Ph Optimization And Its Effects On Protein Quality

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1. Documents

1 paper 25 patents 35 theses

Full text available

Thesis Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso

Russo, Elisa Maria de Sousa · 2017 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso

Authors: Russo, Elisa Maria de Sousa

Year: 2017

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://www.teses.usp.br/teses/disponiveis/60/60135/tde-23112017-101039/

Thesis Exploring the role of a glycosylated L-asparaginase expressed by a recombinant Pichia pastoris as an antileukemic biopharmaceutical

Pessoa Junior, Adalberto · 2021 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Exploring the role of a glycosylated L-asparaginase expressed by a recombinant Pichia pastoris as an antileukemic biopharmaceutical

Authors: Pessoa Junior, Adalberto

Year: 2021

Venue: Universidade de São Paulo – USP Digital Library

DOI: https://doi.org/10.11606/T.9.2021.tde-21122021-144906

Thesis Design of a cultivation medium for protein production in Pichia pastoris based on genome-wide biological understanding

Matthews, Catherine Bartlett. · 2019 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Design of a cultivation medium for protein production in Pichia pastoris based on genome-wide biological understanding

Authors: Matthews, Catherine Bartlett.

Year: 2019

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/122528

Thesis Molecular, Genetic, and Process Approaches for Improving Secreted Pharmaceutical Protein Quality in Komagataella phaffii

Yang, Yuchen · 2024 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Molecular, Genetic, and Process Approaches for Improving Secreted Pharmaceutical Protein Quality in Komagataella phaffii

Authors: Yang, Yuchen

Year: 2024

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/157251

Thesis The development and application of mass-spectrometry-based tools to monitor proteome remodeling in microbes

Telusma, Bertina · 2024 · Massachusetts Institute of Technology (MIT) · DOI

Full title: The development and application of mass-spectrometry-based tools to monitor proteome remodeling in microbes

Authors: Telusma, Bertina

Year: 2024

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/157155

Thesis Enhanced Production of Heterologous Protein by Recombinant Aspergillus niger Through Bioprocessing Strategies in Submerged Culture

Wang, Liping · 2002 · OhioLINK Electronic Theses and Dissertations · DOI

Full title: Enhanced Production of Heterologous Protein by Recombinant Aspergillus niger Through Bioprocessing Strategies in Submerged Culture

Authors: Wang, Liping

Year: 2002

Venue: OhioLINK Electronic Theses and Dissertations

DOI: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1038425153

Thesis Produção de glicerol quinase em Pichia pastoris

Aizemberg, Raquel [UNESP] · 2014 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Produção de glicerol quinase em Pichia pastoris

Authors: Aizemberg, Raquel [UNESP]

Year: 2014

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://hdl.handle.net/11449/88349

Thesis Enhanced biocatalyst production for(R)-phenylacetylcarbinol synthesis

Chen, Allen Kuan-Liang · 2006 · UNSW Sydney · DOI

Full title: Enhanced biocatalyst production for(R)-phenylacetylcarbinol synthesis

Authors: Chen, Allen Kuan-Liang

Year: 2006

Venue: UNSW Sydney

DOI: http://hdl.handle.net/1959.4/32825

Thesis Optimization of the production of recombinant macrophage inhibitory cytokine 1 (MIC-1) from Pichia pastoris

Pratanaphon, Ronachai · 2006 · UNSW Sydney · DOI

Full title: Optimization of the production of recombinant macrophage inhibitory cytokine 1 (MIC-1) from Pichia pastoris

Authors: Pratanaphon, Ronachai

Year: 2006

Venue: UNSW Sydney

DOI: http://hdl.handle.net/1959.4/68401

Thesis A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches

Garcia Ortega, Xavier · 2016 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches

Authors: Garcia Ortega, Xavier

Year: 2016

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/384531

Thesis Development of the production process for a recombinant hormone and evaluation of its biological activity

Font Ingles, Albert · 2013 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Development of the production process for a recombinant hormone and evaluation of its biological activity

Authors: Font Ingles, Albert

Year: 2013

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/123298

Thesis Development of Pichia pastoris for the production of human α-galactosidase A as a model lysosomal enzyme

Barreiro Vázquez, Antonio · 2016 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Development of Pichia pastoris for the production of human α-galactosidase A as a model lysosomal enzyme

Authors: Barreiro Vázquez, Antonio

Year: 2016

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/382822

Thesis Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris

Garrigós Martínez, Javier · 2021 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris

Authors: Garrigós Martínez, Javier

Year: 2021

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/671119

Thesis Overcoming the secretory limitations in Pichia pastoris for recombinant protein production

Barrero Peña, Juan José · 2021 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Overcoming the secretory limitations in Pichia pastoris for recombinant protein production

Authors: Barrero Peña, Juan José

Year: 2021

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/670387

Thesis Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris

Nieto Taype, Miguel Angel · 2020 · Universitat Autònoma de Barcelona (TDX) · DOI

Full title: Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris

Authors: Nieto Taype, Miguel Angel

Year: 2020

Venue: Universitat Autònoma de Barcelona (TDX)

DOI: http://hdl.handle.net/10803/670163

Thesis Characterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris

Blanchard, V.; Gadkari, R.A.; Gerwig, G.J. et al. · 2007 · Utrecht University · DOI

Full title: Characterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris

Authors: Blanchard, V.; Gadkari, R.A.; Gerwig, G.J.; Leeflang, B.R.; Dighe, R.R.; Kamerling, J.P.

Year: 2007

Venue: Utrecht University

DOI: https://dspace.library.uu.nl/handle/1874/27466

Thesis A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

Lee, Kevin S.; Lee, Harry L. T.; Mozdzierz, Nicholas Joseph et al. · 2017 · Massachusetts Institute of Technology (MIT) · DOI

Full title: A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

Authors: Lee, Kevin S.; Lee, Harry L. T.; Mozdzierz, Nicholas Joseph; Love, Kerry R.; Shah, Kartik A; Ram, Rajeev J; Love, John C

Year: 2017

Venue: Massachusetts Institute of Technology (MIT)

DOI: http://hdl.handle.net/1721.1/109453

Thesis Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

Dalvie, Neil C; Brady, Joseph R; Crowell, Laura E et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

Authors: Dalvie, Neil C; Brady, Joseph R; Crowell, Laura E; Tracey, Mary K; Biedermann, Andrew M; Kaur, Kawaljit; Hickey, John M; Kristensen, D. L; Bonnyman, Alexandra D; Rodriguez-Aponte, Sergio A; Whittaker, Charles A; Bok, Marina; Vega, Celina; Mukhopadhyay, Tarit K; Joshi, Sangeeta B; Volkin, David B

Year: 2021

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/136780

Thesis Development of a platform process for the production and purification of single‐domain antibodies

Crowell, Laura E; Goodwine, Chaz; Holt, Carla S et al. · 2021 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Development of a platform process for the production and purification of single‐domain antibodies

Authors: Crowell, Laura E; Goodwine, Chaz; Holt, Carla S; Rocha, Lucia; Vega, Celina; Rodriguez, Sergio A; Dalvie, Neil C; Tracey, Mary K; Puntel, Mariana; Wigdorovitz, Andrés; Parreño, Viviana; Love, Kerry R; Cramer, Steven M; Love, J Christopher

Year: 2021

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/135524

Thesis Accelerating cell culture media development using Bayesian optimization-based iterative experimental design

Narayanan, Harini; Hinckley, Joshua A; Barry, Rachel et al. · 2025 · Massachusetts Institute of Technology (MIT) · DOI

Full title: Accelerating cell culture media development using Bayesian optimization-based iterative experimental design

Authors: Narayanan, Harini; Hinckley, Joshua A; Barry, Rachel; Dang, Brendan; Wolffe, Lenna A; Atari, Adel; Tseng, Yuen-Yi; Love, J Christopher

Year: 2025

Venue: Massachusetts Institute of Technology (MIT)

DOI: https://hdl.handle.net/1721.1/163220

Thesis Optimizing Expression and Purification of HaTRPA1

Hansson Wennersten, Wilhelm · 2024 · Lund University Student Papers · DOI

Full title: Optimizing Expression and Purification of HaTRPA1

Authors: Hansson Wennersten, Wilhelm

Year: 2024

Venue: Lund University Student Papers

DOI: http://lup.lub.lu.se/student-papers/record/9159056

Thesis Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University

Manderson, Daniel · 2017 · Massey University · DOI

Full title: Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University

Authors: Manderson, Daniel

Year: 2017

Venue: Massey University

DOI: http://hdl.handle.net/10179/12019

Thesis Production of thermostable L2 lipase using recombinant Pichia pastoris

Shahidan, Noor Hidayah · 2012 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Production of thermostable L2 lipase using recombinant Pichia pastoris

Authors: Shahidan, Noor Hidayah

Year: 2012

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/32372/1/IB%202012%207R%20pdf.pdf

Thesis Expression of human epidermal growth factor in pichia pastoris and optimization of fermentation conditions

Eissazadeh, Samira · 2012 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Expression of human epidermal growth factor in pichia pastoris and optimization of fermentation conditions

Authors: Eissazadeh, Samira

Year: 2012

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/31605/1/FBSB%202012%2030R.pdf

Thesis Molecular characterization and expression of H5, N1 and NS1 recombinant proteins of avian influenza virus subtype H5N1 in Pichia pastoris

Abubakar, Mustapha Bala · 2011 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Molecular characterization and expression of H5, N1 and NS1 recombinant proteins of avian influenza virus subtype H5N1 in Pichia pastoris

Authors: Abubakar, Mustapha Bala

Year: 2011

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/70100/1/FPV%202011%2019%20-%20IR.pdf

Thesis Enhancing recombinant T1 lipase production in Pichia guilliermondii

Ladidi, Abu Mary · 2017 · Universiti Putra Malaysia (PSAS IR) · DOI

Full title: Enhancing recombinant T1 lipase production in Pichia guilliermondii

Authors: Ladidi, Abu Mary

Year: 2017

Venue: Universiti Putra Malaysia (PSAS IR)

DOI: http://psasir.upm.edu.my/id/eprint/70201/1/FBSB%202017%2019%20-%20IR.pdf

Thesis HSF-1, HIF-1and HSP90 expression on recombinant pichia pastoris under fed-batch fermentation

Farías, Jorge G · 2014 · Universidade de São Paulo – USP Digital Library · DOI

Full title: HSF-1, HIF-1and HSP90 expression on recombinant pichia pastoris under fed-batch fermentation

Authors: Farías, Jorge G

Year: 2014

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.scielo.br/pdf/bjm/v45n2/15.pdf

Thesis Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris

Farías, Jorge G · 2014 · Universidade de São Paulo – USP Digital Library · DOI

Full title: Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris

Authors: Farías, Jorge G

Year: 2014

Venue: Universidade de São Paulo – USP Digital Library

DOI: http://www.scielo.br/pdf/bjm/v45n2/14.pdf

Thesis Efeito da adição de oxigênio na produção de glicerol quinase pela levedura recombinante de Pichia pastoris

Toma, Júlio Massao [UNESP] · 2015 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Efeito da adição de oxigênio na produção de glicerol quinase pela levedura recombinante de Pichia pastoris

Authors: Toma, Júlio Massao [UNESP]

Year: 2015

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://hdl.handle.net/11449/121594

Patent us-2003064437-a1

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Paper Enhancement of PVA-degrading enzyme production by the application of pH control strategy.

Li, Min; Zhang, Dongxu; Du, Guocheng et al. · 2012 · Journal of microbiology and biotechnology · DOI

Full title: Enhancement of PVA-degrading enzyme production by the application of pH control strategy.

Authors: Li, Min; Zhang, Dongxu; Du, Guocheng; Chen, Jian

Year: 2012

Venue: Journal of microbiology and biotechnology

DOI: https://doi.org/10.4014/jmb.1107.07030

Thesis Transcriptomic profiling of the yeast Komagataella phaffii in response to environmental alkalinization

Albacar, Marcel; Zekhnini, Abdelghani; Pérez-Valle, Jorge et al. · 2023 · Technical University of Denmark (DTU) · DOI

Full title: Transcriptomic profiling of the yeast Komagataella phaffii in response to environmental alkalinization

Authors: Albacar, Marcel; Zekhnini, Abdelghani; Pérez-Valle, Jorge; Martínez, José L.; id_orcid 0000-0002-4490-8534; Casamayor, Antonio; Ariño, Joaquín

Year: 2023

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/52b4c2a0-bca0-4f1d-9352-cf66a2e956a7

Thesis Effects of different media supplements on the production of an active recombinant plant peroxidase in a Pichia pastoris Δoch1 strain

Gmeiner, Christoph; Spadiut, Oliver · 2015 · ETH Zurich (Swiss Federal Institute of Technology in Zurich) · DOI

Full title: Effects of different media supplements on the production of an active recombinant plant peroxidase in a Pichia pastoris Δoch1 strain

Authors: Gmeiner, Christoph; Spadiut, Oliver

Year: 2015

Venue: ETH Zurich (Swiss Federal Institute of Technology in Zurich)

DOI: http://hdl.handle.net/20.500.11850/107069

Thesis Transcriptomic profiling of the yeast Komagataella phaffii in response to environmental alkalinization

Albacar, Marcel; Zekhnini, Abdelghani; Pérez-Valle, Jorge et al. · 2023 · Technical University of Denmark (DTU) · DOI

Full title: Transcriptomic profiling of the yeast Komagataella phaffii in response to environmental alkalinization

Authors: Albacar, Marcel; Zekhnini, Abdelghani; Pérez-Valle, Jorge; Martínez, José L.; id_orcid 0000-0002-4490-8534; Casamayor, Antonio; Ariño, Joaquín

Year: 2023

Venue: Technical University of Denmark (DTU)

DOI: https://orbit.dtu.dk/en/publications/52b4c2a0-bca0-4f1d-9352-cf66a2e956a7

Thesis Produção de glycerol quinase recombinante utilizando o sistema Pichia pastoris

Terrazas, Werner Damião Morhy; Aizemberg, Raquel [UNESP]; Gattás, Edwil Aparecida de Lucca [UNESP] · 2018 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Produção de glycerol quinase recombinante utilizando o sistema Pichia pastoris

Authors: Terrazas, Werner Damião Morhy; Aizemberg, Raquel [UNESP]; Gattás, Edwil Aparecida de Lucca [UNESP]

Year: 2018

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://hdl.handle.net/11449/177286

Thesis High-Level Expression of a Functional Humanized Anti-CTLA4 Single-Chain Variable Fragment Antibody in Pichia Pastoris

Cai, Huawei; Chen, Lihong; Wan, Lin et al. · 2009 · Hong Kong University of Science and Technology (HKUST) · DOI

Full title: High-Level Expression of a Functional Humanized Anti-CTLA4 Single-Chain Variable Fragment Antibody in Pichia Pastoris

Authors: Cai, Huawei; Chen, Lihong; Wan, Lin; Zeng, Lingyu; Yang, Hao; Li, Shengfu; Li, Youping; Cheng, Jingqiu; Lu, Xiaofeng

Year: 2009

Venue: Hong Kong University of Science and Technology (HKUST)

DOI: http://repository.hkust.edu.hk/ir/Record/1783.1-17754

Thesis Using Pichia pastoris to produce recombinant glycerol kinase

Terrazas, Werner Damião Morhy; Aizemberg, Raquel [UNESP]; Gattas, Edwil Aparecida de Lucca [UNESP] · 2016 · Universidade Estadual Paulista (UNESP) · DOI

Full title: Using Pichia pastoris to produce recombinant glycerol kinase

Authors: Terrazas, Werner Damião Morhy; Aizemberg, Raquel [UNESP]; Gattas, Edwil Aparecida de Lucca [UNESP]

Year: 2016

Venue: Universidade Estadual Paulista (UNESP)

DOI: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/3143

Patent us-2008064084-a1

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2. Data Extraction

0/48 extracted

Thesis

Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso

Russo, Elisa Maria de Sousa · 2017

Full title: Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em sistema Pichia pastoris e otimização do bioprocesso

Authors: Russo, Elisa Maria de Sousa

DOI: https://www.teses.usp.br/teses/disponiveis/60/60135/tde-23112017-101039/

Thesis

Exploring the role of a glycosylated L-asparaginase expressed by a recombinant Pichia pastoris as an antileukemic biopharmaceutical

Pessoa Junior, Adalberto · 2021

Full title: Exploring the role of a glycosylated L-asparaginase expressed by a recombinant Pichia pastoris as an antileukemic biopharmaceutical

Authors: Pessoa Junior, Adalberto

DOI: https://doi.org/10.11606/T.9.2021.tde-21122021-144906

Thesis

Design of a cultivation medium for protein production in Pichia pastoris based on genome-wide biological understanding

Matthews, Catherine Bartlett. · 2019

Full title: Design of a cultivation medium for protein production in Pichia pastoris based on genome-wide biological understanding

Authors: Matthews, Catherine Bartlett.

DOI: https://hdl.handle.net/1721.1/122528

Thesis

Molecular, Genetic, and Process Approaches for Improving Secreted Pharmaceutical Protein Quality in Komagataella phaffii

Yang, Yuchen · 2024

Full title: Molecular, Genetic, and Process Approaches for Improving Secreted Pharmaceutical Protein Quality in Komagataella phaffii

Authors: Yang, Yuchen

DOI: https://hdl.handle.net/1721.1/157251

Thesis

The development and application of mass-spectrometry-based tools to monitor proteome remodeling in microbes

Telusma, Bertina · 2024

Full title: The development and application of mass-spectrometry-based tools to monitor proteome remodeling in microbes

Authors: Telusma, Bertina

DOI: https://hdl.handle.net/1721.1/157155

Thesis

Enhanced Production of Heterologous Protein by Recombinant Aspergillus niger Through Bioprocessing Strategies in Submerged Culture

Wang, Liping · 2002

Full title: Enhanced Production of Heterologous Protein by Recombinant Aspergillus niger Through Bioprocessing Strategies in Submerged Culture

Authors: Wang, Liping

DOI: http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1038425153

Thesis

Produção de glicerol quinase em Pichia pastoris

Aizemberg, Raquel [UNESP] · 2014

Full title: Produção de glicerol quinase em Pichia pastoris

Authors: Aizemberg, Raquel [UNESP]

DOI: http://hdl.handle.net/11449/88349

Thesis

Enhanced biocatalyst production for(R)-phenylacetylcarbinol synthesis

Chen, Allen Kuan-Liang · 2006

Full title: Enhanced biocatalyst production for(R)-phenylacetylcarbinol synthesis

Authors: Chen, Allen Kuan-Liang

DOI: http://hdl.handle.net/1959.4/32825

Thesis

Optimization of the production of recombinant macrophage inhibitory cytokine 1 (MIC-1) from Pichia pastoris

Pratanaphon, Ronachai · 2006

Full title: Optimization of the production of recombinant macrophage inhibitory cytokine 1 (MIC-1) from Pichia pastoris

Authors: Pratanaphon, Ronachai

DOI: http://hdl.handle.net/1959.4/68401

Thesis

A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches

Garcia Ortega, Xavier · 2016

Full title: A step forward in recombinant protein production regulated by the constitutive GAP promoter in Pichia pastoris through bioprocess engineering approaches

Authors: Garcia Ortega, Xavier

DOI: http://hdl.handle.net/10803/384531

Thesis

Development of the production process for a recombinant hormone and evaluation of its biological activity

Font Ingles, Albert · 2013

Full title: Development of the production process for a recombinant hormone and evaluation of its biological activity

Authors: Font Ingles, Albert

DOI: http://hdl.handle.net/10803/123298

Thesis

Development of Pichia pastoris for the production of human α-galactosidase A as a model lysosomal enzyme

Barreiro Vázquez, Antonio · 2016

Full title: Development of Pichia pastoris for the production of human α-galactosidase A as a model lysosomal enzyme

Authors: Barreiro Vázquez, Antonio

DOI: http://hdl.handle.net/10803/382822

Thesis

Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris

Garrigós Martínez, Javier · 2021

Full title: Multidisciplinary approach for recombinant protein production bioprocess design with classic and novel expression systems in Pichia pastoris

Authors: Garrigós Martínez, Javier

DOI: http://hdl.handle.net/10803/671119

Thesis

Overcoming the secretory limitations in Pichia pastoris for recombinant protein production

Barrero Peña, Juan José · 2021

Full title: Overcoming the secretory limitations in Pichia pastoris for recombinant protein production

Authors: Barrero Peña, Juan José

DOI: http://hdl.handle.net/10803/670387

Thesis

Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris

Nieto Taype, Miguel Angel · 2020

Full title: Combining bioprocess and strain engineering strategies as efficient tools for the optimization of recombinant protein production in Pichia pastoris

Authors: Nieto Taype, Miguel Angel

DOI: http://hdl.handle.net/10803/670163

Thesis

Characterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris

Blanchard, V.; Gadkari, R.A.; Gerwig, G.J. et al. · 2007

Full title: Characterization of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris

Authors: Blanchard, V.; Gadkari, R.A.; Gerwig, G.J.; Leeflang, B.R.; Dighe, R.R.; Kamerling, J.P.

DOI: https://dspace.library.uu.nl/handle/1874/27466

Thesis

A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

Lee, Kevin S.; Lee, Harry L. T.; Mozdzierz, Nicholas Joseph et al. · 2017

Full title: A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

Authors: Lee, Kevin S.; Lee, Harry L. T.; Mozdzierz, Nicholas Joseph; Love, Kerry R.; Shah, Kartik A; Ram, Rajeev J; Love, John C

DOI: http://hdl.handle.net/1721.1/109453

Thesis

Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

Dalvie, Neil C; Brady, Joseph R; Crowell, Laura E et al. · 2021

Full title: Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candidate for rotavirus

DOI: https://hdl.handle.net/1721.1/136780

Thesis

Development of a platform process for the production and purification of single‐domain antibodies

Crowell, Laura E; Goodwine, Chaz; Holt, Carla S et al. · 2021

Full title: Development of a platform process for the production and purification of single‐domain antibodies

DOI: https://hdl.handle.net/1721.1/135524

Thesis

Accelerating cell culture media development using Bayesian optimization-based iterative experimental design

Narayanan, Harini; Hinckley, Joshua A; Barry, Rachel et al. · 2025

Full title: Accelerating cell culture media development using Bayesian optimization-based iterative experimental design

Authors: Narayanan, Harini; Hinckley, Joshua A; Barry, Rachel; Dang, Brendan; Wolffe, Lenna A; Atari, Adel; Tseng, Yuen-Yi; Love, J Christopher

DOI: https://hdl.handle.net/1721.1/163220

Thesis

Optimizing Expression and Purification of HaTRPA1

Hansson Wennersten, Wilhelm · 2024

Full title: Optimizing Expression and Purification of HaTRPA1

Authors: Hansson Wennersten, Wilhelm

DOI: http://lup.lub.lu.se/student-papers/record/9159056

Thesis

Development of optimal fermentation expression systems for recombinant proteins: a thesis submitted in partial fulfilment of the requirements for the degree of Masters in Technology at Massey University

Manderson, Daniel · 2017

Authors: Manderson, Daniel

DOI: http://hdl.handle.net/10179/12019

Thesis

Production of thermostable L2 lipase using recombinant Pichia pastoris

Shahidan, Noor Hidayah · 2012

Full title: Production of thermostable L2 lipase using recombinant Pichia pastoris

Authors: Shahidan, Noor Hidayah

DOI: http://psasir.upm.edu.my/id/eprint/32372/1/IB%202012%207R%20pdf.pdf

Thesis

Expression of human epidermal growth factor in pichia pastoris and optimization of fermentation conditions

Eissazadeh, Samira · 2012

Full title: Expression of human epidermal growth factor in pichia pastoris and optimization of fermentation conditions

Authors: Eissazadeh, Samira

DOI: http://psasir.upm.edu.my/id/eprint/31605/1/FBSB%202012%2030R.pdf

Thesis

Molecular characterization and expression of H5, N1 and NS1 recombinant proteins of avian influenza virus subtype H5N1 in Pichia pastoris

Abubakar, Mustapha Bala · 2011

Full title: Molecular characterization and expression of H5, N1 and NS1 recombinant proteins of avian influenza virus subtype H5N1 in Pichia pastoris

Authors: Abubakar, Mustapha Bala

DOI: http://psasir.upm.edu.my/id/eprint/70100/1/FPV%202011%2019%20-%20IR.pdf

Thesis

Enhancing recombinant T1 lipase production in Pichia guilliermondii

Ladidi, Abu Mary · 2017

Full title: Enhancing recombinant T1 lipase production in Pichia guilliermondii

Authors: Ladidi, Abu Mary

DOI: http://psasir.upm.edu.my/id/eprint/70201/1/FBSB%202017%2019%20-%20IR.pdf

Thesis

HSF-1, HIF-1and HSP90 expression on recombinant pichia pastoris under fed-batch fermentation

Farías, Jorge G · 2014

Full title: HSF-1, HIF-1and HSP90 expression on recombinant pichia pastoris under fed-batch fermentation

Authors: Farías, Jorge G

DOI: http://www.scielo.br/pdf/bjm/v45n2/15.pdf

Thesis

Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris

Farías, Jorge G · 2014

Full title: Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris

Authors: Farías, Jorge G

DOI: http://www.scielo.br/pdf/bjm/v45n2/14.pdf

Thesis

Efeito da adição de oxigênio na produção de glicerol quinase pela levedura recombinante de Pichia pastoris

Toma, Júlio Massao [UNESP] · 2015

Full title: Efeito da adição de oxigênio na produção de glicerol quinase pela levedura recombinante de Pichia pastoris

Authors: Toma, Júlio Massao [UNESP]

DOI: http://hdl.handle.net/11449/121594

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Ph Optimization And Its Effects On Protein Quality

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