PaperImpact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-state-maintained continuous cultures
Korpys-Woźniak, Paulina, Kubiak, Piotr, Białas, W., Celińska, Ewelina · 2020 · Applied Microbiology and Biotechnology · DOI
Full title: Impact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-state-maintained continuous cultures
Authors: Korpys-Woźniak, Paulina, Kubiak, Piotr, Białas, W., Celińska, Ewelina
Full title: Heterologous expression and optimization of the antimicrobial peptide acidocin 4356 in Komagataella phaffii to target Pseudomonas aeruginosa.
PaperImmunisation with Neospora caninum subunits rsNcSAG4 and rsNcGRA1 (NcSAG4 and NcGRA1 epitopes construct) in BALB/c mice: the profile of the immune response and controlling the vertical transmission
Fidelis, Cintia Fernandes, Araújo, Leandro Silva de, Prates-Patarroyo, Pablo A., Martins-Kalks, Karlos H., Oliveira, Leandro Licursi de, Vilória, Marlene Isabel Vargas, Tafur-Gómez, Gabriel Andrés, Salcedo, Joaquín Hernán Patarroyo · 2023 · Parasitology Research · DOI
Full title: Immunisation with Neospora caninum subunits rsNcSAG4 and rsNcGRA1 (NcSAG4 and NcGRA1 epitopes construct) in BALB/c mice: the profile of the immune response and controlling the vertical transmission
Authors: Fidelis, Cintia Fernandes, Araújo, Leandro Silva de, Prates-Patarroyo, Pablo A., Martins-Kalks, Karlos H., Oliveira, Leandro Licursi de, Vilória, Marlene Isabel Vargas, Tafur-Gómez, Gabriel Andrés, Salcedo, Joaquín Hernán Patarroyo
PaperAdaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae
Espinosa, Monica I., González-García, R. Axayácatl, Valgepea, Kaspar, Plan, Manuel R., Scott, Colin, Pretorius, Isak S., Marcellin, Esteban, Paulsen, Ian T., Williams, Thomas C. · 2020 · Nature Communications · DOI
Full title: Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae
Authors: Espinosa, Monica I., González-García, R. Axayácatl, Valgepea, Kaspar, Plan, Manuel R., Scott, Colin, Pretorius, Isak S., Marcellin, Esteban, Paulsen, Ian T., Williams, Thomas C.
PaperIdentification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Yu, Xiao‐Wei, Sun, Weihong, Wang, Ying-Zheng, Xu, Yan · 2017 · Scientific Reports · DOI
Full title: Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Authors: Yu, Xiao‐Wei, Sun, Weihong, Wang, Ying-Zheng, Xu, Yan
PaperStructural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells
C. R. Arbeitman, G. Auge, M. Blaustein, L. Bredeston, Enrique S. Corapi, P. Craig, L. A. Cossio, L. Dain, C. D’Alessio, Fernanda Elias, Natalia B Fernández, J. Gasulla, Natalia Gorojovsky, Gustavo E. Gudesblat, Maria Georgina Herrera, L. I. Ibañez, Tommy Idrovo, Matías Iglesias Randon, L. Kamenetzky, A. Nadra, D. Noseda, C. Pavan, María F. Pavan, M. F. Pignataro, Ernesto A. Román, L. Ruberto, N. Rubinstein, Javier Santos, F. Duarte, A. Zelada · 2020 · Scientific Reports · DOI
Full title: Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells
Authors: C. R. Arbeitman, G. Auge, M. Blaustein, L. Bredeston, Enrique S. Corapi, P. Craig, L. A. Cossio, L. Dain, C. D’Alessio, Fernanda Elias, Natalia B Fernández, J. Gasulla, Natalia Gorojovsky, Gustavo E. Gudesblat, Maria Georgina Herrera, L. I. Ibañez, Tommy Idrovo, Matías Iglesias Randon, L. Kamenetzky, A. Nadra, D. Noseda, C. Pavan, María F. Pavan, M. F. Pignataro, Ernesto A. Román, L. Ruberto, N. Rubinstein, Javier Santos, F. Duarte, A. Zelada
PaperPredicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden
Wollborn, David, Munkler, Lara Pauline, Horstmann, Rebekka, Germer, Andrea, Blank, Lars M., Büchs, Jochen · 2022 · Scientific Reports · DOI
Full title: Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden
Authors: Wollborn, David, Munkler, Lara Pauline, Horstmann, Rebekka, Germer, Andrea, Blank, Lars M., Büchs, Jochen
PaperA DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineering
Yuzbashev, Tigran V., Yuzbasheva, Evgeniya Y., Melkina, O. E., Patel, Davina, Бубнов, Д. М., Dietz, Heiko, Ledesma‐Amaro, Rodrigo · 2023 · Communications Biology · DOI
Full title: A DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineering
Authors: Yuzbashev, Tigran V., Yuzbasheva, Evgeniya Y., Melkina, O. E., Patel, Davina, Бубнов, Д. М., Dietz, Heiko, Ledesma‐Amaro, Rodrigo
PaperScaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement.
Full title: Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement.
PaperMethanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris.
Full title: Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris.
PaperDevelopment of a Pichia pastoris whole-cell biocatalyst with overexpression of mutant lipase I PCLG47I from Penicillium cyclopium for biodiesel production.
Full title: Development of a Pichia pastoris whole-cell biocatalyst with overexpression of mutant lipase I PCLG47I from Penicillium cyclopium for biodiesel production.
PaperExpression of <sup>2</sup>H, <sup>13</sup>C, <sup>15</sup>N-labeled NIST-Fab fragment in the methylotrophic yeast <i>Komagataella phaffii</i> for nuclear magnetic resonance studies
Chao, Kinlin, O’Dell, William B., Solomon, Tsega L., Brinson, Robert G., Marino, John P., Kelman, Zvi · 2023 · EPJ Web of Conferences · DOI
Full title: Expression of <sup>2</sup>H, <sup>13</sup>C, <sup>15</sup>N-labeled NIST-Fab fragment in the methylotrophic yeast <i>Komagataella phaffii</i> for nuclear magnetic resonance studies
Authors: Chao, Kinlin, O’Dell, William B., Solomon, Tsega L., Brinson, Robert G., Marino, John P., Kelman, Zvi
PaperA combined flow injection/reversed phase chromatography – high resolution mass spectrometry workflow for accurate absolute lipid quantification with <sup>13</sup> C- internal standards
Schoeny, Harald, Rampler, Evelyn, Abiead, Yasin El, Hildebrand, Felina, Zach, Olivia, Hermann, Gerrit, Koellensperger, Gunda · 2020 · DOI
Full title: A combined flow injection/reversed phase chromatography – high resolution mass spectrometry workflow for accurate absolute lipid quantification with <sup>13</sup> C- internal standards
PaperLyGo: A platform for rapid screening of lytic polysaccharide monooxygenase production
Hernández‐Rollán, Cristina, Falkenberg, Kristoffer Bach, Rennig, Maja, Bertelsen, Andreas Birk, Ipsen, Johan Ø., Brander, Søren, Daley, Daniel O., Johansen, Katja S., Nørholm, Morten H. H. · 2020 · DOI
Full title: LyGo: A platform for rapid screening of lytic polysaccharide monooxygenase production
Authors: Hernández‐Rollán, Cristina, Falkenberg, Kristoffer Bach, Rennig, Maja, Bertelsen, Andreas Birk, Ipsen, Johan Ø., Brander, Søren, Daley, Daniel O., Johansen, Katja S., Nørholm, Morten H. H.
PaperMolecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher · 2020 · DOI
Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Authors: Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher
PaperEngineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters
Dalvie, Neil C., Rodriguez‐Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Célia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dong Soo, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanné, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith M., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice, Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher · 2021 · DOI
Full title: Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters
Authors: Dalvie, Neil C., Rodriguez‐Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Célia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dong Soo, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanné, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith M., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice, Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher
PaperScalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii.
Dalvie, Neil C, Biedermann, Andrew M, Rodriguez-Aponte, Sergio A, Naranjo, Christopher A, Rao, Harish D, Rajurkar, Meghraj P, Lothe, Rakesh R, Shaligram, Umesh S, Johnston, Ryan S, Crowell, Laura E, Castelino, Seraphin, Tracey, Mary Kate, Whittaker, Charles A, Love, J Christopher · 2021 · bioRxiv : the preprint server for biology · DOI
Full title: Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii.
Authors: Dalvie, Neil C, Biedermann, Andrew M, Rodriguez-Aponte, Sergio A, Naranjo, Christopher A, Rao, Harish D, Rajurkar, Meghraj P, Lothe, Rakesh R, Shaligram, Umesh S, Johnston, Ryan S, Crowell, Laura E, Castelino, Seraphin, Tracey, Mary Kate, Whittaker, Charles A, Love, J Christopher
PaperP <sub> <i>AOX1</i> </sub> expression in mixed-substrate continuous cultures of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) is completely determined by methanol consumption regardless of the secondary carbon source
Full title: P <sub> <i>AOX1</i> </sub> expression in mixed-substrate continuous cultures of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) is completely determined by methanol consumption regardless of the secondary carbon source
PaperSteric accessibility of the <i>N</i> -terminus improves the titer and quality of recombinant proteins secreted from <i>Komagataella phaffii</i>
Dalvie, Neil C., Naranjo, Christopher A., Rodriguez‐Aponte, Sergio A., Johnston, Ryan S., Love, J. Christopher · 2022 · DOI
Full title: Steric accessibility of the <i>N</i> -terminus improves the titer and quality of recombinant proteins secreted from <i>Komagataella phaffii</i>
Authors: Dalvie, Neil C., Naranjo, Christopher A., Rodriguez‐Aponte, Sergio A., Johnston, Ryan S., Love, J. Christopher
PaperFlux balance analysis predicts NADP phosphatase and NADH kinase are critical to balancing redox during xylose fermentation in <i>Scheffersomyces stipitis</i>
Correia, Kevin, Khusnutdinova, Anna N., Li, Peter Yan, Joo, Jeong Chan, Brown, Greg, Yakunin, Alexander F., Mahadevan, Radhakrishnan · 2018 · DOI
Full title: Flux balance analysis predicts NADP phosphatase and NADH kinase are critical to balancing redox during xylose fermentation in <i>Scheffersomyces stipitis</i>
Authors: Correia, Kevin, Khusnutdinova, Anna N., Li, Peter Yan, Joo, Jeong Chan, Brown, Greg, Yakunin, Alexander F., Mahadevan, Radhakrishnan
PaperThe Mut <sup>+</sup> strain of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) expresses P <i> <sub>AOX1</sub> </i> 5 and 10 times faster than Mut <sup>s</sup> and Mut <sup>−</sup> strains: Evidence that formaldehyde or/and formate are true inducers of AOX
Full title: The Mut <sup>+</sup> strain of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) expresses P <i> <sub>AOX1</sub> </i> 5 and 10 times faster than Mut <sup>s</sup> and Mut <sup>−</sup> strains: Evidence that formaldehyde or/and formate are true inducers of AOX
PaperGenome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1
Ravin, Nikolai V., Eldarov, Michael A., Kadnikov, Vitaly V., Beletsky, Alexey V., Schneider, Jessica, Mardanova, Eugenia S., Smekalova, Elena M., Zvereva, Maria I., Донцова, О. А., Mardanov, Andrey V., Skryabin, K. G. · 2013 · BMC Genomics · DOI
Full title: Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1
Authors: Ravin, Nikolai V., Eldarov, Michael A., Kadnikov, Vitaly V., Beletsky, Alexey V., Schneider, Jessica, Mardanova, Eugenia S., Smekalova, Elena M., Zvereva, Maria I., Донцова, О. А., Mardanov, Andrey V., Skryabin, K. G.
PaperComprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase.
Hemmerich, Johannes, Adelantado, Núria, Barrigón, José Manuel, Ponte, Xavier, Hörmann, Astrid, Ferrer, Pau, Kensy, Frank, Valero, Francisco · 2014 · Microbial cell factories · DOI
Full title: Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase.
Authors: Hemmerich, Johannes, Adelantado, Núria, Barrigón, José Manuel, Ponte, Xavier, Hörmann, Astrid, Ferrer, Pau, Kensy, Frank, Valero, Francisco
PaperDeveloping a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime.
Holmes, William J, Darby, Richard Aj, Wilks, Martin Db, Smith, Rodney, Bill, Roslyn M · 2009 · Microbial cell factories · DOI
Full title: Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime.
Authors: Holmes, William J, Darby, Richard Aj, Wilks, Martin Db, Smith, Rodney, Bill, Roslyn M
PaperImproved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel.
Huang, Jinjin, Xia, Ji, Yang, Zhen, Guan, Feifei, Cui, Di, Guan, Guohua, Jiang, Wei, Li, Ying · 2014 · Biotechnology for biofuels · DOI
Full title: Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel.
PaperPichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level
Prielhofer, Roland, Cartwright, Stephanie P., Graf, Alexandra B., Valli, Minoska, Bill, Roslyn M., Mattanovich, Diethard, Gasser, Brigitte · 2015 · BMC Genomics · DOI
Full title: Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level
Authors: Prielhofer, Roland, Cartwright, Stephanie P., Graf, Alexandra B., Valli, Minoska, Bill, Roslyn M., Mattanovich, Diethard, Gasser, Brigitte
PaperComparative genomics and transcriptomics of Pichia pastoris
Love, Kerry R., Shah, Kartik, Whittaker, Charles A., Wu, Jie, Bartlett, M. Catherine, Ma, Duanduan, Leeson, Rachel, Priest, Margaret, Borowsky, Jonathan, Young, Sarah, Love, J. Christopher · 2016 · BMC Genomics · DOI
Full title: Comparative genomics and transcriptomics of Pichia pastoris
Authors: Love, Kerry R., Shah, Kartik, Whittaker, Charles A., Wu, Jie, Bartlett, M. Catherine, Ma, Duanduan, Leeson, Rachel, Priest, Margaret, Borowsky, Jonathan, Young, Sarah, Love, J. Christopher
PaperOptimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33.
Full title: Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33.
PaperAn effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)
Che, Zhiqun, Cao, Xiaoyan, Chen, Guiguang, Liang, Zhiqun · 2020 · BMC Biotechnology · DOI
Full title: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)
Authors: Che, Zhiqun, Cao, Xiaoyan, Chen, Guiguang, Liang, Zhiqun
PaperGenome-scale model reconstruction of the methylotrophic yeast Ogataea polymorpha
Liebal, Ulf W., Fabry, Brigida A, Ravikrishnan, Aarthi, Schedel, Constantin VL, Schmitz, Simone, Blank, Lars M., Ebert, Birgitta E. · 2021 · BMC Biotechnology · DOI
Full title: Genome-scale model reconstruction of the methylotrophic yeast Ogataea polymorpha
Authors: Liebal, Ulf W., Fabry, Brigida A, Ravikrishnan, Aarthi, Schedel, Constantin VL, Schmitz, Simone, Blank, Lars M., Ebert, Birgitta E.
PaperImplications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.
Moser, Josef W, Prielhofer, Roland, Gerner, Samuel M, Graf, Alexandra B, Wilson, Iain B H, Mattanovich, Diethard, Dragosits, Martin · 2017 · Microbial cell factories · DOI
Full title: Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.
Authors: Moser, Josef W, Prielhofer, Roland, Gerner, Samuel M, Graf, Alexandra B, Wilson, Iain B H, Mattanovich, Diethard, Dragosits, Martin
Full title: Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris
PaperSpecific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage
Garrigós‐Martínez, Javier, Nieto‐Taype, Miguel Angel, Gasset-Franch, Arnau, Montesinos, José Luis González, Garcia‐Ortega, Xavier, Valero, Francisco · 2019 · Microbial Cell Factories · DOI
Full title: Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage
Authors: Garrigós‐Martínez, Javier, Nieto‐Taype, Miguel Angel, Gasset-Franch, Arnau, Montesinos, José Luis González, Garcia‐Ortega, Xavier, Valero, Francisco
Full title: Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc
PaperMolecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher · 2021 · Microbial Cell Factories · DOI
Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Authors: Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher
PaperBiosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris
Navone, Laura, Moffitt, Kaylee, Behrendorff, James B. Y. H., Sadowski, Paweł, Hartley, Carol J., Speight, Robert · 2023 · Microbial Cell Factories · DOI
Full title: Biosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris
Authors: Navone, Laura, Moffitt, Kaylee, Behrendorff, James B. Y. H., Sadowski, Paweł, Hartley, Carol J., Speight, Robert
PaperHigh throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux
Full title: High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux
PaperImpact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications
Full title: Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications
Full title: Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii
PaperChaetomella raphigera β-glucosidase D2-BGL has intriguing structural features and a high substrate affinity that renders it an efficient cellulase supplement for lignocellulosic biomass hydrolysis.
Kao, Mu-Rong, Kuo, Hsion-Wen, Lee, Cheng-Chung, Huang, Kuan-Ying, Huang, Ting-Yen, Li, Chen-Wei, Chen, C Will, Wang, Andrew H-J, Yu, Su-May, Ho, Tuan-Hua David · 2019 · Biotechnology for biofuels · DOI
Full title: Chaetomella raphigera β-glucosidase D2-BGL has intriguing structural features and a high substrate affinity that renders it an efficient cellulase supplement for lignocellulosic biomass hydrolysis.
Authors: Kao, Mu-Rong, Kuo, Hsion-Wen, Lee, Cheng-Chung, Huang, Kuan-Ying, Huang, Ting-Yen, Li, Chen-Wei, Chen, C Will, Wang, Andrew H-J, Yu, Su-May, Ho, Tuan-Hua David
PaperPhysiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors
Paes, Barbara G., Steindorff, Andrei Stecca, Formighieri, Eduardo F., Pereira, Ildinete Silva, Almeida, João Ricardo Moreira de · 2021 · AMB Express · DOI
Full title: Physiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-derived inhibitors
Authors: Paes, Barbara G., Steindorff, Andrei Stecca, Formighieri, Eduardo F., Pereira, Ildinete Silva, Almeida, João Ricardo Moreira de
PaperImproved microscale cultivation of Pichia pastoris for clonal screening.
Eck, Alexander, Schmidt, Matthias, Hamer, Stefanie, Ruff, Anna Joelle, Förster, Jan, Schwaneberg, Ulrich, Blank, Lars M, Wiechert, Wolfgang, Oldiges, Marco · 2018 · Fungal biology and biotechnology · DOI
Full title: Improved microscale cultivation of Pichia pastoris for clonal screening.
Authors: Eck, Alexander, Schmidt, Matthias, Hamer, Stefanie, Ruff, Anna Joelle, Förster, Jan, Schwaneberg, Ulrich, Blank, Lars M, Wiechert, Wolfgang, Oldiges, Marco
PaperEnhanced Co-culture System Using <i>Escherichia coli</i> and <i>Pichia pastoris</i> (<i>Komagataella phaffii</i>) for Improved Microbial Production of Valuable Plant Alkaloids
Full title: Enhanced Co-culture System Using <i>Escherichia coli</i> and <i>Pichia pastoris</i> (<i>Komagataella phaffii</i>) for Improved Microbial Production of Valuable Plant Alkaloids
PaperIn silico and in vitro analysis of THCA synthase gene in Moroccan Cannabis sativa, L
Badrana, Fadwa, Fahime, El Mostafa El, Mokhtari, A., Soulaymani, Abdelmajid, Safini, Najete, Chaouni, Bouchra, Malka, Gabriel, Amri, Hamid El · 2022 · F1000Research · DOI
Full title: In silico and in vitro analysis of THCA synthase gene in Moroccan Cannabis sativa, L
Authors: Badrana, Fadwa, Fahime, El Mostafa El, Mokhtari, A., Soulaymani, Abdelmajid, Safini, Najete, Chaouni, Bouchra, Malka, Gabriel, Amri, Hamid El
PaperStructure of SALO, a leishmaniasis vaccine candidate from the sand fly Lutzomyia longipalpis.
Asojo, Oluwatoyin A, Kelleher, Alan, Liu, Zhuyun, Pollet, Jeroen, Hudspeth, Elissa M, Rezende, Wanderson C, Groen, Mallory Jo, Seid, Christopher A, Abdeladhim, Maha, Townsend, Shannon, de Castro, Waldione, Mendes-Sousa, Antonio, Bartholomeu, Daniella Castanheira, Fujiwara, Ricardo Toshio, Bottazzi, Maria Elena, Hotez, Peter J, Zhan, Bin, Oliveira, Fabiano, Kamhawi, Shaden, Valenzuela, Jesus G · 2017 · PLoS neglected tropical diseases · DOI
Full title: Structure of SALO, a leishmaniasis vaccine candidate from the sand fly Lutzomyia longipalpis.
Authors: Asojo, Oluwatoyin A, Kelleher, Alan, Liu, Zhuyun, Pollet, Jeroen, Hudspeth, Elissa M, Rezende, Wanderson C, Groen, Mallory Jo, Seid, Christopher A, Abdeladhim, Maha, Townsend, Shannon, de Castro, Waldione, Mendes-Sousa, Antonio, Bartholomeu, Daniella Castanheira, Fujiwara, Ricardo Toshio, Bottazzi, Maria Elena, Hotez, Peter J, Zhan, Bin, Oliveira, Fabiano, Kamhawi, Shaden, Valenzuela, Jesus G
PaperConformational and thermal stability improvements for the large-scale production of yeast-derived rabbit hemorrhagic disease virus-like particles as multipurpose vaccine.
Fernández, Erlinda, Toledo, Jorge R, Méndez, Lídice, González, Nemecio, Parra, Francisco, Martín-Alonso, José M, Limonta, Miladys, Sánchez, Kosara, Cabrales, Ania, Estrada, Mario P, Rodríguez-Mallón, Alina, Farnós, Omar · 2013 · PloS one · DOI
Full title: Conformational and thermal stability improvements for the large-scale production of yeast-derived rabbit hemorrhagic disease virus-like particles as multipurpose vaccine.
Authors: Fernández, Erlinda, Toledo, Jorge R, Méndez, Lídice, González, Nemecio, Parra, Francisco, Martín-Alonso, José M, Limonta, Miladys, Sánchez, Kosara, Cabrales, Ania, Estrada, Mario P, Rodríguez-Mallón, Alina, Farnós, Omar
PaperTranslational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
Edwards-Jones, Bryn, Aw, Rochelle, Barton, Geraint, Tredwell, Gregory D., Bundy, Jacob G., Leak, David J. · 2015 · PLoS ONE · DOI
Full title: Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
Authors: Edwards-Jones, Bryn, Aw, Rochelle, Barton, Geraint, Tredwell, Gregory D., Bundy, Jacob G., Leak, David J.
PaperIntegration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism
Tomàs‐Gamisans, Màrius, Ferrer, Pau, Albiol, Joan · 2016 · PLoS ONE · DOI
Full title: Integration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism
Authors: Tomàs‐Gamisans, Màrius, Ferrer, Pau, Albiol, Joan
PaperGeneration of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants
Detering, Thorben, Mundry, Katharina, Berger, Ralf G. · 2020 · PLoS ONE · DOI
Full title: Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants
Authors: Detering, Thorben, Mundry, Katharina, Berger, Ralf G.
PaperPopulation genomics shows no distinction between pathogenic Candida krusei and environmental Pichia kudriavzevii: One species, four names
Douglass, Alexander, Offei, Benjamin, Braun‐Galleani, Stephanie, Coughlan, Aisling Y., Martos, Alexandre A. R., Ortiz‐Merino, Raúl A., Byrne, Kevin P., Wolfe, Kenneth H. · 2018 · PLoS Pathogens · DOI
Full title: Population genomics shows no distinction between pathogenic Candida krusei and environmental Pichia kudriavzevii: One species, four names
Authors: Douglass, Alexander, Offei, Benjamin, Braun‐Galleani, Stephanie, Coughlan, Aisling Y., Martos, Alexandre A. R., Ortiz‐Merino, Raúl A., Byrne, Kevin P., Wolfe, Kenneth H.
PaperApplications of recombinant Pichia pastoris in the healthcare industry.
Weinacker, Daniel, Rabert, Claudia, Zepeda, Andrea B, Figueroa, Carolina A, Pessoa, Adalberto, Farías, Jorge G · 2013 · Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · DOI
Full title: Applications of recombinant Pichia pastoris in the healthcare industry.
Authors: Weinacker, Daniel, Rabert, Claudia, Zepeda, Andrea B, Figueroa, Carolina A, Pessoa, Adalberto, Farías, Jorge G
Year: 2013
Venue: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
PaperConstitutive High Expression Level of A Synthetic Deleted Encoding Gene of <i>Talaromyces minioluteus</i> Endodextranase Variant (r-TmDEX49A-ΔSP-ΔN30) in <i>Komagataella phaffii</i> (<i>Pichia pastoris</i>)
Full title: Constitutive High Expression Level of A Synthetic Deleted Encoding Gene of <i>Talaromyces minioluteus</i> Endodextranase Variant (r-TmDEX49A-ΔSP-ΔN30) in <i>Komagataella phaffii</i> (<i>Pichia pastoris</i>)
PaperDevelopment of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain (RBD) Using Pichia pastoris in Stirred-Tank Bioreactor
Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, María Florencia, Wetzler, Diana E., Gentilli, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas AM · 2023 · Preprints.org · DOI
Full title: Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain (RBD) Using Pichia pastoris in Stirred-Tank Bioreactor
Authors: Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, María Florencia, Wetzler, Diana E., Gentilli, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas AM
Full title: Production of Plant-Based Meat Flavor Compound – Leghemo-Globin (LegH) in Recombinant <em>Komagataella phaffii</em> Fed-Batch Fermentations
PaperState of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
Full title: State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
PaperEstablishing site-specific recombination mediated cassette exchange in Pichia pastoris and development of an optimized process for the production of mouse Tmprss2
Schinkowski, Christian · 2017 · New Trends in Mathematical Science · DOI
Full title: Establishing site-specific recombination mediated cassette exchange in Pichia pastoris and development of an optimized process for the production of mouse Tmprss2
PaperOverall Key Performance Indicator to Optimizing Operation of High-Pressure Homogenizers for a Reliable Quantification of Intracellular Components in Pichia pastoris.
Garcia-Ortega, Xavier, Reyes, Cecilia, Montesinos, José Luis, Valero, Francisco · 2015 · Frontiers in bioengineering and biotechnology · DOI
Full title: Overall Key Performance Indicator to Optimizing Operation of High-Pressure Homogenizers for a Reliable Quantification of Intracellular Components in Pichia pastoris.
Authors: Garcia-Ortega, Xavier, Reyes, Cecilia, Montesinos, José Luis, Valero, Francisco
Year: 2015
Venue: Frontiers in bioengineering and biotechnology
PaperContinuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory
Miguel Angel Nieto-Taype, Xavier Garcia-Ortega, J. Albiol, José Luis Montesinos-Seguí, F. Valero · 2020 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory
Authors: Miguel Angel Nieto-Taype, Xavier Garcia-Ortega, J. Albiol, José Luis Montesinos-Seguí, F. Valero
Year: 2020
Venue: Frontiers in Bioengineering and Biotechnology
PaperInnovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
Gasset, Arnau, Garcia‐Ortega, Xavier, Garrigós‐Martínez, Javier, Valero, Francisco, Montesinos, José Luis González · 2022 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
Authors: Gasset, Arnau, Garcia‐Ortega, Xavier, Garrigós‐Martínez, Javier, Valero, Francisco, Montesinos, José Luis González
Year: 2022
Venue: Frontiers in Bioengineering and Biotechnology
PaperEnantioselective Biosynthesis of L-Phenyllactic Acid From Phenylpyruvic Acid In Vitro by L-Lactate Dehydrogenase Coupling With Glucose Dehydrogenase.
Zhang, Dong, Zhang, Ting, Lei, Yuqing, Lin, Wenqian, Chen, Xingyi, Wu, Minchen · 2022 · Frontiers in bioengineering and biotechnology · DOI
Full title: Enantioselective Biosynthesis of L-Phenyllactic Acid From Phenylpyruvic Acid In Vitro by L-Lactate Dehydrogenase Coupling With Glucose Dehydrogenase.
PaperPAOX1 expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determined by methanol consumption regardless of the secondary carbon source
Singh, Anamika, Narang, Atul · 2023 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: PAOX1 expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determined by methanol consumption regardless of the secondary carbon source
Authors: Singh, Anamika, Narang, Atul
Year: 2023
Venue: Frontiers in Bioengineering and Biotechnology
PaperNitrogen recovery from low-value biogenic feedstocks via steam gasification to methylotrophic yeast biomass
Shirvani, Roghayeh, Bartik, Alexander, Alves, Gustavo A. S., Hernandez, Daniel Garcia de Otazo, Müller, Stefan, Föttinger, Karin, Steiger, Matthias G. · 2023 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Nitrogen recovery from low-value biogenic feedstocks via steam gasification to methylotrophic yeast biomass
Authors: Shirvani, Roghayeh, Bartik, Alexander, Alves, Gustavo A. S., Hernandez, Daniel Garcia de Otazo, Müller, Stefan, Föttinger, Karin, Steiger, Matthias G.
Year: 2023
Venue: Frontiers in Bioengineering and Biotechnology
PaperEngineering the methylotrophic yeast Ogataea polymorpha for lactate production from methanol
Wefelmeier, Katrin, Schmitz, Simone, Haut, Anna Maria, Otten, Johannes, Jülich, Tobias, Blank, Lars M. · 2023 · Frontiers in Bioengineering and Biotechnology · DOI
Full title: Engineering the methylotrophic yeast Ogataea polymorpha for lactate production from methanol
Authors: Wefelmeier, Katrin, Schmitz, Simone, Haut, Anna Maria, Otten, Johannes, Jülich, Tobias, Blank, Lars M.
Year: 2023
Venue: Frontiers in Bioengineering and Biotechnology
PaperSingle-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes With Pichia pastoris
Hana Raschmanová, Iwo Zamora, Martina Borčinová, Patrick Meier, A. Weninger, Dominik Mächler, A. Glieder, K. Melzoch, Z. Knejzlík, K. Kovar · 2019 · Frontiers in Microbiology · DOI
Full title: Single-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes With Pichia pastoris
Authors: Hana Raschmanová, Iwo Zamora, Martina Borčinová, Patrick Meier, A. Weninger, Dominik Mächler, A. Glieder, K. Melzoch, Z. Knejzlík, K. Kovar
PaperComparative transcriptomic analysis-based identification of the regulation of foreign proteins with different stabilities expressed in Pichia pastoris
Niu, Tingting, Cui, Yi, Xu, Shan, Qin, Shuzhen, Zhou, Xuejie, Wang, Rui, Chang, Alan K., Ma, Nan, Jing, Jingjing, He, Jianwei · 2022 · Frontiers in Microbiology · DOI
Full title: Comparative transcriptomic analysis-based identification of the regulation of foreign proteins with different stabilities expressed in Pichia pastoris
PaperMultiple strategies were adopted to optimize the enzymatic characteristics and improve the expression of bovine chymosin BtChy in Kluyveromyces lactis for cheese production
Han, Ying, Zhang, Lin, Rao, Deming, Lei, Lei, Yang, Jiangke · 2025 · Frontiers in Microbiology · DOI
Full title: Multiple strategies were adopted to optimize the enzymatic characteristics and improve the expression of bovine chymosin BtChy in Kluyveromyces lactis for cheese production
PaperFrom linoleic acid to hexanal and hexanol by whole cell catalysis with a lipoxygenase, hydroperoxide lyase and reductase cascade in Komagataella phaffii
Hashem, Chiam, Hochrinner, Julius, Bürgler, Moritz B., Rinnofner, Claudia, Pichler, Harald, Winkler, Margit · 2022 · Frontiers in Molecular Biosciences · DOI
Full title: From linoleic acid to hexanal and hexanol by whole cell catalysis with a lipoxygenase, hydroperoxide lyase and reductase cascade in Komagataella phaffii
Authors: Hashem, Chiam, Hochrinner, Julius, Bürgler, Moritz B., Rinnofner, Claudia, Pichler, Harald, Winkler, Margit
PaperBoosting Expression of a Specifically Targeted Antimicrobial Peptide K in Pichia pastoris by Employing a 2A Self-Cleaving Peptide-Based Expression System.
Full title: Boosting Expression of a Specifically Targeted Antimicrobial Peptide K in Pichia pastoris by Employing a 2A Self-Cleaving Peptide-Based Expression System.
PaperEffect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineered for Lactic Acid Production
Melo, Nadiele Tamires Moreira, Mulder, Kelly C.L., Nicola, André Moraes, Carvalho, Lucas Silva, Menino, Gisele, Mulinari, Eduardo, Parachin, Nádia Skorupa · 2018 · Bioengineering · DOI
Full title: Effect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineered for Lactic Acid Production
Authors: Melo, Nadiele Tamires Moreira, Mulder, Kelly C.L., Nicola, André Moraes, Carvalho, Lucas Silva, Menino, Gisele, Mulinari, Eduardo, Parachin, Nádia Skorupa
PaperPreparative Production of Functionalized (N- and O-Heterocyclic) Polycyclic Aromatic Hydrocarbons by Human Cytochrome P450 3A4 in a Bioreactor
Srdič, Matic, Fessner, Nico D., Yıldız, Deniz, Glieder, Anton, Spiertz, Markus, Schwaneberg, Ulrich · 2022 · Biomolecules · DOI
Full title: Preparative Production of Functionalized (N- and O-Heterocyclic) Polycyclic Aromatic Hydrocarbons by Human Cytochrome P450 3A4 in a Bioreactor
PaperStatistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris
Maity, Nitu, Mishra, Saroj · 2019 · Biomolecules · DOI
Full title: Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris
PaperExpression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi.
Birchfield, Aaron S, McIntosh, Cecilia A · 2024 · Biotech (Basel (Switzerland)) · DOI
Full title: Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi.
PaperPerformance of Recombinant Komagataella phaffii in Plant-Based Meat Flavor Compound-Leghemoglobin (LegH) Production through Fed-Batch Fermentations
Full title: Performance of Recombinant Komagataella phaffii in Plant-Based Meat Flavor Compound-Leghemoglobin (LegH) Production through Fed-Batch Fermentations
PaperState of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
Full title: State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
PaperEnhancing Antimicrobial Peptide Productivity in Pichia pastoris (Muts Strain) by Improving the Fermentation Process Based on Increasing the Volumetric Methanol Consumption Rate
Full title: Enhancing Antimicrobial Peptide Productivity in Pichia pastoris (Muts Strain) by Improving the Fermentation Process Based on Increasing the Volumetric Methanol Consumption Rate
PaperDevelopment of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain Using Pichia pastoris in Stirred-Tank Bioreactor
Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, Florencia, Wetzler, Diana E., Gentili, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas · 2023 · Fermentation · DOI
Full title: Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain Using Pichia pastoris in Stirred-Tank Bioreactor
Authors: Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, Florencia, Wetzler, Diana E., Gentili, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas
PaperTranscriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3
Full title: Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3
PaperHeterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
Zadorozhny, A. V., Ushakov, Viktor Sergeevich, Розанов, А. С., Bogacheva, Natalia Vladimirovna, Shlyakhtun, Valeria Nikolayevna, Voskoboev, Mikhail Evgenyevich, Korzhuk, Anton V., Romancev, Vladislav Anatolevich, Bannikova, Svetlana V., Mescheryakova, Irina Anatolyevna, Антонов, Е. В., Vasilieva, Asya R., Pavlova, Elena Iurevna, Chesnokov, Danil Olegovich, Shedko, Elizaveta Dmitrievna, Bryanskaya, A. V., Bochkov, D. V., Goryachkovskaya, T. N., Peltek, S. E. · 2022 · International Journal of Molecular Sciences · DOI
Full title: Heterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
Authors: Zadorozhny, A. V., Ushakov, Viktor Sergeevich, Розанов, А. С., Bogacheva, Natalia Vladimirovna, Shlyakhtun, Valeria Nikolayevna, Voskoboev, Mikhail Evgenyevich, Korzhuk, Anton V., Romancev, Vladislav Anatolevich, Bannikova, Svetlana V., Mescheryakova, Irina Anatolyevna, Антонов, Е. В., Vasilieva, Asya R., Pavlova, Elena Iurevna, Chesnokov, Danil Olegovich, Shedko, Elizaveta Dmitrievna, Bryanskaya, A. V., Bochkov, D. V., Goryachkovskaya, T. N., Peltek, S. E.
Year: 2022
Venue: International Journal of Molecular Sciences
PaperProduction of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unveiling Antimicrobial and Anticancer Functionalities.
Yen, Chih-Ching, Wu, Pei-Ying, Ou-Yang, Huan, Chen, Hsiao-Ling, Chong, Kowit-Yu, Chang, Ro-Lin, Chen, Chuan-Mu · 2024 · International journal of molecular sciences · DOI
Full title: Production of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unveiling Antimicrobial and Anticancer Functionalities.
PaperKomagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Хлебодарова, Т. М., Bogacheva, N. V., Zadorozhny, A. V., Bryanskaya, A. V., Vasilieva, Asya R., Chesnokov, Danil Olegovich, Pavlova, Elena I., Peltek, S. E. · 2024 · Microorganisms · DOI
Full title: Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Authors: Хлебодарова, Т. М., Bogacheva, N. V., Zadorozhny, A. V., Bryanskaya, A. V., Vasilieva, Asya R., Chesnokov, Danil Olegovich, Pavlova, Elena I., Peltek, S. E.
PaperRecombinant Inga Laurina Trypsin Inhibitor (ILTI) Production in Komagataella Phaffii Confirms Its Potential Anti-Biofilm Effect and Reveals an Anti-Tumoral Activity.
Carneiro, Fábio C, Weber, Simone S, Silva, Osmar N, Jacobowski, Ana Cristina, Ramada, Marcelo H S, Macedo, Maria L R, Franco, Octávio L, Parachin, Nádia S · 2018 · Microorganisms · DOI
Full title: Recombinant Inga Laurina Trypsin Inhibitor (ILTI) Production in Komagataella Phaffii Confirms Its Potential Anti-Biofilm Effect and Reveals an Anti-Tumoral Activity.
Authors: Carneiro, Fábio C, Weber, Simone S, Silva, Osmar N, Jacobowski, Ana Cristina, Ramada, Marcelo H S, Macedo, Maria L R, Franco, Octávio L, Parachin, Nádia S
PaperEvaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Komagataella phaffii Strains Utilizing Glycerol as Substrate
Melo, Nadielle Tamires Moreira, Pontes, Gabriela Coimbra, Procópio, Dielle Pierotti, Cunha, Gabriel Caetano de Góis e, Eliodório, Kevy Pontes, Paes, Hugo Costa, Basso, Thiago Olitta, Parachin, Nádia Skorupa · 2020 · Microorganisms · DOI
Full title: Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Komagataella phaffii Strains Utilizing Glycerol as Substrate
Authors: Melo, Nadielle Tamires Moreira, Pontes, Gabriela Coimbra, Procópio, Dielle Pierotti, Cunha, Gabriel Caetano de Góis e, Eliodório, Kevy Pontes, Paes, Hugo Costa, Basso, Thiago Olitta, Parachin, Nádia Skorupa
ThesisMetodologia para projeto de biorreatores industriais via otimização multiobjetivo com base em parâmetros de desempenho calculados por técnicas de CFD
Full title: Metodologia para projeto de biorreatores industriais via otimização multiobjetivo com base em parâmetros de desempenho calculados por técnicas de CFD
ThesisEvaluation of product distribution in chemostat and batch fermentation in lactic acid-producing komagataella phaffii strains utilizing glycerol as substrate
Full title: Evaluation of product distribution in chemostat and batch fermentation in lactic acid-producing komagataella phaffii strains utilizing glycerol as substrate
ThesisSimulation and techno-economic analysis of production processes of novel generation L-Asparaginases using recombinant Escherichia coli and Pichia pastoris
Azzoni, Adriano Rodrigues · 2022 · Universidade de São Paulo – USP Digital Library · DOI
Full title: Simulation and techno-economic analysis of production processes of novel generation L-Asparaginases using recombinant Escherichia coli and Pichia pastoris
Authors: Azzoni, Adriano Rodrigues
Year: 2022
Venue: Universidade de São Paulo – USP Digital Library
ThesisHigh-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
Blanchard, Véronique; Gadkari, R.P; George, A.V.E. et al. · 2008 · Utrecht University · DOI
Full title: High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
ThesisMolecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Dalvie, Neil C; Brady, Joseph R; Crowell, Laura E et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI
Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Authors: Dalvie, Neil C; Brady, Joseph R; Crowell, Laura E; Tracey, Mary K; Biedermann, Andrew M; Kaur, Kawaljit; Hickey, John M; Kristensen, D. L; Bonnyman, Alexandra D; Rodriguez-Aponte, Sergio A; Whittaker, Charles A; Bok, Marina; Vega, Celina; Mukhopadhyay, Tarit K; Joshi, Sangeeta B; Volkin, David B
Year: 2021
Venue: Massachusetts Institute of Technology (MIT)
ThesisOptimizing recombinant protein production in Chlamydomonas reinhardtii: turning a model organism into a commercially relevant biotechnological host
Torres-Tiji, Yasin · 2021 · University of California, San Diego (UCSD) · DOI
Full title: Optimizing recombinant protein production in Chlamydomonas reinhardtii: turning a model organism into a commercially relevant biotechnological host
ThesisDevelopment of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
Full title: Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
ThesisApplication of alternative fermenter design in whey-ethanol production (a preliminary study) : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Biotechnology at Massey University
Full title: Application of alternative fermenter design in whey-ethanol production (a preliminary study) : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Biotechnology at Massey University
ThesisEstudio de la dispersión multifásica y área interfacial de transferencia de masa a nivel local en un sistema modelo de fermentación micelial de tres fases
Full title: Estudio de la dispersión multifásica y área interfacial de transferencia de masa a nivel local en un sistema modelo de fermentación micelial de tres fases
Full title: Kinetic study of fed-batch and continuous bioconversion processes for L-Phenylacetylcarbinol (L-PAC) production by the yeast Candida utilis
ThesisModel-based real-time optimisation of a fed-batch cyanobacterial hydrogen production process using economic model predictive control strategy
del Rio-Chanona, Ehecatl Antonio; Zhang, Dongda; Vassiliadis, Vassilios S · 2015 · Theses · DOI
Full title: Model-based real-time optimisation of a fed-batch cyanobacterial hydrogen production process using economic model predictive control strategy
Authors: del Rio-Chanona, Ehecatl Antonio; Zhang, Dongda; Vassiliadis, Vassilios S
ThesisModel-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter
Barrigón de San Marcos, José Manuel · 2015 · Theses · DOI
Full title: Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter
ThesisUtilización de glicerol proveniente de la industria de biodiesel como fuente de carbono para la producción de lipasas recombinantes en Pichia pastoris
Full title: Utilización de glicerol proveniente de la industria de biodiesel como fuente de carbono para la producción de lipasas recombinantes en Pichia pastoris
ThesisA step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches
Full title: A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches
ThesisL’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic
Full title: L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic
Full title: Estratègies d’operació per a la producció de lipases de Rhizopus oryzae amb el fenotip Mut(s) de Pichia pastoris mitjançant substrats mixtes
ThesisCombining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris
Full title: Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris
Full title: Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris
ThesisPhysiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess
Full title: Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess
PaperAccurate and cost-effective prediction of HBsAg titer in industrial scale fermentation process of recombinant Pichia pastoris by using neural network based soft sensor.
Hosseini, Seyed Nezamedin, Javidanbardan, Amin, Khatami, Maryam · 2019 · Biotechnology and applied biochemistry · DOI
Full title: Accurate and cost-effective prediction of HBsAg titer in industrial scale fermentation process of recombinant Pichia pastoris by using neural network based soft sensor.
PaperCo‐Expression of Chaperones for Improvement of Recombinant <i>Geobacillus stearothermophilus</i> Lipase Production in <i>Pichia pastoris</i>
Erden‐Karaoğlan, Fidan · 2025 · Biotechnology and Applied Biochemistry · DOI
Full title: Co‐Expression of Chaperones for Improvement of Recombinant <i>Geobacillus stearothermophilus</i> Lipase Production in <i>Pichia pastoris</i>
PaperA New Strategy of Alternating Current Magnetic Field-Assisted Three-Phase Partitioning (ACMF-TPP) for the Concentration and Partial Purification of Recombinant Prepromelittin Produced by Komagataella phaffii.
Seyda Yildiz Arslan, F. Turhan, Kübra Solak, Hacer Karabulut, Melda Sisecioglu, Yagmur Unver · 2026 · Biotechnology and applied biochemistry · DOI
Full title: A New Strategy of Alternating Current Magnetic Field-Assisted Three-Phase Partitioning (ACMF-TPP) for the Concentration and Partial Purification of Recombinant Prepromelittin Produced by Komagataella phaffii.
Authors: Seyda Yildiz Arslan, F. Turhan, Kübra Solak, Hacer Karabulut, Melda Sisecioglu, Yagmur Unver
PaperMicroscale Perfusion‐Based Cultivation for <i>Pichia pastoris</i> Clone Screening Enables Accelerated and Optimized Recombinant Protein Production Processes
Full title: Microscale Perfusion‐Based Cultivation for <i>Pichia pastoris</i> Clone Screening Enables Accelerated and Optimized Recombinant Protein Production Processes
PaperMethanol induction optimization for scFv antibody fragment production in Pichia pastoris.
Cunha, A E, Clemente, J J, Gomes, R, Pinto, F, Thomaz, M, Miranda, S, Pinto, R, Moosmayer, D, Donner, P, Carrondo, M J T · 2004 · Biotechnology and bioengineering · DOI
Full title: Methanol induction optimization for scFv antibody fragment production in Pichia pastoris.
Authors: Cunha, A E, Clemente, J J, Gomes, R, Pinto, F, Thomaz, M, Miranda, S, Pinto, R, Moosmayer, D, Donner, P, Carrondo, M J T
PaperMetabolic modeling to identify engineering targets for <i>Komagataella phaffii</i>: The effect of biomass composition on gene target identification
Cankorur‐Cetinkaya, Ayca, Dikicioǧlu, Duygu, Oliver, Stephen G. · 2017 · Biotechnology and Bioengineering · DOI
Full title: Metabolic modeling to identify engineering targets for <i>Komagataella phaffii</i>: The effect of biomass composition on gene target identification
Authors: Cankorur‐Cetinkaya, Ayca, Dikicioǧlu, Duygu, Oliver, Stephen G.
PaperMethanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors.
Vogl, Thomas, Sturmberger, Lukas, Fauland, Pia C, Hyden, Patrick, Fischer, Jasmin E, Schmid, Christian, Thallinger, Gerhard G, Geier, Martina, Glieder, Anton · 2018 · Biotechnology and bioengineering · DOI
Full title: Methanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors.
Authors: Vogl, Thomas, Sturmberger, Lukas, Fauland, Pia C, Hyden, Patrick, Fischer, Jasmin E, Schmid, Christian, Thallinger, Gerhard G, Geier, Martina, Glieder, Anton
PaperProcess development for the continuous production of heterologous proteins by the industrial yeast, <i>Komagataella phaffii</i>
Cankorur‐Cetinkaya, Ayca, Narraidoo, Nathalie, Kasavi, Ceyda, Slater, Nigel K.H., Archer, David B., Oliver, Stephen G. · 2018 · Biotechnology and Bioengineering · DOI
Full title: Process development for the continuous production of heterologous proteins by the industrial yeast, <i>Komagataella phaffii</i>
Authors: Cankorur‐Cetinkaya, Ayca, Narraidoo, Nathalie, Kasavi, Ceyda, Slater, Nigel K.H., Archer, David B., Oliver, Stephen G.
PaperChallenging the workhorse: Comparative analysis of eukaryotic micro‐organisms for expressing monoclonal antibodies
Jiang, Hanxiao, Horwitz, Andrew A., Wright, Chapman, Tai, Anna, Znameroski, Elizabeth A., Tsegaye, Yoseph, Warbington, Hailley, Bower, Benjamin S., Alves, Christina, Co, Carl, Jonnalagadda, Kanvasri N., Platt, Darren, Walter, Jessica M., Natarajan, Venkatesh, Ubersax, Jeffrey A., Cherry, Joel R., Love, J. Christopher · 2019 · Biotechnology and Bioengineering · DOI
Full title: Challenging the workhorse: Comparative analysis of eukaryotic micro‐organisms for expressing monoclonal antibodies
Authors: Jiang, Hanxiao, Horwitz, Andrew A., Wright, Chapman, Tai, Anna, Znameroski, Elizabeth A., Tsegaye, Yoseph, Warbington, Hailley, Bower, Benjamin S., Alves, Christina, Co, Carl, Jonnalagadda, Kanvasri N., Platt, Darren, Walter, Jessica M., Natarajan, Venkatesh, Ubersax, Jeffrey A., Cherry, Joel R., Love, J. Christopher
PaperEngineering of alcohol dehydrogenase 2 hybrid-promoter architectures in Pichia pastoris to enhance recombinant protein expression on ethanol.
Ergün, Burcu Gündüz, Gasser, Brigitte, Mattanovich, Diethard, Çalık, Pınar · 2019 · Biotechnology and bioengineering · DOI
Full title: Engineering of alcohol dehydrogenase 2 hybrid-promoter architectures in Pichia pastoris to enhance recombinant protein expression on ethanol.
PaperComparative genome‐scale analysis of <i>Pichia pastoris</i> variants informs selection of an optimal base strain
Brady, Joseph R., Whittaker, Charles A., Tan, Melody C., Kristensen, D. Lee, Ma, Duanduan, Dalvie, Neil C., Love, Kerry R., Love, J. Christopher · 2019 · Biotechnology and Bioengineering · DOI
Full title: Comparative genome‐scale analysis of <i>Pichia pastoris</i> variants informs selection of an optimal base strain
Authors: Brady, Joseph R., Whittaker, Charles A., Tan, Melody C., Kristensen, D. Lee, Ma, Duanduan, Dalvie, Neil C., Love, Kerry R., Love, J. Christopher
PaperCharacterization of methanol utilization negative <i>Pichia pastoris</i> for secreted protein production: New cultivation strategies for current and future applications
Zavec, Domen, Gasser, Brigitte, Mattanovich, Diethard · 2020 · Biotechnology and Bioengineering · DOI
Full title: Characterization of methanol utilization negative <i>Pichia pastoris</i> for secreted protein production: New cultivation strategies for current and future applications
PaperModular development enables rapid design of media for alternative hosts
Biedermann, Andrew M., Gengaro, Isabella R., Rodriguez‐Aponte, Sergio A., Love, Kerry R., Love, J. Christopher · 2021 · Biotechnology and Bioengineering · DOI
Full title: Modular development enables rapid design of media for alternative hosts
Authors: Biedermann, Andrew M., Gengaro, Isabella R., Rodriguez‐Aponte, Sergio A., Love, Kerry R., Love, J. Christopher
PaperScalable, methanol-free manufacturing of the SARS-CoV-2 receptor-binding domain in engineered Komagataella phaffii.
Dalvie, Neil C, Biedermann, Andrew M, Rodriguez-Aponte, Sergio A, Naranjo, Christopher A, Rao, Harish D, Rajurkar, Meghraj P, Lothe, Rakesh R, Shaligram, Umesh S, Johnston, Ryan S, Crowell, Laura E, Castelino, Seraphin, Tracey, Mary K, Whittaker, Charles A, Love, J Christopher · 2022 · Biotechnology and bioengineering · DOI
Full title: Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor-binding domain in engineered Komagataella phaffii.
Authors: Dalvie, Neil C, Biedermann, Andrew M, Rodriguez-Aponte, Sergio A, Naranjo, Christopher A, Rao, Harish D, Rajurkar, Meghraj P, Lothe, Rakesh R, Shaligram, Umesh S, Johnston, Ryan S, Crowell, Laura E, Castelino, Seraphin, Tracey, Mary K, Whittaker, Charles A, Love, J Christopher
PaperMinimal purification method enables developability assessment of recombinant proteins
Rodriguez‐Aponte, Sergio A., Naranjo, Christopher A., Johnston, Ryan S., Dalvie, Neil C., Crowell, Laura E., Bajoria, Sakshi, Kumru, Ozan S., Joshi, Sangeeta B., Volkin, David B., Love, J. Christopher · 2023 · Biotechnology and Bioengineering · DOI
Full title: Minimal purification method enables developability assessment of recombinant proteins
Authors: Rodriguez‐Aponte, Sergio A., Naranjo, Christopher A., Johnston, Ryan S., Dalvie, Neil C., Crowell, Laura E., Bajoria, Sakshi, Kumru, Ozan S., Joshi, Sangeeta B., Volkin, David B., Love, J. Christopher
PaperAn ultra scale-down approach to assess the impact of the choice of recombinant P. pastoris strain on dewatering performance in centrifuges.
Lopes, Adriana G, Khan, Nadia, Liddell, John, Keshavarz-Moore, Eli · 2012 · Biotechnology progress · DOI
Full title: An ultra scale-down approach to assess the impact of the choice of recombinant P. pastoris strain on dewatering performance in centrifuges.
Authors: Lopes, Adriana G, Khan, Nadia, Liddell, John, Keshavarz-Moore, Eli
PaperDevelopment of a high-throughput microscale cell disruption platform for Pichia pastoris in rapid bioprocess design.
Bláha, Benjamin A F, Morris, Stephen A, Ogonah, Olotu W, Maucourant, Sophie, Crescente, Vincenzo, Rosenberg, William, Mukhopadhyay, Tarit K · 2018 · Biotechnology progress · DOI
Full title: Development of a high-throughput microscale cell disruption platform for Pichia pastoris in rapid bioprocess design.
Authors: Bláha, Benjamin A F, Morris, Stephen A, Ogonah, Olotu W, Maucourant, Sophie, Crescente, Vincenzo, Rosenberg, William, Mukhopadhyay, Tarit K
PaperBiotransformation of β‐hydroxypyruvate and glycolaldehyde to l ‐erythrulose by <i>Pichia pastoris</i> strain GS115 overexpressing native transketolase
Wei, Yu‐Chia, Braun‐Galleani, Stephanie, Henríquez, María José, Bandara, Sahan, Nesbeth, Darren · 2017 · Biotechnology Progress · DOI
Full title: Biotransformation of β‐hydroxypyruvate and glycolaldehyde to l ‐erythrulose by <i>Pichia pastoris</i> strain GS115 overexpressing native transketolase
Authors: Wei, Yu‐Chia, Braun‐Galleani, Stephanie, Henríquez, María José, Bandara, Sahan, Nesbeth, Darren
PaperHolistic process development to mitigate proteolysis of a subunit rotavirus vaccine candidate produced in Pichia pastoris by means of an acid pH pulse during fed-batch fermentation.
Velez-Suberbie, M Lourdes, Morris, Stephen A, Kaur, Kawaljit, Hickey, John M, Joshi, Sangeeta B, Volkin, David B, Bracewell, Daniel G, Mukhopadhyay, Tarit K · 2020 · Biotechnology progress · DOI
Full title: Holistic process development to mitigate proteolysis of a subunit rotavirus vaccine candidate produced in Pichia pastoris by means of an acid pH pulse during fed-batch fermentation.
Authors: Velez-Suberbie, M Lourdes, Morris, Stephen A, Kaur, Kawaljit, Hickey, John M, Joshi, Sangeeta B, Volkin, David B, Bracewell, Daniel G, Mukhopadhyay, Tarit K
PaperDesign of a novel automated methanol feed system for pilot-scale fermentation of Pichia pastoris.
Hamaker, Kent H, Johnson, Daniel C, Bellucci, Joseph J, Apgar, Kristie R, Soslow, Sherry, Gercke, John C, Menzo, Darrin J, Ton, Christopher · 2011 · Biotechnology progress · DOI
Full title: Design of a novel automated methanol feed system for pilot-scale fermentation of Pichia pastoris.
Authors: Hamaker, Kent H, Johnson, Daniel C, Bellucci, Joseph J, Apgar, Kristie R, Soslow, Sherry, Gercke, John C, Menzo, Darrin J, Ton, Christopher
PaperHigh level expression, purification, and characterization of the Kunitz-type protease inhibitor domain of protease nexin-2/amyloid beta-protein precursor.
Wagner, S L, Siegel, R S, Vedvick, T S, Raschke, W C, Van Nostrand, W E · 1992 · Biochemical and biophysical research communications · DOI
Full title: High level expression, purification, and characterization of the Kunitz-type protease inhibitor domain of protease nexin-2/amyloid beta-protein precursor.
Authors: Wagner, S L, Siegel, R S, Vedvick, T S, Raschke, W C, Van Nostrand, W E
Year: 1992
Venue: Biochemical and biophysical research communications
PaperTransfer of disulfide bond formation modules via yeast artificial chromosomes promotes the expression of heterologous proteins in Kluyveromyces marxianus.
Full title: Transfer of disulfide bond formation modules via yeast artificial chromosomes promotes the expression of heterologous proteins in Kluyveromyces marxianus.
PaperProduction of functionalized single-chain Fv antibody fragments binding to the ED-B domain of the B-isoform of fibronectin in Pichia pastoris.
Marty, C, Scheidegger, P, Ballmer-Hofer, K, Klemenz, R, Schwendener, R A · 2001 · Protein expression and purification · DOI
Full title: Production of functionalized single-chain Fv antibody fragments binding to the ED-B domain of the B-isoform of fibronectin in Pichia pastoris.
Authors: Marty, C, Scheidegger, P, Ballmer-Hofer, K, Klemenz, R, Schwendener, R A
PaperIncreasing the cell viability and heterologous protein expression of Pichia pastoris mutant deficient in PMR1 gene by culture condition optimization.
Zhao, Hong Liang, Xue, Chong, Wang, Yang, Yao, Xue Qin, Liu, Zhi Min · 2008 · Applied microbiology and biotechnology · DOI
Full title: Increasing the cell viability and heterologous protein expression of Pichia pastoris mutant deficient in PMR1 gene by culture condition optimization.
Authors: Zhao, Hong Liang, Xue, Chong, Wang, Yang, Yao, Xue Qin, Liu, Zhi Min
PaperConstitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris.
Menéndez, Carmen, Martínez, Duniesky, Trujillo, Luis E, Mazola, Yuliet, González, Ernesto, Pérez, Enrique R, Hernández, Lázaro · 2013 · Applied microbiology and biotechnology · DOI
Full title: Constitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris.
Authors: Menéndez, Carmen, Martínez, Duniesky, Trujillo, Luis E, Mazola, Yuliet, González, Ernesto, Pérez, Enrique R, Hernández, Lázaro
PaperOptimization of the pilot-scale production of an ice-binding protein by fed-batch culture of Pichia pastoris.
Lee, Jun Hyuck, Lee, Sung Gu, Do, Hackwon, Park, Jong Chan, Kim, Eunjung, Choe, Yong-Hoe, Han, Se Jong, Kim, Hak Jun · 2013 · Applied microbiology and biotechnology · DOI
Full title: Optimization of the pilot-scale production of an ice-binding protein by fed-batch culture of Pichia pastoris.
Authors: Lee, Jun Hyuck, Lee, Sung Gu, Do, Hackwon, Park, Jong Chan, Kim, Eunjung, Choe, Yong-Hoe, Han, Se Jong, Kim, Hak Jun
PaperHigh expression of a plectasin-derived peptide NZ2114 in Pichia pastoris and its pharmacodynamics, postantibiotic and synergy against Staphylococcus aureus.
Zhang, Yong, Teng, Da, Mao, Ruoyu, Wang, Xiumin, Xi, Di, Hu, Xiaoyuan, Wang, Jianhua · 2014 · Applied microbiology and biotechnology · DOI
Full title: High expression of a plectasin-derived peptide NZ2114 in Pichia pastoris and its pharmacodynamics, postantibiotic and synergy against Staphylococcus aureus.
PaperSecretion and assembly of calicivirus-like particles in high-cell-density yeast fermentations: strategies based on a recombinant non-specific BPTI-Kunitz-type protease inhibitor.
Full title: Secretion and assembly of calicivirus-like particles in high-cell-density yeast fermentations: strategies based on a recombinant non-specific BPTI-Kunitz-type protease inhibitor.
PaperIn vitro and in vivo characterization of a new recombinant antimicrobial peptide, MP1102, against methicillin-resistant Staphylococcus aureus.
Zhang, Yong, Teng, Da, Wang, Xiumin, Mao, Ruoyu, Cao, Xintao, Hu, Xiaoyuan, Zong, Lifen, Wang, Jianhua · 2015 · Applied microbiology and biotechnology · DOI
Full title: In vitro and in vivo characterization of a new recombinant antimicrobial peptide, MP1102, against methicillin-resistant Staphylococcus aureus.
PaperImproving heterologous expression of porcine follicle-stimulating hormone in Pichia pastoris by integrating molecular strategies and culture condition optimization.
Luo, Gang, Tian, JianHui, Huang, Huoqing, Lei An · 2018 · Applied microbiology and biotechnology · DOI
Full title: Improving heterologous expression of porcine follicle-stimulating hormone in Pichia pastoris by integrating molecular strategies and culture condition optimization.
Authors: Luo, Gang, Tian, JianHui, Huang, Huoqing, Lei An
PaperIs heterogeneity in large-scale bioreactors a real problem in recombinant protein synthesis by Pichia pastoris?
Edgar Velastegui, J. Quezada, Karlo Guerrero, C. Altamirano, Juan A. Martínez, J. Berrios, P. Fickers · 2023 · Applied Microbiology and Biotechnology · DOI
Full title: Is heterogeneity in large-scale bioreactors a real problem in recombinant protein synthesis by Pichia pastoris?
Authors: Edgar Velastegui, J. Quezada, Karlo Guerrero, C. Altamirano, Juan A. Martínez, J. Berrios, P. Fickers
PaperA simple Pichia pastoris fermentation and downstream processing strategy for making recombinant pandemic Swine Origin Influenza a virus Hemagglutinin protein.
Athmaram, T N, Singh, Anil Kumar, Saraswat, Shweta, Srivastava, Saurabh, Misra, Princi, Kameswara Rao, M, Gopalan, N, Rao, P V L · 2013 · Journal of industrial microbiology & biotechnology · DOI
Full title: A simple Pichia pastoris fermentation and downstream processing strategy for making recombinant pandemic Swine Origin Influenza a virus Hemagglutinin protein.
Authors: Athmaram, T N, Singh, Anil Kumar, Saraswat, Shweta, Srivastava, Saurabh, Misra, Princi, Kameswara Rao, M, Gopalan, N, Rao, P V L
Year: 2013
Venue: Journal of industrial microbiology & biotechnology
PaperInfluence of methanol/sorbitol co-feeding rate on pAOX1 induction in a Pichia pastoris Mut+ strain in bioreactor with limited oxygen transfer rate.
Carly, F, Niu, H, Delvigne, F, Fickers, P · 2016 · Journal of industrial microbiology & biotechnology · DOI
Full title: Influence of methanol/sorbitol co-feeding rate on pAOX1 induction in a Pichia pastoris Mut+ strain in bioreactor with limited oxygen transfer rate.
Authors: Carly, F, Niu, H, Delvigne, F, Fickers, P
Year: 2016
Venue: Journal of industrial microbiology & biotechnology
PaperA comparative study of glycerol and sorbitol as co-substrates in methanol-induced cultures of Pichia pastoris: temperature effect and scale-up simulation.
Full title: A comparative study of glycerol and sorbitol as co-substrates in methanol-induced cultures of Pichia pastoris: temperature effect and scale-up simulation.
PaperRapid screening of cellular stress responses in recombinant <i>Pichia pastoris</i> strains using metabolite profiling
Tredwell, Gregory D., Aw, Rochelle, Edwards-Jones, Bryn, Leak, David J., Bundy, Jacob G. · 2017 · Journal of Industrial Microbiology & Biotechnology · DOI
Full title: Rapid screening of cellular stress responses in recombinant <i>Pichia pastoris</i> strains using metabolite profiling
Authors: Tredwell, Gregory D., Aw, Rochelle, Edwards-Jones, Bryn, Leak, David J., Bundy, Jacob G.
Year: 2017
Venue: Journal of Industrial Microbiology & Biotechnology
PaperKinetic studies of constitutive human granulocyte-macrophage colony stimulating factor (hGM-CSF) expression in continuous culture of Pichia pastoris.
Khasa, Yogender Pal, Khushoo, Amardeep, Srivastava, Leena, Mukherjee, K J · 2007 · Biotechnology letters · DOI
Full title: Kinetic studies of constitutive human granulocyte-macrophage colony stimulating factor (hGM-CSF) expression in continuous culture of Pichia pastoris.
PaperEnhancing pIFN-α production and process stability in fed-batch culture of Pichia pastoris by controlling the methanol concentration and monitoring the responses of OUR/DO levels.
Full title: Enhancing pIFN-α production and process stability in fed-batch culture of Pichia pastoris by controlling the methanol concentration and monitoring the responses of OUR/DO levels.
PaperIncrease of Candida antarctica lipase B production under PGK promoter in Pichia pastoris: effect of multicopies
Robert, Julia de Macedo, Betancur, Maritza Ocampo, Machado, Antonio Carlos Oliveira, Arruda, Andrelisse, Reis, Viviane Castelo Branco, Almeida, Rodrigo Volcan, Torres, Fernando Araripe Gonçalves, Alegre, Pau Ferrer i, Valero, Francisco, Freire, Denise Maria Guimarães · 2019 · Brazilian Journal of Microbiology · DOI
Full title: Increase of Candida antarctica lipase B production under PGK promoter in Pichia pastoris: effect of multicopies
Authors: Robert, Julia de Macedo, Betancur, Maritza Ocampo, Machado, Antonio Carlos Oliveira, Arruda, Andrelisse, Reis, Viviane Castelo Branco, Almeida, Rodrigo Volcan, Torres, Fernando Araripe Gonçalves, Alegre, Pau Ferrer i, Valero, Francisco, Freire, Denise Maria Guimarães
PaperSimultaneously promoting gellan lyase expression and gellan oligosaccharide production by gellan gum co-feeding during Pichia pastoris fermentation
Tong Liang, Yuying Wang, Feiyang Duan, Yaping Pan, Zepeng Chang, Qijun Pan, Yun Jiang, Zhitao Li, Minjie Gao · 2025 · Systems Microbiology and Biomanufacturing · DOI
Full title: Simultaneously promoting gellan lyase expression and gellan oligosaccharide production by gellan gum co-feeding during Pichia pastoris fermentation
PaperCharacterization of wheat germin (oxalate oxidase) expressed by Pichia pastoris.
Pan, Heng-Yen, Whittaker, Mei M, Bouveret, Romaric, Berna, Anne, Bernier, François, Whittaker, James W · 2007 · Biochemical and biophysical research communications · DOI
Full title: Characterization of wheat germin (oxalate oxidase) expressed by Pichia pastoris.
Authors: Pan, Heng-Yen, Whittaker, Mei M, Bouveret, Romaric, Berna, Anne, Bernier, François, Whittaker, James W
Year: 2007
Venue: Biochemical and biophysical research communications
PaperBioproduced Proteins On Demand (Bio-POD) in hydrogels using Pichia pastoris.
Yuan, Shuo-Fu, Brooks, Sierra M, Nguyen, Annalee W, Lin, Wen-Ling, Johnston, Trevor G, Maynard, Jennifer A, Nelson, Alshakim, Alper, Hal S · 2021 · Bioactive materials · DOI
Full title: Bioproduced Proteins On Demand (Bio-POD) in hydrogels using Pichia pastoris.
Authors: Yuan, Shuo-Fu, Brooks, Sierra M, Nguyen, Annalee W, Lin, Wen-Ling, Johnston, Trevor G, Maynard, Jennifer A, Nelson, Alshakim, Alper, Hal S
PaperHigh-yield secretory production of stable, active trypsin through engineering of the N-terminal peptide and self-degradation sites in Pichia pastoris.
Zhang, Yunfeng, Huang, Hao, Yao, Xinhui, Du, Guocheng, Chen, Jian, Kang, Zhen · 2018 · Bioresource technology · DOI
Full title: High-yield secretory production of stable, active trypsin through engineering of the N-terminal peptide and self-degradation sites in Pichia pastoris.
PaperConstruction of an efficient enzyme-cell@material biocatalyst through the biofilm immobilization of Komagataella phaffii for continuous biocatalysis.
Full title: Construction of an efficient enzyme-cell@material biocatalyst through the biofilm immobilization of Komagataella phaffii for continuous biocatalysis.
PaperCarbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast.
Zepeda, Andrea B, Pessoa, Adalberto, Farías, Jorge G · 2018 · Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · DOI
Full title: Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast.
Authors: Zepeda, Andrea B, Pessoa, Adalberto, Farías, Jorge G
Year: 2018
Venue: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
PaperDesigning and developing a sensitive and specific SARS-CoV-2 RBD IgG detection kit for identifying positive human samples.
Raoufi, Ehsan, Hosseini, Fatemeh, Onagh, Bahman, Salehi-Shadkami, Mohammad, Mehrali, Marjan, Mohsenzadegan, Monireh, Ho, Jim Q, Bigdelou, Banafsheh, Sepand, Mohammad Reza, Webster, Thomas J, Zanganeh, Steven, Farajollahi, Mohammad M · 2023 · Clinica chimica acta; international journal of clinical chemistry · DOI
Full title: Designing and developing a sensitive and specific SARS-CoV-2 RBD IgG detection kit for identifying positive human samples.
Authors: Raoufi, Ehsan, Hosseini, Fatemeh, Onagh, Bahman, Salehi-Shadkami, Mohammad, Mehrali, Marjan, Mohsenzadegan, Monireh, Ho, Jim Q, Bigdelou, Banafsheh, Sepand, Mohammad Reza, Webster, Thomas J, Zanganeh, Steven, Farajollahi, Mohammad M
Year: 2023
Venue: Clinica chimica acta; international journal of clinical chemistry
PaperHeterologous expression and biochemical characterization of a novel thermostable Sclerotinia sclerotiorum GH45 endoglucanase in Pichia pastoris.
Chahed, Haifa, Boumaiza, Mohamed, Ezzine, Aymen, Marzouki, M Nejib · 2018 · International journal of biological macromolecules · DOI
Full title: Heterologous expression and biochemical characterization of a novel thermostable Sclerotinia sclerotiorum GH45 endoglucanase in Pichia pastoris.
Authors: Chahed, Haifa, Boumaiza, Mohamed, Ezzine, Aymen, Marzouki, M Nejib
Year: 2018
Venue: International journal of biological macromolecules
PaperCombined effect of gene dosage and process optimization strategies on high-level production of recombinant human interleukin-3 (hIL-3) in Pichia pastoris fed-batch culture.
Dagar, Vikas Kumar, Khasa, Yogender Pal · 2018 · International journal of biological macromolecules · DOI
Full title: Combined effect of gene dosage and process optimization strategies on high-level production of recombinant human interleukin-3 (hIL-3) in Pichia pastoris fed-batch culture.
Authors: Dagar, Vikas Kumar, Khasa, Yogender Pal
Year: 2018
Venue: International journal of biological macromolecules
PaperAssessing virus like particles formation and r-HBsAg aggregation during large scale production of recombinant hepatitis B surface antigen from Pichia pastoris.
Hosseini, Seyed Nezamedin, Sarvari, Taravat, Bashiri, Ghazal, Khatami, Maryam, Shojaosadati, Seyed Abbas · 2019 · International journal of biological macromolecules · DOI
Full title: Assessing virus like particles formation and r-HBsAg aggregation during large scale production of recombinant hepatitis B surface antigen from Pichia pastoris.
PaperCodon-optimized production of Cu/Zn superoxide dismutase (SOD1) in Komagataella phaffii: Functional characterization and high-yield fermentation strategy.
Al-Adeeb, Abdulqader, Aqeel, Sahibzada Muhammad, Gu, Qiuya, Ren, Qiuhuan, Liu, Wen-Long, Mahdi, Amer Ali, Yu, Xiaobin · 2025 · International journal of biological macromolecules · DOI
Full title: Codon-optimized production of Cu/Zn superoxide dismutase (SOD1) in Komagataella phaffii: Functional characterization and high-yield fermentation strategy.
PaperInvestigation of the physiological response to oxygen limited process conditions of Pichia pastoris Mut(+) strain using a two-compartment scale-down system.
Lorantfy, Bettina, Jazini, Mohammadhadi, Herwig, Christoph · 2013 · Journal of bioscience and bioengineering · DOI
Full title: Investigation of the physiological response to oxygen limited process conditions of Pichia pastoris Mut(+) strain using a two-compartment scale-down system.
Authors: Lorantfy, Bettina, Jazini, Mohammadhadi, Herwig, Christoph
PaperAdaptive dissolved oxygen control through the glycerol feeding in a recombinant Pichia pastoris cultivation in conditions of oxygen transfer limitation.
Oliveira, R, Clemente, J J, Cunha, A E, Carrondo, M J T · 2005 · Journal of biotechnology · DOI
Full title: Adaptive dissolved oxygen control through the glycerol feeding in a recombinant Pichia pastoris cultivation in conditions of oxygen transfer limitation.
Authors: Oliveira, R, Clemente, J J, Cunha, A E, Carrondo, M J T
PaperA synergistic effect on the production of S-adenosyl-L-methionine in Pichia pastoris by knocking in of S-adenosyl-L-methionine synthase and knocking out of cystathionine-beta synthase.
He, Junyun, Deng, Juanjuan, Zheng, Yinghua, Gu, Jun · 2006 · Journal of biotechnology · DOI
Full title: A synergistic effect on the production of S-adenosyl-L-methionine in Pichia pastoris by knocking in of S-adenosyl-L-methionine synthase and knocking out of cystathionine-beta synthase.
Authors: He, Junyun, Deng, Juanjuan, Zheng, Yinghua, Gu, Jun
PaperEffects of different glycerol feeding strategies on S-adenosyl-l-methionine biosynthesis by P(GAP)-driven Pichia pastoris overexpressing methionine adenosyltransferase.
Full title: Effects of different glycerol feeding strategies on S-adenosyl-l-methionine biosynthesis by P(GAP)-driven Pichia pastoris overexpressing methionine adenosyltransferase.
PaperExpression of coxsackievirus and adenovirus receptor (CAR)-Fc fusion protein in Pichia pastoris and characterization of its anti-coxsackievirus activity.
Full title: Expression of coxsackievirus and adenovirus receptor (CAR)-Fc fusion protein in Pichia pastoris and characterization of its anti-coxsackievirus activity.
PaperDe-glycosylation of Pichia pastoris-produced Schistosoma mansoni cathepsin B eliminates non-specific reactivity with IgG in normal human serum.
Stack, Colin M, Dalton, John P, Cunneen, Mary, Donnelly, Sheila · 2005 · Journal of immunological methods · DOI
Full title: De-glycosylation of Pichia pastoris-produced Schistosoma mansoni cathepsin B eliminates non-specific reactivity with IgG in normal human serum.
Authors: Stack, Colin M, Dalton, John P, Cunneen, Mary, Donnelly, Sheila
PaperRational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagataella phaffii) using the methanol-free GAP promoter. Where do we stand?
Garcia‐Ortega, Xavier, Cámara, Elena, Ferrer, Pau, Albiol, Joan, Montesinos, José Luis González, Valero, Francisco · 2019 · New Biotechnology · DOI
Full title: Rational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagataella phaffii) using the methanol-free GAP promoter. Where do we stand?
Authors: Garcia‐Ortega, Xavier, Cámara, Elena, Ferrer, Pau, Albiol, Joan, Montesinos, José Luis González, Valero, Francisco
PaperScale-up of the fermentation and purification of the recombinant heavy chain fragment C of botulinum neurotoxin serotype F, expressed in Pichia pastoris.
Johnson, Scott K, Zhang, Wenhui, Smith, Leonard A, Hywood-Potter, Karen J, Todd Swanson, S, Schlegel, Vicki L, Meagher, Michael M · 2003 · Protein expression and purification · DOI
Full title: Scale-up of the fermentation and purification of the recombinant heavy chain fragment C of botulinum neurotoxin serotype F, expressed in Pichia pastoris.
Authors: Johnson, Scott K, Zhang, Wenhui, Smith, Leonard A, Hywood-Potter, Karen J, Todd Swanson, S, Schlegel, Vicki L, Meagher, Michael M
PaperPurification and scale-up of a recombinant heavy chain fragment C of botulinum neurotoxin serotype E in Pichia pastoris GS115.
Dux, Michael P, Barent, Rick, Sinha, Jayanta, Gouthro, Mark, Swanson, Todd, Barthuli, Ardis, Inan, Mehmet, Ross, John T, Smith, Leonard A, Smith, Theresa J, Webb, Robert, Loveless, Bonnie, Henderson, Ian, Meagher, Michael M · 2006 · Protein expression and purification · DOI
Full title: Purification and scale-up of a recombinant heavy chain fragment C of botulinum neurotoxin serotype E in Pichia pastoris GS115.
Authors: Dux, Michael P, Barent, Rick, Sinha, Jayanta, Gouthro, Mark, Swanson, Todd, Barthuli, Ardis, Inan, Mehmet, Ross, John T, Smith, Leonard A, Smith, Theresa J, Webb, Robert, Loveless, Bonnie, Henderson, Ian, Meagher, Michael M
PaperAn acidic and thermostable carboxymethyl cellulase from the yeast Cryptococcus sp. S-2: purification, characterization and improvement of its recombinant enzyme production by high cell-density fermentation of Pichia pastoris.
Thongekkaew, Jantaporn, Ikeda, Hiroko, Masaki, Kazuo, Iefuji, Haruyuki · 2008 · Protein expression and purification · DOI
Full title: An acidic and thermostable carboxymethyl cellulase from the yeast Cryptococcus sp. S-2: purification, characterization and improvement of its recombinant enzyme production by high cell-density fermentation of Pichia pastoris.
PaperExpression, purification, and molecular analysis of the Necator americanus glutathione S-transferase 1 (Na-GST-1): a production process developed for a lead candidate recombinant hookworm vaccine antigen.
Goud, Gaddam Narsa, Deumic, Vehid, Gupta, Richi, Brelsford, Jill, Zhan, Bin, Gillespie, Portia, Plieskatt, Jordan L, Tsao, Eric I, Hotez, Peter J, Bottazzi, Maria Elena · 2012 · Protein expression and purification · DOI
Full title: Expression, purification, and molecular analysis of the Necator americanus glutathione S-transferase 1 (Na-GST-1): a production process developed for a lead candidate recombinant hookworm vaccine antigen.
Authors: Goud, Gaddam Narsa, Deumic, Vehid, Gupta, Richi, Brelsford, Jill, Zhan, Bin, Gillespie, Portia, Plieskatt, Jordan L, Tsao, Eric I, Hotez, Peter J, Bottazzi, Maria Elena
PaperHigh-level extracellular production of Rhizopus oryzae lipase in Pichia pastoris via a strategy combining optimization of gene-copy number with co-expression of ERAD-related proteins.
Jiao, Liangcheng, Zhou, Qinghua, Su, Zhixin, Xu, Li, Yan, Yunjun · 2018 · Protein expression and purification · DOI
Full title: High-level extracellular production of Rhizopus oryzae lipase in Pichia pastoris via a strategy combining optimization of gene-copy number with co-expression of ERAD-related proteins.
PaperEfficient production of a recombinant Venerupis philippinarum defensin (VpDef) in Pichia pastoris and characterization of its antibacterial activity and stability.
Meng, De-Mei, Lv, Yu-Jie, Zhao, Jing-Fang, Liu, Qing-Yan, Shi, Lin-Yue, Wang, Jun-Ping, Yang, Yong-Hai, Fan, Zhen-Chuan · 2018 · Protein expression and purification · DOI
Full title: Efficient production of a recombinant Venerupis philippinarum defensin (VpDef) in Pichia pastoris and characterization of its antibacterial activity and stability.
PaperHighly secretory expression of recombinant cowpea chlorotic mottle virus capsid proteins in Pichia pastoris and in-vitro encapsulation of ruthenium nanoparticles for catalysis.
Zhu, Jie, Yang, Kun, Liu, Aijie, Lu, Xiaoxue, Yang, Linsong, Zhao, Qinghuan · 2020 · Protein expression and purification · DOI
Full title: Highly secretory expression of recombinant cowpea chlorotic mottle virus capsid proteins in Pichia pastoris and in-vitro encapsulation of ruthenium nanoparticles for catalysis.
PaperProduction, quality control, stability and pharmacotoxicity of cGMP-produced Plasmodium falciparum AMA1 FVO strain ectodomain expressed in Pichia pastoris.
Faber, Bart W, Remarque, Edmond J, Kocken, Clemens H M, Cheront, Pascale, Cingolani, Doriano, Xhonneux, Florence, Jurado, Margarita, Haumont, Michele, Jepsen, Soren, Leroy, Odile, Thomas, Alan W · 2008 · Vaccine · DOI
Full title: Production, quality control, stability and pharmacotoxicity of cGMP-produced Plasmodium falciparum AMA1 FVO strain ectodomain expressed in Pichia pastoris.
Authors: Faber, Bart W, Remarque, Edmond J, Kocken, Clemens H M, Cheront, Pascale, Cingolani, Doriano, Xhonneux, Florence, Jurado, Margarita, Haumont, Michele, Jepsen, Soren, Leroy, Odile, Thomas, Alan W
Full title: Recombinant gp90 protein expressed in Pichia pastoris induces a protective immune response against reticuloendotheliosis virus in chickens.
PaperCOVID-19 management landscape: A need for an affordable platform to manufacture safe and efficacious biotherapeutics and prophylactics for the developing countries.
Full title: COVID-19 management landscape: A need for an affordable platform to manufacture safe and efficacious biotherapeutics and prophylactics for the developing countries.
PaperInitial purification of recombinant botulinum neurotoxin fragments for pharmaceutical production using hydrophobic charge induction chromatography.
Weatherly, Gresham T, Bouvier, Anne, Lydiard, Debra D, Chapline, Jaymi, Henderson, Ian, Schrimsher, Jeffrey L, Shepard, Scot R · 2002 · Journal of chromatography. A · DOI
Full title: Initial purification of recombinant botulinum neurotoxin fragments for pharmaceutical production using hydrophobic charge induction chromatography.
PaperA mini-scale mass production and separation system for secretory heterologous proteins by perfusion culture of recombinant Pichia pastoris using a shaken ceramic membrane flask.
Ohashi, R, Mochizuki, E, Suzuki, T · 1999 · Journal of bioscience and bioengineering · DOI
Full title: A mini-scale mass production and separation system for secretory heterologous proteins by perfusion culture of recombinant Pichia pastoris using a shaken ceramic membrane flask.
PaperHuman chymotrypsinogen B production from Pichia pastoris by integrated development of fermentation and downstream processing. Part 2. Protein recovery.
Thömmes, J, Halfar, M, Gieren, H, Curvers, S, Takors, R, Brunschier, R, Kula, M R · 2001 · Biotechnology progress · DOI
Full title: Human chymotrypsinogen B production from Pichia pastoris by integrated development of fermentation and downstream processing. Part 2. Protein recovery.
Authors: Thömmes, J, Halfar, M, Gieren, H, Curvers, S, Takors, R, Brunschier, R, Kula, M R
PaperCodon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase.
Chang, Shu-Wei, Lee, Guan-Chiun, Shaw, Jei-Fu · 2006 · Journal of agricultural and food chemistry · DOI
Full title: Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase.
PaperProduction and biochemical characterization of the recombinant Boophilus microplus Bm95 antigen from Pichia pastoris.
Boué, O, Farnós, O, González, A, Fernández, R, Acosta, J A, Valdés, R, González, L J, Guanche, Y, Izquierdo, G, Suárez, M, Domínguez, I, Machado, H, Rodríguez, M, Lleonart, R · 2004 · Experimental & applied acarology · DOI
Full title: Production and biochemical characterization of the recombinant Boophilus microplus Bm95 antigen from Pichia pastoris.
Authors: Boué, O, Farnós, O, González, A, Fernández, R, Acosta, J A, Valdés, R, González, L J, Guanche, Y, Izquierdo, G, Suárez, M, Domínguez, I, Machado, H, Rodríguez, M, Lleonart, R
PaperHigh-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris.
Laroche, Y, Storme, V, De Meutter, J, Messens, J, Lauwereys, M · 1994 · Bio/technology (Nature Publishing Company) · DOI
Full title: High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Pichia pastoris.
Authors: Laroche, Y, Storme, V, De Meutter, J, Messens, J, Lauwereys, M
PaperEngineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice
Dalvie, Neil C., Rodriguez‐Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Célia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dong Soo, Brunette, Natalie, Wang, Jing Yang, Walkey, Carl, Fiala, Brooke, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanné, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith M., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice, Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher · 2021 · Proceedings of the National Academy of Sciences · DOI
Full title: Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice
Authors: Dalvie, Neil C., Rodriguez‐Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Célia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dong Soo, Brunette, Natalie, Wang, Jing Yang, Walkey, Carl, Fiala, Brooke, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanné, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith M., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice, Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher
Year: 2021
Venue: Proceedings of the National Academy of Sciences
PaperLarge scale purification of detergent-soluble P-glycoprotein from Pichia pastoris cells and characterization of nucleotide binding properties of wild-type, Walker A, and Walker B mutant proteins.
Lerner-Marmarosh, N, Gimi, K, Urbatsch, I L, Gros, P, Senior, A E · 1999 · The Journal of biological chemistry · DOI
Full title: Large scale purification of detergent-soluble P-glycoprotein from Pichia pastoris cells and characterization of nucleotide binding properties of wild-type, Walker A, and Walker B mutant proteins.
Authors: Lerner-Marmarosh, N, Gimi, K, Urbatsch, I L, Gros, P, Senior, A E
PaperEnhancement of recombinant BmK AngM1 production in Pichia pastoris by regulating gene dosage, co-expressing with chaperones and fermenting in fed-batch mode.
Wang, Qing-Hua, Liang, Lan, Liu, Wan-Cang, Gong, Ting, Chen, Jing-Jing, Hou, Qi, Yang, Jin-Ling, Zhu, Ping · 2017 · Journal of Asian natural products research · DOI
Full title: Enhancement of recombinant BmK AngM1 production in Pichia pastoris by regulating gene dosage, co-expressing with chaperones and fermenting in fed-batch mode.
PaperLarge-scale purification of recombinant hepatitis B surface antigen from Pichia pastoris with non-affinity chromatographic methods as a substitute to immunoaffinity chromatography.
Full title: Large-scale purification of recombinant hepatitis B surface antigen from Pichia pastoris with non-affinity chromatographic methods as a substitute to immunoaffinity chromatography.
PaperDextranase production by recombinant Pichia pastoris under operational volumetric mass transfer coefficient (kLa) and volumetric gassed power input (Pg/V) attainable at commercial large scale.
Full title: Dextranase production by recombinant Pichia pastoris under operational volumetric mass transfer coefficient (kLa) and volumetric gassed power input (Pg/V) attainable at commercial large scale.
PaperExpression and purification of an engineered, yeast-expressed Leishmania donovani nucleoside hydrolase with immunogenic properties.
Hudspeth, Elissa M, Wang, Qian, Seid, Christopher A, Hammond, Molly, Wei, Junfei, Liu, Zhuyun, Zhan, Bin, Pollet, Jeroen, Heffernan, Michael J, McAtee, C Patrick, Engler, David A, Matsunami, Risë K, Strych, Ulrich, Asojo, Oluwatoyin A, Hotez, Peter J, Bottazzi, Maria Elena · 2016 · Human vaccines & immunotherapeutics · DOI
Full title: Expression and purification of an engineered, yeast-expressed Leishmania donovani nucleoside hydrolase with immunogenic properties.
Authors: Hudspeth, Elissa M, Wang, Qian, Seid, Christopher A, Hammond, Molly, Wei, Junfei, Liu, Zhuyun, Zhan, Bin, Pollet, Jeroen, Heffernan, Michael J, McAtee, C Patrick, Engler, David A, Matsunami, Risë K, Strych, Ulrich, Asojo, Oluwatoyin A, Hotez, Peter J, Bottazzi, Maria Elena
PaperHigh yield expression and secretion of the ovine pregnancy recognition hormone interferon-tau by Pichia pastoris.
Van Heeke, G, Ott, T L, Strauss, A, Ammaturo, D, Bazer, F W · 1996 · Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · DOI
Full title: High yield expression and secretion of the ovine pregnancy recognition hormone interferon-tau by Pichia pastoris.
Authors: Van Heeke, G, Ott, T L, Strauss, A, Ammaturo, D, Bazer, F W
Year: 1996
Venue: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research
PaperRecombinant protein production in <i>Pichia pastoris</i>: from transcriptionally redesigned strains to bioprocess optimization and metabolic modelling
Ergün, Burcu Gündüz, Berríos, Julio, Bi̇nay, Barış, Fickers, Patrick · 2021 · FEMS Yeast Research · DOI
Full title: Recombinant protein production in <i>Pichia pastoris</i>: from transcriptionally redesigned strains to bioprocess optimization and metabolic modelling
Authors: Ergün, Burcu Gündüz, Berríos, Julio, Bi̇nay, Barış, Fickers, Patrick
PaperEngineering the expression system for <i>Komagataella phaffii (Pichia pastoris)</i>: an attempt to develop a methanol-free expression system
Takagi, Shinobu, Tsutsumi, Noriko, Terui, Yuji, Kong, Xiangyu, Yurimoto, Hiroya, Sakai, Yasuyoshi · 2019 · FEMS Yeast Research · DOI
Full title: Engineering the expression system for <i>Komagataella phaffii (Pichia pastoris)</i>: an attempt to develop a methanol-free expression system
PaperEngineering a soluble extracellular erythropoietin receptor (EPObp) in Pichia pastoris to eliminate microheterogeneity, and its complex with erythropoietin.
Zhan, H, Liu, B, Reid, S W, Aoki, K H, Li, C, Syed, R S, Karkaria, C, Koe, G, Sitney, K, Hayenga, K, Mistry, F, Savel, L, Dreyer, M, Katz, B A, Schreurs, J, Matthews, D J, Cheetham, J C, Egrie, J, Giebel, L B, Stroud, R M · 1999 · Protein engineering · DOI
Full title: Engineering a soluble extracellular erythropoietin receptor (EPObp) in Pichia pastoris to eliminate microheterogeneity, and its complex with erythropoietin.
Authors: Zhan, H, Liu, B, Reid, S W, Aoki, K H, Li, C, Syed, R S, Karkaria, C, Koe, G, Sitney, K, Hayenga, K, Mistry, F, Savel, L, Dreyer, M, Katz, B A, Schreurs, J, Matthews, D J, Cheetham, J C, Egrie, J, Giebel, L B, Stroud, R M
PaperLarge-scale expression, purification and characterization of small fragments of thrombomodulin: the roles of the sixth domain and of methionine 388.
White, C E, Hunter, M J, Meininger, D P, White, L R, Komives, E A · 1995 · Protein engineering · DOI
Full title: Large-scale expression, purification and characterization of small fragments of thrombomodulin: the roles of the sixth domain and of methionine 388.
Authors: White, C E, Hunter, M J, Meininger, D P, White, L R, Komives, E A
PaperSecretory expression of glycosylated and aglycosylated mutein of onconase from Pichia pastoris using different secretion signals and their purification and characterization.
Zhao, Hong Liang, He, Qing, Xue, Chong, Sun, Bo, Yao, Xue Qin, Liu, Zhi Min · 2009 · FEMS yeast research · DOI
Full title: Secretory expression of glycosylated and aglycosylated mutein of onconase from Pichia pastoris using different secretion signals and their purification and characterization.
Authors: Zhao, Hong Liang, He, Qing, Xue, Chong, Sun, Bo, Yao, Xue Qin, Liu, Zhi Min
PaperHeterologous protein expression in Pichia thermomethanolica BCC16875, a thermotolerant methylotrophic yeast and characterization of N-linked glycosylation in secreted protein.
Full title: Heterologous protein expression in Pichia thermomethanolica BCC16875, a thermotolerant methylotrophic yeast and characterization of N-linked glycosylation in secreted protein.
PaperEvaluación de la enzima alfa-N-acetilglucosaminidasa humana recombinante obtenida en Komagataella phaffii GS115 en un modelo in vitro de fibroblastos MPS IIIB
Full title: Evaluación de la enzima alfa-N-acetilglucosaminidasa humana recombinante obtenida en Komagataella phaffii GS115 en un modelo in vitro de fibroblastos MPS IIIB
PaperFunctional Expression of Two Unusual Acidic Peroxygenases from Candolleomyces aberdarensis in Yeasts by Adopting Evolved Secretion Mutations.
Gomez de Santos, Patricia, Hoang, Manh Dat, Kiebist, Jan, Kellner, Harald, Ullrich, René, Scheibner, Katrin, Hofrichter, Martin, Liers, Christiane, Alcalde, Miguel · 2021 · Applied and environmental microbiology · DOI
Full title: Functional Expression of Two Unusual Acidic Peroxygenases from Candolleomyces aberdarensis in Yeasts by Adopting Evolved Secretion Mutations.
PaperHigh-level expression of the malaria blood-stage vaccine candidate Plasmodium falciparum apical membrane antigen 1 and induction of antibodies that inhibit erythrocyte invasion.
Kocken, Clemens H M, Withers-Martinez, Chrislaine, Dubbeld, Martin A, van der Wel, Annemarie, Hackett, Fiona, Valderrama, Augusto, Blackman, Michael J, Thomas, Alan W · 2002 · Infection and immunity · DOI
Full title: High-level expression of the malaria blood-stage vaccine candidate Plasmodium falciparum apical membrane antigen 1 and induction of antibodies that inhibit erythrocyte invasion.
Authors: Kocken, Clemens H M, Withers-Martinez, Chrislaine, Dubbeld, Martin A, van der Wel, Annemarie, Hackett, Fiona, Valderrama, Augusto, Blackman, Michael J, Thomas, Alan W
PaperProduction of single-chain variable fragment antibody (scFv) in fed-batch and continuous culture of Pichia pastoris by two different methanol feeding methods.
Yamawaki, Shinya, Matsumoto, Takehiro, Ohnishi, Yuka, Kumada, Yoichi, Shiomi, Naofumi, Katsuda, Tomohisa, Lee, Eun Kyu, Katoh, Shigeo · 2007 · Journal of bioscience and bioengineering · DOI
Full title: Production of single-chain variable fragment antibody (scFv) in fed-batch and continuous culture of Pichia pastoris by two different methanol feeding methods.
PaperEvaluating Pulses and Modified Fed-batch Feeding of Methanol to Increase Expression Level of Human Insulin Precursor in Pichia Pastoris High-Density Cultivation
Putro, Eko Wahyu, Nurdiani, Dini, Hariyatun, Utami, Nuruliawaty, Kusharyoto, Wien, Juanssilfero, Ario Betha, Silvia, Rozy Ayu · 2022 · International Journal on Advanced Science Engineering and Information Technology · DOI
Full title: Evaluating Pulses and Modified Fed-batch Feeding of Methanol to Increase Expression Level of Human Insulin Precursor in Pichia Pastoris High-Density Cultivation
Full title: Cofactor engineering for efficient production of α-farnesene by rational modification of NADPH and ATP regeneration pathway in Pichia pastoris
PaperA comparison of different constitutive expression systems for the production of bovine chymosin in Komagataella phaffii
Barros, Roberta F, Marco, Janice Lisboa De, Piva, Luíza Cesca, Coelho, Cíntia Marques, Moraes, Lídia Maria Pepe de, Torres, Fernando Araripe Gonçalves · 2025 · Journal of Dairy Science · DOI
Full title: A comparison of different constitutive expression systems for the production of bovine chymosin in Komagataella phaffii
Authors: Barros, Roberta F, Marco, Janice Lisboa De, Piva, Luíza Cesca, Coelho, Cíntia Marques, Moraes, Lídia Maria Pepe de, Torres, Fernando Araripe Gonçalves
PaperImplementing Chemostat Fermentation of Pichia Pastoris Producing Recombinant HBsAg to Optimize Cell Density Affected by Methanol Rate
Rahimi, Alireza, Karimi, Afzal, Hosseini, Seyed Mohammad, Aghdasinia, Hassan, Mianroodi, Reza Arabi, Nieto-Taype, M, Garcia-Ortega, X, Albiol, J, Montesinos-Segu, J, Valero, F, Muoz, M, Rincn, J Juregui, Carren, L, Vela, N, Karbalaei, M, Rezaee, S, Farsiani, H, Rahimi, A, Hosseini, S, Javidanbardan, A, Khatami, M, Rahimi, A, Hosseini, S, Karimi, A, Aghdasinia, H, Mianroodi, R Arabi, Hosseini, S, Javidanbardan, A, Khatami, M, Tripathi, N, Shrivastava, A, Oak, A, Jansen, G, Chan, C, Cregg, J, Cereghino, J, Shi, J, Higgins, D, Cos, O, Ramn, R, Montesinos, J, Valero, F, James, A, Morvarid, A, Zeenathul, N, Tam, Y, Zuridah, H, Mohd-Azmi, M, Azizon, B, Gurramkonda, C, Adnan, A, Gbel, T, Lnsdorf, H, Ross, A, Nemani, S, Swaminathan, S, Khanna, N, Rinas, U, Cereghino, G, Cereghino, J, Ilgen, C, Cregg, J, Julien, C, Singh, A, Narang, A, Takagi, S, Tsutsumi, N, Terui, Y, Kong, X, Yurimoto, H, Sakai, Y, Hardy, E, Martnez, E, Diago, D, Daz, R, Gonzlez, D, Herrera, L, Hosseini, S, Sarvari, T, Bashiri, G, Khatami, M, Shojaosadati, S, Yazdani, R, Shams-Bakhsh, M, Hassani-Mehraban, A, Arab, S, Thelen, N, Thiry, M, Crommen, J, Fillet, M, Jacobs, N, Brans, A, Servais, A.-C, Tam, Y, Allaudin, Z, Lila, M, Bahaman, A, Tan, J, Rezaei, M, Chauhan, A · 2021 · Biointerface Research in Applied Chemistry · DOI
Full title: Implementing Chemostat Fermentation of Pichia Pastoris Producing Recombinant HBsAg to Optimize Cell Density Affected by Methanol Rate
Authors: Rahimi, Alireza, Karimi, Afzal, Hosseini, Seyed Mohammad, Aghdasinia, Hassan, Mianroodi, Reza Arabi, Nieto-Taype, M, Garcia-Ortega, X, Albiol, J, Montesinos-Segu, J, Valero, F, Muoz, M, Rincn, J Juregui, Carren, L, Vela, N, Karbalaei, M, Rezaee, S, Farsiani, H, Rahimi, A, Hosseini, S, Javidanbardan, A, Khatami, M, Rahimi, A, Hosseini, S, Karimi, A, Aghdasinia, H, Mianroodi, R Arabi, Hosseini, S, Javidanbardan, A, Khatami, M, Tripathi, N, Shrivastava, A, Oak, A, Jansen, G, Chan, C, Cregg, J, Cereghino, J, Shi, J, Higgins, D, Cos, O, Ramn, R, Montesinos, J, Valero, F, James, A, Morvarid, A, Zeenathul, N, Tam, Y, Zuridah, H, Mohd-Azmi, M, Azizon, B, Gurramkonda, C, Adnan, A, Gbel, T, Lnsdorf, H, Ross, A, Nemani, S, Swaminathan, S, Khanna, N, Rinas, U, Cereghino, G, Cereghino, J, Ilgen, C, Cregg, J, Julien, C, Singh, A, Narang, A, Takagi, S, Tsutsumi, N, Terui, Y, Kong, X, Yurimoto, H, Sakai, Y, Hardy, E, Martnez, E, Diago, D, Daz, R, Gonzlez, D, Herrera, L, Hosseini, S, Sarvari, T, Bashiri, G, Khatami, M, Shojaosadati, S, Yazdani, R, Shams-Bakhsh, M, Hassani-Mehraban, A, Arab, S, Thelen, N, Thiry, M, Crommen, J, Fillet, M, Jacobs, N, Brans, A, Servais, A.-C, Tam, Y, Allaudin, Z, Lila, M, Bahaman, A, Tan, J, Rezaei, M, Chauhan, A
Venue: Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Paper[Engineering of a System for the Production of Mutant Human Alpha-Fetoprotein in the Methylotrophic Yeast Pichia pastoris].
Morozkina, E V, Vavilova, E A, Zatsepin, S S, Klyachko, E V, Yagudin, T A, Chulkin, A M, Dudich, I V, Semenkova, L N, Churilova, I V, Benevolenskii, S V · 2016 · Prikladnaia biokhimiia i mikrobiologiia
Full title: [Engineering of a System for the Production of Mutant Human Alpha-Fetoprotein in the Methylotrophic Yeast Pichia pastoris].
Authors: Morozkina, E V, Vavilova, E A, Zatsepin, S S, Klyachko, E V, Yagudin, T A, Chulkin, A M, Dudich, I V, Semenkova, L N, Churilova, I V, Benevolenskii, S V
Year: 2016
Venue: Prikladnaia biokhimiia i mikrobiologiia
Paper[Expression of H5N1 avian influenza virus haemagglutinin protein in pichia pastoris by high-density cell fermentation].
Yang, Kunyu, He, Fangping, Li, Shaowei, Zhang, Jiahong, Lin, Qingshan, Chen, Zhenqin, Li, Zhongyi, Zhang, Jun, Xia, Ningshao · 2009 · Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Full title: [Expression of H5N1 avian influenza virus haemagglutinin protein in pichia pastoris by high-density cell fermentation].
ThesisEffect of volumetric oxygen transfer coefficient (kLa) on ethanol production performance by Scheffersomyces stipitis on hemicellulosic sugarcane bagasse hydrolysate
Felipe, María Das Graças De Almeida · 2016 · Theses · DOI
Full title: Effect of volumetric oxygen transfer coefficient (kLa) on ethanol production performance by Scheffersomyces stipitis on hemicellulosic sugarcane bagasse hydrolysate
ThesisAvaliation of the induction time and metanol concentration in the expresión of L-asparaginase II from Saccharomyces cerevisiae using Pichia pastoris ('Mut POT. S')
Vitolo, Michele · 2016 · Universidade de São Paulo – USP Digital Library · DOI
Full title: Avaliation of the induction time and metanol concentration in the expresión of L-asparaginase II from Saccharomyces cerevisiae using Pichia pastoris ('Mut POT. S')
Authors: Vitolo, Michele
Year: 2016
Venue: Universidade de São Paulo – USP Digital Library
ThesisAn experimental and modeling approach for ethanol production by Kluyveromyces marxianus in stirred tank bioreactor using vacuum extraction as a strategy to overcome product inhibition
Full title: An experimental and modeling approach for ethanol production by Kluyveromyces marxianus in stirred tank bioreactor using vacuum extraction as a strategy to overcome product inhibition
ThesisMetabolic engineering of glycosylation and secretion pathways in Pichia pastoris and its application in production of the human recombinant enzyme N-acetylgalactosamine-6-sulfate sulfatase
Rodríguez López, Edwin Alexander · 2018 · Ghent University (Universiteit Gent) · DOI
Full title: Metabolic engineering of glycosylation and secretion pathways in Pichia pastoris and its application in production of the human recombinant enzyme N-acetylgalactosamine-6-sulfate sulfatase
ThesisComparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain
Brady, Joseph R; Whittaker, Charles A; Tan, Melody C et al. · 2021 · Theses · DOI
Full title: Comparative genome‐scale analysis of Pichia pastoris variants informs selection of an optimal base strain
Authors: Brady, Joseph R; Whittaker, Charles A; Tan, Melody C; Kristensen, D Lee; Ma, Duanduan; Dalvie, Neil C; Love, Kerry Routenberg; Love, J Christopher
ThesisCaractérisation quantitative de la croissance et du système d’expression d’une souche recombinante de Pichia pastoris Mut+: influence du taux de dilution et du type de substrat
Full title: Caractérisation quantitative de la croissance et du système d’expression d’une souche recombinante de Pichia pastoris Mut+: influence du taux de dilution et du type de substrat
ThesisMixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures
Jungo, Carmen; Marison, Ian; von Stockar, Urs · 2007 · Theses · DOI
Full title: Mixed feeds of glycerol and methanol can improve the performance of Pichia pastoris cultures: A quantitative study based on concentration gradients in transient continuous cultures
Authors: Jungo, Carmen; Marison, Ian; von Stockar, Urs
ThesisA quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris
Jungo, Carmen; Schenk, Jonas; Pasquier, Muriel et al. · 2007 · Theses · DOI
Full title: A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris
ThesisShaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000L by surface aeration with air
Zhang, X.; Stettler, M.; Reif, O. et al. · 2009 · Theses · DOI
Full title: Shaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000L by surface aeration with air
Authors: Zhang, X.; Stettler, M.; Reif, O.; Kocourek, A.; Dejesus, M.; Hacker, D.; Wurm, F.
Thesis**Institution:** École polytechnique fédérale de Lausanne (EPFL) **Date:** 2009-09-01T12:18:47
Zhang, X.; Bürki, C.A.; Stettler, M. et al. · 2009 · Theses · DOI
Full title: **Institution:** École polytechnique fédérale de Lausanne (EPFL) **Date:** 2009-09-01T12:18:47
Authors: Zhang, X.; Bürki, C.A.; Stettler, M.; De Sanctis, D.; Perrone, M.; Discacciati, M.; Parolini, N.; De Jesus, M.; Hacker, D.L.; Quarteroni, A.; Wurm, F.M.
ThesisInfluence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut(+) strain
Schenk, Jonas; Balazs, Krisztina; Jungo, Carmen et al. · 2010 · Theses · DOI
Full title: Influence of specific growth rate on specific productivity and glycosylation of a recombinant avidin produced by a Pichia pastoris Mut(+) strain
Authors: Schenk, Jonas; Balazs, Krisztina; Jungo, Carmen; Urfer, Julien; Wegmann, Carole; Zocchi, Andrea; Marison, Ian W.; von Stockar, Urs
ThesisA comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation
Monteil, Dominique T.; Juvet, Valentin; Paz, Jonathan et al. · 2017 · Theses · DOI
Full title: A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation
Authors: Monteil, Dominique T.; Juvet, Valentin; Paz, Jonathan; Moniatte, Marc; Baldi, Lucia; Hacker, David L.; Wurm, Florian M.
ThesisHeat flux as an on-line indicator of metabolic activity in pilot scale bioreactor during the production of Bacillus thuringiensis var. galleriae-based biopesticides
Anderson, R. K. I.; Jayaraman, Kunthala; Voisard, D. et al. · 2006 · Theses · DOI
Full title: Heat flux as an on-line indicator of metabolic activity in pilot scale bioreactor during the production of Bacillus thuringiensis var. galleriae-based biopesticides
Authors: Anderson, R. K. I.; Jayaraman, Kunthala; Voisard, D.; Marison, Ian W.; von Stockar, U.
ThesisQuantitative characterization of the regulation of the synthesis of alcohol oxidase and of the expression of recombinant avidin in a Pichia pastoris Mut+ strain
Jungo, Carmen; Rérat, Claude; Marison, Ian et al. · 2007 · École polytechnique fédérale de Lausanne (EPFL) · DOI
Full title: Quantitative characterization of the regulation of the synthesis of alcohol oxidase and of the expression of recombinant avidin in a Pichia pastoris Mut+ strain
Authors: Jungo, Carmen; Rérat, Claude; Marison, Ian; von Stockar, Urs
Year: 2007
Venue: École polytechnique fédérale de Lausanne (EPFL)
ThesisHigh-cell-density cultivation and recombinant protein production with Komagataella pastoris in stirred-tank bioreactors from milliliter to cubic meter scale
Schmideder, Andreas; Hensler, Samantha; Lang, Marina et al. · 2016 · Theses · DOI
Full title: High-cell-density cultivation and recombinant protein production with Komagataella pastoris in stirred-tank bioreactors from milliliter to cubic meter scale
ThesisInnovative methods of knowledge-driven methanol feedback control and state estimation for recombinant Pichia pastoris fed-batch cultivation
Brunner, V., Metzenmacher, M., Birle, S., Geier, D., Becker, T. · 2017 · Theses · DOI
Full title: Innovative methods of knowledge-driven methanol feedback control and state estimation for recombinant Pichia pastoris fed-batch cultivation
Authors: Brunner, V., Metzenmacher, M., Birle, S., Geier, D., Becker, T.
ThesisExperimental and in-silico investigation of population heterogeneity in continuous Sachharomyces cerevisiae scale-down fermentation in a novel two-compartment setup
Heins, Anna-Lena; Lencastre Fernandes, Rita; Gernaey, Krist et al. · 2015 · Theses · DOI
Full title: Experimental and in-silico investigation of population heterogeneity in continuous Sachharomyces cerevisiae scale-down fermentation in a novel two-compartment setup
ThesisMulti-scale modeling for prediction of distributed cellular properties in response to substrate spatial gradients in a continuously run microreactor
Lencastre Fernandes, Rita; Krühne, Ulrich; id_orcid 0000-0001-7774-7442 et al. · 2012 · Theses · DOI
Full title: Multi-scale modeling for prediction of distributed cellular properties in response to substrate spatial gradients in a continuously run microreactor
ThesisImpact of Elevated Levels of Dissolved CO 2 on Performance and Proteome Response of an Industrial 2'-Fucosyllactose Producing Escherichia coli Strain
Gecse, Greta; Vente, André; Kilstrup, Mogens et al. · 2022 · Technical University of Denmark (DTU) · DOI
Full title: Impact of Elevated Levels of Dissolved CO 2 on Performance and Proteome Response of an Industrial 2'-Fucosyllactose Producing Escherichia coli Strain
ThesisBurden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan et al. · 2022 · Theses · DOI
Full title: Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Authors: Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan; Jensen, Michael Krogh; id_orcid 0000-0001-7574-4707; Workman, Christopher T.; id_orcid 0000-0002-2210-3743
ThesisMinimizing acetate formation from overflow metabolism in <i>Escherichia coli</i>: comparison of genetic engineering strategies to improve robustness toward sugar gradients in large-scale fermentation processes
Gecse, Greta; Labunskaite, Rugile; id_orcid 0009-0007-8147-9023 et al. · 2024 · Theses · DOI
Full title: Minimizing acetate formation from overflow metabolism in <i>Escherichia coli</i>: comparison of genetic engineering strategies to improve robustness toward sugar gradients in large-scale fermentation processes
ThesisImpact of cellular stress on recombinant monoclonal antibody produced by high yielding Chinese Hamster Ovary (CHO) cell cultures in bioreactors
Chevallier, Valentine · 2020 · Technical University of Denmark (DTU) · DOI
Full title: Impact of cellular stress on recombinant monoclonal antibody produced by high yielding Chinese Hamster Ovary (CHO) cell cultures in bioreactors
Full title: Characterisation and optimisation of <i>Escherichia coli</i> cell factories for large-scale industrial production of human milk oligosaccharides
ThesisModelling of industrial-scale bioreactors using the particle lifeline approach
Nadal-Rey, Gisela; Kavanagh, John M.; Cassells, Benny et al. · 2023 · Theses · DOI
Full title: Modelling of industrial-scale bioreactors using the particle lifeline approach
Authors: Nadal-Rey, Gisela; Kavanagh, John M.; Cassells, Benny; Cornelissen, Sjef; Fletcher, David F.; Gernaey, Krist V.; id_orcid 0000-0002-0364-1773; McClure, Dale D.
ThesisIntegrated Optimization of Hemoprotein Biomanufacturing in Yeast:From Upstream Engineering of Heme Synthesis to Downstream Purification of Human Hemoglobin
Full title: Integrated Optimization of Hemoprotein Biomanufacturing in Yeast:From Upstream Engineering of Heme Synthesis to Downstream Purification of Human Hemoglobin
ThesisApplication of a Mechanistic Model as a Tool for On-line Monitoring of Pilot Scale Filamentous Fungal Fermentation Processes - The Importance of Evaporation Effects:Mechanistic model for pilot scale monitoring
Mears, Lisa; Stocks, Stuart M.; Albæk, Mads Orla et al. · 2017 · Theses · DOI
Full title: Application of a Mechanistic Model as a Tool for On-line Monitoring of Pilot Scale Filamentous Fungal Fermentation Processes - The Importance of Evaporation Effects:Mechanistic model for pilot scale monitoring
Authors: Mears, Lisa; Stocks, Stuart M.; Albæk, Mads Orla; Sin, Gürkan; id_orcid 0000-0003-0513-4502; Gernaey, Krist V.; id_orcid 0000-0002-0364-1773
ThesisDynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica
Kerssemakers, Abraham Antonius Johannes; id_orcid 0000-0002-3462-4642; Øzmerih, Süleyman et al. · 2023 · Theses · DOI
Full title: Dynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica
Authors: Kerssemakers, Abraham Antonius Johannes; id_orcid 0000-0002-3462-4642; Øzmerih, Süleyman; Sin, Gürkan; id_orcid 0000-0003-0513-4502; Sudarsan, Suresh
ThesisComparison of Different Strategies for Selection/Adaptation of Mixed Microbial Cultures Able to Ferment Crude Glycerol Derived from Second-Generation Biodiesel
Varrone, Cristiano; Heggeset, T. M. B.; Le, S. B. et al. · 2015 · Theses · DOI
Full title: Comparison of Different Strategies for Selection/Adaptation of Mixed Microbial Cultures Able to Ferment Crude Glycerol Derived from Second-Generation Biodiesel
Authors: Varrone, Cristiano; Heggeset, T. M. B.; Le, S. B.; Haugen, T.; Markussen, S.; V. Skiadas, Ioannis; id_orcid 0000-0001-6183-4592; Gavala, Hariklia N.; id_orcid 0000-0002-3924-5334
ThesisUnderstanding gradients in industrial bioreactors
Nadal-Rey, Gisela; McClure, Dale D.; Kavanagh, John M. et al. · 2021 · Theses · DOI
Full title: Understanding gradients in industrial bioreactors
Authors: Nadal-Rey, Gisela; McClure, Dale D.; Kavanagh, John M.; Cornelissen, Sjef; Fletcher, David F.; Gernaey, Krist V.; id_orcid 0000-0002-0364-1773
ThesisFlow-informed clustering of bioreactor volumes to build CFD-based compartment models
Le Nepvou De Carfort, Johan; Puig I Laborda, Víctor; Nielsen, Lars Keld et al. · 2025 · Theses · DOI
Full title: Flow-informed clustering of bioreactor volumes to build CFD-based compartment models
Authors: Le Nepvou De Carfort, Johan; Puig I Laborda, Víctor; Nielsen, Lars Keld; Gernaey, Krist V.; id_orcid 0000-0002-0364-1773; Krühne, Ulrich; id_orcid 0000-0001-7774-7442
ThesisUse of Image Analysis to Understand Enzyme Stability in an Aerated Stirred Reactor
Dias Gomes, Mafalda; Moiseyenko, Rayisa P.; Baum, Andreas et al. · 2019 · Technical University of Denmark (DTU) · DOI
Full title: Use of Image Analysis to Understand Enzyme Stability in an Aerated Stirred Reactor
Authors: Dias Gomes, Mafalda; Moiseyenko, Rayisa P.; Baum, Andreas; id_orcid 0000-0003-1552-0220; Jørgensen, Thomas M.; id_orcid 0000-0003-2920-1343; Woodley, John M.; id_orcid 0000-0002-7976-2483
ThesisRecombinant β-Carotene Production by Yarrowia lipolytica - Assessing the Potential of Micro-Scale Fermentation Analysis in Cell Factory Design and Bioreaction Optimization
Jacobsen, Irene Hjorth; Ledesma-Amaro, Rodrigo; Martinez, José Luis et al. · 2020 · Technical University of Denmark (DTU) · DOI
Full title: Recombinant β-Carotene Production by Yarrowia lipolytica - Assessing the Potential of Micro-Scale Fermentation Analysis in Cell Factory Design and Bioreaction Optimization
ThesisProduction of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA: ethanol O-acyltransferase Eht1 or Eeb1
Zhuang, Shiwen; Fu, Junshu; Powell, Chris et al. · 2015 · Technical University of Denmark (DTU) · DOI
Full title: Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA: ethanol O-acyltransferase Eht1 or Eeb1
ThesisExperimental and in-silico investigation of population heterogeneity in continuous Sachharomyces cerevisiae scale-down fermentation in a novel two-compartment setup
Heins, Anna-Lena; Lencastre Fernandes, Rita; Gernaey, Krist et al. · 2015 · Theses · DOI
Full title: Experimental and in-silico investigation of population heterogeneity in continuous Sachharomyces cerevisiae scale-down fermentation in a novel two-compartment setup
ThesisMulti-scale modeling for prediction of distributed cellular properties in response to substrate spatial gradients in a continuously run microreactor
Lencastre Fernandes, Rita; Krühne, Ulrich; id_orcid 0000-0001-7774-7442 et al. · 2012 · Theses · DOI
Full title: Multi-scale modeling for prediction of distributed cellular properties in response to substrate spatial gradients in a continuously run microreactor
ThesisTowards Modelling of an Industrial <i>Aspergillus oryzae </i>Aerobic Fed-batch Fermentation Process – Process Characterization Across Scales
Albino, Mariana; L. Gargalo, C; id_orcid 0000-0002-8740-9591 et al. · 2024 · Theses · DOI
Full title: Towards Modelling of an Industrial <i>Aspergillus oryzae </i>Aerobic Fed-batch Fermentation Process – Process Characterization Across Scales
ThesisImpact of Elevated Levels of Dissolved CO 2 on Performance and Proteome Response of an Industrial 2'-Fucosyllactose Producing Escherichia coli Strain
Gecse, Greta; Vente, André; Kilstrup, Mogens et al. · 2022 · Technical University of Denmark (DTU) · DOI
Full title: Impact of Elevated Levels of Dissolved CO 2 on Performance and Proteome Response of an Industrial 2'-Fucosyllactose Producing Escherichia coli Strain
ThesisComparison of Traditional Rushton Disc Turbines with Up-pumping Hydrofoil B2 Impellers in 550 L Pilot Scale Aerobic Submerged Fermentations
Albæk, Mads Orla; Gernaey, Krist; id_orcid 0000-0002-0364-1773 et al. · 2010 · Theses · DOI
Full title: Comparison of Traditional Rushton Disc Turbines with Up-pumping Hydrofoil B2 Impellers in 550 L Pilot Scale Aerobic Submerged Fermentations
Authors: Albæk, Mads Orla; Gernaey, Krist; id_orcid 0000-0002-0364-1773; Hansen, M.S.; Stocks, Stuart M.
ThesisComparison of Traditional Rushton Disc Turbines with Up-pumping Hydrofoil B2 Impellers in 550 L Pilot Scale Aerobic Submerged Fermentations
Albæk, Mads Orla; Stocks, Stuart M.; Nienow, Alvin W. · 2009 · Theses · DOI
Full title: Comparison of Traditional Rushton Disc Turbines with Up-pumping Hydrofoil B2 Impellers in 550 L Pilot Scale Aerobic Submerged Fermentations
Authors: Albæk, Mads Orla; Stocks, Stuart M.; Nienow, Alvin W.
ThesisDifferential Gene Expression Profiles and Real-Time Measurements of Growth Parameters in Saccharomyces cerevisiae Grown in Microliter- Scale Bioreactors Equipped with Internal Stirring
Boccazzio, P; Zhang, Z; Kurosawa, K et al. · 2006 · Theses · DOI
Full title: Differential Gene Expression Profiles and Real-Time Measurements of Growth Parameters in Saccharomyces cerevisiae Grown in Microliter- Scale Bioreactors Equipped with Internal Stirring
Authors: Boccazzio, P; Zhang, Z; Kurosawa, K; Szita, Nicolas; Bhattacharya, S; Jensen, K F; Sinskey, A J
ThesisBurden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan et al. · 2022 · Theses · DOI
Full title: Burden Imposed by Heterologous Protein Production in Two Major Industrial Yeast Cell Factories: Identifying Sources and Mitigation Strategies
Authors: Kastberg, Louise La Barbera; id_orcid 0000-0001-9291-3824; Ard, Ryan; Jensen, Michael Krogh; id_orcid 0000-0001-7574-4707; Workman, Christopher T.; id_orcid 0000-0002-2210-3743
ThesisMinimizing acetate formation from overflow metabolism in <i>Escherichia coli</i>: comparison of genetic engineering strategies to improve robustness toward sugar gradients in large-scale fermentation processes
Gecse, Greta; Labunskaite, Rugile; id_orcid 0009-0007-8147-9023 et al. · 2024 · Theses · DOI
Full title: Minimizing acetate formation from overflow metabolism in <i>Escherichia coli</i>: comparison of genetic engineering strategies to improve robustness toward sugar gradients in large-scale fermentation processes
ThesisImpact of cellular stress on recombinant monoclonal antibody produced by high yielding Chinese Hamster Ovary (CHO) cell cultures in bioreactors
Chevallier, Valentine · 2020 · Technical University of Denmark (DTU) · DOI
Full title: Impact of cellular stress on recombinant monoclonal antibody produced by high yielding Chinese Hamster Ovary (CHO) cell cultures in bioreactors
Full title: Characterisation and optimisation of <i>Escherichia coli</i> cell factories for large-scale industrial production of human milk oligosaccharides
ThesisModelling of industrial-scale bioreactors using the particle lifeline approach
Nadal-Rey, Gisela; Kavanagh, John M.; Cassells, Benny et al. · 2023 · Theses · DOI
Full title: Modelling of industrial-scale bioreactors using the particle lifeline approach
Authors: Nadal-Rey, Gisela; Kavanagh, John M.; Cassells, Benny; Cornelissen, Sjef; Fletcher, David F.; Gernaey, Krist V.; id_orcid 0000-0002-0364-1773; McClure, Dale D.
ThesisAutomated Electrochemical Glucose Biosensor Platform as an Efficient Tool Toward On-Line Fermentation Monitoring:Novel Application Approaches and Insights
Pontius, Katrin; Semenova, Daria; Silina, Yuliya E. et al. · 2020 · Theses · DOI
Full title: Automated Electrochemical Glucose Biosensor Platform as an Efficient Tool Toward On-Line Fermentation Monitoring:Novel Application Approaches and Insights
ThesisProduction of enzymatically active recombinant full-length barley high pI alpha-glucosidase of glycoside family 31 by high cell-density fermentation of Pichia pastoris and affinity purification
Næsted, Henrik; Kramhøft, Birte; Lok, F. et al. · 2006 · Technical University of Denmark (DTU) · DOI
Full title: Production of enzymatically active recombinant full-length barley high pI alpha-glucosidase of glycoside family 31 by high cell-density fermentation of Pichia pastoris and affinity purification
ThesisIntegrated Optimization of Hemoprotein Biomanufacturing in Yeast:From Upstream Engineering of Heme Synthesis to Downstream Purification of Human Hemoglobin
Full title: Integrated Optimization of Hemoprotein Biomanufacturing in Yeast:From Upstream Engineering of Heme Synthesis to Downstream Purification of Human Hemoglobin
ThesisUse of continuous lactose fermentation for ethanol production by <em>Kluveromyces marxianus</em> for verification and extension of a biochemically structured model
Sansonetti, S.; Hobley, Timothy John; id_orcid 0000-0002-1355-6214 et al. · 2013 · Theses · DOI
Full title: Use of continuous lactose fermentation for ethanol production by <em>Kluveromyces marxianus</em> for verification and extension of a biochemically structured model
ThesisScale-up Modeling of a Pharmaceutical Antisolvent Crystallization via a Hybrid Method of Computational Fluid Dynamics and Compartmental Modeling
Öner, Merve; Stocks, Stuart M.; Abildskov, Jens et al. · 2019 · Theses · DOI
Full title: Scale-up Modeling of a Pharmaceutical Antisolvent Crystallization via a Hybrid Method of Computational Fluid Dynamics and Compartmental Modeling
Authors: Öner, Merve; Stocks, Stuart M.; Abildskov, Jens; id_orcid 0000-0003-1187-8778; Sin, Gürkan; id_orcid 0000-0003-0513-4502
ThesisReal-time direct cell concentration and viability determination using a fully automated microfluidic platform for standalone process monitoring
Rodrigues de Sousa Nunes, Pedro André; Kjaerulff, S.; Dufva, Martin et al. · 2015 · Theses · DOI
Full title: Real-time direct cell concentration and viability determination using a fully automated microfluidic platform for standalone process monitoring
Authors: Rodrigues de Sousa Nunes, Pedro André; Kjaerulff, S.; Dufva, Martin; id_orcid 0000-0001-5449-0189; Mogensen, Klaus Bo
ThesisComparison of Traditional Rushton Disc Turbines with Up-pumping Hydrofoil B2 Impellers in 550 L Pilot Scale Aerobic Submerged Fermentations
Albæk, Mads Orla; Gernaey, Krist; id_orcid 0000-0002-0364-1773 et al. · 2010 · Theses · DOI
Full title: Comparison of Traditional Rushton Disc Turbines with Up-pumping Hydrofoil B2 Impellers in 550 L Pilot Scale Aerobic Submerged Fermentations
Authors: Albæk, Mads Orla; Gernaey, Krist; id_orcid 0000-0002-0364-1773; Hansen, M.S; Stocks, Stuart M.
ThesisApplication of a Mechanistic Model as a Tool for On-line Monitoring of Pilot Scale Filamentous Fungal Fermentation Processes - The Importance of Evaporation Effects:Mechanistic model for pilot scale monitoring
Mears, Lisa; Stocks, Stuart M.; Albæk, Mads Orla et al. · 2017 · Theses · DOI
Full title: Application of a Mechanistic Model as a Tool for On-line Monitoring of Pilot Scale Filamentous Fungal Fermentation Processes - The Importance of Evaporation Effects:Mechanistic model for pilot scale monitoring
Authors: Mears, Lisa; Stocks, Stuart M.; Albæk, Mads Orla; Sin, Gürkan; id_orcid 0000-0003-0513-4502; Gernaey, Krist V.; id_orcid 0000-0002-0364-1773
ThesisDynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica
Kerssemakers, Abraham Antonius Johannes; id_orcid 0000-0002-3462-4642; Øzmerih, Süleyman et al. · 2023 · Theses · DOI
Full title: Dynamic Interplay between O2 Availability, Growth Rates, and the Transcriptome of Yarrowia lipolytica
Authors: Kerssemakers, Abraham Antonius Johannes; id_orcid 0000-0002-3462-4642; Øzmerih, Süleyman; Sin, Gürkan; id_orcid 0000-0003-0513-4502; Sudarsan, Suresh
ThesisUnderstanding gradients in industrial bioreactors
Nadal-Rey, Gisela; McClure, Dale D.; Kavanagh, John M. et al. · 2021 · Theses · DOI
Full title: Understanding gradients in industrial bioreactors
Authors: Nadal-Rey, Gisela; McClure, Dale D.; Kavanagh, John M.; Cornelissen, Sjef; Fletcher, David F.; Gernaey, Krist V.; id_orcid 0000-0002-0364-1773
ThesisFlow-informed clustering of bioreactor volumes to build CFD-based compartment models
Le Nepvou De Carfort, Johan; Puig I Laborda, Víctor; Nielsen, Lars Keld et al. · 2025 · Theses · DOI
Full title: Flow-informed clustering of bioreactor volumes to build CFD-based compartment models
Authors: Le Nepvou De Carfort, Johan; Puig I Laborda, Víctor; Nielsen, Lars Keld; Gernaey, Krist V.; id_orcid 0000-0002-0364-1773; Krühne, Ulrich; id_orcid 0000-0001-7774-7442
ThesisUse of Image Analysis to Understand Enzyme Stability in an Aerated Stirred Reactor
Dias Gomes, Mafalda; Moiseyenko, Rayisa P.; Baum, Andreas et al. · 2019 · Technical University of Denmark (DTU) · DOI
Full title: Use of Image Analysis to Understand Enzyme Stability in an Aerated Stirred Reactor
Authors: Dias Gomes, Mafalda; Moiseyenko, Rayisa P.; Baum, Andreas; id_orcid 0000-0003-1552-0220; Jørgensen, Thomas M.; id_orcid 0000-0003-2920-1343; Woodley, John M.; id_orcid 0000-0002-7976-2483
ThesisRecombinant β-Carotene Production by Yarrowia lipolytica - Assessing the Potential of Micro-Scale Fermentation Analysis in Cell Factory Design and Bioreaction Optimization
Jacobsen, Irene Hjorth; Ledesma-Amaro, Rodrigo; Martinez, José Luis et al. · 2020 · Technical University of Denmark (DTU) · DOI
Full title: Recombinant β-Carotene Production by Yarrowia lipolytica - Assessing the Potential of Micro-Scale Fermentation Analysis in Cell Factory Design and Bioreaction Optimization
ThesisProduction of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA: ethanol O-acyltransferase Eht1 or Eeb1
Zhuang, Shiwen; Fu, Junshu; Powell, Chris et al. · 2015 · Technical University of Denmark (DTU) · DOI
Full title: Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular expression of Saccharomyces cerevisiae acyl-CoA: ethanol O-acyltransferase Eht1 or Eeb1
ThesisEffect of volumetric oxygen transfer coefficient (kLa) on ethanol production performance by Scheffersomyces stipitis on hemicellulosic sugarcane bagasse hydrolysate
Silva, Débora Danielle Virgínio; Dussán, Kelly Johana [UNESP]; Hernández, Valentina et al. · 2018 · Theses · DOI
Full title: Effect of volumetric oxygen transfer coefficient (kLa) on ethanol production performance by Scheffersomyces stipitis on hemicellulosic sugarcane bagasse hydrolysate
Authors: Silva, Débora Danielle Virgínio; Dussán, Kelly Johana [UNESP]; Hernández, Valentina; Silva, Silvio Silvério da; Cardona, Carlos Ariel; Felipe, Maria das Graças de Almeida
ThesisImproving the Environmental Sustainability of Polyketides Colorants Production by Talaromyces Strain through Better Hydrodynamic Design in Bioreactors
De Oliveira, Fernanda [UNESP]; Zapata-Boada, Santiago; Da Silva, S. S. et al. · 2023 · Theses · DOI
Full title: Improving the Environmental Sustainability of Polyketides Colorants Production by Talaromyces Strain through Better Hydrodynamic Design in Bioreactors
Authors: De Oliveira, Fernanda [UNESP]; Zapata-Boada, Santiago; Da Silva, S. S.; Cuéllar-Franca, Rosa M.; Santos-Ebinuma, Valéria C. [UNESP]
ThesisOxygen transfer and gas holdup in airlift bioreactors assembled with helical flow promoters
Picão, Bruno W. [UNESP]; Gonçalves, Daniele O. [UNESP]; Ribeiro, Renata M. M. G. P. et al. · 2023 · Theses · DOI
Full title: Oxygen transfer and gas holdup in airlift bioreactors assembled with helical flow promoters
Authors: Picão, Bruno W. [UNESP]; Gonçalves, Daniele O. [UNESP]; Ribeiro, Renata M. M. G. P.; Esperança, Mateus N.; Peixoto, Guilherme [UNESP]; Cerri, Marcel O. [UNESP]
ThesisHeterologous expression of GH11 xylanase from Myceliophthora heterothallica F.2.1.4 in Pichia pastoris
de Amo, Gabriela Salvador; Bezerra-Bussoli, Carolina [UNESP]; Rodrigues da Silva, Ronivaldo [UNESP] et al. · 2025 · Universidade Estadual Paulista (UNESP) · DOI
Full title: Heterologous expression of GH11 xylanase from Myceliophthora heterothallica F.2.1.4 in Pichia pastoris
Authors: de Amo, Gabriela Salvador; Bezerra-Bussoli, Carolina [UNESP]; Rodrigues da Silva, Ronivaldo [UNESP]; Kishi, Luciano Takeshi; Ferreira, Henrique [UNESP]; Gomes, Eleni [UNESP]; Bonilla-Rodriguez, Gustavo Orlando [UNESP]
ThesisApplication of methylotrophic yeast Pichia pastoris in the field of food industry - A review
Silva, Viviane C. [UNESP]; Peres, Maristela F. S. [UNESP]; Gattas, Edwil Aparecida de Lucca [UNESP] · 2014 · Universidade Estadual Paulista (UNESP) · DOI
Full title: Application of methylotrophic yeast Pichia pastoris in the field of food industry - A review
Authors: Silva, Viviane C. [UNESP]; Peres, Maristela F. S. [UNESP]; Gattas, Edwil Aparecida de Lucca [UNESP]
ThesisCharacterization, Optimization, and Scaling-up of Submerged Inonotus hispidus Mycelial Fermentation for Enhanced Biomass and Polysaccharide Production
Shen, Ke; Liu, Yuanshuai; Liu, Liyan et al. · 2024 · Theses · DOI
Full title: Characterization, Optimization, and Scaling-up of Submerged Inonotus hispidus Mycelial Fermentation for Enhanced Biomass and Polysaccharide Production
Authors: Shen, Ke; Liu, Yuanshuai; Liu, Liyan; Khan, Abdul Waheed; Normakhamatov, Nodirali; Wang, Zhaomei
ThesisImproved Nonlinear Quality Estimation for Multiphase Batch Processes Based on Relevance Vector Machine with Neighborhood Component Variable Selection
Full title: Improved Nonlinear Quality Estimation for Multiphase Batch Processes Based on Relevance Vector Machine with Neighborhood Component Variable Selection
ThesisDevelopment of High Cell Density 30 L Fed-Batch Pichia pastoris Fermentation and Optimization of in situ Methanol Probe Response for Automated Control of Recombinant Protein Expression
Full title: Development of High Cell Density 30 L Fed-Batch Pichia pastoris Fermentation and Optimization of in situ Methanol Probe Response for Automated Control of Recombinant Protein Expression
ThesisDevelopment of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition (SCADA) Software for Automation of High Cell Density Pichia pastoris Fermentations in a 30L Bioreactor
Full title: Development of Glycerol Feedback Control, Cell Density Measurement Device, and Supervisory Control and Data Acquisition (SCADA) Software for Automation of High Cell Density Pichia pastoris Fermentations in a 30L Bioreactor
ThesisTranslational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
Edwards-Jones, B; Aw, R; Barton, GR et al. · 2015 · Theses · DOI
Full title: Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
ThesisProduction and purification of an active CRM197 in Pichia pastoris and its immunological characterization using a Vi-typhoid antigen vaccine
Aw, Rochelle; Ashik, Muktadir R; Islam, Ariful AZM et al. · 2022 · Imperial College London · DOI
Full title: Production and purification of an active CRM197 in Pichia pastoris and its immunological characterization using a Vi-typhoid antigen vaccine
Authors: Aw, Rochelle; Ashik, Muktadir R; Islam, Ariful AZM; Khan, Imran; Mainuddin, Mohammad; Islam, Md Ashraful; Ahasan, Mohammad M; Polizzi, Karen M
ThesisAccelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform
Sawatzki, Annina; Hans, Sebastian; Narayanan, Harini et al. · 2018 · Theses · DOI
Full title: Accelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform
ThesisEstratègies d'operació en el procés de producció de proteïnes heteròlogues en Pichia pastoris: aplicació de tècniques de monitorització i control
Full title: Estratègies d'operació en el procés de producció de proteïnes heteròlogues en Pichia pastoris: aplicació de tècniques de monitorització i control
ThesisMillora en el procés de producció d'una lipasa de Rhizopus oryzae en Pichia pastoris mitjançant tècniques de monitoratge i estratègies de cultiu alternatives
Full title: Millora en el procés de producció d'una lipasa de Rhizopus oryzae en Pichia pastoris mitjançant tècniques de monitoratge i estratègies de cultiu alternatives
ThesisProviding growth-decoupled strategies for recombinant protein production in Komagataella phaffii: from promoter identification to bioprocess scale-up
Full title: Providing growth-decoupled strategies for recombinant protein production in Komagataella phaffii: from promoter identification to bioprocess scale-up
Impact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-state-maintained continuous cultures
Korpys-Woźniak, Paulina, Kubiak, Piotr, Białas, W., Celińska, Ewelina · 2020
Full title: Impact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-state-maintained continuous cultures
Authors: Korpys-Woźniak, Paulina, Kubiak, Piotr, Białas, W., Celińska, Ewelina
Full title: Heterologous expression and optimization of the antimicrobial peptide acidocin 4356 in Komagataella phaffii to target Pseudomonas aeruginosa.
Immunisation with Neospora caninum subunits rsNcSAG4 and rsNcGRA1 (NcSAG4 and NcGRA1 epitopes construct) in BALB/c mice: the profile of the immune response and controlling the vertical transmission
Fidelis, Cintia Fernandes, Araújo, Leandro Silva de, Prates-Patarroyo, Pablo A., Martins-Kalks, Karlos H., Oliveira, Leandro Licursi de, Vilória, Marlene Isabel Vargas, Tafur-Gómez, Gabriel Andrés, Salcedo, Joaquín Hernán Patarroyo · 2023
Full title: Immunisation with Neospora caninum subunits rsNcSAG4 and rsNcGRA1 (NcSAG4 and NcGRA1 epitopes construct) in BALB/c mice: the profile of the immune response and controlling the vertical transmission
Authors: Fidelis, Cintia Fernandes, Araújo, Leandro Silva de, Prates-Patarroyo, Pablo A., Martins-Kalks, Karlos H., Oliveira, Leandro Licursi de, Vilória, Marlene Isabel Vargas, Tafur-Gómez, Gabriel Andrés, Salcedo, Joaquín Hernán Patarroyo
Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae
Espinosa, Monica I., González-García, R. Axayácatl, Valgepea, Kaspar, Plan, Manuel R., Scott, Colin, Pretorius, Isak S., Marcellin, Esteban, Paulsen, Ian T., Williams, Thomas C. · 2020
Full title: Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae
Authors: Espinosa, Monica I., González-García, R. Axayácatl, Valgepea, Kaspar, Plan, Manuel R., Scott, Colin, Pretorius, Isak S., Marcellin, Esteban, Paulsen, Ian T., Williams, Thomas C.
Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Yu, Xiao‐Wei, Sun, Weihong, Wang, Ying-Zheng, Xu, Yan · 2017
Full title: Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
Authors: Yu, Xiao‐Wei, Sun, Weihong, Wang, Ying-Zheng, Xu, Yan
Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells
C. R. Arbeitman, G. Auge, M. Blaustein, L. Bredeston, Enrique S. Corapi, P. Craig, L. A. Cossio, L. Dain, C. D’Alessio, Fernanda Elias, Natalia B Fernández, J. Gasulla, Natalia Gorojovsky, Gustavo E. Gudesblat, Maria Georgina Herrera, L. I. Ibañez, Tommy Idrovo, Matías Iglesias Randon, L. Kamenetzky, A. Nadra, D. Noseda, C. Pavan, María F. Pavan, M. F. Pignataro, Ernesto A. Román, L. Ruberto, N. Rubinstein, Javier Santos, F. Duarte, A. Zelada · 2020
Full title: Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammalian cells
Authors: C. R. Arbeitman, G. Auge, M. Blaustein, L. Bredeston, Enrique S. Corapi, P. Craig, L. A. Cossio, L. Dain, C. D’Alessio, Fernanda Elias, Natalia B Fernández, J. Gasulla, Natalia Gorojovsky, Gustavo E. Gudesblat, Maria Georgina Herrera, L. I. Ibañez, Tommy Idrovo, Matías Iglesias Randon, L. Kamenetzky, A. Nadra, D. Noseda, C. Pavan, María F. Pavan, M. F. Pignataro, Ernesto A. Román, L. Ruberto, N. Rubinstein, Javier Santos, F. Duarte, A. Zelada
Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden
Wollborn, David, Munkler, Lara Pauline, Horstmann, Rebekka, Germer, Andrea, Blank, Lars M., Büchs, Jochen · 2022
Full title: Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indicator of metabolic burden
Authors: Wollborn, David, Munkler, Lara Pauline, Horstmann, Rebekka, Germer, Andrea, Blank, Lars M., Büchs, Jochen
Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement.
Full title: Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air pressure instead of pure oxygen supplement.
Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris.
Full title: Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris.
Development of a Pichia pastoris whole-cell biocatalyst with overexpression of mutant lipase I PCLG47I from Penicillium cyclopium for biodiesel production.
Full title: Development of a Pichia pastoris whole-cell biocatalyst with overexpression of mutant lipase I PCLG47I from Penicillium cyclopium for biodiesel production.
Expression of <sup>2</sup>H, <sup>13</sup>C, <sup>15</sup>N-labeled NIST-Fab fragment in the methylotrophic yeast <i>Komagataella phaffii</i> for nuclear magnetic resonance studies
Chao, Kinlin, O’Dell, William B., Solomon, Tsega L., Brinson, Robert G., Marino, John P., Kelman, Zvi · 2023
Full title: Expression of <sup>2</sup>H, <sup>13</sup>C, <sup>15</sup>N-labeled NIST-Fab fragment in the methylotrophic yeast <i>Komagataella phaffii</i> for nuclear magnetic resonance studies
Authors: Chao, Kinlin, O’Dell, William B., Solomon, Tsega L., Brinson, Robert G., Marino, John P., Kelman, Zvi
A combined flow injection/reversed phase chromatography – high resolution mass spectrometry workflow for accurate absolute lipid quantification with <sup>13</sup> C- internal standards
Full title: A combined flow injection/reversed phase chromatography – high resolution mass spectrometry workflow for accurate absolute lipid quantification with <sup>13</sup> C- internal standards
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher · 2020
Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Authors: Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher
Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters
Dalvie, Neil C., Rodriguez‐Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Célia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dong Soo, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanné, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith M., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice, Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher · 2021
Full title: Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamsters
Authors: Dalvie, Neil C., Rodriguez‐Aponte, Sergio A., Hartwell, Brittany L., Tostanoski, Lisa H., Biedermann, Andrew M., Crowell, Laura E., Kaur, Kawaljit, Kumru, Ozan S., Carter, Lauren, Yu, Jingyou, Chang, Aiquan, McMahan, Katherine, Courant, Thomas, Lebas, Célia, Lemnios, Ashley A., Rodrigues, Kristen A., Silva, Murillo, Johnston, Ryan S., Naranjo, Christopher A., Tracey, Mary Kate, Brady, Joseph R., Whittaker, Charles A., Yun, Dong Soo, Kar, Swagata, Porto, Maciel, Lok, Megan, Andersen, Hanné, Lewis, Mark G., Love, Kerry R., Camp, Danielle L., Silverman, Judith M., Kleanthous, Harry, Joshi, Sangeeta B., Volkin, David B., Dubois, Patrice, Collin, Nicolas, King, Neil P., Barouch, Dan H., Irvine, Darrell J., Love, J. Christopher
Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii.
Dalvie, Neil C, Biedermann, Andrew M, Rodriguez-Aponte, Sergio A, Naranjo, Christopher A, Rao, Harish D, Rajurkar, Meghraj P, Lothe, Rakesh R, Shaligram, Umesh S, Johnston, Ryan S, Crowell, Laura E, Castelino, Seraphin, Tracey, Mary Kate, Whittaker, Charles A, Love, J Christopher · 2021
Full title: Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii.
Authors: Dalvie, Neil C, Biedermann, Andrew M, Rodriguez-Aponte, Sergio A, Naranjo, Christopher A, Rao, Harish D, Rajurkar, Meghraj P, Lothe, Rakesh R, Shaligram, Umesh S, Johnston, Ryan S, Crowell, Laura E, Castelino, Seraphin, Tracey, Mary Kate, Whittaker, Charles A, Love, J Christopher
P <sub> <i>AOX1</i> </sub> expression in mixed-substrate continuous cultures of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) is completely determined by methanol consumption regardless of the secondary carbon source
Singh, Anamika, Narang, Atul · 2021
Full title: P <sub> <i>AOX1</i> </sub> expression in mixed-substrate continuous cultures of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) is completely determined by methanol consumption regardless of the secondary carbon source
Steric accessibility of the <i>N</i> -terminus improves the titer and quality of recombinant proteins secreted from <i>Komagataella phaffii</i>
Dalvie, Neil C., Naranjo, Christopher A., Rodriguez‐Aponte, Sergio A., Johnston, Ryan S., Love, J. Christopher · 2022
Full title: Steric accessibility of the <i>N</i> -terminus improves the titer and quality of recombinant proteins secreted from <i>Komagataella phaffii</i>
Authors: Dalvie, Neil C., Naranjo, Christopher A., Rodriguez‐Aponte, Sergio A., Johnston, Ryan S., Love, J. Christopher
Flux balance analysis predicts NADP phosphatase and NADH kinase are critical to balancing redox during xylose fermentation in <i>Scheffersomyces stipitis</i>
Correia, Kevin, Khusnutdinova, Anna N., Li, Peter Yan, Joo, Jeong Chan, Brown, Greg, Yakunin, Alexander F., Mahadevan, Radhakrishnan · 2018
Full title: Flux balance analysis predicts NADP phosphatase and NADH kinase are critical to balancing redox during xylose fermentation in <i>Scheffersomyces stipitis</i>
Authors: Correia, Kevin, Khusnutdinova, Anna N., Li, Peter Yan, Joo, Jeong Chan, Brown, Greg, Yakunin, Alexander F., Mahadevan, Radhakrishnan
The Mut <sup>+</sup> strain of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) expresses P <i> <sub>AOX1</sub> </i> 5 and 10 times faster than Mut <sup>s</sup> and Mut <sup>−</sup> strains: Evidence that formaldehyde or/and formate are true inducers of AOX
Singh, Anamika, Narang, Atul · 2019
Full title: The Mut <sup>+</sup> strain of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) expresses P <i> <sub>AOX1</sub> </i> 5 and 10 times faster than Mut <sup>s</sup> and Mut <sup>−</sup> strains: Evidence that formaldehyde or/and formate are true inducers of AOX
Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1
Ravin, Nikolai V., Eldarov, Michael A., Kadnikov, Vitaly V., Beletsky, Alexey V., Schneider, Jessica, Mardanova, Eugenia S., Smekalova, Elena M., Zvereva, Maria I., Донцова, О. А., Mardanov, Andrey V., Skryabin, K. G. · 2013
Full title: Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1
Authors: Ravin, Nikolai V., Eldarov, Michael A., Kadnikov, Vitaly V., Beletsky, Alexey V., Schneider, Jessica, Mardanova, Eugenia S., Smekalova, Elena M., Zvereva, Maria I., Донцова, О. А., Mardanov, Andrey V., Skryabin, K. G.
Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase.
Hemmerich, Johannes, Adelantado, Núria, Barrigón, José Manuel, Ponte, Xavier, Hörmann, Astrid, Ferrer, Pau, Kensy, Frank, Valero, Francisco · 2014
Full title: Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase.
Authors: Hemmerich, Johannes, Adelantado, Núria, Barrigón, José Manuel, Ponte, Xavier, Hörmann, Astrid, Ferrer, Pau, Kensy, Frank, Valero, Francisco
Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime.
Holmes, William J, Darby, Richard Aj, Wilks, Martin Db, Smith, Rodney, Bill, Roslyn M · 2009
Full title: Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime.
Authors: Holmes, William J, Darby, Richard Aj, Wilks, Martin Db, Smith, Rodney, Bill, Roslyn M
Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel.
Full title: Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel.
Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level
Prielhofer, Roland, Cartwright, Stephanie P., Graf, Alexandra B., Valli, Minoska, Bill, Roslyn M., Mattanovich, Diethard, Gasser, Brigitte · 2015
Full title: Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level
Authors: Prielhofer, Roland, Cartwright, Stephanie P., Graf, Alexandra B., Valli, Minoska, Bill, Roslyn M., Mattanovich, Diethard, Gasser, Brigitte
Comparative genomics and transcriptomics of Pichia pastoris
Love, Kerry R., Shah, Kartik, Whittaker, Charles A., Wu, Jie, Bartlett, M. Catherine, Ma, Duanduan, Leeson, Rachel, Priest, Margaret, Borowsky, Jonathan, Young, Sarah, Love, J. Christopher · 2016
Full title: Comparative genomics and transcriptomics of Pichia pastoris
Authors: Love, Kerry R., Shah, Kartik, Whittaker, Charles A., Wu, Jie, Bartlett, M. Catherine, Ma, Duanduan, Leeson, Rachel, Priest, Margaret, Borowsky, Jonathan, Young, Sarah, Love, J. Christopher
Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33.
Full title: Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33.
An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)
Che, Zhiqun, Cao, Xiaoyan, Chen, Guiguang, Liang, Zhiqun · 2020
Full title: An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)
Authors: Che, Zhiqun, Cao, Xiaoyan, Chen, Guiguang, Liang, Zhiqun
Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.
Moser, Josef W, Prielhofer, Roland, Gerner, Samuel M, Graf, Alexandra B, Wilson, Iain B H, Mattanovich, Diethard, Dragosits, Martin · 2017
Full title: Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.
Authors: Moser, Josef W, Prielhofer, Roland, Gerner, Samuel M, Graf, Alexandra B, Wilson, Iain B H, Mattanovich, Diethard, Dragosits, Martin
Full title: Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris
Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage
Garrigós‐Martínez, Javier, Nieto‐Taype, Miguel Angel, Gasset-Franch, Arnau, Montesinos, José Luis González, Garcia‐Ortega, Xavier, Valero, Francisco · 2019
Full title: Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage
Authors: Garrigós‐Martínez, Javier, Nieto‐Taype, Miguel Angel, Gasset-Franch, Arnau, Montesinos, José Luis González, Garcia‐Ortega, Xavier, Valero, Francisco
Full title: Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher · 2021
Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Authors: Dalvie, Neil C., Brady, Joseph R., Crowell, Laura E., Tracey, Mary Kate, Biedermann, Andrew M., Kaur, Kawaljit, Hickey, John M., Kristensen, D. Lee, Bonnyman, Alexandra, Rodriguez‐Aponte, Sergio A., Whittaker, Charles A., Bok, Marina, Vega, Celina G., Mukhopadhyay, Tarit, Joshi, Sangeeta B., Volkin, David B., Parreño, Viviana, Love, Kerry R., Love, J. Christopher
High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux
Full title: High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux
Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications
Full title: Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitness and functionality for bioproduction applications
Full title: Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii
Chaetomella raphigera β-glucosidase D2-BGL has intriguing structural features and a high substrate affinity that renders it an efficient cellulase supplement for lignocellulosic biomass hydrolysis.
Kao, Mu-Rong, Kuo, Hsion-Wen, Lee, Cheng-Chung, Huang, Kuan-Ying, Huang, Ting-Yen, Li, Chen-Wei, Chen, C Will, Wang, Andrew H-J, Yu, Su-May, Ho, Tuan-Hua David · 2019
Full title: Chaetomella raphigera β-glucosidase D2-BGL has intriguing structural features and a high substrate affinity that renders it an efficient cellulase supplement for lignocellulosic biomass hydrolysis.
Authors: Kao, Mu-Rong, Kuo, Hsion-Wen, Lee, Cheng-Chung, Huang, Kuan-Ying, Huang, Ting-Yen, Li, Chen-Wei, Chen, C Will, Wang, Andrew H-J, Yu, Su-May, Ho, Tuan-Hua David
Enhanced Co-culture System Using <i>Escherichia coli</i> and <i>Pichia pastoris</i> (<i>Komagataella phaffii</i>) for Improved Microbial Production of Valuable Plant Alkaloids
Full title: Enhanced Co-culture System Using <i>Escherichia coli</i> and <i>Pichia pastoris</i> (<i>Komagataella phaffii</i>) for Improved Microbial Production of Valuable Plant Alkaloids
Structure of SALO, a leishmaniasis vaccine candidate from the sand fly Lutzomyia longipalpis.
Asojo, Oluwatoyin A, Kelleher, Alan, Liu, Zhuyun, Pollet, Jeroen, Hudspeth, Elissa M, Rezende, Wanderson C, Groen, Mallory Jo, Seid, Christopher A, Abdeladhim, Maha, Townsend, Shannon, de Castro, Waldione, Mendes-Sousa, Antonio, Bartholomeu, Daniella Castanheira, Fujiwara, Ricardo Toshio, Bottazzi, Maria Elena, Hotez, Peter J, Zhan, Bin, Oliveira, Fabiano, Kamhawi, Shaden, Valenzuela, Jesus G · 2017
Full title: Structure of SALO, a leishmaniasis vaccine candidate from the sand fly Lutzomyia longipalpis.
Authors: Asojo, Oluwatoyin A, Kelleher, Alan, Liu, Zhuyun, Pollet, Jeroen, Hudspeth, Elissa M, Rezende, Wanderson C, Groen, Mallory Jo, Seid, Christopher A, Abdeladhim, Maha, Townsend, Shannon, de Castro, Waldione, Mendes-Sousa, Antonio, Bartholomeu, Daniella Castanheira, Fujiwara, Ricardo Toshio, Bottazzi, Maria Elena, Hotez, Peter J, Zhan, Bin, Oliveira, Fabiano, Kamhawi, Shaden, Valenzuela, Jesus G
Conformational and thermal stability improvements for the large-scale production of yeast-derived rabbit hemorrhagic disease virus-like particles as multipurpose vaccine.
Fernández, Erlinda, Toledo, Jorge R, Méndez, Lídice, González, Nemecio, Parra, Francisco, Martín-Alonso, José M, Limonta, Miladys, Sánchez, Kosara, Cabrales, Ania, Estrada, Mario P, Rodríguez-Mallón, Alina, Farnós, Omar · 2013
Full title: Conformational and thermal stability improvements for the large-scale production of yeast-derived rabbit hemorrhagic disease virus-like particles as multipurpose vaccine.
Authors: Fernández, Erlinda, Toledo, Jorge R, Méndez, Lídice, González, Nemecio, Parra, Francisco, Martín-Alonso, José M, Limonta, Miladys, Sánchez, Kosara, Cabrales, Ania, Estrada, Mario P, Rodríguez-Mallón, Alina, Farnós, Omar
Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
Edwards-Jones, Bryn, Aw, Rochelle, Barton, Geraint, Tredwell, Gregory D., Bundy, Jacob G., Leak, David J. · 2015
Full title: Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
Authors: Edwards-Jones, Bryn, Aw, Rochelle, Barton, Geraint, Tredwell, Gregory D., Bundy, Jacob G., Leak, David J.
Integration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism
Tomàs‐Gamisans, Màrius, Ferrer, Pau, Albiol, Joan · 2016
Full title: Integration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Glycosylation Pathways, Lipid and Energy Metabolism
Authors: Tomàs‐Gamisans, Màrius, Ferrer, Pau, Albiol, Joan
Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants
Detering, Thorben, Mundry, Katharina, Berger, Ralf G. · 2020
Full title: Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants
Authors: Detering, Thorben, Mundry, Katharina, Berger, Ralf G.
Population genomics shows no distinction between pathogenic Candida krusei and environmental Pichia kudriavzevii: One species, four names
Douglass, Alexander, Offei, Benjamin, Braun‐Galleani, Stephanie, Coughlan, Aisling Y., Martos, Alexandre A. R., Ortiz‐Merino, Raúl A., Byrne, Kevin P., Wolfe, Kenneth H. · 2018
Full title: Population genomics shows no distinction between pathogenic Candida krusei and environmental Pichia kudriavzevii: One species, four names
Authors: Douglass, Alexander, Offei, Benjamin, Braun‐Galleani, Stephanie, Coughlan, Aisling Y., Martos, Alexandre A. R., Ortiz‐Merino, Raúl A., Byrne, Kevin P., Wolfe, Kenneth H.
Constitutive High Expression Level of A Synthetic Deleted Encoding Gene of <i>Talaromyces minioluteus</i> Endodextranase Variant (r-TmDEX49A-ΔSP-ΔN30) in <i>Komagataella phaffii</i> (<i>Pichia pastoris</i>)
Full title: Constitutive High Expression Level of A Synthetic Deleted Encoding Gene of <i>Talaromyces minioluteus</i> Endodextranase Variant (r-TmDEX49A-ΔSP-ΔN30) in <i>Komagataella phaffii</i> (<i>Pichia pastoris</i>)
Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain (RBD) Using Pichia pastoris in Stirred-Tank Bioreactor
Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, María Florencia, Wetzler, Diana E., Gentilli, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas AM · 2023
Full title: Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain (RBD) Using Pichia pastoris in Stirred-Tank Bioreactor
Authors: Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, María Florencia, Wetzler, Diana E., Gentilli, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas AM
Full title: Production of Plant-Based Meat Flavor Compound – Leghemo-Globin (LegH) in Recombinant <em>Komagataella phaffii</em> Fed-Batch Fermentations
State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
Full title: State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
Establishing site-specific recombination mediated cassette exchange in Pichia pastoris and development of an optimized process for the production of mouse Tmprss2
Schinkowski, Christian · 2017
Full title: Establishing site-specific recombination mediated cassette exchange in Pichia pastoris and development of an optimized process for the production of mouse Tmprss2
Overall Key Performance Indicator to Optimizing Operation of High-Pressure Homogenizers for a Reliable Quantification of Intracellular Components in Pichia pastoris.
Garcia-Ortega, Xavier, Reyes, Cecilia, Montesinos, José Luis, Valero, Francisco · 2015
Full title: Overall Key Performance Indicator to Optimizing Operation of High-Pressure Homogenizers for a Reliable Quantification of Intracellular Components in Pichia pastoris.
Authors: Garcia-Ortega, Xavier, Reyes, Cecilia, Montesinos, José Luis, Valero, Francisco
Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
Gasset, Arnau, Garcia‐Ortega, Xavier, Garrigós‐Martínez, Javier, Valero, Francisco, Montesinos, José Luis González · 2022
Full title: Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
Authors: Gasset, Arnau, Garcia‐Ortega, Xavier, Garrigós‐Martínez, Javier, Valero, Francisco, Montesinos, José Luis González
Full title: Enantioselective Biosynthesis of L-Phenyllactic Acid From Phenylpyruvic Acid In Vitro by L-Lactate Dehydrogenase Coupling With Glucose Dehydrogenase.
PAOX1 expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determined by methanol consumption regardless of the secondary carbon source
Singh, Anamika, Narang, Atul · 2023
Full title: PAOX1 expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determined by methanol consumption regardless of the secondary carbon source
Full title: Comparative transcriptomic analysis-based identification of the regulation of foreign proteins with different stabilities expressed in Pichia pastoris
Multiple strategies were adopted to optimize the enzymatic characteristics and improve the expression of bovine chymosin BtChy in Kluyveromyces lactis for cheese production
Full title: Multiple strategies were adopted to optimize the enzymatic characteristics and improve the expression of bovine chymosin BtChy in Kluyveromyces lactis for cheese production
From linoleic acid to hexanal and hexanol by whole cell catalysis with a lipoxygenase, hydroperoxide lyase and reductase cascade in Komagataella phaffii
Hashem, Chiam, Hochrinner, Julius, Bürgler, Moritz B., Rinnofner, Claudia, Pichler, Harald, Winkler, Margit · 2022
Full title: From linoleic acid to hexanal and hexanol by whole cell catalysis with a lipoxygenase, hydroperoxide lyase and reductase cascade in Komagataella phaffii
Authors: Hashem, Chiam, Hochrinner, Julius, Bürgler, Moritz B., Rinnofner, Claudia, Pichler, Harald, Winkler, Margit
Boosting Expression of a Specifically Targeted Antimicrobial Peptide K in Pichia pastoris by Employing a 2A Self-Cleaving Peptide-Based Expression System.
Full title: Boosting Expression of a Specifically Targeted Antimicrobial Peptide K in Pichia pastoris by Employing a 2A Self-Cleaving Peptide-Based Expression System.
Effect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineered for Lactic Acid Production
Melo, Nadiele Tamires Moreira, Mulder, Kelly C.L., Nicola, André Moraes, Carvalho, Lucas Silva, Menino, Gisele, Mulinari, Eduardo, Parachin, Nádia Skorupa · 2018
Full title: Effect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineered for Lactic Acid Production
Authors: Melo, Nadiele Tamires Moreira, Mulder, Kelly C.L., Nicola, André Moraes, Carvalho, Lucas Silva, Menino, Gisele, Mulinari, Eduardo, Parachin, Nádia Skorupa
Full title: Preparative Production of Functionalized (N- and O-Heterocyclic) Polycyclic Aromatic Hydrocarbons by Human Cytochrome P450 3A4 in a Bioreactor
Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris
Maity, Nitu, Mishra, Saroj · 2019
Full title: Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris
Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi.
Birchfield, Aaron S, McIntosh, Cecilia A · 2024
Full title: Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase from Citrus paradisi.
Full title: Performance of Recombinant Komagataella phaffii in Plant-Based Meat Flavor Compound-Leghemoglobin (LegH) Production through Fed-Batch Fermentations
State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
Full title: State of the Art Technologies for High Yield Heterologous Expression and Production of Oxidoreductase Enzymes: Glucose Oxidase, Cellobiose Dehydrogenase, Horseradish Peroxidase, and Laccases in Yeasts P. pastoris and S. cerevisiae
Enhancing Antimicrobial Peptide Productivity in Pichia pastoris (Muts Strain) by Improving the Fermentation Process Based on Increasing the Volumetric Methanol Consumption Rate
Full title: Enhancing Antimicrobial Peptide Productivity in Pichia pastoris (Muts Strain) by Improving the Fermentation Process Based on Increasing the Volumetric Methanol Consumption Rate
Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain Using Pichia pastoris in Stirred-Tank Bioreactor
Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, Florencia, Wetzler, Diana E., Gentili, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas · 2023
Full title: Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain Using Pichia pastoris in Stirred-Tank Bioreactor
Authors: Noseda, Diego G., D’Alessio, Cecilia, Santos, Javier, Idrovo-Hidalgo, Tommy, Pignataro, Florencia, Wetzler, Diana E., Gentili, Hernán, Nadra, Alejandro D., Román, Ernesto A., Paván, Carlos, Ruberto, Lucas
Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3
Full title: Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pastoris Producing Hirudin Variant 3
Heterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
Zadorozhny, A. V., Ushakov, Viktor Sergeevich, Розанов, А. С., Bogacheva, Natalia Vladimirovna, Shlyakhtun, Valeria Nikolayevna, Voskoboev, Mikhail Evgenyevich, Korzhuk, Anton V., Romancev, Vladislav Anatolevich, Bannikova, Svetlana V., Mescheryakova, Irina Anatolyevna, Антонов, Е. В., Vasilieva, Asya R., Pavlova, Elena Iurevna, Chesnokov, Danil Olegovich, Shedko, Elizaveta Dmitrievna, Bryanskaya, A. V., Bochkov, D. V., Goryachkovskaya, T. N., Peltek, S. E. · 2022
Full title: Heterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
Authors: Zadorozhny, A. V., Ushakov, Viktor Sergeevich, Розанов, А. С., Bogacheva, Natalia Vladimirovna, Shlyakhtun, Valeria Nikolayevna, Voskoboev, Mikhail Evgenyevich, Korzhuk, Anton V., Romancev, Vladislav Anatolevich, Bannikova, Svetlana V., Mescheryakova, Irina Anatolyevna, Антонов, Е. В., Vasilieva, Asya R., Pavlova, Elena Iurevna, Chesnokov, Danil Olegovich, Shedko, Elizaveta Dmitrievna, Bryanskaya, A. V., Bochkov, D. V., Goryachkovskaya, T. N., Peltek, S. E.
Production of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unveiling Antimicrobial and Anticancer Functionalities.
Full title: Production of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unveiling Antimicrobial and Anticancer Functionalities.
Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Хлебодарова, Т. М., Bogacheva, N. V., Zadorozhny, A. V., Bryanskaya, A. V., Vasilieva, Asya R., Chesnokov, Danil Olegovich, Pavlova, Elena I., Peltek, S. E. · 2024
Full title: Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Authors: Хлебодарова, Т. М., Bogacheva, N. V., Zadorozhny, A. V., Bryanskaya, A. V., Vasilieva, Asya R., Chesnokov, Danil Olegovich, Pavlova, Elena I., Peltek, S. E.
Recombinant Inga Laurina Trypsin Inhibitor (ILTI) Production in Komagataella Phaffii Confirms Its Potential Anti-Biofilm Effect and Reveals an Anti-Tumoral Activity.
Carneiro, Fábio C, Weber, Simone S, Silva, Osmar N, Jacobowski, Ana Cristina, Ramada, Marcelo H S, Macedo, Maria L R, Franco, Octávio L, Parachin, Nádia S · 2018
Full title: Recombinant Inga Laurina Trypsin Inhibitor (ILTI) Production in Komagataella Phaffii Confirms Its Potential Anti-Biofilm Effect and Reveals an Anti-Tumoral Activity.
Authors: Carneiro, Fábio C, Weber, Simone S, Silva, Osmar N, Jacobowski, Ana Cristina, Ramada, Marcelo H S, Macedo, Maria L R, Franco, Octávio L, Parachin, Nádia S
Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Komagataella phaffii Strains Utilizing Glycerol as Substrate
Melo, Nadielle Tamires Moreira, Pontes, Gabriela Coimbra, Procópio, Dielle Pierotti, Cunha, Gabriel Caetano de Góis e, Eliodório, Kevy Pontes, Paes, Hugo Costa, Basso, Thiago Olitta, Parachin, Nádia Skorupa · 2020
Full title: Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Komagataella phaffii Strains Utilizing Glycerol as Substrate
Authors: Melo, Nadielle Tamires Moreira, Pontes, Gabriela Coimbra, Procópio, Dielle Pierotti, Cunha, Gabriel Caetano de Góis e, Eliodório, Kevy Pontes, Paes, Hugo Costa, Basso, Thiago Olitta, Parachin, Nádia Skorupa
Metodologia para projeto de biorreatores industriais via otimização multiobjetivo com base em parâmetros de desempenho calculados por técnicas de CFD
Seleghim Junior, Paulo · 2015
Full title: Metodologia para projeto de biorreatores industriais via otimização multiobjetivo com base em parâmetros de desempenho calculados por técnicas de CFD
Evaluation of product distribution in chemostat and batch fermentation in lactic acid-producing komagataella phaffii strains utilizing glycerol as substrate
Parachin, Nádia Skorupa · 2020
Full title: Evaluation of product distribution in chemostat and batch fermentation in lactic acid-producing komagataella phaffii strains utilizing glycerol as substrate
Simulation and techno-economic analysis of production processes of novel generation L-Asparaginases using recombinant Escherichia coli and Pichia pastoris
Azzoni, Adriano Rodrigues · 2022
Full title: Simulation and techno-economic analysis of production processes of novel generation L-Asparaginases using recombinant Escherichia coli and Pichia pastoris
High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
Blanchard, Véronique; Gadkari, R.P; George, A.V.E. et al. · 2008
Full title: High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Dalvie, Neil C; Brady, Joseph R; Crowell, Laura E et al. · 2021
Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus
Authors: Dalvie, Neil C; Brady, Joseph R; Crowell, Laura E; Tracey, Mary K; Biedermann, Andrew M; Kaur, Kawaljit; Hickey, John M; Kristensen, D. L; Bonnyman, Alexandra D; Rodriguez-Aponte, Sergio A; Whittaker, Charles A; Bok, Marina; Vega, Celina; Mukhopadhyay, Tarit K; Joshi, Sangeeta B; Volkin, David B
Optimizing recombinant protein production in Chlamydomonas reinhardtii: turning a model organism into a commercially relevant biotechnological host
Torres-Tiji, Yasin · 2021
Full title: Optimizing recombinant protein production in Chlamydomonas reinhardtii: turning a model organism into a commercially relevant biotechnological host
Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
Manderson, Daniel · 2017
Full title: Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University
Application of alternative fermenter design in whey-ethanol production (a preliminary study) : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Biotechnology at Massey University
Tin, Calvin Siu Fan · 2016
Full title: Application of alternative fermenter design in whey-ethanol production (a preliminary study) : a thesis presented in partial fulfilment of the requirements for the degree of Master of Technology in Biotechnology at Massey University
Estudio de la dispersión multifásica y área interfacial de transferencia de masa a nivel local en un sistema modelo de fermentación micelial de tres fases
Holguín Salas, Alehlí · 2023
Full title: Estudio de la dispersión multifásica y área interfacial de transferencia de masa a nivel local en un sistema modelo de fermentación micelial de tres fases
Kinetic study of fed-batch and continuous bioconversion processes for L-Phenylacetylcarbinol (L-PAC) production by the yeast Candida utilis
Wang, Bin · 1993
Full title: Kinetic study of fed-batch and continuous bioconversion processes for L-Phenylacetylcarbinol (L-PAC) production by the yeast Candida utilis
Model-based real-time optimisation of a fed-batch cyanobacterial hydrogen production process using economic model predictive control strategy
del Rio-Chanona, Ehecatl Antonio; Zhang, Dongda; Vassiliadis, Vassilios S · 2015
Full title: Model-based real-time optimisation of a fed-batch cyanobacterial hydrogen production process using economic model predictive control strategy
Authors: del Rio-Chanona, Ehecatl Antonio; Zhang, Dongda; Vassiliadis, Vassilios S
Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter
Barrigón de San Marcos, José Manuel · 2015
Full title: Model-based design and development of operational strategies for Rhizopus oryzae lipase production in Pichia pastoris under the AOX1 promoter
Utilización de glicerol proveniente de la industria de biodiesel como fuente de carbono para la producción de lipasas recombinantes en Pichia pastoris
Loango Chamorro, Nelsy · 2015
Full title: Utilización de glicerol proveniente de la industria de biodiesel como fuente de carbono para la producción de lipasas recombinantes en Pichia pastoris
A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches
Garcia Ortega, Xavier · 2016
Full title: A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches
L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic
Cámara Rey, Elena · 2017
Full title: L’increment de la dosi gènica com a estratègia de millora de la producció de proteïnes recombinants al llevat Pichia pastoris: revertint les limitacions genètiques del sistema biològic
Estratègies d’operació per a la producció de lipases de Rhizopus oryzae amb el fenotip Mut(s) de Pichia pastoris mitjançant substrats mixtes
Arnau Jiménez, Carol · 2011
Full title: Estratègies d’operació per a la producció de lipases de Rhizopus oryzae amb el fenotip Mut(s) de Pichia pastoris mitjançant substrats mixtes
Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris
Nieto Taype, Miguel Angel · 2020
Full title: Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris
Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris
Garrigós Martínez, Javier · 2021
Full title: Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris
Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess
Gasset Franch, Arnau · 2024
Full title: Physiological control of Pichia pastoris for the production of recombinant proteins: A novel Respiratory Quotient control based on Artificial Intelligence and scale-up of the bioprocess