orcid 0000-0003-4794-6808

Year: 2017

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/1258a823-113f-4f97-bb1c-355d928cf36e

Thesis Model-assisted CRISPRi/a library screening reveals central carbon metabolic targets for enhanced recombinant protein production in yeast

Chen, Xin; Li, Feiran; Li, Xiaowei et al. · 2025 · Technical University of Denmark (DTU) · DOI

Full title: Model-assisted CRISPRi/a library screening reveals central carbon metabolic targets for enhanced recombinant protein production in yeast

Authors: Chen, Xin; Li, Feiran; Li, Xiaowei; Otto, Maximilian; Chen, Yu; Siewers, Verena

Year: 2025

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/1f4bb513-1a17-4a08-99c2-a87746baf059

Thesis Heterologous expression of hydrophobins RodA and RodB from Aspergillus fumigatus in host Pichia pastoris

Pedersen, Mona Højgaard; Borodina, Irina; Frisvad, Jens Christian et al. · 2009 · Technical University of Denmark (DTU) · DOI

Full title: Heterologous expression of hydrophobins RodA and RodB from Aspergillus fumigatus in host Pichia pastoris

Authors: Pedersen, Mona Højgaard; Borodina, Irina; Frisvad, Jens Christian; id_orcid 0000-0002-0573-4340; Søndergaard, Ib

Year: 2009

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/16071186-b9a5-4069-a7a7-df1af962087b

Thesis Genetic engineering approaches to improve post-translational modification of biopharmaceuticals in different production platforms

Amann, Thomas; Schmieder, Valerie; Kildegaard, Helene Faustrup et al. · 2019 · Technical University of Denmark (DTU) · DOI

Full title: Genetic engineering approaches to improve post-translational modification of biopharmaceuticals in different production platforms

Authors: Amann, Thomas; Schmieder, Valerie; Kildegaard, Helene Faustrup; Borth, Nicole; Andersen, Mikael Rørdam; id_orcid 0000-0003-4794-6808

Year: 2019

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/37ee779b-fc41-461c-9a0c-fa53ca39b38d

Thesis Balanced trafficking between the ER and the Golgi apparatus increases protein secretion in yeast

Bao, Jichen; Huang, Mingtao; Petranovic, Dina et al. · 2018 · Technical University of Denmark (DTU) · DOI

Full title: Balanced trafficking between the ER and the Golgi apparatus increases protein secretion in yeast

Authors: Bao, Jichen; Huang, Mingtao; Petranovic, Dina; Nielsen, Jens

Year: 2018

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/2fa50f5c-3d06-4289-b8c1-a799a58b40a0

Thesis Efficient protein production by yeast requires global tuning of metabolism

Huang, Mingtao; Bao, Jichen; Hallstrom, Bjorn M. et al. · 2017 · Technical University of Denmark (DTU) · DOI

Full title: Efficient protein production by yeast requires global tuning of metabolism

Authors: Huang, Mingtao; Bao, Jichen; Hallstrom, Bjorn M.; Petranovic, Dina; Nielsen, Jens

Year: 2017

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/4c51f4e5-c757-47a4-b6cb-15dc5c9824a5

Thesis Oligonucleotide-based CRISPR-Cas9 toolbox for efficient engineering of Komagataella phaffii

Strucko, Tomas; id_orcid 0000-0003-1947-8138; Gadar-Lopez, Adrian E. et al. · 2024 · Technical University of Denmark (DTU) · DOI

Full title: Oligonucleotide-based CRISPR-Cas9 toolbox for efficient engineering of Komagataella phaffii

Authors: Strucko, Tomas; id_orcid 0000-0003-1947-8138; Gadar-Lopez, Adrian E.; id_orcid 0000-0002-1608-1381; Frøhling, Frederik B.; Frost, Emma T.; Iversen, Esther F.; Olsson, Helen; Jarczynska, Zofia D.; id_orcid 0000-0001-6959-3260; Mortensen, Uffe H.; id_orcid 0000-0002-7794-7273

Year: 2024

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/2c8ee6ff-bf2e-489e-9f09-02d6b382665f

Thesis Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies

Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan et al. · 2022 · Technical University of Denmark (DTU) · DOI

Full title: Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies

Authors: Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan; Jensen, Michael Krogh; id_orcid 0000-0001-7574-4707; Workman, Christopher T.; id_orcid 0000-0002-2210-3743

Year: 2022

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/48772f7a-6c65-485d-acbd-89996481e14d

Thesis Non-conventional yeasts as superior production platforms for sustainable fermentation based bio-manufacturing processes

Navarrete, Clara; id_orcid 0000-0003-4958-0555; L. Martínez, José et al. · 2020 · Technical University of Denmark (DTU) · DOI

Full title: Non-conventional yeasts as superior production platforms for sustainable fermentation based bio-manufacturing processes

Authors: Navarrete, Clara; id_orcid 0000-0003-4958-0555; L. Martínez, José; id_orcid 0000-0002-4490-8534

Year: 2020

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/52df24dc-066e-4bd6-a47e-650edb535206

Thesis Monitoring and control of protein production in fungi

Schalén, Martin · 2015 · Technical University of Denmark (DTU) · DOI

Full title: Monitoring and control of protein production in fungi

Authors: Schalén, Martin

Year: 2015

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/86e1cdf4-c5d7-4b47-919b-992690edc74d

Thesis Characterizing heterologous protein burden in Komagataella phaffii

Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Jacobsen, Irene Hjorth et al. · 2025 · Technical University of Denmark (DTU) · DOI

Full title: Characterizing heterologous protein burden in Komagataella phaffii

Authors: Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Jacobsen, Irene Hjorth; Özdemir, Emre; id_orcid 0000-0001-5548-6720; Workman, Christopher T.; id_orcid 0000-0002-2210-3743; Jensen, Michael Krogh; id_orcid 0000-0001-7574-4707; Förster, Jochen; id_orcid 0000-0003-4045-7905

Year: 2025

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/78dfb490-e792-4a9f-87be-94193da6b781

Thesis In situ detection of protein interactions for recombinant therapeutic enzymes

Samoudi, Mojtaba; Chung Kuo, Chih; Robinson, Caressa M. et al. · 2021 · Technical University of Denmark (DTU) · DOI

Full title: In situ detection of protein interactions for recombinant therapeutic enzymes

Authors: Samoudi, Mojtaba; Chung Kuo, Chih; Robinson, Caressa M.; Shams-Ud-Doha, Km; Schinn, Song-Min; Kol, Stefan; Weiss, Linus; Petersen Bjørn, Sara; id_orcid 0000-0003-3341-6160; Voldborg, Bjørn G.; id_orcid 0000-0002-7005-1642; Rosa Campos, Alexandre; Lewis, Nathan E.

Year: 2021

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/a7368599-110a-4613-a99a-318f1f6b94e7

Thesis Engineered Reversal of Function in Glycolytic Yeast Promoters

Rajkumar, Arun S.; Özdemir, Emre; id_orcid 0000-0001-5548-6720 et al. · 2019 · Technical University of Denmark (DTU) · DOI

Full title: Engineered Reversal of Function in Glycolytic Yeast Promoters

Authors: Rajkumar, Arun S.; Özdemir, Emre; id_orcid 0000-0001-5548-6720; Lis, Alicia V.; Schneider, Konstantin; Qin, Jiufu; Jensen, Michael K.; id_orcid 0000-0001-7574-4707; Keasling, Jay D.

Year: 2019

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/b64425eb-99ac-45c3-b0ce-3ca871a4c4b9

Thesis Fungal cell with improved protein production capacity

Huang, Mingtao; Krivoruchko, Anastasia; David, Florian et al. · 2019 · Technical University of Denmark (DTU) · DOI

Full title: Fungal cell with improved protein production capacity

Authors: Huang, Mingtao; Krivoruchko, Anastasia; David, Florian; Nielsen, Jens

Year: 2019

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/cd0f1887-9c58-4260-9b57-c4e1a73e3e54

Thesis Expression of functional human sialyltransferases ST6GalNAc5 and ST6GalNAc6 in Pichia pastoris

Krasnoselska, Ganna; Lengyel, Marton; Matwiejuk, Martin et al. · 2025 · Technical University of Denmark (DTU) · DOI

Full title: Expression of functional human sialyltransferases ST6GalNAc5 and ST6GalNAc6 in Pichia pastoris

Authors: Krasnoselska, Ganna; Lengyel, Marton; Matwiejuk, Martin; Vuillemin, Marlene; Molnar-Gabor, Dora; Meyer, Anne S; id_orcid 0000-0001-8910-9931; Zeuner, Birgitte; id_orcid 0000-0002-8593-6742

Year: 2025

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/b964f139-ec97-4ba6-96db-65ed780e6fdc

Thesis A strategy for successful dual-species protein expression of genes with non-optimal codon usage destined for bacterial and yeast cell factories

Wäneskog, Marcus; Rasmussen, Trine Bertram; Jensen, Emil D. et al. · 2024 · Technical University of Denmark (DTU) · DOI

Full title: A strategy for successful dual-species protein expression of genes with non-optimal codon usage destined for bacterial and yeast cell factories

Authors: Wäneskog, Marcus; Rasmussen, Trine Bertram; Jensen, Emil D.; id_orcid 0000-0002-8280-0946

Year: 2024

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/c97b4925-3531-4348-aa7b-8b51c6913197

Thesis Expanding the molecular toolbox and understanding of Komagataella phaffii for optimised heterologous protein production

Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824 · 2024 · Technical University of Denmark (DTU) · DOI

Full title: Expanding the molecular toolbox and understanding of Komagataella phaffii for optimised heterologous protein production

Authors: Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824

Year: 2024

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/dd2cd38f-90f1-4fe6-bce8-aa77e6b701fc

Thesis Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Durmusoglu, Deniz; Al’Abri, Ibrahim; Li, Zidan et al. · 2023 · Technical University of Denmark (DTU) · DOI

Full title: Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Authors: Durmusoglu, Deniz; Al’Abri, Ibrahim; Li, Zidan; Islam Williams, Taufika; Collins, Leonard B.; Martínez, José L.; id_orcid 0000-0002-4490-8534; Crook, Nathan

Year: 2023

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/d66df130-4217-4e9e-b392-086c807ccb8e

Thesis Cell Factory Engineering

Davy, Anne Mathilde; Kildegaard, Helene Faustrup; Andersen, Mikael Rørdam et al. · 2017 · Technical University of Denmark (DTU) · DOI

Full title: Cell Factory Engineering

Authors: Davy, Anne Mathilde; Kildegaard, Helene Faustrup; Andersen, Mikael Rørdam; id_orcid 0000-0003-4794-6808

Year: 2017

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/f19f39eb-6cac-4674-b91f-f1b13b65fdb7

Thesis Customizing amino acid metabolism of Pichia pastoris for recombinant protein production

Rußmayer, Hannes; Buchetics, Markus; Mattanovich, Matthias et al. · 2023 · Technical University of Denmark (DTU) · DOI

Full title: Customizing amino acid metabolism of Pichia pastoris for recombinant protein production

Authors: Rußmayer, Hannes; Buchetics, Markus; Mattanovich, Matthias; Neubauer, Stefan; Steiger, Matthias; Graf, Alexandra B.; Koellensperger, Gunda; Hann, Stephan; Sauer, Michael; Gasser, Brigitte; Mattanovich, Diethard

Year: 2023

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/e65f1561-1cd7-495e-8db2-b64cc656c938

Thesis Codon-tRNA Coadaptation Bias for Identifying Strong Native Promoters in Komagataella phaffii

Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Petrov, Mykhaylo S. et al. · 2024 · Technical University of Denmark (DTU) · DOI

Full title: Codon-tRNA Coadaptation Bias for Identifying Strong Native Promoters in Komagataella phaffii

Authors: Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Petrov, Mykhaylo S.; Strucko, Tomas; id_orcid 0000-0003-1947-8138; Jensen, Michael K.; id_orcid 0000-0001-7574-4707; Workman, Christopher T.; id_orcid 0000-0002-2210-3743

Year: 2024

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/fcd9d993-6124-4c84-b09a-6a9c0c2ce7ee

Thesis High-level heterologous expression of active Chaetomium thermophilum FDH in Pichia pastoris

Duman, Zeynep Efsun; Duraksoy, Bedri Burak; Aktaş, Fatih et al. · 2020 · Technical University of Denmark (DTU) · DOI

Full title: High-level heterologous expression of active Chaetomium thermophilum FDH in Pichia pastoris

Authors: Duman, Zeynep Efsun; Duraksoy, Bedri Burak; Aktaş, Fatih; Woodley, John M.; id_orcid 0000-0002-7976-2483; Binay, Barış

Year: 2020

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/edbc2116-7c75-436f-96cd-abad094db112

Thesis Scale up study of monoclonal antibody fragment scFv production in recombinant Pichia pastoris with differen carbon source

Pessoa Junior, Adalberto · 2011 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Scale up study of monoclonal antibody fragment scFv production in recombinant Pichia pastoris with differen carbon source

Authors: Pessoa Junior, Adalberto

Year: 2011

Venue: Universidade de São Paulo – USP Digital Library

DOI: 002254622

Thesis Expression of recombinant mouse mast cell proteases 4 and 5 in Pichia pastoris

Jamur, Maria Celia · 2014 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Expression of recombinant mouse mast cell proteases 4 and 5 in Pichia pastoris

Authors: Jamur, Maria Celia

Year: 2014

Venue: Universidade de São Paulo – USP Digital Library

DOI: 002717378

Thesis Estudio del pH y la concentración de glicerol para la producción de antígeno recombinante de Plasmodium vivax usando Pichia pastoris

Pessoa Junior, Adalberto · 2015 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Estudio del pH y la concentración de glicerol para la producción de antígeno recombinante de Plasmodium vivax usando Pichia pastoris

Authors: Pessoa Junior, Adalberto

Year: 2015

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.reddeperuanos.com/revista/eci2015vrevista/index.html

Thesis Avaliation of the induction time of L-asparaginase II from Saccharomyces cerevisiae expressed in Pichia pastoris ('Mut POT. S')

Vitolo, Michele · 2015 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Avaliation of the induction time of L-asparaginase II from Saccharomyces cerevisiae expressed in Pichia pastoris ('Mut POT. S')

Authors: Vitolo, Michele

Year: 2015

Venue: Universidade de São Paulo – USP Digital Library

DOI: 002756062

Thesis Cultivation of Pichia pastoris carrying the scFv anti LDL (-) antibody fragment: effect of preculture carbon source

Pessoa Junior, Adalberto · 2017 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Cultivation of Pichia pastoris carrying the scFv anti LDL (-) antibody fragment: effect of preculture carbon source

Authors: Pessoa Junior, Adalberto

Year: 2017

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://doi.org/10.1016/j.bjm.2016.11.009

Thesis Free fatty acids reduce metabolic stress and favor a stable production of heterologous proteins in Pichia pastoris

Farías, Jorge G · 2018 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Free fatty acids reduce metabolic stress and favor a stable production of heterologous proteins in Pichia pastoris

Authors: Farías, Jorge G

Year: 2018

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://doi.org/10.1016/j.bjm.2018.03.008

Thesis Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast

Farías, Jorge G · 2018 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast

Authors: Farías, Jorge G

Year: 2018

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.bjmicrobiol.com.br/en/carbon-metabolism-influenced-for-promoters/avance-resumen/S1517838217306901/

Thesis Influence of carbon source on cell size and production of anti LDL (-) single-chain variable fragment by a recombinant Pichia pastoris strain

Converti, Attilio · 2019 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Influence of carbon source on cell size and production of anti LDL (-) single-chain variable fragment by a recombinant Pichia pastoris strain

Authors: Converti, Attilio

Year: 2019

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://doi.org/10.1007/s11033-019-04785-9

Thesis Cloning and expression of L-Asparaginase from Escherichia coli in a Pichia pastoris strain with humanized glycosylation

Monteiro, Gisele · 2018 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Cloning and expression of L-Asparaginase from Escherichia coli in a Pichia pastoris strain with humanized glycosylation

Authors: Monteiro, Gisele

Year: 2018

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.sbbq.org.br/reuniao/2018/livro_resumos_2018.pdf

Thesis Study of the Saccharomyces cerevisiae L-asparaginase 1 expression in Pichia pastoris glycoswitch strain): potential use in the treatment of acute lymphoblastic leukemia (LLA)

Monteiro, Gisele · 2018 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Study of the Saccharomyces cerevisiae L-asparaginase 1 expression in Pichia pastoris glycoswitch strain): potential use in the treatment of acute lymphoblastic leukemia (LLA)

Authors: Monteiro, Gisele

Year: 2018

Venue: Universidade de São Paulo – USP Digital Library

DOI: 002918975

Thesis Recombinant L-asparaginase production using Pichia pastoris (MUTs strain): establishment of conditions for growth and induction phases

Vitolo, Michele · 2021 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Recombinant L-asparaginase production using Pichia pastoris (MUTs strain): establishment of conditions for growth and induction phases

Authors: Vitolo, Michele

Year: 2021

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://doi.org/10.1002/jctb.6540

Thesis Increased glycosylated L-asparaginase production through selection of Pichia pastoris platform and oxygen-methanol control in fed-batches

Tonso, Aldo · 2021 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Increased glycosylated L-asparaginase production through selection of Pichia pastoris platform and oxygen-methanol control in fed-batches

Authors: Tonso, Aldo

Year: 2021

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://doi.org/10.1016/j.bej.2021.108083

Thesis Doubling humanized L-asparaginase expression by Pichia pastoris through DO-stat controlling induction strategy in bench-bioreactor

Tonso, Aldo · 2019 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Doubling humanized L-asparaginase expression by Pichia pastoris through DO-stat controlling induction strategy in bench-bioreactor

Authors: Tonso, Aldo

Year: 2019

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://www.aidic.it/ecce12/programma/1535parizotto.docx

Thesis Metabolic engineering of glycosylation and secretion pathways in Pichia pastoris and its application in production of the human recombinant enzyme N-acetylgalactosamine-6-sulfate sulfatase

Rodríguez López, Edwin Alexander · 2018 · Ghent University (Universiteit Gent) · DOI

Full title: Metabolic engineering of glycosylation and secretion pathways in Pichia pastoris and its application in production of the human recombinant enzyme N-acetylgalactosamine-6-sulfate sulfatase

Authors: Rodríguez López, Edwin Alexander

Year: 2018

Venue: Ghent University (Universiteit Gent)

DOI: https://biblio.ugent.be/publication/8577405

Thesis Yeast secretory system engineering for enhanced membrane protein expression.

Guerfal, Mouna · 2012 · Ghent University (Universiteit Gent) · DOI

Full title: Yeast secretory system engineering for enhanced membrane protein expression.

Authors: Guerfal, Mouna

Year: 2012

Venue: Ghent University (Universiteit Gent)

DOI: https://biblio.ugent.be/publication/4337090

Thesis Extracellular expression of Saccharomyces cerevisiae’s L-asparaginase II in Pichia pastoris results in novelenzyme with better parameters

Monteiro, Gisele · 2023 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Extracellular expression of Saccharomyces cerevisiae’s L-asparaginase II in Pichia pastoris results in novelenzyme with better parameters

Authors: Monteiro, Gisele

Year: 2023

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://doi.org/10.1080/10826068.2022.2111582

Thesis N-glycosylation engineering in Pichia pastoris for protein therapeutics: advancing the GlycoSwitch and introducing GlycoDelete

Laukens, Bram · 2016 · Ghent University (Universiteit Gent) · DOI

Full title: N-glycosylation engineering in Pichia pastoris for protein therapeutics: advancing the GlycoSwitch and introducing GlycoDelete

Authors: Laukens, Bram

Year: 2016

Venue: Ghent University (Universiteit Gent)

DOI: https://biblio.ugent.be/publication/8075923

Thesis Glycosylation and cell surface engineering in Pichia pastoris

Jacobs, Pieter P · 2008 · Ghent University (Universiteit Gent) · DOI

Full title: Glycosylation and cell surface engineering in Pichia pastoris

Authors: Jacobs, Pieter P

Year: 2008

Venue: Ghent University (Universiteit Gent)

DOI: https://biblio.ugent.be/publication/715147

Thesis Developing methods for the production of antiviral VHH-Fc antibodies in Pichia pastoris for fast pandemic response

Lonigro, Chiara · 2022 · Ghent University (Universiteit Gent) · DOI

Full title: Developing methods for the production of antiviral VHH-Fc antibodies in Pichia pastoris for fast pandemic response

Authors: Lonigro, Chiara

Year: 2022

Venue: Ghent University (Universiteit Gent)

DOI: https://biblio.ugent.be/publication/8744656

Thesis Engineering of the production of mannose-6-phosphate-modified lysosomal enzymes by the yeast Pichia pastoris

De Visscher, Charlotte · 2015 · Ghent University (Universiteit Gent) · DOI

Full title: Engineering of the production of mannose-6-phosphate-modified lysosomal enzymes by the yeast Pichia pastoris

Authors: De Visscher, Charlotte

Year: 2015

Venue: Ghent University (Universiteit Gent)

DOI: https://biblio.ugent.be/publication/5987332

Thesis Identifying Improved Sites for Heterologous Gene Integration Using ATAC-seq

Brady, Joseph R; Tan, Melody C; Whittaker, Charles A et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Identifying Improved Sites for Heterologous Gene Integration Using ATAC-seq

Authors: Brady, Joseph R; Tan, Melody C; Whittaker, Charles A; Colant, Noelle A; Dalvie, Neil C; Love, Kerry Routenberg; Love, J Christopher

Year: 2021

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/134411

Thesis Identifying Improved Sites for Heterologous Gene Integration Using ATAC-seq

Brady, Joseph R.; Tan, Melody C.; Whittaker, Charles A. et al. · 2022 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Identifying Improved Sites for Heterologous Gene Integration Using ATAC-seq

Authors: Brady, Joseph R.; Tan, Melody C.; Whittaker, Charles A.; Colant, Noelle A.; Dalvie, Neil C.; Love, Kerry Routenberg; Love, J. Christopher

Year: 2022

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/134411.2

Thesis Challenging the workhorse: Comparative analysis of eukaryotic micro‐organisms for expressing monoclonal antibodies

Jiang, Hanxiao; Horwitz, Andrew A; Wright, Chapman et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Challenging the workhorse: Comparative analysis of eukaryotic micro‐organisms for expressing monoclonal antibodies

Authors: Jiang, Hanxiao; Horwitz, Andrew A; Wright, Chapman; Tai, Anna; Znameroski, Elizabeth A; Tsegaye, Yoseph; Warbington, Hailley; Bower, Benjamin S; Alves, Christina; Co, Carl; Jonnalagadda, Kanvasri; Platt, Darren; Walter, Jessica M; Natarajan, Venkatesh; Ubersax, Jeffrey A; Cherry, Joel R; Love, J Christopher

Year: 2021

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/134784

Thesis Expression and purification of a recombinant avidin with a lowered isoelectric point in Pichia pastoris

Zocchi, Andrea; Marya Jobe, Anna; Neuhaus, Jean-Marc et al. · 2025 · University of Basel - edoc Repository · DOI

Full title: Expression and purification of a recombinant avidin with a lowered isoelectric point in Pichia pastoris

Authors: Zocchi, Andrea; Marya Jobe, Anna; Neuhaus, Jean-Marc; Ward, Thomas R.

Year: 2025

Venue: University of Basel - edoc Repository

DOI: http://hdl.handle.net/20.500.14716/67275

Thesis Improving the expression of UPOs using signal peptide domain engineering

Camboni, Ginevra · 2023 · White Rose eTheses (Leeds, Sheffield, York) · DOI

Full title: Improving the expression of UPOs using signal peptide domain engineering

Authors: Camboni, Ginevra

Year: 2023

Venue: White Rose eTheses (Leeds, Sheffield, York)

DOI: oai:etheses.whiterose.ac.uk:34876

Thesis Caractérisation quantitative de la croissance et du système d’expression d’une souche recombinante de Pichia pastoris Mut+: influence du taux de dilution et du type de substrat

Rérat, Claude · 2007 · École polytechnique fédérale de Lausanne (EPFL) · DOI

Full title: Caractérisation quantitative de la croissance et du système d’expression d’une souche recombinante de Pichia pastoris Mut+: influence du taux de dilution et du type de substrat

Authors: Rérat, Claude

Year: 2007

Venue: École polytechnique fédérale de Lausanne (EPFL)

DOI: https://infoscience.epfl.ch/handle/20.500.14299/8678

Thesis Optimisation de la production d'avidine sécrétée par une lignée mut+ de P. pastoris en culture fed-batch à une haute densité cellulaire

Pasquier, Muriel · 2007 · École polytechnique fédérale de Lausanne (EPFL) · DOI

Full title: Optimisation de la production d'avidine sécrétée par une lignée mut+ de P. pastoris en culture fed-batch à une haute densité cellulaire

Authors: Pasquier, Muriel

Year: 2007

Venue: École polytechnique fédérale de Lausanne (EPFL)

DOI: https://infoscience.epfl.ch/handle/20.500.14299/8706

Thesis Influence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut(+) strain

Schenk, Jonas; Balazs, Krisztina; Jungo, Carmen et al. · 2010 · École polytechnique fédérale de Lausanne (EPFL) · DOI

Full title: Influence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut(+) strain

Authors: Schenk, Jonas; Balazs, Krisztina; Jungo, Carmen; Urfer, Julien; Wegmann, Carole; Zocchi, Andrea; Marison, Ian W.; von Stockar, Urs

Year: 2010

Venue: École polytechnique fédérale de Lausanne (EPFL)

DOI: https://infoscience.epfl.ch/handle/20.500.14299/58381

Thesis Model-assisted CRISPRi/a library screening reveals central carbon metabolic targets for enhanced recombinant protein production in yeast

Chen, Xin; Li, Feiran; Li, Xiaowei et al. · 2025 · Technical University of Denmark (DTU) · DOI

Full title: Model-assisted CRISPRi/a library screening reveals central carbon metabolic targets for enhanced recombinant protein production in yeast

Authors: Chen, Xin; Li, Feiran; Li, Xiaowei; Otto, Maximilian; Chen, Yu; Siewers, Verena

Year: 2025

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/1f4bb513-1a17-4a08-99c2-a87746baf059

Thesis Heterologous expression of hydrophobins RodA and RodB from Aspergillus fumigatus in host Pichia pastoris

Pedersen, Mona Højgaard; Borodina, Irina; Frisvad, Jens Christian et al. · 2009 · Technical University of Denmark (DTU) · DOI

Full title: Heterologous expression of hydrophobins RodA and RodB from Aspergillus fumigatus in host Pichia pastoris

Authors: Pedersen, Mona Højgaard; Borodina, Irina; Frisvad, Jens Christian; id_orcid 0000-0002-0573-4340; Søndergaard, Ib

Year: 2009

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/16071186-b9a5-4069-a7a7-df1af962087b

Thesis Advances in recombinant lipases: Production, engineering, immobilization and application in the pharmaceutical industry

Jares Contesini, Fabiano; Davanço, Marcelo Gomes; Borin, Gustavo Pagotto et al. · 2020 · Technical University of Denmark (DTU) · DOI

Full title: Advances in recombinant lipases: Production, engineering, immobilization and application in the pharmaceutical industry

Year: 2020

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/05ab7e5f-23cd-481c-b9b1-ea7a866ce814

Thesis Moderate expression of SEC16 increases protein secretion by Saccharomyces cerevisiae

Bao, Jichen; Huang, Mingtao; Petranovic, Dina et al. · 2017 · Technical University of Denmark (DTU) · DOI

Full title: Moderate expression of SEC16 increases protein secretion by Saccharomyces cerevisiae

Authors: Bao, Jichen; Huang, Mingtao; Petranovic, Dina; Nielsen, Jens

Year: 2017

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/33c5dfcd-beb8-41e7-a5c3-c190136e2cbd

Thesis Biopharmaceutical protein production bySaccharomyces cerevisiae: current state and future prospects

Huang, Mingtao; Bao, Jichen; Nielsen, Jens · 2014 · Technical University of Denmark (DTU) · DOI

Full title: Biopharmaceutical protein production bySaccharomyces cerevisiae: current state and future prospects

Authors: Huang, Mingtao; Bao, Jichen; Nielsen, Jens

Year: 2014

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/29c6e70b-6a1d-43df-bf59-82bd66ed6cca

Thesis P. pastoris (K. phaffi) perfusion processes: evaluation of cell retention techniques and integrated purification strategies

Linova, M.; id_orcid 0000-0001-7716-1257; Kodiripaka, S. et al. · 2025 · Technical University of Denmark (DTU) · DOI

Full title: P. pastoris (K. phaffi) perfusion processes: evaluation of cell retention techniques and integrated purification strategies

Authors: Linova, M.; id_orcid 0000-0001-7716-1257; Kodiripaka, S.; Martins, E.; Pham, D.; id_orcid 0000-0002-7804-3882; Sripada, S.; Menegatti, S.; Carbonell, R.; Woodley, J.; id_orcid 0000-0002-7976-2483

Year: 2025

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/2d3b56e0-24f0-449e-bda9-fd8ea452eaaf

Thesis Oligonucleotide-based CRISPR-Cas9 toolbox for efficient engineering of Komagataella phaffii

Strucko, Tomas; id_orcid 0000-0003-1947-8138; Gadar-Lopez, Adrian E. et al. · 2024 · Technical University of Denmark (DTU) · DOI

Full title: Oligonucleotide-based CRISPR-Cas9 toolbox for efficient engineering of Komagataella phaffii

Year: 2024

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/2c8ee6ff-bf2e-489e-9f09-02d6b382665f

Thesis Application of native signal sequences for recombinant proteins secretion in Pichia pastoris

Borodina, Irina; Do, Duy Duc; Eriksen, Jens C. et al. · 2011 · Technical University of Denmark (DTU) · DOI

Full title: Application of native signal sequences for recombinant proteins secretion in Pichia pastoris

Authors: Borodina, Irina; Do, Duy Duc; Eriksen, Jens C.; Strucko, Tomas; id_orcid 0000-0003-1947-8138; Mortensen, Uffe Hasbro; id_orcid 0000-0002-7794-7273; Eliasson Lantz, Anna

Year: 2011

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/4945b5ae-73ed-4110-b7da-b5f83b5f67da

Thesis Improved Production of a Heterologous Amylase in Saccharomyces cerevisiae by Inverse Metabolic Engineering

Liu, Zihe; Liu, Lifang; Osterlund, Tobias et al. · 2014 · Technical University of Denmark (DTU) · DOI

Full title: Improved Production of a Heterologous Amylase in Saccharomyces cerevisiae by Inverse Metabolic Engineering

Authors: Liu, Zihe; Liu, Lifang; Osterlund, Tobias; Hou, Jin; Huang, Mingtao; Fagerberg, Linn; Petranovic, Dina; Uhlén, Mathias; Nielsen, Jens

Year: 2014

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/5468babc-d23d-4dd8-96b0-fcc3f8ddf2fa

Thesis Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies

Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan et al. · 2022 · Technical University of Denmark (DTU) · DOI

Full title: Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies

Authors: Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan; Jensen, Michael Krogh; id_orcid 0000-0001-7574-4707; Workman, Christopher T.; id_orcid 0000-0002-2210-3743

Year: 2022

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/48772f7a-6c65-485d-acbd-89996481e14d

Thesis Genome-scale metabolic model of Pichia pastoris with native and humanized glycosylation of recombinant proteins

Irani, Zahra Azimzadeh; Kerkhoven, Eduard J.; Shojaosadati, Seyed Abbas et al. · 2016 · Technical University of Denmark (DTU) · DOI

Full title: Genome-scale metabolic model of Pichia pastoris with native and humanized glycosylation of recombinant proteins

Authors: Irani, Zahra Azimzadeh; Kerkhoven, Eduard J.; Shojaosadati, Seyed Abbas; Nielsen, Jens

Year: 2016

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/33e2d41c-b61a-40d7-befe-f48d7a160ca3

Thesis Non-conventional yeasts as superior production platforms for sustainable fermentation based bio-manufacturing processes

Navarrete, Clara; id_orcid 0000-0003-4958-0555; L. Martínez, José et al. · 2020 · Technical University of Denmark (DTU) · DOI

Full title: Non-conventional yeasts as superior production platforms for sustainable fermentation based bio-manufacturing processes

Authors: Navarrete, Clara; id_orcid 0000-0003-4958-0555; L. Martínez, José; id_orcid 0000-0002-4490-8534

Year: 2020

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/52df24dc-066e-4bd6-a47e-650edb535206

Thesis Efficient protein production by yeast requires global tuning of metabolism

Huang, Mingtao; Bao, Jichen; Hallstrom, Bjorn M. et al. · 2017 · Technical University of Denmark (DTU) · DOI

Full title: Efficient protein production by yeast requires global tuning of metabolism

Authors: Huang, Mingtao; Bao, Jichen; Hallstrom, Bjorn M.; Petranovic, Dina; Nielsen, Jens

Year: 2017

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/4c51f4e5-c757-47a4-b6cb-15dc5c9824a5

Thesis Genetic engineering approaches to improve post-translational modification of biopharmaceuticals in different production platforms

Amann, Thomas; Schmieder, Valerie; Kildegaard, Helene Faustrup et al. · 2019 · Technical University of Denmark (DTU) · DOI

Full title: Genetic engineering approaches to improve post-translational modification of biopharmaceuticals in different production platforms

Authors: Amann, Thomas; Schmieder, Valerie; Kildegaard, Helene Faustrup; Borth, Nicole; Andersen, Mikael Rørdam; id_orcid 0000-0003-4794-6808

Year: 2019

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/37ee779b-fc41-461c-9a0c-fa53ca39b38d

Thesis CRISPR/Cas9 advances engineering of microbial cell factories

Jakociunas, Tadas; id_orcid 0000-0003-1264-173X; Jensen, Michael Krogh et al. · 2016 · Technical University of Denmark (DTU) · DOI

Full title: CRISPR/Cas9 advances engineering of microbial cell factories

Authors: Jakociunas, Tadas; id_orcid 0000-0003-1264-173X; Jensen, Michael Krogh; id_orcid 0000-0001-7574-4707; Keasling, Jay D.

Year: 2016

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/553afcd1-ceb8-4708-bf9d-f4a0952fe9af

Thesis Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data

Lund, Anne Mathilde; Kaas, Christian Schrøder; Brandl, Julian et al. · 2017 · University of Copenhagen · DOI

Full title: Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data

Authors: Lund, Anne Mathilde; Kaas, Christian Schrøder; Brandl, Julian; Pedersen, Lasse Ebdrup; Kildegaard, Helene Faustrup; Kristensen, Claus; Andersen, Mikael Rørdam

Year: 2017

Venue: University of Copenhagen

DOI: https://researchprofiles.ku.dk/da/publications/7a48f042-480d-4c8d-a3ea-c66a9d53ba97

Thesis Fed-batch production of the hydrophobins RodA and RodB from Aspergillus fumigatus in host Pichia pastoris

Pedersen, Mona Højgaard; Borodina, Irina; Frisvad, Jens Christian et al. · 2011 · Technical University of Denmark (DTU) · DOI

Full title: Fed-batch production of the hydrophobins RodA and RodB from Aspergillus fumigatus in host Pichia pastoris

Authors: Pedersen, Mona Højgaard; Borodina, Irina; Frisvad, Jens Christian; id_orcid 0000-0002-0573-4340; Søndergaard, Ib

Year: 2011

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/67337f0c-b465-4be1-8fb6-4b32cb6311c5

Thesis Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast

Huang, Mingtao; Bai, Yunpeng; Sjostrom, Staffan L. et al. · 2015 · Technical University of Denmark (DTU) · DOI

Full title: Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast

Authors: Huang, Mingtao; Bai, Yunpeng; Sjostrom, Staffan L.; Hallström, Björn M.; Liu, Zihe; Petranovic, Dina; Uhlén, Mathias; Joensson, Haakan N.; Svahn, Helene Andersson; Nielsen, Jens

Year: 2015

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/7610d98c-da78-4f49-8920-181c255ffbbd

Thesis Characterizing heterologous protein burden in Komagataella phaffii

Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Jacobsen, Irene Hjorth et al. · 2025 · Technical University of Denmark (DTU) · DOI

Full title: Characterizing heterologous protein burden in Komagataella phaffii

Year: 2025

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/78dfb490-e792-4a9f-87be-94193da6b781

Thesis Production in Pichia pastoris and characterization of recombinant barley limit dextrinase

Vester-Christensen, Malene Bech; Næsted, Henrik; Abou Hachem, Maher et al. · 2008 · Technical University of Denmark (DTU) · DOI

Full title: Production in Pichia pastoris and characterization of recombinant barley limit dextrinase

Authors: Vester-Christensen, Malene Bech; Næsted, Henrik; Abou Hachem, Maher; id_orcid 0000-0001-8250-1842; Svensson, Birte; id_orcid 0000-0002-2993-8196

Year: 2008

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/8e760720-483f-4da4-bbe8-b5c36f86d43e

Thesis Expression of antibody fragments in Saccharomyces cerevisiae strains evolved for enhanced protein secretion

Wang, Yanyan; Li, Xiaowei; Chen, Xin et al. · 2021 · Technical University of Denmark (DTU) · DOI

Full title: Expression of antibody fragments in Saccharomyces cerevisiae strains evolved for enhanced protein secretion

Authors: Wang, Yanyan; Li, Xiaowei; Chen, Xin; Nielsen, Jens; Petranovic, Dina; Siewers, Verena

Year: 2021

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/7f70d6dd-3490-4db7-be9b-c8be2d8f8e8f

Thesis Production of enzymatically active recombinant full-length barley high pI alpha-glucosidase of glycoside family 31 by high cell-density fermentation of Pichia pastoris and affinity purification

Næsted, Henrik; Kramhøft, Birte; Lok, F. et al. · 2006 · Technical University of Denmark (DTU) · DOI

Full title: Production of enzymatically active recombinant full-length barley high pI alpha-glucosidase of glycoside family 31 by high cell-density fermentation of Pichia pastoris and affinity purification

Authors: Næsted, Henrik; Kramhøft, Birte; Lok, F.; Bojsen, K.; Yu, S.; Svensson, Birte; id_orcid 0000-0002-2993-8196

Year: 2006

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/8cd709dc-7b62-48ca-b624-48f926f19985

Thesis In situ detection of protein interactions for recombinant therapeutic enzymes

Samoudi, Mojtaba; Chung Kuo, Chih; Robinson, Caressa M. et al. · 2021 · Technical University of Denmark (DTU) · DOI

Full title: In situ detection of protein interactions for recombinant therapeutic enzymes

Year: 2021

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/a7368599-110a-4613-a99a-318f1f6b94e7

Thesis Efficient secretory expression of functional barley limit dextrinase inhibitor by high cell-density fermentation of Pichia pastoris

Jensen, Johanne Mørch; Vester-Christensen, Malene Bech; Møller, Marie Sofie et al. · 2011 · Technical University of Denmark (DTU) · DOI

Full title: Efficient secretory expression of functional barley limit dextrinase inhibitor by high cell-density fermentation of Pichia pastoris

Authors: Jensen, Johanne Mørch; Vester-Christensen, Malene Bech; Møller, Marie Sofie; id_orcid 0000-0001-9017-3367; Bønsager, Birgit Christine; Christensen, Hans Erik Mølager; Abou Hachem, Maher; id_orcid 0000-0001-8250-1842; Svensson, Birte; id_orcid 0000-0002-2993-8196

Year: 2011

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/ab6f5acb-ae51-4851-925e-924b21981453

Thesis High-yield production of hydrophobins RodA and RodB from Aspergillus fumigatus in Pichia pastoris

Pedersen, Mona Højgaard; Borodina, Irina; Moresco, Jacob Lange et al. · 2011 · Technical University of Denmark (DTU) · DOI

Full title: High-yield production of hydrophobins RodA and RodB from Aspergillus fumigatus in Pichia pastoris

Authors: Pedersen, Mona Højgaard; Borodina, Irina; Moresco, Jacob Lange; Svendsen, Winnie Edith; id_orcid 0000-0002-9197-6505; Frisvad, Jens Christian; id_orcid 0000-0002-0573-4340; Søndergaard, Ib

Year: 2011

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/a1ccacd9-c34f-4da7-8544-b884e71f635e

Thesis Expression of functional human sialyltransferases ST6GalNAc5 and ST6GalNAc6 in Pichia pastoris

Krasnoselska, Ganna; Lengyel, Marton; Matwiejuk, Martin et al. · 2025 · Technical University of Denmark (DTU) · DOI

Full title: Expression of functional human sialyltransferases ST6GalNAc5 and ST6GalNAc6 in Pichia pastoris

Authors: Krasnoselska, Ganna; Lengyel, Marton; Matwiejuk, Martin; Vuillemin, Marlene; Molnar-Gabor, Dora; Meyer, Anne S; id_orcid 0000-0001-8910-9931; Zeuner, Birgitte; id_orcid 0000-0002-8593-6742

Year: 2025

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/b964f139-ec97-4ba6-96db-65ed780e6fdc

Thesis Fungal cell with improved protein production capacity

Huang, Mingtao; Krivoruchko, Anastasia; David, Florian et al. · 2019 · Technical University of Denmark (DTU) · DOI

Full title: Fungal cell with improved protein production capacity

Authors: Huang, Mingtao; Krivoruchko, Anastasia; David, Florian; Nielsen, Jens

Year: 2019

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/cd0f1887-9c58-4260-9b57-c4e1a73e3e54

Thesis Engineered Reversal of Function in Glycolytic Yeast Promoters

Rajkumar, Arun S.; Özdemir, Emre; id_orcid 0000-0001-5548-6720 et al. · 2019 · Technical University of Denmark (DTU) · DOI

Full title: Engineered Reversal of Function in Glycolytic Yeast Promoters

Authors: Rajkumar, Arun S.; Özdemir, Emre; id_orcid 0000-0001-5548-6720; Lis, Alicia V.; Schneider, Konstantin; Qin, Jiufu; Jensen, Michael K.; id_orcid 0000-0001-7574-4707; Keasling, Jay D.

Year: 2019

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/b64425eb-99ac-45c3-b0ce-3ca871a4c4b9

Thesis Novel and improved yeast cell factories for biosustainable processes

Workman, Mhairi · 2014 · Technical University of Denmark (DTU) · DOI

Full title: Novel and improved yeast cell factories for biosustainable processes

Authors: Workman, Mhairi

Year: 2014

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/cb974a12-ee35-4d5d-926e-654c2e0c5405

Thesis Expanding the molecular toolbox and understanding of Komagataella phaffii for optimised heterologous protein production

Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824 · 2024 · Technical University of Denmark (DTU) · DOI

Full title: Expanding the molecular toolbox and understanding of Komagataella phaffii for optimised heterologous protein production

Authors: Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824

Year: 2024

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/dd2cd38f-90f1-4fe6-bce8-aa77e6b701fc

Thesis Novel multimodal cation-exchange membrane for the purification of a single-chain variable fragment from Pichia pastoris supernatant

Pham, Dan N.; id_orcid 0000-0002-7804-3882; Linova, Marina Y. et al. · 2024 · Technical University of Denmark (DTU) · DOI

Full title: Novel multimodal cation-exchange membrane for the purification of a single-chain variable fragment from Pichia pastoris supernatant

Authors: Pham, Dan N.; id_orcid 0000-0002-7804-3882; Linova, Marina Y.; id_orcid 0000-0001-7716-1257; Smith, William K.; Brown, Hunter; Elhanafi, Driss; Fan, Jinxin; Lavoie, Joseph; Woodley, John M.; id_orcid 0000-0002-7976-2483; Carbonell, Ruben G.

Year: 2024

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/d389f2ef-aa9b-44aa-ab2a-6cc339b6945f

Thesis Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Durmusoglu, Deniz; Al’Abri, Ibrahim; Li, Zidan et al. · 2023 · Technical University of Denmark (DTU) · DOI

Full title: Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Authors: Durmusoglu, Deniz; Al’Abri, Ibrahim; Li, Zidan; Islam Williams, Taufika; Collins, Leonard B.; Martínez, José L.; id_orcid 0000-0002-4490-8534; Crook, Nathan

Year: 2023

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/d66df130-4217-4e9e-b392-086c807ccb8e

Thesis Customizing amino acid metabolism of Pichia pastoris for recombinant protein production

Rußmayer, Hannes; Buchetics, Markus; Mattanovich, Matthias et al. · 2023 · Technical University of Denmark (DTU) · DOI

Full title: Customizing amino acid metabolism of Pichia pastoris for recombinant protein production

Year: 2023

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/e65f1561-1cd7-495e-8db2-b64cc656c938

Thesis Fed-batch production of hydrophobin RodB from Aspergillus fumigatus in host Pichia pastoris

Pedersen, Mona Højgaard; Borodina, Irina; Frisvad, Jens Christian et al. · 2011 · Technical University of Denmark (DTU) · DOI

Full title: Fed-batch production of hydrophobin RodB from Aspergillus fumigatus in host Pichia pastoris

Authors: Pedersen, Mona Højgaard; Borodina, Irina; Frisvad, Jens Christian; id_orcid 0000-0002-0573-4340; Søndergaard, Ib

Year: 2011

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/da6e84be-4add-4c73-a4a8-467d890612a3

Thesis Production in Pichia pastoris and characterization of recombinant barley limit dextrinase

Vester-Christensen, Malene Bech; Næsted, Henrik; Abou Hachem, Maher et al. · 2008 · Technical University of Denmark (DTU) · DOI

Full title: Production in Pichia pastoris and characterization of recombinant barley limit dextrinase

Authors: Vester-Christensen, Malene Bech; Næsted, Henrik; Abou Hachem, Maher; id_orcid 0000-0001-8250-1842; Svensson, Birte; id_orcid 0000-0002-2993-8196

Year: 2008

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/e6a884f2-5698-4cb8-adfd-e92629370bf5

Thesis High-level expression, secretion, and purification of the thermostable aqualysin I from Thermus aquaticus YT-1 in Pichia pastoris

Oledzka, G.; Dabrowski, Slawomir; Kur, J. · 2003 · Technical University of Denmark (DTU) · DOI

Full title: High-level expression, secretion, and purification of the thermostable aqualysin I from Thermus aquaticus YT-1 in Pichia pastoris

Authors: Oledzka, G.; Dabrowski, Slawomir; Kur, J.

Year: 2003

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/e1399fb4-8a41-4cd2-95bf-a4e6d83777a4

Thesis Using Pichia pastoris to produce recombinant glycerol kinase

Terrazas, Werner Damião Morhy; Aizemberg, Raquel [UNESP]; Gattas, Edwil Aparecida de Lucca [UNESP] · 2016 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Using Pichia pastoris to produce recombinant glycerol kinase

Authors: Terrazas, Werner Damião Morhy; Aizemberg, Raquel [UNESP]; Gattas, Edwil Aparecida de Lucca [UNESP]

Year: 2016

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/3143

Thesis High-level heterologous expression of active Chaetomium thermophilum FDH in Pichia pastoris

Duman, Zeynep Efsun; Duraksoy, Bedri Burak; Aktaş, Fatih et al. · 2020 · Technical University of Denmark (DTU) · DOI

Full title: High-level heterologous expression of active Chaetomium thermophilum FDH in Pichia pastoris

Authors: Duman, Zeynep Efsun; Duraksoy, Bedri Burak; Aktaş, Fatih; Woodley, John M.; id_orcid 0000-0002-7976-2483; Binay, Barış

Year: 2020

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/edbc2116-7c75-436f-96cd-abad094db112

Thesis Produção de glycerol quinase recombinante utilizando o sistema Pichia pastoris

Terrazas, Werner Damião Morhy; Aizemberg, Raquel [UNESP]; Gattás, Edwil Aparecida de Lucca [UNESP] · 2018 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Produção de glycerol quinase recombinante utilizando o sistema Pichia pastoris

Authors: Terrazas, Werner Damião Morhy; Aizemberg, Raquel [UNESP]; Gattás, Edwil Aparecida de Lucca [UNESP]

Year: 2018

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://hdl.handle.net/11449/177286

Thesis Codon-tRNA Coadaptation Bias for Identifying Strong Native Promoters in Komagataella phaffii

Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Petrov, Mykhaylo S. et al. · 2024 · Technical University of Denmark (DTU) · DOI

Full title: Codon-tRNA Coadaptation Bias for Identifying Strong Native Promoters in Komagataella phaffii

Year: 2024

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/fcd9d993-6124-4c84-b09a-6a9c0c2ce7ee

Thesis Expression and processing of recombinant sarafotoxins precursor in Pichia pastoris

Borgheresi, RAMB; Palma, Mario Sergio [UNESP]; Ducancel, F. et al. · 2014 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Expression and processing of recombinant sarafotoxins precursor in Pichia pastoris

Authors: Borgheresi, RAMB; Palma, Mario Sergio [UNESP]; Ducancel, F.; Camargo, ACM; Carmona, E.

Year: 2014

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://dx.doi.org/10.1016/S0041-0101(00)00265-8

Thesis Cell Factory Engineering

Davy, Anne Mathilde; Kildegaard, Helene Faustrup; Andersen, Mikael Rørdam et al. · 2017 · Technical University of Denmark (DTU) · DOI

Full title: Cell Factory Engineering

Authors: Davy, Anne Mathilde; Kildegaard, Helene Faustrup; Andersen, Mikael Rørdam; id_orcid 0000-0003-4794-6808

Year: 2017

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/f19f39eb-6cac-4674-b91f-f1b13b65fdb7

Thesis Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review

Silva, Viviane C. [UNESP]; Peres, Maristela F. S. [UNESP]; Gattás, Edwil A. L. [UNESP] · 2022 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review

Authors: Silva, Viviane C. [UNESP]; Peres, Maristela F. S. [UNESP]; Gattás, Edwil A. L. [UNESP]

Year: 2022

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://hdl.handle.net/11449/225485

Thesis Recombinant expression of glycerol-3-phosphate dehydrogenase using the Pichia pastoris system

Sanches Peres, Maristela de Freitas [UNESP]; Silva, Viviane Cristina [UNESP]; Valentini, Sandro Roberto [UNESP] et al. · 2014 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Recombinant expression of glycerol-3-phosphate dehydrogenase using the Pichia pastoris system

Authors: Sanches Peres, Maristela de Freitas [UNESP]; Silva, Viviane Cristina [UNESP]; Valentini, Sandro Roberto [UNESP]; Gattas, Edwil Aparecida de Lucca [UNESP]

Year: 2014

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://dx.doi.org/10.1016/j.molcatb.2010.01.008

Thesis Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review

Silva, Viviane C. [UNESP]; Peres, Maristela F. S. [UNESP]; Gattas, Edwil Aparecida de Lucca [UNESP] · 2014 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review

Authors: Silva, Viviane C. [UNESP]; Peres, Maristela F. S. [UNESP]; Gattas, Edwil Aparecida de Lucca [UNESP]

Year: 2014

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://world-food.net/application-of-methylotrophic-yeast-pichia-pastoris-in-the-field-of-food-industry-a-review/

Thesis Optimal conditions for biomass and recombinant glycerol kinase production using the yeast Pichia pastoris

Aizemberg, Raquel [UNESP]; Terrazas, Werner D.M. [UNESP]; Ferreira-Dias, Suzana et al. · 2022 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Optimal conditions for biomass and recombinant glycerol kinase production using the yeast Pichia pastoris

Authors: Aizemberg, Raquel [UNESP]; Terrazas, Werner D.M. [UNESP]; Ferreira-Dias, Suzana; Valentini, Sandro R. [UNESP]; Gattás, Edwil A.L. [UNESP]

Year: 2022

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://hdl.handle.net/11449/219726

Thesis Heterologous expression of GH11 xylanase from Myceliophthora heterothallica F.2.1.4 in Pichia pastoris

de Amo, Gabriela Salvador; Bezerra-Bussoli, Carolina [UNESP]; Rodrigues da Silva, Ronivaldo [UNESP] et al. · 2025 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Heterologous expression of GH11 xylanase from Myceliophthora heterothallica F.2.1.4 in Pichia pastoris

Authors: de Amo, Gabriela Salvador; Bezerra-Bussoli, Carolina [UNESP]; Rodrigues da Silva, Ronivaldo [UNESP]; Kishi, Luciano Takeshi; Ferreira, Henrique [UNESP]; Gomes, Eleni [UNESP]; Bonilla-Rodriguez, Gustavo Orlando [UNESP]

Year: 2025

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://dx.doi.org/10.1016/j.bcab.2024.103343

Thesis Optimal Conditions for Biomass and Recombinant Glycerol Kinase Production Using the Yeast Pichia pastoris

Aizemberg, Raquel [UNESP]; Terrazas, Werner D. M. [UNESP]; Ferreira-Dias, Suzana et al. · 2014 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Optimal Conditions for Biomass and Recombinant Glycerol Kinase Production Using the Yeast Pichia pastoris

Authors: Aizemberg, Raquel [UNESP]; Terrazas, Werner D. M. [UNESP]; Ferreira-Dias, Suzana; Valentini, Sandro Roberto [UNESP]; Gattas, Edwil Aparecida de Lucca [UNESP]

Year: 2014

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://www.ftb.com.hr/49/FTB_49_329.html

Thesis High-Level Expression of a Functional Humanized Anti-CTLA4 Single-Chain Variable Fragment Antibody in Pichia Pastoris

Cai, Huawei; Chen, Lihong; Wan, Lin et al. · 2009 · Hong Kong University of Science and Technology (HKUST) · DOI

Full title: High-Level Expression of a Functional Humanized Anti-CTLA4 Single-Chain Variable Fragment Antibody in Pichia Pastoris

Authors: Cai, Huawei; Chen, Lihong; Wan, Lin; Zeng, Lingyu; Yang, Hao; Li, Shengfu; Li, Youping; Cheng, Jingqiu; Lu, Xiaofeng

Year: 2009

Venue: Hong Kong University of Science and Technology (HKUST)

DOI: http://repository.hkust.edu.hk/ir/Record/1783.1-17754

Thesis High-throughput process development from gene cloning to protein production

Sun, Manman; Gao, Alex Xiong; Liu, Xiuxia et al. · 2023 · Hong Kong University of Science and Technology (HKUST) · DOI

Full title: High-throughput process development from gene cloning to protein production

Authors: Sun, Manman; Gao, Alex Xiong; Liu, Xiuxia; Yang, Yankun; Ledesma-amaro, Rodrigo; Bai, Zhonghu

Year: 2023

Venue: Hong Kong University of Science and Technology (HKUST)

DOI: http://repository.hkust.edu.hk/ir/Record/1783.1-130355

Thesis Development of High Cell Density 30 L Fed-Batch Pichia pastoris Fermentation and Optimization of in situ Methanol Probe Response for Automated Control of Recombinant Protein Expression.

Sigmon, Jamie Danielle · 2014 · North Carolina State University · DOI

Full title: Development of High Cell Density 30 L Fed-Batch Pichia pastoris Fermentation and Optimization of in situ Methanol Probe Response for Automated Control of Recombinant Protein Expression.

Authors: Sigmon, Jamie Danielle

Year: 2014

Venue: North Carolina State University

DOI: http://www.lib.ncsu.edu/resolver/1840.16/9230

Thesis Factors Affecting the Specific Productivity of Pichia pastoris

Aw, Rochelle · 2013 · Imperial College London · DOI

Full title: Factors Affecting the Specific Productivity of Pichia pastoris

Authors: Aw, Rochelle

Year: 2013

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/11047

Thesis The expression of human leukemia inhibitory factor in Pichia pastoris

Zhang, Xiyuan · 2022 · University of Sydney · DOI

Full title: The expression of human leukemia inhibitory factor in Pichia pastoris

Authors: Zhang, Xiyuan

Year: 2022

Venue: University of Sydney

DOI: https://hdl.handle.net/2123/29919

Thesis Pichia pastoris as an expression host for membrane protein structural biology

Byrne, B · 2015 · Imperial College London · DOI

Full title: Pichia pastoris as an expression host for membrane protein structural biology

Authors: Byrne, B

Year: 2015

Venue: Imperial College London

DOI: http://www.sciencedirect.com/science/article/pii/S0959440X15000068

Thesis Modelling as a tool for increasing the specific productivity of single-chain antibody fragments from Pichia pastoris

Royle, Kate · 2015 · Imperial College London · DOI

Full title: Modelling as a tool for increasing the specific productivity of single-chain antibody fragments from Pichia pastoris

Authors: Royle, Kate

Year: 2015

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/24959

Thesis Structural Studies on Microneme proteins from Toxoplasma gondii

Liew, Lloyd · 2013 · Imperial College London · DOI

Full title: Structural Studies on Microneme proteins from Toxoplasma gondii

Authors: Liew, Lloyd

Year: 2013

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/11654

Thesis Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris

Edwards-Jones, B; Aw, R; Barton, GR et al. · 2015 · Imperial College London · DOI

Full title: Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris

Authors: Edwards-Jones, B; Aw, R; Barton, GR; Tredwell, GD; Bundy, JG; Leak, DJ

Year: 2015

Venue: Imperial College London

DOI: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000352138500124&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202

Thesis Can too many copies spoil the broth?

Aw, R; Polizzi, KM · 2016 · Imperial College London · DOI

Full title: Can too many copies spoil the broth?

Authors: Aw, R; Polizzi, KM

Year: 2016

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/29006

Thesis Cascaded de novo biosynthesis of lacto-proteins from CO2 by engineered Pichia pastoris

Lv, Xueqin; Cui, Shixiu; Chen, Jie et al. · 2024 · Imperial College London · DOI

Full title: Cascaded de novo biosynthesis of lacto-proteins from CO2 by engineered Pichia pastoris

Authors: Lv, Xueqin; Cui, Shixiu; Chen, Jie; Wang, Lingrui; Liu, Yanfeng; Li, Jianghua; Du, Guocheng; Liu, Xiaohao; Chen, Jian; Ledesma-Amaro, Rodrigo; Liu, Long

Year: 2024

Venue: Imperial College London

DOI: https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:001020551500001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb

Thesis Insights into the prevalence and underlying causes of clonal variation through transcriptomic analysis in Pichia pastoris

Aw, R; Barton, GR; Leak, DJ · 2018 · Imperial College London · DOI

Full title: Insights into the prevalence and underlying causes of clonal variation through transcriptomic analysis in Pichia pastoris

Authors: Aw, R; Barton, GR; Leak, DJ

Year: 2018

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/56710

Thesis Improving microbial chemical production strains through transcriptional regulatory network rewiring

Rodrigues, Rui Tiago de Lima · 2016 · Imperial College London · DOI

Full title: Improving microbial chemical production strains through transcriptional regulatory network rewiring

Authors: Rodrigues, Rui Tiago de Lima

Year: 2016

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/30834

Thesis Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris

Aw; McKay; Shattock et al. · 2017 · Imperial College London · DOI

Full title: Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris

Authors: Aw; McKay; Shattock; Polizzi, KM

Year: 2017

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/45699

Thesis A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris

Royle, KE; Polizzi, KM · 2017 · Imperial College London · DOI

Full title: A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris

Authors: Royle, KE; Polizzi, KM

Year: 2017

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/53329

Thesis Liquid PTVA: A faster and cheaper alternative for generating multi-copy clones in Pichia pastoris

Aw, R; Polizzi, KM · 2016 · Imperial College London · DOI

Full title: Liquid PTVA: A faster and cheaper alternative for generating multi-copy clones in Pichia pastoris

Authors: Aw, R; Polizzi, KM

Year: 2016

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/29028

Thesis Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling

Tredwell, GD; Leak, DJ; Aw, R et al. · 2017 · Imperial College London · DOI

Full title: Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling

Authors: Tredwell, GD; Leak, DJ; Aw, R; Edwards-Jones, B; Bundy, JG

Year: 2017

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/43713

Thesis A systematic analysis of the expression of the anti-HIV VRC01 antibody in Pichia pastoris through signal peptide optimization

Aw, Rochelle; McKay, Paul; Shattock, Robin et al. · 2018 · Imperial College London · DOI

Full title: A systematic analysis of the expression of the anti-HIV VRC01 antibody in Pichia pastoris through signal peptide optimization

Authors: Aw, Rochelle; McKay, Paul; Shattock, Robin; Polizzi, Karen

Year: 2018

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/58612

Thesis Methods for expression of recombinant proteins using a Pichia pastoris cell-free system

Aw, Rochelle; Spice, Alex J; Polizzi, Karen · 2020 · Imperial College London · DOI

Full title: Methods for expression of recombinant proteins using a Pichia pastoris cell-free system

Authors: Aw, Rochelle; Spice, Alex J; Polizzi, Karen

Year: 2020

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/83872

Thesis Knockout of RSN1, TVP18 or CSC1‐2 causes perturbation of Golgi cisternae in Pichia pastoris

Aw, Rochelle; De Wachter, Charlot; Laukens, Bram et al. · 2020 · Imperial College London · DOI

Full title: Knockout of RSN1, TVP18 or CSC1‐2 causes perturbation of Golgi cisternae in Pichia pastoris

Authors: Aw, Rochelle; De Wachter, Charlot; Laukens, Bram; De Rycke, Riet; De Bruyne, Michiel; Bell, David; Callewaert, Nico; Polizzi, Karen M

Year: 2020

Venue: Imperial College London

DOI: https://onlinelibrary.wiley.com/doi/10.1111/tra.12773

Thesis Engineering microbialm phenotypes through rewiring of genetic networks

Windram, OPF; Rodrigues, RTL; Lee, S et al. · 2017 · Imperial College London · DOI

Full title: Engineering microbialm phenotypes through rewiring of genetic networks

Authors: Windram, OPF; Rodrigues, RTL; Lee, S; Bayer, TS

Year: 2017

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/45620

Thesis Production and purification of an active CRM197 in Pichia pastoris and its immunological characterization using a Vi-typhoid antigen vaccine

Aw, Rochelle; Ashik, Muktadir R; Islam, Ariful AZM et al. · 2022 · Imperial College London · DOI

Full title: Production and purification of an active CRM197 in Pichia pastoris and its immunological characterization using a Vi-typhoid antigen vaccine

Authors: Aw, Rochelle; Ashik, Muktadir R; Islam, Ariful AZM; Khan, Imran; Mainuddin, Mohammad; Islam, Md Ashraful; Ahasan, Mohammad M; Polizzi, Karen M

Year: 2022

Venue: Imperial College London

DOI: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000731300100006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202

Thesis Towards the cell-free expression of glycoproteins

Exley, Kealan Peter · 2019 · Imperial College London · DOI

Full title: Towards the cell-free expression of glycoproteins

Authors: Exley, Kealan Peter

Year: 2019

Venue: Imperial College London

DOI: http://hdl.handle.net/10044/1/68742

Thesis Biosensor‐assisted engineering of a high‐yield Pichia pastoris cell‐free protein synthesis platform

Aw, Rochelle; Polizzi, Karen M · 2018 · Imperial College London · DOI

Full title: Biosensor‐assisted engineering of a high‐yield Pichia pastoris cell‐free protein synthesis platform

Authors: Aw, Rochelle; Polizzi, Karen M

Year: 2018

Venue: Imperial College London

DOI: https://onlinelibrary.wiley.com/doi/full/10.1002/bit.26901

Thesis Murine factor H Co-produced in yeast with protein disulfide isomerase ameliorated C3 dysregulation in factor H-deficient mice

Kerr, Heather; Herbert, Andrew P; Makou, Elisavet et al. · 2021 · Imperial College London · DOI

Full title: Murine factor H Co-produced in yeast with protein disulfide isomerase ameliorated C3 dysregulation in factor H-deficient mice

Authors: Kerr, Heather; Herbert, Andrew P; Makou, Elisavet; Abramczyk, Dariusz; Malik, Talat H; Lomax-Browne, Hannah; Yang, Yi; Pappworth, Isabel Y; Denton, Harriet; Richards, Anna; Marchbank, Kevin J; Pickering, Matthew C; Barlow, Paul N

Year: 2021

Venue: Imperial College London

DOI: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000654216800001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202

Thesis Effects of different media supplements on the production of an active recombinant plant peroxidase in a Pichia pastoris Δoch1 strain

Gmeiner, Christoph; Spadiut, Oliver · 2015 · ETH Zurich (Swiss Federal Institute of Technology in Zurich) · DOI

Full title: Effects of different media supplements on the production of an active recombinant plant peroxidase in a Pichia pastoris Δoch1 strain

Authors: Gmeiner, Christoph; Spadiut, Oliver

Year: 2015

Venue: ETH Zurich (Swiss Federal Institute of Technology in Zurich)

DOI: http://hdl.handle.net/20.500.11850/107069

Thesis High‐Throughput Optimization of Recombinant Protein Production in Microfluidic Gel Beads

Napiorkowska, Marta; Pestalozzi, Luzius; Panke, Sven et al. · 2021 · ETH Zurich (Swiss Federal Institute of Technology in Zurich) · DOI

Full title: High‐Throughput Optimization of Recombinant Protein Production in Microfluidic Gel Beads

Authors: Napiorkowska, Marta; Pestalozzi, Luzius; Panke, Sven; id_orcid0000-0002-9368-539X; Held, Martin; Schmitt, Steven

Year: 2021

Venue: ETH Zurich (Swiss Federal Institute of Technology in Zurich)

DOI: http://hdl.handle.net/20.500.11850/458699

Thesis From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pastoris

Tir, Nora; Heistinger, Lina; Grünwald-Gruber, Clemens et al. · 2022 · ETH Zurich (Swiss Federal Institute of Technology in Zurich) · DOI

Full title: From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pastoris

Authors: Tir, Nora; Heistinger, Lina; Grünwald-Gruber, Clemens; Jakob, Leo A.; Dickgiesser, Stephan; Rasche, Nicolas; Mattanovich, Diethard

Year: 2022

Venue: ETH Zurich (Swiss Federal Institute of Technology in Zurich)

DOI: http://hdl.handle.net/20.500.11850/565141

Thesis Engineered bidirectional promoters enable rapid multi-gene co-expression optimization

Vogl, Thomas; Kickenweiz, Thomas; Pitzer, Julia et al. · 2018 · ETH Zurich (Swiss Federal Institute of Technology in Zurich) · DOI

Full title: Engineered bidirectional promoters enable rapid multi-gene co-expression optimization

Authors: Vogl, Thomas; Kickenweiz, Thomas; Pitzer, Julia; Sturmberger, Lukas; Weninger, Astrid; Biggs, Bradley W.; Köhler, Eva-Maria; Baumschlager, Armin; Fischer, Jasmin E.; Hyden, Patrick; Wagner, Marlies; Baumann, Martina; Borth, Nicole; Geier, Martina; Ajikumar, Parayil K.; Glieder, Anton

Year: 2018

Venue: ETH Zurich (Swiss Federal Institute of Technology in Zurich)

DOI: http://hdl.handle.net/20.500.11850/287964

Thesis Utilización de glicerol proveniente de la industria de biodiesel como fuente de carbono para la producción de lipasas recombinantes en Pichia pastoris.

Loango Chamorro, Nelsy · 2015 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Utilización de glicerol proveniente de la industria de biodiesel como fuente de carbono para la producción de lipasas recombinantes en Pichia pastoris.

Authors: Loango Chamorro, Nelsy

Year: 2015

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/313455

Thesis The pCri System: A Vector Collection for Recombinant Protein Expression and Purification

Goulas, Theodoros; Cuppari, Anna; Garcia-Castellanos, Raquel et al. · 2014 · ETH Zurich (Swiss Federal Institute of Technology in Zurich) · DOI

Full title: The pCri System: A Vector Collection for Recombinant Protein Expression and Purification

Authors: Goulas, Theodoros; Cuppari, Anna; Garcia-Castellanos, Raquel; Snipas, Scott; Glockshuber, Rudi; Arolas, Joan L.; Gomis-Rueth, F. Xavier

Year: 2014

Venue: ETH Zurich (Swiss Federal Institute of Technology in Zurich)

DOI: http://hdl.handle.net/20.500.11850/93676

Thesis Estudio de la producción heteróloga de una lipasa del hongo Rhizopus oryzae en la levadura metilotrófica Pichia pastoris

Serrano Gonzàlez, Alícia · 2011 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Estudio de la producción heteróloga de una lipasa del hongo Rhizopus oryzae en la levadura metilotrófica Pichia pastoris

Authors: Serrano Gonzàlez, Alícia

Year: 2011

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://www.tdx.cat/TDX-0701104-165829

Thesis Producción de la lipasa LIP2 de Candida rugosa en el sistema Pichia pastoris: caracterización y aplicación en reacciones de síntesis

Alarcón Vivero, Manuel Rubén · 2011 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Producción de la lipasa LIP2 de Candida rugosa en el sistema Pichia pastoris: caracterización y aplicación en reacciones de síntesis

Authors: Alarcón Vivero, Manuel Rubén

Year: 2011

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://www.tdx.cat/TDX-0323109-155527

Thesis Estratègies d'operació en el procés de producció de proteïnes heteròlogues en Pichia pastoris: aplicació de tècniques de monitorització i control

Ramon Real, Ramon · 2011 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Estratègies d'operació en el procés de producció de proteïnes heteròlogues en Pichia pastoris: aplicació de tècniques de monitorització i control

Authors: Ramon Real, Ramon

Year: 2011

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://www.tdx.cat/TDX-0121110-092632

Thesis Expression of the Rhizopus oryzae lipase in Pichia pastoris under the control of the FLD1 promoter

Resina Rodríguez, David · 2011 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Expression of the Rhizopus oryzae lipase in Pichia pastoris under the control of the FLD1 promoter

Authors: Resina Rodríguez, David

Year: 2011

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://www.tdx.cat/TDX-0412107-155334

Thesis Monitorització i control del procés de producció de proteïnes heteròlogues en el llevat metilotròfic Pichia Pastoris

Cos Busquets, Oriol · 2011 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Monitorització i control del procés de producció de proteïnes heteròlogues en el llevat metilotròfic Pichia Pastoris

Authors: Cos Busquets, Oriol

Year: 2011

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://www.tdx.cat/TDX-0313106-175720

Thesis Producció recombinant de la gonadotrofina coriònica equina amb el llevat Pichia pastoris

Ubach Font, Anna · 2011 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Producció recombinant de la gonadotrofina coriònica equina amb el llevat Pichia pastoris

Authors: Ubach Font, Anna

Year: 2011

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://www.tdx.cat/TDX-0414110-153424

Thesis Providing growth-decoupled strategies for recombinant protein production in Komagataella phaffii: from promoter identification to bioprocess scale-up

Bernat Camps, Núria · 2024 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Providing growth-decoupled strategies for recombinant protein production in Komagataella phaffii: from promoter identification to bioprocess scale-up

Authors: Bernat Camps, Núria

Year: 2024

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/693041

Thesis Millora en el procés de producció d'una lipasa de Rhizopus oryzae en Pichia pastoris mitjançant tècniques de monitoratge i estratègies de cultiu alternatives

Surribas i Casalprim, Anna · 2011 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Millora en el procés de producció d'una lipasa de Rhizopus oryzae en Pichia pastoris mitjançant tècniques de monitoratge i estratègies de cultiu alternatives

Authors: Surribas i Casalprim, Anna

Year: 2011

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://www.tdx.cat/TDX-0217110-131258

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2. Data Extraction

0/1039 extracted

Paper

Secreted production of an elastin-like polypeptide by Pichia pastoris

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DOI: https://doi.org/10.1007/s00253-014-6346-7

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Full title: Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfolded proteins using chemostat cultures

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Full title: High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances and bioactive precursors

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DOI: https://doi.org/10.1007/s00253-020-10878-4

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DOI: https://doi.org/10.1007/s00253-021-11337-4

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DOI: https://doi.org/10.1007/s00449-012-0848-4

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Full title: Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins.

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DOI: https://doi.org/10.1007/s00253-021-11336-5

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DOI: https://doi.org/10.1007/s00253-023-12878-6

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DOI: https://doi.org/10.1186/1472-6750-11-23

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Full title: Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris

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DOI: https://doi.org/10.1186/1472-6750-11-47

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DOI: https://doi.org/10.1186/1471-2180-9-151

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DOI: https://doi.org/10.1186/1472-6750-7-14

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DOI: https://doi.org/10.1186/1471-2164-12-218

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DOI: https://doi.org/10.1186/1472-6750-13-38

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DOI: https://doi.org/10.1186/1472-6750-12-75

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DOI: https://doi.org/10.1186/1472-6750-13-26

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M. Muraki · 2014

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DOI: https://doi.org/10.1186/1472-6750-14-19

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DOI: https://doi.org/10.1186/1472-6750-13-71

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Full title: Efficient production of human bivalent and trivalent anti-MUC1 Fab-scFv antibodies in Pichia pastoris

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DOI: https://doi.org/10.1186/1472-6750-9-70

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DOI: https://doi.org/10.1186/1475-2859-10-14

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Full title: A thermostable GH45 endoglucanase from yeast: impact of its atypical multimodularity on activity

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DOI: https://doi.org/10.1186/1475-2859-10-103

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Full title: Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes

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DOI: https://doi.org/10.1186/1475-2859-10-113

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DOI: https://doi.org/10.1186/1472-6750-12-51

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DOI: https://doi.org/10.1186/1475-2859-10-106

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Full title: The response to unfolded protein is involved in osmotolerance of Pichia pastoris

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DOI: https://doi.org/10.1186/1471-2164-11-207

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Full title: Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris

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DOI: https://doi.org/10.1186/1475-2859-11-102

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Dietzsch, Christian; Spadiut, Oliver; Herwig, Christoph · 2011

Full title: A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains

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DOI: https://doi.org/10.1186/1475-2859-10-85

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Full title: Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments

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DOI: https://doi.org/10.1186/1475-2859-10-34

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Full title: Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen

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DOI: https://doi.org/10.1186/1475-2859-10-48

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Pfeffer, Martin; Maurer, Michael; Köllensperger, Gunda et al. · 2011

Full title: Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedure

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DOI: https://doi.org/10.1186/1475-2859-10-47

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Kristin Baumann; Núria Adelantado; C. Lang et al. · 2011

Full title: Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris

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DOI: https://doi.org/10.1186/1475-2859-10-93

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Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield

Routledge, Sarah J; Hewitt, Christopher J.; Bora, Nagamani et al. · 2011

Full title: Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield

Authors: Routledge, Sarah J; Hewitt, Christopher J.; Bora, Nagamani; Bill, Roslyn M.

DOI: https://doi.org/10.1186/1475-2859-10-17

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DOI: https://doi.org/10.1186/1475-2859-11-103

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Full title: Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

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DOI: https://doi.org/10.1186/1475-2859-11-57

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A. Mellitzer; R. Weis; A. Glieder et al. · 2012

Full title: Expression of lignocellulolytic enzymes in Pichia pastoris

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DOI: https://doi.org/10.1186/1475-2859-11-61

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Full title: Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33

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DOI: https://doi.org/10.1186/1475-2859-11-25

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Systems metabolic engineering, industrial biotechnology and microbial cell factories

Lee, Sang Yup; Mattanovich, Diethard; Villaverde, Antonio · 2012

Full title: Systems metabolic engineering, industrial biotechnology and microbial cell factories

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DOI: https://doi.org/10.1186/1475-2859-11-156

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Marc Carnicer; A. ten Pierick; J. V. van Dam et al. · 2012

Full title: Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions

Authors: Marc Carnicer; A. ten Pierick; J. V. van Dam; J. Heijnen; J. Albiol; W. V. van Gulik; P. Ferrer

DOI: https://doi.org/10.1186/1475-2859-11-83

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Full title: Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

DOI: https://doi.org/10.1186/1475-2859-11-78

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Guerfal, Mouna; Claes, Katrien; Knittelfelder, Oskar et al. · 2013

Full title: Enhanced membrane protein expression by engineering increased intracellular membrane production

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DOI: https://doi.org/10.1186/1475-2859-12-122

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Chen, Ming‐Tang; Lin, Song; Shandil, Ishaan et al. · 2012

Full title: Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

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DOI: https://doi.org/10.1186/1475-2859-11-91

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Full title: Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES.

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DOI: https://doi.org/10.1186/1475-2859-11-47

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Dashtban, Mehdi; Qin, Wensheng · 2012

Full title: Overexpression of an exotic thermotolerant β-glucosidase in trichoderma reesei and its significant increase in cellulolytic activity and saccharification of barley straw

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DOI: https://doi.org/10.1186/1475-2859-11-63

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Full title: Recombinant sterol esterase from Ophiostoma piceae: an improved biocatalyst expressed in Pichia pastoris

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DOI: https://doi.org/10.1186/1475-2859-11-73

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Full title: Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

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DOI: https://doi.org/10.1186/1475-2859-12-5

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Authors: Ana Letícia Vanz; M. Nimtz; U. Rinas

DOI: https://doi.org/10.1186/1475-2859-13-23

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Fazenda, Mariana L.; Dias, João M.L.; Harvey, Linda M. et al. · 2013

Full title: Towards better understanding of an industrial cell factory: investigating the feasibility of real-time metabolic flux analysis in Pichia pastoris

Authors: Fazenda, Mariana L.; Dias, João M.L.; Harvey, Linda M.; Nordon, Alison; Edrada‐Ebel, RuAngelie; Littlejohn, David; McNeil, Brian

DOI: https://doi.org/10.1186/1475-2859-12-51

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Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

Gao, Limei; Cai, Menghao; Shen, Wei et al. · 2013

Full title: Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

Authors: Gao, Limei; Cai, Menghao; Shen, Wei; Xiao, Siwei; Zhou, Xiangshan; Zhang, Yuanxing

DOI: https://doi.org/10.1186/1475-2859-12-77

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Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

J. Hemmerich; Núria Adelantado; J. M. Barrigón et al. · 2014

Authors: J. Hemmerich; Núria Adelantado; J. M. Barrigón; Xavier Ponte; Astrid Hörmann; P. Ferrer; F. Kensy; F. Valero

DOI: https://doi.org/10.1186/1475-2859-13-36

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Niu, Hongxing; Jost, Laurent; Pirlot, Nathalie et al. · 2013

Full title: A quantitative study of methanol/sorbitol co-feeding process of a Pichia pastoris Mut+/pAOX1-lacZ strain

Authors: Niu, Hongxing; Jost, Laurent; Pirlot, Nathalie; Sassi, Hosni; Daukandt, Marc; Rodriguez, Christian; Fickers, Patrick

DOI: https://doi.org/10.1186/1475-2859-12-33

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Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production

Sygmund, Christoph; Santner, Paul; Krondorfer, Iris et al. · 2013

Full title: Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production

Authors: Sygmund, Christoph; Santner, Paul; Krondorfer, Iris; Peterbauer, Clemens; Alcalde, Miguel; Nyanhongo, Gibson S.; Guebitz, Georg M.; Ludwig, Roland

DOI: https://doi.org/10.1186/1475-2859-12-38

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Yeast biotechnology: teaching the old dog new tricks

Mattanovich, Diethard; Sauer, Michael; Gasser, Brigitte · 2014

Full title: Yeast biotechnology: teaching the old dog new tricks

Authors: Mattanovich, Diethard; Sauer, Michael; Gasser, Brigitte

DOI: https://doi.org/10.1186/1475-2859-13-34

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Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum

Matsuda, Yoshihiko; Itaya, Hiroshi; Kitahara, Yuki et al. · 2014

Full title: Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum

Authors: Matsuda, Yoshihiko; Itaya, Hiroshi; Kitahara, Yuki; Theresia, Natalia Maria; Kutukova, Ekaterina A.; Yomantas, Yurgis Antanas Vladovich; Date, Masayo; Kikuchi, Yoshimi; Wachi, Masaaki

DOI: https://doi.org/10.1186/1475-2859-13-56

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Expression of endoglucanases in Pichia pastoris under control of the GAP promoter

Várnai, Anikó; Tang, Campbell; Bengtsson, Oskar et al. · 2014

Full title: Expression of endoglucanases in Pichia pastoris under control of the GAP promoter

Authors: Várnai, Anikó; Tang, Campbell; Bengtsson, Oskar; Atterton, Andrew; Mathiesen, Geir; Eijsink, Vincent G. H.

DOI: https://doi.org/10.1186/1475-2859-13-57

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Carbon source dependent promoters in yeasts

Weinhandl, Katrin; Winkler, Margit; Glieder, Anton et al. · 2014

Full title: Carbon source dependent promoters in yeasts

Authors: Weinhandl, Katrin; Winkler, Margit; Glieder, Anton; Camattari, Andrea

DOI: https://doi.org/10.1186/1475-2859-13-5

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Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

Sauer, Michael; Branduardi, Paola; Gasser, Brigitte et al. · 2004

Full title: Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

Authors: Sauer, Michael; Branduardi, Paola; Gasser, Brigitte; Valli, Minoska; Maurer, Michael; Porro, Danilo; Mattanovich, Diethard

DOI: https://doi.org/10.1186/1475-2859-3-17

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Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris

Gürkan, Cemal; Ellar, David J. · 2005

Full title: Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris

Authors: Gürkan, Cemal; Ellar, David J.

DOI: https://doi.org/10.1186/1475-2859-4-33

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Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review

Cós, Oriol; Ramón, Ramón; Montesinos, José Luis González et al. · 2006

Full title: Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review

Authors: Cós, Oriol; Ramón, Ramón; Montesinos, José Luis González; Valero, Francisco

DOI: https://doi.org/10.1186/1475-2859-5-17

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The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi

Joosten, Vivi; Lokman, Christien; Hondel, Cees A. M. J. J. van den et al. · 2003

Full title: The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi

Authors: Joosten, Vivi; Lokman, Christien; Hondel, Cees A. M. J. J. van den; Punt, Peter J.

DOI: https://doi.org/10.1186/1475-2859-2-1

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Regulation of methanol utilisation pathway genes in yeasts.

Hartner, Franz Stefan; Glieder, Anton · 2006

Full title: Regulation of methanol utilisation pathway genes in yeasts.

Authors: Hartner, Franz Stefan; Glieder, Anton

DOI: https://doi.org/10.1186/1475-2859-5-39

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Application of a wide-range yeast vector (CoMed™) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts

Steinborn, Gerhard; Böer, Erik; Scholz, Anja et al. · 2006

Full title: Application of a wide-range yeast vector (CoMed™) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts

Authors: Steinborn, Gerhard; Böer, Erik; Scholz, Anja; Tag, Kristina; Kunze, Gotthard; Gellissen, Gerd

DOI: https://doi.org/10.1186/1475-2859-5-33

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Heterologous expression of lcc1 gene from Trametes trogii in Pichia pastoris and characterization of the recombinant enzyme.

Colao, M. Ch.; Lupino, Stefania; Garzillo, Anna Maria Vittoria et al. · 2006

Full title: Heterologous expression of lcc1 gene from Trametes trogii in Pichia pastoris and characterization of the recombinant enzyme.

Authors: Colao, M. Ch.; Lupino, Stefania; Garzillo, Anna Maria Vittoria; Buonocore, Vincenzo; Ruzzi, Maurizio

DOI: https://doi.org/10.1186/1475-2859-5-31

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Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris

Maurer, Michael; Kühleitner, Manfred; Gasser, Brigitte et al. · 2006

Full title: Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris

Authors: Maurer, Michael; Kühleitner, Manfred; Gasser, Brigitte; Mattanovich, Diethard

DOI: https://doi.org/10.1186/1475-2859-5-37

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Role of cultivation media in the development of yeast strains for large scale industrial use

Hahn‐Hägerdal, Bärbel; Karhumaa, Kaisa; Larsson, Christer et al. · 2005

Full title: Role of cultivation media in the development of yeast strains for large scale industrial use

Authors: Hahn‐Hägerdal, Bärbel; Karhumaa, Kaisa; Larsson, Christer; Gorwa‐Grauslund, Marie F.; Görgens, Johann F.; Zyl, Willem H. van

DOI: https://doi.org/10.1186/1475-2859-4-31

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Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris

Surribas, Anna; Resina, David; Ferrer, Pau et al. · 2007

Full title: Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris

Authors: Surribas, Anna; Resina, David; Ferrer, Pau; Valero, Francisco

DOI: https://doi.org/10.1186/1475-2859-6-15

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Transcriptional analysis of protein production and induction of unfolded protein response in Pichia pastoris expressing a Rhizopus oryzae lipase under the FLD1 promoter

David Resina; M. Bollók; F. Valero et al. · 2006

Full title: Transcriptional analysis of protein production and induction of unfolded protein response in Pichia pastoris expressing a Rhizopus oryzae lipase under the FLD1 promoter

Authors: David Resina; M. Bollók; F. Valero; P. Neubauer; P. Ferrer

DOI: https://doi.org/10.1186/1475-2859-5-s1-p49

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Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

Gasser, Brigitte; Saloheimo, Markku; Rinas, Ursula et al. · 2008

Full title: Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

DOI: https://doi.org/10.1186/1475-2859-7-11

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Engineering and Applications of fungal laccases for organic synthesis

Kunamneni, Adinarayana; Camarero, Susana; García-Burgos, Carlos et al. · 2008

Full title: Engineering and Applications of fungal laccases for organic synthesis

Authors: Kunamneni, Adinarayana; Camarero, Susana; García-Burgos, Carlos; Plou, Francisco J.; Ballesteros, Antonio; Alcalde, Miguel

DOI: https://doi.org/10.1186/1475-2859-7-32

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Substrate feeding strategies in Pichia pastoris fed-batch cultivation processes: Analysis of key parameters influencing recombinant protein production

Ramón, Ramón; Cós, Oriol; Ferrer, Pau et al. · 2006

Full title: Substrate feeding strategies in Pichia pastoris fed-batch cultivation processes: Analysis of key parameters influencing recombinant protein production

Authors: Ramón, Ramón; Cós, Oriol; Ferrer, Pau; Montesinos, José Luis González; Valero, Francisco

DOI: https://doi.org/10.1186/1475-2859-5-s1-s13

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Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

Mattanovich, Diethard; Graf, Alexandra B.; Stadlmann, Johannes et al. · 2009

Full title: Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

Authors: Mattanovich, Diethard; Graf, Alexandra B.; Stadlmann, Johannes; Dragosits, Martin; Redl, Andréas; Maurer, Michael; Kleinheinz, Martin; Sauer, Michael; Altmann, Friedrich; Gasser, Brigitte

DOI: https://doi.org/10.1186/1475-2859-8-29

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Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

Hans Marx; D. Mattanovich; M. Sauer · 2008

Full title: Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

Authors: Hans Marx; D. Mattanovich; M. Sauer

DOI: https://doi.org/10.1186/1475-2859-7-23

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Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase

Rodríguez, Ángel Pereira; Fernández-Leiro, Rafael; Trillo, M Cristina et al. · 2006

Full title: Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase

Authors: Rodríguez, Ángel Pereira; Fernández-Leiro, Rafael; Trillo, M Cristina; Cerdán, M. Esperanza; González-Siso, María-Isabel; Becerra, Manuel

DOI: https://doi.org/10.1186/1475-2859-5-41

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Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels

Carnicer, Marc; Baumann, Kristin; Töplitz, Isabelle et al. · 2009

Full title: Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels

Authors: Carnicer, Marc; Baumann, Kristin; Töplitz, Isabelle; Sánchez-Ferrando, Francesc; Mattanovich, Diethard; Ferrer, Pau; Albiol, Joan

DOI: https://doi.org/10.1186/1475-2859-8-65

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Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

Bonander, Nicklas; Darby, Richard A. J.; Grgic, Ljuban et al. · 2009

Full title: Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

Authors: Bonander, Nicklas; Darby, Richard A. J.; Grgic, Ljuban; Bora, Nagamani; Wen, Jikai; Brogna, Saverio; Poyner, David R.; O’Neill, Michael; Bill, Roslyn M.

DOI: https://doi.org/10.1186/1475-2859-8-10

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Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction.

Resina, David; Bollók, Mónika; Khatri, Narendar K et al. · 2007

Full title: Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction.

Authors: Resina, David; Bollók, Mónika; Khatri, Narendar K; Valero, Francisco; Neubauer, Peter; Ferrer, Pau

DOI: https://doi.org/10.1186/1475-2859-6-21

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Microbial factories for recombinant pharmaceuticals

Ferrer‐Miralles, Neus; Domingo‐Espín, Joan; Corchero, José Luís et al. · 2009

Full title: Microbial factories for recombinant pharmaceuticals

Authors: Ferrer‐Miralles, Neus; Domingo‐Espín, Joan; Corchero, José Luís; Vázquez, Esther; Villaverde, Antonio

DOI: https://doi.org/10.1186/1475-2859-8-17

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Yeast cell factory: fishing for the best one or engineering it?

Porro, Danilo; Branduardi, Paola · 2009

Full title: Yeast cell factory: fishing for the best one or engineering it?

Authors: Porro, Danilo; Branduardi, Paola

DOI: https://doi.org/10.1186/1475-2859-8-51

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Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

Krainer, Florian; Dietzsch, Christian; Hajek, Tanja et al. · 2012

Full title: Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

Authors: Krainer, Florian; Dietzsch, Christian; Hajek, Tanja; Herwig, Christoph; Spadiut, Oliver; Glieder, Anton

DOI: https://doi.org/10.1186/1475-2859-11-22

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Open access to sequence: Browsing the Pichia pastoris genome

Mattanovich, Diethard; Callewaert, Nico; Rouzé, Pierre et al. · 2009

Full title: Open access to sequence: Browsing the Pichia pastoris genome

Authors: Mattanovich, Diethard; Callewaert, Nico; Rouzé, Pierre; Lin, Yao‐Cheng; Graf, Alexandra B.; Redl, Andréas; Tiels, Petra; Gasser, Brigitte; Schutter, Kristof De

DOI: https://doi.org/10.1186/1475-2859-8-53

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Tuning microbial hosts for membrane protein production

Freigaßner, Maria Elisabeth; Pichler, Harald; Glieder, Anton · 2009

Full title: Tuning microbial hosts for membrane protein production

Authors: Freigaßner, Maria Elisabeth; Pichler, Harald; Glieder, Anton

DOI: https://doi.org/10.1186/1475-2859-8-69

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The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

Guerfal, Mouna; Ryckaert, Stefan; Jacobs, Pieter P. et al. · 2010

Full title: The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

Authors: Guerfal, Mouna; Ryckaert, Stefan; Jacobs, Pieter P.; Ameloot, Paul; Craenenbroeck, Kathleen Van; Derycke, Riet; Callewaert, Nico

DOI: https://doi.org/10.1186/1475-2859-9-49

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Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst

Abad, Sandra; Nahálka, Jozef; Bergler, Gabriele et al. · 2010

Full title: Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst

Authors: Abad, Sandra; Nahálka, Jozef; Bergler, Gabriele; Arnold, S. Alison; Speight, Robert; Fotheringham, Ian; Nidetzky, Bernd; Glieder, Anton

DOI: https://doi.org/10.1186/1475-2859-9-24

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Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands

K. Ettayebi; M. Hardy · 2008

Full title: Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands

Authors: K. Ettayebi; M. Hardy

DOI: https://doi.org/10.1186/1743-422x-5-21

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Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence

Bai, Yingguo; Wang, Jian‐She; Zhang, Zhifang et al. · 2010

Full title: Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence

Authors: Bai, Yingguo; Wang, Jian‐She; Zhang, Zhifang; Shi, Pengjun; Luo, Huiying; Huang, Huoqing; Luo, Chunliang; Yao, Bin

DOI: https://doi.org/10.1186/1475-2859-9-33

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Chen, Ruidong; Zhou, Zhigang; Cao, Yanan et al. · 2010

Full title: High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture

Authors: Chen, Ruidong; Zhou, Zhigang; Cao, Yanan; Bai, Yingguo; Yao, Bin

DOI: https://doi.org/10.1186/1475-2859-9-39

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Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement

Bevan Ks Chung; Suresh Selvarasu; Camattari Andrea et al. · 2010

Full title: Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement

Authors: Bevan Ks Chung; Suresh Selvarasu; Camattari Andrea; Jimyoung Ryu; H. Lee; Jungoh Ahn; Hongweon Lee; Dong-Yup Lee

DOI: https://doi.org/10.1186/1475-2859-9-50

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High yield expression of leptospirosis vaccine candidates LigA and LipL32 in the methylotrophic yeast Pichia pastoris

Hartwig, Daiane Drawanz; Oliveira, Thaís Larré; Seixas, Fabiana K. et al. · 2010

Full title: High yield expression of leptospirosis vaccine candidates LigA and LipL32 in the methylotrophic yeast Pichia pastoris

Authors: Hartwig, Daiane Drawanz; Oliveira, Thaís Larré; Seixas, Fabiana K.; Forster, Karine Maciel; Rizzi, Caroline; Hartleben, Cláudia Pinho; McBride, Alan J. A.; Dellagostin, Odir Antônio

DOI: https://doi.org/10.1186/1475-2859-9-98

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Pourcq, Karen De; Tiels, Petra; Hecke, Annelies Van et al. · 2012

Full title: Engineering Yarrowia lipolytica to Produce Glycoproteins Homogeneously Modified with the Universal Man3GlcNAc2 N-Glycan Core

Authors: Pourcq, Karen De; Tiels, Petra; Hecke, Annelies Van; Geysens, Steven; Vervecken, Wouter; Callewaert, Nico

DOI: https://doi.org/10.1371/journal.pone.0039976

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Full title: Implications of biodiesel production for enhancing thermostable T1.2RQ lipase secretion in Pichia pastoris via chaperone co-expression

Authors: E. Puspitasari; Y. Rustam; Andreas Adhi Satya; Antonius Suwanto; A. Wahyudi; R. Astuti

DOI: https://doi.org/10.13057/biodiv/d260927

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Codon Optimization Significantly Improves the Expression Level of a Keratinase Gene in Pichia pastoris

Hu, Hong; Gao, Jie; He, Jun et al. · 2013

Full title: Codon Optimization Significantly Improves the Expression Level of a Keratinase Gene in Pichia pastoris

Authors: Hu, Hong; Gao, Jie; He, Jun; Yu, Bing; Zheng, Ping; Huang, Zhiqing; Mao, Xiangbing; Yu, Jie; Han, Guoquan; Chen, Daiwen

DOI: https://doi.org/10.1371/journal.pone.0058393

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Anangi, Raveendra; Koshy, Shyny; Huq, Redwan et al. · 2012

Full title: Recombinant Expression of Margatoxin and Agitoxin-2 in Pichia pastoris: An Efficient Method for Production of KV1.3 Channel Blockers

Authors: Anangi, Raveendra; Koshy, Shyny; Huq, Redwan; Beeton, Christine; Chuang, Woei‐Jer; King, Glenn F.

DOI: https://doi.org/10.1371/journal.pone.0052965

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Shaheen, Hussam; Prinz, Bianka; Chen, Ming‐Tang et al. · 2013

Full title: A Dual-Mode Surface Display System for the Maturation and Production of Monoclonal Antibodies in Glyco-Engineered Pichia pastoris

Authors: Shaheen, Hussam; Prinz, Bianka; Chen, Ming‐Tang; Pavoor, Tej Venkatachalam; Lin, Song; Houston-Cummings, Nga Rewa; Moore, Renée; Stadheim, Terrance A.; Zha, Dongxing

DOI: https://doi.org/10.1371/journal.pone.0070190

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Chai, Ran; Qiu, Cuiwei; DongRen, Liu et al. · 2013

Full title: β-Glucan Synthase Gene Overexpression and β-Glucans Overproduction in Pleurotus ostreatus Using Promoter Swapping

Authors: Chai, Ran; Qiu, Cuiwei; DongRen, Liu; Qi, Yuancheng; Gao, Yuqian; Shen, Jinwen; Qiu, Liyou

DOI: https://doi.org/10.1371/journal.pone.0061693

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de novo Design and Synthesis of Candida antarctica Lipase B Gene and α-Factor Leads to High-Level Expression in Pichia pastoris

Yang, Jiangke; Liu, Liying; Dai, Jianghong et al. · 2013

Full title: de novo Design and Synthesis of Candida antarctica Lipase B Gene and α-Factor Leads to High-Level Expression in Pichia pastoris

Authors: Yang, Jiangke; Liu, Liying; Dai, Jianghong; Li, Qin

DOI: https://doi.org/10.1371/journal.pone.0053939

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Full title: Conformational and Thermal Stability Improvements for the Large-Scale Production of Yeast-Derived Rabbit Hemorrhagic Disease Virus-Like Particles as Multipurpose Vaccine

DOI: https://doi.org/10.1371/journal.pone.0056417

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Nett, Juergen H.; Cook, W. James; Mingtang, Chen et al. · 2013

Full title: Characterization of the Pichia pastoris Protein-O-mannosyltransferase Gene Family

DOI: https://doi.org/10.1371/journal.pone.0068325

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Song, Yang; Kuang, Ye; Li, Hongbo et al. · 2013

Full title: Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site

Authors: Song, Yang; Kuang, Ye; Li, Hongbo; Liu, Yue-Hong; Hui, Xiaoyan; Li, Peng; Jiang, Zhiwu; Zhou, Yulai; Wang, Yu; Xu, Aimin; Li, Shiwu; Liu, Pentao; Wu, Donghai

DOI: https://doi.org/10.1371/journal.pone.0075347

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Full title: Rational Design of a Fibroblast Growth Factor 21-Based Clinical Candidate, LY2405319

DOI: https://doi.org/10.1371/journal.pone.0058575

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Full title: Engineering the Expression and Characterization of Two Novel Laccase Isoenzymes from Coprinus comatus in Pichia pastoris by Fusing an Additional Ten Amino Acids Tag at N-Terminus

Authors: Chunjuan, Gu; Zheng, Fei; Long, Liangkun; Wang, Jing; Ding, Shaojun

DOI: https://doi.org/10.1371/journal.pone.0093912

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Full title: Fusing a Carbohydrate-Binding Module into the Aspergillus usamii β-Mannanase to Improve Its Thermostability and Cellulose-Binding Capacity by In Silico Design

Authors: Tang, Cunduo; Li, Jianfang; Wei, Xi-Huan; Min, Rou; Gao, Shujuan; Wang, Junqing; Yin, Xin; Wu, Minchen

DOI: https://doi.org/10.1371/journal.pone.0064766

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Expression and Purification of Integral Membrane Fatty Acid Desaturases

Chen, Haiqin; Gu, Zhennan; Zhang, Hao et al. · 2013

Full title: Expression and Purification of Integral Membrane Fatty Acid Desaturases

Authors: Chen, Haiqin; Gu, Zhennan; Zhang, Hao; Wang, Mingxuan; Chen, Wei; Lowther, W. Todd; Chen, Yong Q.

DOI: https://doi.org/10.1371/journal.pone.0058139

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Rocha, Saul Nitsche; Abrahão-Neto, José; Cerdán, M. Esperanza et al. · 2010

Full title: Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus

Authors: Rocha, Saul Nitsche; Abrahão-Neto, José; Cerdán, M. Esperanza; González-Siso, María-Isabel; Gombert, Andreas

DOI: https://doi.org/10.1186/1475-2859-9-4

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Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel

Huang, Jinjin; Xia, Ji; Yang, Zhen et al. · 2014

Full title: Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel

Authors: Huang, Jinjin; Xia, Ji; Yang, Zhen; Guan, Feifei; Cui, Di; Guan, Guohua; Jiang, Wei; Li, Ying

DOI: https://doi.org/10.1186/1754-6834-7-111

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Computational codon optimization of synthetic gene for protein expression

Chung, Bevan Kai-Sheng; Lee, Dong‐Yup · 2012

Full title: Computational codon optimization of synthetic gene for protein expression

Authors: Chung, Bevan Kai-Sheng; Lee, Dong‐Yup

DOI: https://doi.org/10.1186/1752-0509-6-134

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High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production

Yu, Xiao‐Wei; Sha, Chong; Guo, Yong-Liang et al. · 2013

Full title: High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production

Authors: Yu, Xiao‐Wei; Sha, Chong; Guo, Yong-Liang; Xiao, Rong; Xu, Yan

DOI: https://doi.org/10.1186/1754-6834-6-29

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Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice

Athmaram, TN; Saraswat, Shweta; Santhosh, SR et al. · 2011

Full title: Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice

Authors: Athmaram, TN; Saraswat, Shweta; Santhosh, SR; Singh, Ajay; Suryanarayana, VVS; Priya, Raj; Gopalan, Natarajan; Parida, Manmohan; Rao, PV Lakshmana; Vijayaraghavan, R.

DOI: https://doi.org/10.1186/1743-422x-8-524

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Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary 13C flux analysis

Jordà, Joel; Suarez-Mendez, Camilo; Carnicer, Marc et al. · 2013

Full title: Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary 13C flux analysis

Authors: Jordà, Joel; Suarez-Mendez, Camilo; Carnicer, Marc; Pierick, Angela ten; Heijnen, Joseph J.; Gulik, Walter van; Ferrer, Pau; Albiol, Joan; Wahl, A.

DOI: https://doi.org/10.1186/1752-0509-7-17

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Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica

Bulani, Siyavuya Ishmael; Moleleki, Lucy; Albertyn, Jacobus et al. · 2012

Full title: Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica

Authors: Bulani, Siyavuya Ishmael; Moleleki, Lucy; Albertyn, Jacobus; Moleleki, Ntsane

DOI: https://doi.org/10.1186/2191-0855-2-27

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High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

Ilmén, Marja; Haan, Riaan den; Brevnova, Elena E. et al. · 2011

Full title: High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

DOI: https://doi.org/10.1186/1754-6834-4-30

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Implications of cellobiohydrolase glycosylation for use in biomass conversion

Jeoh, Tina; Michener, William E.; Himmel, Michael E. et al. · 2008

Full title: Implications of cellobiohydrolase glycosylation for use in biomass conversion

Authors: Jeoh, Tina; Michener, William E.; Himmel, Michael E.; Decker, Stephen R.; Adney, William S.

DOI: https://doi.org/10.1186/1754-6834-1-10

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Construction of a novel Pichia pastoris strain for production of xanthophylls

Araya-Garay, José Miguel; Ageitos, José Manuel; Vallejo, Juán A. et al. · 2012

Full title: Construction of a novel Pichia pastoris strain for production of xanthophylls

Authors: Araya-Garay, José Miguel; Ageitos, José Manuel; Vallejo, Juán A.; Veiga‐Crespo, Patricia; Sánchez-Pérez, Ángeles; Villa, Tomás G.

DOI: https://doi.org/10.1186/2191-0855-2-24

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Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring

Abdulrachman, Dede; Thongkred, Paweena; Kocharin, Kanokarn et al. · 2017

Full title: Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring

DOI: https://doi.org/10.1186/s12896-017-0334-9

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Comparative genomics and transcriptomics of Pichia pastoris

M. Priest; Jonathan Borowsky; Sarah K. Young et al. · 2016

Full title: Comparative genomics and transcriptomics of Pichia pastoris

Authors: M. Priest; Jonathan Borowsky; Sarah K. Young; K. Love; Charles A. Whittaker; J. C. Love; Jie Wu; Duanduan Ma; Kartik Shah; M. C. Bartlett; Rachel Leeson

DOI: https://doi.org/10.1186/s12864-016-2876-y

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Genome-scale metabolic reconstructions of Pichia stipitis and Pichia pastoris and in silico evaluation of their potentials

Caspeta, Luis; Shoaie, Saeed; Ågren, Rasmus et al. · 2012

Full title: Genome-scale metabolic reconstructions of Pichia stipitis and Pichia pastoris and in silico evaluation of their potentials

Authors: Caspeta, Luis; Shoaie, Saeed; Ågren, Rasmus; Nookaew, Intawat; Nielsen, Jens

DOI: https://doi.org/10.1186/1752-0509-6-24

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Integrated lipase production and in situ biodiesel synthesis in a recombinant Pichia pastoris yeast: an efficient dual biocatalytic system composed of cell free enzymes and whole cell catalysts

Yan, Jinyong; Li, Zhaofeng; Du, Lei et al. · 2014

Authors: Yan, Jinyong; Li, Zhaofeng; Du, Lei; Li, Shengying

DOI: https://doi.org/10.1186/1754-6834-7-55

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A new acidophilic thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris

Liao, Hanpeng; Li, Shuixian; Zheng, Haiping et al. · 2014

Full title: A new acidophilic thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris

Authors: Liao, Hanpeng; Li, Shuixian; Zheng, Haiping; Wei, Zhong; Liu, Dongyang; Raza, Waseem; Shen, Qirong; Xu, Yangchun

DOI: https://doi.org/10.1186/s12896-014-0090-z

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Cloning and expression of Aspergillus flavus urate oxidase in Pichia pastoris

Fazel, Ramin; Zarei, Najmeh; Ghaemi, Nasser et al. · 2014

Full title: Cloning and expression of Aspergillus flavus urate oxidase in Pichia pastoris

Authors: Fazel, Ramin; Zarei, Najmeh; Ghaemi, Nasser; Namvaran, Mohammad Mehdi; Enayati, Somayeh; Mirabzadeh, Esmat; Azizi, Mohammad; Khalaj, Vahid

DOI: https://doi.org/10.1186/2193-1801-3-395

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Virus-like particles derived from Pichia pastoris-expressed dengue virus type 1 glycoprotein elicit homotypic virus-neutralizing envelope domain III-directed antibodies

Poddar, Ankur; Ramasamy, Viswanathan; Shukla, Rahul et al. · 2016

Full title: Virus-like particles derived from Pichia pastoris-expressed dengue virus type 1 glycoprotein elicit homotypic virus-neutralizing envelope domain III-directed antibodies

Authors: Poddar, Ankur; Ramasamy, Viswanathan; Shukla, Rahul; Rajpoot, Ravi Kant; Arora, Upasana; Jain, Swatantra Kumar; Swaminathan, S.; Khanna, Navin

DOI: https://doi.org/10.1186/s12896-016-0280-y

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Dynamic genome-scale metabolic modeling of the yeast Pichia pastoris

F. Saitua; Paulina Torres; J. Pérez-Correa et al. · 2017

Full title: Dynamic genome-scale metabolic modeling of the yeast Pichia pastoris

Authors: F. Saitua; Paulina Torres; J. Pérez-Correa; E. Agosin

DOI: https://doi.org/10.1186/s12918-017-0408-2

Paper

Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation

Shang, Tingting; Si, Dayong; Zhang, Dongyan et al. · 2017

Full title: Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation

Authors: Shang, Tingting; Si, Dayong; Zhang, Dongyan; Liu, Xuhui; Zhao, Longmei; Hu, Cong; Fu, Yu; Zhang, Rijun

DOI: https://doi.org/10.1186/s12896-017-0361-6

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An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)

Che, Zhiqun; Cao, Xiaoyan; Chen, Guiguang et al. · 2020

Full title: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)

Authors: Che, Zhiqun; Cao, Xiaoyan; Chen, Guiguang; Liang, Zhiqun

DOI: https://doi.org/10.1186/s12896-020-00654-7

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Expression and characteristics of manganese peroxidase from Ganoderma lucidum in Pichia pastoris and its application in the degradation of four dyes and phenol

Xu, Hui; Guo, Mengyuan; Gao, Yanhua et al. · 2017

Full title: Expression and characteristics of manganese peroxidase from Ganoderma lucidum in Pichia pastoris and its application in the degradation of four dyes and phenol

Authors: Xu, Hui; Guo, Mengyuan; Gao, Yanhua; Bai, Xiaohui; Zhou, Xuanwei

DOI: https://doi.org/10.1186/s12896-017-0338-5

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Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity

Yan, Guangbo; Min, Shu; Shen, Wei et al. · 2021

Full title: Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity

Authors: Yan, Guangbo; Min, Shu; Shen, Wei; Ma, Lixin; Zhai, Chao; Wang, Yaping; Huang, Zunxi

DOI: https://doi.org/10.1186/s12896-021-00708-4

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Casamino acids facilitate the secretion of recombinant dengue virus serotype-3 envelope domain III in Pichia pastoris

Kaushik, Neha; Rohila, Deepak; Arora, Upasana et al. · 2016

Full title: Casamino acids facilitate the secretion of recombinant dengue virus serotype-3 envelope domain III in Pichia pastoris

Authors: Kaushik, Neha; Rohila, Deepak; Arora, Upasana; Raut, Rajendra; Lamminmäki, Urpo; Khanna, Navin; Batra, Gaurav

DOI: https://doi.org/10.1186/s12896-016-0243-3

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Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris

Cheng Li; Ying-Wu Lin; Xueyun Zheng et al. · 2015

Full title: Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris

Authors: Cheng Li; Ying-Wu Lin; Xueyun Zheng; Nuo Pang; Xihao Liao; Xiaoxiao Liu; Yuanyuan Huang; Shuli Liang

DOI: https://doi.org/10.1186/s12896-015-0204-2

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Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Song, Xiaoping; Shao, Changsheng; Guo, Yugang et al. · 2019

Full title: Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Authors: Song, Xiaoping; Shao, Changsheng; Guo, Yugang; Wang, Yajie; Cai, Jingjing

DOI: https://doi.org/10.1186/s12896-019-0542-6

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Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks

Bawa, Zharain; Routledge, Sarah J; Jamshad, Mohammed et al. · 2014

Full title: Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks

Authors: Bawa, Zharain; Routledge, Sarah J; Jamshad, Mohammed; Clare, Michelle; Sarkar, Debasmita; Dickerson, Ian M.; Ganzlin, Markus; Poyner, David R.; Bill, Roslyn M.

DOI: https://doi.org/10.1186/s12934-014-0127-y

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Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

Chang, Ching-Hsiang; Hsiung, Hao-An; Hong, Kai‐Lin et al. · 2018

Full title: Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

Authors: Chang, Ching-Hsiang; Hsiung, Hao-An; Hong, Kai‐Lin; Huang, Ching‐Tsan

DOI: https://doi.org/10.1186/s12896-018-0492-4

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Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganoderma Species

Qu, Zhengwei; Zhou, Siya; Guan, Shixin et al. · 2018

Full title: Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganoderma Species

Authors: Qu, Zhengwei; Zhou, Siya; Guan, Shixin; Gao, Rui; Duan, Zuo-wen; Zhang, Xin; Sun, Wei-Yan; Fan, Wenli; Chen, Shuisen; Chen, Lijing; Lin, Jingwei; Ruan, Yanye

DOI: https://doi.org/10.1186/s12896-018-0488-0

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Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

Rüth, Claudia; Buchetics, Markus; Vidimce, Viktorija et al. · 2014

Full title: Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

Authors: Rüth, Claudia; Buchetics, Markus; Vidimce, Viktorija; Kotz, Daniela; Naschberger, Stefan; Mattanovich, Diethard; Pichler, Harald; Gasser, Brigitte

DOI: https://doi.org/10.1186/s12934-014-0120-5

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GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris

R. Prielhofer; Juan J. Barrero; Stefanie Steuer et al. · 2017

Full title: GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris

Authors: R. Prielhofer; Juan J. Barrero; Stefanie Steuer; T. Gassler; Richard J. Zahrl; Kristin Baumann; M. Sauer; D. Mattanovich; Brigitte Gasser; Hans Marx

DOI: https://doi.org/10.1186/s12918-017-0492-3

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Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation and by suppression of vacuolar targeting

Fitzgerald, Ivy; Glick, Benjamin S. · 2014

Full title: Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation and by suppression of vacuolar targeting

Authors: Fitzgerald, Ivy; Glick, Benjamin S.

DOI: https://doi.org/10.1186/s12934-014-0125-0

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Cell factories for insulin production

Baeshen, Nabih A.; Baeshen, Mohammed N.; Sheikh, Abdullah et al. · 2014

Full title: Cell factories for insulin production

Authors: Baeshen, Nabih A.; Baeshen, Mohammed N.; Sheikh, Abdullah; Bora, Roop Singh; Ahmed, Mohamed Morsi M.; Ramadan, Hassan A. I.; Saini, Kulvinder Singh; Redwan, Elrashdy M.

DOI: https://doi.org/10.1186/s12934-014-0141-0

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Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus

Yarimizu, Tohru; Nakamura, Mikiko; Hoshida, Hisashi et al. · 2015

Full title: Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus

Authors: Yarimizu, Tohru; Nakamura, Mikiko; Hoshida, Hisashi; Akada, Rinji

DOI: https://doi.org/10.1186/s12934-015-0203-y

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High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress and decreases production of human interleukin-10

Zhong, Yongjun; Yang, Lu; Guo, Yugang et al. · 2014

Full title: High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress and decreases production of human interleukin-10

Authors: Zhong, Yongjun; Yang, Lu; Guo, Yugang; Fang, Fang; Wang, Dong; Li, Rui; Jiang, Ming; Kang, Wenyao; Ma, Jiajia; Sun, Jie; Xiao, Weihua

DOI: https://doi.org/10.1186/s12934-014-0163-7

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Role of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichia pastoris

Yang, Min; Yu, Xiao‐Wei; Zheng, Haiyan et al. · 2015

Full title: Role of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichia pastoris

Authors: Yang, Min; Yu, Xiao‐Wei; Zheng, Haiyan; Sha, Chong; Zhao, Caifeng; Qian, Meiqian; Xu, Yan

DOI: https://doi.org/10.1186/s12934-015-0225-5

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Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems

Cristina, Pietro Della; Castagna, Monica; Lombardi, Alessio et al. · 2015

Full title: Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems

DOI: https://doi.org/10.1186/s12934-015-0202-z

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Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

Gmeiner, Christoph; Saadati, Amirhossein; Maresch, Daniel et al. · 2015

Full title: Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

Authors: Gmeiner, Christoph; Saadati, Amirhossein; Maresch, Daniel; Krasteva, Stanimira; Frank, Manuela; Altmann, Friedrich; Herwig, Christoph; Spadiut, Oliver

DOI: https://doi.org/10.1186/s12934-014-0183-3

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Construction of efficient xylose utilizing Pichia pastoris for industrial enzyme production

Pengfei Li; Hongbing Sun; Zao Chen et al. · 2015

Full title: Construction of efficient xylose utilizing Pichia pastoris for industrial enzyme production

Authors: Pengfei Li; Hongbing Sun; Zao Chen; Yin Li; T. Zhu

DOI: https://doi.org/10.1186/s12934-015-0206-8

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Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris

Vogl, Thomas; Ahmad, Mudassar; Krainer, Florian et al. · 2015

Full title: Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris

Authors: Vogl, Thomas; Ahmad, Mudassar; Krainer, Florian; Schwab, Helmut; Glieder, Anton

DOI: https://doi.org/10.1186/s12934-015-0293-6

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Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris

Florian W. Krainer; Simona Capone; Martin Jäger et al. · 2015

Full title: Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris

Authors: Florian W. Krainer; Simona Capone; Martin Jäger; T. Vogl; M. Gerstmann; A. Glieder; C. Herwig; O. Spadiut

DOI: https://doi.org/10.1186/s12934-014-0187-z

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Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs

Marco, Ario de · 2015

Full title: Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs

Authors: Marco, Ario de

DOI: https://doi.org/10.1186/s12934-015-0320-7

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ATP regulation in bioproduction

Hara, Kiyotaka Y.; Kondo, Akihiko · 2015

Full title: ATP regulation in bioproduction

Authors: Hara, Kiyotaka Y.; Kondo, Akihiko

DOI: https://doi.org/10.1186/s12934-015-0390-6

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Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli

Chen, Ana; Sun, Yang; Zhang, Wei et al. · 2016

Full title: Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli

Authors: Chen, Ana; Sun, Yang; Zhang, Wei; Peng, Feng; Zhan, Chunjun; Liu, Meng; Yang, Yankun; Bai, Zhonghu

DOI: https://doi.org/10.1186/s12934-015-0403-5

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Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter

Capone, Simona; Horvat, Jernej; Herwig, Christoph et al. · 2015

Full title: Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter

Authors: Capone, Simona; Horvat, Jernej; Herwig, Christoph; Spadiut, Oliver

DOI: https://doi.org/10.1186/s12934-015-0292-7

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Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology

Brigitte Gasser; Matthias G. Steiger; D. Mattanovich · 2015

Full title: Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology

Authors: Brigitte Gasser; Matthias G. Steiger; D. Mattanovich

DOI: https://doi.org/10.1186/s12934-015-0387-1

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The sweet branch of metabolic engineering: cherry-picking the low-hanging sugary fruits

Chen, Rachel · 2015

Full title: The sweet branch of metabolic engineering: cherry-picking the low-hanging sugary fruits

Authors: Chen, Rachel

DOI: https://doi.org/10.1186/s12934-015-0389-z

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Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris

Aw, Rochelle; Polizzi, Karen M. · 2016

Full title: Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris

Authors: Aw, Rochelle; Polizzi, Karen M.

DOI: https://doi.org/10.1186/s12934-016-0432-8

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Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization

Schwarzhans, Jan-Philipp; Wibberg, Daniel; Winkler, Anika et al. · 2016

Full title: Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization

Authors: Schwarzhans, Jan-Philipp; Wibberg, Daniel; Winkler, Anika; Luttermann, Tobias; Kalinowski, Jörn; Friehs, Karl

DOI: https://doi.org/10.1186/s12934-016-0486-7

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Enhancing antibody folding and secretion by tailoring the Saccharomyces cerevisiae endoplasmic reticulum

Ruijter, Jorg C. de; Koskela, Essi V.; Frey, Alexander D. · 2016

Full title: Enhancing antibody folding and secretion by tailoring the Saccharomyces cerevisiae endoplasmic reticulum

Authors: Ruijter, Jorg C. de; Koskela, Essi V.; Frey, Alexander D.

DOI: https://doi.org/10.1186/s12934-016-0488-5

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A high-throughput expression screening platform to optimize the production of antimicrobial peptides

Schreiber, Christine; Müller, Hagen; Birrenbach, Oliver et al. · 2017

Full title: A high-throughput expression screening platform to optimize the production of antimicrobial peptides

Authors: Schreiber, Christine; Müller, Hagen; Birrenbach, Oliver; Klein, Moritz; Heerd, Doreen; Weidner, Tobias; Salzig, Denise; Czermak, Peter

DOI: https://doi.org/10.1186/s12934-017-0637-5

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Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica

Sassi, Hosni; Delvigne, Frank; Kar, Tambi et al. · 2016

Full title: Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica

Authors: Sassi, Hosni; Delvigne, Frank; Kar, Tambi; Nicaud, Jean‐Marc; Coq, Anne-Marie Crutz-Le; Steels, Sébastien; Fickers, Patrick

DOI: https://doi.org/10.1186/s12934-016-0558-8

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A novel methanol-free Pichia pastoris system for recombinant protein expression

Shen, Wei; Xue, Ying; Liu, Yiqi et al. · 2016

Full title: A novel methanol-free Pichia pastoris system for recombinant protein expression

Authors: Shen, Wei; Xue, Ying; Liu, Yiqi; Kong, Chuixing; Wang, Xiaolong; Huang, Mengmeng; Cai, Menghao; Zhou, Xiangshan; Zhang, Yuanxing; Zhou, Mian

DOI: https://doi.org/10.1186/s12934-016-0578-4

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Functional inclusion bodies produced in the yeast Pichia pastoris

Rueda, Fabián; Gasser, Brigitte; Sánchez‐Chardi, Alejandro et al. · 2016

Full title: Functional inclusion bodies produced in the yeast Pichia pastoris

DOI: https://doi.org/10.1186/s12934-016-0565-9

Paper

Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae

Xu, Sha; Geyuan, Zhang; Zhang, Huijie et al. · 2016

Full title: Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae

Authors: Xu, Sha; Geyuan, Zhang; Zhang, Huijie; Kitajima, Toshihiko; Nakanishi, Hideki; Gao, Xiao‐Dong

DOI: https://doi.org/10.1186/s12934-016-0575-7

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Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source

Schotte, Peter; Dewerte, Isabelle; Groeve, Manu De et al. · 2016

Full title: Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source

Authors: Schotte, Peter; Dewerte, Isabelle; Groeve, Manu De; Keyser, Saskia De; Brabandere, Veronique De; Stanssens, Patrick

DOI: https://doi.org/10.1186/s12934-016-0499-2

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A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis

Cai, Dongbo; Wang, Hao; He, Penghui et al. · 2017

Full title: A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis

Authors: Cai, Dongbo; Wang, Hao; He, Penghui; Zhu, Chengjun; Wang, Qin; Wei, Xuetuan; Nomura, Christopher T.; Chen, Shouwen

DOI: https://doi.org/10.1186/s12934-017-0688-7

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Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

Camattari, Andrea; Goh, Amelia; Yip, Lian Yee et al. · 2016

Full title: Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

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Full title: Expression and characterization of a novel 1,3-regioselective cold-adapted lipase from Rhizomucor endophyticus suitable for biodiesel synthesis

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Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris

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Full title: Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris

Authors: Yuan, Huiling; Zhou, Ying; Lin, Yuping; Tu, Ran; Guo, Yufeng; Zhang, Yuanyuan; Wang, Qinhong

DOI: https://doi.org/10.1186/s13068-022-02150-w

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Characterization and modulation of endoplasmic reticulum stress response target genes in Kluyveromyces marxianus to improve secretory expressions of heterologous proteins

Shi, Tianfang; Zhou, Jungang; Xue, Aijuan et al. · 2021

Full title: Characterization and modulation of endoplasmic reticulum stress response target genes in Kluyveromyces marxianus to improve secretory expressions of heterologous proteins

Authors: Shi, Tianfang; Zhou, Jungang; Xue, Aijuan; Lü, Hong; He, Yungang; Yu, Yao

DOI: https://doi.org/10.1186/s13068-021-02086-7

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Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design

Li, Xueqing; Wu, Qin; Hu, Die et al. · 2017

Full title: Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design

Authors: Li, Xueqing; Wu, Qin; Hu, Die; Wang, Rui; Liu, Yan; Wu, Minchen; Li, Jianfang

DOI: https://doi.org/10.1186/s13568-017-0399-9

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Characterization of recombinant laccase from Trametes versicolor synthesized by Arxula adeninivorans and its application in the degradation of pharmaceuticals

Litwińska, Katarzyna; Bischoff, Felix; Matthes, Falko et al. · 2019

Full title: Characterization of recombinant laccase from Trametes versicolor synthesized by Arxula adeninivorans and its application in the degradation of pharmaceuticals

Authors: Litwińska, Katarzyna; Bischoff, Felix; Matthes, Falko; Bode, Rüdiger; Rutten, Twan; Kunze, Gotthard

DOI: https://doi.org/10.1186/s13568-019-0832-3

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Deciphering cell wall sensors enabling the construction of robust P. pastoris for single-cell protein production

Gao, Le; Meng, Jiao; Dai, Wuling et al. · 2023

Full title: Deciphering cell wall sensors enabling the construction of robust P. pastoris for single-cell protein production

Authors: Gao, Le; Meng, Jiao; Dai, Wuling; Zhang, Zhaokun; Dong, Haofan; Yuan, Qianqian; Zhang, Wuyuan; Liu, Shuguang; Wu, Xin

DOI: https://doi.org/10.1186/s13068-023-02428-7

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Improvement of efficiency of brown coal biosolubilization by novel recombinant Fusarium oxysporum laccase

Kwiatos, Natalia; Jędrzejczak‐Krzepkowska, Marzena; Strzelecki, Bartosz et al. · 2018

Full title: Improvement of efficiency of brown coal biosolubilization by novel recombinant Fusarium oxysporum laccase

Authors: Kwiatos, Natalia; Jędrzejczak‐Krzepkowska, Marzena; Strzelecki, Bartosz; Bielecki, Stanisław

DOI: https://doi.org/10.1186/s13568-018-0669-1

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The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella phaffii

Zou, Chenwei; Lu, Lingfang; Wang, Shengyan et al. · 2022

Full title: The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella phaffii

Authors: Zou, Chenwei; Lu, Lingfang; Wang, Shengyan; Zhang, Chenshan; Chen, Xuequn; Lin, Yao; Huang, Yide

DOI: https://doi.org/10.1186/s13068-022-02243-6

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The first fungal laccase with an alkaline pH optimum obtained by directed evolution and its application in indigo dye decolorization

Yin, Qiang; Zhou, Gang; Peng, Can et al. · 2019

Full title: The first fungal laccase with an alkaline pH optimum obtained by directed evolution and its application in indigo dye decolorization

Authors: Yin, Qiang; Zhou, Gang; Peng, Can; Zhang, Yinliang; Kües, Ursula; Liu, Juanjuan; Xiao, Yazhong; Fang, Zemin

DOI: https://doi.org/10.1186/s13568-019-0878-2

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Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila

Bonzom, Cyrielle; Hüttner, Silvia; Mirgorodskaya, Ekaterina et al. · 2019

Full title: Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila

Authors: Bonzom, Cyrielle; Hüttner, Silvia; Mirgorodskaya, Ekaterina; Chong, Sun‐Li; Uthoff, Stefan; Steinbüchel, Alexander; Verhaert, R.M.D.; Olsson, Lisbeth

DOI: https://doi.org/10.1186/s13568-019-0852-z

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Improved methanol-derived lovastatin production through enhancement of the biosynthetic pathway and intracellular lovastatin efflux in methylotrophic yeast

Liu, Yiqi; Bai, Chenxiao; Xu, Qin et al. · 2018

Full title: Improved methanol-derived lovastatin production through enhancement of the biosynthetic pathway and intracellular lovastatin efflux in methylotrophic yeast

Authors: Liu, Yiqi; Bai, Chenxiao; Xu, Qin; Yu, Jiahui; Zhou, Xiangshan; Zhang, Yuanxing; Cai, Menghao

DOI: https://doi.org/10.1186/s40643-018-0202-z

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Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris

Vogl, Thomas; Fischer, Jasmin Elgin; Hyden, Patrick et al. · 2020

Full title: Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris

Authors: Vogl, Thomas; Fischer, Jasmin Elgin; Hyden, Patrick; Wasmayer, Richard; Sturmberger, Lukas; Glieder, Anton

DOI: https://doi.org/10.1186/s13568-020-00972-1

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Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA:ethanol O-acyltransferase Eht1 or Eeb1

Zhuang, Shiwen; Fu, Junshu; Powell, Chris et al. · 2015

Authors: Zhuang, Shiwen; Fu, Junshu; Powell, Chris; Huang, Jinhai; Xia, Yihe; Yan, Ruixiang

DOI: https://doi.org/10.1186/s40064-015-1195-0

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Transcriptional regulatory networks of methanol-independent protein expression in Pichia pastoris under the AOX1 promoter with trans-acting elements engineering

Lei Shi; Jinjia Wang; Xiaolong Wang et al. · 2020

Full title: Transcriptional regulatory networks of methanol-independent protein expression in Pichia pastoris under the AOX1 promoter with trans-acting elements engineering

Authors: Lei Shi; Jinjia Wang; Xiaolong Wang; Yuanxing Zhang; Zhiwei Song; Menghao Cai; Xiangshan Zhou

DOI: https://doi.org/10.1186/s40643-020-00306-w

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Characterization, expression and application of a zearalenone degrading enzyme from Neurospora crassa

Bi, Ke; Zhang, Wen; Xiao, Zhizhuang et al. · 2018

Full title: Characterization, expression and application of a zearalenone degrading enzyme from Neurospora crassa

Authors: Bi, Ke; Zhang, Wen; Xiao, Zhizhuang; Zhang, Dawei

DOI: https://doi.org/10.1186/s13568-018-0723-z

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Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris

Ma, Lingling; Liang, Xiaolin; Yu, Shiqin et al. · 2022

Full title: Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pastoris

Authors: Ma, Lingling; Liang, Xiaolin; Yu, Shiqin; Zhou, Jingwen

DOI: https://doi.org/10.1186/s40643-022-00606-3

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High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris

Zhi-Xiang Xiang; Jin-Song Gong; Jin-Hao Shi et al. · 2022

Full title: High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris

Authors: Zhi-Xiang Xiang; Jin-Song Gong; Jin-Hao Shi; Chun-Fang Liu; Heng Li; Chang Su; Min Jiang; Zhenghong Xu; Jin-song Shi

DOI: https://doi.org/10.1186/s40643-022-00605-4

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Development of a Pichia pastoris cell factory for efficient production of germacrene A: a precursor of β-elemene

Cheng, Jintao; Zuo, Yimeng; Liu, Gaofei et al. · 2023

Full title: Development of a Pichia pastoris cell factory for efficient production of germacrene A: a precursor of β-elemene

Authors: Cheng, Jintao; Zuo, Yimeng; Liu, Gaofei; Li, Dongfang; Gao, Jucan; Xiao, Feng; Huang, Lei; Xu, Zhinan; Lian, Jiazhang

DOI: https://doi.org/10.1186/s40643-023-00657-0

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Human LYPD8 protein inhibits motility of flagellated bacteria

Hsu, Chiao-Ching; Okumura, Ryu; Takeda, Kiyoshi · 2017

Full title: Human LYPD8 protein inhibits motility of flagellated bacteria

Authors: Hsu, Chiao-Ching; Okumura, Ryu; Takeda, Kiyoshi

DOI: https://doi.org/10.1186/s41232-017-0056-3

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From induction to secretion: a complicated route for cellulase production in Trichoderma reesei

Su, Yan; Xu, Yan; Yu, Xiao‐Wei · 2021

Full title: From induction to secretion: a complicated route for cellulase production in Trichoderma reesei

Authors: Su, Yan; Xu, Yan; Yu, Xiao‐Wei

DOI: https://doi.org/10.1186/s40643-021-00461-8

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Stabilisation of the Fc Fragment of Human IgG1 by Engineered Intradomain Disulfide Bonds

Wozniak‐Knopp, Gordana; Stadlmann, Johannes; Rüker, Florian · 2012

Full title: Stabilisation of the Fc Fragment of Human IgG1 by Engineered Intradomain Disulfide Bonds

Authors: Wozniak‐Knopp, Gordana; Stadlmann, Johannes; Rüker, Florian

DOI: https://doi.org/10.1371/journal.pone.0030083

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Secretory expression of thermostable alkaline protease from Bacillus stearothermophilus FI by using native signal peptide and α -factor secretion signal in Pichia pastoris

Latiffi, Amaliawati Ahmad; Salleh, Abu Bakar; Rahman, Raja Noor Zaliha Raja Abd et al. · 2013

Full title: Secretory expression of thermostable alkaline protease from Bacillus stearothermophilus FI by using native signal peptide and α -factor secretion signal in Pichia pastoris

Authors: Latiffi, Amaliawati Ahmad; Salleh, Abu Bakar; Rahman, Raja Noor Zaliha Raja Abd; Oslan, Siti Nurbaya; Basri, Mahiran

DOI: https://doi.org/10.1266/ggs.88.85

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A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris

Bai, Jiangping; Swartz, Douglas J.; Protasevich, Irina I. et al. · 2011

Full title: A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris

DOI: https://doi.org/10.1371/journal.pone.0022577

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Native-State Stability Determines the Extent of Degradation Relative to Secretion of Protein Variants from Pichia pastoris

G. Whyteside; M. Alcocer; J. Kumita et al. · 2011

Full title: Native-State Stability Determines the Extent of Degradation Relative to Secretion of Protein Variants from Pichia pastoris

Authors: G. Whyteside; M. Alcocer; J. Kumita; C. Dobson; M. Lazarou; R. Pleass; D. Archer

DOI: https://doi.org/10.1371/journal.pone.0022692

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Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity.

Love, Kerry Routenberg; Politano, Timothy J; Panagiotou, Vasiliki et al. · 2012

Full title: Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity.

Authors: Love, Kerry Routenberg; Politano, Timothy J; Panagiotou, Vasiliki; Jiang, Bo; Stadheim, Terrance A; Love, J Christopher

DOI: https://doi.org/10.1371/journal.pone.0037915

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Amino Acid Substitutions at the Major Insertion Loop of Candida albicans Sterol 14alpha-Demethylase Are Involved in Fluconazole Resistance

Alvarez-Rueda, Nidia; Fleury, Audrey; Morio, Florent et al. · 2011

Full title: Amino Acid Substitutions at the Major Insertion Loop of Candida albicans Sterol 14alpha-Demethylase Are Involved in Fluconazole Resistance

Authors: Alvarez-Rueda, Nidia; Fleury, Audrey; Morio, Florent; Pagniez, Fabrice; Gastinel, Louis N.; Pape, Patrice Le

DOI: https://doi.org/10.1371/journal.pone.0021239

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Efficient protein production by yeast requires global tuning of metabolism

Huang, Mingtao; Bao, Jichen; Hallström, Björn M. et al. · 2017

Full title: Efficient protein production by yeast requires global tuning of metabolism

Authors: Huang, Mingtao; Bao, Jichen; Hallström, Björn M.; Petranović, Dina; Nielsen, Jens

DOI: https://doi.org/10.1038/s41467-017-00999-2

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Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care

Pérez‐Piñera, Pablo; Han, Ningren; Cleto, Sara et al. · 2016

Full title: Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care

Authors: Pérez‐Piñera, Pablo; Han, Ningren; Cleto, Sara; Cao, Jicong; Purcell, Oliver; Shah, Kartik; Lee, Kevin; Ram, Rajeev J.; Lu, Timothy K.

DOI: https://doi.org/10.1038/ncomms12211

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Genome sequence of the recombinant protein production host Pichia pastoris

Schutter, Kristof De; Lin, Yao‐Cheng; Tiels, Petra et al. · 2009

Full title: Genome sequence of the recombinant protein production host Pichia pastoris

Authors: Schutter, Kristof De; Lin, Yao‐Cheng; Tiels, Petra; Hecke, Annelies Van; Glinka, Sascha; Weber-Lehmann, Jacqueline; Rouzé, Pierre; Peer, Yves Van de; Callewaert, Nico

DOI: https://doi.org/10.1038/nbt.1544

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Versatile and on-demand biologics co-production in yeast

Cao, Jicong; Pérez‐Piñera, Pablo; Lowenhaupt, Ky et al. · 2018

Full title: Versatile and on-demand biologics co-production in yeast

Authors: Cao, Jicong; Pérez‐Piñera, Pablo; Lowenhaupt, Ky; Wu, Ming-Ru; Purcell, Oliver; Fuente‐Núñez, César de la; Lu, Timothy K.

DOI: https://doi.org/10.1038/s41467-017-02587-w

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RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes

Abatemarco, Joseph; Sarhan, Maen F.; Wagner, James M. et al. · 2017

Full title: RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes

Authors: Abatemarco, Joseph; Sarhan, Maen F.; Wagner, James M.; Lin, Jyun‐Liang; Liu, Leqian; Hassouneh, Wafa; Yuan, Shuo‐Fu; Alper, Hal S.; Abate, Adam R.

DOI: https://doi.org/10.1038/s41467-017-00425-7

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Engineering consortia by polymeric microbial swarmbots

Wang, Lin; Zhang, Xi; Tang, Chenwang et al. · 2022

Full title: Engineering consortia by polymeric microbial swarmbots

DOI: https://doi.org/10.1038/s41467-022-31467-1

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A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast

Naseri, Gita · 2023

Full title: A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast

Authors: Naseri, Gita

DOI: https://doi.org/10.1038/s41467-023-37627-1

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A Cas3-base editing tool for targetable in vivo mutagenesis

Zimmermann, Anna; Prieto-Vivas, Julian E.; Cautereels, Charlotte et al. · 2023

Full title: A Cas3-base editing tool for targetable in vivo mutagenesis

Authors: Zimmermann, Anna; Prieto-Vivas, Julian E.; Cautereels, Charlotte; Gorkovskiy, Anton; Steensels, Jan; Peer, Yves Van de; Verstrepen, Kevin J.

DOI: https://doi.org/10.1038/s41467-023-39087-z

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Improving recombinant protein production by yeast through genome-scale modeling using proteome constraints

Li, Feiran; Chen, Yu; Qi, Qi et al. · 2022

Full title: Improving recombinant protein production by yeast through genome-scale modeling using proteome constraints

Authors: Li, Feiran; Chen, Yu; Qi, Qi; Wang, Yanyan; Yuan, Le; Huang, Mingtao; Elsemman, Ibrahim E.; Feizi, Amir; Kerkhoven, Eduard J.; Nielsen, Jens

DOI: https://doi.org/10.1038/s41467-022-30689-7

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Engineered bidirectional promoters enable rapid multi-gene co-expression optimization

T. Vogl; Thomas Kickenweiz; Julia Pitzer et al. · 2018

Full title: Engineered bidirectional promoters enable rapid multi-gene co-expression optimization

DOI: https://doi.org/10.1038/s41467-018-05915-w

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A polycistronic system for multiplexed and precalibrated expression of multigene pathways in fungi

Yue, Qun; Meng, Jie; Qiu, Yue et al. · 2023

Full title: A polycistronic system for multiplexed and precalibrated expression of multigene pathways in fungi

DOI: https://doi.org/10.1038/s41467-023-40027-0

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Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris

Cheng, Li; Lin, Ying; Zheng, Xueyun et al. · 2017

Full title: Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris

Authors: Cheng, Li; Lin, Ying; Zheng, Xueyun; Yuan, Qingyan; Pang, Nuo; Liao, Xihao; Huang, Yuanyuan; Zhang, Xinying; Liang, Shuli

DOI: https://doi.org/10.1038/s41598-017-11494-5

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Immobilized enzyme cascade for targeted glycosylation

Makrydaki, Elli; Donini, Roberto; Krueger, A. P. et al. · 2024

Full title: Immobilized enzyme cascade for targeted glycosylation

Authors: Makrydaki, Elli; Donini, Roberto; Krueger, A. P.; Royle, Kate E.; Moya‐Ramírez, Ignacio; Kuntz, D.A.; Rose, David R.; Haslam, Stuart M.; Polizzi, Karen M.; Kontoravdi, Cleo

DOI: https://doi.org/10.1038/s41589-023-01539-4

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Controlling AOX1 promoter strength in Pichia pastoris by manipulating poly (dA:dT) tracts

Jun Yang; Haiming Cai; Jie Liu et al. · 2018

Full title: Controlling AOX1 promoter strength in Pichia pastoris by manipulating poly (dA:dT) tracts

Authors: Jun Yang; Haiming Cai; Jie Liu; Min Zeng; Jiawei Chen; Qingmei Cheng; Linghua Zhang

DOI: https://doi.org/10.1038/s41598-018-19831-y

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An improved pathway for autonomous bioluminescence imaging in eukaryotes

Shakhova, Ekaterina S.; Karataeva, Tatiana A.; Markina, Nadezhda M. et al. · 2024

Full title: An improved pathway for autonomous bioluminescence imaging in eukaryotes

DOI: https://doi.org/10.1038/s41592-023-02152-y

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High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin

Chen, Ximing; Li, Juan; Sun, Haili et al. · 2017

Full title: High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich antibacterial honeybee peptide Apidaecin

Authors: Chen, Ximing; Li, Juan; Sun, Haili; Li, Shi-Weng; Chen, Tuo; Liu, Guangxiu; Dyson, Paul

DOI: https://doi.org/10.1038/s41598-017-15149-3

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OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression

Claes, Katrien; Herpe, Dries Van; Vanluchene, Robin et al. · 2024

Full title: OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression

DOI: https://doi.org/10.1038/s41564-023-01574-w

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Efficient production and characterization of the novel and highly active antifungal protein AfpB from Penicillium digitatum

Garrigues, Sandra; Gandía, Mónica; Popa, Crina et al. · 2017

Full title: Efficient production and characterization of the novel and highly active antifungal protein AfpB from Penicillium digitatum

Authors: Garrigues, Sandra; Gandía, Mónica; Popa, Crina; Borics, Attila; Marx, Florentine; Coca, María; Marcos, José F.; Manzanares, Paloma

DOI: https://doi.org/10.1038/s41598-017-15277-w

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Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects

Yu, Xiao‐Wei; Sun, Weihong; Wang, Ying-Zheng et al. · 2017

Full title: Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects

Authors: Yu, Xiao‐Wei; Sun, Weihong; Wang, Ying-Zheng; Xu, Yan

DOI: https://doi.org/10.1038/s41598-017-16577-x

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Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B

Theron, Chrispian W.; Vandermies, Marie; Telek, Samuel et al. · 2020

Full title: Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B

Authors: Theron, Chrispian W.; Vandermies, Marie; Telek, Samuel; Steels, Sébastien; Fickers, Patrick

DOI: https://doi.org/10.1038/s41598-020-58683-3

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The effect of non-thermal atmospheric plasma on the production and activity of recombinant phytase enzyme

Farasat, Mahsa; Arjmand, Sareh; Siadat, Seyed Omid Ranaei et al. · 2018

Full title: The effect of non-thermal atmospheric plasma on the production and activity of recombinant phytase enzyme

Authors: Farasat, Mahsa; Arjmand, Sareh; Siadat, Seyed Omid Ranaei; Sefidbakht, Yahya; Ghomi, Hamid

DOI: https://doi.org/10.1038/s41598-018-34239-4

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Evolving stability and pH-dependent activity of the high redox potential Botrytis aclada laccase for enzymatic fuel cells

Scheiblbrandner, Stefan; Breslmayr, Erik; Csarman, Florian et al. · 2017

Full title: Evolving stability and pH-dependent activity of the high redox potential Botrytis aclada laccase for enzymatic fuel cells

DOI: https://doi.org/10.1038/s41598-017-13734-0

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Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells

Arbeitman, Claudia R.; Auge, Gabriela; Blaustein, Matı́as et al. · 2020

Full title: Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells

DOI: https://doi.org/10.1038/s41598-020-78711-6

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Improved thermostability of an acidic xylanase from Aspergillus sulphureus by combined disulphide bridge introduction and proline residue substitution

Yang, Wenhan; Yang, Yongzhi; Zhang, Lingdi et al. · 2017

Full title: Improved thermostability of an acidic xylanase from Aspergillus sulphureus by combined disulphide bridge introduction and proline residue substitution

Authors: Yang, Wenhan; Yang, Yongzhi; Zhang, Lingdi; Xu, Hang; Guo, Xiaojing; Yang, Xu; Dong, Bing; Cao, Yunhe

DOI: https://doi.org/10.1038/s41598-017-01758-5

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FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mammalian Reporter Cells

Yanakieva, Desislava; Elter, Adrian; Bratsch, Jens et al. · 2020

Full title: FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mammalian Reporter Cells

Authors: Yanakieva, Desislava; Elter, Adrian; Bratsch, Jens; Friedrich, Karlheinz; Becker, Stefan; Kolmar, Harald

DOI: https://doi.org/10.1038/s41598-020-66927-5

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Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clones in microscale

Kaushik, Neha; Lamminmäki, Urpo; Khanna, Navin et al. · 2020

Full title: Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clones in microscale

Authors: Kaushik, Neha; Lamminmäki, Urpo; Khanna, Navin; Batra, Gaurav

DOI: https://doi.org/10.1038/s41598-020-63995-5

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Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

Binyang Deng; Y. Yue; Jun Yang et al. · 2023

Full title: Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

Authors: Binyang Deng; Y. Yue; Jun Yang; Mingjun Yang; Qiong Xing; Hang Peng; Fei Wang; Ming Li; Lixin Ma; C. Zhai

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High-yield novel leech hyaluronidase to expedite the preparation of specific hyaluronan oligomers

Jin, Peng; Kang, Zhen; Zhang, Na et al. · 2014

Full title: High-yield novel leech hyaluronidase to expedite the preparation of specific hyaluronan oligomers

Authors: Jin, Peng; Kang, Zhen; Zhang, Na; Du, Guocheng; Chen, Jian

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Cold atmospheric plasma treatment enhances recombinant model protein production in yeast Pichia pastoris

Zeinab Kabarkouhi; S. Arjmand; Seyed Omid Ranaei Siadat et al. · 2023

Full title: Cold atmospheric plasma treatment enhances recombinant model protein production in yeast Pichia pastoris

Authors: Zeinab Kabarkouhi; S. Arjmand; Seyed Omid Ranaei Siadat; B. Shokri

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Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris

Yu, Ping; Yan, Yuan; Gu, Qing et al. · 2013

Full title: Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris

Authors: Yu, Ping; Yan, Yuan; Gu, Qing; Wang, Xiangyang

DOI: https://doi.org/10.1038/srep03043

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Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement

Liu, Wan-Cang; Gong, Ting; Wang, Qinghua et al. · 2016

Full title: Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement

Authors: Liu, Wan-Cang; Gong, Ting; Wang, Qinghua; Liang, Xiao; Chen, Jingjing; Zhu, Ping

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Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts

Schütz, Marco; Schöppe, Jendrik; Sedlák, Erik et al. · 2016

Full title: Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts

Authors: Schütz, Marco; Schöppe, Jendrik; Sedlák, Erik; Hillenbrand, Matthias; Nagy‐Davidescu, Gabriela; Ehrenmann, Janosch; Klenk, Christoph; Egloff, Pascal; Kummer, Lutz; Plückthun, Andreas

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An Efficient Genome-Wide Fusion Partner Screening System for Secretion of Recombinant Proteins in Yeast

Bae, Jung-Hoon; Sung, Bong Hyun; Kim, Hyun‐Jin et al. · 2015

Full title: An Efficient Genome-Wide Fusion Partner Screening System for Secretion of Recombinant Proteins in Yeast

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Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris

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Full title: Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris

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Full title: Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris

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Full title: A collagen-binding EGFR antibody fragment targeting tumors with a collagen-rich extracellular matrix

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Full title: Functional diversity and properties of multiple xylanases from Penicillium oxalicum GZ-2

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Full title: Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris

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Rodríguez‐López, Alexander; Alméciga-Díaz, Carlos Javier; Sánchez, Jhonnathan et al. · 2016

Full title: Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastoris

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Full title: Enhanced and homogeneous oxygen availability during incubation of microfluidic droplets

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Full title: Multi-level glyco-engineering techniques to generate IgG with defined Fc-glycans

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Full title: Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy

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Lu, Yihong; Fang, Cheng; Wang, Qinhong et al. · 2016

Full title: High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation

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Full title: A Review of Xylanase Production by the Fermentation of Xylan: Classification, Characterization and Applications

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Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications

Parashar, Deepak; Satyanarayana, T. · 2017

Full title: Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications

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DOI: https://doi.org/10.3389/fmicb.2017.00493

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A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris

Li, Ding; Zhang, Bo; Li, Shu‐Ting et al. · 2017

Full title: A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris

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DOI: https://doi.org/10.3389/fmicb.2017.01698

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Pichia pastoris-Expressed Bivalent Virus-Like Particulate Vaccine Induces Domain III-Focused Bivalent Neutralizing Antibodies without Antibody-Dependent Enhancement in Vivo

Shukla, Rahul; Rajpoot, Ravi Kant; Arora, Upasana et al. · 2018

Full title: Pichia pastoris-Expressed Bivalent Virus-Like Particulate Vaccine Induces Domain III-Focused Bivalent Neutralizing Antibodies without Antibody-Dependent Enhancement in Vivo

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DOI: https://doi.org/10.3389/fmicb.2017.02644

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Enzymatic Degradation of Aromatic and Aliphatic Polyesters by P. pastoris Expressed Cutinase 1 from Thermobifida cellulosilytica

Gamerith, Caroline; Vastano, Marco; Ghorbanpour, Sahar et al. · 2017

Full title: Enzymatic Degradation of Aromatic and Aliphatic Polyesters by P. pastoris Expressed Cutinase 1 from Thermobifida cellulosilytica

DOI: https://doi.org/10.3389/fmicb.2017.00938

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Systematic Optimization of Protein Secretory Pathways in Saccharomyces cerevisiae to Increase Expression of Hepatitis B Small Antigen

Sheng, Jiayuan; Flick, Hunter; Feng, Xueyang · 2017

Full title: Systematic Optimization of Protein Secretory Pathways in Saccharomyces cerevisiae to Increase Expression of Hepatitis B Small Antigen

Authors: Sheng, Jiayuan; Flick, Hunter; Feng, Xueyang

DOI: https://doi.org/10.3389/fmicb.2017.00875

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Pichia pastoris-expressed dengue 3 envelope-based virus-like particles elicit predominantly domain III-focused high titer neutralizing antibodies

Tripathi, Lav; Mani, Shailendra; Raut, Rajendra et al. · 2015

Full title: Pichia pastoris-expressed dengue 3 envelope-based virus-like particles elicit predominantly domain III-focused high titer neutralizing antibodies

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DOI: https://doi.org/10.3389/fmicb.2015.01005

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Four Inducible Promoters for Controlled Gene Expression in the Oleaginous Yeast Rhodotorula toruloides

Johns, Alexander; Love, John; Aves, Stephen J. · 2016

Full title: Four Inducible Promoters for Controlled Gene Expression in the Oleaginous Yeast Rhodotorula toruloides

Authors: Johns, Alexander; Love, John; Aves, Stephen J.

DOI: https://doi.org/10.3389/fmicb.2016.01666

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Liu, Wan-Cang; Xiang, Haibo; Zhang, Tao et al. · 2020

Full title: Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia pastoris

Authors: Liu, Wan-Cang; Xiang, Haibo; Zhang, Tao; Pang, Xu; Su, Jing; Liu, Hongyu; Ma, Baiping; Yu, Liyan

DOI: https://doi.org/10.3389/fmicb.2020.01988

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Full title: Single-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes With Pichia pastoris

Authors: Hana Raschmanová; Iwo Zamora; Martina Borčinová; Patrick Meier; A. Weninger; Dominik Mächler; A. Glieder; K. Melzoch; Z. Knejzlík; K. Kovar

DOI: https://doi.org/10.3389/fmicb.2019.00335

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Full title: Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide in Pichia pastoris

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DOI: https://doi.org/10.3389/fmicb.2019.01472

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Full title: Komagataella phaffii as Emerging Model Organism in Fundamental Research.

Authors: Bernauer, Lukas; Radkohl, Astrid; Lehmayer, Leonie Gabriela Katharina; Emmerstorfer-Augustin, Anita

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Joseph, Jewel Ann; Akkermans, Simen; Nimmegeers, Philippe et al. · 2019

Full title: Bioproduction of the Recombinant Sweet Protein Thaumatin: Current State of the Art and Perspectives

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Full title: Insight to Improve α-L-Arabinofuranosidase Productivity in Pichia pastoris and Its Application on Corn Stover Degradation

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Full title: Sophisticated Cloning, Fermentation, and Purification Technologies for an Enhanced Therapeutic Protein Production: A Review

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Full title: Current advances of Pichia pastoris as cell factories for production of recombinant proteins

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Full title: Molecular Modification of Kex2 P1’ Site Enhances Expression and Druggability of Fungal Defensin

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Dadwal, Anica; Sharma, Shilpa; Satyanarayana, T. · 2020

Full title: Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering Approaches for Cellulose Saccharification

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Full title: Strategies for Optimizing the Production of Proteins and Peptides with Multiple Disulfide Bonds

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Full title: Comparative transcriptomic analysis-based identification of the regulation of foreign proteins with different stabilities expressed in Pichia pastoris.

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Full title: A Valuable Product of Microbial Cell Factories: Microbial Lipase

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Chen, Xinyi; Li, Chun; Liu, Hu · 2021

Full title: Enhanced Recombinant Protein Production Under Special Environmental Stress

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Benedetti, Manuel; Locci, Federica; Gramegna, Giovanna et al. · 2019

Full title: Green Production and Biotechnological Applications of Cell Wall Lytic Enzymes

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Full title: Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii

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Maity, Nitu; Mishra, Saroj · 2019

Full title: Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris.

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Kuroda, Kouichi; Ueda, Mitsuyoshi · 2013

Full title: Arming Technology in Yeast—Novel Strategy for Whole-cell Biocatalyst and Protein Engineering

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Pouzet, Sylvain; Banderas, Alvaro; Bec, Matthias Le et al. · 2020

Full title: The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments

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Full title: Pichia pastoris (Komagataella phaffii) as a Cost-Effective Tool for Vaccine Production for Low- and Middle-Income Countries (LMICs)

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Full title: Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi

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Full title: A Universal Strategy for the Efficient Expression of Nanobodies in Pichia pastoris

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Full title: Genetically Modified Micro-Organisms for Industrial Food Enzyme Production: An Overview

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Full title: Discovery and Heterologous Expression of Unspecific Peroxygenases

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Full title: High-Level Secretory Production of Recombinant E2-Spy Antigen Protein via Combined Strategy in Pichia pastoris

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Nikolaivits, Efstratios; Kanelli, Maria; Dimarogona, Maria et al. · 2018

Full title: A Middle-Aged Enzyme Still in Its Prime: Recent Advances in the Field of Cutinases

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Li, Tao; Ma, Jieying; Xu, Zehua et al. · 2019

Full title: Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3

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Capone, Simona; Ćorajević, Lejla; Bonifert, Günther et al. · 2015

Full title: Combining Protein and Strain Engineering for the Production of Glyco-Engineered Horseradish Peroxidase C1A in Pichia pastoris

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Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris

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Full title: Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris

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Ning, Xiaoyan; Zhang, Yanli; Yuan, Tiantian et al. · 2018

Full title: Enhanced Thermostability of Glucose Oxidase through Computer-Aided Molecular Design

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Full title: Efficient Heterologous Production of Rhizopus oryzae Lipase via Optimization of Multiple Expression-Related Helper Proteins.

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Full title: Production of a Recombinant Non-Hydroxylated Gelatin Mimetic in Pichia pastoris for Biomedical Applications

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Full title: A Thermostable Aspergillus fumigatus GH7 Endoglucanase Over-Expressed in Pichia pastoris Stimulates Lignocellulosic Biomass Hydrolysis

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Structural Homology Fails to Predict Secretion Efficiency in Pichia pastoris: Divergent Responses of Architecturally Similar scFvs to Multi-Parametric Genetic Engineering

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Full title: Structural Homology Fails to Predict Secretion Efficiency in Pichia pastoris: Divergent Responses of Architecturally Similar scFvs to Multi-Parametric Genetic Engineering

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Tullio, Viviana Cristina · 2022

Full title: Yeast Genomics and Its Applications in Biotechnological Processes: What Is Our Present and Near Future?

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High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris

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Full title: High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris

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Full title: Expression of Lectins in Heterologous Systems

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Applications of the Methylotrophic Yeast Komagataella phaffii in the Context of Modern Biotechnology

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Full title: Applications of the Methylotrophic Yeast Komagataella phaffii in the Context of Modern Biotechnology

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Full title: Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production

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DOI: https://doi.org/10.3390/metabo12040346

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Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins

Попова, Л. Г.; Khramov, Dmitrii E.; Nedelyaeva, Olga I. et al. · 2023

Full title: Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins

Authors: Попова, Л. Г.; Khramov, Dmitrii E.; Nedelyaeva, Olga I.; Volkov, Vadim

DOI: https://doi.org/10.3390/ijms241310768

Paper

Bioengineered Enzymes and Precision Fermentation in the Food Industry

Boukid, Fatma; Ganeshan, S.; Wang, Yingxin et al. · 2023

Full title: Bioengineered Enzymes and Precision Fermentation in the Food Industry

Authors: Boukid, Fatma; Ganeshan, S.; Wang, Yingxin; Tülbek, Mehmet; Nickerson, Michael T.

DOI: https://doi.org/10.3390/ijms241210156

Paper

Extracellular Microbial Metabolomics: The State of the Art

Pinu, Farhana R.; Villas‐Bôas, Silas G. · 2017

Full title: Extracellular Microbial Metabolomics: The State of the Art

Authors: Pinu, Farhana R.; Villas‐Bôas, Silas G.

DOI: https://doi.org/10.3390/metabo7030043

Paper

Comparison of Yeasts as Hosts for Recombinant Protein Production

Gomes, Antônio Milton Vieira; Carmo, Talita Souza; Carvalho, Lucas Silva et al. · 2018

Full title: Comparison of Yeasts as Hosts for Recombinant Protein Production

Authors: Gomes, Antônio Milton Vieira; Carmo, Talita Souza; Carvalho, Lucas Silva; Bahia, Frederico Mendonça; Parachin, Nádia Skorupa

DOI: https://doi.org/10.3390/microorganisms6020038

Paper

Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

J. Zha; Dan Liu; Juan Ren et al. · 2023

Full title: Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

Authors: J. Zha; Dan Liu; Juan Ren; Zhijun Liu; Xia Wu

DOI: https://doi.org/10.3390/jof9101027

Paper

Isolation and Characterization of the Diatom Phaeodactylum Δ5-Elongase Gene for Transgenic LC-PUFA Production in Pichia pastoris

Jiang, Mulan; Guo, Bing; Wan, Xia et al. · 2014

Full title: Isolation and Characterization of the Diatom Phaeodactylum Δ5-Elongase Gene for Transgenic LC-PUFA Production in Pichia pastoris

Authors: Jiang, Mulan; Guo, Bing; Wan, Xia; Gong, Yangmin; Zhang, Yinbo; Hu, Chuanjiong

DOI: https://doi.org/10.3390/md12031317

Paper

Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris

Jordà, Joel; Rojas, Hugo; Carnicer, Marc et al. · 2014

Full title: Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris

Authors: Jordà, Joel; Rojas, Hugo; Carnicer, Marc; Wahl, A.; Ferrer, Pau; Albiol, Joan

DOI: https://doi.org/10.3390/metabo4020281

Paper

Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms

Peebo, Karl; Neubauer, Peter · 2018

Full title: Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms

Authors: Peebo, Karl; Neubauer, Peter

DOI: https://doi.org/10.3390/microorganisms6030056

Paper

Laccase Gene Family in Cerrena sp. HYB07: Sequences, Heterologous Expression and Transcriptional Analysis

Yang, Jie; Xu, Xinqi; Ng, Tzi Bun et al. · 2016

Full title: Laccase Gene Family in Cerrena sp. HYB07: Sequences, Heterologous Expression and Transcriptional Analysis

Authors: Yang, Jie; Xu, Xinqi; Ng, Tzi Bun; Lin, Juan; Ye, Xiuyun

DOI: https://doi.org/10.3390/molecules21081017

Paper

Expression of Pleurotus ostreatus Laccase Gene in Pichia pastoris and Its Degradation of Corn Stover Lignin

Song, Qian; Deng, Xun; Song, Ruiqing · 2020

Full title: Expression of Pleurotus ostreatus Laccase Gene in Pichia pastoris and Its Degradation of Corn Stover Lignin

Authors: Song, Qian; Deng, Xun; Song, Ruiqing

DOI: https://doi.org/10.3390/microorganisms8040601

Paper

Evolving Paradigms of Recombinant Protein Production in Pharmaceutical Industry: A Rigorous Review

Jayakrishnan, Achuth; Rosli, Wan Rosalina Wan; Tahir, Ahmad Rashidi Mohamed et al. · 2024

Full title: Evolving Paradigms of Recombinant Protein Production in Pharmaceutical Industry: A Rigorous Review

DOI: https://doi.org/10.3390/sci6010009

Paper

Secretion and activity of antimicrobial peptide cecropin D expressed in Pichia pastoris

Guo, Chunhe; Huang, Yumao; Zheng, Hongyu et al. · 2012

Full title: Secretion and activity of antimicrobial peptide cecropin D expressed in Pichia pastoris

Authors: Guo, Chunhe; Huang, Yumao; Zheng, Hongyu; LiYun, Tang; He, Jun; LinSheng, Xiang; Liu, Dehui; JIANG, HOUQUAN

DOI: https://doi.org/10.3892/etm.2012.719

Paper

Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Komagataella phaffii Strains Utilizing Glycerol as Substrate

Melo, Nadielle Tamires Moreira; Pontes, Gabriela Coimbra; Procópio, Dielle Pierotti et al. · 2020

Full title: Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Komagataella phaffii Strains Utilizing Glycerol as Substrate

DOI: https://doi.org/10.3390/microorganisms8050781

Paper

Design, construction, and in vitro analysis of A33scFv::CDy, a recombinant fusion protein for antibody-directed enzyme prodrug therapy in colon cancer

Coelho, Vânia; Dernedde, Jens; Petrausch, Ulf et al. · 2007

Full title: Design, construction, and in vitro analysis of A33scFv::CDy, a recombinant fusion protein for antibody-directed enzyme prodrug therapy in colon cancer

Authors: Coelho, Vânia; Dernedde, Jens; Petrausch, Ulf; Panjideh, Hossein; Fuchs, Hendrik; Menzel, Christoph; Dübel, Stefan; Keilholz, Ulrich; Thiel, E.; Deckert, P. Markus

DOI: https://doi.org/10.3892/ijo.31.4.951

Paper

Expression and purification of FGF21 in Pichia pastoris and its effect on fibroblast-cell migration

Song, Yonghuan; Ding, Jian; Jin, Rilong et al. · 2016

Full title: Expression and purification of FGF21 in Pichia pastoris and its effect on fibroblast-cell migration

Authors: Song, Yonghuan; Ding, Jian; Jin, Rilong; Jung, Jin-Hee; Li, Shi; Yang, Jingquan; Wang, Anyuan; Li, Zhijie

DOI: https://doi.org/10.3892/mmr.2016.4942

Paper

Expression of porcine protegrin-1 in Pichia pastoris and its anticancer activity in vitro

Niu, Mingfu; Chai, Shumao; You, Xiaoyan et al. · 2015

Full title: Expression of porcine protegrin-1 in Pichia pastoris and its anticancer activity in vitro

Authors: Niu, Mingfu; Chai, Shumao; You, Xiaoyan; Wang, Wenhui; Qin, Cuili; Gong, Qiang; Zhang, Tingting; Wan, Peng

DOI: https://doi.org/10.3892/etm.2015.2202

Paper

Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin-2 in Pichia pastoris

Xing, Li-Wei; Tian, Shi-Xun; Gao, Wei et al. · 2016

Full title: Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin-2 in Pichia pastoris

Authors: Xing, Li-Wei; Tian, Shi-Xun; Gao, Wei; Yang, Na; Qu, Pei; Liu, Di; Jiao, Jian; Wang, Jue; Feng, Xingjun

DOI: https://doi.org/10.3892/etm.2016.3578

Paper

Cholera Toxin Subunit B as Adjuvant––An Accelerator in Protective Immunity and a Break in Autoimmunity

Stratmann, Thomas · 2015

Full title: Cholera Toxin Subunit B as Adjuvant––An Accelerator in Protective Immunity and a Break in Autoimmunity

Authors: Stratmann, Thomas

DOI: https://doi.org/10.3390/vaccines3030579

Paper

Secretion and expression dynamics of a GFP-tagged mucin-type fusion protein in high cell density Pichia pastoris bioreactor cultivations

M. Sjöblom; L. Lindberg; J. Holgersson et al. · 2012

Full title: Secretion and expression dynamics of a GFP-tagged mucin-type fusion protein in high cell density Pichia pastoris bioreactor cultivations

Authors: M. Sjöblom; L. Lindberg; J. Holgersson; U. Rova

DOI: https://doi.org/10.4236/abb.2012.33033

Paper

Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Durmusoglu, Deniz; Al’Abri, Ibrahim S.; Li, Zidan et al. · 2023

Full title: Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Authors: Durmusoglu, Deniz; Al’Abri, Ibrahim S.; Li, Zidan; Williams, Taufika Islam; Collins, Leonard B.; Martínez, José L.; Crook, Nathan

DOI: https://doi.org/10.1186/s12934-023-02117-y

Paper

Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability

Kangwa, Martin; Salgado, José Antonio Gama; Fernández‐Lahore, Marcelo · 2018

Full title: Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability

Authors: Kangwa, Martin; Salgado, José Antonio Gama; Fernández‐Lahore, Marcelo

DOI: https://doi.org/10.1186/s13568-018-0691-3

Paper

Recent Advances in the Metabolic Engineering of Yeasts for Ginsenoside Biosynthesis

Chu, Luan Luong; Montecillo, Jake Adolf V.; Bae, Hanhong · 2020

Full title: Recent Advances in the Metabolic Engineering of Yeasts for Ginsenoside Biosynthesis

Authors: Chu, Luan Luong; Montecillo, Jake Adolf V.; Bae, Hanhong

DOI: https://doi.org/10.3389/fbioe.2020.00139

Thesis

Expression of human epidermal growth factor in pichia pastoris and optimization of fermentation conditions

Eissazadeh, Samira · 2012

Full title: Expression of human epidermal growth factor in pichia pastoris and optimization of fermentation conditions

Authors: Eissazadeh, Samira

DOI: http://psasir.upm.edu.my/id/eprint/31605/1/FBSB%202012%2030R.pdf

Thesis

Eplt4 Proteinaceous Elicitor Produced in Pichia pastoris Has a Protective Effect Against Cercosporidium sofinum Infections of Soybean Leaves

Wang, Y; Song, J; Wu, Y et al. · 2021

Full title: Eplt4 Proteinaceous Elicitor Produced in Pichia pastoris Has a Protective Effect Against Cercosporidium sofinum Infections of Soybean Leaves

Authors: Wang, Y; Song, J; Wu, Y; Odeph, M; Liu, Z; Howlett, BJ; Wang, S; Yang, P; Yao, L; Zhao, L; Yang, Q

DOI: https://hdl.handle.net/11343/283084

Thesis

Development of yeast expression systems for producing thermostable T1 lipase

Oslan, Siti Nurbaya · 2012

Full title: Development of yeast expression systems for producing thermostable T1 lipase

Authors: Oslan, Siti Nurbaya

DOI: http://psasir.upm.edu.my/id/eprint/33480/1/FBSB%202012%2026R.pdf

Thesis

Expression of recombinant thermostable W200R protease in Pichia pastoris

Mohamad Sultan, Muhamad Zarir · 2011

Full title: Expression of recombinant thermostable W200R protease in Pichia pastoris

Authors: Mohamad Sultan, Muhamad Zarir

DOI: http://psasir.upm.edu.my/id/eprint/67162/1/IB%202012%2028%20IR.pdf

Thesis

Development of protease deficient Meyerozyma guilliermondii strain so for overexpression of thermostable T1 lipase

Lorrine, Okojie Eseoghene · 2022

Full title: Development of protease deficient Meyerozyma guilliermondii strain so for overexpression of thermostable T1 lipase

Authors: Lorrine, Okojie Eseoghene

DOI: http://psasir.upm.edu.my/id/eprint/104584/1/FBSB%202022%2010%20IR.pdf

Thesis

Molecular characterization and expression of H5, N1 and NS1 recombinant proteins of avian influenza virus subtype H5N1 in Pichia pastoris

Abubakar, Mustapha Bala · 2011

Full title: Molecular characterization and expression of H5, N1 and NS1 recombinant proteins of avian influenza virus subtype H5N1 in Pichia pastoris

Authors: Abubakar, Mustapha Bala

DOI: http://psasir.upm.edu.my/id/eprint/70100/1/FPV%202011%2019%20-%20IR.pdf

Thesis

Production of thermostable L2 lipase using recombinant Pichia pastoris

Shahidan, Noor Hidayah · 2012

Full title: Production of thermostable L2 lipase using recombinant Pichia pastoris

Authors: Shahidan, Noor Hidayah

DOI: http://psasir.upm.edu.my/id/eprint/32372/1/IB%202012%207R%20pdf.pdf

Thesis

Expression And Characterization Of Recombinant Thermostable L2 Lipase In Pichia Pastoris

Sabri, Suriana · 2007

Full title: Expression And Characterization Of Recombinant Thermostable L2 Lipase In Pichia Pastoris

Authors: Sabri, Suriana

DOI: http://psasir.upm.edu.my/id/eprint/4857/1/FBSB_2007_1.pdf

Thesis

Enhancing recombinant T1 lipase production in Pichia guilliermondii

Ladidi, Abu Mary · 2017

Full title: Enhancing recombinant T1 lipase production in Pichia guilliermondii

Authors: Ladidi, Abu Mary

DOI: http://psasir.upm.edu.my/id/eprint/70201/1/FBSB%202017%2019%20-%20IR.pdf

Thesis

Establishment of optimise strategies in production of recombinant Hepatitis B surface antigen for chip-based antibody detection approach

Tam, Yew Joon · 2015

Full title: Establishment of optimise strategies in production of recombinant Hepatitis B surface antigen for chip-based antibody detection approach

Authors: Tam, Yew Joon

DOI: http://psasir.upm.edu.my/id/eprint/64029/1/IB%202015%2011.pdf

Thesis

Expression of Alkaline Protease from Bacillus Stearothermophilus F1 in Pichia Pastoris

Ahmad Latiffi, Amaliawati · 2008

Full title: Expression of Alkaline Protease from Bacillus Stearothermophilus F1 in Pichia Pastoris

Authors: Ahmad Latiffi, Amaliawati

DOI: http://psasir.upm.edu.my/id/eprint/5394/1/IB_2008_6A.pdf

Thesis

Structural investigation of alcohol oxidase from Meyerozyma guilliermondii and the use of its promoter for recombinant protein expression

Mahyon, Nur Iznida · 2017

Full title: Structural investigation of alcohol oxidase from Meyerozyma guilliermondii and the use of its promoter for recombinant protein expression

Authors: Mahyon, Nur Iznida

DOI: http://psasir.upm.edu.my/id/eprint/84634/1/FBSB%202019%2017%20-%20ir.pdf

Thesis

Expression of recombinant geobacillus SP a-amylase in pichia pastoris GS 115

Gandhi, Sivasangkary · 2014

Full title: Expression of recombinant geobacillus SP a-amylase in pichia pastoris GS 115

Authors: Gandhi, Sivasangkary

DOI: http://psasir.upm.edu.my/id/eprint/56821/1/IB%202014%2018.pdf

Thesis

HSF-1, HIF-1and HSP90 expression on recombinant pichia pastoris under fed-batch fermentation

Farías, Jorge G · 2014

Full title: HSF-1, HIF-1and HSP90 expression on recombinant pichia pastoris under fed-batch fermentation

Authors: Farías, Jorge G

DOI: http://www.scielo.br/pdf/bjm/v45n2/15.pdf

Thesis

Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris

Farías, Jorge G · 2014

Full title: Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris

Authors: Farías, Jorge G

DOI: http://www.scielo.br/pdf/bjm/v45n2/14.pdf

Thesis

Applications of recombinant Pichia pastoris in the healthcare industry

Farías, Jorge G · 2013

Full title: Applications of recombinant Pichia pastoris in the healthcare industry

Authors: Farías, Jorge G

DOI: http://www.scielo.br/pdf/bjm/v44n4/04.pdf

Thesis

Fed-Batch Production of Saccharomyces cerevisiae L-Asparaginase II by Recombinant Pichia pastoris MUTs Strain

Pessoa Junior, Adalberto · 2019

Full title: Fed-Batch Production of Saccharomyces cerevisiae L-Asparaginase II by Recombinant Pichia pastoris MUTs Strain

Authors: Pessoa Junior, Adalberto

DOI: https://doi.org/10.3389/fbioe.2019.00016

Thesis

Advances in the regulation and production of fungal enzymes by transcriptomics, proteomics and recombinant strains design. [Editorial]

Segato, Fernando · 2019

Full title: Advances in the regulation and production of fungal enzymes by transcriptomics, proteomics and recombinant strains design. [Editorial]

Authors: Segato, Fernando

DOI: https://doi.org/10.3389/fbioe.2019.00157

Thesis

Systems and synthetic biology approaches to engineer fungi for fine chemical production

Silva-Rocha, Rafael · 2018

Full title: Systems and synthetic biology approaches to engineer fungi for fine chemical production

Authors: Silva-Rocha, Rafael

DOI: https://doi.org/10.3389/fbioe.2018.00117

Thesis

Yeasts as biopharmaceutical production platforms

Courdavault, Vincent · 2021

Full title: Yeasts as biopharmaceutical production platforms

Authors: Courdavault, Vincent

DOI: https://doi.org/10.3389/ffunb.2021.733492

Thesis

Expression and Mutagenesis studies of Candida antactica lipase B

Rotticci-Mulder, Johanna C. · 2003

Full title: Expression and Mutagenesis studies of Candida antactica lipase B

Authors: Rotticci-Mulder, Johanna C.

DOI: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3493

Thesis

Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris.

Canales, M.; Perez de la Lastra, J.M.; Naranjo, V. et al. · 2009

Full title: Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris.

Authors: Canales, M.; Perez de la Lastra, J.M.; Naranjo, V.; Nijhof, A.M.; Hope, M.; Jongejan, F.; de la Fuente, J.

DOI: https://dspace.library.uu.nl/handle/1874/37475

Thesis

High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG

Blanchard, Véronique; Gadkari, R.P; George, A.V.E. et al. · 2008

Authors: Blanchard, Véronique; Gadkari, R.P; George, A.V.E.; Roy, R.; Gerwig, G.J.; Leeflang, B.R.; Dighe, R.R.; Boelens, R.; Kamerling, J.P.

DOI: https://dspace.library.uu.nl/handle/1874/29965

Thesis

Comparative genomics and transcriptomics of Pichia pastoris

Priest, Margaret; Borowsky, Jonathan; Young, Sarah K. et al. · 2017

Full title: Comparative genomics and transcriptomics of Pichia pastoris

Authors: Priest, Margaret; Borowsky, Jonathan; Young, Sarah K.; Love, Kerry R.; Love, John C; Wu, Jie; Ma, Duanduan; Shah, Kartik A; Bartlett, Mary Catherine; Leeson, Rachel; Whittaker, Charles A.

DOI: http://hdl.handle.net/1721.1/107984

Thesis

A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

Lee, Kevin S.; Lee, Harry L. T.; Mozdzierz, Nicholas Joseph et al. · 2017

Full title: A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

Authors: Lee, Kevin S.; Lee, Harry L. T.; Mozdzierz, Nicholas Joseph; Love, Kerry R.; Shah, Kartik A; Ram, Rajeev J; Love, John C

DOI: http://hdl.handle.net/1721.1/109453

Thesis

Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris

Shah, Kartik A; Clark, John J; Goods, Brittany A. et al. · 2017

Full title: Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris

Authors: Shah, Kartik A; Clark, John J; Goods, Brittany A.; Politano, Timothy James; Mozdzierz, Nicholas Joseph; Zimnisky, Ross; Leeson, Rachel; Love, John C; Love, Kerry R.

DOI: http://hdl.handle.net/1721.1/109438

Thesis

Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care

Cleto, Sara; Lee, Kevin; Perez-Pinera, Pablo et al. · 2017

Full title: Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care

Authors: Cleto, Sara; Lee, Kevin; Perez-Pinera, Pablo; Han, Ningren; Cao, Jicong; Purcell, Oliver; Shah, Kartik A; Ram, Rajeev J; Lu, Timothy K

DOI: http://hdl.handle.net/1721.1/109076

Thesis

Eukaryotic expression: developments for structural proteomics

Aricescu, A. R.; Assenberg, R.; Bill, R. M. et al. · 2006

Full title: Eukaryotic expression: developments for structural proteomics

DOI: https://dspace.library.uu.nl/handle/1874/357756

Thesis

Establishment of a novel Pichia Pastoris host production platform

Coleman, Ellen(Ellen M.) · 2020

Full title: Establishment of a novel Pichia Pastoris host production platform

Authors: Coleman, Ellen(Ellen M.)

DOI: https://hdl.handle.net/1721.1/126948

Thesis

Development of a platform process for the production and purification of single‐domain antibodies

Crowell, Laura E; Goodwine, Chaz; Holt, Carla S et al. · 2021

Full title: Development of a platform process for the production and purification of single‐domain antibodies

DOI: https://hdl.handle.net/1721.1/135524

Thesis

Macroscopic modeling of bioreactors for recombinant protein producing Pichia pastoris in defined medium

Hong, Moo Sun; Velez-Suberbie, M Lourdes; Maloney, Andrew J et al. · 2021

Full title: Macroscopic modeling of bioreactors for recombinant protein producing Pichia pastoris in defined medium

Authors: Hong, Moo Sun; Velez-Suberbie, M Lourdes; Maloney, Andrew J; Biedermann, Andrew; Love, Kerry R; Love, J Christopher; Mukhopadhyay, Tarit K; Braatz, Richard D

DOI: https://hdl.handle.net/1721.1/133351

Thesis

Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain

Brady, Joseph R; Whittaker, Charles A; Tan, Melody C et al. · 2021

Full title: Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain

Authors: Brady, Joseph R; Whittaker, Charles A; Tan, Melody C; Kristensen, D Lee; Ma, Duanduan; Dalvie, Neil C; Love, Kerry Routenberg; Love, J Christopher

DOI: https://hdl.handle.net/1721.1/136502

Thesis

CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome

Dalvie, Neil C.; Lorgeree, Timothy R.; Yang, Yuchen et al. · 2024

Full title: CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome

DOI: https://hdl.handle.net/1721.1/155951

Thesis

Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii

Dalvie, Neil C.; Leal, Justin; Whittaker, Charles A. et al. · 2022

Full title: Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii

Authors: Dalvie, Neil C.; Leal, Justin; Whittaker, Charles A.; Yang, Yuchen; Brady, Joseph R.; Love, Kerry R.; Love, J. Christopher

DOI: https://hdl.handle.net/1721.1/136627.2

Thesis

Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

Dalvie, Neil C.; Naranjo, Christopher A.; Rodriguez-Aponte, Sergio A. et al. · 2022

Full title: Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella phaffii

Authors: Dalvie, Neil C.; Naranjo, Christopher A.; Rodriguez-Aponte, Sergio A.; Johnston, Ryan S.; Christopher Love, J.

DOI: https://hdl.handle.net/1721.1/145353

Thesis

Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

Dalvie, Neil C; Brady, Joseph R; Crowell, Laura E et al. · 2021

Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

DOI: https://hdl.handle.net/1721.1/136780

Thesis

Enhanced Expression of Alcohol Dehydrogenase I in Pichia pastoris Reduces the Content of Acetaldehyde in Wines

Geng, Kun; Lin, Ying; Zheng, Xueyun et al. · 2024

Full title: Enhanced Expression of Alcohol Dehydrogenase I in Pichia pastoris Reduces the Content of Acetaldehyde in Wines

Authors: Geng, Kun; Lin, Ying; Zheng, Xueyun; Li, Cheng; Chen, Shuting; Ling, He; Yang, Jun; Zhu, Xiangyu; Liang, Shuli

DOI: https://hdl.handle.net/1721.1/153421

Thesis

Adaptation of Aglycosylated Monoclonal Antibodies for Improved Production in Komagataella phaffii

Yang, Yuchen; Dalvie, Neil C; Brady, Joseph R et al. · 2025

Full title: Adaptation of Aglycosylated Monoclonal Antibodies for Improved Production in Komagataella phaffii

DOI: https://hdl.handle.net/1721.1/163225

Thesis

Systematic Single-Cell Analysis of Pichia pastoris Reveals Secretory Capacity Limits Productivity

Politano, Timothy James; Panagiotou, Vasiliki; Stadheim, Terrance A. et al. · 2012

Full title: Systematic Single-Cell Analysis of Pichia pastoris Reveals Secretory Capacity Limits Productivity

Authors: Politano, Timothy James; Panagiotou, Vasiliki; Stadheim, Terrance A.; Love, Kerry R.; Jiang, Bo, S.M. Massachusetts Institute of Technology; Love, John C

DOI: http://hdl.handle.net/1721.1/72414

Thesis

A central composite design to investigate antibody fragment production by Pichia pastoris

Chan, Joyce, M. Eng. Massachusetts Institute of Technology · 2006

Full title: A central composite design to investigate antibody fragment production by Pichia pastoris

Authors: Chan, Joyce, M. Eng. Massachusetts Institute of Technology

DOI: http://hdl.handle.net/1721.1/33872

Thesis

Clonal selection and characterization of epigenetic variation in Pichia pastoris

Panagiotou, Vasiliki · 2010

Full title: Clonal selection and characterization of epigenetic variation in Pichia pastoris

Authors: Panagiotou, Vasiliki

DOI: http://hdl.handle.net/1721.1/59881

Thesis

Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.

Love, Kerry Routenberg; Panagiotou, Vasiliki; Jiang, Bo et al. · 2012

Full title: Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.

Authors: Love, Kerry Routenberg; Panagiotou, Vasiliki; Jiang, Bo; Stadheim, Terrance A.; Love, J. Christopher

DOI: http://hdl.handle.net/1721.1/69920

Thesis

Targeted detection of secretion pathway protein interactions for therapeutic production and cellular engineering

Robinson, Caressa Mystique · 2020

Full title: Targeted detection of secretion pathway protein interactions for therapeutic production and cellular engineering

Authors: Robinson, Caressa Mystique

DOI: https://escholarship.org/uc/item/4q15r9h9

Thesis

A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable

Leynaud-Kieffer, Laure M. C.; Curran, Samuel C.; Kim, Irene et al. · 2019

Full title: A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable

Authors: Leynaud-Kieffer, Laure M. C.; Curran, Samuel C.; Kim, Irene; Magnuson, Jon K.; Gladden, John M.; Baker, Scott E.; Simmons, Blake A.

DOI: https://infoscience.epfl.ch/handle/20.500.14299/157981

Thesis

Produção biotecnológica de L-asparaginase (ASP1) de Saccharomyces cerevisiae em sistema de expressão heterólogo Pichia pastoris

Monteiro, Gisele · 2015

Full title: Produção biotecnológica de L-asparaginase (ASP1) de Saccharomyces cerevisiae em sistema de expressão heterólogo Pichia pastoris

Authors: Monteiro, Gisele

DOI: http://www.teses.usp.br/teses/disponiveis/9/9134/tde-15122015-172127/

Thesis

Expressão de fragmentos variáveis de cadeia simples anti-LDL eletronegativa (scFv) em Pichia pastoris e seu efeito sobre a formação de células espumosas

Abdalla, Dulcinéia Saes Parra · 2010

Full title: Expressão de fragmentos variáveis de cadeia simples anti-LDL eletronegativa (scFv) em Pichia pastoris e seu efeito sobre a formação de células espumosas

Authors: Abdalla, Dulcinéia Saes Parra

DOI: http://www.teses.usp.br/teses/disponiveis/9/9136/tde-08072010-141439/

Thesis

Clonagem e expressão heteróloga do gene de uma xilanase de Thermoascus aurantiacus em Pichia pastoris

Franco, Fernanda Craveiro [UNESP] · 2014

Full title: Clonagem e expressão heteróloga do gene de uma xilanase de Thermoascus aurantiacus em Pichia pastoris

Authors: Franco, Fernanda Craveiro [UNESP]

DOI: http://hdl.handle.net/11449/94847

Thesis

An improved process for the production of highly purified recombinant thaumatin tagged-variants

Healey, RD; Lebhar, H; https://orcid.org/0000-0002-9023-2467 et al. · 2017

Full title: An improved process for the production of highly purified recombinant thaumatin tagged-variants

Authors: Healey, RD; Lebhar, H ; https://orcid.org/0000-0002-9023-2467; Hornung, S; Thordarson, P ; https://orcid.org/0000-0002-1200-8814; Marquis, CP ; https://orcid.org/0000-0003-1656-7215

DOI: http://hdl.handle.net/1959.4/unsworks_73890

Thesis

Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris.

Gurkan, Cemal; Ellar, David J · 2011

Full title: Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris.

Authors: Gurkan, Cemal; Ellar, David J

DOI: http://www.dspace.cam.ac.uk/handle/1810/238082

Thesis

Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris.

Whyteside, Graham; Alcocer, Marcos JC; Kumita, Janet R et al. · 2020

Full title: Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris.

Authors: Whyteside, Graham; Alcocer, Marcos JC; Kumita, Janet R; Dobson, Christopher M; Lazarou, Maria; Pleass, Richard J; Archer, David B

DOI: https://www.repository.cam.ac.uk/handle/1810/309386

Thesis

LPS ligand and culture additives improve production of monomeric MD-1 and 2 in Pichia pastoris by decreasing aggregation and intermolecular disulfide bonding.

Mengwasser, Kristen E; Bryant, Clare E; Gay, Nick J et al. · 2016

Full title: LPS ligand and culture additives improve production of monomeric MD-1 and 2 in Pichia pastoris by decreasing aggregation and intermolecular disulfide bonding.

Authors: Mengwasser, Kristen E; Bryant, Clare E; Gay, Nick J; Gangloff, Monique

DOI: https://www.repository.cam.ac.uk/handle/1810/260369

Thesis

Produção de fragmento recombinante de anticorpo em Pichia pastoris

Pessoa Junior, Adalberto · 2014

Full title: Produção de fragmento recombinante de anticorpo em Pichia pastoris

Authors: Pessoa Junior, Adalberto

DOI: http://www.teses.usp.br/teses/disponiveis/9/9134/tde-10062014-084638/

Thesis

Produção do fragmento de anticorpo scFv por Pichia pastoris geneticamente modificada

Pessoa Junior, Adalberto · 2013

Full title: Produção do fragmento de anticorpo scFv por Pichia pastoris geneticamente modificada

Authors: Pessoa Junior, Adalberto

DOI: http://www.teses.usp.br/teses/disponiveis/9/9135/tde-28052015-103520/

Thesis

Produção biotecnológica de L-asparaginase(ASP3) de Saccharomyces cerevisiae em sistema de expressão heterólogo Pichia pastoris

Monteiro, Gisele · 2015

Full title: Produção biotecnológica de L-asparaginase(ASP3) de Saccharomyces cerevisiae em sistema de expressão heterólogo Pichia pastoris

Authors: Monteiro, Gisele

DOI: http://www.teses.usp.br/teses/disponiveis/9/9134/tde-15122015-082825/

Thesis

Humanização específica do sistema de glicosilação de Pichia pastoris pela técnica CRISPR/Cas9 visando a expressão de glicoproteínas humanas

Ho, Paulo Lee · 2016

Full title: Humanização específica do sistema de glicosilação de Pichia pastoris pela técnica CRISPR/Cas9 visando a expressão de glicoproteínas humanas

Authors: Ho, Paulo Lee

DOI: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-11042017-084657/

Thesis

Expressão da L-asparaginase II de Saccharomyces cerevisiae recombinante extracelular com glicosilação humanizada em Pichia pastoris

Monteiro, Gisele · 2019

Full title: Expressão da L-asparaginase II de Saccharomyces cerevisiae recombinante extracelular com glicosilação humanizada em Pichia pastoris

Authors: Monteiro, Gisele

DOI: http://www.teses.usp.br/teses/disponiveis/9/9134/tde-02102019-150712/

Thesis

Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso

Russo, Elisa Maria de Sousa · 2017

Full title: Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso

Authors: Russo, Elisa Maria de Sousa

DOI: https://www.teses.usp.br/teses/disponiveis/60/60135/tde-23112017-101039/

Thesis

Transcriptoma e expressão recombinante de peptidases do fungo Phanerochaete chrysosporium em Pichia pastoris

Cabral, Hamilton · 2019

Full title: Transcriptoma e expressão recombinante de peptidases do fungo Phanerochaete chrysosporium em Pichia pastoris

Authors: Cabral, Hamilton

DOI: http://www.teses.usp.br/teses/disponiveis/60/60137/tde-18122019-171832/

Thesis

Expanding the metabolic engineering toolbox to rationally and predictably modulate gene expression at the transcriptional and translational level

Peters, Gert · 2017

Full title: Expanding the metabolic engineering toolbox to rationally and predictably modulate gene expression at the transcriptional and translational level

Authors: Peters, Gert

DOI: https://biblio.ugent.be/publication/8518664

Thesis

Development of molecular tools for Komagataella phaffii and exploring its use for orally administered biopharmaceuticals

Vanluchene, Robin · 2020

Full title: Development of molecular tools for Komagataella phaffii and exploring its use for orally administered biopharmaceuticals

Authors: Vanluchene, Robin

DOI: https://biblio.ugent.be/publication/8684279

Thesis

Design of a cultivation medium for protein production in Pichia pastoris based on genome-wide biological understanding

Matthews, Catherine Bartlett. · 2019

Full title: Design of a cultivation medium for protein production in Pichia pastoris based on genome-wide biological understanding

Authors: Matthews, Catherine Bartlett.

DOI: https://hdl.handle.net/1721.1/122528

Thesis

Product and host engineering for low-cost manufacturing of therapeutic proteins in the yeast Komagataella phaffii

Dalvie, Neil Chandra · 2022

Full title: Product and host engineering for low-cost manufacturing of therapeutic proteins in the yeast Komagataella phaffii

Authors: Dalvie, Neil Chandra

DOI: https://hdl.handle.net/1721.1/144884

Thesis

An integrated approach to enable rapid scalable upstream production of subunit vaccines with Pichia pastoris (Komagataella phaffii)

Biedermann, Andrew M. · 2022

Full title: An integrated approach to enable rapid scalable upstream production of subunit vaccines with Pichia pastoris (Komagataella phaffii)

Authors: Biedermann, Andrew M.

DOI: https://hdl.handle.net/1721.1/144883

Thesis

Molecular, Genetic, and Process Approaches for Improving Secreted Pharmaceutical Protein Quality in Komagataella phaffii

Yang, Yuchen · 2024

Full title: Molecular, Genetic, and Process Approaches for Improving Secreted Pharmaceutical Protein Quality in Komagataella phaffii

Authors: Yang, Yuchen

DOI: https://hdl.handle.net/1721.1/157251

Thesis

Kinetics and modeling of protein production in a recombinant yeast containing temperature-regulated promoters /

Shields, Richard William · 1989

Full title: Kinetics and modeling of protein production in a recombinant yeast containing temperature-regulated promoters /

Authors: Shields, Richard William

DOI: http://rave.ohiolink.edu/etdc/view?acc_num=osu1749745350688424

Thesis

GENERATION, CLONING, AND SMALL-SCALE EXPRESSION OF SITE-DIRECTED MUTANTS OF HEN EGG WHITE LYSOZYME IN PICHIA PASTORIS

Patton, Nichole L. · 2012

Full title: GENERATION, CLONING, AND SMALL-SCALE EXPRESSION OF SITE-DIRECTED MUTANTS OF HEN EGG WHITE LYSOZYME IN PICHIA PASTORIS

Authors: Patton, Nichole L.

DOI: http://rave.ohiolink.edu/etdc/view?acc_num=ysu1348846216

Thesis

Exploring the dual roles of tRNAs in Komagataella phaffii production hosts

Chahid, Nisreen · 2024

Full title: Exploring the dual roles of tRNAs in Komagataella phaffii production hosts

Authors: Chahid, Nisreen

DOI: https://etheses.whiterose.ac.uk/id/eprint/37710/1/Chahid_203013793_CorrectedThesisClean.pdf

Thesis

An optimized high throughput platform, from genetic transformation to fermentation, to screen for secreted laccases in Aspergillus niger

Leynaud-Kieffer Curran, Laure Marie Colette · 2022

Full title: An optimized high throughput platform, from genetic transformation to fermentation, to screen for secreted laccases in Aspergillus niger

Authors: Leynaud-Kieffer Curran, Laure Marie Colette

DOI: https://infoscience.epfl.ch/handle/20.500.14299/184440

Thesis

Quantitative characterization of a recombinant Pichia pastoris Mut+ strain secreting avidin using transient continuous cultures

Jungo, Carmen · 2007

Full title: Quantitative characterization of a recombinant Pichia pastoris Mut+ strain secreting avidin using transient continuous cultures

Authors: Jungo, Carmen

DOI: https://infoscience.epfl.ch/handle/20.500.14299/6151

Thesis

Bench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris based expression systems

Ramchuran, Santosh · 2005

Full title: Bench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris based expression systems

Authors: Ramchuran, Santosh

DOI: https://lup.lub.lu.se/record/544473

Thesis

Pichia pastoris as Enzyme Production Host: from Toolkit Design to Protein Expression

Obst, Ulrike · 2019

Full title: Pichia pastoris as Enzyme Production Host: from Toolkit Design to Protein Expression

Authors: Obst, Ulrike

DOI: https://mediatum.ub.tum.de/1462686

Thesis

Enhancing Eukaryotic Cell-Free Systems through Genetic Engineering and Optimization of Protein Production Processes

Schloßhauer, Jeffrey Lesslie · 2024

Full title: Enhancing Eukaryotic Cell-Free Systems through Genetic Engineering and Optimization of Protein Production Processes

Authors: Schloßhauer, Jeffrey Lesslie

DOI: https://refubium.fu-berlin.de/handle/fub188/45613

Thesis

Design, expression, and testing of a bivalent subunit botulism vaccine for use in cattle

Shtevi, Abraham · 2009

Full title: Design, expression, and testing of a bivalent subunit botulism vaccine for use in cattle

Authors: Shtevi, Abraham

DOI: https://researchonline.jcu.edu.au/11603/13/JCU_11603_Shtevi_2009_thesis.pdf

Thesis

Optimization of the production of recombinant macrophage inhibitory cytokine 1 (MIC-1) from Pichia pastoris

Pratanaphon, Ronachai · 2006

Full title: Optimization of the production of recombinant macrophage inhibitory cytokine 1 (MIC-1) from Pichia pastoris

Authors: Pratanaphon, Ronachai

DOI: http://hdl.handle.net/1959.4/68401

Thesis

Improving protein yield from mammalian cells by manipulation of stress response pathways

Chalmers, Fiona · 2016

Full title: Improving protein yield from mammalian cells by manipulation of stress response pathways

Authors: Chalmers, Fiona

DOI: https://theses.gla.ac.uk/7666/7/2016ChalmersPhD.pdf

Thesis

Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production

Zhang, Wei · 2014

Full title: Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production

Authors: Zhang, Wei

DOI: http://hdl.handle.net/10919/33395

Thesis

Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter

Barrigón de San Marcos, José Manuel · 2015

Full title: Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter

Authors: Barrigón de San Marcos, José Manuel

DOI: http://hdl.handle.net/10803/287984

Thesis

Lipidomics studies of recombinant Pichia pastoris for improved recombinant protein secretion through cell engineering

Adelantado Vallvé, Núria · 2016

Full title: Lipidomics studies of recombinant Pichia pastoris for improved recombinant protein secretion through cell engineering

Authors: Adelantado Vallvé, Núria

DOI: http://hdl.handle.net/10803/384229

Thesis

Development of Pichia pastoris for the production of human α-galactosidase A as a model lysosomal enzyme

Barreiro Vázquez, Antonio · 2016

Full title: Development of Pichia pastoris for the production of human α-galactosidase A as a model lysosomal enzyme

Authors: Barreiro Vázquez, Antonio

DOI: http://hdl.handle.net/10803/382822

Thesis

A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches

Garcia Ortega, Xavier · 2016

Full title: A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches

Authors: Garcia Ortega, Xavier

DOI: http://hdl.handle.net/10803/384531

Thesis

Anàlisi quantitatiu de l’efecte de l’expressió d’una lipasa recombinant sobre el metabolisme central de Pichia pastoris

Jordà Murria, Joel · 2013

Full title: Anàlisi quantitatiu de l’efecte de l’expressió d’una lipasa recombinant sobre el metabolisme central de Pichia pastoris

Authors: Jordà Murria, Joel

DOI: http://hdl.handle.net/10803/117310

Thesis

L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic

Cámara Rey, Elena · 2017

Authors: Cámara Rey, Elena

DOI: http://hdl.handle.net/10803/399833

Thesis

Bioprocess efficiency as a roadmap to establish Pichia pastoris as a reliable cell factory

Ponte Font, Xavier · 2017

Full title: Bioprocess efficiency as a roadmap to establish Pichia pastoris as a reliable cell factory

Authors: Ponte Font, Xavier

DOI: http://hdl.handle.net/10803/457712

Thesis

Development of the production process for a recombinant hormone and evaluation of its biological activity

Font Ingles, Albert · 2013

Full title: Development of the production process for a recombinant hormone and evaluation of its biological activity

Authors: Font Ingles, Albert

DOI: http://hdl.handle.net/10803/123298

Thesis

Developing strategies for systems metabolic engineering of Pichia pastoris

Tomàs Gamisans, Màrius · 2017

Full title: Developing strategies for systems metabolic engineering of Pichia pastoris

Authors: Tomàs Gamisans, Màrius

DOI: http://hdl.handle.net/10803/458538

Thesis

Overcoming the secretory limitations in Pichia pastoris for recombinant protein production

Barrero Peña, Juan José · 2021

Full title: Overcoming the secretory limitations in Pichia pastoris for recombinant protein production

Authors: Barrero Peña, Juan José

DOI: http://hdl.handle.net/10803/670387

Thesis

Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris

Garrigós Martínez, Javier · 2021

Full title: Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris

Authors: Garrigós Martínez, Javier

DOI: http://hdl.handle.net/10803/671119

Thesis

Estratègies d’operació per a la producció de lipases de Rhizopus oryzae amb el fenotip Mut(s) de Pichia pastoris mitjançant substrats mixtes

Arnau Jiménez, Carol · 2011

Full title: Estratègies d’operació per a la producció de lipases de Rhizopus oryzae amb el fenotip Mut(s) de Pichia pastoris mitjançant substrats mixtes

Authors: Arnau Jiménez, Carol

DOI: http://hdl.handle.net/10803/42007

Thesis

Systematic metabolic analysis of recombinant Pichia pastoris under different oxygen conditions A Metabolome and Fluxome Based Study

Carnicer Heras, Marc · 2012

Full title: Systematic metabolic analysis of recombinant Pichia pastoris under different oxygen conditions A Metabolome and Fluxome Based Study

Authors: Carnicer Heras, Marc

DOI: http://hdl.handle.net/10803/96813

Thesis

Systems metabolic engineering for recombinant protein production in Pichia pastoris

Monforte Mercado, Sergi · 2020

Full title: Systems metabolic engineering for recombinant protein production in Pichia pastoris

Authors: Monforte Mercado, Sergi

DOI: http://hdl.handle.net/10803/669385

Thesis

Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris

Nieto Taype, Miguel Angel · 2020

Full title: Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris

Authors: Nieto Taype, Miguel Angel

DOI: http://hdl.handle.net/10803/670163

Thesis

Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess

Gasset Franch, Arnau · 2024

Full title: Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess

Authors: Gasset Franch, Arnau

DOI: http://hdl.handle.net/10803/691293

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· 1992

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· 2017

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Strain Engineering For Pichia Pastoris Protein Sec

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